---
_id: '1028'
abstract:
- lang: eng
  text: Optogenetics and photopharmacology provide spatiotemporally precise control
    over protein interactions and protein function in cells and animals. Optogenetic
    methods that are sensitive to green light and can be used to break protein complexes
    are not broadly available but would enable multichromatic experiments with previously
    inaccessible biological targets. Herein, we repurposed cobalamin (vitamin B12)
    binding domains of bacterial CarH transcription factors for green-light-induced
    receptor dissociation. In cultured cells, we observed oligomerization-induced
    cell signaling for the fibroblast growth factor receptor 1 fused to cobalamin-binding
    domains in the dark that was rapidly eliminated upon illumination. In zebrafish
    embryos expressing fusion receptors, green light endowed control over aberrant
    fibroblast growth factor signaling during development. Green-light-induced domain
    dissociation and light-inactivated receptors will critically expand the optogenetic
    toolbox for control of biological processes.
acknowledgement: "This work was supported by a grant from the European Union\U0010FC1Ds
  Seventh Framework Programme (CIG-303564). E.R. was supported by the graduate program
  MolecularDrugTargets (Austrian Science Fund (FWF), W1232) and a FemTech fellowship
  (Austrian Research Promotion Agency, 3580812)"
article_processing_charge: No
author:
- first_name: Stephanie
  full_name: Kainrath, Stephanie
  id: 32CFBA64-F248-11E8-B48F-1D18A9856A87
  last_name: Kainrath
- first_name: Manuela
  full_name: Stadler, Manuela
  last_name: Stadler
- first_name: Eva
  full_name: Gschaider-Reichhart, Eva
  id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
  last_name: Gschaider-Reichhart
  orcid: 0000-0002-7218-7738
- first_name: Martin
  full_name: Distel, Martin
  last_name: Distel
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
citation:
  ama: Kainrath S, Stadler M, Gschaider-Reichhart E, Distel M, Janovjak HL. Green-light-induced
    inactivation of receptor signaling using cobalamin-binding domains. <i>Angewandte
    Chemie - International Edition</i>. 2017;56(16):4608-4611. doi:<a href="https://doi.org/10.1002/anie.201611998">10.1002/anie.201611998</a>
  apa: Kainrath, S., Stadler, M., Gschaider-Reichhart, E., Distel, M., &#38; Janovjak,
    H. L. (2017). Green-light-induced inactivation of receptor signaling using cobalamin-binding
    domains. <i>Angewandte Chemie - International Edition</i>. Wiley-Blackwell. <a
    href="https://doi.org/10.1002/anie.201611998">https://doi.org/10.1002/anie.201611998</a>
  chicago: Kainrath, Stephanie, Manuela Stadler, Eva Gschaider-Reichhart, Martin Distel,
    and Harald L Janovjak. “Green-Light-Induced Inactivation of Receptor Signaling
    Using Cobalamin-Binding Domains.” <i>Angewandte Chemie - International Edition</i>.
    Wiley-Blackwell, 2017. <a href="https://doi.org/10.1002/anie.201611998">https://doi.org/10.1002/anie.201611998</a>.
  ieee: S. Kainrath, M. Stadler, E. Gschaider-Reichhart, M. Distel, and H. L. Janovjak,
    “Green-light-induced inactivation of receptor signaling using cobalamin-binding
    domains,” <i>Angewandte Chemie - International Edition</i>, vol. 56, no. 16. Wiley-Blackwell,
    pp. 4608–4611, 2017.
  ista: Kainrath S, Stadler M, Gschaider-Reichhart E, Distel M, Janovjak HL. 2017.
    Green-light-induced inactivation of receptor signaling using cobalamin-binding
    domains. Angewandte Chemie - International Edition. 56(16), 4608–4611.
  mla: Kainrath, Stephanie, et al. “Green-Light-Induced Inactivation of Receptor Signaling
    Using Cobalamin-Binding Domains.” <i>Angewandte Chemie - International Edition</i>,
    vol. 56, no. 16, Wiley-Blackwell, 2017, pp. 4608–11, doi:<a href="https://doi.org/10.1002/anie.201611998">10.1002/anie.201611998</a>.
  short: S. Kainrath, M. Stadler, E. Gschaider-Reichhart, M. Distel, H.L. Janovjak,
    Angewandte Chemie - International Edition 56 (2017) 4608–4611.
date_created: 2018-12-11T11:49:46Z
date_published: 2017-03-20T00:00:00Z
date_updated: 2024-03-25T23:30:08Z
day: '20'
ddc:
- '540'
department:
- _id: CaGu
- _id: HaJa
doi: 10.1002/anie.201611998
ec_funded: 1
external_id:
  isi:
  - '000398154000038'
file:
- access_level: open_access
  content_type: application/pdf
  creator: dernst
  date_created: 2019-01-18T09:39:55Z
  date_updated: 2019-01-18T09:39:55Z
  file_id: '5845'
  file_name: 2017_communications_Kainrath.pdf
  file_size: 2614942
  relation: main_file
  success: 1
file_date_updated: 2019-01-18T09:39:55Z
has_accepted_license: '1'
intvolume: '        56'
isi: 1
issue: '16'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '03'
oa: 1
oa_version: Published Version
page: 4608-4611
project:
- _id: 25548C20-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '303564'
  name: Microbial Ion Channels for Synthetic Neurobiology
- _id: 26AA4EF2-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: W1232-B24
  name: Molecular Drug Targets [do not use to be deleted]
publication: Angewandte Chemie - International Edition
publication_identifier:
  issn:
  - '14337851'
publication_status: published
publisher: Wiley-Blackwell
publist_id: '6362'
quality_controlled: '1'
related_material:
  record:
  - id: '418'
    relation: dissertation_contains
    status: public
  - id: '7680'
    relation: part_of_dissertation
    status: public
scopus_import: '1'
status: public
title: Green-light-induced inactivation of receptor signaling using cobalamin-binding
  domains
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 56
year: '2017'
...