[{"publication_status":"published","oa":1,"file_date_updated":"2024-01-10T13:39:42Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":24,"article_processing_charge":"Yes","issue":"22","article_number":"16527","file":[{"checksum":"4df7d206ba022b7f54eff1f0aec1659a","access_level":"open_access","date_updated":"2024-01-10T13:39:42Z","file_size":2637784,"creator":"dernst","file_name":"2023_IJMS_Teplova.pdf","date_created":"2024-01-10T13:39:42Z","success":1,"relation":"main_file","file_id":"14791","content_type":"application/pdf"}],"abstract":[{"lang":"eng","text":"Soluble chaperones residing in the endoplasmic reticulum (ER) play vitally important roles in folding and quality control of newly synthesized proteins that transiently pass through the ER en route to their final destinations. These soluble residents of the ER are themselves endowed with an ER retrieval signal that enables the cell to bring the escaped residents back from the Golgi. Here, by using purified proteins, we showed that Nicotiana tabacum phytaspase, a plant aspartate-specific protease, introduces two breaks at the C-terminus of the N. tabacum ER resident calreticulin-3. These cleavages resulted in removal of either a dipeptide or a hexapeptide from the C-terminus of calreticulin-3 encompassing part or all of the ER retrieval signal. Consistently, expression of the calreticulin-3 derivative mimicking the phytaspase cleavage product in Nicotiana benthamiana cells demonstrated loss of the ER accumulation of the protein. Notably, upon its escape from the ER, calreticulin-3 was further processed by an unknown protease(s) to generate the free N-terminal (N) domain of calreticulin-3, which was ultimately secreted into the apoplast. Our study thus identified a specific proteolytic enzyme capable of precise detachment of the ER retrieval signal from a plant ER resident protein, with implications for the further fate of the escaped resident."}],"_id":"14776","date_published":"2023-11-01T00:00:00Z","external_id":{"pmid":["38003717"],"isi":["001113792600001"]},"date_updated":"2024-01-10T13:41:10Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["1422-0067"]},"article_type":"original","year":"2023","has_accepted_license":"1","oa_version":"Published Version","publisher":"MDPI","isi":1,"publication":"International Journal of Molecular Sciences","department":[{"_id":"JiFr"}],"quality_controlled":"1","intvolume":"        24","status":"public","month":"11","date_created":"2024-01-10T09:24:35Z","pmid":1,"acknowledgement":"We thank C.U.T. Hellen for critically reading the manuscript. The MALDI MS facility and CLSM became available to us in the framework of Moscow State University Development Programs PNG 5.13 and PNR 5.13.\r\nThis work was funded by the Russian Science Foundation, grant numbers 19-14-00010 and 22-14-00071.","keyword":["Inorganic Chemistry","Organic Chemistry","Physical and Theoretical Chemistry","Computer Science Applications","Spectroscopy","Molecular Biology","General Medicine","Catalysis"],"language":[{"iso":"eng"}],"ddc":["580"],"doi":"10.3390/ijms242216527","citation":{"mla":"Teplova, Anastasiia, et al. “Phytaspase Is Capable of Detaching the Endoplasmic Reticulum Retrieval Signal from Tobacco Calreticulin-3.” <i>International Journal of Molecular Sciences</i>, vol. 24, no. 22, 16527, MDPI, 2023, doi:<a href=\"https://doi.org/10.3390/ijms242216527\">10.3390/ijms242216527</a>.","chicago":"Teplova, Anastasiia, Artemii A. Pigidanov, Marina V. Serebryakova, Sergei A. Golyshev, Raisa A. Galiullina, Nina V. Chichkova, and Andrey B. Vartapetian. “Phytaspase Is Capable of Detaching the Endoplasmic Reticulum Retrieval Signal from Tobacco Calreticulin-3.” <i>International Journal of Molecular Sciences</i>. MDPI, 2023. <a href=\"https://doi.org/10.3390/ijms242216527\">https://doi.org/10.3390/ijms242216527</a>.","ista":"Teplova A, Pigidanov AA, Serebryakova MV, Golyshev SA, Galiullina RA, Chichkova NV, Vartapetian AB. 2023. Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3. International Journal of Molecular Sciences. 24(22), 16527.","ieee":"A. Teplova <i>et al.</i>, “Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3,” <i>International Journal of Molecular Sciences</i>, vol. 24, no. 22. MDPI, 2023.","ama":"Teplova A, Pigidanov AA, Serebryakova MV, et al. Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3. <i>International Journal of Molecular Sciences</i>. 2023;24(22). doi:<a href=\"https://doi.org/10.3390/ijms242216527\">10.3390/ijms242216527</a>","apa":"Teplova, A., Pigidanov, A. A., Serebryakova, M. V., Golyshev, S. A., Galiullina, R. A., Chichkova, N. V., &#38; Vartapetian, A. B. (2023). Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms242216527\">https://doi.org/10.3390/ijms242216527</a>","short":"A. Teplova, A.A. Pigidanov, M.V. Serebryakova, S.A. Golyshev, R.A. Galiullina, N.V. Chichkova, A.B. Vartapetian, International Journal of Molecular Sciences 24 (2023)."},"title":"Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3","day":"01","author":[{"last_name":"Teplova","first_name":"Anastasiia","full_name":"Teplova, Anastasiia","id":"e3736151-106c-11ec-b916-c2558e2762c6"},{"last_name":"Pigidanov","full_name":"Pigidanov, Artemii A.","first_name":"Artemii A."},{"last_name":"Serebryakova","first_name":"Marina V.","full_name":"Serebryakova, Marina V."},{"last_name":"Golyshev","first_name":"Sergei A.","full_name":"Golyshev, Sergei A."},{"last_name":"Galiullina","full_name":"Galiullina, Raisa A.","first_name":"Raisa A."},{"last_name":"Chichkova","first_name":"Nina V.","full_name":"Chichkova, Nina V."},{"last_name":"Vartapetian","first_name":"Andrey B.","full_name":"Vartapetian, Andrey B."}],"type":"journal_article"},{"page":"6352","date_created":"2022-07-05T15:14:34Z","month":"06","isi":1,"publisher":"MDPI","intvolume":"        23","status":"public","publication":"International Journal of Molecular Sciences","quality_controlled":"1","department":[{"_id":"JiFr"}],"title":"The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier harbors hallmarks of an intrinsically disordered protein","citation":{"mla":"Bilanovičová, V., et al. “The Hydrophilic Loop of Arabidopsis PIN1 Auxin Efflux Carrier Harbors Hallmarks of an Intrinsically Disordered Protein.” <i>International Journal of Molecular Sciences</i>, vol. 23, no. 11, MDPI, 2022, p. 6352, doi:<a href=\"https://doi.org/10.3390/ijms23116352\">10.3390/ijms23116352</a>.","chicago":"Bilanovičová, V, N Rýdza, L Koczka, M Hess, E Feraru, Jiří Friml, and T Nodzyński. “The Hydrophilic Loop of Arabidopsis PIN1 Auxin Efflux Carrier Harbors Hallmarks of an Intrinsically Disordered Protein.” <i>International Journal of Molecular Sciences</i>. MDPI, 2022. <a href=\"https://doi.org/10.3390/ijms23116352\">https://doi.org/10.3390/ijms23116352</a>.","ista":"Bilanovičová V, Rýdza N, Koczka L, Hess M, Feraru E, Friml J, Nodzyński T. 2022. The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier harbors hallmarks of an intrinsically disordered protein. International Journal of Molecular Sciences. 23(11), 6352.","ieee":"V. Bilanovičová <i>et al.</i>, “The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier harbors hallmarks of an intrinsically disordered protein,” <i>International Journal of Molecular Sciences</i>, vol. 23, no. 11. MDPI, p. 6352, 2022.","ama":"Bilanovičová V, Rýdza N, Koczka L, et al. The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier harbors hallmarks of an intrinsically disordered protein. <i>International Journal of Molecular Sciences</i>. 2022;23(11):6352. doi:<a href=\"https://doi.org/10.3390/ijms23116352\">10.3390/ijms23116352</a>","apa":"Bilanovičová, V., Rýdza, N., Koczka, L., Hess, M., Feraru, E., Friml, J., &#38; Nodzyński, T. (2022). The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier harbors hallmarks of an intrinsically disordered protein. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms23116352\">https://doi.org/10.3390/ijms23116352</a>","short":"V. Bilanovičová, N. Rýdza, L. Koczka, M. Hess, E. Feraru, J. Friml, T. Nodzyński, International Journal of Molecular Sciences 23 (2022) 6352."},"author":[{"full_name":"Bilanovičová, V","first_name":"V","last_name":"Bilanovičová"},{"last_name":"Rýdza","first_name":"N","full_name":"Rýdza, N"},{"last_name":"Koczka","first_name":"L","full_name":"Koczka, L"},{"first_name":"M","full_name":"Hess, M","last_name":"Hess"},{"last_name":"Feraru","full_name":"Feraru, E","first_name":"E"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml"},{"last_name":"Nodzyński","first_name":"T","full_name":"Nodzyński, T"}],"type":"journal_article","day":"06","pmid":1,"project":[{"name":"RNA-directed DNA methylation in plant development","grant_number":"P29988","call_identifier":"FWF","_id":"262EF96E-B435-11E9-9278-68D0E5697425"}],"acknowledgement":"We thank Charo del Genio from Coventry University and Richard Napier from the University of Warwick for helpful discussion concerning protein modeling and inspiration concerning CD spectroscopy, respectively. We thank Jan Hejatko for sharing the published AHP2 construct. We also thank Josef Houser from the core facility BIC CEITEC for valuable assistance, discussions, and ideas relating to CD. We acknowledge the: Core Facility CELLIM of CEITEC supported by the Czech-BioImaging large RI project (LM2018129 funded by MEYS CR), part of the Euro-BioImaging (www.eurobioimaging.eu accessed on 1 January 2016) ALM and medical imaging Node (Brno, CZ), CF Biomolecular Interactions and Crystallization of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127) and European Regional Development Fund-Project “UP CIISB“ (No. CZ.02.1.01/0.0/0.0/18_046/0015974) for their support with obtaining scientific data presented in this paper; Plant Sciences Core Facility of CEITEC Masaryk University for technical support. Open Access Funding by the Austrian Science Fund (FWF).","doi":"10.3390/ijms23116352","ddc":["570"],"language":[{"iso":"eng"}],"article_processing_charge":"Yes","issue":"11","abstract":[{"lang":"eng","text":"Much of plant development depends on cell-to-cell redistribution of the plant hormone auxin, which is facilitated by the plasma membrane (PM) localized PIN FORMED (PIN) proteins. Auxin export activity, developmental roles, subcellular trafficking, and polarity of PINs have been well studied, but their structure remains elusive besides a rough outline that they contain two groups of 5 alpha-helices connected by a large hydrophilic loop (HL). Here, we focus on the PIN1 HL as we could produce it in sufficient quantities for biochemical investigations to provide insights into its secondary structure. Circular dichroism (CD) studies revealed its nature as an intrinsically disordered protein (IDP), manifested by the increase of structure content upon thermal melting. Consistent with IDPs serving as interaction platforms, PIN1 loops homodimerize. PIN1 HL cytoplasmic overexpression in Arabidopsis disrupts early endocytic trafficking of PIN1 and PIN2 and causes defects in the cotyledon vasculature formation. In summary, we demonstrate that PIN1 HL has an intrinsically disordered nature, which must be considered to gain further structural insights. Some secondary structures may form transiently during pairing with known and yet-to-be-discovered interactors."}],"_id":"11489","date_published":"2022-06-06T00:00:00Z","file":[{"access_level":"open_access","date_updated":"2022-07-06T07:36:59Z","checksum":"e997a57a928ec9d51fad8ce824a05935","file_size":2324542,"creator":"cchlebak","file_name":"2022_IntJMolSci_Bilanovicova.pdf","success":1,"date_created":"2022-07-06T07:36:59Z","relation":"main_file","file_id":"11492","content_type":"application/pdf"}],"oa":1,"publication_status":"published","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":23,"file_date_updated":"2022-07-06T07:36:59Z","has_accepted_license":"1","year":"2022","oa_version":"Published Version","article_type":"original","publication_identifier":{"issn":["1422-0067"]},"external_id":{"isi":["000808733300001"],"pmid":["35683031"]},"date_updated":"2023-08-09T10:13:57Z","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87"},{"oa":1,"publication_status":"published","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":21,"file_date_updated":"2020-09-10T07:06:22Z","article_processing_charge":"No","issue":"16","date_published":"2020-08-08T00:00:00Z","_id":"8225","abstract":[{"text":"Birch pollen allergy is among the most prevalent pollen allergies in Northern and Central Europe. This IgE-mediated disease can be treated with allergen immunotherapy (AIT), which typically gives rise to IgG antibodies inducing tolerance. Although the main mechanisms of allergen immunotherapy (AIT) are known, questions regarding possible Fc-mediated effects of IgG antibodies remain unanswered. This can mainly be attributed to the unavailability of appropriate tools, i.e., well-characterised recombinant antibodies (rAbs). We hereby aimed at providing human rAbs of several classes for mechanistic studies and as possible candidates for passive immunotherapy. We engineered IgE, IgG1, and IgG4 sharing the same variable region against the major birch pollen allergen Bet v 1 using Polymerase Incomplete Primer Extension (PIPE) cloning. We tested IgE functionality and IgG blocking capabilities using appropriate model cell lines. In vitro studies showed IgE engagement with FcεRI and CD23 and Bet v 1-dependent degranulation. Overall, we hereby present fully functional, human IgE, IgG1, and IgG4 sharing the same variable region against Bet v 1 and showcase possible applications in first mechanistic studies. Furthermore, our IgG antibodies might be useful candidates for passive immunotherapy of birch pollen allergy.","lang":"eng"}],"article_number":"5693","file":[{"file_name":"2020_IntMolecSciences_Koehler.pdf","creator":"dernst","file_size":2680908,"checksum":"dac7ccef7cdcea9be292664d8c488425","date_updated":"2020-09-10T07:06:22Z","access_level":"open_access","content_type":"application/pdf","relation":"main_file","file_id":"8356","date_created":"2020-09-10T07:06:22Z","success":1}],"publication_identifier":{"issn":["1422-0067"]},"external_id":{"pmid":["32784509"]},"date_updated":"2021-01-12T08:17:34Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2020","has_accepted_license":"1","oa_version":"Published Version","article_type":"original","publisher":"MDPI","status":"public","intvolume":"        21","publication":"International Journal of Molecular Sciences","quality_controlled":"1","date_created":"2020-08-10T11:47:29Z","month":"08","extern":"1","pmid":1,"ddc":["570"],"doi":"10.3390/ijms21165693","language":[{"iso":"eng"}],"title":"Filling the antibody pipeline in allergy: PIPE cloning of IgE, IgG1 and IgG4 against the major birch pollen allergen Bet v 1","citation":{"ama":"Köhler VK, Crescioli S, Singer J, et al. Filling the antibody pipeline in allergy: PIPE cloning of IgE, IgG1 and IgG4 against the major birch pollen allergen Bet v 1. <i>International Journal of Molecular Sciences</i>. 2020;21(16). doi:<a href=\"https://doi.org/10.3390/ijms21165693\">10.3390/ijms21165693</a>","apa":"Köhler, V. K., Crescioli, S., Singer, J., Bax, H. J., Hofer, G., Pranger, C. L., … Jensen-Jarolim, E. (2020). Filling the antibody pipeline in allergy: PIPE cloning of IgE, IgG1 and IgG4 against the major birch pollen allergen Bet v 1. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms21165693\">https://doi.org/10.3390/ijms21165693</a>","short":"V.K. Köhler, S. Crescioli, J. Singer, H.J. Bax, G. Hofer, C.L. Pranger, K. Hufnagl, R. Bianchini, S. Flicker, W. Keller, S.N. Karagiannis, E. Jensen-Jarolim, International Journal of Molecular Sciences 21 (2020).","mla":"Köhler, Verena K., et al. “Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG1 and IgG4 against the Major Birch Pollen Allergen Bet v 1.” <i>International Journal of Molecular Sciences</i>, vol. 21, no. 16, 5693, MDPI, 2020, doi:<a href=\"https://doi.org/10.3390/ijms21165693\">10.3390/ijms21165693</a>.","chicago":"Köhler, Verena K., Silvia Crescioli, Judit Singer, Heather J. Bax, Gerhard Hofer, Christina L. Pranger, Karin Hufnagl, et al. “Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG1 and IgG4 against the Major Birch Pollen Allergen Bet v 1.” <i>International Journal of Molecular Sciences</i>. MDPI, 2020. <a href=\"https://doi.org/10.3390/ijms21165693\">https://doi.org/10.3390/ijms21165693</a>.","ista":"Köhler VK, Crescioli S, Singer J, Bax HJ, Hofer G, Pranger CL, Hufnagl K, Bianchini R, Flicker S, Keller W, Karagiannis SN, Jensen-Jarolim E. 2020. Filling the antibody pipeline in allergy: PIPE cloning of IgE, IgG1 and IgG4 against the major birch pollen allergen Bet v 1. International Journal of Molecular Sciences. 21(16), 5693.","ieee":"V. K. Köhler <i>et al.</i>, “Filling the antibody pipeline in allergy: PIPE cloning of IgE, IgG1 and IgG4 against the major birch pollen allergen Bet v 1,” <i>International Journal of Molecular Sciences</i>, vol. 21, no. 16. MDPI, 2020."},"type":"journal_article","author":[{"orcid":"0000-0001-5581-398X","full_name":"Köhler, Verena K.","first_name":"Verena K.","last_name":"Köhler"},{"last_name":"Crescioli","full_name":"Crescioli, Silvia","first_name":"Silvia","orcid":"0000-0002-1909-5957"},{"orcid":"0000-0002-8777-3502","id":"36432834-F248-11E8-B48F-1D18A9856A87","full_name":"Fazekas-Singer, Judit","first_name":"Judit","last_name":"Fazekas-Singer"},{"orcid":"0000-0003-0432-4160","last_name":"Bax","full_name":"Bax, Heather J.","first_name":"Heather J."},{"last_name":"Hofer","first_name":"Gerhard","full_name":"Hofer, Gerhard"},{"first_name":"Christina L.","full_name":"Pranger, Christina L.","last_name":"Pranger"},{"full_name":"Hufnagl, Karin","first_name":"Karin","last_name":"Hufnagl"},{"last_name":"Bianchini","first_name":"Rodolfo","full_name":"Bianchini, Rodolfo","orcid":"0000-0003-0351-6937"},{"orcid":"0000-0003-4768-8693","last_name":"Flicker","first_name":"Sabine","full_name":"Flicker, Sabine"},{"last_name":"Keller","full_name":"Keller, Walter","first_name":"Walter","orcid":"0000-0002-2261-958X"},{"last_name":"Karagiannis","first_name":"Sophia N.","full_name":"Karagiannis, Sophia N.","orcid":"0000-0002-4100-7810"},{"last_name":"Jensen-Jarolim","full_name":"Jensen-Jarolim, Erika","first_name":"Erika","orcid":"0000-0003-4019-5765"}],"day":"08"}]