---
_id: '14653'
abstract:
- lang: eng
  text: 'Mass spectrometry imaging (MSI) is a powerful analytical technique for the
    two-dimensional (2D) localization of chemicals on surfaces. Conventional MSI experiments
    require to predefine the surface of interest based on photographic or microscopic
    images. Typically, these boundaries can no longer be changed or adjusted once
    the experiment has been started. In terms of a more interactive approach we recently
    developed a pen-like ionization interface which is directly connected to the mass
    spectrometer. The device allows the user to ionize chemicals by desorption electrospray
    ionization (DESI) and to freely move the interface over a surface of interest.
    A mini camera, which is mounted on the tip of the pen, magnifies the desorption
    area and enables a simple positioning of the pen. The combination of optical data
    from the camera module and chemical data obtained by mass analysis facilitates
    a novel type of imaging experiment: interactive mass spectrometry imaging (IMSI).
    For this application, we present a novel approach for a robust, optical flow-based
    motion detection. While the live video stream from the camera is used to track
    the pen''s motion across the surface a post-acquisition algorithm correlates the
    coordinates of the pen trajectory with respective mass spectra obtained from a
    simultaneous mass spectrometric data acquisition. This algorithm is no longer
    dependent on a single, manually applied optical marker on the sample surface,
    which has to be visible on all video frames throughout the analysis. The advanced
    DESI-IMSI method was successfully tested on inkjet-printed letters as well as
    mouse brain tissue samples. Validation of the results was done by comparing DESI-IMSI
    with standard DESI-MSI data.'
acknowledgement: We would like to thank Marco Sealey Cardona, PhD for help with the
  mouse brain samples and acknowledge the financial support by 1669 Förderkreis of
  the University of Innsbruck, Austria Wirtschaftsservice (AWS), D. Swarovski KG and
  Tyrolean Science Fund (TWF).
article_number: '117168'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Florian
  full_name: Kluibenschedl, Florian
  id: 7499e70e-eb2c-11ec-b98b-f925648bc9d9
  last_name: Kluibenschedl
- first_name: Anna
  full_name: Ploner, Anna
  last_name: Ploner
- first_name: Christina
  full_name: Meisenbichler, Christina
  last_name: Meisenbichler
- first_name: Robert
  full_name: Konrat, Robert
  last_name: Konrat
- first_name: Thomas
  full_name: Müller, Thomas
  last_name: Müller
citation:
  ama: Kluibenschedl F, Ploner A, Meisenbichler C, Konrat R, Müller T. Advanced motion
    tracking for interactive mass spectrometry imaging (IMSI). <i>International Journal
    of Mass Spectrometry</i>. 2023;495. doi:<a href="https://doi.org/10.1016/j.ijms.2023.117168">10.1016/j.ijms.2023.117168</a>
  apa: Kluibenschedl, F., Ploner, A., Meisenbichler, C., Konrat, R., &#38; Müller,
    T. (2023). Advanced motion tracking for interactive mass spectrometry imaging
    (IMSI). <i>International Journal of Mass Spectrometry</i>. Elsevier. <a href="https://doi.org/10.1016/j.ijms.2023.117168">https://doi.org/10.1016/j.ijms.2023.117168</a>
  chicago: Kluibenschedl, Florian, Anna Ploner, Christina Meisenbichler, Robert Konrat,
    and Thomas Müller. “Advanced Motion Tracking for Interactive Mass Spectrometry
    Imaging (IMSI).” <i>International Journal of Mass Spectrometry</i>. Elsevier,
    2023. <a href="https://doi.org/10.1016/j.ijms.2023.117168">https://doi.org/10.1016/j.ijms.2023.117168</a>.
  ieee: F. Kluibenschedl, A. Ploner, C. Meisenbichler, R. Konrat, and T. Müller, “Advanced
    motion tracking for interactive mass spectrometry imaging (IMSI),” <i>International
    Journal of Mass Spectrometry</i>, vol. 495. Elsevier, 2023.
  ista: Kluibenschedl F, Ploner A, Meisenbichler C, Konrat R, Müller T. 2023. Advanced
    motion tracking for interactive mass spectrometry imaging (IMSI). International
    Journal of Mass Spectrometry. 495, 117168.
  mla: Kluibenschedl, Florian, et al. “Advanced Motion Tracking for Interactive Mass
    Spectrometry Imaging (IMSI).” <i>International Journal of Mass Spectrometry</i>,
    vol. 495, 117168, Elsevier, 2023, doi:<a href="https://doi.org/10.1016/j.ijms.2023.117168">10.1016/j.ijms.2023.117168</a>.
  short: F. Kluibenschedl, A. Ploner, C. Meisenbichler, R. Konrat, T. Müller, International
    Journal of Mass Spectrometry 495 (2023).
dataavailabilitystatement: Data will be made available on request.
date_created: 2023-12-10T23:00:57Z
date_published: 2023-11-23T00:00:00Z
date_updated: 2026-03-02T09:38:59Z
day: '23'
department:
- _id: GradSch
doi: 10.1016/j.ijms.2023.117168
intvolume: '       495'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.ijms.2023.117168
month: '11'
oa: 1
oa_version: Published Version
publication: International Journal of Mass Spectrometry
publication_identifier:
  issn:
  - 1387-3806
publication_status: epub_ahead
publisher: Elsevier
quality_controlled: '1'
researchdata_availability: upon request
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Advanced motion tracking for interactive mass spectrometry imaging (IMSI)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 495
year: '2023'
...