---
_id: '9009'
abstract:
- lang: eng
  text: Recent advancements in live cell imaging technologies have identified the
    phenomenon of intracellular propagation of late apoptotic events, such as cytochrome
    c release and caspase activation. The mechanism, prevalence, and speed of apoptosis
    propagation remain unclear. Additionally, no studies have demonstrated propagation
    of the pro-apoptotic protein, BAX. To evaluate the role of BAX in intracellular
    apoptotic propagation, we used high speed live-cell imaging to visualize fluorescently
    tagged-BAX recruitment to mitochondria in four immortalized cell lines. We show
    that propagation of mitochondrial BAX recruitment occurs in parallel to cytochrome
    c and SMAC/Diablo release and is affected by cellular morphology, such that cells
    with processes are more likely to exhibit propagation. The initiation of propagation
    events is most prevalent in the distal tips of processes, while the rate of propagation
    is influenced by the 2-dimensional width of the process. Propagation was rarely
    observed in the cell soma, which exhibited near synchronous recruitment of BAX.
    Propagation velocity is not affected by mitochondrial volume in segments of processes,
    but is negatively affected by mitochondrial density. There was no evidence of
    a propagating wave of increased levels of intracellular calcium ions. Alternatively,
    we did observe a uniform increase in superoxide build-up in cellular mitochondria,
    which was released as a propagating wave simultaneously with the propagating recruitment
    of BAX to the mitochondrial outer membrane.
acknowledgement: This work was supported by National Institute of Health grants R01
  EY030123, P30 EY016665, and T32 GM081061, an unrestricted research grant from Research
  to Prevent Blindness, Inc., and the Frederick A. Davis Endowment from the Department
  of Ophthalmology and Visual Sciences at the University of Wisconsin-Madison.
article_processing_charge: No
article_type: original
author:
- first_name: Joshua A.
  full_name: Grosser, Joshua A.
  last_name: Grosser
- first_name: Margaret E
  full_name: Maes, Margaret E
  id: 3838F452-F248-11E8-B48F-1D18A9856A87
  last_name: Maes
  orcid: 0000-0001-9642-1085
- first_name: Robert W.
  full_name: Nickells, Robert W.
  last_name: Nickells
citation:
  ama: Grosser JA, Maes ME, Nickells RW. Characteristics of intracellular propagation
    of mitochondrial BAX recruitment during apoptosis. <i>Apoptosis</i>. 2021;26(2):132-145.
    doi:<a href="https://doi.org/10.1007/s10495-020-01654-w">10.1007/s10495-020-01654-w</a>
  apa: Grosser, J. A., Maes, M. E., &#38; Nickells, R. W. (2021). Characteristics
    of intracellular propagation of mitochondrial BAX recruitment during apoptosis.
    <i>Apoptosis</i>. Springer Nature. <a href="https://doi.org/10.1007/s10495-020-01654-w">https://doi.org/10.1007/s10495-020-01654-w</a>
  chicago: Grosser, Joshua A., Margaret E Maes, and Robert W. Nickells. “Characteristics
    of Intracellular Propagation of Mitochondrial BAX Recruitment during Apoptosis.”
    <i>Apoptosis</i>. Springer Nature, 2021. <a href="https://doi.org/10.1007/s10495-020-01654-w">https://doi.org/10.1007/s10495-020-01654-w</a>.
  ieee: J. A. Grosser, M. E. Maes, and R. W. Nickells, “Characteristics of intracellular
    propagation of mitochondrial BAX recruitment during apoptosis,” <i>Apoptosis</i>,
    vol. 26, no. 2. Springer Nature, pp. 132–145, 2021.
  ista: Grosser JA, Maes ME, Nickells RW. 2021. Characteristics of intracellular propagation
    of mitochondrial BAX recruitment during apoptosis. Apoptosis. 26(2), 132–145.
  mla: Grosser, Joshua A., et al. “Characteristics of Intracellular Propagation of
    Mitochondrial BAX Recruitment during Apoptosis.” <i>Apoptosis</i>, vol. 26, no.
    2, Springer Nature, 2021, pp. 132–45, doi:<a href="https://doi.org/10.1007/s10495-020-01654-w">10.1007/s10495-020-01654-w</a>.
  short: J.A. Grosser, M.E. Maes, R.W. Nickells, Apoptosis 26 (2021) 132–145.
date_created: 2021-01-17T23:01:11Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2023-08-07T13:32:40Z
day: '01'
department:
- _id: SaSi
doi: 10.1007/s10495-020-01654-w
external_id:
  isi:
  - '000606722600001'
  pmid:
  - '33426618'
intvolume: '        26'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082518/
month: '02'
oa: 1
oa_version: Submitted Version
page: 132-145
pmid: 1
publication: Apoptosis
publication_identifier:
  eissn:
  - 1573-675X
  issn:
  - 1360-8185
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Characteristics of intracellular propagation of mitochondrial BAX recruitment
  during apoptosis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 26
year: '2021'
...