---
_id: '12119'
abstract:
- lang: eng
  text: Intravascular neutrophils and platelets collaborate in maintaining host integrity,
    but their interaction can also trigger thrombotic complications. We report here
    that cooperation between neutrophil and platelet lineages extends to the earliest
    stages of platelet formation by megakaryocytes in the bone marrow. Using intravital
    microscopy, we show that neutrophils “plucked” intravascular megakaryocyte extensions,
    termed proplatelets, to control platelet production. Following CXCR4-CXCL12-dependent
    migration towards perisinusoidal megakaryocytes, plucking neutrophils actively
    pulled on proplatelets and triggered myosin light chain and extracellular-signal-regulated
    kinase activation through reactive oxygen species. By these mechanisms, neutrophils
    accelerate proplatelet growth and facilitate continuous release of platelets in
    steady state. Following myocardial infarction, plucking neutrophils drove excessive
    release of young, reticulated platelets and boosted the risk of recurrent ischemia.
    Ablation of neutrophil plucking normalized thrombopoiesis and reduced recurrent
    thrombosis after myocardial infarction and thrombus burden in venous thrombosis.
    We establish neutrophil plucking as a target to reduce thromboischemic events.
acknowledgement: "We thank Coung Kieu and Dominik van den Heuvel for excellent technical
  assistance. This work was supported by the German Research Foundation (PE2704/2-1,
  PE2704/3-1 to T.P., SFB 1123-project B06 to S.M., SFB1525 project A07 to D.S, TRR
  332 project A7 to C.S., PO 2247/2-1 to A.P., SFB1116-project B11 to A.P. and B12
  to M.K.), LMU Munich’s Institutional\r\nStrategy LMUexcellent within the framework
  of the German Excellence Initiative (No. 806 32 006 to T.P.), and by the German
  Centre for Cardiovascular Research (DZHK) to T.P. (Postdoc Start-up grant No. 100378833).
  This project has received funding from the European Research Council (ERC) under
  the European Union’s Horizon 2020 research and innovation program (grant agreement
  No. 833440 to S.M.). F.G. received funding from the European Union’s\r\nHorizon
  2020 research and innovation program under the Marie Sk1odowska-Curie grant agreement
  no. 747687. A.H. was funded by RTI2018-095497-B-I00 from Ministerio de Ciencia e
  Innovacio´ n (MICINN), HR17_00527 from Fundacion La Caixa, and Transatlantic Network
  of Excellence (TNE-18CVD04) from the Leducq Foundation. The CNIC is supported by
  the MICINN and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence
  (CEX2020-001041-S). A.P. was supported by the Forschungskommission of the Medical
  Faculty of the Heinrich-Heine-Universität Düsseldorf (No. 18-2019 to A.P.). C.G.
  was supported by the Helmholtz Alliance ‘Aging and Metabolic Programming, AMPro,’
  by the German Federal\r\nMinistry of Education and Research to the German Center
  for Diabetes Research (DZD), and by the Bavarian State Ministry of Health and Care
  through the research project DigiMed Bayern."
article_processing_charge: No
article_type: original
author:
- first_name: Tobias
  full_name: Petzold, Tobias
  last_name: Petzold
- first_name: Zhe
  full_name: Zhang, Zhe
  last_name: Zhang
- first_name: Iván
  full_name: Ballesteros, Iván
  last_name: Ballesteros
- first_name: Inas
  full_name: Saleh, Inas
  last_name: Saleh
- first_name: Amin
  full_name: Polzin, Amin
  last_name: Polzin
- first_name: Manuela
  full_name: Thienel, Manuela
  last_name: Thienel
- first_name: Lulu
  full_name: Liu, Lulu
  last_name: Liu
- first_name: Qurrat
  full_name: Ul Ain, Qurrat
  last_name: Ul Ain
- first_name: Vincent
  full_name: Ehreiser, Vincent
  last_name: Ehreiser
- first_name: Christian
  full_name: Weber, Christian
  last_name: Weber
- first_name: Badr
  full_name: Kilani, Badr
  last_name: Kilani
- first_name: Pontus
  full_name: Mertsch, Pontus
  last_name: Mertsch
- first_name: Jeremias
  full_name: Götschke, Jeremias
  last_name: Götschke
- first_name: Sophie
  full_name: Cremer, Sophie
  last_name: Cremer
- first_name: Wenwen
  full_name: Fu, Wenwen
  last_name: Fu
- first_name: Michael
  full_name: Lorenz, Michael
  last_name: Lorenz
- first_name: Hellen
  full_name: Ishikawa-Ankerhold, Hellen
  last_name: Ishikawa-Ankerhold
- first_name: Elisabeth
  full_name: Raatz, Elisabeth
  last_name: Raatz
- first_name: Shaza
  full_name: El-Nemr, Shaza
  last_name: El-Nemr
- first_name: Agnes
  full_name: Görlach, Agnes
  last_name: Görlach
- first_name: Esther
  full_name: Marhuenda, Esther
  last_name: Marhuenda
- first_name: Konstantin
  full_name: Stark, Konstantin
  last_name: Stark
- first_name: Joachim
  full_name: Pircher, Joachim
  last_name: Pircher
- first_name: David
  full_name: Stegner, David
  last_name: Stegner
- first_name: Christian
  full_name: Gieger, Christian
  last_name: Gieger
- first_name: Marc
  full_name: Schmidt-Supprian, Marc
  last_name: Schmidt-Supprian
- first_name: Florian R
  full_name: Gärtner, Florian R
  id: 397A88EE-F248-11E8-B48F-1D18A9856A87
  last_name: Gärtner
  orcid: 0000-0001-6120-3723
- first_name: Isaac
  full_name: Almendros, Isaac
  last_name: Almendros
- first_name: Malte
  full_name: Kelm, Malte
  last_name: Kelm
- first_name: Christian
  full_name: Schulz, Christian
  last_name: Schulz
- first_name: Andrés
  full_name: Hidalgo, Andrés
  last_name: Hidalgo
- first_name: Steffen
  full_name: Massberg, Steffen
  last_name: Massberg
citation:
  ama: Petzold T, Zhang Z, Ballesteros I, et al. Neutrophil “plucking” on megakaryocytes
    drives platelet production and boosts cardiovascular disease. <i>Immunity</i>.
    2022;55(12):2285-2299.e7. doi:<a href="https://doi.org/10.1016/j.immuni.2022.10.001">10.1016/j.immuni.2022.10.001</a>
  apa: Petzold, T., Zhang, Z., Ballesteros, I., Saleh, I., Polzin, A., Thienel, M.,
    … Massberg, S. (2022). Neutrophil “plucking” on megakaryocytes drives platelet
    production and boosts cardiovascular disease. <i>Immunity</i>. Elsevier. <a href="https://doi.org/10.1016/j.immuni.2022.10.001">https://doi.org/10.1016/j.immuni.2022.10.001</a>
  chicago: Petzold, Tobias, Zhe Zhang, Iván Ballesteros, Inas Saleh, Amin Polzin,
    Manuela Thienel, Lulu Liu, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives
    Platelet Production and Boosts Cardiovascular Disease.” <i>Immunity</i>. Elsevier,
    2022. <a href="https://doi.org/10.1016/j.immuni.2022.10.001">https://doi.org/10.1016/j.immuni.2022.10.001</a>.
  ieee: T. Petzold <i>et al.</i>, “Neutrophil ‘plucking’ on megakaryocytes drives
    platelet production and boosts cardiovascular disease,” <i>Immunity</i>, vol.
    55, no. 12. Elsevier, p. 2285–2299.e7, 2022.
  ista: Petzold T, Zhang Z, Ballesteros I, Saleh I, Polzin A, Thienel M, Liu L, Ul
    Ain Q, Ehreiser V, Weber C, Kilani B, Mertsch P, Götschke J, Cremer S, Fu W, Lorenz
    M, Ishikawa-Ankerhold H, Raatz E, El-Nemr S, Görlach A, Marhuenda E, Stark K,
    Pircher J, Stegner D, Gieger C, Schmidt-Supprian M, Gärtner FR, Almendros I, Kelm
    M, Schulz C, Hidalgo A, Massberg S. 2022. Neutrophil “plucking” on megakaryocytes
    drives platelet production and boosts cardiovascular disease. Immunity. 55(12),
    2285–2299.e7.
  mla: Petzold, Tobias, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives Platelet
    Production and Boosts Cardiovascular Disease.” <i>Immunity</i>, vol. 55, no. 12,
    Elsevier, 2022, p. 2285–2299.e7, doi:<a href="https://doi.org/10.1016/j.immuni.2022.10.001">10.1016/j.immuni.2022.10.001</a>.
  short: T. Petzold, Z. Zhang, I. Ballesteros, I. Saleh, A. Polzin, M. Thienel, L.
    Liu, Q. Ul Ain, V. Ehreiser, C. Weber, B. Kilani, P. Mertsch, J. Götschke, S.
    Cremer, W. Fu, M. Lorenz, H. Ishikawa-Ankerhold, E. Raatz, S. El-Nemr, A. Görlach,
    E. Marhuenda, K. Stark, J. Pircher, D. Stegner, C. Gieger, M. Schmidt-Supprian,
    F.R. Gärtner, I. Almendros, M. Kelm, C. Schulz, A. Hidalgo, S. Massberg, Immunity
    55 (2022) 2285–2299.e7.
date_created: 2023-01-12T11:56:54Z
date_published: 2022-12-13T00:00:00Z
date_updated: 2023-08-03T14:21:51Z
day: '13'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1016/j.immuni.2022.10.001
ec_funded: 1
external_id:
  isi:
  - '000922019600003'
  pmid:
  - '36272416'
file:
- access_level: open_access
  checksum: 073267a9c0ad9f85a650053bc7b23777
  content_type: application/pdf
  creator: dernst
  date_created: 2023-01-23T10:18:48Z
  date_updated: 2023-01-23T10:18:48Z
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file_date_updated: 2023-01-23T10:18:48Z
has_accepted_license: '1'
intvolume: '        55'
isi: 1
issue: '12'
keyword:
- Infectious Diseases
- Immunology
- Immunology and Allergy
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 2285-2299.e7
pmid: 1
project:
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '747687'
  name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
publication: Immunity
publication_identifier:
  issn:
  - 1074-7613
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Neutrophil “plucking” on megakaryocytes drives platelet production and boosts
  cardiovascular disease
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 55
year: '2022'
...