---
_id: '698'
abstract:
- lang: eng
  text: 'Extracellular matrix signals from the microenvironment regulate gene expression
    patterns and cell behavior. Using a combination of experiments and geometric models,
    we demonstrate correlations between cell geometry, three-dimensional (3D) organization
    of chromosome territories, and gene expression. Fluorescence in situ hybridization
    experiments showed that micropatterned fibroblasts cultured on anisotropic versus
    isotropic substrates resulted in repositioning of specific chromosomes, which
    contained genes that were differentially regulated by cell geometries. Experiments
    combined with ellipsoid packing models revealed that the mechanosensitivity of
    chromosomes was correlated with their orientation in the nucleus. Transcription
    inhibition experiments suggested that the intermingling degree was more sensitive
    to global changes in transcription than to chromosome radial positioning and its
    orientations. These results suggested that cell geometry modulated 3D chromosome
    arrangement, and their neighborhoods correlated with gene expression patterns
    in a predictable manner. This is central to understanding geometric control of
    genetic programs involved in cellular homeostasis and the associated diseases. '
author:
- first_name: Yejun
  full_name: Wang, Yejun
  last_name: Wang
- first_name: Mallika
  full_name: Nagarajan, Mallika
  last_name: Nagarajan
- first_name: Caroline
  full_name: Uhler, Caroline
  id: 49ADD78E-F248-11E8-B48F-1D18A9856A87
  last_name: Uhler
  orcid: 0000-0002-7008-0216
- first_name: Gv
  full_name: Shivashankar, Gv
  last_name: Shivashankar
citation:
  ama: Wang Y, Nagarajan M, Uhler C, Shivashankar G. Orientation and repositioning
    of chromosomes correlate with cell geometry dependent gene expression. <i>Molecular
    Biology of the Cell</i>. 2017;28(14):1997-2009. doi:<a href="https://doi.org/10.1091/mbc.E16-12-0825">10.1091/mbc.E16-12-0825</a>
  apa: Wang, Y., Nagarajan, M., Uhler, C., &#38; Shivashankar, G. (2017). Orientation
    and repositioning of chromosomes correlate with cell geometry dependent gene expression.
    <i>Molecular Biology of the Cell</i>. American Society for Cell Biology. <a href="https://doi.org/10.1091/mbc.E16-12-0825">https://doi.org/10.1091/mbc.E16-12-0825</a>
  chicago: Wang, Yejun, Mallika Nagarajan, Caroline Uhler, and Gv Shivashankar. “Orientation
    and Repositioning of Chromosomes Correlate with Cell Geometry Dependent Gene Expression.”
    <i>Molecular Biology of the Cell</i>. American Society for Cell Biology, 2017.
    <a href="https://doi.org/10.1091/mbc.E16-12-0825">https://doi.org/10.1091/mbc.E16-12-0825</a>.
  ieee: Y. Wang, M. Nagarajan, C. Uhler, and G. Shivashankar, “Orientation and repositioning
    of chromosomes correlate with cell geometry dependent gene expression,” <i>Molecular
    Biology of the Cell</i>, vol. 28, no. 14. American Society for Cell Biology, pp.
    1997–2009, 2017.
  ista: Wang Y, Nagarajan M, Uhler C, Shivashankar G. 2017. Orientation and repositioning
    of chromosomes correlate with cell geometry dependent gene expression. Molecular
    Biology of the Cell. 28(14), 1997–2009.
  mla: Wang, Yejun, et al. “Orientation and Repositioning of Chromosomes Correlate
    with Cell Geometry Dependent Gene Expression.” <i>Molecular Biology of the Cell</i>,
    vol. 28, no. 14, American Society for Cell Biology, 2017, pp. 1997–2009, doi:<a
    href="https://doi.org/10.1091/mbc.E16-12-0825">10.1091/mbc.E16-12-0825</a>.
  short: Y. Wang, M. Nagarajan, C. Uhler, G. Shivashankar, Molecular Biology of the
    Cell 28 (2017) 1997–2009.
date_created: 2018-12-11T11:47:59Z
date_published: 2017-07-07T00:00:00Z
date_updated: 2021-01-12T08:11:17Z
day: '07'
ddc:
- '519'
department:
- _id: CaUh
doi: 10.1091/mbc.E16-12-0825
file:
- access_level: open_access
  checksum: de01dac9e30970cfa6ae902480a4e04d
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:10:53Z
  date_updated: 2020-07-14T12:47:46Z
  file_id: '4844'
  file_name: IST-2017-892-v1+1_Mol._Biol._Cell-2017-Wang-1997-2009.pdf
  file_size: 1086097
  relation: main_file
file_date_updated: 2020-07-14T12:47:46Z
has_accepted_license: '1'
intvolume: '        28'
issue: '14'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-sa/4.0/
month: '07'
oa: 1
oa_version: Published Version
page: 1997 - 2009
project:
- _id: 2530CA10-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Y 903-N35
  name: 'Gaussian Graphical Models: Theory and Applications'
publication: Molecular Biology of the Cell
publication_identifier:
  issn:
  - '10591524'
publication_status: published
publisher: American Society for Cell Biology
publist_id: '7001'
pubrep_id: '892'
quality_controlled: '1'
scopus_import: 1
status: public
title: Orientation and repositioning of chromosomes correlate with cell geometry dependent
  gene expression
tmp:
  image: /images/cc_by_nc_sa.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
    BY-NC-SA 4.0)
  short: CC BY-NC-SA (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 28
year: '2017'
...