---
_id: '12203'
abstract:
- lang: eng
  text: 'Geranylgeranyl diphosphate synthase (GGPPS, EC: 2.5.1.29) catalyzes the biosynthesis
    of geranylgeranyl diphosphate (GGPP), which is a key precursor for ginkgolide
    biosynthesis. Here we reported for the first time the cloning of a new full-length
    cDNA encoding GGPPS from the living fossil plant Ginkgo biloba. The full-length
    cDNA encoding G. biloba GGPPS (designated as GbGGPPS) was 1657bp long and contained
    a 1176bp open reading frame encoding a 391 amino acid protein. Comparative analysis
    showed that GbGGPPS possessed a 79 amino acid transit peptide at its N-terminal,
    which directed GbGGPPS to target to the plastids. Bioinformatic analysis revealed
    that GbGGPPS was a member of polyprenyltransferases with two highly conserved
    aspartate-rich motifs like other plant GGPPSs. Phylogenetic tree analysis indicated
    that plant GGPPSs could be classified into two groups, angiosperm and gymnosperm
    GGPPSs, while GbGGPPS had closer relationship with gymnosperm plant GGPPSs.'
acknowledgement: This study was financially supported by China National High-Tech
  “863” Program. The authors are very thankful to Dr Li Wang (School of Life Sciences,
  Fudan University, Shanghai, China) for her kind help with constructing the phylogenetic
  tree.
article_processing_charge: No
article_type: original
author:
- first_name: Zhihua
  full_name: Liao, Zhihua
  last_name: Liao
- first_name: Min
  full_name: Chen, Min
  last_name: Chen
- first_name: Yifu
  full_name: Gong, Yifu
  last_name: Gong
- first_name: Liang
  full_name: Guo, Liang
  last_name: Guo
- first_name: Qiumin
  full_name: Tan, Qiumin
  last_name: Tan
- first_name: Xiaoqi
  full_name: Feng, Xiaoqi
  id: e0164712-22ee-11ed-b12a-d80fcdf35958
  last_name: Feng
  orcid: 0000-0002-4008-1234
- first_name: Xiaofen
  full_name: Sun, Xiaofen
  last_name: Sun
- first_name: Feng
  full_name: Tan, Feng
  last_name: Tan
- first_name: Kexuan
  full_name: Tang, Kexuan
  last_name: Tang
citation:
  ama: Liao Z, Chen M, Gong Y, et al. A new geranylgeranyl Diphosphate synthase gene
    from Ginkgo biloba, which intermediates the biosynthesis of the key precursor
    for ginkgolides. <i>DNA Sequence</i>. 2004;15(2):153-158. doi:<a href="https://doi.org/10.1080/10425170410001667348">10.1080/10425170410001667348</a>
  apa: Liao, Z., Chen, M., Gong, Y., Guo, L., Tan, Q., Feng, X., … Tang, K. (2004).
    A new geranylgeranyl Diphosphate synthase gene from Ginkgo biloba, which intermediates
    the biosynthesis of the key precursor for ginkgolides. <i>DNA Sequence</i>. Informa
    UK Limited. <a href="https://doi.org/10.1080/10425170410001667348">https://doi.org/10.1080/10425170410001667348</a>
  chicago: Liao, Zhihua, Min Chen, Yifu Gong, Liang Guo, Qiumin Tan, Xiaoqi Feng,
    Xiaofen Sun, Feng Tan, and Kexuan Tang. “A New Geranylgeranyl Diphosphate Synthase
    Gene from Ginkgo Biloba, Which Intermediates the Biosynthesis of the Key Precursor
    for Ginkgolides.” <i>DNA Sequence</i>. Informa UK Limited, 2004. <a href="https://doi.org/10.1080/10425170410001667348">https://doi.org/10.1080/10425170410001667348</a>.
  ieee: Z. Liao <i>et al.</i>, “A new geranylgeranyl Diphosphate synthase gene from
    Ginkgo biloba, which intermediates the biosynthesis of the key precursor for ginkgolides,”
    <i>DNA Sequence</i>, vol. 15, no. 2. Informa UK Limited, pp. 153–158, 2004.
  ista: Liao Z, Chen M, Gong Y, Guo L, Tan Q, Feng X, Sun X, Tan F, Tang K. 2004.
    A new geranylgeranyl Diphosphate synthase gene from Ginkgo biloba, which intermediates
    the biosynthesis of the key precursor for ginkgolides. DNA Sequence. 15(2), 153–158.
  mla: Liao, Zhihua, et al. “A New Geranylgeranyl Diphosphate Synthase Gene from Ginkgo
    Biloba, Which Intermediates the Biosynthesis of the Key Precursor for Ginkgolides.”
    <i>DNA Sequence</i>, vol. 15, no. 2, Informa UK Limited, 2004, pp. 153–58, doi:<a
    href="https://doi.org/10.1080/10425170410001667348">10.1080/10425170410001667348</a>.
  short: Z. Liao, M. Chen, Y. Gong, L. Guo, Q. Tan, X. Feng, X. Sun, F. Tan, K. Tang,
    DNA Sequence 15 (2004) 153–158.
date_created: 2023-01-16T09:24:50Z
date_published: 2004-01-01T00:00:00Z
date_updated: 2023-05-08T10:58:29Z
department:
- _id: XiFe
doi: 10.1080/10425170410001667348
extern: '1'
external_id:
  pmid:
  - '15352294'
intvolume: '        15'
issue: '2'
keyword:
- Endocrinology
- Genetics
- Molecular Biology
- Biochemistry
language:
- iso: eng
oa_version: None
page: 153-158
pmid: 1
publication: DNA Sequence
publication_identifier:
  issn:
  - 1042-5179
publication_status: published
publisher: Informa UK Limited
quality_controlled: '1'
scopus_import: '1'
status: public
title: A new geranylgeranyl Diphosphate synthase gene from Ginkgo biloba, which intermediates
  the biosynthesis of the key precursor for ginkgolides
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 15
year: '2004'
...