---
_id: '4146'
abstract:
- lang: eng
  text: During vertebrate gastrulation, highly coordinated cellular rearrangements
    lead to the formation of the three germ layers, ectoderm, mesoderm and endoderm.
    In zebrafish, silberblick (slb)/wnt11 regulates normal gastrulation movements
    by activating a signalling pathway similar to the Frizzled-signalling pathway,
    which establishes epithelial planar cell polarity (PCP) in Drosophila. However,
    the cellular mechanisms by which slb/wnt11 functions during zebrafish gastrulation
    are still unclear. Using high-resolution two-photon confocal imaging followed
    by computer-assisted reconstruction and motion analysis, we have analysed the
    movement and morphology of individual cells in three dimensions during the course
    of gastrulation. We show that in slb-mutant embryos, hypoblast cells within the
    forming germ ring have slower, less directed migratory movements at the onset
    of gastrulation. These aberrant cell movements are accompanied by defects in the
    orientation of cellular processes along the individual movement directions of
    these cells. We conclude that slb/wnt11-mediated orientation of cellular processes
    plays a role in facilitating and stabilising movements of hypoblast cells in the
    germ ring, thereby pointing at a novel function of the slb/wnt11 signalling pathway
    for the regulation of migratory cell movements at early stages of gastrulation.
acknowledgement: 'We thank Jennifer Geiger, Mathias Köppen, Christian Dahmann and
  Marcos Gonzalez-Gaitan for helpful comments on earlier versions of this manuscript,Beate
  Kilian for technical assistance, Ugur Yalcin, Katrin Anczok and Viktor Schnabel
  for help with the image analysis, Vinzenz Link for programming Excel Macros and
  Harald Brush-Janovjak for extensive reviews of the statistics part of this work.
  We are grateful to Kurt Anderson and Jan Peychl for help with the confocal microscopy.
  P.J.H., E.V. and D.R.S. are supported by NIH grant HD-18577, The W.M Keck Foundation
  and the Developmental Studies Hybridoma Bank(DSHB), P.J.H. by The American Cancer
  Society (grant # PF-01-110-01-CSM),M.L.C. and S.W.W. by the Wellcome Trust, M.T.
  by an MRC Career Development Award, and F.U. and C.P.H. by an Emmy-Noether-Fellowship
  from the DFG.'
article_processing_charge: No
article_type: original
author:
- first_name: Florian
  full_name: Ulrich, Florian
  last_name: Ulrich
- first_name: Miguel
  full_name: Concha, Miguel
  last_name: Concha
- first_name: Paul
  full_name: Heid, Paul
  last_name: Heid
- first_name: Ed
  full_name: Voss, Ed
  last_name: Voss
- first_name: Sabine
  full_name: Witzel, Sabine
  last_name: Witzel
- first_name: Henry
  full_name: Roehl, Henry
  last_name: Roehl
- first_name: Masazumi
  full_name: Tada, Masazumi
  last_name: Tada
- first_name: Stephen
  full_name: Wilson, Stephen
  last_name: Wilson
- first_name: Richard
  full_name: Adams, Richard
  last_name: Adams
- first_name: David
  full_name: Soll, David
  last_name: Soll
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Ulrich F, Concha M, Heid P, et al. Slb/Wnt11 controls hypoblast cell migration
    and morphogenesis at the onset of zebrafish gastrulation. <i>Development</i>.
    2003;130(22):5375-5384. doi:<a href="https://doi.org/10.1242/dev.00758">10.1242/dev.00758</a>
  apa: Ulrich, F., Concha, M., Heid, P., Voss, E., Witzel, S., Roehl, H., … Heisenberg,
    C.-P. J. (2003). Slb/Wnt11 controls hypoblast cell migration and morphogenesis
    at the onset of zebrafish gastrulation. <i>Development</i>. Company of Biologists.
    <a href="https://doi.org/10.1242/dev.00758">https://doi.org/10.1242/dev.00758</a>
  chicago: Ulrich, Florian, Miguel Concha, Paul Heid, Ed Voss, Sabine Witzel, Henry
    Roehl, Masazumi Tada, et al. “Slb/Wnt11 Controls Hypoblast Cell Migration and
    Morphogenesis at the Onset of Zebrafish Gastrulation.” <i>Development</i>. Company
    of Biologists, 2003. <a href="https://doi.org/10.1242/dev.00758">https://doi.org/10.1242/dev.00758</a>.
  ieee: F. Ulrich <i>et al.</i>, “Slb/Wnt11 controls hypoblast cell migration and
    morphogenesis at the onset of zebrafish gastrulation,” <i>Development</i>, vol.
    130, no. 22. Company of Biologists, pp. 5375–5384, 2003.
  ista: Ulrich F, Concha M, Heid P, Voss E, Witzel S, Roehl H, Tada M, Wilson S, Adams
    R, Soll D, Heisenberg C-PJ. 2003. Slb/Wnt11 controls hypoblast cell migration
    and morphogenesis at the onset of zebrafish gastrulation. Development. 130(22),
    5375–5384.
  mla: Ulrich, Florian, et al. “Slb/Wnt11 Controls Hypoblast Cell Migration and Morphogenesis
    at the Onset of Zebrafish Gastrulation.” <i>Development</i>, vol. 130, no. 22,
    Company of Biologists, 2003, pp. 5375–84, doi:<a href="https://doi.org/10.1242/dev.00758">10.1242/dev.00758</a>.
  short: F. Ulrich, M. Concha, P. Heid, E. Voss, S. Witzel, H. Roehl, M. Tada, S.
    Wilson, R. Adams, D. Soll, C.-P.J. Heisenberg, Development 130 (2003) 5375–5384.
date_created: 2018-12-11T12:07:13Z
date_published: 2003-11-15T00:00:00Z
date_updated: 2024-02-27T10:14:21Z
day: '15'
doi: 10.1242/dev.00758
extern: '1'
external_id:
  pmid:
  - ' PMC1414802'
intvolume: '       130'
issue: '22'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1414802/
month: '11'
oa: 1
oa_version: None
page: 5375 - 5384
pmid: 1
publication: Development
publication_identifier:
  issn:
  - 1011-6370
publication_status: published
publisher: Company of Biologists
publist_id: '1975'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Slb/Wnt11 controls hypoblast cell migration and morphogenesis at the onset
  of zebrafish gastrulation
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 130
year: '2003'
...