---
_id: '2622'
abstract:
- lang: eng
text: To understand the possible contribution of metabotropic γ-aminobutyric acid
receptors (GABABR) in cortical development, we investigated the expression pattern
and the cellular and subcellular localization of the GABABR1 and GABABR2 subtypes
in the rat neocortex from embryonic day 14 (E14) to adulthood. At the light microscopic
level, both GABABR1 and GABABR2 were detected as early as E14. During prenatal
development, both subtypes were expressed highly in the cortical plate. Using
double immunofluorescence, GABABR1 colocalized with GABABR2 in neurons of the
marginal zone and subplate, indicating that these proteins are coexpressed and
could be forming functional GABABRs during prenatal development in vivo. In contrast,
only GABABR1 but not GABABR2 was detected in the tangentially migratory cells
in the lower intermediate zone. During postnatal development, immunoreactivity
for GABABR1 and GABABR2 was distributed mainly in pyramidal cells. Discrete GABABR1-immunopositive
cell bodies of interneurons were present throughout the neocortex. In addition,
GABABR1 but not GABABR2 was found in identified Cajal-Retzius cells in layer I.
At the electron microscopic level, immunoreactivity for GABABR1 and GABABR2 was
found in dendritic spines and dendritic shafts at extrasynaptic and perisynaptic
sites throughout postnatal development. We further demonstrated the presynaptic
localization of GABABR1 and GABABR2, as well as the association of the receptors
with asymmetrical synaptic junctions. These results indicate potentially important
roles for the GABABRs in the regulation of migratory processes during corticogenesis
and in the modulation of synaptic transmission during early development of cortical
circuitry.
acknowledgement: The authors are grateful to Dr Marco Sassoe-Pogneto for his comments
on a previous version of the manuscript. We also would like to thank to Ms. Courtney
Voelker for the English revision and comments of the manuscript. This work was made
possible by grants from the European Community (QLG3-CT-1999–00192, R.L) and the
Spanish Ministry of Science and Technology (PB97-0582-CO2-01, A.F).
article_processing_charge: No
article_type: original
author:
- first_name: Guillermina
full_name: López Bendito, Guillermina
last_name: López Bendito
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Ákos
full_name: Kulik, Ákos
last_name: Kulik
- first_name: Ole
full_name: Paulsen, Ole
last_name: Paulsen
- first_name: Alfonso
full_name: Fairén, Alfonso
last_name: Fairén
- first_name: Rafael
full_name: Luján, Rafael
last_name: Luján
citation:
ama: López Bendito G, Shigemoto R, Kulik Á, Paulsen O, Fairén A, Luján R. Expression
and distribution of metabotropic GABA receptor subtypes GABABR1 and GABABR2 during
rat neocortical development. European Journal of Neuroscience. 2002;15(11):1766-1778.
doi:10.1046/j.1460-9568.2002.02032.x
apa: López Bendito, G., Shigemoto, R., Kulik, Á., Paulsen, O., Fairén, A., &
Luján, R. (2002). Expression and distribution of metabotropic GABA receptor subtypes
GABABR1 and GABABR2 during rat neocortical development. European Journal of
Neuroscience. Wiley-Blackwell. https://doi.org/10.1046/j.1460-9568.2002.02032.x
chicago: López Bendito, Guillermina, Ryuichi Shigemoto, Ákos Kulik, Ole Paulsen,
Alfonso Fairén, and Rafael Luján. “Expression and Distribution of Metabotropic
GABA Receptor Subtypes GABABR1 and GABABR2 during Rat Neocortical Development.”
European Journal of Neuroscience. Wiley-Blackwell, 2002. https://doi.org/10.1046/j.1460-9568.2002.02032.x.
ieee: G. López Bendito, R. Shigemoto, Á. Kulik, O. Paulsen, A. Fairén, and R. Luján,
“Expression and distribution of metabotropic GABA receptor subtypes GABABR1 and
GABABR2 during rat neocortical development,” European Journal of Neuroscience,
vol. 15, no. 11. Wiley-Blackwell, pp. 1766–1778, 2002.
ista: López Bendito G, Shigemoto R, Kulik Á, Paulsen O, Fairén A, Luján R. 2002.
Expression and distribution of metabotropic GABA receptor subtypes GABABR1 and
GABABR2 during rat neocortical development. European Journal of Neuroscience.
15(11), 1766–1778.
mla: López Bendito, Guillermina, et al. “Expression and Distribution of Metabotropic
GABA Receptor Subtypes GABABR1 and GABABR2 during Rat Neocortical Development.”
European Journal of Neuroscience, vol. 15, no. 11, Wiley-Blackwell, 2002,
pp. 1766–78, doi:10.1046/j.1460-9568.2002.02032.x.
short: G. López Bendito, R. Shigemoto, Á. Kulik, O. Paulsen, A. Fairén, R. Luján,
European Journal of Neuroscience 15 (2002) 1766–1778.
date_created: 2018-12-11T11:58:43Z
date_published: 2002-06-01T00:00:00Z
date_updated: 2023-07-19T07:30:39Z
day: '01'
doi: 10.1046/j.1460-9568.2002.02032.x
extern: '1'
external_id:
pmid:
- '12081656'
intvolume: ' 15'
issue: '11'
language:
- iso: eng
month: '06'
oa_version: None
page: 1766 - 1778
pmid: 1
publication: European Journal of Neuroscience
publication_identifier:
issn:
- 0953-816X
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4276'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Expression and distribution of metabotropic GABA receptor subtypes GABABR1
and GABABR2 during rat neocortical development
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 15
year: '2002'
...
---
_id: '2624'
abstract:
- lang: eng
text: Metabotropic γ-aminobutyric acid receptors (GABABRs) are involved in modulation
of synaptic transmission and activity of cerebellar and thalamic neurons. We used
subtype-specific antibodies in pre- and postembedding immunohistochemistry combined
with three-dimensional reconstruction of labelled profiles and quantification
of immunoparticles to reveal the subcellular distribution of pre- and postsynaptic
GABABR1a/b and GABABR2 in the rat cerebellum and ventrobasal thalamus. GABABR1a/b
and R2 were extensively colocalized in most brain regions including the cerebellum
and thalamus. In the cerebellum, immunoreactivity for both subtypes was prevalent
in the molecular layer. The most intense immunoreactivity was found in Purkinje
cell spines with a high density of immunoparticles at extrasynaptic sites peaking
at around 240 nm from glutamatergic synapses between spines and parallel fibre
varicosities. This is in contrast to dendrites at sites around GABAergic synapses
where sparse and random distribution was found for both subtypes. In addition,
more than one-tenth of the synaptic membrane specialization of spine-parallel
fibre synapses were labelled at pre- or postsynaptic sites. Weak immunolabelling
for both subtypes was also seen in parallel fibres but only rarely in GABAergic
axons. In the ventrobasal thalamus, immunolabelling for both receptor subtypes
was intense over the dendritic field of thalamocortical cells. Electron microscopy
demonstrated an extrasynaptic localization of GABABR1a/b and R2 exclusively in
postsynaptic elements. Quantitative analysis further revealed the density of GABABR1a/b
around GABAergic synapses was higher than glutamatergic synapses on thalamocortical
cell dendrites. The distinct localization of GABABRs relative to synaptic sites
in the cerebellum and ventrobasal thalamus suggests that GABABRs differentially
regulate activity of different neuronal populations.
acknowledgement: This work was supported by research grants from the Ministry of Education,
Science, Sports and Culture of Japan, and the Japan Society for the Promotion of
Science (P96319). We thank Drs L. Zaborszky and R. Luján for their comments on the
manuscript, Dr M. Watanabe for kindly supplying us with GluRδ2 and AMPA GluR1 antibodies,
Dr R.E. Edwards for rabbit BNPI antibody, and J. Hatakeyama and S. Doi for technical
assistance.
article_processing_charge: No
article_type: original
author:
- first_name: Ákos
full_name: Kulik, Ákos
last_name: Kulik
- first_name: Kazuhiko
full_name: Nakadate, Kazuhiko
last_name: Nakadate
- first_name: Gábor
full_name: Nyíri, Gábor
last_name: Nyíri
- first_name: Takuya
full_name: Notomi, Takuya
last_name: Notomi
- first_name: Barbara
full_name: Malitschek, Barbara
last_name: Malitschek
- first_name: Bernhard
full_name: Bettler, Bernhard
last_name: Bettler
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Kulik Á, Nakadate K, Nyíri G, et al. Distinct localization of GABAB receptors
relative to synaptic sites in the rat cerebellum and ventrobasal thalamus. European
Journal of Neuroscience. 2002;15(2):291-307. doi:10.1046/j.0953-816x.2001.01855.x
apa: Kulik, Á., Nakadate, K., Nyíri, G., Notomi, T., Malitschek, B., Bettler, B.,
& Shigemoto, R. (2002). Distinct localization of GABAB receptors relative
to synaptic sites in the rat cerebellum and ventrobasal thalamus. European
Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1046/j.0953-816x.2001.01855.x
chicago: Kulik, Ákos, Kazuhiko Nakadate, Gábor Nyíri, Takuya Notomi, Barbara Malitschek,
Bernhard Bettler, and Ryuichi Shigemoto. “Distinct Localization of GABAB Receptors
Relative to Synaptic Sites in the Rat Cerebellum and Ventrobasal Thalamus.” European
Journal of Neuroscience. Wiley-Blackwell, 2002. https://doi.org/10.1046/j.0953-816x.2001.01855.x.
ieee: Á. Kulik et al., “Distinct localization of GABAB receptors relative
to synaptic sites in the rat cerebellum and ventrobasal thalamus,” European
Journal of Neuroscience, vol. 15, no. 2. Wiley-Blackwell, pp. 291–307, 2002.
ista: Kulik Á, Nakadate K, Nyíri G, Notomi T, Malitschek B, Bettler B, Shigemoto
R. 2002. Distinct localization of GABAB receptors relative to synaptic sites in
the rat cerebellum and ventrobasal thalamus. European Journal of Neuroscience.
15(2), 291–307.
mla: Kulik, Ákos, et al. “Distinct Localization of GABAB Receptors Relative to Synaptic
Sites in the Rat Cerebellum and Ventrobasal Thalamus.” European Journal of
Neuroscience, vol. 15, no. 2, Wiley-Blackwell, 2002, pp. 291–307, doi:10.1046/j.0953-816x.2001.01855.x.
short: Á. Kulik, K. Nakadate, G. Nyíri, T. Notomi, B. Malitschek, B. Bettler, R.
Shigemoto, European Journal of Neuroscience 15 (2002) 291–307.
date_created: 2018-12-11T11:58:44Z
date_published: 2002-01-01T00:00:00Z
date_updated: 2023-07-18T13:08:40Z
day: '01'
doi: 10.1046/j.0953-816x.2001.01855.x
extern: '1'
external_id:
pmid:
- '11849296'
intvolume: ' 15'
issue: '2'
language:
- iso: eng
month: '01'
oa_version: None
page: 291 - 307
pmid: 1
publication: European Journal of Neuroscience
publication_identifier:
issn:
- 0953-816X
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4275'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distinct localization of GABAB receptors relative to synaptic sites in the
rat cerebellum and ventrobasal thalamus
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 15
year: '2002'
...
---
_id: '2606'
abstract:
- lang: eng
text: Glutamate receptors have been linked to the regulation of several developmental
events in the CNS. By using cortical slices of early postnatal mice, we show that
in layer I cells, glutamate produces intracellular calcium ([Ca2+]i) elevations
mediated by ionotropic and metabotropic glutamate receptors (mGluRs). The contribution
of mGluRs to these responses was demonstrated by application of tACPD, an agonist
to groups I and II mGluRs, which evoked [Ca2+]i increases that could be reversibly
blocked by MCPG, an antagonist to groups I and II mGluRs. In the absence of extracellular
Ca2+, repetitive applications of tACPD or quisqualate, an agonist to group I mGluRs,
elicited decreasing [Ca2+]i responses that were restored by refilling a thapsigargin-sensitive
Ca2+ store. The use of specific group I mGluR agonists CHPG and DHPG indicated
that the functional mGluR in layer I was of the mGluR1 subtype. Subtype specific
antibodies confirmed the presence of mGlur1α, but not mGluR5, in Cajal-Retzius
(Reelin-immunoreactive) neurons.
acknowledgement: MV and AF are senior coauthors of this work, which was supported by
Ministerio de Educacion y Cultura, grants SAF97/0195 and SAF 2000-0152-C02-02 to
M.V; PB94-0219-CO2-01 and PB97-0582-CO2-01 to A.F., Accio Especial de R+D AE98-18 from Generalitat Valenciana, and a Fellowship
from Bancaixa-C.S.I.C. to J.R.M.-G. We wish to thank Andre M. Goffinet for his G10 antireelin antibody and Roberto Gallego, Juan M. Luque and Felix
Viana for their constructive criticisms on previous versions of the manuscript.
article_processing_charge: No
article_type: original
author:
- first_name: Galán
full_name: Martínez, Galán
last_name: Martínez
- first_name: Guillermina
full_name: López Bendito, Guillermina
last_name: López Bendito
- first_name: Rafael
full_name: Luján, Rafael
last_name: Luján
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Alfonso
full_name: Fairén, Alfonso
last_name: Fairén
- first_name: Miguel
full_name: Valdeolmillos, Miguel
last_name: Valdeolmillos
citation:
ama: Martínez G, López Bendito G, Luján R, Shigemoto R, Fairén A, Valdeolmillos
M. Cajal-Retzius cells in early postnatal mouse cortex selectively express functional
metabotropic glutamate receptors. European Journal of Neuroscience. 2001;13(6):1147-1154.
doi:10.1046/j.0953-816X.2001.01494.x
apa: Martínez, G., López Bendito, G., Luján, R., Shigemoto, R., Fairén, A., &
Valdeolmillos, M. (2001). Cajal-Retzius cells in early postnatal mouse cortex
selectively express functional metabotropic glutamate receptors. European Journal
of Neuroscience. Wiley-Blackwell. https://doi.org/10.1046/j.0953-816X.2001.01494.x
chicago: Martínez, Galán, Guillermina López Bendito, Rafael Luján, Ryuichi Shigemoto,
Alfonso Fairén, and Miguel Valdeolmillos. “Cajal-Retzius Cells in Early Postnatal
Mouse Cortex Selectively Express Functional Metabotropic Glutamate Receptors.”
European Journal of Neuroscience. Wiley-Blackwell, 2001. https://doi.org/10.1046/j.0953-816X.2001.01494.x.
ieee: G. Martínez, G. López Bendito, R. Luján, R. Shigemoto, A. Fairén, and M. Valdeolmillos,
“Cajal-Retzius cells in early postnatal mouse cortex selectively express functional
metabotropic glutamate receptors,” European Journal of Neuroscience, vol.
13, no. 6. Wiley-Blackwell, pp. 1147–1154, 2001.
ista: Martínez G, López Bendito G, Luján R, Shigemoto R, Fairén A, Valdeolmillos
M. 2001. Cajal-Retzius cells in early postnatal mouse cortex selectively express
functional metabotropic glutamate receptors. European Journal of Neuroscience.
13(6), 1147–1154.
mla: Martínez, Galán, et al. “Cajal-Retzius Cells in Early Postnatal Mouse Cortex
Selectively Express Functional Metabotropic Glutamate Receptors.” European
Journal of Neuroscience, vol. 13, no. 6, Wiley-Blackwell, 2001, pp. 1147–54,
doi:10.1046/j.0953-816X.2001.01494.x.
short: G. Martínez, G. López Bendito, R. Luján, R. Shigemoto, A. Fairén, M. Valdeolmillos,
European Journal of Neuroscience 13 (2001) 1147–1154.
date_created: 2018-12-11T11:58:38Z
date_published: 2001-03-01T00:00:00Z
date_updated: 2023-05-24T12:53:46Z
day: '01'
doi: 10.1046/j.0953-816X.2001.01494.x
extern: '1'
external_id:
pmid:
- '11285012'
intvolume: ' 13'
issue: '6'
language:
- iso: eng
month: '03'
oa_version: None
page: 1147 - 1154
pmid: 1
publication: European Journal of Neuroscience
publication_identifier:
issn:
- 0953-816X
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4293'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cajal-Retzius cells in early postnatal mouse cortex selectively express functional
metabotropic glutamate receptors
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 13
year: '2001'
...
---
_id: '3519'
abstract:
- lang: eng
text: 'In contrast to sensory cortical areas of the brain, the relevant physiological
inputs to the hippocampus, leading to selective activation of pyramidal cells,
are largely unknown. Pyramidal cells are thought to be phasically activated by
spatial cues and a variety of sensory and motor stimuli. Here, we used a behavioural
`space clamp'' method, which involved the confinement of the actively running
animal in a defined position in space (running wheel) and kept sensory inputs
constant. Twelve percent of the recorded CA1 pyramidal cells were selectively
active while the rat was running in the wheel. Cell firing was specific to the
direction of running and disappeared after rotating the recording apparatus. The
discharge frequency of pyramidal cells and interneurons was sustained as long
as the rat ran continuously in the wheel. Furthermore, the discharge frequency
of pyramidal cells and interneurons increased with increasing running velocity,
even though the frequency of hippocampal theta waves remained constant. The discharge
frequency of some `wheel-related'' pyramidal cells could increase more than 10-fold
between 10 and 100 cm/s, whereas the firing rate of `non-wheel'' cells remained
constantly low. We hypothesize that: (i) a necessary condition for place-specific
discharge of hippocampal pyramidal cells is the presence of theta oscillation;
and (ii) relevant stimuli can tonically and selectively activate hippocampal pyramidal
cells as long as theta activity is present.'
acknowledgement: We thank M. Recce for continuous support, A. Berthoz for advice,
K. Moorefor his participation in the early stages of the experiments, J. Lee for helpand
C. King for his comments on the manuscript. This work was supportedby NIH (NS34994,
MH54671), the Human Frontier Science Program (H.H.),the Hungarian Eo ̈tvo ̈s State
Fellowship (A.C.) and the Soros Foundation (A.C.)
article_processing_charge: No
article_type: original
author:
- first_name: András
full_name: Czurkó, András
last_name: Czurkó
- first_name: Hajima
full_name: Hirase, Hajima
last_name: Hirase
- first_name: Jozsef L
full_name: Csicsvari, Jozsef L
id: 3FA14672-F248-11E8-B48F-1D18A9856A87
last_name: Csicsvari
orcid: 0000-0002-5193-4036
- first_name: György
full_name: Buzsáki, György
last_name: Buzsáki
citation:
ama: Czurkó A, Hirase H, Csicsvari JL, Buzsáki G. Sustained activation of hippocampal
pyramidal cells by ‘space clamping’’ in a running wheel.’ European Journal
of Neuroscience. 1999;11(1):344-352. doi:10.1046/j.1460-9568.1999.00446.x
apa: Czurkó, A., Hirase, H., Csicsvari, J. L., & Buzsáki, G. (1999). Sustained
activation of hippocampal pyramidal cells by ‘space clamping’’ in a running wheel.’
European Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1046/j.1460-9568.1999.00446.x
chicago: Czurkó, András, Hajima Hirase, Jozsef L Csicsvari, and György Buzsáki.
“Sustained Activation of Hippocampal Pyramidal Cells by ‘space Clamping’’ in a
Running Wheel.’” European Journal of Neuroscience. Wiley-Blackwell, 1999.
https://doi.org/10.1046/j.1460-9568.1999.00446.x.
ieee: A. Czurkó, H. Hirase, J. L. Csicsvari, and G. Buzsáki, “Sustained activation
of hippocampal pyramidal cells by ‘space clamping’’ in a running wheel,’” European
Journal of Neuroscience, vol. 11, no. 1. Wiley-Blackwell, pp. 344–352, 1999.
ista: Czurkó A, Hirase H, Csicsvari JL, Buzsáki G. 1999. Sustained activation of
hippocampal pyramidal cells by ‘space clamping’’ in a running wheel’. European
Journal of Neuroscience. 11(1), 344–352.
mla: Czurkó, András, et al. “Sustained Activation of Hippocampal Pyramidal Cells
by ‘space Clamping’’ in a Running Wheel.’” European Journal of Neuroscience,
vol. 11, no. 1, Wiley-Blackwell, 1999, pp. 344–52, doi:10.1046/j.1460-9568.1999.00446.x.
short: A. Czurkó, H. Hirase, J.L. Csicsvari, G. Buzsáki, European Journal of Neuroscience
11 (1999) 344–352.
date_created: 2018-12-11T12:03:45Z
date_published: 1999-01-01T00:00:00Z
date_updated: 2022-09-07T13:09:08Z
day: '01'
doi: 10.1046/j.1460-9568.1999.00446.x
extern: '1'
external_id:
pmid:
- '9987037'
intvolume: ' 11'
issue: '1'
language:
- iso: eng
month: '01'
oa_version: None
page: 344 - 352
pmid: 1
publication: European Journal of Neuroscience
publication_identifier:
issn:
- 0953-816X
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2867'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Sustained activation of hippocampal pyramidal cells by ‘space clamping' in
a running wheel
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 11
year: '1999'
...
---
_id: '3539'
abstract:
- lang: eng
text: In the hippocampus, spatial representation of the environment has been suggested
to be coded by either the firing rate of pyramidal cell assemblies or the relative
timing of the action potentials during the theta EEG cycle. Here, we used a behavioural
`space clamp' method, which involved the confinement of the actively running animal
in a defined position in space (running wheel) to examine how `spatial' and other
inputs affect firing rate and timing of hippocampal CA1 pyramidal cells and interneurons.
Nineteen per cent of the recorded CA1 pyramidal cells were selectively active
while the rat was running in the wheel in a given direction ('wheel' cells). Spatial
rotation of the apparatus showed that selective discharge of pyramidal cells in
the wheel was under the combined influence of distal and apparatus cues. During
steady running, both discharge rate and theta phase were constant. Rotation of
the wheel apparatus resulted in a shift of both firing rate and preferred theta
phase. The discharge frequency of `wheel' cells increased threefold (on average)
with increasing running velocity. In contrast, change in running speed had relatively
little effect on the theta phase-related discharge of `wheel' cells. Our findings
indicate that mechanisms that regulate rate and phase of spikes are overlapping
but not necessarily identical.
acknowledgement: 'We thank M. Recce for his comments on the manuscript. This work
wassupported by NIH (NS34994, MH54671), the Human Frontier ScienceProgram (H.H.),
the EoÈtvoÈs State Fellowship (A.C.) and the Soros Foundation (A.C.) '
article_processing_charge: No
article_type: original
author:
- first_name: Hajima
full_name: Hirase, Hajima
last_name: Hirase
- first_name: András
full_name: Czurkó, András
last_name: Czurkó
- first_name: Jozsef L
full_name: Csicsvari, Jozsef L
id: 3FA14672-F248-11E8-B48F-1D18A9856A87
last_name: Csicsvari
orcid: 0000-0002-5193-4036
- first_name: György
full_name: Buzsáki, György
last_name: Buzsáki
citation:
ama: Hirase H, Czurkó A, Csicsvari JL, Buzsáki G. Firing rate and theta-phase coding
by hippocampal pyramidal neurons during ‘space clamping.’ European Journal
of Neuroscience. 1999;11(12):4373-4380. doi:10.1046/j.1460-9568.1999.00853.x
apa: Hirase, H., Czurkó, A., Csicsvari, J. L., & Buzsáki, G. (1999). Firing
rate and theta-phase coding by hippocampal pyramidal neurons during ‘space clamping.’
European Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1046/j.1460-9568.1999.00853.x
chicago: Hirase, Hajima, András Czurkó, Jozsef L Csicsvari, and György Buzsáki.
“Firing Rate and Theta-Phase Coding by Hippocampal Pyramidal Neurons during ‘Space
Clamping.’” European Journal of Neuroscience. Wiley-Blackwell, 1999. https://doi.org/10.1046/j.1460-9568.1999.00853.x.
ieee: H. Hirase, A. Czurkó, J. L. Csicsvari, and G. Buzsáki, “Firing rate and theta-phase
coding by hippocampal pyramidal neurons during ‘space clamping,’” European
Journal of Neuroscience, vol. 11, no. 12. Wiley-Blackwell, pp. 4373–4380,
1999.
ista: Hirase H, Czurkó A, Csicsvari JL, Buzsáki G. 1999. Firing rate and theta-phase
coding by hippocampal pyramidal neurons during ‘space clamping’. European Journal
of Neuroscience. 11(12), 4373–4380.
mla: Hirase, Hajima, et al. “Firing Rate and Theta-Phase Coding by Hippocampal Pyramidal
Neurons during ‘Space Clamping.’” European Journal of Neuroscience, vol.
11, no. 12, Wiley-Blackwell, 1999, pp. 4373–80, doi:10.1046/j.1460-9568.1999.00853.x.
short: H. Hirase, A. Czurkó, J.L. Csicsvari, G. Buzsáki, European Journal of Neuroscience
11 (1999) 4373–4380.
date_created: 2018-12-11T12:03:51Z
date_published: 1999-12-01T00:00:00Z
date_updated: 2022-09-06T09:45:36Z
day: '01'
doi: 10.1046/j.1460-9568.1999.00853.x
extern: '1'
external_id:
pmid:
- '10594664 '
intvolume: ' 11'
issue: '12'
language:
- iso: eng
month: '12'
oa_version: None
page: 4373 - 4380
pmid: 1
publication: European Journal of Neuroscience
publication_identifier:
issn:
- 0953-816X
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2845'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Firing rate and theta-phase coding by hippocampal pyramidal neurons during
‘space clamping’
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 11
year: '1999'
...
---
_id: '2574'
abstract:
- lang: eng
text: lonotropic and metabotropic (mGluR1a) glutamate receptors were reported to
be segregated from each other within the postsynaptic membrane at individual synapses.
In order to establish whether this pattern of distribution applies to the hippocampal
principal cells and to other postsynaptic metabotropic glutamate receptors, the
mGluR1a/b/c and mGluR5 subtypes were localized by immunocytochemistry. Principal
cells in all hippocampal fields were reactive for mGluR5, the strata oriens and
radiatum of the CA1 area being most strongly immunolabelled. Labelling for mGluR1b/c
was strongest on some pyramids in the CA3 area, weaker on granule cells and absent
on CA1 pyramids. Subpopulations of non-principal cells showed strong mGluR1 or
mGluR5 immunoreactivity. Electron microscopic pre-embedding immunoperoxidase and
both pre- and postembedding immunogold methods consistently revealed the extrasynaptic
location of both mGluRs in the somatic and dendritic membrane of pyramidal and
granule cells. The density of immunolabelling was highest on dendritic spines.
At synapses, immunoparticles for both mGluR1 and mGluR5 were found always outside
the postsynaptic membrane specializations. Receptors were particularly concentrated
in a perisynaptic annulus around type 1 synaptic junctions, including the invaginations
at 'perforated' synapses. Measurements of immunolabelling on dendritic spines
showed decreasing levels of receptor as a function of distance from the edge of
the synaptic specialization. We propose that glutamatergic synapses with an irregular
edge develop in order to increase the circumference of synaptic junctions leading
to an increase in the metabotropic to ionotropic glutamate receptor ratio at glutamate
release sites. The perisynaptic position of postsynaptic metabotropic glutamate
receptors appears to be a general feature of glutamatergic synaptic organization
and may apply to other G-protein-coupled receptors. © European Neuroscience Association.
article_processing_charge: No
article_type: original
author:
- first_name: Rafael
full_name: Luján, Rafael
last_name: Luján
- first_name: Zoltán
full_name: Nusser, Zoltán
last_name: Nusser
- first_name: John
full_name: Roberts, John
last_name: Roberts
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Péter
full_name: Somogyi, Péter
last_name: Somogyi
citation:
ama: Luján R, Nusser Z, Roberts J, Shigemoto R, Somogyi P. Perisynaptic location
of metabotropic glutamate receptors mGluR1 and mGluR5 on dendrites and dendritic
spines in the rat hippocampus. European Journal of Neuroscience. 1996;8(7):1488-1500.
doi:10.1111/j.1460-9568.1996.tb01611.x
apa: Luján, R., Nusser, Z., Roberts, J., Shigemoto, R., & Somogyi, P. (1996). Perisynaptic
location of metabotropic glutamate receptors mGluR1 and mGluR5 on dendrites and
dendritic spines in the rat hippocampus. European Journal of Neuroscience.
Wiley-Blackwell. https://doi.org/10.1111/j.1460-9568.1996.tb01611.x
chicago: Luján, Rafael, Zoltán Nusser, John Roberts, Ryuichi Shigemoto, and Péter
Somogyi. “ Perisynaptic Location of Metabotropic Glutamate Receptors MGluR1 and
MGluR5 on Dendrites and Dendritic Spines in the Rat Hippocampus.” European
Journal of Neuroscience. Wiley-Blackwell, 1996. https://doi.org/10.1111/j.1460-9568.1996.tb01611.x.
ieee: R. Luján, Z. Nusser, J. Roberts, R. Shigemoto, and P. Somogyi, “ Perisynaptic
location of metabotropic glutamate receptors mGluR1 and mGluR5 on dendrites and
dendritic spines in the rat hippocampus,” European Journal of Neuroscience,
vol. 8, no. 7. Wiley-Blackwell, pp. 1488–1500, 1996.
ista: Luján R, Nusser Z, Roberts J, Shigemoto R, Somogyi P. 1996. Perisynaptic
location of metabotropic glutamate receptors mGluR1 and mGluR5 on dendrites and
dendritic spines in the rat hippocampus. European Journal of Neuroscience. 8(7),
1488–1500.
mla: Luján, Rafael, et al. “ Perisynaptic Location of Metabotropic Glutamate Receptors
MGluR1 and MGluR5 on Dendrites and Dendritic Spines in the Rat Hippocampus.” European
Journal of Neuroscience, vol. 8, no. 7, Wiley-Blackwell, 1996, pp. 1488–500,
doi:10.1111/j.1460-9568.1996.tb01611.x.
short: R. Luján, Z. Nusser, J. Roberts, R. Shigemoto, P. Somogyi, European Journal
of Neuroscience 8 (1996) 1488–1500.
date_created: 2018-12-11T11:58:28Z
date_published: 1996-07-01T00:00:00Z
date_updated: 2022-08-11T11:57:08Z
day: '01'
doi: 10.1111/j.1460-9568.1996.tb01611.x
extern: '1'
external_id:
pmid:
- '8758956 '
intvolume: ' 8'
issue: '7'
language:
- iso: eng
month: '07'
oa_version: None
page: 1488 - 1500
pmid: 1
publication: European Journal of Neuroscience
publication_identifier:
issn:
- 0953-816X
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4324'
quality_controlled: '1'
scopus_import: '1'
status: public
title: ' Perisynaptic location of metabotropic glutamate receptors mGluR1 and mGluR5
on dendrites and dendritic spines in the rat hippocampus'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 8
year: '1996'
...
---
_id: '4175'
abstract:
- lang: eng
text: We have studied the effects of different neurotrophins on the survival and
proliferation of rat cerebellar granule cells in culture. These neurons express
trkB and trkC, the putative neuronal receptors for brain-derived neurotrophic
factor (BDNF) and neurotrophin-3 (NT-3) respectively. Binding studies using iodinated
BDNF and NT-3 demonstrated that both BDNF and NT-3 bind to the cerebellar granule
neurons with a similar affinity of approximately 2 x 10(-9) M. The number of receptors
per granule cell was surprisingly high, approximately 30 x 10(-4) and 2 x 10(5)
for BDNF and NT-3, respectively. Both NT-3 and BDNF elevated c-fos mRNA in the
granule neurons, but only BDNF up-regulated the mRNA encoding the low-affinity
neurotrophin receptor (p75). In contrast to NT-3, BDNF acted as a survival factor
for the granule neurons. BDNF also induced sprouting of the granule neurons and
significantly protected them against neurotoxicity induced by high (1 mM) glutamate
concentrations. Cultured granule neurons also expressed low levels of BDNF mRNA
which were increased by kainic acid, a glutamate receptor agonist. Thus, BDNF,
but not NT-3, is a survival factor for cultured cerebellar granule neurons and
activation of glutamate receptor(s) up-regulates BDNF expression in these cells.
article_processing_charge: No
article_type: original
author:
- first_name: Dan
full_name: Lindholm, Dan
last_name: Lindholm
- first_name: Georg
full_name: Dechant, Georg
last_name: Dechant
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Hans
full_name: Thoenen, Hans
last_name: Thoenen
citation:
ama: Lindholm D, Dechant G, Heisenberg C-PJ, Thoenen H. Brain-derived neurotrophic
factor is a survival factor for cultured rat cerebellar granule neurons and protects
them against glutamate-induced neurotoxicity. European Journal of Neuroscience.
1993;5(11):1455-1464. doi:10.1111/j.1460-9568.1993.tb00213.x
apa: Lindholm, D., Dechant, G., Heisenberg, C.-P. J., & Thoenen, H. (1993).
Brain-derived neurotrophic factor is a survival factor for cultured rat cerebellar
granule neurons and protects them against glutamate-induced neurotoxicity. European
Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1111/j.1460-9568.1993.tb00213.x
chicago: Lindholm, Dan, Georg Dechant, Carl-Philipp J Heisenberg, and Hans Thoenen.
“Brain-Derived Neurotrophic Factor Is a Survival Factor for Cultured Rat Cerebellar
Granule Neurons and Protects Them against Glutamate-Induced Neurotoxicity.” European
Journal of Neuroscience. Wiley-Blackwell, 1993. https://doi.org/10.1111/j.1460-9568.1993.tb00213.x.
ieee: D. Lindholm, G. Dechant, C.-P. J. Heisenberg, and H. Thoenen, “Brain-derived
neurotrophic factor is a survival factor for cultured rat cerebellar granule neurons
and protects them against glutamate-induced neurotoxicity,” European Journal
of Neuroscience, vol. 5, no. 11. Wiley-Blackwell, pp. 1455–1464, 1993.
ista: Lindholm D, Dechant G, Heisenberg C-PJ, Thoenen H. 1993. Brain-derived neurotrophic
factor is a survival factor for cultured rat cerebellar granule neurons and protects
them against glutamate-induced neurotoxicity. European Journal of Neuroscience.
5(11), 1455–1464.
mla: Lindholm, Dan, et al. “Brain-Derived Neurotrophic Factor Is a Survival Factor
for Cultured Rat Cerebellar Granule Neurons and Protects Them against Glutamate-Induced
Neurotoxicity.” European Journal of Neuroscience, vol. 5, no. 11, Wiley-Blackwell,
1993, pp. 1455–64, doi:10.1111/j.1460-9568.1993.tb00213.x.
short: D. Lindholm, G. Dechant, C.-P.J. Heisenberg, H. Thoenen, European Journal
of Neuroscience 5 (1993) 1455–1464.
date_created: 2018-12-11T12:07:24Z
date_published: 1993-11-01T00:00:00Z
date_updated: 2022-03-28T13:33:18Z
day: '01'
doi: 10.1111/j.1460-9568.1993.tb00213.x
extern: '1'
external_id:
pmid:
- '7904521 '
intvolume: ' 5'
issue: '11'
language:
- iso: eng
main_file_link:
- url: https://onlinelibrary.wiley.com/doi/10.1111/j.1460-9568.1993.tb00213.x
month: '11'
oa_version: None
page: 1455 - 1464
pmid: 1
publication: European Journal of Neuroscience
publication_identifier:
issn:
- 0953-816X
publication_status: published
publisher: Wiley-Blackwell
publist_id: '1943'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Brain-derived neurotrophic factor is a survival factor for cultured rat cerebellar
granule neurons and protects them against glutamate-induced neurotoxicity
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 5
year: '1993'
...