---
_id: '676'
abstract:
- lang: eng
  text: The segregation of different cell types into distinct tissues is a fundamental
    process in metazoan development. Differences in cell adhesion and cortex tension
    are commonly thought to drive cell sorting by regulating tissue surface tension
    (TST). However, the role that differential TST plays in cell segregation within
    the developing embryo is as yet unclear. Here, we have analyzed the role of differential
    TST for germ layer progenitor cell segregation during zebrafish gastrulation.
    Contrary to previous observations that differential TST drives germ layer progenitor
    cell segregation in vitro, we show that germ layers display indistinguishable
    TST within the gastrulating embryo, arguing against differential TST driving germ
    layer progenitor cell segregation in vivo. We further show that the osmolarity
    of the interstitial fluid (IF) is an important factor that influences germ layer
    TST in vivo, and that lower osmolarity of the IF compared with standard cell culture
    medium can explain why germ layers display differential TST in culture but not
    in vivo. Finally, we show that directed migration of mesendoderm progenitors is
    required for germ layer progenitor cell segregation and germ layer formation.
article_processing_charge: No
article_type: original
author:
- first_name: Gabriel
  full_name: Krens, Gabriel
  id: 2B819732-F248-11E8-B48F-1D18A9856A87
  last_name: Krens
  orcid: 0000-0003-4761-5996
- first_name: Jim
  full_name: Veldhuis, Jim
  last_name: Veldhuis
- first_name: Vanessa
  full_name: Barone, Vanessa
  id: 419EECCC-F248-11E8-B48F-1D18A9856A87
  last_name: Barone
  orcid: 0000-0003-2676-3367
- first_name: Daniel
  full_name: Capek, Daniel
  id: 31C42484-F248-11E8-B48F-1D18A9856A87
  last_name: Capek
  orcid: 0000-0001-5199-9940
- first_name: Jean-Léon
  full_name: Maître, Jean-Léon
  id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
  last_name: Maître
  orcid: 0000-0002-3688-1474
- first_name: Wayne
  full_name: Brodland, Wayne
  last_name: Brodland
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Krens G, Veldhuis J, Barone V, et al. Interstitial fluid osmolarity modulates
    the action of differential tissue surface tension in progenitor cell segregation
    during gastrulation. <i>Development</i>. 2017;144(10):1798-1806. doi:<a href="https://doi.org/10.1242/dev.144964">10.1242/dev.144964</a>
  apa: Krens, G., Veldhuis, J., Barone, V., Capek, D., Maître, J.-L., Brodland, W.,
    &#38; Heisenberg, C.-P. J. (2017). Interstitial fluid osmolarity modulates the
    action of differential tissue surface tension in progenitor cell segregation during
    gastrulation. <i>Development</i>. Company of Biologists. <a href="https://doi.org/10.1242/dev.144964">https://doi.org/10.1242/dev.144964</a>
  chicago: Krens, Gabriel, Jim Veldhuis, Vanessa Barone, Daniel Capek, Jean-Léon Maître,
    Wayne Brodland, and Carl-Philipp J Heisenberg. “Interstitial Fluid Osmolarity
    Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell
    Segregation during Gastrulation.” <i>Development</i>. Company of Biologists, 2017.
    <a href="https://doi.org/10.1242/dev.144964">https://doi.org/10.1242/dev.144964</a>.
  ieee: G. Krens <i>et al.</i>, “Interstitial fluid osmolarity modulates the action
    of differential tissue surface tension in progenitor cell segregation during gastrulation,”
    <i>Development</i>, vol. 144, no. 10. Company of Biologists, pp. 1798–1806, 2017.
  ista: Krens G, Veldhuis J, Barone V, Capek D, Maître J-L, Brodland W, Heisenberg
    C-PJ. 2017. Interstitial fluid osmolarity modulates the action of differential
    tissue surface tension in progenitor cell segregation during gastrulation. Development.
    144(10), 1798–1806.
  mla: Krens, Gabriel, et al. “Interstitial Fluid Osmolarity Modulates the Action
    of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.”
    <i>Development</i>, vol. 144, no. 10, Company of Biologists, 2017, pp. 1798–806,
    doi:<a href="https://doi.org/10.1242/dev.144964">10.1242/dev.144964</a>.
  short: G. Krens, J. Veldhuis, V. Barone, D. Capek, J.-L. Maître, W. Brodland, C.-P.J.
    Heisenberg, Development 144 (2017) 1798–1806.
date_created: 2018-12-11T11:47:52Z
date_published: 2017-05-15T00:00:00Z
date_updated: 2024-03-25T23:30:13Z
day: '15'
ddc:
- '570'
department:
- _id: Bio
- _id: CaHe
doi: 10.1242/dev.144964
external_id:
  pmid:
  - '28512197'
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language:
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month: '05'
oa: 1
oa_version: Published Version
page: 1798 - 1806
pmid: 1
publication: Development
publication_identifier:
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  - '09501991'
publication_status: published
publisher: Company of Biologists
publist_id: '7047'
quality_controlled: '1'
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  - id: '50'
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scopus_import: 1
status: public
title: Interstitial fluid osmolarity modulates the action of differential tissue surface
  tension in progenitor cell segregation during gastrulation
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 144
year: '2017'
...
---
_id: '654'
abstract:
- lang: eng
  text: In November 2016, developmental biologists, synthetic biologists and engineers
    gathered in Paris for a meeting called ‘Engineering the embryo’. The participants
    shared an interest in exploring how synthetic systems can reveal new principles
    of embryonic development, and how the in vitro manipulation and modeling of development
    using stem cells can be used to integrate ideas and expertise from physics, developmental
    biology and tissue engineering. As we review here, the conference pinpointed some
    of the challenges arising at the intersection of these fields, along with great
    enthusiasm for finding new approaches and collaborations.
author:
- first_name: Anna
  full_name: Kicheva, Anna
  id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
  last_name: Kicheva
  orcid: 0000-0003-4509-4998
- first_name: Nicolas
  full_name: Rivron, Nicolas
  last_name: Rivron
citation:
  ama: Kicheva A, Rivron N. Creating to understand – developmental biology meets engineering
    in Paris. <i>Development</i>. 2017;144(5):733-736. doi:<a href="https://doi.org/10.1242/dev.144915">10.1242/dev.144915</a>
  apa: Kicheva, A., &#38; Rivron, N. (2017). Creating to understand – developmental
    biology meets engineering in Paris. <i>Development</i>. Company of Biologists.
    <a href="https://doi.org/10.1242/dev.144915">https://doi.org/10.1242/dev.144915</a>
  chicago: Kicheva, Anna, and Nicolas Rivron. “Creating to Understand – Developmental
    Biology Meets Engineering in Paris.” <i>Development</i>. Company of Biologists,
    2017. <a href="https://doi.org/10.1242/dev.144915">https://doi.org/10.1242/dev.144915</a>.
  ieee: A. Kicheva and N. Rivron, “Creating to understand – developmental biology
    meets engineering in Paris,” <i>Development</i>, vol. 144, no. 5. Company of Biologists,
    pp. 733–736, 2017.
  ista: Kicheva A, Rivron N. 2017. Creating to understand – developmental biology
    meets engineering in Paris. Development. 144(5), 733–736.
  mla: Kicheva, Anna, and Nicolas Rivron. “Creating to Understand – Developmental
    Biology Meets Engineering in Paris.” <i>Development</i>, vol. 144, no. 5, Company
    of Biologists, 2017, pp. 733–36, doi:<a href="https://doi.org/10.1242/dev.144915">10.1242/dev.144915</a>.
  short: A. Kicheva, N. Rivron, Development 144 (2017) 733–736.
date_created: 2018-12-11T11:47:44Z
date_published: 2017-03-01T00:00:00Z
date_updated: 2021-01-12T08:07:54Z
day: '01'
ddc:
- '571'
department:
- _id: AnKi
doi: 10.1242/dev.144915
ec_funded: 1
file:
- access_level: open_access
  checksum: eef22a0f42a55b232cb2d1188a2322cb
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:15:20Z
  date_updated: 2020-07-14T12:47:33Z
  file_id: '5139'
  file_name: IST-2018-987-v1+1_2017_KichevaRivron__Creating_to.pdf
  file_size: 228206
  relation: main_file
file_date_updated: 2020-07-14T12:47:33Z
has_accepted_license: '1'
intvolume: '       144'
issue: '5'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Submitted Version
page: 733 - 736
project:
- _id: B6FC0238-B512-11E9-945C-1524E6697425
  call_identifier: H2020
  grant_number: '680037'
  name: Coordination of Patterning And Growth In the Spinal Cord
publication: Development
publication_identifier:
  issn:
  - '09501991'
publication_status: published
publisher: Company of Biologists
publist_id: '7089'
pubrep_id: '987'
quality_controlled: '1'
scopus_import: 1
status: public
title: Creating to understand – developmental biology meets engineering in Paris
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 144
year: '2017'
...