---
_id: '2615'
abstract:
- lang: eng
  text: Taste-mGluR4, cloned from taste tissues, is a truncated variant of brain-expressed
    mGluR4a (brain-mGluR4), and is known to be a candidate for the receptor involved
    in the umami taste sense. Although the expression patterns of taste- and brain-mGluR4
    mRNAs have been demonstrated, no mention has so far been made of the expression
    of these two mGluR4 proteins in taste tissues. The present study examined the
    expression of taste-mGluR4 and brain-mGluR4 proteins in rat taste tissues by using
    a specific antibody for mGluR4a which shared a C-terminus of both taste- and brain-mGluR4,
    for immunoblot analysis and immunohistochemistry. Immunoblot analysis showed that
    both brain-mGluR4 and taste-mGluR4 were expressed in the taste tissues. Taste-mGluR4
    was not detected in the cerebellum. The immunoreactive band for brain-mGluR4 protein
    was much stronger than that for taste-mGluR4 protein. In the cryosections of fungiform,
    foliate and circumvallate papillae, the antibody against taste-mGluR4 exhibited
    intense labeling of the taste pores and taste hairs in all the taste buds of gustatory
    papillae examined; the immunoreaction to the antibody against brain-mGluR4 was
    more intense at the same sites of the taste buds. The portions of the taste bud
    cells below the taste pore and surrounding keratinocytes did not show any immunoreactivities.
    The results of the present study strongly suggest that, in addition to taste-mGluR4,
    brain-mGluR4 may function even more importantly than the former as a receptor
    for glutamate, i.e. the umami taste sensation.
article_processing_charge: No
article_type: original
author:
- first_name: Takashi
  full_name: Toyono, Takashi
  last_name: Toyono
- first_name: Yuji
  full_name: Seta, Yuji
  last_name: Seta
- first_name: Shinji
  full_name: Sataoka, Shinji
  last_name: Sataoka
- first_name: Harumi
  full_name: Harada, Harumi
  id: 2E55CDF2-F248-11E8-B48F-1D18A9856A87
  last_name: Harada
  orcid: 0000-0001-7429-7896
- first_name: Takahiko
  full_name: Morotomi, Takahiko
  last_name: Morotomi
- first_name: Shintaro
  full_name: Kawano, Shintaro
  last_name: Kawano
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Kuniaki
  full_name: Toyoshima, Kuniaki
  last_name: Toyoshima
citation:
  ama: Toyono T, Seta Y, Sataoka S, et al. Expression of the metabotropic glutamate
    receptor, mGluR4a, in the taste hairs of taste buds in rat gustatory papillae.
    <i>Archives of Histology and Cytology</i>. 2002;65(1):91-96. doi:<a href="https://doi.org/10.1679/aohc.65.91">10.1679/aohc.65.91</a>
  apa: Toyono, T., Seta, Y., Sataoka, S., Harada, H., Morotomi, T., Kawano, S., …
    Toyoshima, K. (2002). Expression of the metabotropic glutamate receptor, mGluR4a,
    in the taste hairs of taste buds in rat gustatory papillae. <i>Archives of Histology
    and Cytology</i>. Japan Society of Histological Documentation. <a href="https://doi.org/10.1679/aohc.65.91">https://doi.org/10.1679/aohc.65.91</a>
  chicago: Toyono, Takashi, Yuji Seta, Shinji Sataoka, Harumi Harada, Takahiko Morotomi,
    Shintaro Kawano, Ryuichi Shigemoto, and Kuniaki Toyoshima. “Expression of the
    Metabotropic Glutamate Receptor, MGluR4a, in the Taste Hairs of Taste Buds in
    Rat Gustatory Papillae.” <i>Archives of Histology and Cytology</i>. Japan Society
    of Histological Documentation, 2002. <a href="https://doi.org/10.1679/aohc.65.91">https://doi.org/10.1679/aohc.65.91</a>.
  ieee: T. Toyono <i>et al.</i>, “Expression of the metabotropic glutamate receptor,
    mGluR4a, in the taste hairs of taste buds in rat gustatory papillae,” <i>Archives
    of Histology and Cytology</i>, vol. 65, no. 1. Japan Society of Histological Documentation,
    pp. 91–96, 2002.
  ista: Toyono T, Seta Y, Sataoka S, Harada H, Morotomi T, Kawano S, Shigemoto R,
    Toyoshima K. 2002. Expression of the metabotropic glutamate receptor, mGluR4a,
    in the taste hairs of taste buds in rat gustatory papillae. Archives of Histology
    and Cytology. 65(1), 91–96.
  mla: Toyono, Takashi, et al. “Expression of the Metabotropic Glutamate Receptor,
    MGluR4a, in the Taste Hairs of Taste Buds in Rat Gustatory Papillae.” <i>Archives
    of Histology and Cytology</i>, vol. 65, no. 1, Japan Society of Histological Documentation,
    2002, pp. 91–96, doi:<a href="https://doi.org/10.1679/aohc.65.91">10.1679/aohc.65.91</a>.
  short: T. Toyono, Y. Seta, S. Sataoka, H. Harada, T. Morotomi, S. Kawano, R. Shigemoto,
    K. Toyoshima, Archives of Histology and Cytology 65 (2002) 91–96.
date_created: 2018-12-11T11:58:41Z
date_published: 2002-01-01T00:00:00Z
date_updated: 2023-07-25T10:00:15Z
day: '01'
doi: 10.1679/aohc.65.91
extern: '1'
external_id:
  pmid:
  - '12002614'
intvolume: '        65'
issue: '1'
language:
- iso: eng
month: '01'
oa_version: None
page: 91 - 96
pmid: 1
publication: Archives of Histology and Cytology
publication_identifier:
  issn:
  - 0914-9465
publication_status: published
publisher: Japan Society of Histological Documentation
publist_id: '4283'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Expression of the metabotropic glutamate receptor, mGluR4a, in the taste hairs
  of taste buds in rat gustatory papillae
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 65
year: '2002'
...