---
_id: '2580'
abstract:
- lang: eng
text: Two group I metabotropic glutamate receptor subtypes, mGluR1 and mGluR5, have
been reported to occur in highest concentration in an annulus surrounding the
edge of the postsynaptic membrane specialisation. In order to determine whether
such a distribution is uniform amongst postsynaptic mGluRs, their distribution
was compared quantitatively by a pre-embedding silver-intensified immunogold technique
at electron microscopic level in hippocampal pyramidal cells (mGluR5), cerebellar
Purkinje cells (mGluR1α) and Golgi cells (mGluR2). The results show that mGluR1α,
mGluR5 and mGluR2 each have a distinct distribution in relation to the glutamatergic
synaptic junctions. On dendritic spines, mGluRlα and mGluR5 showed the highest
receptor density in a perisynaptic annulus (defined as within 60 nm of the edge
of the synapse) followed by a decreasing extrasynaptic (60-900 nm) receptor level,
but the gradient of decrease and the proportion of the perisynaptic pool (mGluR1α,
~ 50%; vs mGluR5, ~ 25%) were different for the two receptors. The distributions
of mGluRlα and mGluR5 also differed significantly from simulated random distributions.
In contrast, mGluR2 was not closely associated with glutamatergic synapses in
the dendritic plasma membrane of cerebellar Golgi cells and its distribution relative
to synapses is not different from simulated random distribution in the membrane.
The somatic membrane, the axon and the synaptic boutons of the GABAergic Golgi
cells also contained immunoreactive mGluR2 that is not associated with synaptic
specialisations. In the hippocampal CA1 area the distribution of immunoparticles
for mGluR5 on individual spines was established using serial sections. The results
indicate that dendritic spines of pyramidal cells are heterogeneous with respect
to the ratio of perisynaptic to extrasynaptic mGluR5 pools and about half of the
immunopositive spines lack the perisynaptic pool. The quantitative comparison
of receptor distributions demonstrates that mGluRlα and mGluR5, but not mGluR2,
are highly compartmentalised in different plasma membrane domains. The unique
distribution of each mGluR subtype may reflect requirements for different transduction
and effector mechanisms between cell types and different domains of the same cell,
and suggests that the precise placement of receptors is a crucial factor contributing
to neuronal communication.
acknowledgement: The authors are grateful to Dr Tibor Szilagyi and Mr Laszlo Marton
for advice, helpful discussions, providing the simulations of receptor distribution
and the cylindrical approximation of dendritic surface (L.M.). The brain of a mGluR2
deficient mouse was kindly provided by Drs M. Yokoi and S. Nakanishi for testing
of the specificity of one of the antibodies. The authors also thank Dr Jeff McIlhinney
for critical comments and Dr Zoltan Nusser for help with the statistics, for helpful
discussion during the project and for his comments on an earlier version of the
manuscript. The technical assistance of Ms Zahida Ahmad and the photographic assistance
of Mr Frank Kennedy, Mr Paul Jays and Mr Akira Uesugi are acknowledged. This work
was partly supported by grants from the Japan Society for the Promotion of Science,
the British Council and the Royal Society.
article_processing_charge: No
article_type: original
author:
- first_name: Rafael
full_name: Luján, Rafael
last_name: Luján
- first_name: John
full_name: Roberts, John
last_name: Roberts
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Péter
full_name: Somogyi, Péter
last_name: Somogyi
citation:
ama: Luján R, Roberts J, Shigemoto R, Ohishi H, Somogyi P. Differential plasma membrane
distribution of metabotropic glutamate receptors mGluR1α, mGluR2 and mGluR5, relative
to neurotransmitter release sites. Journal of Chemical Neuroanatomy. 1997;13(4):219-241.
doi:10.1016/S0891-0618(97)00051-3
apa: Luján, R., Roberts, J., Shigemoto, R., Ohishi, H., & Somogyi, P. (1997).
Differential plasma membrane distribution of metabotropic glutamate receptors
mGluR1α, mGluR2 and mGluR5, relative to neurotransmitter release sites. Journal
of Chemical Neuroanatomy. Elsevier. https://doi.org/10.1016/S0891-0618(97)00051-3
chicago: Luján, Rafael, John Roberts, Ryuichi Shigemoto, Hitoshi Ohishi, and Péter
Somogyi. “Differential Plasma Membrane Distribution of Metabotropic Glutamate
Receptors MGluR1α, MGluR2 and MGluR5, Relative to Neurotransmitter Release Sites.”
Journal of Chemical Neuroanatomy. Elsevier, 1997. https://doi.org/10.1016/S0891-0618(97)00051-3.
ieee: R. Luján, J. Roberts, R. Shigemoto, H. Ohishi, and P. Somogyi, “Differential
plasma membrane distribution of metabotropic glutamate receptors mGluR1α, mGluR2
and mGluR5, relative to neurotransmitter release sites,” Journal of Chemical
Neuroanatomy, vol. 13, no. 4. Elsevier, pp. 219–241, 1997.
ista: Luján R, Roberts J, Shigemoto R, Ohishi H, Somogyi P. 1997. Differential plasma
membrane distribution of metabotropic glutamate receptors mGluR1α, mGluR2 and
mGluR5, relative to neurotransmitter release sites. Journal of Chemical Neuroanatomy.
13(4), 219–241.
mla: Luján, Rafael, et al. “Differential Plasma Membrane Distribution of Metabotropic
Glutamate Receptors MGluR1α, MGluR2 and MGluR5, Relative to Neurotransmitter Release
Sites.” Journal of Chemical Neuroanatomy, vol. 13, no. 4, Elsevier, 1997,
pp. 219–41, doi:10.1016/S0891-0618(97)00051-3.
short: R. Luján, J. Roberts, R. Shigemoto, H. Ohishi, P. Somogyi, Journal of Chemical
Neuroanatomy 13 (1997) 219–241.
date_created: 2018-12-11T11:58:30Z
date_published: 1997-10-04T00:00:00Z
date_updated: 2022-08-22T12:12:03Z
day: '04'
doi: 10.1016/S0891-0618(97)00051-3
extern: '1'
external_id:
pmid:
- '9412905'
intvolume: ' 13'
issue: '4'
language:
- iso: eng
month: '10'
oa_version: None
page: 219 - 241
pmid: 1
publication: Journal of Chemical Neuroanatomy
publication_identifier:
issn:
- 0891-0618
publication_status: published
publisher: Elsevier
publist_id: '4318'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Differential plasma membrane distribution of metabotropic glutamate receptors
mGluR1α, mGluR2 and mGluR5, relative to neurotransmitter release sites
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 13
year: '1997'
...