---
_id: '8246'
abstract:
- lang: eng
  text: The Staphylococcus aureus cell wall stress stimulon (CWSS) is activated by
    cell envelope-targeting antibiotics or depletion of essential cell wall biosynthesis
    enzymes. The functionally uncharacterized S. aureus LytR-CpsA-Psr (LCP) proteins,
    MsrR, SA0908 and SA2103, all belong to the CWSS. Although not essential, deletion
    of all three LCP proteins severely impairs cell division. We show here that VraSR-dependent
    CWSS expression was up to 250-fold higher in single, double and triple LCP mutants
    than in wild type S. aureus in the absence of external stress. The LCP triple
    mutant was virtually depleted of wall teichoic acids (WTA), which could be restored
    to different degrees by any of the single LCP proteins. Subinhibitory concentrations
    of tunicamycin, which inhibits the first WTA synthesis enzyme TarO (TagO), could
    partially complement the severe growth defect of the LCP triple mutant. Both of
    the latter findings support a role for S. aureus LCP proteins in late WTA synthesis,
    as in Bacillus subtilis where LCP proteins were recently proposed to transfer
    WTA from lipid carriers to the cell wall peptidoglycan. Intrinsic activation of
    the CWSS upon LCP deletion and the fact that LCP proteins were essential for WTA-loading
    of the cell wall, highlight their important role(s) in S. aureus cell envelope
    biogenesis.
article_processing_charge: No
article_type: original
author:
- first_name: Vanina
  full_name: Dengler, Vanina
  last_name: Dengler
- first_name: Patricia Stutzmann
  full_name: Meier, Patricia Stutzmann
  last_name: Meier
- first_name: Ronald
  full_name: Heusser, Ronald
  last_name: Heusser
- first_name: Peter
  full_name: Kupferschmied, Peter
  last_name: Kupferschmied
- first_name: Judit
  full_name: Fazekas, Judit
  id: 36432834-F248-11E8-B48F-1D18A9856A87
  last_name: Fazekas
  orcid: 0000-0002-8777-3502
- first_name: Sarah
  full_name: Friebe, Sarah
  last_name: Friebe
- first_name: Sibylle Burger
  full_name: Staufer, Sibylle Burger
  last_name: Staufer
- first_name: Paul A.
  full_name: Majcherczyk, Paul A.
  last_name: Majcherczyk
- first_name: Philippe
  full_name: Moreillon, Philippe
  last_name: Moreillon
- first_name: Brigitte
  full_name: Berger-Bächi, Brigitte
  last_name: Berger-Bächi
- first_name: Nadine
  full_name: McCallum, Nadine
  last_name: McCallum
citation:
  ama: Dengler V, Meier PS, Heusser R, et al. Deletion of hypothetical wall teichoic
    acid ligases in Staphylococcus aureus activates the cell wall stress response.
    <i>FEMS Microbiology Letters</i>. 2012;333(2):109-120. doi:<a href="https://doi.org/10.1111/j.1574-6968.2012.02603.x">10.1111/j.1574-6968.2012.02603.x</a>
  apa: Dengler, V., Meier, P. S., Heusser, R., Kupferschmied, P., Singer, J., Friebe,
    S., … McCallum, N. (2012). Deletion of hypothetical wall teichoic acid ligases
    in Staphylococcus aureus activates the cell wall stress response. <i>FEMS Microbiology
    Letters</i>. Oxford University Press. <a href="https://doi.org/10.1111/j.1574-6968.2012.02603.x">https://doi.org/10.1111/j.1574-6968.2012.02603.x</a>
  chicago: Dengler, Vanina, Patricia Stutzmann Meier, Ronald Heusser, Peter Kupferschmied,
    Judit Singer, Sarah Friebe, Sibylle Burger Staufer, et al. “Deletion of Hypothetical
    Wall Teichoic Acid Ligases in Staphylococcus Aureus Activates the Cell Wall Stress
    Response.” <i>FEMS Microbiology Letters</i>. Oxford University Press, 2012. <a
    href="https://doi.org/10.1111/j.1574-6968.2012.02603.x">https://doi.org/10.1111/j.1574-6968.2012.02603.x</a>.
  ieee: V. Dengler <i>et al.</i>, “Deletion of hypothetical wall teichoic acid ligases
    in Staphylococcus aureus activates the cell wall stress response,” <i>FEMS Microbiology
    Letters</i>, vol. 333, no. 2. Oxford University Press, pp. 109–120, 2012.
  ista: Dengler V, Meier PS, Heusser R, Kupferschmied P, Singer J, Friebe S, Staufer
    SB, Majcherczyk PA, Moreillon P, Berger-Bächi B, McCallum N. 2012. Deletion of
    hypothetical wall teichoic acid ligases in Staphylococcus aureus activates the
    cell wall stress response. FEMS Microbiology Letters. 333(2), 109–120.
  mla: Dengler, Vanina, et al. “Deletion of Hypothetical Wall Teichoic Acid Ligases
    in Staphylococcus Aureus Activates the Cell Wall Stress Response.” <i>FEMS Microbiology
    Letters</i>, vol. 333, no. 2, Oxford University Press, 2012, pp. 109–20, doi:<a
    href="https://doi.org/10.1111/j.1574-6968.2012.02603.x">10.1111/j.1574-6968.2012.02603.x</a>.
  short: V. Dengler, P.S. Meier, R. Heusser, P. Kupferschmied, J. Singer, S. Friebe,
    S.B. Staufer, P.A. Majcherczyk, P. Moreillon, B. Berger-Bächi, N. McCallum, FEMS
    Microbiology Letters 333 (2012) 109–120.
date_created: 2020-08-10T11:54:47Z
date_published: 2012-08-01T00:00:00Z
date_updated: 2021-01-12T08:17:43Z
day: '01'
doi: 10.1111/j.1574-6968.2012.02603.x
extern: '1'
external_id:
  pmid:
  - '22640011'
intvolume: '       333'
issue: '2'
language:
- iso: eng
month: '08'
oa_version: None
page: 109-120
pmid: 1
publication: FEMS Microbiology Letters
publication_identifier:
  issn:
  - 0378-1097
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
status: public
title: Deletion of hypothetical wall teichoic acid ligases in Staphylococcus aureus
  activates the cell wall stress response
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 333
year: '2012'
...