---
_id: '714'
abstract:
- lang: eng
  text: Background HIV-1 infection and drug abuse are frequently co-morbid and their
    association greatly increases the severity of HIV-1-induced neuropathology. While
    nucleus accumbens (NAcc) function is severely perturbed by drugs of abuse, little
    is known about how HIV-1 infection affects NAcc. Methods We used calcium and voltage
    imaging to investigate the effect of HIV-1 trans-activator of transcription (Tat)
    on rat NAcc. Based on previous neuronal studies, we hypothesized that Tat modulates
    intracellular Ca2+ homeostasis of NAcc neurons. Results We provide evidence that
    Tat triggers a Ca2+ signaling cascade in NAcc medium spiny neurons (MSN) expressing
    D1-like dopamine receptors leading to neuronal depolarization. Firstly, Tat induced
    inositol 1,4,5-trisphsophate (IP3) receptor-mediated Ca2+ release from endoplasmic
    reticulum, followed by Ca2+ and Na+ influx via transient receptor potential canonical
    channels. The influx of cations depolarizes the membrane promoting additional
    Ca2+ entry through voltage-gated P/Q-type Ca2+ channels and opening of tetrodotoxin-sensitive
    Na+ channels. By activating this mechanism, Tat elicits a feed-forward depolarization
    increasing the excitability of D1-phosphatidylinositol-linked NAcc MSN. We previously
    found that cocaine targets NAcc neurons directly (independent of the inhibition
    of dopamine transporter) only when IP3-generating mechanisms are concomitantly
    initiated. When tested here, cocaine produced a dose-dependent potentiation of
    the effect of Tat on cytosolic Ca2+. Conclusion We describe for the first time
    a HIV-1 Tat-triggered Ca2+ signaling in MSN of NAcc involving TRPC and depolarization
    and a potentiation of the effect of Tat by cocaine, which may be relevant for
    the reward axis in cocaine-abusing HIV-1-positive patients.
acknowledgement: This work was supported by the National Institutes of Health grants
  DA035926 (to MEA), and P30DA013429 (to EMU).
article_processing_charge: No
article_type: original
author:
- first_name: Gabriela
  full_name: Brailoiu, Gabriela
  last_name: Brailoiu
- first_name: Elena
  full_name: Deliu, Elena
  id: 37A40D7E-F248-11E8-B48F-1D18A9856A87
  last_name: Deliu
  orcid: 0000-0002-7370-5293
- first_name: Jeffrey
  full_name: Barr, Jeffrey
  last_name: Barr
- first_name: Linda
  full_name: Console Bram, Linda
  last_name: Console Bram
- first_name: Alexandra
  full_name: Ciuciu, Alexandra
  last_name: Ciuciu
- first_name: Mary
  full_name: Abood, Mary
  last_name: Abood
- first_name: Ellen
  full_name: Unterwald, Ellen
  last_name: Unterwald
- first_name: Eugen
  full_name: Brǎiloiu, Eugen
  last_name: Brǎiloiu
citation:
  ama: Brailoiu G, Deliu E, Barr J, et al. HIV Tat excites D1 receptor-like expressing
    neurons from rat nucleus accumbens. <i>Drug and Alcohol Dependence</i>. 2017;178:7-14.
    doi:<a href="https://doi.org/10.1016/j.drugalcdep.2017.04.015">10.1016/j.drugalcdep.2017.04.015</a>
  apa: Brailoiu, G., Deliu, E., Barr, J., Console Bram, L., Ciuciu, A., Abood, M.,
    … Brǎiloiu, E. (2017). HIV Tat excites D1 receptor-like expressing neurons from
    rat nucleus accumbens. <i>Drug and Alcohol Dependence</i>. Elsevier. <a href="https://doi.org/10.1016/j.drugalcdep.2017.04.015">https://doi.org/10.1016/j.drugalcdep.2017.04.015</a>
  chicago: Brailoiu, Gabriela, Elena Deliu, Jeffrey Barr, Linda Console Bram, Alexandra
    Ciuciu, Mary Abood, Ellen Unterwald, and Eugen Brǎiloiu. “HIV Tat Excites D1 Receptor-like
    Expressing Neurons from Rat Nucleus Accumbens.” <i>Drug and Alcohol Dependence</i>.
    Elsevier, 2017. <a href="https://doi.org/10.1016/j.drugalcdep.2017.04.015">https://doi.org/10.1016/j.drugalcdep.2017.04.015</a>.
  ieee: G. Brailoiu <i>et al.</i>, “HIV Tat excites D1 receptor-like expressing neurons
    from rat nucleus accumbens,” <i>Drug and Alcohol Dependence</i>, vol. 178. Elsevier,
    pp. 7–14, 2017.
  ista: Brailoiu G, Deliu E, Barr J, Console Bram L, Ciuciu A, Abood M, Unterwald
    E, Brǎiloiu E. 2017. HIV Tat excites D1 receptor-like expressing neurons from
    rat nucleus accumbens. Drug and Alcohol Dependence. 178, 7–14.
  mla: Brailoiu, Gabriela, et al. “HIV Tat Excites D1 Receptor-like Expressing Neurons
    from Rat Nucleus Accumbens.” <i>Drug and Alcohol Dependence</i>, vol. 178, Elsevier,
    2017, pp. 7–14, doi:<a href="https://doi.org/10.1016/j.drugalcdep.2017.04.015">10.1016/j.drugalcdep.2017.04.015</a>.
  short: G. Brailoiu, E. Deliu, J. Barr, L. Console Bram, A. Ciuciu, M. Abood, E.
    Unterwald, E. Brǎiloiu, Drug and Alcohol Dependence 178 (2017) 7–14.
date_created: 2018-12-11T11:48:05Z
date_published: 2017-09-01T00:00:00Z
date_updated: 2021-01-12T08:12:00Z
day: '01'
department:
- _id: GaNo
doi: 10.1016/j.drugalcdep.2017.04.015
external_id:
  pmid:
  - '28623807'
intvolume: '       178'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797705
month: '09'
oa: 1
oa_version: Submitted Version
page: 7 - 14
pmid: 1
publication: Drug and Alcohol Dependence
publication_identifier:
  issn:
  - '03768716'
publication_status: published
publisher: Elsevier
publist_id: '6967'
quality_controlled: '1'
scopus_import: 1
status: public
title: HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 178
year: '2017'
...