---
_id: '747'
abstract:
- lang: eng
  text: Bradykinin (BK), a component of the kallikrein-kininogen-kinin system exerts
    multiple effects via B1 and B2 receptor activation. In the cardiovascular system,
    bradykinin has cardioprotective and vasodilator properties. We investigated the
    effect of BK on cardiac-projecting neurons of nucleus ambiguus, a key site for
    the parasympathetic cardiac regulation. BK produced a dose-dependent increase
    in cytosolic Ca2+ concentration. Pretreatment with HOE140, a B2 receptor antagonist,
    but not with R715, a B1 receptor antagonist, abolished the response to BK. A selective
    B2 receptor agonist, but not a B1 receptor agonist, elicited an increase in cytosolic
    Ca2+ similarly to BK. Inhibition of N-type voltage-gated Ca2+ channels with ω-conotoxin
    GVIA had no effect on the Ca2+ signal produced by BK, while pretreatment with
    ω-conotoxin MVIIC, a blocker of P/Q-type of Ca2+ channels, significantly diminished
    the effect of BK. Pretreatment with xestospongin C and 2-aminoethoxydiphenyl borate,
    antagonists of inositol 1,4,5-trisphosphate receptors, abolished the response
    to BK. Inhibition of ryanodine receptors reduced the BK-induced Ca2+ increase,
    while disruption of lysosomal Ca2+ stores with bafilomycin A1 did not affect the
    response. BK produced a dose-dependent depolarization of nucleus ambiguus neurons,
    which was prevented by the B2 receptor antagonist. In vivo studies indicate that
    microinjection of BK into nucleus ambiguus elicited bradycardia in conscious rats
    via B2 receptors. In summary, in cardiac vagal neurons of nucleus ambiguus, BK
    activates B2 receptors promoting Ca2+ influx and Ca2+ release from endoplasmic
    reticulum, and membrane depolarization; these effects are translated in vivo by
    bradycardia.
article_processing_charge: No
article_type: original
author:
- first_name: Eugen
  full_name: Brǎiloiu, Eugen
  last_name: Brǎiloiu
- first_name: Matthew
  full_name: Mcguire, Matthew
  last_name: Mcguire
- first_name: Shadaria
  full_name: Shuler, Shadaria
  last_name: Shuler
- first_name: Elena
  full_name: Deliu, Elena
  id: 37A40D7E-F248-11E8-B48F-1D18A9856A87
  last_name: Deliu
  orcid: 0000-0002-7370-5293
- first_name: Jeffrey
  full_name: Barr, Jeffrey
  last_name: Barr
- first_name: Mary
  full_name: Abood, Mary
  last_name: Abood
- first_name: Gabriela
  full_name: Brailoiu, Gabriela
  last_name: Brailoiu
citation:
  ama: Brǎiloiu E, Mcguire M, Shuler S, et al. Modulation of cardiac vagal tone by
    bradykinin acting on nucleus ambiguus. <i>Neuroscience</i>. 2017;365:23-32. doi:<a
    href="https://doi.org/10.1016/j.neuroscience.2017.09.034">10.1016/j.neuroscience.2017.09.034</a>
  apa: Brǎiloiu, E., Mcguire, M., Shuler, S., Deliu, E., Barr, J., Abood, M., &#38;
    Brailoiu, G. (2017). Modulation of cardiac vagal tone by bradykinin acting on
    nucleus ambiguus. <i>Neuroscience</i>. Elsevier. <a href="https://doi.org/10.1016/j.neuroscience.2017.09.034">https://doi.org/10.1016/j.neuroscience.2017.09.034</a>
  chicago: Brǎiloiu, Eugen, Matthew Mcguire, Shadaria Shuler, Elena Deliu, Jeffrey
    Barr, Mary Abood, and Gabriela Brailoiu. “Modulation of Cardiac Vagal Tone by
    Bradykinin Acting on Nucleus Ambiguus.” <i>Neuroscience</i>. Elsevier, 2017. <a
    href="https://doi.org/10.1016/j.neuroscience.2017.09.034">https://doi.org/10.1016/j.neuroscience.2017.09.034</a>.
  ieee: E. Brǎiloiu <i>et al.</i>, “Modulation of cardiac vagal tone by bradykinin
    acting on nucleus ambiguus,” <i>Neuroscience</i>, vol. 365. Elsevier, pp. 23–32,
    2017.
  ista: Brǎiloiu E, Mcguire M, Shuler S, Deliu E, Barr J, Abood M, Brailoiu G. 2017.
    Modulation of cardiac vagal tone by bradykinin acting on nucleus ambiguus. Neuroscience.
    365, 23–32.
  mla: Brǎiloiu, Eugen, et al. “Modulation of Cardiac Vagal Tone by Bradykinin Acting
    on Nucleus Ambiguus.” <i>Neuroscience</i>, vol. 365, Elsevier, 2017, pp. 23–32,
    doi:<a href="https://doi.org/10.1016/j.neuroscience.2017.09.034">10.1016/j.neuroscience.2017.09.034</a>.
  short: E. Brǎiloiu, M. Mcguire, S. Shuler, E. Deliu, J. Barr, M. Abood, G. Brailoiu,
    Neuroscience 365 (2017) 23–32.
date_created: 2018-12-11T11:48:17Z
date_published: 2017-12-04T00:00:00Z
date_updated: 2023-09-27T12:26:59Z
day: '04'
department:
- _id: GaNo
doi: 10.1016/j.neuroscience.2017.09.034
external_id:
  isi:
  - '000415966200003'
  pmid:
  - '28951324'
intvolume: '       365'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798458
month: '12'
oa: 1
oa_version: Submitted Version
page: 23 - 32
pmid: 1
publication: Neuroscience
publication_identifier:
  issn:
  - '03064522'
publication_status: published
publisher: Elsevier
publist_id: '6911'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Modulation of cardiac vagal tone by bradykinin acting on nucleus ambiguus
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 365
year: '2017'
...