---
_id: '8914'
abstract:
- lang: eng
text: Amyotrophic lateral sclerosis (ALS) leads to a loss of specific motor neuron
populations in the spinal cord and cortex. Emerging evidence suggests that interneurons
may also be affected, but a detailed characterization of interneuron loss and
its potential impacts on motor neuron loss and disease progression is lacking.
To examine this issue, the fate of V1 inhibitory neurons during ALS was assessed
in the ventral spinal cord using the SODG93A mouse model. The V1 population makes
up ∼30% of all ventral inhibitory neurons, ∼50% of direct inhibitory synaptic
contacts onto motor neuron cell bodies, and is thought to play a key role in modulating
motor output, in part through recurrent and reciprocal inhibitory circuits. We
find that approximately half of V1 inhibitory neurons are lost in SODG93A mice
at late disease stages, but that this loss is delayed relative to the loss of
motor neurons and V2a excitatory neurons. We further identify V1 subpopulations
based on transcription factor expression that are differentially susceptible to
degeneration in SODG93A mice. At an early disease stage, we show that V1 synaptic
contacts with motor neuron cell bodies increase, suggesting an upregulation of
inhibition before V1 neurons are lost in substantial numbers. These data support
a model in which progressive changes in V1 synaptic contacts early in disease,
and in select V1 subpopulations at later stages, represent a compensatory upregulation
and then deleterious breakdown of specific interneuron circuits within the spinal
cord.
acknowledgement: This work was made possible by the generous support of Project ALS.
Imaging and related analyses were facilitated by The Waitt Advanced Biophotonics
Center Core at the Salk Institute, supported by grants from NIH-NCI CCSG (P30 014195)
and NINDS Neuroscience Center (NS072031). The authors would like to additionally
thank Drs. Jane Dodd, Robert Brownstone, and Laskaro Zagoraiou for helpful comments
on the manuscript. This manuscript is dedicated to Tom Jessell, an inspirational
scientist, friend and mentor.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alina
full_name: Salamatina, Alina
last_name: Salamatina
- first_name: Jerry H
full_name: Yang, Jerry H
last_name: Yang
- first_name: Susan
full_name: Brenner-Morton, Susan
last_name: Brenner-Morton
- first_name: 'Jay B '
full_name: 'Bikoff, Jay B '
last_name: Bikoff
- first_name: Linjing
full_name: Fang, Linjing
last_name: Fang
- first_name: Christopher R
full_name: Kintner, Christopher R
last_name: Kintner
- first_name: Thomas M
full_name: Jessell, Thomas M
last_name: Jessell
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Salamatina A, Yang JH, Brenner-Morton S, et al. Differential loss of spinal
interneurons in a mouse model of ALS. Neuroscience. 2020;450:81-95. doi:10.1016/j.neuroscience.2020.08.011
apa: Salamatina, A., Yang, J. H., Brenner-Morton, S., Bikoff, J. B., Fang, L., Kintner,
C. R., … Sweeney, L. B. (2020). Differential loss of spinal interneurons in a
mouse model of ALS. Neuroscience. Elsevier. https://doi.org/10.1016/j.neuroscience.2020.08.011
chicago: Salamatina, Alina, Jerry H Yang, Susan Brenner-Morton, Jay B Bikoff, Linjing
Fang, Christopher R Kintner, Thomas M Jessell, and Lora B. Sweeney. “Differential
Loss of Spinal Interneurons in a Mouse Model of ALS.” Neuroscience. Elsevier,
2020. https://doi.org/10.1016/j.neuroscience.2020.08.011.
ieee: A. Salamatina et al., “Differential loss of spinal interneurons in
a mouse model of ALS,” Neuroscience, vol. 450. Elsevier, pp. 81–95, 2020.
ista: Salamatina A, Yang JH, Brenner-Morton S, Bikoff JB, Fang L, Kintner CR, Jessell
TM, Sweeney LB. 2020. Differential loss of spinal interneurons in a mouse model
of ALS. Neuroscience. 450, 81–95.
mla: Salamatina, Alina, et al. “Differential Loss of Spinal Interneurons in a Mouse
Model of ALS.” Neuroscience, vol. 450, Elsevier, 2020, pp. 81–95, doi:10.1016/j.neuroscience.2020.08.011.
short: A. Salamatina, J.H. Yang, S. Brenner-Morton, J.B. Bikoff, L. Fang, C.R. Kintner,
T.M. Jessell, L.B. Sweeney, Neuroscience 450 (2020) 81–95.
date_created: 2020-12-03T11:47:31Z
date_published: 2020-12-01T00:00:00Z
date_updated: 2024-01-31T10:15:34Z
day: '01'
ddc:
- '570'
department:
- _id: LoSw
doi: 10.1016/j.neuroscience.2020.08.011
external_id:
isi:
- '000595588700008'
pmid:
- '32858144'
file:
- access_level: open_access
checksum: da7413c819e079720669c82451b49294
content_type: application/pdf
creator: dernst
date_created: 2020-12-03T11:45:26Z
date_updated: 2020-12-03T11:45:26Z
file_id: '8915'
file_name: 2020_Neuroscience_Salamatina.pdf
file_size: 4071247
relation: main_file
success: 1
file_date_updated: 2020-12-03T11:45:26Z
has_accepted_license: '1'
intvolume: ' 450'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '12'
oa: 1
oa_version: Published Version
page: 81-95
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Differential loss of spinal interneurons in a mouse model of ALS
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 450
year: '2020'
...
---
_id: '2605'
abstract:
- lang: eng
text: 'The granular layer of the cerebellar cortex consists of densely packed neuronal
cells, classified into granule cells and large interneurons. In this study, we
provide a comparative survey of large granular layer interneurons in the adult
rat cerebellum based on both morphological and neurochemical criteria. To this
end, double immunofluorescence histochemistry was performed by combining antibodies
against the cytoplasmic antigen Rat-303, calretinin, the metabotropic glutamate
receptor mGluR2 and somatostatin. Based on Rat-303/calretinin double immunohistochemistry,
three distinct populations of large granular layer interneurons could be discerned:
cells immunopositive for Rat-303, calretinin or both. Rat-303 or calretinin single-labeled
cells represented Golgi cells and unipolar brush cells, respectively. Rat-303/calretinin
double-labeled cells located just underneath the Purkinje cell layer represented
Lugaro cells. Morphometrical analysis distinguished two populations of Rat-303-positive
Golgi cells according to their location: vermis versus hemisphere. Immunostaining
for the metabotropic glutamate receptor mGluR2 combined with Rat-303 or calretinin
revealed that the majority of Golgi cells (about 90%) appeared to be mGluR2 positive.
Lugaro cells were mGluR2 negative. In addition, a limited population of large
polymorphous interneurons in the depth of the granular layer with morphological
features resembling Golgi cells also displayed Rat-303/calretinin immunoreactivity
and were mGluR2 negative. Double immunohistochemistry for Rat-303 and somatostatin
revealed three populations of labeled cells in the depth of the granular layer.
Besides double-labeled Golgi cells, Rat-303 or somatostatin single-labeled cells
were present. Based on mGluR2/somatostatin and calretinin/somatostatin double
immunostainings, Rat-303 single-labeled cells were found to correspond to Rat-303/calretinin-positive,
mGluR2-negative Golgi-like cells, while the identity of somatostatin single-labeled
cells remained unclear. The data presented in this article elaborate previous
reports on the morphological and neurochemical differentiation of large interneurons
in the rat cerebellar granular layer. In addition, they indicate that the current
classification of these cells into Golgi cells, Lugaro cells and unipolar brush
cells does not describe the observed neurochemical heterogeneity.'
article_processing_charge: No
article_type: original
author:
- first_name: Frederik
full_name: Geurts, Frederik
last_name: Geurts
- first_name: Jean
full_name: Timmermans, Jean
last_name: Timmermans
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Erik
full_name: De Schutter, Erik
last_name: De Schutter
citation:
ama: Geurts F, Timmermans J, Shigemoto R, De Schutter E. Morphological and neurochemical
differentiation of large granular layer interneurons in the adult rat cerebellum.
Neuroscience. 2001;104(2):499-512. doi:10.1016/S0306-4522(01)00058-6
apa: Geurts, F., Timmermans, J., Shigemoto, R., & De Schutter, E. (2001). Morphological
and neurochemical differentiation of large granular layer interneurons in the
adult rat cerebellum. Neuroscience. Elsevier. https://doi.org/10.1016/S0306-4522(01)00058-6
chicago: Geurts, Frederik, Jean Timmermans, Ryuichi Shigemoto, and Erik De Schutter.
“Morphological and Neurochemical Differentiation of Large Granular Layer Interneurons
in the Adult Rat Cerebellum.” Neuroscience. Elsevier, 2001. https://doi.org/10.1016/S0306-4522(01)00058-6.
ieee: F. Geurts, J. Timmermans, R. Shigemoto, and E. De Schutter, “Morphological
and neurochemical differentiation of large granular layer interneurons in the
adult rat cerebellum,” Neuroscience, vol. 104, no. 2. Elsevier, pp. 499–512,
2001.
ista: Geurts F, Timmermans J, Shigemoto R, De Schutter E. 2001. Morphological and
neurochemical differentiation of large granular layer interneurons in the adult
rat cerebellum. Neuroscience. 104(2), 499–512.
mla: Geurts, Frederik, et al. “Morphological and Neurochemical Differentiation of
Large Granular Layer Interneurons in the Adult Rat Cerebellum.” Neuroscience,
vol. 104, no. 2, Elsevier, 2001, pp. 499–512, doi:10.1016/S0306-4522(01)00058-6.
short: F. Geurts, J. Timmermans, R. Shigemoto, E. De Schutter, Neuroscience 104
(2001) 499–512.
date_created: 2018-12-11T11:58:38Z
date_published: 2001-05-10T00:00:00Z
date_updated: 2023-05-24T12:45:30Z
day: '10'
doi: 10.1016/S0306-4522(01)00058-6
extern: '1'
external_id:
pmid:
- '11377850'
intvolume: ' 104'
issue: '2'
language:
- iso: eng
month: '05'
oa_version: None
page: 499 - 512
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '4292'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Morphological and neurochemical differentiation of large granular layer interneurons
in the adult rat cerebellum
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 104
year: '2001'
...
---
_id: '2608'
abstract:
- lang: eng
text: The regulation of neurotransmitter receptors during synapse formation has
been studied extensively at the neuromuscular junction, but little is known about
the development of excitatory neurotransmitter receptors during synaptogenesis
in central synapses. In this study we show qualitatively and quantitatively that
a receptor undergoes changes in localisation on the surface of rat Purkinje cells
during development in association with its excitatory synapses. The presence of
mGluR1α at parallel and climbing fibre synapses on developing Purkinje cells was
studied using high-resolution immunoelectron microscopy. Immunoreactivity for
mGluR1α was detected from embryonic day 18 in Purkinje cells, and showed dramatic
changes in its localisation with age. At early postnatal ages (P0 and P3), mGluR1α
was found both in somata and stem dendrites but was not usually associated with
synaptic contacts. At P7, mGluR1α became concentrated in somatic spines associated
with climbing fibres and in the growing dendritic arborisation even before innervation
by parallel fibres. During the second and third postnatal week, when spines and
parallel fibre synapses were generated, mGluR1α became progressively concentrated
in the molecular layer, particularly in the synaptic specialisations. As a result,
during the fourth postnatal week, the pattern and level of mGluR1α expression
became similar to the adult and mGluR1α appeared in high density in perisynaptic
sites. Our results indicate that mGluR1α is present in the developing Purkinje
cells prior to their innervation by climbing and parallel fibres and demonstrate
that this receptor undergoes a dynamic and specific regulation during postnatal
development in association with the establishment of synaptic inputs to Purkinje
cell.
acknowledgement: öWe thank Drs. Paul Bolam, Ole Paulsen, Je¡ McIlhinney, Alfonso Faire¨n
and Francisco Ciruela for reviewing a previous version of this manuscript and Mrs
Alexandra Salewski for the English revision of the manuscript. We also want to thank
Dr. Peter Somogyi for offering the facilities of the MRC Anatomical Neuropharmacology
Unit to carry out part of this study. This work was supported by a Grant from the
European Community (QLG3-CT-1999-00192 to R.L.) and the Spanish Ministry of Education
(DGES PM 97-0082 to J.M.J.).
article_processing_charge: No
article_type: original
author:
- first_name: Guillermina
full_name: López Bendito, Guillermina
last_name: López Bendito
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Rafael
full_name: Luján, Rafael
last_name: Luján
- first_name: José
full_name: Juíz, José
last_name: Juíz
citation:
ama: López Bendito G, Shigemoto R, Luján R, Juíz J. Developmental changes in the
localisation of the mGluR1α subtype of metabotropic glutamate receptors in Purkinje
cells. Neuroscience. 2001;105(2):413-429. doi:10.1016/S0306-4522(01)00188-9
apa: López Bendito, G., Shigemoto, R., Luján, R., & Juíz, J. (2001). Developmental
changes in the localisation of the mGluR1α subtype of metabotropic glutamate receptors
in Purkinje cells. Neuroscience. Elsevier. https://doi.org/10.1016/S0306-4522(01)00188-9
chicago: López Bendito, Guillermina, Ryuichi Shigemoto, Rafael Luján, and José Juíz.
“Developmental Changes in the Localisation of the MGluR1α Subtype of Metabotropic
Glutamate Receptors in Purkinje Cells.” Neuroscience. Elsevier, 2001. https://doi.org/10.1016/S0306-4522(01)00188-9.
ieee: G. López Bendito, R. Shigemoto, R. Luján, and J. Juíz, “Developmental changes
in the localisation of the mGluR1α subtype of metabotropic glutamate receptors
in Purkinje cells,” Neuroscience, vol. 105, no. 2. Elsevier, pp. 413–429,
2001.
ista: López Bendito G, Shigemoto R, Luján R, Juíz J. 2001. Developmental changes
in the localisation of the mGluR1α subtype of metabotropic glutamate receptors
in Purkinje cells. Neuroscience. 105(2), 413–429.
mla: López Bendito, Guillermina, et al. “Developmental Changes in the Localisation
of the MGluR1α Subtype of Metabotropic Glutamate Receptors in Purkinje Cells.”
Neuroscience, vol. 105, no. 2, Elsevier, 2001, pp. 413–29, doi:10.1016/S0306-4522(01)00188-9.
short: G. López Bendito, R. Shigemoto, R. Luján, J. Juíz, Neuroscience 105 (2001)
413–429.
date_created: 2018-12-11T11:58:39Z
date_published: 2001-07-27T00:00:00Z
date_updated: 2023-05-24T09:31:48Z
day: '27'
doi: 10.1016/S0306-4522(01)00188-9
extern: '1'
external_id:
pmid:
- '11672608 '
intvolume: ' 105'
issue: '2'
language:
- iso: eng
month: '07'
oa_version: None
page: 413 - 429
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '4290'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Developmental changes in the localisation of the mGluR1α subtype of metabotropic
glutamate receptors in Purkinje cells
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 105
year: '2001'
...
---
_id: '2609'
abstract:
- lang: eng
text: 'The metabotropic glutamate receptors (mGluRs) have distinct distribution
patterns in the CNS but subtypes within group I or group III mGluRs share similar
ultrastructural localization relative to neurotransmitter release sites: group
I mGluRs are concentrated in an annulus surrounding the edge of the postsynaptic
density, whereas group III mGluRs are concentrated in the presynaptic active zone.
One of the group II subtypes, mGluR2, is expressed in both pre- and postsynaptic
elements, having no close association with synapses. In order to determine if
such a distribution is common to another group II subtype, mGluR3, an antibody
was raised against a carboxy-terminus of mGluR3 and used for light and electron
microscopic immunohistochemistry in the mouse CNS. The antibody reacted strongly
with mGluR3, but it also reacted, though only weakly, with mGluR2. Therefore,
to examine mGluR3-selective distribution, we used mGluR2-deficient mice as well
as wild-type mice. Strong immunoreactivity for mGluR3 was found in the cerebral
cortex, striatum, dentate gyrus of the hippocampus, olfactory tubercle, lateral
septal nucleus, lateral and basolateral amygdaloid nuclei, and nucleus of the
lateral olfactory tract. Pre-embedding immunoperoxidase and immunogold methods
revealed mGluR3 labeling in both presynaptic and postsynaptic elements, and also
in glial profiles. Double labeling revealed that the vast majority of mGluR3 in
presynaptic elements is not closely associated with glutamate and GABA release
sites in the striatum and thalamus, respectively. However, in the spines of the
dentate granule cells, the highest receptor density was found in perisynaptic
sites (20% of immunogold particles within 60 nm from the edge of postsynaptic
membrane specialization) followed by a decreasing receptor density away from the
synapses (to ∼5% of particles per 60 nm). Furthermore, 19% of immunogold particles
were located in asymmetrical postsynaptic specialization, indicating an association
of mGluR3 to glutamatergic synapses. The present results indicate that the localization
of mGluR3 is rather similar to that of group I mGluRs in the postsynaptic elements,
suggesting a unique functional role of mGluR3 in glutamatergic neurotransmission
in the CNS.'
acknowledgement: We are grateful to M. Yokoi and S. Nakanishi for kindly providing
us with the mGluR2-de¢cient mice and F. Ferraguti for mGluR8b cDNA. The technical
assistance of S. Doi and the photographic assistance of A. Uesugi are acknowledged.
This work has been supported by research grants from the Ministry of Education,
Sports, Culture, Science, and Technology of Japan.
article_processing_charge: No
article_type: original
author:
- first_name: Y
full_name: Tamaru, Y
last_name: Tamaru
- first_name: Sakashi
full_name: Nomura, Sakashi
last_name: Nomura
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: 'Tamaru Y, Nomura S, Mizuno N, Shigemoto R. Distribution of metabotropic glutamate
receptor mGluR3 in the mouse CNS: Differential location relative to pre- and postsynaptic
sites. Neuroscience. 2001;106(3):481-503. doi:10.1016/S0306-4522(01)00305-0'
apa: 'Tamaru, Y., Nomura, S., Mizuno, N., & Shigemoto, R. (2001). Distribution
of metabotropic glutamate receptor mGluR3 in the mouse CNS: Differential location
relative to pre- and postsynaptic sites. Neuroscience. Elsevier. https://doi.org/10.1016/S0306-4522(01)00305-0'
chicago: 'Tamaru, Y, Sakashi Nomura, Noboru Mizuno, and Ryuichi Shigemoto. “Distribution
of Metabotropic Glutamate Receptor MGluR3 in the Mouse CNS: Differential Location
Relative to Pre- and Postsynaptic Sites.” Neuroscience. Elsevier, 2001.
https://doi.org/10.1016/S0306-4522(01)00305-0.'
ieee: 'Y. Tamaru, S. Nomura, N. Mizuno, and R. Shigemoto, “Distribution of metabotropic
glutamate receptor mGluR3 in the mouse CNS: Differential location relative to
pre- and postsynaptic sites,” Neuroscience, vol. 106, no. 3. Elsevier,
pp. 481–503, 2001.'
ista: 'Tamaru Y, Nomura S, Mizuno N, Shigemoto R. 2001. Distribution of metabotropic
glutamate receptor mGluR3 in the mouse CNS: Differential location relative to
pre- and postsynaptic sites. Neuroscience. 106(3), 481–503.'
mla: 'Tamaru, Y., et al. “Distribution of Metabotropic Glutamate Receptor MGluR3
in the Mouse CNS: Differential Location Relative to Pre- and Postsynaptic Sites.”
Neuroscience, vol. 106, no. 3, Elsevier, 2001, pp. 481–503, doi:10.1016/S0306-4522(01)00305-0.'
short: Y. Tamaru, S. Nomura, N. Mizuno, R. Shigemoto, Neuroscience 106 (2001) 481–503.
date_created: 2018-12-11T11:58:39Z
date_published: 2001-09-27T00:00:00Z
date_updated: 2023-05-24T08:51:17Z
day: '27'
doi: 10.1016/S0306-4522(01)00305-0
extern: '1'
external_id:
pmid:
- '11591452'
intvolume: ' 106'
issue: '3'
language:
- iso: eng
month: '09'
oa_version: None
page: 481 - 503
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '4289'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Distribution of metabotropic glutamate receptor mGluR3 in the mouse CNS: Differential
location relative to pre- and postsynaptic sites'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 106
year: '2001'
...
---
_id: '2611'
abstract:
- lang: eng
text: Research using animal models of neuropathic pain has revealed sympathetic
sprouting onto dorsal root ganglion cells. More recently, sensory fibre sprouting
onto dorsal root ganglion cells has also been observed. Previous work in our laboratory
demonstrated persistent sympathetic fibre sprouting in the skin of the rat lower
lip following sensory denervation of this region. Therefore, we applied immunocytochemistry
to determine the effects of sympathectomies on the terminal fields of sensory
fibres. The superior cervical ganglia were removed bilaterally and the effects
on the innervation of the skin of the rat lower lip were observed 1, 2, 3, 4,
6 and 8 weeks post-surgery. Substance P and dopamine-β-hydroxylase immunoreactivities
were used to identify a subset of sensory and sympathetic fibres, respectively.
We also assessed neurokinin-1 receptor immunoreactivity. Quantitative data was
obtained with the aid of an image analysis system. In controls, the epidermis
and upper dermis were innervated by substance P-immunoreactive fibres only and
upper dermal blood vessels possessed the highest density of neurokinin-1 receptor
immunoreactivity. Blood vessels in the lower dermis were innervated by both substance
P- and dopamine-β-hydroxylase-immunoreactive fibres. Following sympathectomies,
substance P-immunoreactive fibres in the epidermis and upper dermis were more
intensely labelled only 1 and 2 weeks post-surgery when compared to sham controls.
The length of substance P-immunoreactive fibres in this region was also increased
only on the second week. Neurokinin-1 receptor immunoreactivity in the upper dermis
was slightly decreased 1 and 2 weeks post-surgery. In the lower dermis, substance
P-immunoreactive fibres associated with blood vessels were more intensely labelled
only 1 and 2 weeks post-surgery, and at all post-surgical time points studied,
blood vessels in this region were devoid of dopamine-β-hydroxylase-immunoreactive
fibres. The length of substance P-immunoreactive fibres was increased from the
first to the third week post-surgery in the lower dermis. These results indicate
that sympathectomies lead to transient changes in substance P-immunoreactive fibre
innervation and neurokinin-1 receptor expression in rat lower lip skin. The effects
are most prominent in the lower dermis probably due to a greater local concentration
of nerve growth factor in this region. The plasticity of the interactions between
sensory and sympathetic fibres may prove important in the regulation of skin microcirculation
and in the generation of painful sensations under normal conditions or following
peripheral nerve injuries.
acknowledgement: 'The work contained in this manuscript was sponsored by the Canadian
MRC, Grants # MT-12170 and MoP-38093. The authors would like to thank Sylvain Cote
for technical assistance and Sid Parkinson for editorial assistance.'
article_processing_charge: No
article_type: original
author:
- first_name: Isabella
full_name: Ruocco, Isabella
last_name: Ruocco
- first_name: Augusto
full_name: Cuello, Augusto
last_name: Cuello
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Alfredo
full_name: Ribeiro Da Silva, Alfredo
last_name: Ribeiro Da Silva
citation:
ama: Ruocco I, Cuello A, Shigemoto R, Ribeiro Da Silva A. Sympathectomies lead to
transient substance P-immunoreactive sensory fibre plasticity in the rat skin.
Neuroscience. 2001;108(1):157-166. doi:10.1016/S0306-4522(01)00158-0
apa: Ruocco, I., Cuello, A., Shigemoto, R., & Ribeiro Da Silva, A. (2001). Sympathectomies
lead to transient substance P-immunoreactive sensory fibre plasticity in the rat
skin. Neuroscience. Elsevier. https://doi.org/10.1016/S0306-4522(01)00158-0
chicago: Ruocco, Isabella, Augusto Cuello, Ryuichi Shigemoto, and Alfredo Ribeiro
Da Silva. “Sympathectomies Lead to Transient Substance P-Immunoreactive Sensory
Fibre Plasticity in the Rat Skin.” Neuroscience. Elsevier, 2001. https://doi.org/10.1016/S0306-4522(01)00158-0.
ieee: I. Ruocco, A. Cuello, R. Shigemoto, and A. Ribeiro Da Silva, “Sympathectomies
lead to transient substance P-immunoreactive sensory fibre plasticity in the rat
skin,” Neuroscience, vol. 108, no. 1. Elsevier, pp. 157–166, 2001.
ista: Ruocco I, Cuello A, Shigemoto R, Ribeiro Da Silva A. 2001. Sympathectomies
lead to transient substance P-immunoreactive sensory fibre plasticity in the rat
skin. Neuroscience. 108(1), 157–166.
mla: Ruocco, Isabella, et al. “Sympathectomies Lead to Transient Substance P-Immunoreactive
Sensory Fibre Plasticity in the Rat Skin.” Neuroscience, vol. 108, no.
1, Elsevier, 2001, pp. 157–66, doi:10.1016/S0306-4522(01)00158-0.
short: I. Ruocco, A. Cuello, R. Shigemoto, A. Ribeiro Da Silva, Neuroscience 108
(2001) 157–166.
date_created: 2018-12-11T11:58:40Z
date_published: 2001-12-05T00:00:00Z
date_updated: 2023-05-22T12:15:44Z
day: '05'
doi: 10.1016/S0306-4522(01)00158-0
extern: '1'
external_id:
pmid:
- '11738139'
intvolume: ' 108'
issue: '1'
language:
- iso: eng
month: '12'
oa_version: None
page: 157 - 166
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '4286'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Sympathectomies lead to transient substance P-immunoreactive sensory fibre
plasticity in the rat skin
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 108
year: '2001'
...
---
_id: '3515'
abstract:
- lang: eng
text: Oscillations in neuronal networks are assumed to serve various physiological
functions, from coordination of motor patterns to perceptual binding of sensory
information. Here, we describe an ultra-slow oscillation (0.025 Hz) in the hippocampus.
Extracellular and intracellular activity was recorded from the CA1 and subicular
regions in rats of the Wistar and Sprague-Dawley strains. anesthetized with urethane.
in a subgroup of Wistar rats (23%), spontaneous afterdischarges (4.7 +/- 1.6 s)
occurred regularly at 40.8 +/- 15.7 s. The afterdischarge was initiated by a fast
increase of population synchrony (100-250 Hz oscillation; “tonic” phase), followed
by large-amplitude rhythmic waves and associated action potentials at gamma and
beta frequency (15-50 Hz; “clonic” phase). The afterdischarges were bilaterally
synchronous and terminated relatively abruptly without post-ictal depression.
Single-pulse stimulation of the commissural input could trigger afterdischarges,
but only at times when they were about to occur. Commissural stimulation evoked
inhibitory postsynaptic potentials in pyramidal cells. However, when the stimulus
triggered an afterdischarge, the inhibitory postsynaptic potential was absent
and the cells remained depolarized during most of the afterdischarge. Afterdischarges
were not observed in the Sprague-Dawley rats. Long-term analysis of interneuronal
activity in intact, drug-free rats also revealed periodic excitability changes
in the hippocampal network at 0.025 Hz. These findings indicate the presence of
an ultra-slow oscillation in the hippocampal formation. The ultra-slow clock induced
afterdischarges in susceptible animals. We hypothesize that a transient failure
of GABAergic inhibition in a subset of Wistar rats is responsible for the emergence
of epileptiform patterns. (C) 1999 IBRO. Published by Elsevier Science Ltd.
acknowledgement: This work was supported by the Academy of Finland (32391) and the
NIH (NS34994, MH54671).
article_processing_charge: No
article_type: original
author:
- first_name: Markku
full_name: Penttonen, Markku
last_name: Penttonen
- first_name: Nina
full_name: Nurminen, Nina
last_name: Nurminen
- first_name: Riitta
full_name: Miettinen, Riitta
last_name: Miettinen
- first_name: Jouni
full_name: Sirviö, Jouni
last_name: Sirviö
- first_name: Darrell
full_name: Henze, Darrell
last_name: Henze
- first_name: Jozsef L
full_name: Csicsvari, Jozsef L
id: 3FA14672-F248-11E8-B48F-1D18A9856A87
last_name: Csicsvari
orcid: 0000-0002-5193-4036
- first_name: György
full_name: Buzsáki, György
last_name: Buzsáki
citation:
ama: Penttonen M, Nurminen N, Miettinen R, et al. Ultra-slow oscillation (0.025
Hz) triggers hippocampal afterdischarges in Wistar rats. Neuroscience.
1999;94(3):735-743. doi:10.1016/S0306-4522(99)00367-X
apa: Penttonen, M., Nurminen, N., Miettinen, R., Sirviö, J., Henze, D., Csicsvari,
J. L., & Buzsáki, G. (1999). Ultra-slow oscillation (0.025 Hz) triggers hippocampal
afterdischarges in Wistar rats. Neuroscience. Elsevier. https://doi.org/10.1016/S0306-4522(99)00367-X
chicago: Penttonen, Markku, Nina Nurminen, Riitta Miettinen, Jouni Sirviö, Darrell
Henze, Jozsef L Csicsvari, and György Buzsáki. “Ultra-Slow Oscillation (0.025
Hz) Triggers Hippocampal Afterdischarges in Wistar Rats.” Neuroscience.
Elsevier, 1999. https://doi.org/10.1016/S0306-4522(99)00367-X.
ieee: M. Penttonen et al., “Ultra-slow oscillation (0.025 Hz) triggers hippocampal
afterdischarges in Wistar rats,” Neuroscience, vol. 94, no. 3. Elsevier,
pp. 735–743, 1999.
ista: Penttonen M, Nurminen N, Miettinen R, Sirviö J, Henze D, Csicsvari JL, Buzsáki
G. 1999. Ultra-slow oscillation (0.025 Hz) triggers hippocampal afterdischarges
in Wistar rats. Neuroscience. 94(3), 735–743.
mla: Penttonen, Markku, et al. “Ultra-Slow Oscillation (0.025 Hz) Triggers Hippocampal
Afterdischarges in Wistar Rats.” Neuroscience, vol. 94, no. 3, Elsevier,
1999, pp. 735–43, doi:10.1016/S0306-4522(99)00367-X.
short: M. Penttonen, N. Nurminen, R. Miettinen, J. Sirviö, D. Henze, J.L. Csicsvari,
G. Buzsáki, Neuroscience 94 (1999) 735–743.
date_created: 2018-12-11T12:03:44Z
date_published: 1999-10-01T00:00:00Z
date_updated: 2022-09-07T13:16:01Z
day: '01'
doi: 10.1016/S0306-4522(99)00367-X
extern: '1'
external_id:
pmid:
- '10579564'
intvolume: ' 94'
issue: '3'
language:
- iso: eng
month: '10'
oa_version: None
page: 735 - 743
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '2870'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ultra-slow oscillation (0.025 Hz) triggers hippocampal afterdischarges in Wistar
rats
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 94
year: '1999'
...
---
_id: '3541'
abstract:
- lang: eng
text: 'The contribution of the various hippocampal regions to the maintenance of
epileptic activity, induced by stimulation of the perforant path or commissural
system, was examined in the awake rat. Combination of multiple-site recordings
with silicon probes, current source density analysis and unit recordings allowed
for a high spatial resolution of the field events. Following perforant path stimulation,
seizures began in the dentate gyrus, followed by events in the CA3-CA1 regions.
After commissural stimulation, rhythmic bursts in the CA3-CA1 circuitry preceded
the activation of the dentate gyrus. Correlation of events in the different subregions
indicated that the sustained rhythmic afterdischarge (2-6 Hz) could not be explained
by a cycle-by-cycle excitation of principal cell populations in the hippocampal-entorhinal
loop. The primary afterdischarge always terminated in the CA1 region, followed
by the dentate gyrus, CA3 region and the entorhinal cortex. The duration and pattern
of the hippocampal afterdischarge was essentially unaffected by removal of the
entorhinal cortex. The emergence of large population spike bursts coincided with
a decreased discharge of interneurons in both CAI and hilar regions. The majority
of hilar interneurons displayed a strong amplitude decrement prior to the onset
of population spike phase of the afterdischarge. These findings suggest that (i)
afterdischarges can independently arise in the CA3-CA1 and entorhinal-dentate
gyrus circuitries, (ii) reverberation of excitation in the hippocampal-entorhinal
loop is not critical for the maintenance of afterdischarges and (iii) decreased
activity of the interneuronal network may release population bursting of principal
cells. '
acknowledgement: We thank K. Wise and J. Hetke for providing us the silicon probes,
J. J. Chrobak, S. L-W. Leung, G. G. Somjen and R. D. Traub for their comments on
the manuscript. This work was supported by NINDS (NS34994; 1P41RR09754; NS33310)
and the Whitehall Foundation. M. Penttonen was a visiting scholar at Rutgers University,
supported by the Finnish Academy of Sciences and the A. I. Virtanen Institute.
article_processing_charge: No
article_type: original
author:
- first_name: Anatol
full_name: Bragin, Anatol
last_name: Bragin
- first_name: Jozsef L
full_name: Csicsvari, Jozsef L
id: 3FA14672-F248-11E8-B48F-1D18A9856A87
last_name: Csicsvari
orcid: 0000-0002-5193-4036
- first_name: Markku
full_name: Penttonen, Markku
last_name: Penttonen
- first_name: György
full_name: Buzsáki, György
last_name: Buzsáki
citation:
ama: 'Bragin A, Csicsvari JL, Penttonen M, Buzsáki G. Epileptic afterdischarge in
the hippocampal-entorhinal system: Current source density and unit studies. Neuroscience.
1997;76(4):1187-1203. doi:10.1016/S0306-4522(96)00446-0'
apa: 'Bragin, A., Csicsvari, J. L., Penttonen, M., & Buzsáki, G. (1997). Epileptic
afterdischarge in the hippocampal-entorhinal system: Current source density and
unit studies. Neuroscience. Elsevier. https://doi.org/10.1016/S0306-4522(96)00446-0'
chicago: 'Bragin, Anatol, Jozsef L Csicsvari, Markku Penttonen, and György Buzsáki.
“Epileptic Afterdischarge in the Hippocampal-Entorhinal System: Current Source
Density and Unit Studies.” Neuroscience. Elsevier, 1997. https://doi.org/10.1016/S0306-4522(96)00446-0.'
ieee: 'A. Bragin, J. L. Csicsvari, M. Penttonen, and G. Buzsáki, “Epileptic afterdischarge
in the hippocampal-entorhinal system: Current source density and unit studies,”
Neuroscience, vol. 76, no. 4. Elsevier, pp. 1187–1203, 1997.'
ista: 'Bragin A, Csicsvari JL, Penttonen M, Buzsáki G. 1997. Epileptic afterdischarge
in the hippocampal-entorhinal system: Current source density and unit studies.
Neuroscience. 76(4), 1187–1203.'
mla: 'Bragin, Anatol, et al. “Epileptic Afterdischarge in the Hippocampal-Entorhinal
System: Current Source Density and Unit Studies.” Neuroscience, vol. 76,
no. 4, Elsevier, 1997, pp. 1187–203, doi:10.1016/S0306-4522(96)00446-0.'
short: A. Bragin, J.L. Csicsvari, M. Penttonen, G. Buzsáki, Neuroscience 76 (1997)
1187–1203.
date_created: 2018-12-11T12:03:52Z
date_published: 1997-01-15T00:00:00Z
date_updated: 2022-08-19T11:53:06Z
day: '15'
doi: 10.1016/S0306-4522(96)00446-0
extern: '1'
external_id:
pmid:
- '9027878'
intvolume: ' 76'
issue: '4'
language:
- iso: eng
month: '01'
oa_version: None
page: 1187 - 1203
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '2844'
quality_controlled: '1'
status: public
title: 'Epileptic afterdischarge in the hippocampal-entorhinal system: Current source
density and unit studies'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 76
year: '1997'
...
---
_id: '2492'
abstract:
- lang: eng
text: The metabotropic glutamate receptor subtypes mGluR2 and mGluR5, which are
thought to be coupled respectively to the inhibitory cyclic adenosine monophosphate
(cAMP) cascade and the phosphatidylinositol hydrolysis/Ca2+ cascade, are known
to be expressed on Golgi cells in the granular layer of the rat cerebellar cortex.
In the present immunohistochemical study with a monoclonal antibody against mGluR2
and a polyclonal antibody for mGluR5, we examined whether or not mGluR2- and mGluR5-like
immunoreactivities were both present in single Golgi cells in the rat cerebellar
cortex. In double immunofluorescence histochemistry, no Golgi cells showed mGluR2-
and mGluR5-like immunoreactivities simultaneously. Of the total number of Golgi
cells immunoreactive for mGluR2 or mGluR5, about 90% were mGluR2-like immunoreactive,
and about 10% were mGluR5-like immunoreactive. Golgi cells with mGluR2-like immunoreactivity
were distributed evenly in the granular layer of all the cerebellar regions, while
those with mGluR5-like immunoreactivity were distributed more frequently in the
I, II, VII-X lobules of the vermis and the copula pyramidis of the hemisphere
than in other cerebellar regions. The results indicate that Golgi cells containing
mGluR2 are segregated from those possessing mGluR5. These two populations of Golgi
cells, each equipped with a different metabolic glutamate receptor coupled to
a different intracellular signal transduction system, may play different roles
in the glutamatergic neuronal circuits in the cerebellar cortex.
acknowledgement: We thank Mr Akira Uesugi for expert photographic assistance. We also
thank Dr Jeremy M. Henley for a critical reading of the manuscript.
article_processing_charge: No
article_type: original
author:
- first_name: Akio
full_name: Neki, Akio
last_name: Neki
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Neki A, Ohishi H, Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. Metabotropic
glutamate receptors mGluR2 and mGluR5 are expressed in two non-overlapping populations
of Golgi cells in the rat cerebellum. Neuroscience. 1996;75(3):815-826.
doi:10.1016/0306-4522(96)00316-8
apa: Neki, A., Ohishi, H., Kaneko, T., Shigemoto, R., Nakanishi, S., & Mizuno,
N. (1996). Metabotropic glutamate receptors mGluR2 and mGluR5 are expressed in
two non-overlapping populations of Golgi cells in the rat cerebellum. Neuroscience.
Elsevier. https://doi.org/10.1016/0306-4522(96)00316-8
chicago: Neki, Akio, Hitoshi Ohishi, Takeshi Kaneko, Ryuichi Shigemoto, Shigetada
Nakanishi, and Noboru Mizuno. “Metabotropic Glutamate Receptors MGluR2 and MGluR5
Are Expressed in Two Non-Overlapping Populations of Golgi Cells in the Rat Cerebellum.”
Neuroscience. Elsevier, 1996. https://doi.org/10.1016/0306-4522(96)00316-8.
ieee: A. Neki, H. Ohishi, T. Kaneko, R. Shigemoto, S. Nakanishi, and N. Mizuno,
“Metabotropic glutamate receptors mGluR2 and mGluR5 are expressed in two non-overlapping
populations of Golgi cells in the rat cerebellum,” Neuroscience, vol. 75,
no. 3. Elsevier, pp. 815–826, 1996.
ista: Neki A, Ohishi H, Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. 1996. Metabotropic
glutamate receptors mGluR2 and mGluR5 are expressed in two non-overlapping populations
of Golgi cells in the rat cerebellum. Neuroscience. 75(3), 815–826.
mla: Neki, Akio, et al. “Metabotropic Glutamate Receptors MGluR2 and MGluR5 Are
Expressed in Two Non-Overlapping Populations of Golgi Cells in the Rat Cerebellum.”
Neuroscience, vol. 75, no. 3, Elsevier, 1996, pp. 815–26, doi:10.1016/0306-4522(96)00316-8.
short: A. Neki, H. Ohishi, T. Kaneko, R. Shigemoto, S. Nakanishi, N. Mizuno, Neuroscience
75 (1996) 815–826.
date_created: 2018-12-11T11:57:59Z
date_published: 1996-12-01T00:00:00Z
date_updated: 2022-08-12T12:11:03Z
day: '01'
doi: 10.1016/0306-4522(96)00316-8
extern: '1'
external_id:
pmid:
- '8951875'
intvolume: ' 75'
issue: '3'
language:
- iso: eng
month: '12'
oa_version: None
page: 815 - 826
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '4409'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Metabotropic glutamate receptors mGluR2 and mGluR5 are expressed in two non-overlapping
populations of Golgi cells in the rat cerebellum
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 75
year: '1996'
...
---
_id: '2488'
abstract:
- lang: eng
text: Substance P receptor-expressing neurons in the rat cerebral neocortex were
examined by single- and double-immunolabeling methods with an affinity-purified
specific antibody to substance P receptor. Substance P receptor immunoreactivity
was observed exclusively in non-pyramidal neurons. About a quarter of these substance
P receptor-positive neocortical neurons showed intense immunoreactivity, and the
other three quarters displayed weak substance P receptor immunoreactivity. The
neurons showing intense substance P receptor immunoreactivity were large multipolar
cells with a few long aspiny or sparsely-spiny dendrites, and were scattered throughout
the neocortical layers except for layer I, and also in the underlying white matter.
The weakly immunoreactive neurons were medium-sized multipolar cells with oval
to round somata and aspiny varicose dendrites, and were distributed in all cortical
layers with a bias to layers II-III and the superficial part of layer V. The double-immunofluorescence
study revealed that almost all substance P receptor-positive neurons were immunoreactive
for GABA, but negative for glutaminase. Substance P receptor immunoreactivity
in GABAergic neocortical neurons were further examined by the double-immunofluorescence
method with antibodies to markers for subgroups of GABAergic neurons. Somatostatin
immunoreactivity was found in 89% of neurons with intense substance P receptor
immunoreactivity, and in 1.5% of neurons with weak substance P receptor immunoreactivity.
Neuropeptide Y immunoreactivity was also observed in 92% of neurons with intense
immunoreactivity for substance P receptor, and in 1.6% of neurons with weak immunoreactivity
for substance P receptor. In contrast, parvalbumin immunoreactivity was seen in
1.3% of neurons with intense substance P receptor immunoreactivity, and in 59%
of weak substance P receptor immunoreactivity. Calbindin D28k immunoreactivity
was found in 12 and 19% of neurons, respectively, with weak and intense immunoreactivities
for substance P receptor. Virtually no cells showing substance P receptor immunoreactivity
displayed immunoreactivity for vasoactive intestinal polypeptide or choline acetyltransferase.
These results indicate that the neocortical neurons expressing substance P receptor
constitute a subpopulation of GABAergic non-pyramidal cells, and are segregated
into neurons with intense immunoreactivity and those with weak immunoreactivity
for substance P receptor; the vast majority of neurons with intense substance
P receptor immunoreactivity contain somatostatin and neuropeptide Y, and the majority
of neurons with weak substance P receptor immunoreactivity have parvalbumin.
acknowledgement: We are grateful for photographic help of Mr A. Uesugi, and the support
of Drs S. Fukuchi, T. Fukuda, R. Hayashi, M. Katsurada, Y. Kitani, K. Kumagai, H.
Kuroda, H. Matsubara, H. Matsushima, C. Minakuchi, M. Nishio, G. Niwa, H. Ckla,
M. Ohbayashi, S. Ohbayashi, H. Ohtsuka, S. Tamaki, E. Watanabe, K. Yoshino and Y.
Yoshino. This work was supported in part by Grants-in-Aid for Scientific Research
on Priority Areas 05248207 and 05267104, and Scientific Research (B) 05454658 and
(C) 05680658 from the Ministry of Education, Science and Culture of Japan.
article_processing_charge: No
article_type: original
author:
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. Morphological and chemical characteristics
of substance P receptor immunoreactive neurons in the rat neocortex. Neuroscience.
1994;60(1):199-211. doi:10.1016/0306-4522(94)90215-1
apa: Kaneko, T., Shigemoto, R., Nakanishi, S., & Mizuno, N. (1994). Morphological
and chemical characteristics of substance P receptor immunoreactive neurons in
the rat neocortex. Neuroscience. Elsevier. https://doi.org/10.1016/0306-4522(94)90215-1
chicago: Kaneko, Takeshi, Ryuichi Shigemoto, Shigetada Nakanishi, and Noboru Mizuno.
“Morphological and Chemical Characteristics of Substance P Receptor Immunoreactive
Neurons in the Rat Neocortex.” Neuroscience. Elsevier, 1994. https://doi.org/10.1016/0306-4522(94)90215-1.
ieee: T. Kaneko, R. Shigemoto, S. Nakanishi, and N. Mizuno, “Morphological and chemical
characteristics of substance P receptor immunoreactive neurons in the rat neocortex,”
Neuroscience, vol. 60, no. 1. Elsevier, pp. 199–211, 1994.
ista: Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. 1994. Morphological and chemical
characteristics of substance P receptor immunoreactive neurons in the rat neocortex.
Neuroscience. 60(1), 199–211.
mla: Kaneko, Takeshi, et al. “Morphological and Chemical Characteristics of Substance
P Receptor Immunoreactive Neurons in the Rat Neocortex.” Neuroscience,
vol. 60, no. 1, Elsevier, 1994, pp. 199–211, doi:10.1016/0306-4522(94)90215-1.
short: T. Kaneko, R. Shigemoto, S. Nakanishi, N. Mizuno, Neuroscience 60 (1994)
199–211.
date_created: 2018-12-11T11:57:58Z
date_published: 1994-05-01T00:00:00Z
date_updated: 2022-06-09T12:22:16Z
day: '01'
doi: 10.1016/0306-4522(94)90215-1
extern: '1'
external_id:
pmid:
- '8052413'
intvolume: ' 60'
issue: '1'
language:
- iso: eng
main_file_link:
- url: https://www.sciencedirect.com/science/article/pii/0306452294902151?via%3Dihub
month: '05'
oa_version: None
page: 199 - 211
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '4413'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Morphological and chemical characteristics of substance P receptor immunoreactive
neurons in the rat neocortex
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 60
year: '1994'
...
---
_id: '2490'
abstract:
- lang: eng
text: Distribution of the messenger RNA for the prostaglandin E receptor subtype
EP3 was investigated by in situ hybridization in the nervous system of the mouse.
The hybridization signals for EP3 were widely distributed in the brain and sensory
ganglia and specifically localized to neurons. In the dorsal root and trigeminal
ganglia, about half of the neurons were labeled intensely. In the brain, intensely
labeled neurons were found in Ammon's horn, the preoptic nuclei, lateral hypothalamic
area, dorsomedial hypothalamic nucleus, lateral mammillary nucleus, entopeduncular
nucleus, substantia nigra pars compacta, locus coeruleus and raphe nuclei. Moderately
labeled neurons were seen in the mitral cell layer of the main olfactory bulb,
layer V of the entorhinal and parasubicular cortices, layers V and VI of the cerebral
neocortex, nuclei of the diagonal band, magnocellular preoptic nucleus, globus
pallidus and lateral parabrachial nucleus. In the thalamus, moderately labeled
neurons were distributed in the anterior, ventromedial, laterodorsal, paraventricular
and central medial nuclei. Based on these distributions, we suggest that EP3 not
only mediates prostaglandin E2 signals evoked by blood-borne cytokines in the
areas poor in the blood-brain barrier, but also responds to those formed intrinsically
within the brain to modulate various neuronal activities. Possible EP3 actions
are discussed in relation to the reported neuronal activities of prostaglandin
E2 in the brain.
acknowledgement: 'This work was supported in part by Grants-in-aid for Scientific
Research 05404020, 04255103. 05771975, 05671816 and 05454568 from the Ministry of
Education, Science and Culture of Japan and by grants from the Mitsubishi Foundation
and the Takeda Science Foundation. We are grateful to Mr Akira Uesugi for photographic
help. We also thank Drs Chihiro Akazawa, Hitoshi Ohishi and Masabumi Minami for
helpful discussions. '
article_processing_charge: No
article_type: original
author:
- first_name: Yukihiko
full_name: Sugimoto, Yukihiko
last_name: Sugimoto
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Tsunehisa
full_name: Namba, Tsunehisa
last_name: Namba
- first_name: Manabu
full_name: Negishi, Manabu
last_name: Negishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
- first_name: Shuh
full_name: Narumiya, Shuh
last_name: Narumiya
- first_name: Atsushi
full_name: Ichikawa, Atsushi
last_name: Ichikawa
citation:
ama: Sugimoto Y, Shigemoto R, Namba T, et al. Distribution of the messenger rna
for the prostaglandin e receptor subtype ep3 in the mouse nervous system. Neuroscience.
1994;62(3):919-928. doi:10.1016/0306-4522(94)90483-9
apa: Sugimoto, Y., Shigemoto, R., Namba, T., Negishi, M., Mizuno, N., Narumiya,
S., & Ichikawa, A. (1994). Distribution of the messenger rna for the prostaglandin
e receptor subtype ep3 in the mouse nervous system. Neuroscience. Elsevier.
https://doi.org/10.1016/0306-4522(94)90483-9
chicago: Sugimoto, Yukihiko, Ryuichi Shigemoto, Tsunehisa Namba, Manabu Negishi,
Noboru Mizuno, Shuh Narumiya, and Atsushi Ichikawa. “Distribution of the Messenger
Rna for the Prostaglandin e Receptor Subtype Ep3 in the Mouse Nervous System.”
Neuroscience. Elsevier, 1994. https://doi.org/10.1016/0306-4522(94)90483-9.
ieee: Y. Sugimoto et al., “Distribution of the messenger rna for the prostaglandin
e receptor subtype ep3 in the mouse nervous system,” Neuroscience, vol.
62, no. 3. Elsevier, pp. 919–928, 1994.
ista: Sugimoto Y, Shigemoto R, Namba T, Negishi M, Mizuno N, Narumiya S, Ichikawa
A. 1994. Distribution of the messenger rna for the prostaglandin e receptor subtype
ep3 in the mouse nervous system. Neuroscience. 62(3), 919–928.
mla: Sugimoto, Yukihiko, et al. “Distribution of the Messenger Rna for the Prostaglandin
e Receptor Subtype Ep3 in the Mouse Nervous System.” Neuroscience, vol.
62, no. 3, Elsevier, 1994, pp. 919–28, doi:10.1016/0306-4522(94)90483-9.
short: Y. Sugimoto, R. Shigemoto, T. Namba, M. Negishi, N. Mizuno, S. Narumiya,
A. Ichikawa, Neuroscience 62 (1994) 919–928.
date_created: 2018-12-11T11:57:58Z
date_published: 1994-10-01T00:00:00Z
date_updated: 2022-06-09T11:56:23Z
day: '01'
doi: 10.1016/0306-4522(94)90483-9
extern: '1'
external_id:
pmid:
- '7870313'
intvolume: ' 62'
issue: '3'
language:
- iso: eng
main_file_link:
- url: https://www.sciencedirect.com/science/article/pii/0306452294904839?via%3Dihub
month: '10'
oa_version: None
page: 919 - 928
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '4411'
quality_controlled: '1'
status: public
title: Distribution of the messenger rna for the prostaglandin e receptor subtype
ep3 in the mouse nervous system
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 62
year: '1994'
...
---
_id: '2540'
abstract:
- lang: eng
text: Distribution of the messenger RNA for a metabotropic glutamate receptor, mGluR2,
which is coupled to the inhibitory cyclic AMP cascade, was investigated in the
central nervous system of the adult rat by in situ hybridization. Transcripts
of mGluR2 were specifically localized to neuronal cells of the brain. Although
the hybridization signals were widely distributed in the brain, the most prominent
expression of mGluR2 messenger RNA was seen in Golgi cells of the cerebellum.
Marked expression of mGluR2 messenger RNA was further observed in the mitral cells
of the accessory olfactory bulb, neurons in the external part of the anterior
olfactory nucleus, and pyramidal neurons in the entorhinal and parasubicular cortical
regions. The granule cells of the accessory olfactory bulb, and many pyramidal
and non-pyramidal neurons in the neocortical, cingulate, retrosplenial and subicular
cortices, were moderately labeled. All of the granule cells in the dentate gyrus
were also labeled moderately, whereas no significant hybridization signals were
detected in Ammon's horn. In the basal forebrain regions, moderately labeled neurons
were distributed in the triangular septal nucleus, in the lateral, basolateral
and basomedial amygdaloid nuclei, and in the medial mammillary nucleus. Weakly
labeled neurons were sparsely scattered in the striatum, globus pallidus, ventral
pallidum and claustrum. The subthalamic nucleus was also labeled weakly. No significant
labeling was found in the entopeduncular nucleus and substantia nigra. In the
thalamus, moderately labeled neurons were distributed in the anterodorsal, anteromedial,
ventromedial, intralaminar and midline nuclei; the ventrolateral part of the anteroventral
nucleus and the rostral pole of the ventrolateral nucleus also contained moderately
labeled neurons. No significant labeling was found in the thalamic reticular,
submedius, ventroposterior, lateral geniculate and medial geniculate nuclei. In
the lower brainstem, labeling was generally weak. No significant hybridization
signals were found in the spinal cord. Some neurons in the inner part of the inner
nuclear layer of the retina and some retinal ganglion cells were labeled moderately.
The pattern of distribution of mGluR2 messenger RNA revealed in the present study
indicates specific roles of mGluR2 in the glutamatergic system in the brain.
acknowledgement: We are grateful for the photographic help of Mr Akira Uesugi and
the support of Drs Ryosuke Fujimori, Satoru Fukuchi, Toshio Fukuda, Ritsu Hayashi,
Sozaburo Hayashi, Mizuho Katsurada, Yutaka Kitani, Keiko Kumagai, Hiroshi Kuroda,
Toshio Kuroda, Hiroshi Matsubara. Hiroshi Matsushima. Chisato Minakuchi. Masatoshi
‘Nishio, Gonpei Niwa, Hajime Oda, Masahiko Ohbayashi, Seiichi Ohbayashi, Hiroyasu
Ohtsuka, Shigeo Tamaki, Eizo Watanabe, Kazuo Yoshino and Toshiaki Yoshino. This
work was supported in part by Grants-in-Aid from the Ministry of Education, Science
and Culture of Japan.
article_processing_charge: No
article_type: original
author:
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Ohishi H, Shigemoto R, Nakanishi S, Mizuno N. Distribution of the messenger
RNA for a metabotropic glutamate receptor, mGluR2, in the central nervous system
of the rat. Neuroscience. 1993;53(4):1009-1018. doi:10.1016/0306-4522(93)90485-X
apa: Ohishi, H., Shigemoto, R., Nakanishi, S., & Mizuno, N. (1993). Distribution
of the messenger RNA for a metabotropic glutamate receptor, mGluR2, in the central
nervous system of the rat. Neuroscience. Elsevier. https://doi.org/10.1016/0306-4522(93)90485-X
chicago: Ohishi, Hitoshi, Ryuichi Shigemoto, Shigetada Nakanishi, and Noboru Mizuno.
“Distribution of the Messenger RNA for a Metabotropic Glutamate Receptor, MGluR2,
in the Central Nervous System of the Rat.” Neuroscience. Elsevier, 1993.
https://doi.org/10.1016/0306-4522(93)90485-X.
ieee: H. Ohishi, R. Shigemoto, S. Nakanishi, and N. Mizuno, “Distribution of the
messenger RNA for a metabotropic glutamate receptor, mGluR2, in the central nervous
system of the rat,” Neuroscience, vol. 53, no. 4. Elsevier, pp. 1009–1018,
1993.
ista: Ohishi H, Shigemoto R, Nakanishi S, Mizuno N. 1993. Distribution of the messenger
RNA for a metabotropic glutamate receptor, mGluR2, in the central nervous system
of the rat. Neuroscience. 53(4), 1009–1018.
mla: Ohishi, Hitoshi, et al. “Distribution of the Messenger RNA for a Metabotropic
Glutamate Receptor, MGluR2, in the Central Nervous System of the Rat.” Neuroscience,
vol. 53, no. 4, Elsevier, 1993, pp. 1009–18, doi:10.1016/0306-4522(93)90485-X.
short: H. Ohishi, R. Shigemoto, S. Nakanishi, N. Mizuno, Neuroscience 53 (1993)
1009–1018.
date_created: 2018-12-11T11:58:16Z
date_published: 1993-01-01T00:00:00Z
date_updated: 2022-03-31T12:19:44Z
day: '01'
doi: 10.1016/0306-4522(93)90485-X
extern: '1'
external_id:
pmid:
- '8389425'
intvolume: ' 53'
issue: '4'
language:
- iso: eng
main_file_link:
- url: https://www.sciencedirect.com/science/article/pii/030645229390485X?via%3Dihub
month: '01'
oa_version: None
page: 1009 - 1018
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '4358'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distribution of the messenger RNA for a metabotropic glutamate receptor, mGluR2,
in the central nervous system of the rat
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 53
year: '1993'
...
---
_id: '2479'
abstract:
- lang: eng
text: Distribution of putative glutamatergic neurons in the lower brainstem and
cerebellum of the rat was examined immunocytochemically by using a monoclonal
antibody against phosphate-activated glutaminase, which has been proposed to be
a major synthetic enzyme of transmitter glutamate and so may serve as a marker
for glutamatergic neurons in the central nervous system. Intensely-immunolabeled
neuronal cell bodies were densely distributed in the main precerebellar nuclei
sending mossy fibers to the cerebellum; in the pontine nuclei, pontine tegmental
reticular nucleus of Bechterew, external cuneate nucleus, and lateral reticular
nucleus of the medulla oblongata. Phosphate-activated glutaminase-immunoreactive
granular deposits were densely seen in the brachium pontis and restiform body,
suggesting the immunolabeling of mossy fibers of passage. In the cerebellum, neuropil
within the granule cell layer of the cerebellar cortex displayed intense phosphate-activated
glutaminase-immunoreactivity, and that within the deep cerebellar nuclei showed
moderate immunoreactivity. These results indicate that many mossy fiber terminals
originate from phosphate-activated glutaminase-containing neurons and utilize
phosphate-activated glutaminase for the synthesis of transmitter glutamate. Intensely-immunostained
neuronal cell bodies were further observed in other regions which have been reported
to contain neurons sending mossy fibers to the cerebellum; in the dorsal part
of the principal sensory trigeminal nucleus, dorsomedial part of the oral subnucleus
of the spinal trigeminal nucleus, interpolar subnucleus of the spinal trigeminal
nucleus, paratrigeminal nucleus, supragenual nucleus, regions dorsal to the abducens
nucleus and genu of the facial nerve, superior and medial vestibular nuclei, cell
groups f, x and y, hypoglossal prepositus nucleus, intercalated nucleus, nucleus
of Roller, reticular regions intercalated between the motor trigeminal and principal
sensory trigeminal nuclei, linear nucleus, and gigantocellular and paramedian
reticular formation. Neuronal cell bodies with intense phosphate-activated glutaminase-immunoreactivity
were also found in other brainstem regions, such as the paracochlear glial substance,
posterior ventral cochlear nucleus, and cell group e. Although it is still controversial
whether all glutamatergic neurons use phosphate-activated glutaminase in a transmitter-related
process and whether phosphate-activated glutaminase is involved in other metabolism-related
processes, the neurons showing intense phosphate-activated glutaminase-immuno-reactivity
in the present study were suggested to be putative glutamatergic neurons.
acknowledgement: 'The authors wish to thank Mr. Akira Uesugi and Mr. Ken’ichi Uesugi
for their photographic help. This work was partly supported by grants-in-aid from
the Ministry of Education, science and Culture of Japan for Special Project Research
63112003. Special Research Project on Priority-Areas 63623505, Special Research
62480098 and Encouragement of Young Scientist 63770043. The support of the Niwa
Medical Research Foundation, Dr. Satoru Fukuchi, Dr. Toshio Fukuda, Dr. Ritsu Hayashi,
Dr. Yutaka Kitani, Dr. Hiroshi Matsushima, Dr. Gonpei Niwa, Dr. Hiroyasu Ohtsuka,
Dr. Shigeo Tamaki, and Dr. Eizo Watanabe are gratefully acknowledged. '
article_processing_charge: No
article_type: original
author:
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Kazuo
full_name: Itoh, Kazuo
last_name: Itoh
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Kaneko T, Itoh K, Shigemoto R, Mizuno N. Glutaminase-like immunoreactivity
in the lower brainstem and cerebellum of the adult rat. Neuroscience. 1989;32(1):79-98.
doi:10.1016/0306-4522(89)90109-7
apa: Kaneko, T., Itoh, K., Shigemoto, R., & Mizuno, N. (1989). Glutaminase-like
immunoreactivity in the lower brainstem and cerebellum of the adult rat. Neuroscience.
Elsevier. https://doi.org/10.1016/0306-4522(89)90109-7
chicago: Kaneko, Takeshi, Kazuo Itoh, Ryuichi Shigemoto, and Noboru Mizuno. “Glutaminase-like
Immunoreactivity in the Lower Brainstem and Cerebellum of the Adult Rat.” Neuroscience.
Elsevier, 1989. https://doi.org/10.1016/0306-4522(89)90109-7.
ieee: T. Kaneko, K. Itoh, R. Shigemoto, and N. Mizuno, “Glutaminase-like immunoreactivity
in the lower brainstem and cerebellum of the adult rat,” Neuroscience,
vol. 32, no. 1. Elsevier, pp. 79–98, 1989.
ista: Kaneko T, Itoh K, Shigemoto R, Mizuno N. 1989. Glutaminase-like immunoreactivity
in the lower brainstem and cerebellum of the adult rat. Neuroscience. 32(1), 79–98.
mla: Kaneko, Takeshi, et al. “Glutaminase-like Immunoreactivity in the Lower Brainstem
and Cerebellum of the Adult Rat.” Neuroscience, vol. 32, no. 1, Elsevier,
1989, pp. 79–98, doi:10.1016/0306-4522(89)90109-7.
short: T. Kaneko, K. Itoh, R. Shigemoto, N. Mizuno, Neuroscience 32 (1989) 79–98.
date_created: 2018-12-11T11:57:54Z
date_published: 1989-01-01T00:00:00Z
date_updated: 2022-02-15T09:47:08Z
day: '01'
doi: 10.1016/0306-4522(89)90109-7
extern: '1'
external_id:
pmid:
- '2586753'
intvolume: ' 32'
issue: '1'
language:
- iso: eng
main_file_link:
- url: https://www.sciencedirect.com/science/article/pii/0306452289901097?via%3Dihub
month: '01'
oa_version: None
page: 79 - 98
pmid: 1
publication: Neuroscience
publication_identifier:
eissn:
- 1873-7544
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
publist_id: '4422'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Glutaminase-like immunoreactivity in the lower brainstem and cerebellum of
the adult rat
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 32
year: '1989'
...