---
_id: '2590'
abstract:
- lang: eng
text: Unipolar brush cells (UBCs) are a class of small neurons that are densely
concentrated in the granular layers of the vestibulocerebellar cortex and dorsal
cochlear nucleus. The UBCs form giant synapses with individual mossy fibre rosettes
on the dendrioles which make up their brush formations and are provided with numerous,
unusual non-synaptic appendages. In accord with the glutamatergic nature of mossy
fibres, our previous post-embedding immunocytochemical studies indicated that
various ionotropic glutamate receptor subunits are localized at the post-synaptic
densities of the giant synapses, whereas the non-synaptic appendages are immunonegative.
On the contrary, the metabotropic glutamate receptors mGluR1α and mGluR2/3 are
situated at the non-synaptic appendages and are lacking at the post-synaptic densities.
Other authors, however, have shown that antibodies to these metabotropic receptors
stain both appendages and post-synaptic densities. In the present study, we have
re-evaluated the distribution of metabotropic glutamate receptors in the UBCs
of the cerebellum and the cochlear nuclear complex by light and electron microscopic
pre-embedding immunocytochemistry with subtype-specific antibodies. We confirm
that UBCs dendritic brushes are densely immunostained by antibody to mGluR1α particularly
in the cerebellum and that antibody to mGluR2/3 labels at least a percentage of
the UBC brushes in both the cerebellum and cochlear nuclei. At the ultrastructural
level, it appears that mGluR1α and mGluR2/3 immunoreactivities are not associated
with the post-synaptic densities of the giant mossy fibre-UBC synapses, but instead
are concentrated on the non-synaptic appendages of the cerebellar UBCs. The non-synaptic
appendages, therefore, may be an important avenue for regulating the excitability
of UBCs and mediating glutamate effects on their still unknown intracellular signal
transduction cascades. We also show that the pre-synaptic densities of UBC dendrodendritic
junctions are mGluR2/3 positive. As previously demonstrated, antibodies to mGluR1α
and mGluR2/3 label subsets of Golgi cells. Antibody to mGluR5 does not stain UBCs
in the cerebellum and cochlear nucleus and reveals the somatodendritic compartment
of Golgi cells situated in the core of the cerebellar granular layer, whilst cochlear
nucleus Golgi cells are mGluR5 negative.
acknowledgement: The authors wish to thank Dr R. L. Huganir and coworkers for kindly
providing an aliquot of their mGluR1a antibody and Dr N. Traverse Slater for helpful
comments on the manuscript. The study was supported by US-PHS grants NS 09904 and
DC 01805 (to E.M.).
article_processing_charge: No
article_type: original
author:
- first_name: Dick
full_name: Jaarsma, Dick
last_name: Jaarsma
- first_name: Maria
full_name: Diño, Maria
last_name: Diño
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Enrico
full_name: Mugnaini, Enrico
last_name: Mugnaini
citation:
ama: Jaarsma D, Diño M, Ohishi H, Shigemoto R, Mugnaini E. Metabotropic glutamate
receptors are associated with non-synaptic appendages of unipolar brush cells
in rat cerebellar cortex and cochlear nuclear complex. Journal of Neurocytology.
1998;27(5):303-327. doi:10.1023/A:1006982023657
apa: Jaarsma, D., Diño, M., Ohishi, H., Shigemoto, R., & Mugnaini, E. (1998). Metabotropic
glutamate receptors are associated with non-synaptic appendages of unipolar brush
cells in rat cerebellar cortex and cochlear nuclear complex. Journal of Neurocytology.
Kluwer. https://doi.org/10.1023/A:1006982023657
chicago: Jaarsma, Dick, Maria Diño, Hitoshi Ohishi, Ryuichi Shigemoto, and Enrico
Mugnaini. “ Metabotropic Glutamate Receptors Are Associated with Non-Synaptic
Appendages of Unipolar Brush Cells in Rat Cerebellar Cortex and Cochlear Nuclear
Complex.” Journal of Neurocytology. Kluwer, 1998. https://doi.org/10.1023/A:1006982023657.
ieee: D. Jaarsma, M. Diño, H. Ohishi, R. Shigemoto, and E. Mugnaini, “ Metabotropic
glutamate receptors are associated with non-synaptic appendages of unipolar brush
cells in rat cerebellar cortex and cochlear nuclear complex,” Journal of Neurocytology,
vol. 27, no. 5. Kluwer, pp. 303–327, 1998.
ista: Jaarsma D, Diño M, Ohishi H, Shigemoto R, Mugnaini E. 1998. Metabotropic
glutamate receptors are associated with non-synaptic appendages of unipolar brush
cells in rat cerebellar cortex and cochlear nuclear complex. Journal of Neurocytology.
27(5), 303–327.
mla: Jaarsma, Dick, et al. “ Metabotropic Glutamate Receptors Are Associated with
Non-Synaptic Appendages of Unipolar Brush Cells in Rat Cerebellar Cortex and Cochlear
Nuclear Complex.” Journal of Neurocytology, vol. 27, no. 5, Kluwer, 1998,
pp. 303–27, doi:10.1023/A:1006982023657.
short: D. Jaarsma, M. Diño, H. Ohishi, R. Shigemoto, E. Mugnaini, Journal of Neurocytology
27 (1998) 303–327.
date_created: 2018-12-11T11:58:33Z
date_published: 1998-01-01T00:00:00Z
date_updated: 2022-08-31T12:30:14Z
day: '01'
doi: 10.1023/A:1006982023657
extern: '1'
external_id:
pmid:
- '9923978 '
intvolume: ' 27'
issue: '5'
language:
- iso: eng
month: '01'
oa_version: None
page: 303 - 327
pmid: 1
publication: Journal of Neurocytology
publication_identifier:
issn:
- 0300-4864
publication_status: published
publisher: Kluwer
publist_id: '4308'
quality_controlled: '1'
scopus_import: '1'
status: public
title: ' Metabotropic glutamate receptors are associated with non-synaptic appendages
of unipolar brush cells in rat cerebellar cortex and cochlear nuclear complex'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 27
year: '1998'
...