[{"article_processing_charge":"Yes (via OA deal)","scopus_import":"1","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"article_type":"original","publication":"EMBO Journal","pmid":1,"department":[{"_id":"NanoFab"},{"_id":"Bio"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Embo Press","title":"Adaptive pathfinding by nucleokinesis during amoeboid migration","article_number":"e114557","day":"21","file":[{"success":1,"file_name":"2023_EmboJournal_Kroll.pdf","relation":"main_file","content_type":"application/pdf","file_size":4862497,"creator":"dernst","date_updated":"2023-11-27T08:45:56Z","file_id":"14611","checksum":"6261d0041c7e8d284c39712c40079730","date_created":"2023-11-27T08:45:56Z","access_level":"open_access"}],"author":[{"full_name":"Kroll, Janina","first_name":"Janina","last_name":"Kroll"},{"last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522"},{"first_name":"Arthur","last_name":"Kuznetcov","full_name":"Kuznetcov, Arthur"},{"full_name":"Stefanowski, Kasia","first_name":"Kasia","last_name":"Stefanowski"},{"first_name":"Monika D.","last_name":"Hermann","full_name":"Hermann, Monika D."},{"full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","first_name":"Jack","last_name":"Merrin"},{"last_name":"Shafeek","first_name":"Lubuna B","full_name":"Shafeek, Lubuna B","orcid":"0000-0001-7180-6050","id":"3CD37A82-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Annette","last_name":"Müller-Taubenberger","full_name":"Müller-Taubenberger, Annette"},{"last_name":"Renkawitz","first_name":"Jörg","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2856-3369","full_name":"Renkawitz, Jörg"}],"language":[{"iso":"eng"}],"quality_controlled":"1","doi":"10.15252/embj.2023114557","publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"_id":"13342","year":"2023","acknowledgement":"We thank Christoph Mayr and Bingzhi Wang for initial experiments on amoeboid nucleokinesis, Ana-Maria Lennon-Duménil and Aline Yatim for bone marrow from MyoIIA-Flox*CD11c-Cre mice, Michael Sixt and Aglaja Kopf for EMTB-mCherry, EB3-mCherry, Lifeact-GFP, Lfc knockout, and Myh9-GFP expressing HoxB8 cells, Malte Benjamin Braun, Mauricio Ruiz, and Madeleine T. Schmitt for critical reading of the manuscript, and the Core Facility Bioimaging, the Core Facility Flow Cytometry, and the Animal Core Facility of the Biomedical Center (BMC) for excellent support. This study was supported by the Peter Hans Hofschneider Professorship of the foundation “Stiftung Experimentelle Biomedizin” (to JR), the LMU Institutional Strategy LMU-Excellent within the framework of the German Excellence Initiative (to JR), and the Deutsche Forschungsgemeinschaft (DFG; German Research Foundation; SFB914 project A12, to JR), and the CZI grant DAF2020-225401 (https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF (to RH; an advised fund of Silicon Valley Community Foundation (funder https://doi.org/10.13039/100014989)). Open Access funding enabled and organized by Projekt DEAL.","file_date_updated":"2023-11-27T08:45:56Z","date_created":"2023-08-01T08:59:06Z","date_updated":"2023-11-27T08:47:45Z","abstract":[{"text":"Motile cells moving in multicellular organisms encounter microenvironments of locally heterogeneous mechanochemical composition. Individual compositional parameters like chemotactic signals, adhesiveness, and pore sizes are well known to be sensed by motile cells, providing individual guidance cues for cellular pathfinding. However, motile cells encounter diverse mechanochemical signals at the same time, raising the question of how cells respond to locally diverse and potentially competing signals on their migration routes. Here, we reveal that motile amoeboid cells require nuclear repositioning, termed nucleokinesis, for adaptive pathfinding in heterogeneous mechanochemical microenvironments. Using mammalian immune cells and the amoeba<jats:italic>Dictyostelium discoideum</jats:italic>, we discover that frequent, rapid and long-distance nucleokinesis is a basic component of amoeboid pathfinding, enabling cells to reorientate quickly between locally competing cues. Amoeboid nucleokinesis comprises a two-step cell polarity switch and is driven by myosin II-forces, sliding the nucleus from a ‘losing’ to the ‘winning’ leading edge to re-adjust the nuclear to the cellular path. Impaired nucleokinesis distorts fast path adaptions and causes cellular arrest in the microenvironment. Our findings establish that nucleokinesis is required for amoeboid cell navigation. Given that motile single-cell amoebae, many immune cells, and some cancer cells utilize an amoeboid migration strategy, these results suggest that amoeboid nucleokinesis underlies cellular navigation during unicellular biology, immunity, and disease.","lang":"eng"}],"type":"journal_article","oa_version":"Published Version","month":"11","citation":{"short":"J. Kroll, R. Hauschild, A. Kuznetcov, K. Stefanowski, M.D. Hermann, J. Merrin, L.B. Shafeek, A. Müller-Taubenberger, J. Renkawitz, EMBO Journal (2023).","ieee":"J. Kroll <i>et al.</i>, “Adaptive pathfinding by nucleokinesis during amoeboid migration,” <i>EMBO Journal</i>. Embo Press, 2023.","chicago":"Kroll, Janina, Robert Hauschild, Arthur Kuznetcov, Kasia Stefanowski, Monika D. Hermann, Jack Merrin, Lubuna B Shafeek, Annette Müller-Taubenberger, and Jörg Renkawitz. “Adaptive Pathfinding by Nucleokinesis during Amoeboid Migration.” <i>EMBO Journal</i>. Embo Press, 2023. <a href=\"https://doi.org/10.15252/embj.2023114557\">https://doi.org/10.15252/embj.2023114557</a>.","mla":"Kroll, Janina, et al. “Adaptive Pathfinding by Nucleokinesis during Amoeboid Migration.” <i>EMBO Journal</i>, e114557, Embo Press, 2023, doi:<a href=\"https://doi.org/10.15252/embj.2023114557\">10.15252/embj.2023114557</a>.","ista":"Kroll J, Hauschild R, Kuznetcov A, Stefanowski K, Hermann MD, Merrin J, Shafeek LB, Müller-Taubenberger A, Renkawitz J. 2023. Adaptive pathfinding by nucleokinesis during amoeboid migration. EMBO Journal., e114557.","apa":"Kroll, J., Hauschild, R., Kuznetcov, A., Stefanowski, K., Hermann, M. D., Merrin, J., … Renkawitz, J. (2023). Adaptive pathfinding by nucleokinesis during amoeboid migration. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2023114557\">https://doi.org/10.15252/embj.2023114557</a>","ama":"Kroll J, Hauschild R, Kuznetcov A, et al. Adaptive pathfinding by nucleokinesis during amoeboid migration. <i>EMBO Journal</i>. 2023. doi:<a href=\"https://doi.org/10.15252/embj.2023114557\">10.15252/embj.2023114557</a>"},"status":"public","external_id":{"pmid":["37987147"]},"ddc":["570"],"date_published":"2023-11-21T00:00:00Z","has_accepted_license":"1","oa":1,"publication_status":"published"},{"publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]},"doi":"10.15252/embj.2021108714","quality_controlled":"1","isi":1,"language":[{"iso":"eng"}],"issue":"23","author":[{"full_name":"Bajaj, Sunanjay","last_name":"Bajaj","first_name":"Sunanjay"},{"full_name":"Bagley, Joshua A.","last_name":"Bagley","first_name":"Joshua A."},{"first_name":"Christoph M","last_name":"Sommer","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M"},{"full_name":"Vertesy, Abel","first_name":"Abel","last_name":"Vertesy"},{"full_name":"Nagumo Wong, Sakurako","first_name":"Sakurako","last_name":"Nagumo Wong"},{"full_name":"Krenn, Veronica","last_name":"Krenn","first_name":"Veronica"},{"last_name":"Lévi-Strauss","first_name":"Julie","full_name":"Lévi-Strauss, Julie"},{"full_name":"Knoblich, Juergen A.","last_name":"Knoblich","first_name":"Juergen A."}],"file":[{"file_id":"10541","date_updated":"2021-12-13T14:54:14Z","checksum":"78d2d02e775322297e774f72810a41a4","date_created":"2021-12-13T14:54:14Z","access_level":"open_access","file_name":"2021_EMBO_Bajaj.pdf","success":1,"file_size":7819881,"content_type":"application/pdf","relation":"main_file","creator":"alisjak"}],"day":"18","title":"Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration","article_number":"e108714","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"Embo Press","pmid":1,"department":[{"_id":"Bio"}],"publication":"EMBO Journal","article_processing_charge":"Yes (in subscription journal)","scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","oa":1,"publication_status":"published","has_accepted_license":"1","ddc":["610"],"date_published":"2021-10-18T00:00:00Z","external_id":{"pmid":["34661293"],"isi":["000708012800001"]},"status":"public","intvolume":"        40","citation":{"ieee":"S. Bajaj <i>et al.</i>, “Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration,” <i>EMBO Journal</i>, vol. 40, no. 23. Embo Press, 2021.","chicago":"Bajaj, Sunanjay, Joshua A. Bagley, Christoph M Sommer, Abel Vertesy, Sakurako Nagumo Wong, Veronica Krenn, Julie Lévi-Strauss, and Juergen A. Knoblich. “Neurotransmitter Signaling Regulates Distinct Phases of Multimodal Human Interneuron Migration.” <i>EMBO Journal</i>. Embo Press, 2021. <a href=\"https://doi.org/10.15252/embj.2021108714\">https://doi.org/10.15252/embj.2021108714</a>.","short":"S. Bajaj, J.A. Bagley, C.M. Sommer, A. Vertesy, S. Nagumo Wong, V. Krenn, J. Lévi-Strauss, J.A. Knoblich, EMBO Journal 40 (2021).","ama":"Bajaj S, Bagley JA, Sommer CM, et al. Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. <i>EMBO Journal</i>. 2021;40(23). doi:<a href=\"https://doi.org/10.15252/embj.2021108714\">10.15252/embj.2021108714</a>","mla":"Bajaj, Sunanjay, et al. “Neurotransmitter Signaling Regulates Distinct Phases of Multimodal Human Interneuron Migration.” <i>EMBO Journal</i>, vol. 40, no. 23, e108714, Embo Press, 2021, doi:<a href=\"https://doi.org/10.15252/embj.2021108714\">10.15252/embj.2021108714</a>.","ista":"Bajaj S, Bagley JA, Sommer CM, Vertesy A, Nagumo Wong S, Krenn V, Lévi-Strauss J, Knoblich JA. 2021. Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. EMBO Journal. 40(23), e108714.","apa":"Bajaj, S., Bagley, J. A., Sommer, C. M., Vertesy, A., Nagumo Wong, S., Krenn, V., … Knoblich, J. A. (2021). Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2021108714\">https://doi.org/10.15252/embj.2021108714</a>"},"date_updated":"2023-08-14T08:05:23Z","abstract":[{"text":"Inhibitory GABAergic interneurons migrate over long distances from their extracortical origin into the developing cortex. In humans, this process is uniquely slow and prolonged, and it is unclear whether guidance cues unique to humans govern the various phases of this complex developmental process. Here, we use fused cerebral organoids to identify key roles of neurotransmitter signaling pathways in guiding the migratory behavior of human cortical interneurons. We use scRNAseq to reveal expression of GABA, glutamate, glycine, and serotonin receptors along distinct maturation trajectories across interneuron migration. We develop an image analysis software package, TrackPal, to simultaneously assess 48 parameters for entire migration tracks of individual cells. By chemical screening, we show that different modes of interneuron migration depend on distinct neurotransmitter signaling pathways, linking transcriptional maturation of interneurons with their migratory behavior. Altogether, our study provides a comprehensive quantitative analysis of human interneuron migration and its functional modulation by neurotransmitter signaling.","lang":"eng"}],"type":"journal_article","month":"10","oa_version":"Published Version","volume":40,"file_date_updated":"2021-12-13T14:54:14Z","date_created":"2021-10-24T22:01:34Z","acknowledgement":"We thank all Knoblich laboratory members for continued support and discussions. We thank the IMP/IMBA BioOptics facility, particularly Pawel Pasierbek, Alberto Moreno Cencerrado and Gerald Schmauss, the IMP/IMBA Molecular Biology Service, in particular Robert Heinen, the IMP Bioinformatics facility, in particular Thomas Burkard, the Vienna Biocenter Core Facilities (VBCF) Histopathology facility, in particular Tamara Engelmaier, and the VBCF Next Generation Sequencing Facility, notably Volodymyr Shubchynskyy and Carmen Czepe. We would also like to thank Simon Haendeler for advice on statistical analyses, Jose Guzman for discussions and assistance with slice culture setups, Oliver L. Eichmueller for discussions and assistance with microscopy, and E.H. Gustafson, S. Wolfinger, and D. Reumann for technical assistance regarding generation of cerebral organoids. This project received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie fellowship agreement Nr.707109 awarded to J.A.B. Work in J.A.K.'s laboratory is supported by the Austrian Federal Ministry of Education, Science and Research, the Austrian Academy of Sciences, the City of Vienna, a Research Program of the Austrian Science Fund FWF (SFBF78 Stem Cell, F 7803-B) and a European Research Council (ERC) Advanced Grant under the European 20 Union’s Horizon 2020 program (grant agreement no. 695642).","year":"2021","_id":"10179"},{"oa":1,"publication_status":"published","has_accepted_license":"1","date_published":"2020-09-01T00:00:00Z","ddc":["580"],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"external_id":{"isi":["000548311800001"],"pmid":["32667089"]},"status":"public","intvolume":"        39","citation":{"ieee":"J. C. Montesinos López <i>et al.</i>, “Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage,” <i>The Embo Journal</i>, vol. 39, no. 17. Embo Press, 2020.","chicago":"Montesinos López, Juan C, A Abuzeineh, Aglaja Kopf, Alba Juanes Garcia, Krisztina Ötvös, J Petrášek, Michael K Sixt, and Eva Benková. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>. Embo Press, 2020. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>.","short":"J.C. Montesinos López, A. Abuzeineh, A. Kopf, A. Juanes Garcia, K. Ötvös, J. Petrášek, M.K. Sixt, E. Benková, The Embo Journal 39 (2020).","ama":"Montesinos López JC, Abuzeineh A, Kopf A, et al. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. 2020;39(17). doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>","ista":"Montesinos López JC, Abuzeineh A, Kopf A, Juanes Garcia A, Ötvös K, Petrášek J, Sixt MK, Benková E. 2020. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. The Embo Journal. 39(17), e104238.","mla":"Montesinos López, Juan C., et al. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>, vol. 39, no. 17, e104238, Embo Press, 2020, doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>.","apa":"Montesinos López, J. C., Abuzeineh, A., Kopf, A., Juanes Garcia, A., Ötvös, K., Petrášek, J., … Benková, E. (2020). Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>"},"type":"journal_article","month":"09","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis. Here, we show that the plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton during cell differentiation and counteracts microtubular rearrangements driven by the hormone auxin. The endogenous upward gradient of cytokinin activity along the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust rearrangements of the microtubule cytoskeleton in epidermal cells progressing from the proliferative to the differentiation stage. Controlled increases in cytokinin activity result in premature re‐organization of the microtubule network from transversal to an oblique disposition in cells prior to their differentiation, whereas attenuated hormone perception delays cytoskeleton conversion into a configuration typical for differentiated cells. Intriguingly, cytokinin can interfere with microtubules also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive control pathway for the microtubular cytoskeleton may be at least partially conserved between plant and animal cells."}],"date_updated":"2023-09-05T13:05:47Z","volume":39,"date_created":"2020-07-21T09:08:38Z","file_date_updated":"2020-12-02T09:13:23Z","acknowledgement":"We thank Takashi Aoyama, David Alabadi, and Bert De Rybel for sharing material, Jiří Friml, Maciek Adamowski, and Katerina Schwarzerová for inspiring discussions, and Martine De Cock for help in preparing the manuscript. This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by the Bioimaging Facility (BIF), especially to Robert Hauschild; and the Life Science Facility (LSF). J.C.M. is the recipient of a EMBO Long‐Term Fellowship (ALTF number 710‐2016). This work was supported with MEYS CR, project no.CZ.02.1.01/0.0/0.0/16_019/0000738 to J.P., and by the Austrian Science Fund (FWF01_I1774S) to E.B.","year":"2020","_id":"8142","publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]},"doi":"10.15252/embj.2019104238","quality_controlled":"1","project":[{"_id":"253E54C8-B435-11E9-9278-68D0E5697425","name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants","grant_number":"ALTF710-2016"},{"name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF","_id":"2542D156-B435-11E9-9278-68D0E5697425","grant_number":"I 1774-B16"}],"isi":1,"issue":"17","language":[{"iso":"eng"}],"author":[{"first_name":"Juan C","last_name":"Montesinos López","full_name":"Montesinos López, Juan C","orcid":"0000-0001-9179-6099","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Abuzeineh","first_name":"A","full_name":"Abuzeineh, A"},{"orcid":"0000-0002-2187-6656","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","full_name":"Kopf, Aglaja","first_name":"Aglaja","last_name":"Kopf"},{"first_name":"Alba","last_name":"Juanes Garcia","id":"40F05888-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1009-9652","full_name":"Juanes Garcia, Alba"},{"orcid":"0000-0002-5503-4983","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","full_name":"Ötvös, Krisztina","first_name":"Krisztina","last_name":"Ötvös"},{"full_name":"Petrášek, J","last_name":"Petrášek","first_name":"J"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","last_name":"Sixt","first_name":"Michael K"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková","first_name":"Eva"}],"day":"01","file":[{"success":1,"file_name":"2020_EMBO_Montesinos.pdf","relation":"main_file","content_type":"application/pdf","file_size":3497156,"creator":"dernst","date_updated":"2020-12-02T09:13:23Z","file_id":"8827","checksum":"43d2b36598708e6ab05c69074e191d57","date_created":"2020-12-02T09:13:23Z","access_level":"open_access"}],"article_number":"e104238","title":"Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Embo Press","department":[{"_id":"MiSi"},{"_id":"EvBe"}],"pmid":1,"publication":"The Embo Journal","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_processing_charge":"Yes (via OA deal)","scopus_import":"1"},{"_id":"6980","year":"2019","volume":38,"date_created":"2019-11-04T15:24:29Z","file_date_updated":"2020-07-14T12:47:46Z","abstract":[{"lang":"eng","text":"Tissue morphogenesis in multicellular organisms is brought about by spatiotemporal coordination of mechanical and chemical signals. Extensive work on how mechanical forces together with the well‐established morphogen signalling pathways can actively shape living tissues has revealed evolutionary conserved mechanochemical features of embryonic development. More recently, attention has been drawn to the description of tissue material properties and how they can influence certain morphogenetic processes. Interestingly, besides the role of tissue material properties in determining how much tissues deform in response to force application, there is increasing theoretical and experimental evidence, suggesting that tissue material properties can abruptly and drastically change in development. These changes resemble phase transitions, pointing at the intriguing possibility that important morphogenetic processes in development, such as symmetry breaking and self‐organization, might be mediated by tissue phase transitions. In this review, we summarize recent findings on the regulation and role of tissue material properties in the context of the developing embryo. We posit that abrupt changes of tissue rheological properties may have important implications in maintaining the balance between robustness and adaptability during embryonic development."}],"date_updated":"2023-09-05T13:04:13Z","oa_version":"Published Version","type":"journal_article","month":"10","intvolume":"        38","citation":{"chicago":"Petridou, Nicoletta, and Carl-Philipp J Heisenberg. “Tissue Rheology in Embryonic Organization.” <i>The EMBO Journal</i>. EMBO, 2019. <a href=\"https://doi.org/10.15252/embj.2019102497\">https://doi.org/10.15252/embj.2019102497</a>.","ieee":"N. Petridou and C.-P. J. Heisenberg, “Tissue rheology in embryonic organization,” <i>The EMBO Journal</i>, vol. 38, no. 20. EMBO, 2019.","short":"N. Petridou, C.-P.J. Heisenberg, The EMBO Journal 38 (2019).","ama":"Petridou N, Heisenberg C-PJ. Tissue rheology in embryonic organization. <i>The EMBO Journal</i>. 2019;38(20). doi:<a href=\"https://doi.org/10.15252/embj.2019102497\">10.15252/embj.2019102497</a>","apa":"Petridou, N., &#38; Heisenberg, C.-P. J. (2019). Tissue rheology in embryonic organization. <i>The EMBO Journal</i>. EMBO. <a href=\"https://doi.org/10.15252/embj.2019102497\">https://doi.org/10.15252/embj.2019102497</a>","mla":"Petridou, Nicoletta, and Carl-Philipp J. Heisenberg. “Tissue Rheology in Embryonic Organization.” <i>The EMBO Journal</i>, vol. 38, no. 20, e102497, EMBO, 2019, doi:<a href=\"https://doi.org/10.15252/embj.2019102497\">10.15252/embj.2019102497</a>.","ista":"Petridou N, Heisenberg C-PJ. 2019. Tissue rheology in embryonic organization. The EMBO Journal. 38(20), e102497."},"external_id":{"pmid":["31512749"],"isi":["000485561900001"]},"status":"public","date_published":"2019-10-15T00:00:00Z","ddc":["570"],"oa":1,"publication_status":"published","has_accepted_license":"1","publication":"The EMBO Journal","article_processing_charge":"Yes (via OA deal)","ec_funded":1,"scopus_import":"1","article_type":"review","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"publisher":"EMBO","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","pmid":1,"department":[{"_id":"CaHe"}],"title":"Tissue rheology in embryonic organization","article_number":"e102497","author":[{"last_name":"Petridou","first_name":"Nicoletta","full_name":"Petridou, Nicoletta","orcid":"0000-0002-8451-1195","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"day":"15","file":[{"access_level":"open_access","date_created":"2019-11-04T15:30:08Z","checksum":"76f7f4e79ab6d850c30017a69726fd85","date_updated":"2020-07-14T12:47:46Z","file_id":"6981","creator":"dernst","relation":"main_file","content_type":"application/pdf","file_size":847356,"file_name":"2019_Embo_Petridou.pdf"}],"isi":1,"language":[{"iso":"eng"}],"issue":"20","project":[{"call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573"},{"grant_number":"V00736","_id":"2693FD8C-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Tissue material properties in embryonic development"}],"doi":"10.15252/embj.2019102497","quality_controlled":"1","publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]}},{"date_published":"2018-08-01T00:00:00Z","ddc":["570"],"oa":1,"publication_status":"published","has_accepted_license":"1","intvolume":"        37","citation":{"short":"S.M. Truckenbrodt, A. Viplav, S. Jähne, A. Vogts, A. Denker, H. Wildhagen, E. Fornasiero, S. Rizzoli, The EMBO Journal 37 (2018).","ieee":"S. M. Truckenbrodt <i>et al.</i>, “Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission,” <i>The EMBO Journal</i>, vol. 37, no. 15. Wiley, 2018.","chicago":"Truckenbrodt, Sven M, Abhiyan Viplav, Sebsatian Jähne, Angela Vogts, Annette Denker, Hanna Wildhagen, Eugenio Fornasiero, and Silvio Rizzoli. “Newly Produced Synaptic Vesicle Proteins Are Preferentially Used in Synaptic Transmission.” <i>The EMBO Journal</i>. Wiley, 2018. <a href=\"https://doi.org/10.15252/embj.201798044\">https://doi.org/10.15252/embj.201798044</a>.","mla":"Truckenbrodt, Sven M., et al. “Newly Produced Synaptic Vesicle Proteins Are Preferentially Used in Synaptic Transmission.” <i>The EMBO Journal</i>, vol. 37, no. 15, e98044, Wiley, 2018, doi:<a href=\"https://doi.org/10.15252/embj.201798044\">10.15252/embj.201798044</a>.","ista":"Truckenbrodt SM, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero E, Rizzoli S. 2018. Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission. The EMBO Journal. 37(15), e98044.","apa":"Truckenbrodt, S. M., Viplav, A., Jähne, S., Vogts, A., Denker, A., Wildhagen, H., … Rizzoli, S. (2018). Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission. <i>The EMBO Journal</i>. Wiley. <a href=\"https://doi.org/10.15252/embj.201798044\">https://doi.org/10.15252/embj.201798044</a>","ama":"Truckenbrodt SM, Viplav A, Jähne S, et al. Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission. <i>The EMBO Journal</i>. 2018;37(15). doi:<a href=\"https://doi.org/10.15252/embj.201798044\">10.15252/embj.201798044</a>"},"status":"public","external_id":{"isi":["000440416900005"],"pmid":["29950309"]},"volume":37,"file_date_updated":"2020-07-14T12:44:56Z","date_created":"2018-12-11T11:44:52Z","month":"08","oa_version":"Published Version","type":"journal_article","date_updated":"2023-09-13T09:02:48Z","abstract":[{"lang":"eng","text":"Aged proteins can become hazardous to cellular function, by accumulating molecular damage. This implies that cells should preferentially rely on newly produced ones. We tested this hypothesis in cultured hippocampal neurons, focusing on synaptic transmission. We found that newly synthesized vesicle proteins were incorporated in the actively recycling pool of vesicles responsible for all neurotransmitter release during physiological activity. We observed this for the calcium sensor Synaptotagmin 1, for the neurotransmitter transporter VGAT, and for the fusion protein VAMP2 (Synaptobrevin 2). Metabolic labeling of proteins and visualization by secondary ion mass spectrometry enabled us to query the entire protein makeup of the actively recycling vesicles, which we found to be younger than that of non-recycling vesicles. The young vesicle proteins remained in use for up to ~ 24 h, during which they participated in recycling a few hundred times. They were afterward reluctant to release and were degraded after an additional ~ 24–48 h. We suggest that the recycling pool of synaptic vesicles relies on newly synthesized proteins, while the inactive reserve pool contains older proteins."}],"_id":"145","acknowledgement":"We thank Reinhard Jahn for providing a plasmid for YFP-SNAP25. We thank Erwin Neher for help with the development of the mathematical model of the synaptic vesicle life cycle. We thank Martin Meschkat, Andreas Höbartner, Annedore Punge, and Peer Hoopmann for help with the experiments. We thank Burkhard Rammner for providing the illustrations of synaptic vesicle and protein dynamics. We thank Manuel Maidorn, Martin Helm, and Katharina N. Richter for critically reading the manuscript. S.T. was supported by an Excellence Stipend of the Göttingen Graduate School for Neurosciences, Biophysics, and Molecular Biosciences (GGNB). E.F.F. is a recipient of long-term fellowships from the European Molecular Biology Organization (ALTF_797-2012) and from the Human Frontier Science Program (HFSP_LT000830/2013). The work was supported by grants to S.O.R. from the European Research Council (ERC-2013-CoG NeuroMolAnatomy) and from the Deutsche Forschungsgemeinschaft (Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain, SFB1190/P09, SFB889/A05, and SFB1286/A03, and DFG RI 1967 7/1). The nanoSIMS instrument was funded by the German Federal Ministry of Education and Research (03F0626A).","year":"2018","doi":"10.15252/embj.201798044","quality_controlled":"1","publication_identifier":{"issn":["0261-4189"]},"isi":1,"issue":"15","language":[{"iso":"eng"}],"article_number":"e98044","title":"Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission","publist_id":"7778","author":[{"id":"45812BD4-F248-11E8-B48F-1D18A9856A87","full_name":"Truckenbrodt, Sven M","first_name":"Sven M","last_name":"Truckenbrodt"},{"first_name":"Abhiyan","last_name":"Viplav","full_name":"Viplav, Abhiyan"},{"first_name":"Sebsatian","last_name":"Jähne","full_name":"Jähne, Sebsatian"},{"full_name":"Vogts, Angela","last_name":"Vogts","first_name":"Angela"},{"full_name":"Denker, Annette","first_name":"Annette","last_name":"Denker"},{"last_name":"Wildhagen","first_name":"Hanna","full_name":"Wildhagen, Hanna"},{"last_name":"Fornasiero","first_name":"Eugenio","full_name":"Fornasiero, Eugenio"},{"full_name":"Rizzoli, Silvio","last_name":"Rizzoli","first_name":"Silvio"}],"file":[{"file_name":"2018_EMBO_Truckenbrodt.pdf","creator":"dernst","relation":"main_file","content_type":"application/pdf","file_size":2846470,"checksum":"a540feb6c9af6aefc78de531461a8835","date_updated":"2020-07-14T12:44:56Z","file_id":"5710","access_level":"open_access","date_created":"2018-12-17T14:17:29Z"}],"day":"01","publication":"The EMBO Journal","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_processing_charge":"No","scopus_import":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Wiley","department":[{"_id":"JoDa"}],"pmid":1},{"status":"public","external_id":{"pmid":["11387211"]},"extern":"1","intvolume":"        20","citation":{"short":"A. Molendijk, F. Bischoff, C. Rajendrakumar, J. Friml, M. Braun, S. Gilroy, K. Palme, EMBO Journal 20 (2001) 2779–2788.","ieee":"A. Molendijk <i>et al.</i>, “Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth,” <i>EMBO Journal</i>, vol. 20, no. 11. Wiley-Blackwell, pp. 2779–2788, 2001.","chicago":"Molendijk, Arthur, Friedrich Bischoff, Chadalavada Rajendrakumar, Jiří Friml, Markus Braun, Simon Gilroy, and Klaus Palme. “Arabidopsis Thaliana Rop GTPases Are Localized to Tips of Root Hairs and Control Polar Growth.” <i>EMBO Journal</i>. Wiley-Blackwell, 2001. <a href=\"https://doi.org/10.1093/emboj/20.11.2779\">https://doi.org/10.1093/emboj/20.11.2779</a>.","mla":"Molendijk, Arthur, et al. “Arabidopsis Thaliana Rop GTPases Are Localized to Tips of Root Hairs and Control Polar Growth.” <i>EMBO Journal</i>, vol. 20, no. 11, Wiley-Blackwell, 2001, pp. 2779–88, doi:<a href=\"https://doi.org/10.1093/emboj/20.11.2779\">10.1093/emboj/20.11.2779</a>.","ista":"Molendijk A, Bischoff F, Rajendrakumar C, Friml J, Braun M, Gilroy S, Palme K. 2001. Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. EMBO Journal. 20(11), 2779–2788.","apa":"Molendijk, A., Bischoff, F., Rajendrakumar, C., Friml, J., Braun, M., Gilroy, S., &#38; Palme, K. (2001). Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1093/emboj/20.11.2779\">https://doi.org/10.1093/emboj/20.11.2779</a>","ama":"Molendijk A, Bischoff F, Rajendrakumar C, et al. Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. <i>EMBO Journal</i>. 2001;20(11):2779-2788. doi:<a href=\"https://doi.org/10.1093/emboj/20.11.2779\">10.1093/emboj/20.11.2779</a>"},"oa":1,"publication_status":"published","date_published":"2001-06-01T00:00:00Z","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC125484/","open_access":"1"}],"acknowledgement":"We thank Drs Frantisek Baluška, Matthias Godde, Peter Huijser, Lars Vahlkamp and Dieter Volkmann for help, criticism and constructive reading of the manuscript. We are grateful to Dr N.-H.Chua for providing us with pTA7002. The work was funded by the DFG, the European Communities Biotechnology Programme (Bio4-CT98 0239) and the INCO Copernicus Programme (IC15-CT96-0920). C.S.V.R. is the recipient of an Alexander von Humboldt fellowship and J.F. of a DAAD fellowship.","year":"2001","_id":"2981","page":"2779 - 2788","abstract":[{"lang":"eng","text":"Plants contain a novel unique subfamily of Rho GTPases, vital components of cellular signalling networks. Here we report a general role for some members of this family in polarized plant growth processes. We show that Arabidopsis AtRop4 and AtRop6 encode functional GTPases with similar intrinsic GTP hydrolysis rates. We localized AtRop proteins in root meristem cells to the cross-wall and cell plate membranes. Polar localization of AtRops in trichoblasts specifies the growth sites for emerging root hairs. These sites were visible before budding and elongation of the Arabidopsis root hair when AtRops accumulated at their tips. Expression of constitutively active AtRop4 and AtRop6 mutant proteins in root hairs of transgenic Arabidopsis plants abolished polarized growth and delocalized the tip-focused Ca2+ gradient. Polar localization of AtRops was inhibited by brefeldin A, but not by other drugs such as latrunculin B, cytochalasin D or caffeine. Our results demonstrate a general function of AtRop GTPases in tip growth and in polar diffuse growth."}],"date_updated":"2023-05-16T12:07:45Z","oa_version":"Published Version","type":"journal_article","month":"06","volume":20,"date_created":"2018-12-11T12:00:40Z","language":[{"iso":"eng"}],"issue":"11","publication_identifier":{"issn":["0261-4189"]},"doi":"10.1093/emboj/20.11.2779","quality_controlled":"1","publisher":"Wiley-Blackwell","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","pmid":1,"publication":"EMBO Journal","scopus_import":"1","article_processing_charge":"No","article_type":"original","author":[{"last_name":"Molendijk","first_name":"Arthur","full_name":"Molendijk, Arthur"},{"last_name":"Bischoff","first_name":"Friedrich","full_name":"Bischoff, Friedrich"},{"last_name":"Rajendrakumar","first_name":"Chadalavada","full_name":"Rajendrakumar, Chadalavada"},{"full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml"},{"last_name":"Braun","first_name":"Markus","full_name":"Braun, Markus"},{"full_name":"Gilroy, Simon","last_name":"Gilroy","first_name":"Simon"},{"full_name":"Palme, Klaus","last_name":"Palme","first_name":"Klaus"}],"day":"01","title":"Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth","publist_id":"3721"},{"volume":17,"date_created":"2018-12-11T11:54:54Z","page":"868 - 876","date_updated":"2022-09-01T13:17:49Z","abstract":[{"text":"The plastid genomes of several plants contain homologues, termed ndh genes, of genes encoding subunits of the NADH:ubiquinone oxidoreductase or complex I of mitochondria and eubacteria. The functional significance of the Ndh proteins in higher plants is uncertain. We show here that tobacco chloroplasts contain a protein complex of 550 kDa consisting of at least three of the ndh gene products: NdhI, NdhJ and NdhK. We have constructed mutant tobacco plants with disrupted ndhC, ndhK and ndhJ plastid genes, indicating that the Ndh complex is dispensible for plant growth under optimal growth conditions. Chlorophyll fluorescence analysis shows that in vivo the Ndh complex catalyses the post-illumination reduction of the plastoquinone pool and in the light optimizes the induction of photosynthesis under conditions of water stress. We conclude that the Ndh complex catalyses the reduction of the plastoquinone pool using stromal reductant and so acts as a respiratory complex. Overall, our data are compatible with the participation of the Ndh complex in cyclic electron flow around the photosystem I complex in the light and possibly in a chloroplast respiratory chain in the dark.","lang":"eng"}],"month":"02","type":"journal_article","oa_version":"None","_id":"1955","acknowledgement":"We thank Professor Süss (Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany) for the gift of the anti-FNR antiserum, Professor Masahiro Sugiura (Nagoya University, Japan) for the gift of plasmid pTB19 and Professor Peter Horton (University of Sheffield) for the loan of his ED-800T unit. P.B. is a recipient of a BBSRC studentship and the work was supported by grants from the BBSRC, The Royal Society (to P.J.N.) and The National Science Foundation (to P.M.).","year":"1998","date_published":"1998-02-04T00:00:00Z","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170436/","open_access":"1"}],"oa":1,"publication_status":"published","extern":"1","intvolume":"        17","citation":{"short":"P. Burrows, L.A. Sazanov, Z. Sváb, P. Maliga, P. Nixon, EMBO Journal 17 (1998) 868–876.","ieee":"P. Burrows, L. A. Sazanov, Z. Sváb, P. Maliga, and P. Nixon, “Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes,” <i>EMBO Journal</i>, vol. 17, no. 4. Wiley-Blackwell, pp. 868–876, 1998.","chicago":"Burrows, Paul, Leonid A Sazanov, Zóra Sváb, Pàl Maliga, and Peter Nixon. “Identification of a Functional Respiratory Complex in Chloroplasts through Analysis of Tobacco Mutants Containing Disrupted Plastid Ndh Genes.” <i>EMBO Journal</i>. Wiley-Blackwell, 1998. <a href=\"https://doi.org/10.1093/emboj/17.4.868\">https://doi.org/10.1093/emboj/17.4.868</a>.","mla":"Burrows, Paul, et al. “Identification of a Functional Respiratory Complex in Chloroplasts through Analysis of Tobacco Mutants Containing Disrupted Plastid Ndh Genes.” <i>EMBO Journal</i>, vol. 17, no. 4, Wiley-Blackwell, 1998, pp. 868–76, doi:<a href=\"https://doi.org/10.1093/emboj/17.4.868\">10.1093/emboj/17.4.868</a>.","ista":"Burrows P, Sazanov LA, Sváb Z, Maliga P, Nixon P. 1998. Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes. EMBO Journal. 17(4), 868–876.","apa":"Burrows, P., Sazanov, L. A., Sváb, Z., Maliga, P., &#38; Nixon, P. (1998). Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1093/emboj/17.4.868\">https://doi.org/10.1093/emboj/17.4.868</a>","ama":"Burrows P, Sazanov LA, Sváb Z, Maliga P, Nixon P. Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes. <i>EMBO Journal</i>. 1998;17(4):868-876. doi:<a href=\"https://doi.org/10.1093/emboj/17.4.868\">10.1093/emboj/17.4.868</a>"},"external_id":{"pmid":["9463365"]},"status":"public","title":"Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes","publist_id":"5129","author":[{"full_name":"Burrows, Paul","first_name":"Paul","last_name":"Burrows"},{"full_name":"Sazanov, Leonid A","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0977-7989","last_name":"Sazanov","first_name":"Leonid A"},{"full_name":"Sváb, Zóra","last_name":"Sváb","first_name":"Zóra"},{"full_name":"Maliga, Pàl","first_name":"Pàl","last_name":"Maliga"},{"full_name":"Nixon, Peter","first_name":"Peter","last_name":"Nixon"}],"day":"04","publication":"EMBO Journal","article_processing_charge":"No","scopus_import":"1","article_type":"original","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"Wiley-Blackwell","pmid":1,"doi":"10.1093/emboj/17.4.868","quality_controlled":"1","publication_identifier":{"issn":["0261-4189"]},"language":[{"iso":"eng"}],"issue":"4"}]