---
_id: '14834'
abstract:
- lang: eng
  text: Bacteria divide by binary fission. The protein machine responsible for this
    process is the divisome, a transient assembly of more than 30 proteins in and
    on the surface of the cytoplasmic membrane. Together, they constrict the cell
    envelope and remodel the peptidoglycan layer to eventually split the cell into
    two. For Escherichia coli, most molecular players involved in this process have
    probably been identified, but obtaining the quantitative information needed for
    a mechanistic understanding can often not be achieved from experiments in vivo
    alone. Since the discovery of the Z-ring more than 30 years ago, in vitro reconstitution
    experiments have been crucial to shed light on molecular processes normally hidden
    in the complex environment of the living cell. In this review, we summarize how
    rebuilding the divisome from purified components – or at least parts of it - have
    been instrumental to obtain the detailed mechanistic understanding of the bacterial
    cell division machinery that we have today.
acknowledgement: We acknowledge members of the Loose laboratory at ISTA for helpful
  discussions—in particular M. Kojic for his insightful comments. This work was supported
  by the Austrian Science Fund (FWF P34607) to M.L.
article_number: '151380'
article_processing_charge: Yes
article_type: review
author:
- first_name: Philipp
  full_name: Radler, Philipp
  id: 40136C2A-F248-11E8-B48F-1D18A9856A87
  last_name: Radler
  orcid: '0000-0001-9198-2182 '
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
citation:
  ama: 'Radler P, Loose M. A dynamic duo: Understanding the roles of FtsZ and FtsA
    for Escherichia coli cell division through in vitro approaches. <i>European Journal
    of Cell Biology</i>. 2024;103(1). doi:<a href="https://doi.org/10.1016/j.ejcb.2023.151380">10.1016/j.ejcb.2023.151380</a>'
  apa: 'Radler, P., &#38; Loose, M. (2024). A dynamic duo: Understanding the roles
    of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches.
    <i>European Journal of Cell Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.ejcb.2023.151380">https://doi.org/10.1016/j.ejcb.2023.151380</a>'
  chicago: 'Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles
    of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.”
    <i>European Journal of Cell Biology</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.ejcb.2023.151380">https://doi.org/10.1016/j.ejcb.2023.151380</a>.'
  ieee: 'P. Radler and M. Loose, “A dynamic duo: Understanding the roles of FtsZ and
    FtsA for Escherichia coli cell division through in vitro approaches,” <i>European
    Journal of Cell Biology</i>, vol. 103, no. 1. Elsevier, 2024.'
  ista: 'Radler P, Loose M. 2024. A dynamic duo: Understanding the roles of FtsZ and
    FtsA for Escherichia coli cell division through in vitro approaches. European
    Journal of Cell Biology. 103(1), 151380.'
  mla: 'Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles
    of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.”
    <i>European Journal of Cell Biology</i>, vol. 103, no. 1, 151380, Elsevier, 2024,
    doi:<a href="https://doi.org/10.1016/j.ejcb.2023.151380">10.1016/j.ejcb.2023.151380</a>.'
  short: P. Radler, M. Loose, European Journal of Cell Biology 103 (2024).
date_created: 2024-01-18T08:16:43Z
date_published: 2024-01-12T00:00:00Z
date_updated: 2024-01-23T08:37:13Z
day: '12'
ddc:
- '570'
department:
- _id: MaLo
doi: 10.1016/j.ejcb.2023.151380
external_id:
  pmid:
  - '38218128'
has_accepted_license: '1'
intvolume: '       103'
issue: '1'
keyword:
- Cell Biology
- General Medicine
- Histology
- Pathology and Forensic Medicine
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.ejcb.2023.151380
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: fc38323b-9c52-11eb-aca3-ff8afb4a011d
  grant_number: P34607
  name: "Understanding bacterial cell division by in vitro\r\nreconstitution"
publication: European Journal of Cell Biology
publication_identifier:
  issn:
  - 0171-9335
publication_status: epub_ahead
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli
  cell division through in vitro approaches'
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 103
year: '2024'
...
---
_id: '2577'
abstract:
- lang: eng
  text: The cloned cDNA for rat prostacyclin synthase was found to contain a 1503-bp
    open reading frame which encoded a 501-amino acid protein sharing 84% identity
    with the human enzyme. RNA blot analysis revealed that the rat prostacyclin synthase
    mRNA, as a single species of 2.1 kb, is expressed abundantly in the aorta and
    uterus. High levels of expression were also observed in the stomach, lung, heart,
    testis, liver, and skeletal muscle. Low but significant expression was also seen
    in the brain and kidney. Furthermore, the regional distribution and cellular localization
    of prostacyclin synthase mRNA were examined by in situ hybridization analysis
    of rat tissue sections. The definitive signals for the mRNA were localized in
    smooth muscle cells of the arteries, bronchi and uterus, and in the cells of the
    fibrous tunic surrounding the seminiferous tubules, which are characterized as
    smooth muscle cells. Besides smooth muscle cells, signal were also detected in
    the fibroblasts of the heart myocardium, lung parenchyma cells and kidney inner
    medulla tubules and interstitial cells.
article_processing_charge: No
article_type: original
author:
- first_name: Yoshinori
  full_name: Tone, Yoshinori
  last_name: Tone
- first_name: Hiroyasu
  full_name: Inoue, Hiroyasu
  last_name: Inoue
- first_name: Shuntaro
  full_name: Hara, Shuntaro
  last_name: Hara
- first_name: Chieko
  full_name: Yokoyama, Chieko
  last_name: Yokoyama
- first_name: Toshihisa
  full_name: Hatae, Toshihisa
  last_name: Hatae
- first_name: Hiroji
  full_name: Oida, Hiroji
  last_name: Oida
- first_name: Shuh
  full_name: Narumiya, Shuh
  last_name: Narumiya
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Susumu
  full_name: Yukawa, Susumu
  last_name: Yukawa
- first_name: Tadashi
  full_name: Tanabe, Tadashi
  last_name: Tanabe
citation:
  ama: Tone Y, Inoue H, Hara S, et al. The regional distribution and cellular localization
    of mRNA encoding rat prostacyclin synthase. <i>European Journal of Cell Biology</i>.
    1997;72(3):268-277.
  apa: Tone, Y., Inoue, H., Hara, S., Yokoyama, C., Hatae, T., Oida, H., … Tanabe,
    T. (1997). The regional distribution and cellular localization of mRNA encoding
    rat prostacyclin synthase. <i>European Journal of Cell Biology</i>. Elsevier.
  chicago: Tone, Yoshinori, Hiroyasu Inoue, Shuntaro Hara, Chieko Yokoyama, Toshihisa
    Hatae, Hiroji Oida, Shuh Narumiya, Ryuichi Shigemoto, Susumu Yukawa, and Tadashi
    Tanabe. “The Regional Distribution and Cellular Localization of MRNA Encoding
    Rat Prostacyclin Synthase.” <i>European Journal of Cell Biology</i>. Elsevier,
    1997.
  ieee: Y. Tone <i>et al.</i>, “The regional distribution and cellular localization
    of mRNA encoding rat prostacyclin synthase,” <i>European Journal of Cell Biology</i>,
    vol. 72, no. 3. Elsevier, pp. 268–277, 1997.
  ista: Tone Y, Inoue H, Hara S, Yokoyama C, Hatae T, Oida H, Narumiya S, Shigemoto
    R, Yukawa S, Tanabe T. 1997. The regional distribution and cellular localization
    of mRNA encoding rat prostacyclin synthase. European Journal of Cell Biology.
    72(3), 268–277.
  mla: Tone, Yoshinori, et al. “The Regional Distribution and Cellular Localization
    of MRNA Encoding Rat Prostacyclin Synthase.” <i>European Journal of Cell Biology</i>,
    vol. 72, no. 3, Elsevier, 1997, pp. 268–77.
  short: Y. Tone, H. Inoue, S. Hara, C. Yokoyama, T. Hatae, H. Oida, S. Narumiya,
    R. Shigemoto, S. Yukawa, T. Tanabe, European Journal of Cell Biology 72 (1997)
    268–277.
date_created: 2018-12-11T11:58:29Z
date_published: 1997-03-01T00:00:00Z
date_updated: 2022-08-22T12:50:04Z
day: '01'
extern: '1'
external_id:
  pmid:
  - '9084989 '
intvolume: '        72'
issue: '3'
language:
- iso: eng
month: '03'
oa_version: None
page: 268 - 277
pmid: 1
publication: European Journal of Cell Biology
publication_identifier:
  issn:
  - 0171-9335
publication_status: published
publisher: Elsevier
publist_id: '4321'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The regional distribution and cellular localization of mRNA encoding rat prostacyclin
  synthase
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 72
year: '1997'
...