[{"oa":1,"volume":103,"date_updated":"2024-01-23T08:37:13Z","article_processing_charge":"Yes","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"We acknowledge members of the Loose laboratory at ISTA for helpful discussions—in particular M. Kojic for his insightful comments. This work was supported by the Austrian Science Fund (FWF P34607) to M.L.","quality_controlled":"1","project":[{"_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d","name":"Understanding bacterial cell division by in vitro\r\nreconstitution","grant_number":"P34607"}],"oa_version":"Published Version","pmid":1,"_id":"14834","publication_identifier":{"issn":["0171-9335"]},"publication_status":"epub_ahead","citation":{"chicago":"Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.” <i>European Journal of Cell Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">https://doi.org/10.1016/j.ejcb.2023.151380</a>.","apa":"Radler, P., &#38; Loose, M. (2024). A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches. <i>European Journal of Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">https://doi.org/10.1016/j.ejcb.2023.151380</a>","ieee":"P. Radler and M. Loose, “A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches,” <i>European Journal of Cell Biology</i>, vol. 103, no. 1. Elsevier, 2024.","ista":"Radler P, Loose M. 2024. A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches. European Journal of Cell Biology. 103(1), 151380.","short":"P. Radler, M. Loose, European Journal of Cell Biology 103 (2024).","mla":"Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.” <i>European Journal of Cell Biology</i>, vol. 103, no. 1, 151380, Elsevier, 2024, doi:<a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">10.1016/j.ejcb.2023.151380</a>.","ama":"Radler P, Loose M. A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches. <i>European Journal of Cell Biology</i>. 2024;103(1). doi:<a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">10.1016/j.ejcb.2023.151380</a>"},"author":[{"id":"40136C2A-F248-11E8-B48F-1D18A9856A87","first_name":"Philipp","full_name":"Radler, Philipp","last_name":"Radler","orcid":"0000-0001-9198-2182 "},{"id":"462D4284-F248-11E8-B48F-1D18A9856A87","last_name":"Loose","full_name":"Loose, Martin","orcid":"0000-0001-7309-9724","first_name":"Martin"}],"keyword":["Cell Biology","General Medicine","Histology","Pathology and Forensic Medicine"],"abstract":[{"text":"Bacteria divide by binary fission. The protein machine responsible for this process is the divisome, a transient assembly of more than 30 proteins in and on the surface of the cytoplasmic membrane. Together, they constrict the cell envelope and remodel the peptidoglycan layer to eventually split the cell into two. For Escherichia coli, most molecular players involved in this process have probably been identified, but obtaining the quantitative information needed for a mechanistic understanding can often not be achieved from experiments in vivo alone. Since the discovery of the Z-ring more than 30 years ago, in vitro reconstitution experiments have been crucial to shed light on molecular processes normally hidden in the complex environment of the living cell. In this review, we summarize how rebuilding the divisome from purified components – or at least parts of it - have been instrumental to obtain the detailed mechanistic understanding of the bacterial cell division machinery that we have today.","lang":"eng"}],"article_number":"151380","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.ejcb.2023.151380"}],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"doi":"10.1016/j.ejcb.2023.151380","year":"2024","external_id":{"pmid":["38218128"]},"title":"A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches","issue":"1","publication":"European Journal of Cell Biology","type":"journal_article","day":"12","status":"public","intvolume":"       103","department":[{"_id":"MaLo"}],"has_accepted_license":"1","date_created":"2024-01-18T08:16:43Z","article_type":"review","date_published":"2024-01-12T00:00:00Z","month":"01","language":[{"iso":"eng"}],"publisher":"Elsevier","scopus_import":"1"},{"citation":{"ista":"Tone Y, Inoue H, Hara S, Yokoyama C, Hatae T, Oida H, Narumiya S, Shigemoto R, Yukawa S, Tanabe T. 1997. The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase. European Journal of Cell Biology. 72(3), 268–277.","short":"Y. Tone, H. Inoue, S. Hara, C. Yokoyama, T. Hatae, H. Oida, S. Narumiya, R. Shigemoto, S. Yukawa, T. Tanabe, European Journal of Cell Biology 72 (1997) 268–277.","ama":"Tone Y, Inoue H, Hara S, et al. The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase. <i>European Journal of Cell Biology</i>. 1997;72(3):268-277.","mla":"Tone, Yoshinori, et al. “The Regional Distribution and Cellular Localization of MRNA Encoding Rat Prostacyclin Synthase.” <i>European Journal of Cell Biology</i>, vol. 72, no. 3, Elsevier, 1997, pp. 268–77.","chicago":"Tone, Yoshinori, Hiroyasu Inoue, Shuntaro Hara, Chieko Yokoyama, Toshihisa Hatae, Hiroji Oida, Shuh Narumiya, Ryuichi Shigemoto, Susumu Yukawa, and Tadashi Tanabe. “The Regional Distribution and Cellular Localization of MRNA Encoding Rat Prostacyclin Synthase.” <i>European Journal of Cell Biology</i>. Elsevier, 1997.","ieee":"Y. Tone <i>et al.</i>, “The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase,” <i>European Journal of Cell Biology</i>, vol. 72, no. 3. Elsevier, pp. 268–277, 1997.","apa":"Tone, Y., Inoue, H., Hara, S., Yokoyama, C., Hatae, T., Oida, H., … Tanabe, T. (1997). The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase. <i>European Journal of Cell Biology</i>. Elsevier."},"publication_status":"published","author":[{"full_name":"Tone, Yoshinori","last_name":"Tone","first_name":"Yoshinori"},{"first_name":"Hiroyasu","full_name":"Inoue, Hiroyasu","last_name":"Inoue"},{"first_name":"Shuntaro","full_name":"Hara, Shuntaro","last_name":"Hara"},{"first_name":"Chieko","last_name":"Yokoyama","full_name":"Yokoyama, Chieko"},{"first_name":"Toshihisa","full_name":"Hatae, Toshihisa","last_name":"Hatae"},{"first_name":"Hiroji","last_name":"Oida","full_name":"Oida, Hiroji"},{"first_name":"Shuh","full_name":"Narumiya, Shuh","last_name":"Narumiya"},{"id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi","last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444"},{"first_name":"Susumu","last_name":"Yukawa","full_name":"Yukawa, Susumu"},{"full_name":"Tanabe, Tadashi","last_name":"Tanabe","first_name":"Tadashi"}],"abstract":[{"text":"The cloned cDNA for rat prostacyclin synthase was found to contain a 1503-bp open reading frame which encoded a 501-amino acid protein sharing 84% identity with the human enzyme. RNA blot analysis revealed that the rat prostacyclin synthase mRNA, as a single species of 2.1 kb, is expressed abundantly in the aorta and uterus. High levels of expression were also observed in the stomach, lung, heart, testis, liver, and skeletal muscle. Low but significant expression was also seen in the brain and kidney. Furthermore, the regional distribution and cellular localization of prostacyclin synthase mRNA were examined by in situ hybridization analysis of rat tissue sections. The definitive signals for the mRNA were localized in smooth muscle cells of the arteries, bronchi and uterus, and in the cells of the fibrous tunic surrounding the seminiferous tubules, which are characterized as smooth muscle cells. Besides smooth muscle cells, signal were also detected in the fibroblasts of the heart myocardium, lung parenchyma cells and kidney inner medulla tubules and interstitial cells.","lang":"eng"}],"article_processing_charge":"No","publist_id":"4321","date_updated":"2022-08-22T12:50:04Z","volume":72,"quality_controlled":"1","oa_version":"None","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","extern":"1","publication_identifier":{"issn":["0171-9335"]},"_id":"2577","pmid":1,"year":"1997","external_id":{"pmid":["9084989 "]},"title":"The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase","type":"journal_article","day":"01","status":"public","intvolume":"        72","page":"268 - 277","publication":"European Journal of Cell Biology","issue":"3","date_published":"1997-03-01T00:00:00Z","article_type":"original","month":"03","language":[{"iso":"eng"}],"scopus_import":"1","publisher":"Elsevier","date_created":"2018-12-11T11:58:29Z"}]