---
_id: '3495'
abstract:
- lang: eng
  text: High Ca2+ permeability and its control by voltage-dependent Mg2+ block are
    defining features of NMDA receptors. These features are lost if the principal
    NR1 subunit carries an asparagine (N) to arginine (R) substitution in a critical
    channel site at NR1 position 598. NR1(R) expression from a single allele in gene-targeted
    NR1+/R mice is lethal soon after birth, precluding analysis of altered synaptic
    functions later in life. We therefore employed the forebrain specific αCaMKII
    promoter to drive tTA-mediated tetracyclin sensitive transcription of transgenes
    for NR1(R) and for lacZ as reporter. Transgene expression was observed in cortex,
    striatum, hippocampus, amygdala and olfactory bulb and was mosaic in all these
    forebrain regions. It was highest in olfactory bulb granule cells, in most of
    which Ca2+ permeability and voltage-dependent Mg2+ block of NMDA receptors were
    reduced to different extents. This indicates significant impairment of NMDA receptor
    function by NR1(R) in presence of the wild-type NR1 complement. Indeed, even though
    NR1(R) mRNA constituted only 18% of the entire NR1 mRNA population in forebrain,
    the transgenic mice died during adolescence unless transgene expression was suppressed
    by doxycycline. Thus, glutamate receptor function can be altered in the mouse
    by regulated NR1(R) transgene expression.
acknowledgement: 'This work was supported in part by grants from the 358 (1992) 36–41.Deutsche
  Forschungsgemeinschaft (Bi 642 / 1-2) and the Volkswagen foundation. '
article_processing_charge: No
article_type: original
author:
- first_name: Jasna
  full_name: Jerecic, Jasna
  last_name: Jerecic
- first_name: Christian
  full_name: Schulze, Christian
  last_name: Schulze
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
- first_name: Rolf
  full_name: Sprengel, Rolf
  last_name: Sprengel
- first_name: Peter
  full_name: Seeburg, Peter
  last_name: Seeburg
- first_name: Joseph
  full_name: Bischofberger, Joseph
  last_name: Bischofberger
citation:
  ama: Jerecic J, Schulze C, Jonas PM, Sprengel R, Seeburg P, Bischofberger J. Impaired
    NMDA receptor function in mouse olfactory bulb neurons by tetracycline-sensitive
    NR1 (N598R) expression. <i>Molecular Brain Research</i>. 2001;94(1-2):96-104.
    doi:<a href="https://doi.org/10.1016/S0169-328X(01)00221-2">10.1016/S0169-328X(01)00221-2</a>
  apa: Jerecic, J., Schulze, C., Jonas, P. M., Sprengel, R., Seeburg, P., &#38; Bischofberger,
    J. (2001). Impaired NMDA receptor function in mouse olfactory bulb neurons by
    tetracycline-sensitive NR1 (N598R) expression. <i>Molecular Brain Research</i>.
    Elsevier. <a href="https://doi.org/10.1016/S0169-328X(01)00221-2">https://doi.org/10.1016/S0169-328X(01)00221-2</a>
  chicago: Jerecic, Jasna, Christian Schulze, Peter M Jonas, Rolf Sprengel, Peter
    Seeburg, and Joseph Bischofberger. “Impaired NMDA Receptor Function in Mouse Olfactory
    Bulb Neurons by Tetracycline-Sensitive NR1 (N598R) Expression.” <i>Molecular Brain
    Research</i>. Elsevier, 2001. <a href="https://doi.org/10.1016/S0169-328X(01)00221-2">https://doi.org/10.1016/S0169-328X(01)00221-2</a>.
  ieee: J. Jerecic, C. Schulze, P. M. Jonas, R. Sprengel, P. Seeburg, and J. Bischofberger,
    “Impaired NMDA receptor function in mouse olfactory bulb neurons by tetracycline-sensitive
    NR1 (N598R) expression,” <i>Molecular Brain Research</i>, vol. 94, no. 1–2. Elsevier,
    pp. 96–104, 2001.
  ista: Jerecic J, Schulze C, Jonas PM, Sprengel R, Seeburg P, Bischofberger J. 2001.
    Impaired NMDA receptor function in mouse olfactory bulb neurons by tetracycline-sensitive
    NR1 (N598R) expression. Molecular Brain Research. 94(1–2), 96–104.
  mla: Jerecic, Jasna, et al. “Impaired NMDA Receptor Function in Mouse Olfactory
    Bulb Neurons by Tetracycline-Sensitive NR1 (N598R) Expression.” <i>Molecular Brain
    Research</i>, vol. 94, no. 1–2, Elsevier, 2001, pp. 96–104, doi:<a href="https://doi.org/10.1016/S0169-328X(01)00221-2">10.1016/S0169-328X(01)00221-2</a>.
  short: J. Jerecic, C. Schulze, P.M. Jonas, R. Sprengel, P. Seeburg, J. Bischofberger,
    Molecular Brain Research 94 (2001) 96–104.
date_created: 2018-12-11T12:03:38Z
date_published: 2001-10-19T00:00:00Z
date_updated: 2023-05-15T13:42:32Z
day: '19'
doi: 10.1016/S0169-328X(01)00221-2
extern: '1'
external_id:
  pmid:
  - '11597769'
intvolume: '        94'
issue: 1-2
language:
- iso: eng
month: '10'
oa_version: None
page: 96 - 104
pmid: 1
publication: Molecular Brain Research
publication_identifier:
  issn:
  - 0169-328X
publication_status: published
publisher: Elsevier
publist_id: '2892'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Impaired NMDA receptor function in mouse olfactory bulb neurons by tetracycline-sensitive
  NR1 (N598R) expression
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 94
year: '2001'
...