---
_id: '2600'
abstract:
- lang: eng
  text: 'The synaptic relationship between substance P (SP) and its receptor, i.e.
    neurokinin-1 receptor (NK1R), was examined in the superficial laminae of the caudal
    subnucleus of the spinal trigeminal nucleus (medullary dorsal horn; MDH) of the
    rat. For confocal laser-scanning microscopy, double-immunofluorescence histochemistry
    for NK1 and SP was performed. In electron microscopic double-immunolabeling study,
    immunoreactivity for NK1R was detected with the silver-intensified gold method,
    while immunoreactivity for SP was detected with peroxidase immunohistochemistry.
    SP-immunoreactive axon terminals were observed to be in synaptic (mostly asymmetric)
    contact with NK1R-immunoreactive neuronal profiles in lamina I and lamina IIo.
    Although some SP-immunoreactive axon terminals were in synaptic contact with NK1R-immunoreactive
    sites of plasma membranes, NK1R-immunoreactivity was observed at both synaptic
    and non-synaptic sites of plasma membrane. Thus, SP released from axon terminals
    might not only act on NK1Rs facing the SP-containing axon terminals, but also
    diffuse in the extracellular fluid for distances larger than the synaptic cleft
    to act on NK1Rs at some distances from the synaptic sites. '
article_processing_charge: No
article_type: original
author:
- first_name: Jin
  full_name: Li, Jin
  last_name: Li
- first_name: Dan
  full_name: Wang, Dan
  last_name: Wang
- first_name: Takeshi
  full_name: Kaneko, Takeshi
  last_name: Kaneko
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Sakashi
  full_name: Nomura, Sakashi
  last_name: Nomura
- first_name: Noboru
  full_name: Mizuno, Noboru
  last_name: Mizuno
citation:
  ama: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. The relationship
    between neurokinin-1 receptor and substance P in the medullary dorsal horn: A
    light and electron microscopic immunohistochemical study in the rat. <i>Neuroscience
    Research</i>. 2000;36(4):327-334. doi:<a href="https://doi.org/10.1016/S0168-0102(00)00095-X">10.1016/S0168-0102(00)00095-X</a>'
  apa: 'Li, J., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., &#38; Mizuno, N.
    (2000). The relationship between neurokinin-1 receptor and substance P in the
    medullary dorsal horn: A light and electron microscopic immunohistochemical study
    in the rat. <i>Neuroscience Research</i>. Elsevier. <a href="https://doi.org/10.1016/S0168-0102(00)00095-X">https://doi.org/10.1016/S0168-0102(00)00095-X</a>'
  chicago: 'Li, Jin, Dan Wang, Takeshi Kaneko, Ryuichi Shigemoto, Sakashi Nomura,
    and Noboru Mizuno. “The Relationship between Neurokinin-1 Receptor and Substance
    P in the Medullary Dorsal Horn: A Light and Electron Microscopic Immunohistochemical
    Study in the Rat.” <i>Neuroscience Research</i>. Elsevier, 2000. <a href="https://doi.org/10.1016/S0168-0102(00)00095-X">https://doi.org/10.1016/S0168-0102(00)00095-X</a>.'
  ieee: 'J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, and N. Mizuno, “The relationship
    between neurokinin-1 receptor and substance P in the medullary dorsal horn: A
    light and electron microscopic immunohistochemical study in the rat,” <i>Neuroscience
    Research</i>, vol. 36, no. 4. Elsevier, pp. 327–334, 2000.'
  ista: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. 2000. The relationship
    between neurokinin-1 receptor and substance P in the medullary dorsal horn: A
    light and electron microscopic immunohistochemical study in the rat. Neuroscience
    Research. 36(4), 327–334.'
  mla: 'Li, Jin, et al. “The Relationship between Neurokinin-1 Receptor and Substance
    P in the Medullary Dorsal Horn: A Light and Electron Microscopic Immunohistochemical
    Study in the Rat.” <i>Neuroscience Research</i>, vol. 36, no. 4, Elsevier, 2000,
    pp. 327–34, doi:<a href="https://doi.org/10.1016/S0168-0102(00)00095-X">10.1016/S0168-0102(00)00095-X</a>.'
  short: J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, N. Mizuno, Neuroscience
    Research 36 (2000) 327–334.
date_created: 2018-12-11T11:58:36Z
date_published: 2000-04-01T00:00:00Z
date_updated: 2023-05-03T10:47:06Z
day: '01'
doi: 10.1016/S0168-0102(00)00095-X
extern: '1'
external_id:
  pmid:
  - '10771111'
intvolume: '        36'
issue: '4'
language:
- iso: eng
month: '04'
oa_version: None
page: 327 - 334
pmid: 1
publication: Neuroscience Research
publication_identifier:
  issn:
  - 0168-0102
publication_status: published
publisher: Elsevier
publist_id: '4298'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'The relationship between neurokinin-1 receptor and substance P in the medullary
  dorsal horn: A light and electron microscopic immunohistochemical study in the rat'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 36
year: '2000'
...
---
_id: '2583'
abstract:
- lang: eng
  text: Substance P receptor (SPR)-immunoreactive neurons projecting to the periaqueductal
    gray (PAG) were examined in the rat spinal trigeminal nucleus and spinal cord
    by a retrograde tracing method combined with immunofluorescence histochemistry.
    After injection of Fluoro-gold (FG) into the PAG, SPR-immunoreactive neurons labeled
    with FG were observed mainly in the lateral spinal nucleus and lamina I of the
    medullary and spinal dorsal horns and additionally in laminae V and X of the spinal
    cord.
acknowledgement: "The authors are grateful for the support of Dr Kajitaro Morita of
  the Morita Clinic of Internal Medicine and Pediatrics at Kadoma, Osaka and for the
  help of Yue-Ping Yuan and Akira Uesugi with photography. This work was supported
  in part by Grants-in-Aid from the National Natural Science Foundation of China (39600045)
  and the Ministry of Education, Sci-\r\nence, Sports and Culture of Japan (09480211,
  08458245)."
article_processing_charge: No
article_type: original
author:
- first_name: Jin
  full_name: Li, Jin
  last_name: Li
- first_name: Yu
  full_name: Ding, Yu
  last_name: Ding
- first_name: Kang
  full_name: Xiong, Kang
  last_name: Xiong
- first_name: Ji
  full_name: Li, Ji
  last_name: Li
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Noboru
  full_name: Mizuno, Noboru
  last_name: Mizuno
citation:
  ama: 'Li J, Ding Y, Xiong K, Li J, Shigemoto R, Mizuno N. Substance P receptor (NK1)-immunoreactive
    neurons projecting to the periaqueductal gray: Distribution in the spinal trigeminal
    nucleus and the spinal cord of the rat. <i>Neuroscience Research</i>. 1998;30(3):219-225.
    doi:<a href="https://doi.org/10.1016/S0168-0102(97)00132-6">10.1016/S0168-0102(97)00132-6</a>'
  apa: 'Li, J., Ding, Y., Xiong, K., Li, J., Shigemoto, R., &#38; Mizuno, N. (1998).
    Substance P receptor (NK1)-immunoreactive neurons projecting to the periaqueductal
    gray: Distribution in the spinal trigeminal nucleus and the spinal cord of the
    rat. <i>Neuroscience Research</i>. Elsevier. <a href="https://doi.org/10.1016/S0168-0102(97)00132-6">https://doi.org/10.1016/S0168-0102(97)00132-6</a>'
  chicago: 'Li, Jin, Yu Ding, Kang Xiong, Ji Li, Ryuichi Shigemoto, and Noboru Mizuno.
    “Substance P Receptor (NK1)-Immunoreactive Neurons Projecting to the Periaqueductal
    Gray: Distribution in the Spinal Trigeminal Nucleus and the Spinal Cord of the
    Rat.” <i>Neuroscience Research</i>. Elsevier, 1998. <a href="https://doi.org/10.1016/S0168-0102(97)00132-6">https://doi.org/10.1016/S0168-0102(97)00132-6</a>.'
  ieee: 'J. Li, Y. Ding, K. Xiong, J. Li, R. Shigemoto, and N. Mizuno, “Substance
    P receptor (NK1)-immunoreactive neurons projecting to the periaqueductal gray:
    Distribution in the spinal trigeminal nucleus and the spinal cord of the rat,”
    <i>Neuroscience Research</i>, vol. 30, no. 3. Elsevier, pp. 219–225, 1998.'
  ista: 'Li J, Ding Y, Xiong K, Li J, Shigemoto R, Mizuno N. 1998. Substance P receptor
    (NK1)-immunoreactive neurons projecting to the periaqueductal gray: Distribution
    in the spinal trigeminal nucleus and the spinal cord of the rat. Neuroscience
    Research. 30(3), 219–225.'
  mla: 'Li, Jin, et al. “Substance P Receptor (NK1)-Immunoreactive Neurons Projecting
    to the Periaqueductal Gray: Distribution in the Spinal Trigeminal Nucleus and
    the Spinal Cord of the Rat.” <i>Neuroscience Research</i>, vol. 30, no. 3, Elsevier,
    1998, pp. 219–25, doi:<a href="https://doi.org/10.1016/S0168-0102(97)00132-6">10.1016/S0168-0102(97)00132-6</a>.'
  short: J. Li, Y. Ding, K. Xiong, J. Li, R. Shigemoto, N. Mizuno, Neuroscience Research
    30 (1998) 219–225.
date_created: 2018-12-11T11:58:31Z
date_published: 1998-03-01T00:00:00Z
date_updated: 2022-09-01T12:19:44Z
day: '01'
doi: 10.1016/S0168-0102(97)00132-6
extern: '1'
external_id:
  pmid:
  - '9593332'
intvolume: '        30'
issue: '3'
language:
- iso: eng
month: '03'
oa_version: None
page: 219 - 225
pmid: 1
publication: Neuroscience Research
publication_identifier:
  issn:
  - 0168-0102
publication_status: published
publisher: Elsevier
publist_id: '4315'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Substance P receptor (NK1)-immunoreactive neurons projecting to the periaqueductal
  gray: Distribution in the spinal trigeminal nucleus and the spinal cord of the rat'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 30
year: '1998'
...
---
_id: '2563'
abstract:
- lang: eng
  text: By means of substance P receptor (SPR) immunofluorescence histochemistry combined
    with Fluoro-Gold fluorescent retrograde labeling, SPR-like immunoreactive neurons
    in the caudal subnucleus of the spinal trigeminal nucleus of the rat were observed
    to send their axons to the nucleus of Kolliker-Fuse and ventrolateral part of
    the lateral parabrachial nucleus bilaterally with a clear ipsilateral dominance.
    These neurons were distributed mainly in lamina I, and additionally in lamina
    III.
acknowledgement: 'The authors are grateful for photographic help of Ms. Miao-Li Zhang
  and Mr. Akira Uesugi. This work was supported in part by Grants-in-Aid for Special
  Research on Priority Areas 05267104, Scientific Research (B) 05454658, and Scientific
  Research (C) 06680735 from the Ministry of Education, Science and Culture of Japan. '
article_processing_charge: No
article_type: original
author:
- first_name: Yu
  full_name: Ding, Yu
  last_name: Ding
- first_name: Masahiko
  full_name: Takada, Masahiko
  last_name: Takada
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Noboru
  full_name: Mizuno, Noboru
  last_name: Mizuno
citation:
  ama: Ding Y, Takada M, Shigemoto R, Mizuno N. Trigeminoparabrachial projection neurons
    showing substance P receptor-like immunoreactivity in the rat. <i>Neuroscience
    Research</i>. 1995;23(4):415-418. doi:<a href="https://doi.org/10.1016/0168-0102(95)00961-R">10.1016/0168-0102(95)00961-R</a>
  apa: Ding, Y., Takada, M., Shigemoto, R., &#38; Mizuno, N. (1995). Trigeminoparabrachial
    projection neurons showing substance P receptor-like immunoreactivity in the rat.
    <i>Neuroscience Research</i>. Elsevier. <a href="https://doi.org/10.1016/0168-0102(95)00961-R">https://doi.org/10.1016/0168-0102(95)00961-R</a>
  chicago: Ding, Yu, Masahiko Takada, Ryuichi Shigemoto, and Noboru Mizuno. “Trigeminoparabrachial
    Projection Neurons Showing Substance P Receptor-like Immunoreactivity in the Rat.”
    <i>Neuroscience Research</i>. Elsevier, 1995. <a href="https://doi.org/10.1016/0168-0102(95)00961-R">https://doi.org/10.1016/0168-0102(95)00961-R</a>.
  ieee: Y. Ding, M. Takada, R. Shigemoto, and N. Mizuno, “Trigeminoparabrachial projection
    neurons showing substance P receptor-like immunoreactivity in the rat,” <i>Neuroscience
    Research</i>, vol. 23, no. 4. Elsevier, pp. 415–418, 1995.
  ista: Ding Y, Takada M, Shigemoto R, Mizuno N. 1995. Trigeminoparabrachial projection
    neurons showing substance P receptor-like immunoreactivity in the rat. Neuroscience
    Research. 23(4), 415–418.
  mla: Ding, Yu, et al. “Trigeminoparabrachial Projection Neurons Showing Substance
    P Receptor-like Immunoreactivity in the Rat.” <i>Neuroscience Research</i>, vol.
    23, no. 4, Elsevier, 1995, pp. 415–18, doi:<a href="https://doi.org/10.1016/0168-0102(95)00961-R">10.1016/0168-0102(95)00961-R</a>.
  short: Y. Ding, M. Takada, R. Shigemoto, N. Mizuno, Neuroscience Research 23 (1995)
    415–418.
date_created: 2018-12-11T11:58:24Z
date_published: 1995-11-01T00:00:00Z
date_updated: 2022-06-28T09:50:42Z
day: '01'
doi: 10.1016/0168-0102(95)00961-R
extern: '1'
external_id:
  pmid:
  - '8602281'
intvolume: '        23'
issue: '4'
language:
- iso: eng
main_file_link:
- url: https://www.sciencedirect.com/science/article/pii/016801029500961R?via%3Dihub
month: '11'
oa_version: None
page: 415 - 418
pmid: 1
publication: Neuroscience Research
publication_identifier:
  issn:
  - 0168-0102
publication_status: published
publisher: Elsevier
publist_id: '4336'
quality_controlled: '1'
status: public
title: Trigeminoparabrachial projection neurons showing substance P receptor-like
  immunoreactivity in the rat
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 23
year: '1995'
...