[{"abstract":[{"text":"The synaptic relationship between substance P (SP) and its receptor, i.e. neurokinin-1 receptor (NK1R), was examined in the superficial laminae of the caudal subnucleus of the spinal trigeminal nucleus (medullary dorsal horn; MDH) of the rat. For confocal laser-scanning microscopy, double-immunofluorescence histochemistry for NK1 and SP was performed. In electron microscopic double-immunolabeling study, immunoreactivity for NK1R was detected with the silver-intensified gold method, while immunoreactivity for SP was detected with peroxidase immunohistochemistry. SP-immunoreactive axon terminals were observed to be in synaptic (mostly asymmetric) contact with NK1R-immunoreactive neuronal profiles in lamina I and lamina IIo. Although some SP-immunoreactive axon terminals were in synaptic contact with NK1R-immunoreactive sites of plasma membranes, NK1R-immunoreactivity was observed at both synaptic and non-synaptic sites of plasma membrane. Thus, SP released from axon terminals might not only act on NK1Rs facing the SP-containing axon terminals, but also diffuse in the extracellular fluid for distances larger than the synaptic cleft to act on NK1Rs at some distances from the synaptic sites. ","lang":"eng"}],"doi":"10.1016/S0168-0102(00)00095-X","day":"01","external_id":{"pmid":["10771111"]},"date_updated":"2023-05-03T10:47:06Z","citation":{"ista":"Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. 2000. The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat. Neuroscience Research. 36(4), 327–334.","mla":"Li, Jin, et al. “The Relationship between Neurokinin-1 Receptor and Substance P in the Medullary Dorsal Horn: A Light and Electron Microscopic Immunohistochemical Study in the Rat.” <i>Neuroscience Research</i>, vol. 36, no. 4, Elsevier, 2000, pp. 327–34, doi:<a href=\"https://doi.org/10.1016/S0168-0102(00)00095-X\">10.1016/S0168-0102(00)00095-X</a>.","short":"J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, N. Mizuno, Neuroscience Research 36 (2000) 327–334.","chicago":"Li, Jin, Dan Wang, Takeshi Kaneko, Ryuichi Shigemoto, Sakashi Nomura, and Noboru Mizuno. “The Relationship between Neurokinin-1 Receptor and Substance P in the Medullary Dorsal Horn: A Light and Electron Microscopic Immunohistochemical Study in the Rat.” <i>Neuroscience Research</i>. Elsevier, 2000. <a href=\"https://doi.org/10.1016/S0168-0102(00)00095-X\">https://doi.org/10.1016/S0168-0102(00)00095-X</a>.","ieee":"J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, and N. Mizuno, “The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat,” <i>Neuroscience Research</i>, vol. 36, no. 4. Elsevier, pp. 327–334, 2000.","apa":"Li, J., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., &#38; Mizuno, N. (2000). The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat. <i>Neuroscience Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/S0168-0102(00)00095-X\">https://doi.org/10.1016/S0168-0102(00)00095-X</a>","ama":"Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat. <i>Neuroscience Research</i>. 2000;36(4):327-334. doi:<a href=\"https://doi.org/10.1016/S0168-0102(00)00095-X\">10.1016/S0168-0102(00)00095-X</a>"},"year":"2000","extern":"1","volume":36,"title":"The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat","intvolume":"        36","publication_status":"published","date_created":"2018-12-11T11:58:36Z","article_processing_charge":"No","author":[{"first_name":"Jin","last_name":"Li","full_name":"Li, Jin"},{"full_name":"Wang, Dan","first_name":"Dan","last_name":"Wang"},{"last_name":"Kaneko","first_name":"Takeshi","full_name":"Kaneko, Takeshi"},{"full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","last_name":"Shigemoto","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Sakashi","last_name":"Nomura","full_name":"Nomura, Sakashi"},{"last_name":"Mizuno","first_name":"Noboru","full_name":"Mizuno, Noboru"}],"issue":"4","pmid":1,"_id":"2600","scopus_import":"1","article_type":"original","publisher":"Elsevier","page":"327 - 334","quality_controlled":"1","publist_id":"4298","publication_identifier":{"issn":["0168-0102"]},"date_published":"2000-04-01T00:00:00Z","type":"journal_article","status":"public","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","month":"04","oa_version":"None","publication":"Neuroscience Research","language":[{"iso":"eng"}]},{"publisher":"Elsevier","article_type":"original","page":"219 - 225","quality_controlled":"1","publication_status":"published","date_created":"2018-12-11T11:58:31Z","article_processing_charge":"No","title":"Substance P receptor (NK1)-immunoreactive neurons projecting to the periaqueductal gray: Distribution in the spinal trigeminal nucleus and the spinal cord of the rat","intvolume":"        30","pmid":1,"_id":"2583","scopus_import":"1","author":[{"full_name":"Li, Jin","last_name":"Li","first_name":"Jin"},{"full_name":"Ding, Yu","last_name":"Ding","first_name":"Yu"},{"full_name":"Xiong, Kang","first_name":"Kang","last_name":"Xiong"},{"last_name":"Li","first_name":"Ji","full_name":"Li, Ji"},{"id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","last_name":"Shigemoto","first_name":"Ryuichi"},{"full_name":"Mizuno, Noboru","first_name":"Noboru","last_name":"Mizuno"}],"issue":"3","volume":30,"acknowledgement":"The authors are grateful for the support of Dr Kajitaro Morita of the Morita Clinic of Internal Medicine and Pediatrics at Kadoma, Osaka and for the help of Yue-Ping Yuan and Akira Uesugi with photography. This work was supported in part by Grants-in-Aid from the National Natural Science Foundation of China (39600045) and the Ministry of Education, Sci-\r\nence, Sports and Culture of Japan (09480211, 08458245).","extern":"1","doi":"10.1016/S0168-0102(97)00132-6","day":"01","abstract":[{"text":"Substance P receptor (SPR)-immunoreactive neurons projecting to the periaqueductal gray (PAG) were examined in the rat spinal trigeminal nucleus and spinal cord by a retrograde tracing method combined with immunofluorescence histochemistry. After injection of Fluoro-gold (FG) into the PAG, SPR-immunoreactive neurons labeled with FG were observed mainly in the lateral spinal nucleus and lamina I of the medullary and spinal dorsal horns and additionally in laminae V and X of the spinal cord.","lang":"eng"}],"date_updated":"2022-09-01T12:19:44Z","citation":{"ieee":"J. Li, Y. Ding, K. Xiong, J. Li, R. Shigemoto, and N. Mizuno, “Substance P receptor (NK1)-immunoreactive neurons projecting to the periaqueductal gray: Distribution in the spinal trigeminal nucleus and the spinal cord of the rat,” <i>Neuroscience Research</i>, vol. 30, no. 3. Elsevier, pp. 219–225, 1998.","chicago":"Li, Jin, Yu Ding, Kang Xiong, Ji Li, Ryuichi Shigemoto, and Noboru Mizuno. “Substance P Receptor (NK1)-Immunoreactive Neurons Projecting to the Periaqueductal Gray: Distribution in the Spinal Trigeminal Nucleus and the Spinal Cord of the Rat.” <i>Neuroscience Research</i>. Elsevier, 1998. <a href=\"https://doi.org/10.1016/S0168-0102(97)00132-6\">https://doi.org/10.1016/S0168-0102(97)00132-6</a>.","apa":"Li, J., Ding, Y., Xiong, K., Li, J., Shigemoto, R., &#38; Mizuno, N. (1998). Substance P receptor (NK1)-immunoreactive neurons projecting to the periaqueductal gray: Distribution in the spinal trigeminal nucleus and the spinal cord of the rat. <i>Neuroscience Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/S0168-0102(97)00132-6\">https://doi.org/10.1016/S0168-0102(97)00132-6</a>","ama":"Li J, Ding Y, Xiong K, Li J, Shigemoto R, Mizuno N. Substance P receptor (NK1)-immunoreactive neurons projecting to the periaqueductal gray: Distribution in the spinal trigeminal nucleus and the spinal cord of the rat. <i>Neuroscience Research</i>. 1998;30(3):219-225. doi:<a href=\"https://doi.org/10.1016/S0168-0102(97)00132-6\">10.1016/S0168-0102(97)00132-6</a>","ista":"Li J, Ding Y, Xiong K, Li J, Shigemoto R, Mizuno N. 1998. Substance P receptor (NK1)-immunoreactive neurons projecting to the periaqueductal gray: Distribution in the spinal trigeminal nucleus and the spinal cord of the rat. Neuroscience Research. 30(3), 219–225.","short":"J. Li, Y. Ding, K. Xiong, J. Li, R. Shigemoto, N. Mizuno, Neuroscience Research 30 (1998) 219–225.","mla":"Li, Jin, et al. “Substance P Receptor (NK1)-Immunoreactive Neurons Projecting to the Periaqueductal Gray: Distribution in the Spinal Trigeminal Nucleus and the Spinal Cord of the Rat.” <i>Neuroscience Research</i>, vol. 30, no. 3, Elsevier, 1998, pp. 219–25, doi:<a href=\"https://doi.org/10.1016/S0168-0102(97)00132-6\">10.1016/S0168-0102(97)00132-6</a>."},"year":"1998","external_id":{"pmid":["9593332"]},"language":[{"iso":"eng"}],"oa_version":"None","month":"03","publication":"Neuroscience Research","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","status":"public","publication_identifier":{"issn":["0168-0102"]},"publist_id":"4315","date_published":"1998-03-01T00:00:00Z","type":"journal_article"},{"publication":"Neuroscience Research","oa_version":"None","month":"11","language":[{"iso":"eng"}],"type":"journal_article","date_published":"1995-11-01T00:00:00Z","publication_identifier":{"issn":["0168-0102"]},"publist_id":"4336","main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/016801029500961R?via%3Dihub"}],"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","status":"public","pmid":1,"_id":"2563","issue":"4","author":[{"first_name":"Yu","last_name":"Ding","full_name":"Ding, Yu"},{"full_name":"Takada, Masahiko","first_name":"Masahiko","last_name":"Takada"},{"id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","first_name":"Ryuichi","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444"},{"first_name":"Noboru","last_name":"Mizuno","full_name":"Mizuno, Noboru"}],"date_created":"2018-12-11T11:58:24Z","article_processing_charge":"No","publication_status":"published","intvolume":"        23","title":"Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat","quality_controlled":"1","page":"415 - 418","publisher":"Elsevier","article_type":"original","year":"1995","citation":{"ista":"Ding Y, Takada M, Shigemoto R, Mizuno N. 1995. Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat. Neuroscience Research. 23(4), 415–418.","short":"Y. Ding, M. Takada, R. Shigemoto, N. Mizuno, Neuroscience Research 23 (1995) 415–418.","mla":"Ding, Yu, et al. “Trigeminoparabrachial Projection Neurons Showing Substance P Receptor-like Immunoreactivity in the Rat.” <i>Neuroscience Research</i>, vol. 23, no. 4, Elsevier, 1995, pp. 415–18, doi:<a href=\"https://doi.org/10.1016/0168-0102(95)00961-R\">10.1016/0168-0102(95)00961-R</a>.","chicago":"Ding, Yu, Masahiko Takada, Ryuichi Shigemoto, and Noboru Mizuno. “Trigeminoparabrachial Projection Neurons Showing Substance P Receptor-like Immunoreactivity in the Rat.” <i>Neuroscience Research</i>. Elsevier, 1995. <a href=\"https://doi.org/10.1016/0168-0102(95)00961-R\">https://doi.org/10.1016/0168-0102(95)00961-R</a>.","ieee":"Y. Ding, M. Takada, R. Shigemoto, and N. Mizuno, “Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat,” <i>Neuroscience Research</i>, vol. 23, no. 4. Elsevier, pp. 415–418, 1995.","ama":"Ding Y, Takada M, Shigemoto R, Mizuno N. Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat. <i>Neuroscience Research</i>. 1995;23(4):415-418. doi:<a href=\"https://doi.org/10.1016/0168-0102(95)00961-R\">10.1016/0168-0102(95)00961-R</a>","apa":"Ding, Y., Takada, M., Shigemoto, R., &#38; Mizuno, N. (1995). Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat. <i>Neuroscience Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/0168-0102(95)00961-R\">https://doi.org/10.1016/0168-0102(95)00961-R</a>"},"date_updated":"2022-06-28T09:50:42Z","external_id":{"pmid":["8602281"]},"day":"01","doi":"10.1016/0168-0102(95)00961-R","abstract":[{"lang":"eng","text":"By means of substance P receptor (SPR) immunofluorescence histochemistry combined with Fluoro-Gold fluorescent retrograde labeling, SPR-like immunoreactive neurons in the caudal subnucleus of the spinal trigeminal nucleus of the rat were observed to send their axons to the nucleus of Kolliker-Fuse and ventrolateral part of the lateral parabrachial nucleus bilaterally with a clear ipsilateral dominance. These neurons were distributed mainly in lamina I, and additionally in lamina III."}],"acknowledgement":"The authors are grateful for photographic help of Ms. Miao-Li Zhang and Mr. Akira Uesugi. This work was supported in part by Grants-in-Aid for Special Research on Priority Areas 05267104, Scientific Research (B) 05454658, and Scientific Research (C) 06680735 from the Ministry of Education, Science and Culture of Japan. ","volume":23,"extern":"1"}]