@article{2600,
  abstract     = {The synaptic relationship between substance P (SP) and its receptor, i.e. neurokinin-1 receptor (NK1R), was examined in the superficial laminae of the caudal subnucleus of the spinal trigeminal nucleus (medullary dorsal horn; MDH) of the rat. For confocal laser-scanning microscopy, double-immunofluorescence histochemistry for NK1 and SP was performed. In electron microscopic double-immunolabeling study, immunoreactivity for NK1R was detected with the silver-intensified gold method, while immunoreactivity for SP was detected with peroxidase immunohistochemistry. SP-immunoreactive axon terminals were observed to be in synaptic (mostly asymmetric) contact with NK1R-immunoreactive neuronal profiles in lamina I and lamina IIo. Although some SP-immunoreactive axon terminals were in synaptic contact with NK1R-immunoreactive sites of plasma membranes, NK1R-immunoreactivity was observed at both synaptic and non-synaptic sites of plasma membrane. Thus, SP released from axon terminals might not only act on NK1Rs facing the SP-containing axon terminals, but also diffuse in the extracellular fluid for distances larger than the synaptic cleft to act on NK1Rs at some distances from the synaptic sites. },
  author       = {Li, Jin and Wang, Dan and Kaneko, Takeshi and Shigemoto, Ryuichi and Nomura, Sakashi and Mizuno, Noboru},
  issn         = {0168-0102},
  journal      = {Neuroscience Research},
  number       = {4},
  pages        = {327 -- 334},
  publisher    = {Elsevier},
  title        = {{The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat}},
  doi          = {10.1016/S0168-0102(00)00095-X},
  volume       = {36},
  year         = {2000},
}

@article{2583,
  abstract     = {Substance P receptor (SPR)-immunoreactive neurons projecting to the periaqueductal gray (PAG) were examined in the rat spinal trigeminal nucleus and spinal cord by a retrograde tracing method combined with immunofluorescence histochemistry. After injection of Fluoro-gold (FG) into the PAG, SPR-immunoreactive neurons labeled with FG were observed mainly in the lateral spinal nucleus and lamina I of the medullary and spinal dorsal horns and additionally in laminae V and X of the spinal cord.},
  author       = {Li, Jin and Ding, Yu and Xiong, Kang and Li, Ji and Shigemoto, Ryuichi and Mizuno, Noboru},
  issn         = {0168-0102},
  journal      = {Neuroscience Research},
  number       = {3},
  pages        = {219 -- 225},
  publisher    = {Elsevier},
  title        = {{Substance P receptor (NK1)-immunoreactive neurons projecting to the periaqueductal gray: Distribution in the spinal trigeminal nucleus and the spinal cord of the rat}},
  doi          = {10.1016/S0168-0102(97)00132-6},
  volume       = {30},
  year         = {1998},
}

@article{2563,
  abstract     = {By means of substance P receptor (SPR) immunofluorescence histochemistry combined with Fluoro-Gold fluorescent retrograde labeling, SPR-like immunoreactive neurons in the caudal subnucleus of the spinal trigeminal nucleus of the rat were observed to send their axons to the nucleus of Kolliker-Fuse and ventrolateral part of the lateral parabrachial nucleus bilaterally with a clear ipsilateral dominance. These neurons were distributed mainly in lamina I, and additionally in lamina III.},
  author       = {Ding, Yu and Takada, Masahiko and Shigemoto, Ryuichi and Mizuno, Noboru},
  issn         = {0168-0102},
  journal      = {Neuroscience Research},
  number       = {4},
  pages        = {415 -- 418},
  publisher    = {Elsevier},
  title        = {{Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat}},
  doi          = {10.1016/0168-0102(95)00961-R},
  volume       = {23},
  year         = {1995},
}