---
_id: '8233'
abstract:
- lang: eng
  text: The M2a subtype of macrophages plays an important role in human immunoglobulin
    E (IgE-mediated allergies) and other Th2 type immune reactions. In contrast, very
    little is known about these cells in the dog. Here we describe an in vitro method
    to activate canine histiocytic DH82 cells and primary canine monocyte-derived
    macrophages (MDMs) toward the M2a macrophages using human cytokines. For a side-by-side
    comparison, we compared the canine cells to human MDMs, and the human monocytic
    cell line U937 activated towards M1 and M2a cells on the cellular and molecular
    level. In analogy to activated human M2a cells, canine M2a, differentiated from
    both DH82 and MDMs, showed an increase in CD206 surface receptor expression compared
    to M1. Interestingly, canine M2a, but not M1 derived from MDM, upregulated the
    high-affinity IgE receptor (FcεRI). Transcription levels of M2a-associated genes
    (IL10, CCL22, TGFβ, CD163) showed a diverse pattern between the human and dog
    species, whereas M1 genes (IDO1, CXCL11, IL6, TNF-α) were similarly upregulated
    in canine and human M1 cells (cell lines and MDMs). We suggest that our novel
    in vitro method will be suitable in comparative allergology studies focussing
    on macrophages.
article_processing_charge: No
article_type: original
author:
- first_name: Ina
  full_name: Herrmann, Ina
  last_name: Herrmann
- first_name: Jelena
  full_name: Gotovina, Jelena
  last_name: Gotovina
- first_name: Judit
  full_name: Fazekas-Singer, Judit
  id: 36432834-F248-11E8-B48F-1D18A9856A87
  last_name: Fazekas-Singer
  orcid: 0000-0002-8777-3502
- first_name: Michael B.
  full_name: Fischer, Michael B.
  last_name: Fischer
- first_name: Karin
  full_name: Hufnagl, Karin
  last_name: Hufnagl
- first_name: Rodolfo
  full_name: Bianchini, Rodolfo
  last_name: Bianchini
- first_name: Erika
  full_name: Jensen-Jarolim, Erika
  last_name: Jensen-Jarolim
citation:
  ama: Herrmann I, Gotovina J, Singer J, et al. Canine macrophages can like human
    macrophages be in vitro activated toward the M2a subtype relevant in allergy.
    <i>Developmental &#38; Comparative Immunology</i>. 2018;82(5):118-127. doi:<a
    href="https://doi.org/10.1016/j.dci.2018.01.005">10.1016/j.dci.2018.01.005</a>
  apa: Herrmann, I., Gotovina, J., Singer, J., Fischer, M. B., Hufnagl, K., Bianchini,
    R., &#38; Jensen-Jarolim, E. (2018). Canine macrophages can like human macrophages
    be in vitro activated toward the M2a subtype relevant in allergy. <i>Developmental
    &#38; Comparative Immunology</i>. Elsevier. <a href="https://doi.org/10.1016/j.dci.2018.01.005">https://doi.org/10.1016/j.dci.2018.01.005</a>
  chicago: Herrmann, Ina, Jelena Gotovina, Judit Singer, Michael B. Fischer, Karin
    Hufnagl, Rodolfo Bianchini, and Erika Jensen-Jarolim. “Canine Macrophages Can
    like Human Macrophages Be in Vitro Activated toward the M2a Subtype Relevant in
    Allergy.” <i>Developmental &#38; Comparative Immunology</i>. Elsevier, 2018. <a
    href="https://doi.org/10.1016/j.dci.2018.01.005">https://doi.org/10.1016/j.dci.2018.01.005</a>.
  ieee: I. Herrmann <i>et al.</i>, “Canine macrophages can like human macrophages
    be in vitro activated toward the M2a subtype relevant in allergy,” <i>Developmental
    &#38; Comparative Immunology</i>, vol. 82, no. 5. Elsevier, pp. 118–127, 2018.
  ista: Herrmann I, Gotovina J, Singer J, Fischer MB, Hufnagl K, Bianchini R, Jensen-Jarolim
    E. 2018. Canine macrophages can like human macrophages be in vitro activated toward
    the M2a subtype relevant in allergy. Developmental &#38; Comparative Immunology.
    82(5), 118–127.
  mla: Herrmann, Ina, et al. “Canine Macrophages Can like Human Macrophages Be in Vitro
    Activated toward the M2a Subtype Relevant in Allergy.” <i>Developmental &#38;
    Comparative Immunology</i>, vol. 82, no. 5, Elsevier, 2018, pp. 118–27, doi:<a
    href="https://doi.org/10.1016/j.dci.2018.01.005">10.1016/j.dci.2018.01.005</a>.
  short: I. Herrmann, J. Gotovina, J. Singer, M.B. Fischer, K. Hufnagl, R. Bianchini,
    E. Jensen-Jarolim, Developmental &#38; Comparative Immunology 82 (2018) 118–127.
date_created: 2020-08-10T11:53:01Z
date_published: 2018-05-01T00:00:00Z
date_updated: 2021-01-12T08:17:38Z
day: '01'
doi: 10.1016/j.dci.2018.01.005
extern: '1'
intvolume: '        82'
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.dci.2018.01.005
month: '05'
oa: 1
oa_version: Published Version
page: 118-127
publication: Developmental & Comparative Immunology
publication_identifier:
  issn:
  - 0145-305X
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: Canine macrophages can like human macrophages be in vitro activated toward
  the M2a subtype relevant in allergy
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 82
year: '2018'
...