---
_id: '5830'
abstract:
- lang: eng
  text: CLE peptides have been implicated in various developmental processes of plants
    and mediate their responses to environmental stimuli. However, the biological
    relevance of most CLE genes remains to be functionally characterized. Here, we
    report that CLE9, which is expressed in stomata, acts as an essential regulator
    in the induction of stomatal closure. Exogenous application of CLE9 peptides or
    overexpression of CLE9 effectively led to stomatal closure and enhanced drought
    tolerance, whereas CLE9 loss-of-function mutants were sensitivity to drought stress.
    CLE9-induced stomatal closure was impaired in abscisic acid (ABA)-deficient mutants,
    indicating that ABA is required for CLE9-medaited guard cell signalling. We further
    deciphered that two guard cell ABA-signalling components, OST1 and SLAC1, were
    responsible for CLE9-induced stomatal closure. MPK3 and MPK6 were activated by
    the CLE9 peptide, and CLE9 peptides failed to close stomata in mpk3 and mpk6 mutants.
    In addition, CLE9 peptides stimulated the induction of hydrogen peroxide (H2O2)
    and nitric oxide (NO) synthesis associated with stomatal closure, which was abolished
    in the NADPH oxidase-deficient mutants or nitric reductase mutants, respectively.
    Collectively, our results reveal a novel ABA-dependent function of CLE9 in the
    regulation of stomatal apertures, thereby suggesting a potential role of CLE9
    in the stress acclimatization of plants.
article_processing_charge: No
author:
- first_name: Luosha
  full_name: Zhang, Luosha
  last_name: Zhang
- first_name: Xiong
  full_name: Shi, Xiong
  last_name: Shi
- first_name: Yutao
  full_name: Zhang, Yutao
  last_name: Zhang
- first_name: Jiajing
  full_name: Wang, Jiajing
  last_name: Wang
- first_name: Jingwei
  full_name: Yang, Jingwei
  last_name: Yang
- first_name: Takashi
  full_name: Ishida, Takashi
  last_name: Ishida
- first_name: Wenqian
  full_name: Jiang, Wenqian
  last_name: Jiang
- first_name: Xiangyu
  full_name: Han, Xiangyu
  last_name: Han
- first_name: Jingke
  full_name: Kang, Jingke
  last_name: Kang
- first_name: Xuening
  full_name: Wang, Xuening
  last_name: Wang
- first_name: Lixia
  full_name: Pan, Lixia
  last_name: Pan
- first_name: Shuo
  full_name: Lv, Shuo
  last_name: Lv
- first_name: Bing
  full_name: Cao, Bing
  last_name: Cao
- first_name: Yonghong
  full_name: Zhang, Yonghong
  last_name: Zhang
- first_name: Jinbin
  full_name: Wu, Jinbin
  last_name: Wu
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Zhubing
  full_name: Hu, Zhubing
  last_name: Hu
- first_name: Langjun
  full_name: Cui, Langjun
  last_name: Cui
- first_name: Shinichiro
  full_name: Sawa, Shinichiro
  last_name: Sawa
- first_name: Junmin
  full_name: He, Junmin
  last_name: He
- first_name: Guodong
  full_name: Wang, Guodong
  last_name: Wang
citation:
  ama: Zhang L, Shi X, Zhang Y, et al. CLE9 peptide-induced stomatal closure is mediated
    by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana.
    <i>Plant Cell and Environment</i>. 2018. doi:<a href="https://doi.org/10.1111/pce.13475">10.1111/pce.13475</a>
  apa: Zhang, L., Shi, X., Zhang, Y., Wang, J., Yang, J., Ishida, T., … Wang, G. (2018).
    CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen peroxide,
    and nitric oxide in arabidopsis thaliana. <i>Plant Cell and Environment</i>. Wiley.
    <a href="https://doi.org/10.1111/pce.13475">https://doi.org/10.1111/pce.13475</a>
  chicago: Zhang, Luosha, Xiong Shi, Yutao Zhang, Jiajing Wang, Jingwei Yang, Takashi
    Ishida, Wenqian Jiang, et al. “CLE9 Peptide-Induced Stomatal Closure Is Mediated
    by Abscisic Acid, Hydrogen Peroxide, and Nitric Oxide in Arabidopsis Thaliana.”
    <i>Plant Cell and Environment</i>. Wiley, 2018. <a href="https://doi.org/10.1111/pce.13475">https://doi.org/10.1111/pce.13475</a>.
  ieee: L. Zhang <i>et al.</i>, “CLE9 peptide-induced stomatal closure is mediated
    by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana,”
    <i>Plant Cell and Environment</i>. Wiley, 2018.
  ista: Zhang L, Shi X, Zhang Y, Wang J, Yang J, Ishida T, Jiang W, Han X, Kang J,
    Wang X, Pan L, Lv S, Cao B, Zhang Y, Wu J, Han H, Hu Z, Cui L, Sawa S, He J, Wang
    G. 2018. CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen
    peroxide, and nitric oxide in arabidopsis thaliana. Plant Cell and Environment.
  mla: Zhang, Luosha, et al. “CLE9 Peptide-Induced Stomatal Closure Is Mediated by
    Abscisic Acid, Hydrogen Peroxide, and Nitric Oxide in Arabidopsis Thaliana.” <i>Plant
    Cell and Environment</i>, Wiley, 2018, doi:<a href="https://doi.org/10.1111/pce.13475">10.1111/pce.13475</a>.
  short: L. Zhang, X. Shi, Y. Zhang, J. Wang, J. Yang, T. Ishida, W. Jiang, X. Han,
    J. Kang, X. Wang, L. Pan, S. Lv, B. Cao, Y. Zhang, J. Wu, H. Han, Z. Hu, L. Cui,
    S. Sawa, J. He, G. Wang, Plant Cell and Environment (2018).
date_created: 2019-01-13T22:59:11Z
date_published: 2018-10-31T00:00:00Z
date_updated: 2023-09-11T12:43:31Z
day: '31'
department:
- _id: JiFr
doi: 10.1111/pce.13475
external_id:
  isi:
  - '000459014800021'
  pmid:
  - '30378140'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pubmed/30378140
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Cell and Environment
publication_identifier:
  issn:
  - '01407791'
publication_status: epub_ahead
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen
  peroxide, and nitric oxide in arabidopsis thaliana
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...