---
_id: '10825'
abstract:
- lang: eng
text: In development, lineage segregation is coordinated in time and space. An important
example is the mammalian inner cell mass, in which the primitive endoderm (PrE,
founder of the yolk sac) physically segregates from the epiblast (EPI, founder
of the fetus). While the molecular requirements have been well studied, the physical
mechanisms determining spatial segregation between EPI and PrE remain elusive.
Here, we investigate the mechanical basis of EPI and PrE sorting. We find that
rather than the differences in static cell surface mechanical parameters as in
classical sorting models, it is the differences in surface fluctuations that robustly
ensure physical lineage sorting. These differential surface fluctuations systematically
correlate with differential cellular fluidity, which we propose together constitute
a non-equilibrium sorting mechanism for EPI and PrE lineages. By combining experiments
and modeling, we identify cell surface dynamics as a key factor orchestrating
the correct spatial segregation of the founder embryonic lineages.
acknowledgement: We are grateful to H. Niwa for Dox regulatable PB vector; G. Charras
for EzrinT567D cDNA; K. Jones for tdTomato ESCs, R26-Confetti ESCs, and laboratory
assistance; M. Kinoshita for pPB-CAG-H2B-BFP plasmid; P. Humphreys and D. Clements
for imaging support; G. Chu, P. Attlesey, and staff for animal husbandry; S. Pallett
for laboratory assistance; C. Mulas for critical feedback on the project; T. Boroviak
for single-cell RNA-seq; the EMBL Genomics Core Facility for sequencing; and M.
Merkel for developing and sharing the original version of the 3D Voronoi code. This
work was financially supported by BBSRC ( BB/Moo4023/1 and BB/T007044/1 to K.J.C.
and J.N., Alert16 grant BB/R000042 to E.K.P.), Leverhulme Trust ( RPG-2014-080 to
K.J.C. and J.N.), European Research Council ( 772798 -CellFateTech to K.J.C., 311637
-MorphoCorDiv and 820188 -NanoMechShape to E.K.P., Starting Grant 851288 to E.H.,
and 772426 -MeChemGui to K.F.), the Isaac Newton Trust (to E.K.P.), Medical Research
Council UK (MRC program award MC_UU_00012/5 to E.K.P.), the European Union’s Horizon
2020 research and innovation program under the Marie Sklodowska-Curie grant agreement
no. 641639 ( ITN Biopol , H.D.B. and E.K.P.), the Alexander von Humboldt Foundation
(Alexander von Humboldt Professorship to K.F.), EMBO ALTF 522-2021 (to P.S.), Centre
for Trophoblast Research (Next Generation fellowship to S.A.), and JSPS Overseas
Research Fellowships (to A.Y.). The Wellcome-MRC Cambridge Stem Cell Institute receives
core funding from Wellcome Trust ( 203151/Z/16/Z ) and MRC ( MC_PC_17230 ). For
the purpose of open access, the author has applied a CC BY public copyright licence
to any Author Accepted Manuscript version arising from this submission.
article_processing_charge: No
article_type: original
author:
- first_name: Ayaka
full_name: Yanagida, Ayaka
last_name: Yanagida
- first_name: Elena
full_name: Corujo-Simon, Elena
last_name: Corujo-Simon
- first_name: Christopher K.
full_name: Revell, Christopher K.
last_name: Revell
- first_name: Preeti
full_name: Sahu, Preeti
id: 55BA52EE-A185-11EA-88FD-18AD3DDC885E
last_name: Sahu
- first_name: Giuliano G.
full_name: Stirparo, Giuliano G.
last_name: Stirparo
- first_name: Irene M.
full_name: Aspalter, Irene M.
last_name: Aspalter
- first_name: Alex K.
full_name: Winkel, Alex K.
last_name: Winkel
- first_name: Ruby
full_name: Peters, Ruby
last_name: Peters
- first_name: Henry
full_name: De Belly, Henry
last_name: De Belly
- first_name: Davide A.D.
full_name: Cassani, Davide A.D.
last_name: Cassani
- first_name: Sarra
full_name: Achouri, Sarra
last_name: Achouri
- first_name: Raphael
full_name: Blumenfeld, Raphael
last_name: Blumenfeld
- first_name: Kristian
full_name: Franze, Kristian
last_name: Franze
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Ewa K.
full_name: Paluch, Ewa K.
last_name: Paluch
- first_name: Jennifer
full_name: Nichols, Jennifer
last_name: Nichols
- first_name: Kevin J.
full_name: Chalut, Kevin J.
last_name: Chalut
citation:
ama: Yanagida A, Corujo-Simon E, Revell CK, et al. Cell surface fluctuations regulate
early embryonic lineage sorting. Cell. 2022;185(5):777-793.e20. doi:10.1016/j.cell.2022.01.022
apa: Yanagida, A., Corujo-Simon, E., Revell, C. K., Sahu, P., Stirparo, G. G., Aspalter,
I. M., … Chalut, K. J. (2022). Cell surface fluctuations regulate early embryonic
lineage sorting. Cell. Cell Press. https://doi.org/10.1016/j.cell.2022.01.022
chicago: Yanagida, Ayaka, Elena Corujo-Simon, Christopher K. Revell, Preeti Sahu,
Giuliano G. Stirparo, Irene M. Aspalter, Alex K. Winkel, et al. “Cell Surface
Fluctuations Regulate Early Embryonic Lineage Sorting.” Cell. Cell Press,
2022. https://doi.org/10.1016/j.cell.2022.01.022.
ieee: A. Yanagida et al., “Cell surface fluctuations regulate early embryonic
lineage sorting,” Cell, vol. 185, no. 5. Cell Press, p. 777–793.e20, 2022.
ista: Yanagida A, Corujo-Simon E, Revell CK, Sahu P, Stirparo GG, Aspalter IM, Winkel
AK, Peters R, De Belly H, Cassani DAD, Achouri S, Blumenfeld R, Franze K, Hannezo
EB, Paluch EK, Nichols J, Chalut KJ. 2022. Cell surface fluctuations regulate
early embryonic lineage sorting. Cell. 185(5), 777–793.e20.
mla: Yanagida, Ayaka, et al. “Cell Surface Fluctuations Regulate Early Embryonic
Lineage Sorting.” Cell, vol. 185, no. 5, Cell Press, 2022, p. 777–793.e20,
doi:10.1016/j.cell.2022.01.022.
short: A. Yanagida, E. Corujo-Simon, C.K. Revell, P. Sahu, G.G. Stirparo, I.M. Aspalter,
A.K. Winkel, R. Peters, H. De Belly, D.A.D. Cassani, S. Achouri, R. Blumenfeld,
K. Franze, E.B. Hannezo, E.K. Paluch, J. Nichols, K.J. Chalut, Cell 185 (2022)
777–793.e20.
date_created: 2022-03-06T23:01:52Z
date_published: 2022-02-22T00:00:00Z
date_updated: 2023-08-02T14:43:50Z
day: '22'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1016/j.cell.2022.01.022
ec_funded: 1
external_id:
isi:
- '000796293700007'
pmid:
- '35196500'
file:
- access_level: open_access
checksum: ae305060e8031297771b89dae9e36a29
content_type: application/pdf
creator: dernst
date_created: 2022-03-07T07:55:23Z
date_updated: 2022-03-07T07:55:23Z
file_id: '10831'
file_name: 2022_Cell_Yanagida.pdf
file_size: 8478995
relation: main_file
success: 1
file_date_updated: 2022-03-07T07:55:23Z
has_accepted_license: '1'
intvolume: ' 185'
isi: 1
issue: '5'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 777-793.e20
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Cell Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cell surface fluctuations regulate early embryonic lineage sorting
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 185
year: '2022'
...
---
_id: '9316'
abstract:
- lang: eng
text: Embryo morphogenesis is impacted by dynamic changes in tissue material properties,
which have been proposed to occur via processes akin to phase transitions (PTs).
Here, we show that rigidity percolation provides a simple and robust theoretical
framework to predict material/structural PTs of embryonic tissues from local cell
connectivity. By using percolation theory, combined with directly monitoring dynamic
changes in tissue rheology and cell contact mechanics, we demonstrate that the
zebrafish blastoderm undergoes a genuine rigidity PT, brought about by a small
reduction in adhesion-dependent cell connectivity below a critical value. We quantitatively
predict and experimentally verify hallmarks of PTs, including power-law exponents
and associated discontinuities of macroscopic observables. Finally, we show that
this uniform PT depends on blastoderm cells undergoing meta-synchronous divisions
causing random and, consequently, uniform changes in cell connectivity. Collectively,
our theoretical and experimental findings reveal the structural basis of material
PTs in an organismal context.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank Carl Goodrich and the members of the Heisenberg and Hannezo
groups, in particular Reka Korei, for help, technical advice, and discussions; and
the Bioimaging and zebrafish facilities of the IST Austria for continuous support.
This work was supported by the Elise Richter Program of Austrian Science Fund (FWF)
to N.I.P. ( V 736-B26 ) and the European Union (European Research Council Advanced
Grant 742573 to C.-P.H. and European Research Council Starting Grant 851288 to E.H.).
article_processing_charge: No
article_type: original
author:
- first_name: Nicoletta
full_name: Petridou, Nicoletta
id: 2A003F6C-F248-11E8-B48F-1D18A9856A87
last_name: Petridou
orcid: 0000-0002-8451-1195
- first_name: Bernat
full_name: Corominas-Murtra, Bernat
id: 43BE2298-F248-11E8-B48F-1D18A9856A87
last_name: Corominas-Murtra
orcid: 0000-0001-9806-5643
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
citation:
ama: Petridou N, Corominas-Murtra B, Heisenberg C-PJ, Hannezo EB. Rigidity percolation
uncovers a structural basis for embryonic tissue phase transitions. Cell.
2021;184(7):1914-1928.e19. doi:10.1016/j.cell.2021.02.017
apa: Petridou, N., Corominas-Murtra, B., Heisenberg, C.-P. J., & Hannezo, E.
B. (2021). Rigidity percolation uncovers a structural basis for embryonic tissue
phase transitions. Cell. Elsevier. https://doi.org/10.1016/j.cell.2021.02.017
chicago: Petridou, Nicoletta, Bernat Corominas-Murtra, Carl-Philipp J Heisenberg,
and Edouard B Hannezo. “Rigidity Percolation Uncovers a Structural Basis for Embryonic
Tissue Phase Transitions.” Cell. Elsevier, 2021. https://doi.org/10.1016/j.cell.2021.02.017.
ieee: N. Petridou, B. Corominas-Murtra, C.-P. J. Heisenberg, and E. B. Hannezo,
“Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions,”
Cell, vol. 184, no. 7. Elsevier, p. 1914–1928.e19, 2021.
ista: Petridou N, Corominas-Murtra B, Heisenberg C-PJ, Hannezo EB. 2021. Rigidity
percolation uncovers a structural basis for embryonic tissue phase transitions.
Cell. 184(7), 1914–1928.e19.
mla: Petridou, Nicoletta, et al. “Rigidity Percolation Uncovers a Structural Basis
for Embryonic Tissue Phase Transitions.” Cell, vol. 184, no. 7, Elsevier,
2021, p. 1914–1928.e19, doi:10.1016/j.cell.2021.02.017.
short: N. Petridou, B. Corominas-Murtra, C.-P.J. Heisenberg, E.B. Hannezo, Cell
184 (2021) 1914–1928.e19.
date_created: 2021-04-11T22:01:14Z
date_published: 2021-04-01T00:00:00Z
date_updated: 2023-08-07T14:33:59Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
doi: 10.1016/j.cell.2021.02.017
ec_funded: 1
external_id:
isi:
- '000636734000022'
pmid:
- '33730596'
file:
- access_level: open_access
checksum: 1e5295fbd9c2a459173ec45a0e8a7c2e
content_type: application/pdf
creator: cziletti
date_created: 2021-06-08T10:04:10Z
date_updated: 2021-06-08T10:04:10Z
file_id: '9534'
file_name: 2021_Cell_Petridou.pdf
file_size: 11405875
relation: main_file
success: 1
file_date_updated: 2021-06-08T10:04:10Z
has_accepted_license: '1'
intvolume: ' 184'
isi: 1
issue: '7'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 1914-1928.e19
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
- _id: 2693FD8C-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: V00736
name: Tissue material properties in embryonic development
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/embryonic-tissue-undergoes-phase-transition/
scopus_import: '1'
status: public
title: Rigidity percolation uncovers a structural basis for embryonic tissue phase
transitions
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 184
year: '2021'
...
---
_id: '7789'
abstract:
- lang: eng
text: During embryonic and postnatal development, organs and tissues grow steadily
to achieve their final size at the end of puberty. However, little is known about
the cellular dynamics that mediate postnatal growth. By combining in vivo clonal
lineage tracing, proliferation kinetics, single-cell transcriptomics, andin vitro
micro-pattern experiments, we resolved the cellular dynamics taking place during
postnatal skin epidermis expansion. Our data revealed that harmonious growth is
engineered by a single population of developmental progenitors presenting a fixed
fate imbalance of self-renewing divisions with an ever-decreasing proliferation
rate. Single-cell RNA sequencing revealed that epidermal developmental progenitors
form a more uniform population compared with adult stem and progenitor cells.
Finally, we found that the spatial pattern of cell division orientation is dictated
locally by the underlying collagen fiber orientation. Our results uncover a simple
design principle of organ growth where progenitors and differentiated cells expand
in harmony with their surrounding tissues.
article_processing_charge: No
article_type: original
author:
- first_name: Sophie
full_name: Dekoninck, Sophie
last_name: Dekoninck
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Alejandro
full_name: Sifrim, Alejandro
last_name: Sifrim
- first_name: Yekaterina A.
full_name: Miroshnikova, Yekaterina A.
last_name: Miroshnikova
- first_name: Mariaceleste
full_name: Aragona, Mariaceleste
last_name: Aragona
- first_name: Milan
full_name: Malfait, Milan
last_name: Malfait
- first_name: Souhir
full_name: Gargouri, Souhir
last_name: Gargouri
- first_name: Charlotte
full_name: De Neunheuser, Charlotte
last_name: De Neunheuser
- first_name: Christine
full_name: Dubois, Christine
last_name: Dubois
- first_name: Thierry
full_name: Voet, Thierry
last_name: Voet
- first_name: Sara A.
full_name: Wickström, Sara A.
last_name: Wickström
- first_name: Benjamin D.
full_name: Simons, Benjamin D.
last_name: Simons
- first_name: Cédric
full_name: Blanpain, Cédric
last_name: Blanpain
citation:
ama: Dekoninck S, Hannezo EB, Sifrim A, et al. Defining the design principles of
skin epidermis postnatal growth. Cell. 2020;181(3):604-620.e22. doi:10.1016/j.cell.2020.03.015
apa: Dekoninck, S., Hannezo, E. B., Sifrim, A., Miroshnikova, Y. A., Aragona, M.,
Malfait, M., … Blanpain, C. (2020). Defining the design principles of skin epidermis
postnatal growth. Cell. Elsevier. https://doi.org/10.1016/j.cell.2020.03.015
chicago: Dekoninck, Sophie, Edouard B Hannezo, Alejandro Sifrim, Yekaterina A. Miroshnikova,
Mariaceleste Aragona, Milan Malfait, Souhir Gargouri, et al. “Defining the Design
Principles of Skin Epidermis Postnatal Growth.” Cell. Elsevier, 2020. https://doi.org/10.1016/j.cell.2020.03.015.
ieee: S. Dekoninck et al., “Defining the design principles of skin epidermis
postnatal growth,” Cell, vol. 181, no. 3. Elsevier, p. 604–620.e22, 2020.
ista: Dekoninck S, Hannezo EB, Sifrim A, Miroshnikova YA, Aragona M, Malfait M,
Gargouri S, De Neunheuser C, Dubois C, Voet T, Wickström SA, Simons BD, Blanpain
C. 2020. Defining the design principles of skin epidermis postnatal growth. Cell.
181(3), 604–620.e22.
mla: Dekoninck, Sophie, et al. “Defining the Design Principles of Skin Epidermis
Postnatal Growth.” Cell, vol. 181, no. 3, Elsevier, 2020, p. 604–620.e22,
doi:10.1016/j.cell.2020.03.015.
short: S. Dekoninck, E.B. Hannezo, A. Sifrim, Y.A. Miroshnikova, M. Aragona, M.
Malfait, S. Gargouri, C. De Neunheuser, C. Dubois, T. Voet, S.A. Wickström, B.D.
Simons, C. Blanpain, Cell 181 (2020) 604–620.e22.
date_created: 2020-05-03T22:00:48Z
date_published: 2020-04-30T00:00:00Z
date_updated: 2023-08-21T06:17:43Z
day: '30'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1016/j.cell.2020.03.015
external_id:
isi:
- '000530708400016'
pmid:
- '32259486'
file:
- access_level: open_access
checksum: e2114902f4e9d75a752e9efb5ae06011
content_type: application/pdf
creator: dernst
date_created: 2020-05-04T10:20:55Z
date_updated: 2020-07-14T12:48:03Z
file_id: '7795'
file_name: 2020_Cell_Dekoninck.pdf
file_size: 17992888
relation: main_file
file_date_updated: 2020-07-14T12:48:03Z
has_accepted_license: '1'
intvolume: ' 181'
isi: 1
issue: '3'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 604-620.e22
pmid: 1
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Defining the design principles of skin epidermis postnatal growth
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 181
year: '2020'
...
---
_id: '6351'
abstract:
- lang: eng
text: "A process of restorative patterning in plant roots correctly replaces eliminated
cells to heal local injuries despite the absence of cell migration, which underpins
wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell
divisions and acquisition of specific cell identities. Plants regularly endure
wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary
abilities to restore their tissues after injuries. Here, we provide insight into
a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted
elimination of different cells in Arabidopsis root combined with live-imaging
tracking during vertical growth allowed analysis of the regeneration processes
in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated
their stem cell transcriptional programs. They accelerated their progression through
cell cycle, coordinately changed the cell division orientation, and ultimately
acquired de novo the correct cell fates to replace missing cells. These observations
highlight existence of unknown intercellular positional signaling and demonstrate
the capability of specified cells to re-acquire stem cell programs as a crucial
part of the plant-specific mechanism of wound healing."
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
author:
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell.
2019;177(4):957-969.e13. doi:10.1016/j.cell.2019.04.015
apa: Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., & Friml,
J. (2019). Re-activation of stem cell pathways for pattern restoration in plant
wound healing. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.015
chicago: Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková,
and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in
Plant Wound Healing.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.015.
ieee: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml,
“Re-activation of stem cell pathways for pattern restoration in plant wound healing,”
Cell, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019.
ista: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4),
957–969.e13.
mla: Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration
in Plant Wound Healing.” Cell, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13,
doi:10.1016/j.cell.2019.04.015.
short: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell
177 (2019) 957–969.e13.
date_created: 2019-04-28T21:59:14Z
date_published: 2019-05-02T00:00:00Z
date_updated: 2024-03-25T23:30:06Z
day: '02'
ddc:
- '570'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cell.2019.04.015
ec_funded: 1
external_id:
isi:
- '000466843000015'
pmid:
- '31051107'
file:
- access_level: open_access
checksum: 4ceba04a96a74f5092ec3ce2c579a0c7
content_type: application/pdf
creator: dernst
date_created: 2019-05-13T06:12:45Z
date_updated: 2020-07-14T12:47:28Z
file_id: '6411'
file_name: 2019_Cell_Marhava.pdf
file_size: 10272032
relation: main_file
file_date_updated: 2020-07-14T12:47:28Z
has_accepted_license: '1'
intvolume: ' 177'
isi: 1
issue: '4'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 957-969.e13
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/specialized-plant-cells-regain-stem-cell-features-to-heal-wounds/
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Re-activation of stem cell pathways for pattern restoration in plant wound
healing
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 177
year: '2019'
...
---
_id: '6508'
abstract:
- lang: eng
text: Segregation of maternal determinants within the oocyte constitutes the first
step in embryo patterning. In zebrafish oocytes, extensive ooplasmic streaming
leads to the segregation of ooplasm from yolk granules along the animal-vegetal
axis of the oocyte. Here, we show that this process does not rely on cortical
actin reorganization, as previously thought, but instead on a cell-cycle-dependent
bulk actin polymerization wave traveling from the animal to the vegetal pole of
the oocyte. This wave functions in segregation by both pulling ooplasm animally
and pushing yolk granules vegetally. Using biophysical experimentation and theory,
we show that ooplasm pulling is mediated by bulk actin network flows exerting
friction forces on the ooplasm, while yolk granule pushing is achieved by a mechanism
closely resembling actin comet formation on yolk granules. Our study defines a
novel role of cell-cycle-controlled bulk actin polymerization waves in oocyte
polarization via ooplasmic segregation.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We would like to thank Pierre Recho, Guillaume Salbreux, and Silvia
Grigolon for advice on the theory, Lila Solnica-Krezel for kindly providing us with
zebrafish dachsous mutants, members of the Heisenberg and Hannezo groups for fruitful
discussions, and the Bioimaging and zebrafish facilities at IST Austria for their
continuous support. This project has received funding from the European Union (European
Research Council Advanced Grant 742573 to C.P.H.) and from the Austrian Science
Fund (FWF) (P 31639 to E.H.).
article_processing_charge: No
article_type: original
author:
- first_name: Shayan
full_name: Shamipour, Shayan
id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
last_name: Shamipour
- first_name: Roland
full_name: Kardos, Roland
id: 4039350E-F248-11E8-B48F-1D18A9856A87
last_name: Kardos
- first_name: Shi-lei
full_name: Xue, Shi-lei
id: 31D2C804-F248-11E8-B48F-1D18A9856A87
last_name: Xue
- first_name: Björn
full_name: Hof, Björn
id: 3A374330-F248-11E8-B48F-1D18A9856A87
last_name: Hof
orcid: 0000-0003-2057-2754
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. Bulk actin
dynamics drive phase segregation in zebrafish oocytes. Cell. 2019;177(6):1463-1479.e18.
doi:10.1016/j.cell.2019.04.030
apa: Shamipour, S., Kardos, R., Xue, S., Hof, B., Hannezo, E. B., & Heisenberg,
C.-P. J. (2019). Bulk actin dynamics drive phase segregation in zebrafish oocytes.
Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.030
chicago: Shamipour, Shayan, Roland Kardos, Shi-lei Xue, Björn Hof, Edouard B Hannezo,
and Carl-Philipp J Heisenberg. “Bulk Actin Dynamics Drive Phase Segregation in
Zebrafish Oocytes.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.030.
ieee: S. Shamipour, R. Kardos, S. Xue, B. Hof, E. B. Hannezo, and C.-P. J. Heisenberg,
“Bulk actin dynamics drive phase segregation in zebrafish oocytes,” Cell,
vol. 177, no. 6. Elsevier, p. 1463–1479.e18, 2019.
ista: Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. 2019. Bulk
actin dynamics drive phase segregation in zebrafish oocytes. Cell. 177(6), 1463–1479.e18.
mla: Shamipour, Shayan, et al. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish
Oocytes.” Cell, vol. 177, no. 6, Elsevier, 2019, p. 1463–1479.e18, doi:10.1016/j.cell.2019.04.030.
short: S. Shamipour, R. Kardos, S. Xue, B. Hof, E.B. Hannezo, C.-P.J. Heisenberg,
Cell 177 (2019) 1463–1479.e18.
date_created: 2019-06-02T21:59:12Z
date_published: 2019-05-30T00:00:00Z
date_updated: 2024-03-25T23:30:21Z
day: '30'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
- _id: BjHo
doi: 10.1016/j.cell.2019.04.030
ec_funded: 1
external_id:
isi:
- '000469415100013'
pmid:
- '31080065'
file:
- access_level: open_access
checksum: aea43726d80e35ce3885073a5f05c3e3
content_type: application/pdf
creator: dernst
date_created: 2020-10-21T07:22:34Z
date_updated: 2020-10-21T07:22:34Z
file_id: '8686'
file_name: 2019_Cell_Shamipour_accepted.pdf
file_size: 3356292
relation: main_file
success: 1
file_date_updated: 2020-10-21T07:22:34Z
has_accepted_license: '1'
intvolume: ' 177'
isi: 1
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1016/j.cell.2019.04.030
month: '05'
oa: 1
oa_version: Published Version
page: 1463-1479.e18
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
- _id: 268294B6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P31639
name: Active mechano-chemical description of the cell cytoskeleton
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/how-the-cytoplasm-separates-from-the-yolk/
record:
- id: '8350'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Bulk actin dynamics drive phase segregation in zebrafish oocytes
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 177
year: '2019'
...
---
_id: '6601'
abstract:
- lang: eng
text: There is increasing evidence that both mechanical and biochemical signals
play important roles in development and disease. The development of complex organisms,
in particular, has been proposed to rely on the feedback between mechanical and
biochemical patterning events. This feedback occurs at the molecular level via
mechanosensation but can also arise as an emergent property of the system at the
cellular and tissue level. In recent years, dynamic changes in tissue geometry,
flow, rheology, and cell fate specification have emerged as key platforms of mechanochemical
feedback loops in multiple processes. Here, we review recent experimental and
theoretical advances in understanding how these feedbacks function in development
and disease.
article_processing_charge: No
article_type: review
author:
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Hannezo EB, Heisenberg C-PJ. Mechanochemical feedback loops in development
and disease. Cell. 2019;178(1):12-25. doi:10.1016/j.cell.2019.05.052
apa: Hannezo, E. B., & Heisenberg, C.-P. J. (2019). Mechanochemical feedback
loops in development and disease. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.05.052
chicago: Hannezo, Edouard B, and Carl-Philipp J Heisenberg. “Mechanochemical Feedback
Loops in Development and Disease.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.05.052.
ieee: E. B. Hannezo and C.-P. J. Heisenberg, “Mechanochemical feedback loops in
development and disease,” Cell, vol. 178, no. 1. Elsevier, pp. 12–25, 2019.
ista: Hannezo EB, Heisenberg C-PJ. 2019. Mechanochemical feedback loops in development
and disease. Cell. 178(1), 12–25.
mla: Hannezo, Edouard B., and Carl-Philipp J. Heisenberg. “Mechanochemical Feedback
Loops in Development and Disease.” Cell, vol. 178, no. 1, Elsevier, 2019,
pp. 12–25, doi:10.1016/j.cell.2019.05.052.
short: E.B. Hannezo, C.-P.J. Heisenberg, Cell 178 (2019) 12–25.
date_created: 2019-06-30T21:59:11Z
date_published: 2019-07-27T00:00:00Z
date_updated: 2023-08-28T12:25:21Z
day: '27'
department:
- _id: CaHe
- _id: EdHa
doi: 10.1016/j.cell.2019.05.052
ec_funded: 1
external_id:
isi:
- '000473002700005'
pmid:
- '31251912'
intvolume: ' 178'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1016/j.cell.2019.05.052
month: '07'
oa: 1
oa_version: Published Version
page: 12-25
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
- _id: 268294B6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P31639
name: Active mechano-chemical description of the cell cytoskeleton
publication: Cell
publication_identifier:
issn:
- '00928674'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanochemical feedback loops in development and disease
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 178
year: '2019'
...
---
_id: '803'
abstract:
- lang: eng
text: Eukaryotic cells store their chromosomes in a single nucleus. This is important
to maintain genomic integrity, as chromosomes packaged into separate nuclei (micronuclei)
are prone to massive DNA damage. During mitosis, higher eukaryotes disassemble
their nucleus and release individualized chromosomes for segregation. How numerous
chromosomes subsequently reform a single nucleus has remained unclear. Using image-based
screening of human cells, we identified barrier-to-autointegration factor (BAF)
as a key factor guiding membranes to form a single nucleus. Unexpectedly, nuclear
assembly does not require BAF?s association with inner nuclear membrane proteins
but instead relies on BAF?s ability to bridge distant DNA sites. Live-cell imaging
and in vitro reconstitution showed that BAF enriches around the mitotic chromosome
ensemble to induce a densely cross-bridged chromatin layer that is mechanically
stiff and limits membranes to the surface. Our study reveals that BAF-mediated
changes in chromosome mechanics underlie nuclear assembly with broad implications
for proper genome function.
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
author:
- first_name: Matthias
full_name: Samwer, Matthias
last_name: Samwer
- first_name: Maximilian
full_name: Schneider, Maximilian
last_name: Schneider
- first_name: Rudolf
full_name: Hoefler, Rudolf
last_name: Hoefler
- first_name: Philipp S
full_name: Schmalhorst, Philipp S
id: 309D50DA-F248-11E8-B48F-1D18A9856A87
last_name: Schmalhorst
orcid: 0000-0002-5795-0133
- first_name: Julian
full_name: Jude, Julian
last_name: Jude
- first_name: Johannes
full_name: Zuber, Johannes
last_name: Zuber
- first_name: Daniel
full_name: Gerlic, Daniel
last_name: Gerlic
citation:
ama: Samwer M, Schneider M, Hoefler R, et al. DNA cross-bridging shapes a single
nucleus from a set of mitotic chromosomes. Cell. 2017;170(5):956-972. doi:10.1016/j.cell.2017.07.038
apa: Samwer, M., Schneider, M., Hoefler, R., Schmalhorst, P. S., Jude, J., Zuber,
J., & Gerlic, D. (2017). DNA cross-bridging shapes a single nucleus from a
set of mitotic chromosomes. Cell. Cell Press. https://doi.org/10.1016/j.cell.2017.07.038
chicago: Samwer, Matthias, Maximilian Schneider, Rudolf Hoefler, Philipp S Schmalhorst,
Julian Jude, Johannes Zuber, and Daniel Gerlic. “DNA Cross-Bridging Shapes a Single
Nucleus from a Set of Mitotic Chromosomes.” Cell. Cell Press, 2017. https://doi.org/10.1016/j.cell.2017.07.038.
ieee: M. Samwer et al., “DNA cross-bridging shapes a single nucleus from
a set of mitotic chromosomes,” Cell, vol. 170, no. 5. Cell Press, pp. 956–972,
2017.
ista: Samwer M, Schneider M, Hoefler R, Schmalhorst PS, Jude J, Zuber J, Gerlic
D. 2017. DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes.
Cell. 170(5), 956–972.
mla: Samwer, Matthias, et al. “DNA Cross-Bridging Shapes a Single Nucleus from a
Set of Mitotic Chromosomes.” Cell, vol. 170, no. 5, Cell Press, 2017, pp.
956–72, doi:10.1016/j.cell.2017.07.038.
short: M. Samwer, M. Schneider, R. Hoefler, P.S. Schmalhorst, J. Jude, J. Zuber,
D. Gerlic, Cell 170 (2017) 956–972.
date_created: 2018-12-11T11:48:35Z
date_published: 2017-08-24T00:00:00Z
date_updated: 2023-09-27T10:59:14Z
day: '24'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1016/j.cell.2017.07.038
external_id:
isi:
- '000408372400014'
file:
- access_level: open_access
checksum: 64897b0c5373f22273f598e4672c60ff
content_type: application/pdf
creator: dernst
date_created: 2019-01-18T13:45:40Z
date_updated: 2020-07-14T12:48:08Z
file_id: '5852'
file_name: 2017_Cell_Samwer.pdf
file_size: 17666637
relation: main_file
file_date_updated: 2020-07-14T12:48:08Z
has_accepted_license: '1'
intvolume: ' 170'
isi: 1
issue: '5'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 956 - 972
publication: Cell
publication_identifier:
issn:
- '00928674'
publication_status: published
publisher: Cell Press
publist_id: '6848'
quality_controlled: '1'
scopus_import: '1'
status: public
title: DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 170
year: '2017'
...
---
_id: '726'
abstract:
- lang: eng
text: The morphogenesis of branched organs remains a subject of abiding interest.
Although much is known about the underlying signaling pathways, it remains unclear
how macroscopic features of branched organs, including their size, network topology,
and spatial patterning, are encoded. Here, we show that, in mouse mammary gland,
kidney, and human prostate, these features can be explained quantitatively within
a single unifying framework of branching and annihilating random walks. Based
on quantitative analyses of large-scale organ reconstructions and proliferation
kinetics measurements, we propose that morphogenesis follows from the proliferative
activity of equipotent tips that stochastically branch and randomly explore their
environment but compete neutrally for space, becoming proliferatively inactive
when in proximity with neighboring ducts. These results show that complex branched
epithelial structures develop as a self-organized process, reliant upon a strikingly
simple but generic rule, without recourse to a rigid and deterministic sequence
of genetically programmed events.
article_processing_charge: No
author:
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Colinda
full_name: Scheele, Colinda
last_name: Scheele
- first_name: Mohammad
full_name: Moad, Mohammad
last_name: Moad
- first_name: Nicholas
full_name: Drogo, Nicholas
last_name: Drogo
- first_name: Rakesh
full_name: Heer, Rakesh
last_name: Heer
- first_name: Rosemary
full_name: Sampogna, Rosemary
last_name: Sampogna
- first_name: Jacco
full_name: Van Rheenen, Jacco
last_name: Van Rheenen
- first_name: Benjamin
full_name: Simons, Benjamin
last_name: Simons
citation:
ama: Hannezo EB, Scheele C, Moad M, et al. A unifying theory of branching morphogenesis.
Cell. 2017;171(1):242-255. doi:10.1016/j.cell.2017.08.026
apa: Hannezo, E. B., Scheele, C., Moad, M., Drogo, N., Heer, R., Sampogna, R., …
Simons, B. (2017). A unifying theory of branching morphogenesis. Cell.
Cell Press. https://doi.org/10.1016/j.cell.2017.08.026
chicago: Hannezo, Edouard B, Colinda Scheele, Mohammad Moad, Nicholas Drogo, Rakesh
Heer, Rosemary Sampogna, Jacco Van Rheenen, and Benjamin Simons. “A Unifying Theory
of Branching Morphogenesis.” Cell. Cell Press, 2017. https://doi.org/10.1016/j.cell.2017.08.026.
ieee: E. B. Hannezo et al., “A unifying theory of branching morphogenesis,”
Cell, vol. 171, no. 1. Cell Press, pp. 242–255, 2017.
ista: Hannezo EB, Scheele C, Moad M, Drogo N, Heer R, Sampogna R, Van Rheenen J,
Simons B. 2017. A unifying theory of branching morphogenesis. Cell. 171(1), 242–255.
mla: Hannezo, Edouard B., et al. “A Unifying Theory of Branching Morphogenesis.”
Cell, vol. 171, no. 1, Cell Press, 2017, pp. 242–55, doi:10.1016/j.cell.2017.08.026.
short: E.B. Hannezo, C. Scheele, M. Moad, N. Drogo, R. Heer, R. Sampogna, J. Van
Rheenen, B. Simons, Cell 171 (2017) 242–255.
date_created: 2018-12-11T11:48:10Z
date_published: 2017-09-21T00:00:00Z
date_updated: 2023-09-28T11:34:17Z
day: '21'
ddc:
- '539'
department:
- _id: EdHa
doi: 10.1016/j.cell.2017.08.026
external_id:
isi:
- '000411331800024'
file:
- access_level: open_access
checksum: 7a036d93a9e2e597af9bb504d6133aca
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:17Z
date_updated: 2020-07-14T12:47:55Z
file_id: '4870'
file_name: IST-2017-883-v1+1_PIIS0092867417309510.pdf
file_size: 12670204
relation: main_file
file_date_updated: 2020-07-14T12:47:55Z
has_accepted_license: '1'
intvolume: ' 171'
isi: 1
issue: '1'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 242 - 255
publication: Cell
publication_identifier:
issn:
- '00928674'
publication_status: published
publisher: Cell Press
publist_id: '6952'
pubrep_id: '883'
quality_controlled: '1'
scopus_import: '1'
status: public
title: A unifying theory of branching morphogenesis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 171
year: '2017'
...
---
_id: '727'
abstract:
- lang: eng
text: 'Actin filaments polymerizing against membranes power endocytosis, vesicular
traffic, and cell motility. In vitro reconstitution studies suggest that the structure
and the dynamics of actin networks respond to mechanical forces. We demonstrate
that lamellipodial actin of migrating cells responds to mechanical load when membrane
tension is modulated. In a steady state, migrating cell filaments assume the canonical
dendritic geometry, defined by Arp2/3-generated 70° branch points. Increased tension
triggers a dense network with a broadened range of angles, whereas decreased tension
causes a shift to a sparse configuration dominated by filaments growing perpendicularly
to the plasma membrane. We show that these responses emerge from the geometry
of branched actin: when load per filament decreases, elongation speed increases
and perpendicular filaments gradually outcompete others because they polymerize
the shortest distance to the membrane, where they are protected from capping.
This network-intrinsic geometrical adaptation mechanism tunes protrusive force
in response to mechanical load.'
acknowledged_ssus:
- _id: ScienComp
article_processing_charge: No
author:
- first_name: Jan
full_name: Mueller, Jan
last_name: Mueller
- first_name: Gregory
full_name: Szep, Gregory
id: 4BFB7762-F248-11E8-B48F-1D18A9856A87
last_name: Szep
- first_name: Maria
full_name: Nemethova, Maria
id: 34E27F1C-F248-11E8-B48F-1D18A9856A87
last_name: Nemethova
- first_name: Ingrid
full_name: De Vries, Ingrid
id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
last_name: De Vries
- first_name: Arnon
full_name: Lieber, Arnon
last_name: Lieber
- first_name: Christoph
full_name: Winkler, Christoph
last_name: Winkler
- first_name: Karsten
full_name: Kruse, Karsten
last_name: Kruse
- first_name: John
full_name: Small, John
last_name: Small
- first_name: Christian
full_name: Schmeiser, Christian
last_name: Schmeiser
- first_name: Kinneret
full_name: Keren, Kinneret
last_name: Keren
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Mueller J, Szep G, Nemethova M, et al. Load adaptation of lamellipodial actin
networks. Cell. 2017;171(1):188-200. doi:10.1016/j.cell.2017.07.051
apa: Mueller, J., Szep, G., Nemethova, M., de Vries, I., Lieber, A., Winkler, C.,
… Sixt, M. K. (2017). Load adaptation of lamellipodial actin networks. Cell.
Cell Press. https://doi.org/10.1016/j.cell.2017.07.051
chicago: Mueller, Jan, Gregory Szep, Maria Nemethova, Ingrid de Vries, Arnon Lieber,
Christoph Winkler, Karsten Kruse, et al. “Load Adaptation of Lamellipodial Actin
Networks.” Cell. Cell Press, 2017. https://doi.org/10.1016/j.cell.2017.07.051.
ieee: J. Mueller et al., “Load adaptation of lamellipodial actin networks,”
Cell, vol. 171, no. 1. Cell Press, pp. 188–200, 2017.
ista: Mueller J, Szep G, Nemethova M, de Vries I, Lieber A, Winkler C, Kruse K,
Small J, Schmeiser C, Keren K, Hauschild R, Sixt MK. 2017. Load adaptation of
lamellipodial actin networks. Cell. 171(1), 188–200.
mla: Mueller, Jan, et al. “Load Adaptation of Lamellipodial Actin Networks.” Cell,
vol. 171, no. 1, Cell Press, 2017, pp. 188–200, doi:10.1016/j.cell.2017.07.051.
short: J. Mueller, G. Szep, M. Nemethova, I. de Vries, A. Lieber, C. Winkler, K.
Kruse, J. Small, C. Schmeiser, K. Keren, R. Hauschild, M.K. Sixt, Cell 171 (2017)
188–200.
date_created: 2018-12-11T11:48:10Z
date_published: 2017-09-21T00:00:00Z
date_updated: 2023-09-28T11:33:49Z
day: '21'
department:
- _id: MiSi
- _id: Bio
doi: 10.1016/j.cell.2017.07.051
ec_funded: 1
external_id:
isi:
- '000411331800020'
intvolume: ' 171'
isi: 1
issue: '1'
language:
- iso: eng
month: '09'
oa_version: None
page: 188 - 200
project:
- _id: 25AD6156-B435-11E9-9278-68D0E5697425
grant_number: LS13-029
name: Modeling of Polarization and Motility of Leukocytes in Three-Dimensional Environments
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '281556'
name: Cytoskeletal force generation and force transduction of migrating leukocytes
(EU)
publication: Cell
publication_identifier:
issn:
- '00928674'
publication_status: published
publisher: Cell Press
publist_id: '6951'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Load adaptation of lamellipodial actin networks
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 171
year: '2017'
...
---
_id: '571'
abstract:
- lang: eng
text: Blood platelets are critical for hemostasis and thrombosis and play diverse
roles during immune responses. Despite these versatile tasks in mammalian biology,
their skills on a cellular level are deemed limited, mainly consisting in rolling,
adhesion, and aggregate formation. Here, we identify an unappreciated asset of
platelets and show that adherent platelets use adhesion receptors to mechanically
probe the adhesive substrate in their local microenvironment. When actomyosin-dependent
traction forces overcome substrate resistance, platelets migrate and pile up the
adhesive substrate together with any bound particulate material. They use this
ability to act as cellular scavengers, scanning the vascular surface for potential
invaders and collecting deposited bacteria. Microbe collection by migrating platelets
boosts the activity of professional phagocytes, exacerbating inflammatory tissue
injury in sepsis. This assigns platelets a central role in innate immune responses
and identifies them as potential targets to dampen inflammatory tissue damage
in clinical scenarios of severe systemic infection. In addition to their role
in thrombosis and hemostasis, platelets can also migrate to sites of infection
to help trap bacteria and clear the vascular surface.
author:
- first_name: Florian R
full_name: Gärtner, Florian R
id: 397A88EE-F248-11E8-B48F-1D18A9856A87
last_name: Gärtner
orcid: 0000-0001-6120-3723
- first_name: Zerkah
full_name: Ahmad, Zerkah
last_name: Ahmad
- first_name: Gerhild
full_name: Rosenberger, Gerhild
last_name: Rosenberger
- first_name: Shuxia
full_name: Fan, Shuxia
last_name: Fan
- first_name: Leo
full_name: Nicolai, Leo
last_name: Nicolai
- first_name: Benjamin
full_name: Busch, Benjamin
last_name: Busch
- first_name: Gökce
full_name: Yavuz, Gökce
last_name: Yavuz
- first_name: Manja
full_name: Luckner, Manja
last_name: Luckner
- first_name: Hellen
full_name: Ishikawa Ankerhold, Hellen
last_name: Ishikawa Ankerhold
- first_name: Roman
full_name: Hennel, Roman
last_name: Hennel
- first_name: Alexandre
full_name: Benechet, Alexandre
last_name: Benechet
- first_name: Michael
full_name: Lorenz, Michael
last_name: Lorenz
- first_name: Sue
full_name: Chandraratne, Sue
last_name: Chandraratne
- first_name: Irene
full_name: Schubert, Irene
last_name: Schubert
- first_name: Sebastian
full_name: Helmer, Sebastian
last_name: Helmer
- first_name: Bianca
full_name: Striednig, Bianca
last_name: Striednig
- first_name: Konstantin
full_name: Stark, Konstantin
last_name: Stark
- first_name: Marek
full_name: Janko, Marek
last_name: Janko
- first_name: Ralph
full_name: Böttcher, Ralph
last_name: Böttcher
- first_name: Admar
full_name: Verschoor, Admar
last_name: Verschoor
- first_name: Catherine
full_name: Leon, Catherine
last_name: Leon
- first_name: Christian
full_name: Gachet, Christian
last_name: Gachet
- first_name: Thomas
full_name: Gudermann, Thomas
last_name: Gudermann
- first_name: Michael
full_name: Mederos Y Schnitzler, Michael
last_name: Mederos Y Schnitzler
- first_name: Zachary
full_name: Pincus, Zachary
last_name: Pincus
- first_name: Matteo
full_name: Iannacone, Matteo
last_name: Iannacone
- first_name: Rainer
full_name: Haas, Rainer
last_name: Haas
- first_name: Gerhard
full_name: Wanner, Gerhard
last_name: Wanner
- first_name: Kirsten
full_name: Lauber, Kirsten
last_name: Lauber
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Steffen
full_name: Massberg, Steffen
last_name: Massberg
citation:
ama: Gärtner FR, Ahmad Z, Rosenberger G, et al. Migrating platelets are mechano
scavengers that collect and bundle bacteria. Cell Press. 2017;171(6):1368-1382.
doi:10.1016/j.cell.2017.11.001
apa: Gärtner, F. R., Ahmad, Z., Rosenberger, G., Fan, S., Nicolai, L., Busch, B.,
… Massberg, S. (2017). Migrating platelets are mechano scavengers that collect
and bundle bacteria. Cell Press. Cell Press. https://doi.org/10.1016/j.cell.2017.11.001
chicago: Gärtner, Florian R, Zerkah Ahmad, Gerhild Rosenberger, Shuxia Fan, Leo
Nicolai, Benjamin Busch, Gökce Yavuz, et al. “Migrating Platelets Are Mechano
Scavengers That Collect and Bundle Bacteria.” Cell Press. Cell Press, 2017.
https://doi.org/10.1016/j.cell.2017.11.001.
ieee: F. R. Gärtner et al., “Migrating platelets are mechano scavengers that
collect and bundle bacteria,” Cell Press, vol. 171, no. 6. Cell Press,
pp. 1368–1382, 2017.
ista: Gärtner FR, Ahmad Z, Rosenberger G, Fan S, Nicolai L, Busch B, Yavuz G, Luckner
M, Ishikawa Ankerhold H, Hennel R, Benechet A, Lorenz M, Chandraratne S, Schubert
I, Helmer S, Striednig B, Stark K, Janko M, Böttcher R, Verschoor A, Leon C, Gachet
C, Gudermann T, Mederos Y Schnitzler M, Pincus Z, Iannacone M, Haas R, Wanner
G, Lauber K, Sixt MK, Massberg S. 2017. Migrating platelets are mechano scavengers
that collect and bundle bacteria. Cell Press. 171(6), 1368–1382.
mla: Gärtner, Florian R., et al. “Migrating Platelets Are Mechano Scavengers That
Collect and Bundle Bacteria.” Cell Press, vol. 171, no. 6, Cell Press,
2017, pp. 1368–82, doi:10.1016/j.cell.2017.11.001.
short: F.R. Gärtner, Z. Ahmad, G. Rosenberger, S. Fan, L. Nicolai, B. Busch, G.
Yavuz, M. Luckner, H. Ishikawa Ankerhold, R. Hennel, A. Benechet, M. Lorenz, S.
Chandraratne, I. Schubert, S. Helmer, B. Striednig, K. Stark, M. Janko, R. Böttcher,
A. Verschoor, C. Leon, C. Gachet, T. Gudermann, M. Mederos Y Schnitzler, Z. Pincus,
M. Iannacone, R. Haas, G. Wanner, K. Lauber, M.K. Sixt, S. Massberg, Cell Press
171 (2017) 1368–1382.
date_created: 2018-12-11T11:47:15Z
date_published: 2017-11-30T00:00:00Z
date_updated: 2021-01-12T08:03:15Z
day: '30'
department:
- _id: MiSi
doi: 10.1016/j.cell.2017.11.001
ec_funded: 1
intvolume: ' 171'
issue: '6'
language:
- iso: eng
month: '11'
oa_version: None
page: 1368 - 1382
project:
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '747687'
name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
publication: Cell Press
publication_identifier:
issn:
- '00928674'
publication_status: published
publisher: Cell Press
publist_id: '7243'
quality_controlled: '1'
scopus_import: 1
status: public
title: Migrating platelets are mechano scavengers that collect and bundle bacteria
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 171
year: '2017'
...
---
_id: '600'
abstract:
- lang: eng
text: Transcription initiation at the ribosomal RNA promoter requires RNA polymerase
(Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine
X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular
model for basal Pol I initiation. The three-subunit CF binds upstream promoter
DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center
cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp
domains enables cleft contraction, resulting in an active Pol I conformation and
RNA synthesis. Comparison with the Pol II system suggests that promoter specificity
relies on a distinct “bendability” and “meltability” of the promoter sequence
that enables contacts between initiation factors, DNA, and polymerase.
article_processing_charge: No
author:
- first_name: Christoph
full_name: Engel, Christoph
last_name: Engel
- first_name: Tobias
full_name: Gubbey, Tobias
last_name: Gubbey
- first_name: Simon
full_name: Neyer, Simon
last_name: Neyer
- first_name: Sarah
full_name: Sainsbury, Sarah
last_name: Sainsbury
- first_name: Christiane
full_name: Oberthuer, Christiane
last_name: Oberthuer
- first_name: Carlo
full_name: Baejen, Carlo
last_name: Baejen
- first_name: Carrie A
full_name: Bernecky, Carrie A
id: 2CB9DFE2-F248-11E8-B48F-1D18A9856A87
last_name: Bernecky
orcid: 0000-0003-0893-7036
- first_name: Patrick
full_name: Cramer, Patrick
last_name: Cramer
citation:
ama: Engel C, Gubbey T, Neyer S, et al. Structural basis of RNA polymerase I transcription
initiation. Cell. 2017;169(1):120-131.e22. doi:10.1016/j.cell.2017.03.003
apa: Engel, C., Gubbey, T., Neyer, S., Sainsbury, S., Oberthuer, C., Baejen, C.,
… Cramer, P. (2017). Structural basis of RNA polymerase I transcription initiation.
Cell. Cell Press. https://doi.org/10.1016/j.cell.2017.03.003
chicago: Engel, Christoph, Tobias Gubbey, Simon Neyer, Sarah Sainsbury, Christiane
Oberthuer, Carlo Baejen, Carrie Bernecky, and Patrick Cramer. “Structural Basis
of RNA Polymerase I Transcription Initiation.” Cell. Cell Press, 2017.
https://doi.org/10.1016/j.cell.2017.03.003.
ieee: C. Engel et al., “Structural basis of RNA polymerase I transcription
initiation,” Cell, vol. 169, no. 1. Cell Press, p. 120–131.e22, 2017.
ista: Engel C, Gubbey T, Neyer S, Sainsbury S, Oberthuer C, Baejen C, Bernecky C,
Cramer P. 2017. Structural basis of RNA polymerase I transcription initiation.
Cell. 169(1), 120–131.e22.
mla: Engel, Christoph, et al. “Structural Basis of RNA Polymerase I Transcription
Initiation.” Cell, vol. 169, no. 1, Cell Press, 2017, p. 120–131.e22, doi:10.1016/j.cell.2017.03.003.
short: C. Engel, T. Gubbey, S. Neyer, S. Sainsbury, C. Oberthuer, C. Baejen, C.
Bernecky, P. Cramer, Cell 169 (2017) 120–131.e22.
date_created: 2018-12-11T11:47:25Z
date_published: 2017-03-23T00:00:00Z
date_updated: 2021-01-12T08:05:36Z
day: '23'
doi: 10.1016/j.cell.2017.03.003
extern: '1'
intvolume: ' 169'
issue: '1'
language:
- iso: eng
month: '03'
oa_version: None
page: 120 - 131.e22
publication: Cell
publication_identifier:
issn:
- '00928674'
publication_status: published
publisher: Cell Press
publist_id: '7204'
quality_controlled: '1'
status: public
title: Structural basis of RNA polymerase I transcription initiation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 169
year: '2017'
...
---
_id: '2240'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma
membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is
essential for physiology and development, but the identification and organization
of the machinery operating this process remains largely obscure. Here, we identified
an eight-core-component protein complex, the TPLATE complex, essential for plant
growth via its role as major adaptor module for clathrin-mediated endocytosis.
This complex consists of evolutionarily unique proteins that associate closely
with core endocytic elements. The TPLATE complex is recruited as dynamic foci
at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin,
and dynamin-related proteins. Reduced function of different complex components
severely impaired internalization of assorted endocytic cargoes, demonstrating
its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE
complex is an early endocytic module representing a unique evolutionary plant
adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.
author:
- first_name: Astrid
full_name: Gadeyne, Astrid
last_name: Gadeyne
- first_name: Clara
full_name: Sánchez Rodríguez, Clara
last_name: Sánchez Rodríguez
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Simone
full_name: Di Rubbo, Simone
last_name: Di Rubbo
- first_name: Henrik
full_name: Zauber, Henrik
last_name: Zauber
- first_name: Kevin
full_name: Vanneste, Kevin
last_name: Vanneste
- first_name: Jelle
full_name: Van Leene, Jelle
last_name: Van Leene
- first_name: Nancy
full_name: De Winne, Nancy
last_name: De Winne
- first_name: Dominique
full_name: Eeckhout, Dominique
last_name: Eeckhout
- first_name: Geert
full_name: Persiau, Geert
last_name: Persiau
- first_name: Eveline
full_name: Van De Slijke, Eveline
last_name: Van De Slijke
- first_name: Bernard
full_name: Cannoot, Bernard
last_name: Cannoot
- first_name: Leen
full_name: Vercruysse, Leen
last_name: Vercruysse
- first_name: Jonathan
full_name: Mayers, Jonathan
last_name: Mayers
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Matthias
full_name: Ehrlich, Matthias
last_name: Ehrlich
- first_name: Alois
full_name: Schweighofer, Alois
last_name: Schweighofer
- first_name: Tijs
full_name: Ketelaar, Tijs
last_name: Ketelaar
- first_name: Steven
full_name: Maere, Steven
last_name: Maere
- first_name: Sebastian
full_name: Bednarek, Sebastian
last_name: Bednarek
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Erwin
full_name: Witters, Erwin
last_name: Witters
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Staffan
full_name: Persson, Staffan
last_name: Persson
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Daniël
full_name: Van Damme, Daniël
last_name: Van Damme
citation:
ama: Gadeyne A, Sánchez Rodríguez C, Vanneste S, et al. The TPLATE adaptor complex
drives clathrin-mediated endocytosis in plants. Cell. 2014;156(4):691-704.
doi:10.1016/j.cell.2014.01.039
apa: Gadeyne, A., Sánchez Rodríguez, C., Vanneste, S., Di Rubbo, S., Zauber, H.,
Vanneste, K., … Van Damme, D. (2014). The TPLATE adaptor complex drives clathrin-mediated
endocytosis in plants. Cell. Cell Press. https://doi.org/10.1016/j.cell.2014.01.039
chicago: Gadeyne, Astrid, Clara Sánchez Rodríguez, Steffen Vanneste, Simone Di Rubbo,
Henrik Zauber, Kevin Vanneste, Jelle Van Leene, et al. “The TPLATE Adaptor Complex
Drives Clathrin-Mediated Endocytosis in Plants.” Cell. Cell Press, 2014.
https://doi.org/10.1016/j.cell.2014.01.039.
ieee: A. Gadeyne et al., “The TPLATE adaptor complex drives clathrin-mediated
endocytosis in plants,” Cell, vol. 156, no. 4. Cell Press, pp. 691–704,
2014.
ista: Gadeyne A, Sánchez Rodríguez C, Vanneste S, Di Rubbo S, Zauber H, Vanneste
K, Van Leene J, De Winne N, Eeckhout D, Persiau G, Van De Slijke E, Cannoot B,
Vercruysse L, Mayers J, Adamowski M, Kania U, Ehrlich M, Schweighofer A, Ketelaar
T, Maere S, Bednarek S, Friml J, Gevaert K, Witters E, Russinova E, Persson S,
De Jaeger G, Van Damme D. 2014. The TPLATE adaptor complex drives clathrin-mediated
endocytosis in plants. Cell. 156(4), 691–704.
mla: Gadeyne, Astrid, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated
Endocytosis in Plants.” Cell, vol. 156, no. 4, Cell Press, 2014, pp. 691–704,
doi:10.1016/j.cell.2014.01.039.
short: A. Gadeyne, C. Sánchez Rodríguez, S. Vanneste, S. Di Rubbo, H. Zauber, K.
Vanneste, J. Van Leene, N. De Winne, D. Eeckhout, G. Persiau, E. Van De Slijke,
B. Cannoot, L. Vercruysse, J. Mayers, M. Adamowski, U. Kania, M. Ehrlich, A. Schweighofer,
T. Ketelaar, S. Maere, S. Bednarek, J. Friml, K. Gevaert, E. Witters, E. Russinova,
S. Persson, G. De Jaeger, D. Van Damme, Cell 156 (2014) 691–704.
date_created: 2018-12-11T11:56:31Z
date_published: 2014-02-13T00:00:00Z
date_updated: 2021-01-12T06:56:13Z
day: '13'
department:
- _id: JiFr
doi: 10.1016/j.cell.2014.01.039
intvolume: ' 156'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 691 - 704
publication: Cell
publication_identifier:
issn:
- '00928674'
publication_status: published
publisher: Cell Press
publist_id: '4721'
quality_controlled: '1'
scopus_import: 1
status: public
title: The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 156
year: '2014'
...