[{"date_updated":"2023-08-02T14:43:50Z","year":"2022","citation":{"ieee":"A. Yanagida <i>et al.</i>, “Cell surface fluctuations regulate early embryonic lineage sorting,” <i>Cell</i>, vol. 185, no. 5. Cell Press, p. 777–793.e20, 2022.","chicago":"Yanagida, Ayaka, Elena Corujo-Simon, Christopher K. Revell, Preeti Sahu, Giuliano G. Stirparo, Irene M. Aspalter, Alex K. Winkel, et al. “Cell Surface Fluctuations Regulate Early Embryonic Lineage Sorting.” <i>Cell</i>. Cell Press, 2022. <a href=\"https://doi.org/10.1016/j.cell.2022.01.022\">https://doi.org/10.1016/j.cell.2022.01.022</a>.","apa":"Yanagida, A., Corujo-Simon, E., Revell, C. K., Sahu, P., Stirparo, G. G., Aspalter, I. M., … Chalut, K. J. (2022). Cell surface fluctuations regulate early embryonic lineage sorting. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2022.01.022\">https://doi.org/10.1016/j.cell.2022.01.022</a>","ama":"Yanagida A, Corujo-Simon E, Revell CK, et al. Cell surface fluctuations regulate early embryonic lineage sorting. <i>Cell</i>. 2022;185(5):777-793.e20. doi:<a href=\"https://doi.org/10.1016/j.cell.2022.01.022\">10.1016/j.cell.2022.01.022</a>","ista":"Yanagida A, Corujo-Simon E, Revell CK, Sahu P, Stirparo GG, Aspalter IM, Winkel AK, Peters R, De Belly H, Cassani DAD, Achouri S, Blumenfeld R, Franze K, Hannezo EB, Paluch EK, Nichols J, Chalut KJ. 2022. Cell surface fluctuations regulate early embryonic lineage sorting. Cell. 185(5), 777–793.e20.","mla":"Yanagida, Ayaka, et al. “Cell Surface Fluctuations Regulate Early Embryonic Lineage Sorting.” <i>Cell</i>, vol. 185, no. 5, Cell Press, 2022, p. 777–793.e20, doi:<a href=\"https://doi.org/10.1016/j.cell.2022.01.022\">10.1016/j.cell.2022.01.022</a>.","short":"A. Yanagida, E. Corujo-Simon, C.K. Revell, P. Sahu, G.G. Stirparo, I.M. Aspalter, A.K. Winkel, R. Peters, H. De Belly, D.A.D. Cassani, S. Achouri, R. Blumenfeld, K. Franze, E.B. Hannezo, E.K. Paluch, J. Nichols, K.J. Chalut, Cell 185 (2022) 777–793.e20."},"isi":1,"external_id":{"isi":["000796293700007"],"pmid":["35196500"]},"doi":"10.1016/j.cell.2022.01.022","day":"22","abstract":[{"text":"In development, lineage segregation is coordinated in time and space. An important example is the mammalian inner cell mass, in which the primitive endoderm (PrE, founder of the yolk sac) physically segregates from the epiblast (EPI, founder of the fetus). While the molecular requirements have been well studied, the physical mechanisms determining spatial segregation between EPI and PrE remain elusive. Here, we investigate the mechanical basis of EPI and PrE sorting. We find that rather than the differences in static cell surface mechanical parameters as in classical sorting models, it is the differences in surface fluctuations that robustly ensure physical lineage sorting. These differential surface fluctuations systematically correlate with differential cellular fluidity, which we propose together constitute a non-equilibrium sorting mechanism for EPI and PrE lineages. By combining experiments and modeling, we identify cell surface dynamics as a key factor orchestrating the correct spatial segregation of the founder embryonic lineages.","lang":"eng"}],"volume":185,"acknowledgement":"We are grateful to H. Niwa for Dox regulatable PB vector; G. Charras for EzrinT567D cDNA; K. Jones for tdTomato ESCs, R26-Confetti ESCs, and laboratory assistance; M. Kinoshita for pPB-CAG-H2B-BFP plasmid; P. Humphreys and D. Clements for imaging support; G. Chu, P. Attlesey, and staff for animal husbandry; S. Pallett for laboratory assistance; C. Mulas for critical feedback on the project; T. Boroviak for single-cell RNA-seq; the EMBL Genomics Core Facility for sequencing; and M. Merkel for developing and sharing the original version of the 3D Voronoi code. This work was financially supported by BBSRC ( BB/Moo4023/1 and BB/T007044/1 to K.J.C. and J.N., Alert16 grant BB/R000042 to E.K.P.), Leverhulme Trust ( RPG-2014-080 to K.J.C. and J.N.), European Research Council ( 772798 -CellFateTech to K.J.C., 311637 -MorphoCorDiv and 820188 -NanoMechShape to E.K.P., Starting Grant 851288 to E.H., and 772426 -MeChemGui to K.F.), the Isaac Newton Trust (to E.K.P.), Medical Research Council UK (MRC program award MC_UU_00012/5 to E.K.P.), the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 641639 ( ITN Biopol , H.D.B. and E.K.P.), the Alexander von Humboldt Foundation (Alexander von Humboldt Professorship to K.F.), EMBO ALTF 522-2021 (to P.S.), Centre for Trophoblast Research (Next Generation fellowship to S.A.), and JSPS Overseas Research Fellowships (to A.Y.). The Wellcome-MRC Cambridge Stem Cell Institute receives core funding from Wellcome Trust ( 203151/Z/16/Z ) and MRC ( MC_PC_17230 ). For the purpose of open access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.","ddc":["570"],"pmid":1,"_id":"10825","scopus_import":"1","author":[{"first_name":"Ayaka","last_name":"Yanagida","full_name":"Yanagida, Ayaka"},{"first_name":"Elena","last_name":"Corujo-Simon","full_name":"Corujo-Simon, Elena"},{"last_name":"Revell","first_name":"Christopher K.","full_name":"Revell, Christopher K."},{"id":"55BA52EE-A185-11EA-88FD-18AD3DDC885E","last_name":"Sahu","first_name":"Preeti","full_name":"Sahu, Preeti"},{"first_name":"Giuliano G.","last_name":"Stirparo","full_name":"Stirparo, Giuliano G."},{"first_name":"Irene M.","last_name":"Aspalter","full_name":"Aspalter, Irene M."},{"full_name":"Winkel, Alex K.","last_name":"Winkel","first_name":"Alex K."},{"first_name":"Ruby","last_name":"Peters","full_name":"Peters, Ruby"},{"full_name":"De Belly, Henry","last_name":"De Belly","first_name":"Henry"},{"first_name":"Davide A.D.","last_name":"Cassani","full_name":"Cassani, Davide A.D."},{"full_name":"Achouri, Sarra","last_name":"Achouri","first_name":"Sarra"},{"full_name":"Blumenfeld, Raphael","last_name":"Blumenfeld","first_name":"Raphael"},{"full_name":"Franze, Kristian","first_name":"Kristian","last_name":"Franze"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","first_name":"Edouard B","last_name":"Hannezo"},{"last_name":"Paluch","first_name":"Ewa K.","full_name":"Paluch, Ewa K."},{"last_name":"Nichols","first_name":"Jennifer","full_name":"Nichols, Jennifer"},{"first_name":"Kevin J.","last_name":"Chalut","full_name":"Chalut, Kevin J."}],"issue":"5","publication_status":"published","department":[{"_id":"EdHa"}],"date_created":"2022-03-06T23:01:52Z","article_processing_charge":"No","title":"Cell surface fluctuations regulate early embryonic lineage sorting","intvolume":"       185","page":"777-793.e20","ec_funded":1,"quality_controlled":"1","file_date_updated":"2022-03-07T07:55:23Z","publisher":"Cell Press","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_published":"2022-02-22T00:00:00Z","type":"journal_article","publication_identifier":{"issn":["00928674"],"eissn":["10974172"]},"oa":1,"file":[{"date_created":"2022-03-07T07:55:23Z","checksum":"ae305060e8031297771b89dae9e36a29","file_size":8478995,"date_updated":"2022-03-07T07:55:23Z","content_type":"application/pdf","file_name":"2022_Cell_Yanagida.pdf","access_level":"open_access","success":1,"relation":"main_file","file_id":"10831","creator":"dernst"}],"status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication":"Cell","has_accepted_license":"1","oa_version":"Published Version","project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"month":"02","language":[{"iso":"eng"}]},{"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"oa_version":"Published Version","project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"},{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020"},{"call_identifier":"FWF","_id":"2693FD8C-B435-11E9-9278-68D0E5697425","name":"Tissue material properties in embryonic development","grant_number":"V00736"}],"month":"04","publication":"Cell","has_accepted_license":"1","file":[{"creator":"cziletti","file_id":"9534","success":1,"relation":"main_file","access_level":"open_access","file_name":"2021_Cell_Petridou.pdf","content_type":"application/pdf","date_updated":"2021-06-08T10:04:10Z","file_size":11405875,"checksum":"1e5295fbd9c2a459173ec45a0e8a7c2e","date_created":"2021-06-08T10:04:10Z"}],"status":"public","related_material":{"link":[{"url":"https://ist.ac.at/en/news/embryonic-tissue-undergoes-phase-transition/","relation":"press_release","description":"News on IST Homepage"}]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_identifier":{"eissn":["10974172"],"issn":["00928674"]},"oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_published":"2021-04-01T00:00:00Z","type":"journal_article","publisher":"Elsevier","article_type":"original","page":"1914-1928.e19","ec_funded":1,"quality_controlled":"1","file_date_updated":"2021-06-08T10:04:10Z","publication_status":"published","date_created":"2021-04-11T22:01:14Z","article_processing_charge":"No","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"title":"Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions","intvolume":"       184","_id":"9316","pmid":1,"scopus_import":"1","author":[{"last_name":"Petridou","first_name":"Nicoletta","full_name":"Petridou, Nicoletta","orcid":"0000-0002-8451-1195","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Corominas-Murtra, Bernat","orcid":"0000-0001-9806-5643","last_name":"Corominas-Murtra","first_name":"Bernat","id":"43BE2298-F248-11E8-B48F-1D18A9856A87"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"}],"issue":"7","acknowledgement":"We thank Carl Goodrich and the members of the Heisenberg and Hannezo groups, in particular Reka Korei, for help, technical advice, and discussions; and the Bioimaging and zebrafish facilities of the IST Austria for continuous support. This work was supported by the Elise Richter Program of Austrian Science Fund (FWF) to N.I.P. ( V 736-B26 ) and the European Union (European Research Council Advanced Grant 742573 to C.-P.H. and European Research Council Starting Grant 851288 to E.H.).","volume":184,"ddc":["570"],"doi":"10.1016/j.cell.2021.02.017","day":"01","abstract":[{"lang":"eng","text":"Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural PTs of embryonic tissues from local cell connectivity. By using percolation theory, combined with directly monitoring dynamic changes in tissue rheology and cell contact mechanics, we demonstrate that the zebrafish blastoderm undergoes a genuine rigidity PT, brought about by a small reduction in adhesion-dependent cell connectivity below a critical value. We quantitatively predict and experimentally verify hallmarks of PTs, including power-law exponents and associated discontinuities of macroscopic observables. Finally, we show that this uniform PT depends on blastoderm cells undergoing meta-synchronous divisions causing random and, consequently, uniform changes in cell connectivity. Collectively, our theoretical and experimental findings reveal the structural basis of material PTs in an organismal context."}],"date_updated":"2023-08-07T14:33:59Z","year":"2021","citation":{"chicago":"Petridou, Nicoletta, Bernat Corominas-Murtra, Carl-Philipp J Heisenberg, and Edouard B Hannezo. “Rigidity Percolation Uncovers a Structural Basis for Embryonic Tissue Phase Transitions.” <i>Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.cell.2021.02.017\">https://doi.org/10.1016/j.cell.2021.02.017</a>.","ieee":"N. Petridou, B. Corominas-Murtra, C.-P. J. Heisenberg, and E. B. Hannezo, “Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions,” <i>Cell</i>, vol. 184, no. 7. Elsevier, p. 1914–1928.e19, 2021.","apa":"Petridou, N., Corominas-Murtra, B., Heisenberg, C.-P. J., &#38; Hannezo, E. B. (2021). Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2021.02.017\">https://doi.org/10.1016/j.cell.2021.02.017</a>","ama":"Petridou N, Corominas-Murtra B, Heisenberg C-PJ, Hannezo EB. Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. <i>Cell</i>. 2021;184(7):1914-1928.e19. doi:<a href=\"https://doi.org/10.1016/j.cell.2021.02.017\">10.1016/j.cell.2021.02.017</a>","ista":"Petridou N, Corominas-Murtra B, Heisenberg C-PJ, Hannezo EB. 2021. Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. Cell. 184(7), 1914–1928.e19.","short":"N. Petridou, B. Corominas-Murtra, C.-P.J. Heisenberg, E.B. Hannezo, Cell 184 (2021) 1914–1928.e19.","mla":"Petridou, Nicoletta, et al. “Rigidity Percolation Uncovers a Structural Basis for Embryonic Tissue Phase Transitions.” <i>Cell</i>, vol. 184, no. 7, Elsevier, 2021, p. 1914–1928.e19, doi:<a href=\"https://doi.org/10.1016/j.cell.2021.02.017\">10.1016/j.cell.2021.02.017</a>."},"isi":1,"external_id":{"isi":["000636734000022"],"pmid":["33730596"]}},{"status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file":[{"access_level":"open_access","relation":"main_file","file_id":"7795","creator":"dernst","date_created":"2020-05-04T10:20:55Z","file_size":17992888,"checksum":"e2114902f4e9d75a752e9efb5ae06011","date_updated":"2020-07-14T12:48:03Z","content_type":"application/pdf","file_name":"2020_Cell_Dekoninck.pdf"}],"type":"journal_article","date_published":"2020-04-30T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"publication_identifier":{"eissn":["10974172"],"issn":["00928674"]},"language":[{"iso":"eng"}],"has_accepted_license":"1","publication":"Cell","month":"04","oa_version":"Published Version","ddc":["570"],"volume":181,"external_id":{"isi":["000530708400016"],"pmid":["32259486"]},"isi":1,"year":"2020","citation":{"ista":"Dekoninck S, Hannezo EB, Sifrim A, Miroshnikova YA, Aragona M, Malfait M, Gargouri S, De Neunheuser C, Dubois C, Voet T, Wickström SA, Simons BD, Blanpain C. 2020. Defining the design principles of skin epidermis postnatal growth. Cell. 181(3), 604–620.e22.","short":"S. Dekoninck, E.B. Hannezo, A. Sifrim, Y.A. Miroshnikova, M. Aragona, M. Malfait, S. Gargouri, C. De Neunheuser, C. Dubois, T. Voet, S.A. Wickström, B.D. Simons, C. Blanpain, Cell 181 (2020) 604–620.e22.","mla":"Dekoninck, Sophie, et al. “Defining the Design Principles of Skin Epidermis Postnatal Growth.” <i>Cell</i>, vol. 181, no. 3, Elsevier, 2020, p. 604–620.e22, doi:<a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">10.1016/j.cell.2020.03.015</a>.","ieee":"S. Dekoninck <i>et al.</i>, “Defining the design principles of skin epidermis postnatal growth,” <i>Cell</i>, vol. 181, no. 3. Elsevier, p. 604–620.e22, 2020.","chicago":"Dekoninck, Sophie, Edouard B Hannezo, Alejandro Sifrim, Yekaterina A. Miroshnikova, Mariaceleste Aragona, Milan Malfait, Souhir Gargouri, et al. “Defining the Design Principles of Skin Epidermis Postnatal Growth.” <i>Cell</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">https://doi.org/10.1016/j.cell.2020.03.015</a>.","apa":"Dekoninck, S., Hannezo, E. B., Sifrim, A., Miroshnikova, Y. A., Aragona, M., Malfait, M., … Blanpain, C. (2020). Defining the design principles of skin epidermis postnatal growth. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">https://doi.org/10.1016/j.cell.2020.03.015</a>","ama":"Dekoninck S, Hannezo EB, Sifrim A, et al. Defining the design principles of skin epidermis postnatal growth. <i>Cell</i>. 2020;181(3):604-620.e22. doi:<a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">10.1016/j.cell.2020.03.015</a>"},"date_updated":"2023-08-21T06:17:43Z","abstract":[{"lang":"eng","text":"During embryonic and postnatal development, organs and tissues grow steadily to achieve their final size at the end of puberty. However, little is known about the cellular dynamics that mediate postnatal growth. By combining in vivo clonal lineage tracing, proliferation kinetics, single-cell transcriptomics, andin vitro micro-pattern experiments, we resolved the cellular dynamics taking place during postnatal skin epidermis expansion. Our data revealed that harmonious growth is engineered by a single population of developmental progenitors presenting a fixed fate imbalance of self-renewing divisions with an ever-decreasing proliferation rate. Single-cell RNA sequencing revealed that epidermal developmental progenitors form a more uniform population compared with adult stem and progenitor cells. Finally, we found that the spatial pattern of cell division orientation is dictated locally by the underlying collagen fiber orientation. Our results uncover a simple design principle of organ growth where progenitors and differentiated cells expand in harmony with their surrounding tissues."}],"day":"30","doi":"10.1016/j.cell.2020.03.015","file_date_updated":"2020-07-14T12:48:03Z","quality_controlled":"1","page":"604-620.e22","article_type":"original","publisher":"Elsevier","issue":"3","author":[{"full_name":"Dekoninck, Sophie","last_name":"Dekoninck","first_name":"Sophie"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"last_name":"Sifrim","first_name":"Alejandro","full_name":"Sifrim, Alejandro"},{"full_name":"Miroshnikova, Yekaterina A.","first_name":"Yekaterina A.","last_name":"Miroshnikova"},{"first_name":"Mariaceleste","last_name":"Aragona","full_name":"Aragona, Mariaceleste"},{"full_name":"Malfait, Milan","last_name":"Malfait","first_name":"Milan"},{"full_name":"Gargouri, Souhir","last_name":"Gargouri","first_name":"Souhir"},{"last_name":"De Neunheuser","first_name":"Charlotte","full_name":"De Neunheuser, Charlotte"},{"first_name":"Christine","last_name":"Dubois","full_name":"Dubois, Christine"},{"last_name":"Voet","first_name":"Thierry","full_name":"Voet, Thierry"},{"full_name":"Wickström, Sara A.","last_name":"Wickström","first_name":"Sara A."},{"full_name":"Simons, Benjamin D.","first_name":"Benjamin D.","last_name":"Simons"},{"first_name":"Cédric","last_name":"Blanpain","full_name":"Blanpain, Cédric"}],"scopus_import":"1","_id":"7789","pmid":1,"intvolume":"       181","title":"Defining the design principles of skin epidermis postnatal growth","date_created":"2020-05-03T22:00:48Z","department":[{"_id":"EdHa"}],"article_processing_charge":"No","publication_status":"published"},{"abstract":[{"lang":"eng","text":"A process of restorative patterning in plant roots correctly replaces eliminated cells to heal local injuries despite the absence of cell migration, which underpins wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell divisions and acquisition of specific cell identities. Plants regularly endure wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary abilities to restore their tissues after injuries. Here, we provide insight into a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted elimination of different cells in Arabidopsis root combined with live-imaging tracking during vertical growth allowed analysis of the regeneration processes in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated their stem cell transcriptional programs. They accelerated their progression through cell cycle, coordinately changed the cell division orientation, and ultimately acquired de novo the correct cell fates to replace missing cells. These observations highlight existence of unknown intercellular positional signaling and demonstrate the capability of specified cells to re-acquire stem cell programs as a crucial part of the plant-specific mechanism of wound healing."}],"day":"02","doi":"10.1016/j.cell.2019.04.015","external_id":{"pmid":["31051107"],"isi":["000466843000015"]},"isi":1,"year":"2019","citation":{"ista":"Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4), 957–969.e13.","mla":"Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing.” <i>Cell</i>, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13, doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">10.1016/j.cell.2019.04.015</a>.","short":"P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell 177 (2019) 957–969.e13.","ieee":"P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml, “Re-activation of stem cell pathways for pattern restoration in plant wound healing,” <i>Cell</i>, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019.","chicago":"Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková, and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing.” <i>Cell</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">https://doi.org/10.1016/j.cell.2019.04.015</a>.","ama":"Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation of stem cell pathways for pattern restoration in plant wound healing. <i>Cell</i>. 2019;177(4):957-969.e13. doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">10.1016/j.cell.2019.04.015</a>","apa":"Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., &#38; Friml, J. (2019). Re-activation of stem cell pathways for pattern restoration in plant wound healing. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">https://doi.org/10.1016/j.cell.2019.04.015</a>"},"date_updated":"2024-03-25T23:30:06Z","ddc":["570"],"volume":177,"intvolume":"       177","title":"Re-activation of stem cell pathways for pattern restoration in plant wound healing","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"date_created":"2019-04-28T21:59:14Z","article_processing_charge":"No","publication_status":"published","issue":"4","author":[{"full_name":"Marhavá, Petra","first_name":"Petra","last_name":"Marhavá","id":"44E59624-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Hörmayer, Lukas","orcid":"0000-0001-8295-2926","last_name":"Hörmayer","first_name":"Lukas","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87"},{"id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko","last_name":"Yoshida","full_name":"Yoshida, Saiko"},{"full_name":"Marhavy, Peter","orcid":"0000-0001-5227-5741","last_name":"Marhavy","first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"scopus_import":"1","pmid":1,"_id":"6351","publisher":"Elsevier","file_date_updated":"2020-07-14T12:47:28Z","ec_funded":1,"quality_controlled":"1","page":"957-969.e13","oa":1,"publication_identifier":{"eissn":["10974172"],"issn":["00928674"]},"type":"journal_article","date_published":"2019-05-02T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","related_material":{"link":[{"url":"https://ist.ac.at/en/news/specialized-plant-cells-regain-stem-cell-features-to-heal-wounds/","relation":"press_release","description":"News on IST Homepage"}],"record":[{"relation":"dissertation_contains","id":"9992","status":"public"}]},"file":[{"creator":"dernst","file_id":"6411","relation":"main_file","access_level":"open_access","file_name":"2019_Cell_Marhava.pdf","content_type":"application/pdf","date_updated":"2020-07-14T12:47:28Z","file_size":10272032,"checksum":"4ceba04a96a74f5092ec3ce2c579a0c7","date_created":"2019-05-13T06:12:45Z"}],"month":"05","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"acknowledged_ssus":[{"_id":"Bio"}],"oa_version":"Published Version","has_accepted_license":"1","publication":"Cell","language":[{"iso":"eng"}]},{"language":[{"iso":"eng"}],"month":"05","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"grant_number":"P31639","name":"Active mechano-chemical description of the cell cytoskeleton","_id":"268294B6-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"oa_version":"Published Version","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"has_accepted_license":"1","publication":"Cell","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","status":"public","related_material":{"link":[{"url":"https://ist.ac.at/en/news/how-the-cytoplasm-separates-from-the-yolk/","description":"News on IST Homepage","relation":"press_release"}],"record":[{"relation":"dissertation_contains","id":"8350","status":"public"}]},"file":[{"success":1,"access_level":"open_access","relation":"main_file","file_id":"8686","creator":"dernst","date_created":"2020-10-21T07:22:34Z","checksum":"aea43726d80e35ce3885073a5f05c3e3","file_size":3356292,"date_updated":"2020-10-21T07:22:34Z","content_type":"application/pdf","file_name":"2019_Cell_Shamipour_accepted.pdf"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.cell.2019.04.030"}],"oa":1,"publication_identifier":{"eissn":["10974172"],"issn":["00928674"]},"type":"journal_article","date_published":"2019-05-30T00:00:00Z","article_type":"original","publisher":"Elsevier","file_date_updated":"2020-10-21T07:22:34Z","ec_funded":1,"quality_controlled":"1","page":"1463-1479.e18","intvolume":"       177","title":"Bulk actin dynamics drive phase segregation in zebrafish oocytes","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"BjHo"}],"date_created":"2019-06-02T21:59:12Z","article_processing_charge":"No","publication_status":"published","issue":"6","author":[{"full_name":"Shamipour, Shayan","last_name":"Shamipour","first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87"},{"id":"4039350E-F248-11E8-B48F-1D18A9856A87","full_name":"Kardos, Roland","first_name":"Roland","last_name":"Kardos"},{"full_name":"Xue, Shi-lei","last_name":"Xue","first_name":"Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87"},{"id":"3A374330-F248-11E8-B48F-1D18A9856A87","last_name":"Hof","first_name":"Björn","full_name":"Hof, Björn","orcid":"0000-0003-2057-2754"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","first_name":"Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"scopus_import":"1","pmid":1,"_id":"6508","ddc":["570"],"acknowledgement":"We would like to thank Pierre Recho, Guillaume Salbreux, and Silvia Grigolon for advice on the theory, Lila Solnica-Krezel for kindly providing us with zebrafish dachsous mutants, members of the Heisenberg and Hannezo groups for fruitful discussions, and the Bioimaging and zebrafish facilities at IST Austria for their continuous support. This project has received funding from the European Union (European Research Council Advanced Grant 742573 to C.P.H.) and from the Austrian Science Fund (FWF) (P 31639 to E.H.).","volume":177,"abstract":[{"lang":"eng","text":"Segregation of maternal determinants within the oocyte constitutes the first step in embryo patterning. In zebrafish oocytes, extensive ooplasmic streaming leads to the segregation of ooplasm from yolk granules along the animal-vegetal axis of the oocyte. Here, we show that this process does not rely on cortical actin reorganization, as previously thought, but instead on a cell-cycle-dependent bulk actin polymerization wave traveling from the animal to the vegetal pole of the oocyte. This wave functions in segregation by both pulling ooplasm animally and pushing yolk granules vegetally. Using biophysical experimentation and theory, we show that ooplasm pulling is mediated by bulk actin network flows exerting friction forces on the ooplasm, while yolk granule pushing is achieved by a mechanism closely resembling actin comet formation on yolk granules. Our study defines a novel role of cell-cycle-controlled bulk actin polymerization waves in oocyte polarization via ooplasmic segregation."}],"day":"30","doi":"10.1016/j.cell.2019.04.030","external_id":{"isi":["000469415100013"],"pmid":["31080065"]},"isi":1,"year":"2019","citation":{"ista":"Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. 2019. Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. 177(6), 1463–1479.e18.","mla":"Shamipour, Shayan, et al. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes.” <i>Cell</i>, vol. 177, no. 6, Elsevier, 2019, p. 1463–1479.e18, doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.030\">10.1016/j.cell.2019.04.030</a>.","short":"S. Shamipour, R. Kardos, S. Xue, B. Hof, E.B. Hannezo, C.-P.J. Heisenberg, Cell 177 (2019) 1463–1479.e18.","ieee":"S. Shamipour, R. Kardos, S. Xue, B. Hof, E. B. Hannezo, and C.-P. J. Heisenberg, “Bulk actin dynamics drive phase segregation in zebrafish oocytes,” <i>Cell</i>, vol. 177, no. 6. Elsevier, p. 1463–1479.e18, 2019.","chicago":"Shamipour, Shayan, Roland Kardos, Shi-lei Xue, Björn Hof, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes.” <i>Cell</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.cell.2019.04.030\">https://doi.org/10.1016/j.cell.2019.04.030</a>.","ama":"Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. Bulk actin dynamics drive phase segregation in zebrafish oocytes. <i>Cell</i>. 2019;177(6):1463-1479.e18. doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.030\">10.1016/j.cell.2019.04.030</a>","apa":"Shamipour, S., Kardos, R., Xue, S., Hof, B., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2019). Bulk actin dynamics drive phase segregation in zebrafish oocytes. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2019.04.030\">https://doi.org/10.1016/j.cell.2019.04.030</a>"},"date_updated":"2024-03-25T23:30:21Z"},{"language":[{"iso":"eng"}],"publication":"Cell","month":"07","oa_version":"Published Version","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639","_id":"268294B6-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","status":"public","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.cell.2019.05.052"}],"date_published":"2019-07-27T00:00:00Z","type":"journal_article","oa":1,"publication_identifier":{"issn":["00928674"]},"page":"12-25","quality_controlled":"1","ec_funded":1,"article_type":"review","publisher":"Elsevier","author":[{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"issue":"1","pmid":1,"_id":"6601","scopus_import":"1","title":"Mechanochemical feedback loops in development and disease","intvolume":"       178","publication_status":"published","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"article_processing_charge":"No","date_created":"2019-06-30T21:59:11Z","volume":178,"isi":1,"external_id":{"pmid":["31251912"],"isi":["000473002700005"]},"date_updated":"2023-08-28T12:25:21Z","year":"2019","citation":{"apa":"Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2019). Mechanochemical feedback loops in development and disease. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2019.05.052\">https://doi.org/10.1016/j.cell.2019.05.052</a>","ama":"Hannezo EB, Heisenberg C-PJ. Mechanochemical feedback loops in development and disease. <i>Cell</i>. 2019;178(1):12-25. doi:<a href=\"https://doi.org/10.1016/j.cell.2019.05.052\">10.1016/j.cell.2019.05.052</a>","ieee":"E. B. Hannezo and C.-P. J. Heisenberg, “Mechanochemical feedback loops in development and disease,” <i>Cell</i>, vol. 178, no. 1. Elsevier, pp. 12–25, 2019.","chicago":"Hannezo, Edouard B, and Carl-Philipp J Heisenberg. “Mechanochemical Feedback Loops in Development and Disease.” <i>Cell</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.cell.2019.05.052\">https://doi.org/10.1016/j.cell.2019.05.052</a>.","short":"E.B. Hannezo, C.-P.J. Heisenberg, Cell 178 (2019) 12–25.","mla":"Hannezo, Edouard B., and Carl-Philipp J. Heisenberg. “Mechanochemical Feedback Loops in Development and Disease.” <i>Cell</i>, vol. 178, no. 1, Elsevier, 2019, pp. 12–25, doi:<a href=\"https://doi.org/10.1016/j.cell.2019.05.052\">10.1016/j.cell.2019.05.052</a>.","ista":"Hannezo EB, Heisenberg C-PJ. 2019. Mechanochemical feedback loops in development and disease. Cell. 178(1), 12–25."},"abstract":[{"text":"There is increasing evidence that both mechanical and biochemical signals play important roles in development and disease. The development of complex organisms, in particular, has been proposed to rely on the feedback between mechanical and biochemical patterning events. This feedback occurs at the molecular level via mechanosensation but can also arise as an emergent property of the system at the cellular and tissue level. In recent years, dynamic changes in tissue geometry, flow, rheology, and cell fate specification have emerged as key platforms of mechanochemical feedback loops in multiple processes. Here, we review recent experimental and theoretical advances in understanding how these feedbacks function in development and disease.","lang":"eng"}],"doi":"10.1016/j.cell.2019.05.052","day":"27"},{"_id":"803","scopus_import":"1","author":[{"full_name":"Samwer, Matthias","last_name":"Samwer","first_name":"Matthias"},{"full_name":"Schneider, Maximilian","last_name":"Schneider","first_name":"Maximilian"},{"full_name":"Hoefler, Rudolf","first_name":"Rudolf","last_name":"Hoefler"},{"id":"309D50DA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5795-0133","full_name":"Schmalhorst, Philipp S","first_name":"Philipp S","last_name":"Schmalhorst"},{"first_name":"Julian","last_name":"Jude","full_name":"Jude, Julian"},{"full_name":"Zuber, Johannes","first_name":"Johannes","last_name":"Zuber"},{"full_name":"Gerlic, Daniel","last_name":"Gerlic","first_name":"Daniel"}],"issue":"5","publication_status":"published","date_created":"2018-12-11T11:48:35Z","department":[{"_id":"CaHe"}],"article_processing_charge":"No","title":"DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes","intvolume":"       170","page":"956 - 972","quality_controlled":"1","file_date_updated":"2020-07-14T12:48:08Z","publisher":"Cell Press","date_updated":"2023-09-27T10:59:14Z","year":"2017","citation":{"short":"M. Samwer, M. Schneider, R. Hoefler, P.S. Schmalhorst, J. Jude, J. Zuber, D. Gerlic, Cell 170 (2017) 956–972.","mla":"Samwer, Matthias, et al. “DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.” <i>Cell</i>, vol. 170, no. 5, Cell Press, 2017, pp. 956–72, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.07.038\">10.1016/j.cell.2017.07.038</a>.","ista":"Samwer M, Schneider M, Hoefler R, Schmalhorst PS, Jude J, Zuber J, Gerlic D. 2017. DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes. Cell. 170(5), 956–972.","ama":"Samwer M, Schneider M, Hoefler R, et al. DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes. <i>Cell</i>. 2017;170(5):956-972. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.07.038\">10.1016/j.cell.2017.07.038</a>","apa":"Samwer, M., Schneider, M., Hoefler, R., Schmalhorst, P. S., Jude, J., Zuber, J., &#38; Gerlic, D. (2017). DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.07.038\">https://doi.org/10.1016/j.cell.2017.07.038</a>","ieee":"M. Samwer <i>et al.</i>, “DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes,” <i>Cell</i>, vol. 170, no. 5. Cell Press, pp. 956–972, 2017.","chicago":"Samwer, Matthias, Maximilian Schneider, Rudolf Hoefler, Philipp S Schmalhorst, Julian Jude, Johannes Zuber, and Daniel Gerlic. “DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.” <i>Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.07.038\">https://doi.org/10.1016/j.cell.2017.07.038</a>."},"isi":1,"external_id":{"isi":["000408372400014"]},"doi":"10.1016/j.cell.2017.07.038","day":"24","abstract":[{"lang":"eng","text":"Eukaryotic cells store their chromosomes in a single nucleus. This is important to maintain genomic integrity, as chromosomes packaged into separate nuclei (micronuclei) are prone to massive DNA damage. During mitosis, higher eukaryotes disassemble their nucleus and release individualized chromosomes for segregation. How numerous chromosomes subsequently reform a single nucleus has remained unclear. Using image-based screening of human cells, we identified barrier-to-autointegration factor (BAF) as a key factor guiding membranes to form a single nucleus. Unexpectedly, nuclear assembly does not require BAF?s association with inner nuclear membrane proteins but instead relies on BAF?s ability to bridge distant DNA sites. Live-cell imaging and in vitro reconstitution showed that BAF enriches around the mitotic chromosome ensemble to induce a densely cross-bridged chromatin layer that is mechanically stiff and limits membranes to the surface. Our study reveals that BAF-mediated changes in chromosome mechanics underlie nuclear assembly with broad implications for proper genome function."}],"volume":170,"ddc":["570"],"publication":"Cell","has_accepted_license":"1","acknowledged_ssus":[{"_id":"Bio"}],"oa_version":"Published Version","month":"08","language":[{"iso":"eng"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png"},"date_published":"2017-08-24T00:00:00Z","type":"journal_article","publication_identifier":{"issn":["00928674"]},"oa":1,"publist_id":"6848","file":[{"file_id":"5852","creator":"dernst","relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:48:08Z","content_type":"application/pdf","file_name":"2017_Cell_Samwer.pdf","date_created":"2019-01-18T13:45:40Z","file_size":17666637,"checksum":"64897b0c5373f22273f598e4672c60ff"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public"},{"isi":1,"external_id":{"isi":["000411331800024"]},"date_updated":"2023-09-28T11:34:17Z","citation":{"ieee":"E. B. Hannezo <i>et al.</i>, “A unifying theory of branching morphogenesis,” <i>Cell</i>, vol. 171, no. 1. Cell Press, pp. 242–255, 2017.","chicago":"Hannezo, Edouard B, Colinda Scheele, Mohammad Moad, Nicholas Drogo, Rakesh Heer, Rosemary Sampogna, Jacco Van Rheenen, and Benjamin Simons. “A Unifying Theory of Branching Morphogenesis.” <i>Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.08.026\">https://doi.org/10.1016/j.cell.2017.08.026</a>.","ama":"Hannezo EB, Scheele C, Moad M, et al. A unifying theory of branching morphogenesis. <i>Cell</i>. 2017;171(1):242-255. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.08.026\">10.1016/j.cell.2017.08.026</a>","apa":"Hannezo, E. B., Scheele, C., Moad, M., Drogo, N., Heer, R., Sampogna, R., … Simons, B. (2017). A unifying theory of branching morphogenesis. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.08.026\">https://doi.org/10.1016/j.cell.2017.08.026</a>","ista":"Hannezo EB, Scheele C, Moad M, Drogo N, Heer R, Sampogna R, Van Rheenen J, Simons B. 2017. A unifying theory of branching morphogenesis. Cell. 171(1), 242–255.","mla":"Hannezo, Edouard B., et al. “A Unifying Theory of Branching Morphogenesis.” <i>Cell</i>, vol. 171, no. 1, Cell Press, 2017, pp. 242–55, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.08.026\">10.1016/j.cell.2017.08.026</a>.","short":"E.B. Hannezo, C. Scheele, M. Moad, N. Drogo, R. Heer, R. Sampogna, J. Van Rheenen, B. Simons, Cell 171 (2017) 242–255."},"year":"2017","abstract":[{"text":"The morphogenesis of branched organs remains a subject of abiding interest. Although much is known about the underlying signaling pathways, it remains unclear how macroscopic features of branched organs, including their size, network topology, and spatial patterning, are encoded. Here, we show that, in mouse mammary gland, kidney, and human prostate, these features can be explained quantitatively within a single unifying framework of branching and annihilating random walks. Based on quantitative analyses of large-scale organ reconstructions and proliferation kinetics measurements, we propose that morphogenesis follows from the proliferative activity of equipotent tips that stochastically branch and randomly explore their environment but compete neutrally for space, becoming proliferatively inactive when in proximity with neighboring ducts. These results show that complex branched epithelial structures develop as a self-organized process, reliant upon a strikingly simple but generic rule, without recourse to a rigid and deterministic sequence of genetically programmed events.","lang":"eng"}],"doi":"10.1016/j.cell.2017.08.026","day":"21","ddc":["539"],"volume":171,"author":[{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"full_name":"Scheele, Colinda","first_name":"Colinda","last_name":"Scheele"},{"first_name":"Mohammad","last_name":"Moad","full_name":"Moad, Mohammad"},{"full_name":"Drogo, Nicholas","first_name":"Nicholas","last_name":"Drogo"},{"last_name":"Heer","first_name":"Rakesh","full_name":"Heer, Rakesh"},{"full_name":"Sampogna, Rosemary","last_name":"Sampogna","first_name":"Rosemary"},{"full_name":"Van Rheenen, Jacco","last_name":"Van Rheenen","first_name":"Jacco"},{"last_name":"Simons","first_name":"Benjamin","full_name":"Simons, Benjamin"}],"issue":"1","_id":"726","scopus_import":"1","pubrep_id":"883","title":"A unifying theory of branching morphogenesis","intvolume":"       171","publication_status":"published","department":[{"_id":"EdHa"}],"date_created":"2018-12-11T11:48:10Z","article_processing_charge":"No","file_date_updated":"2020-07-14T12:47:55Z","page":"242 - 255","quality_controlled":"1","publisher":"Cell Press","date_published":"2017-09-21T00:00:00Z","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publist_id":"6952","oa":1,"publication_identifier":{"issn":["00928674"]},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","file":[{"date_updated":"2020-07-14T12:47:55Z","file_name":"IST-2017-883-v1+1_PIIS0092867417309510.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:11:17Z","file_size":12670204,"checksum":"7a036d93a9e2e597af9bb504d6133aca","file_id":"4870","creator":"system","relation":"main_file","access_level":"open_access"}],"publication":"Cell","has_accepted_license":"1","month":"09","oa_version":"Published Version","language":[{"iso":"eng"}]},{"publication_identifier":{"issn":["00928674"]},"publist_id":"6951","date_published":"2017-09-21T00:00:00Z","type":"journal_article","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","oa_version":"None","acknowledged_ssus":[{"_id":"ScienComp"}],"project":[{"_id":"25AD6156-B435-11E9-9278-68D0E5697425","grant_number":"LS13-029","name":"Modeling of Polarization and Motility of Leukocytes in Three-Dimensional Environments"},{"grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"month":"09","publication":"Cell","language":[{"iso":"eng"}],"doi":"10.1016/j.cell.2017.07.051","day":"21","abstract":[{"lang":"eng","text":"Actin filaments polymerizing against membranes power endocytosis, vesicular traffic, and cell motility. In vitro reconstitution studies suggest that the structure and the dynamics of actin networks respond to mechanical forces. We demonstrate that lamellipodial actin of migrating cells responds to mechanical load when membrane tension is modulated. In a steady state, migrating cell filaments assume the canonical dendritic geometry, defined by Arp2/3-generated 70° branch points. Increased tension triggers a dense network with a broadened range of angles, whereas decreased tension causes a shift to a sparse configuration dominated by filaments growing perpendicularly to the plasma membrane. We show that these responses emerge from the geometry of branched actin: when load per filament decreases, elongation speed increases and perpendicular filaments gradually outcompete others because they polymerize the shortest distance to the membrane, where they are protected from capping. This network-intrinsic geometrical adaptation mechanism tunes protrusive force in response to mechanical load."}],"date_updated":"2023-09-28T11:33:49Z","year":"2017","citation":{"ista":"Mueller J, Szep G, Nemethova M, de Vries I, Lieber A, Winkler C, Kruse K, Small J, Schmeiser C, Keren K, Hauschild R, Sixt MK. 2017. Load adaptation of lamellipodial actin networks. Cell. 171(1), 188–200.","short":"J. Mueller, G. Szep, M. Nemethova, I. de Vries, A. Lieber, C. Winkler, K. Kruse, J. Small, C. Schmeiser, K. Keren, R. Hauschild, M.K. Sixt, Cell 171 (2017) 188–200.","mla":"Mueller, Jan, et al. “Load Adaptation of Lamellipodial Actin Networks.” <i>Cell</i>, vol. 171, no. 1, Cell Press, 2017, pp. 188–200, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.07.051\">10.1016/j.cell.2017.07.051</a>.","ieee":"J. Mueller <i>et al.</i>, “Load adaptation of lamellipodial actin networks,” <i>Cell</i>, vol. 171, no. 1. Cell Press, pp. 188–200, 2017.","chicago":"Mueller, Jan, Gregory Szep, Maria Nemethova, Ingrid de Vries, Arnon Lieber, Christoph Winkler, Karsten Kruse, et al. “Load Adaptation of Lamellipodial Actin Networks.” <i>Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.07.051\">https://doi.org/10.1016/j.cell.2017.07.051</a>.","ama":"Mueller J, Szep G, Nemethova M, et al. Load adaptation of lamellipodial actin networks. <i>Cell</i>. 2017;171(1):188-200. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.07.051\">10.1016/j.cell.2017.07.051</a>","apa":"Mueller, J., Szep, G., Nemethova, M., de Vries, I., Lieber, A., Winkler, C., … Sixt, M. K. (2017). Load adaptation of lamellipodial actin networks. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.07.051\">https://doi.org/10.1016/j.cell.2017.07.051</a>"},"isi":1,"external_id":{"isi":["000411331800020"]},"volume":171,"publication_status":"published","department":[{"_id":"MiSi"},{"_id":"Bio"}],"date_created":"2018-12-11T11:48:10Z","article_processing_charge":"No","title":"Load adaptation of lamellipodial actin networks","intvolume":"       171","_id":"727","scopus_import":"1","author":[{"last_name":"Mueller","first_name":"Jan","full_name":"Mueller, Jan"},{"id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","last_name":"Szep","first_name":"Gregory","full_name":"Szep, Gregory"},{"full_name":"Nemethova, Maria","first_name":"Maria","last_name":"Nemethova","id":"34E27F1C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Ingrid","last_name":"De Vries","full_name":"De Vries, Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Lieber","first_name":"Arnon","full_name":"Lieber, Arnon"},{"first_name":"Christoph","last_name":"Winkler","full_name":"Winkler, Christoph"},{"full_name":"Kruse, Karsten","first_name":"Karsten","last_name":"Kruse"},{"full_name":"Small, John","last_name":"Small","first_name":"John"},{"full_name":"Schmeiser, Christian","last_name":"Schmeiser","first_name":"Christian"},{"full_name":"Keren, Kinneret","last_name":"Keren","first_name":"Kinneret"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","last_name":"Hauschild","first_name":"Robert"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"issue":"1","publisher":"Cell Press","page":"188 - 200","quality_controlled":"1","ec_funded":1},{"page":"1368 - 1382","ec_funded":1,"quality_controlled":"1","publisher":"Cell Press","_id":"571","scopus_import":1,"author":[{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723","last_name":"Gärtner","first_name":"Florian R"},{"full_name":"Ahmad, Zerkah","first_name":"Zerkah","last_name":"Ahmad"},{"last_name":"Rosenberger","first_name":"Gerhild","full_name":"Rosenberger, Gerhild"},{"last_name":"Fan","first_name":"Shuxia","full_name":"Fan, Shuxia"},{"full_name":"Nicolai, Leo","last_name":"Nicolai","first_name":"Leo"},{"first_name":"Benjamin","last_name":"Busch","full_name":"Busch, Benjamin"},{"first_name":"Gökce","last_name":"Yavuz","full_name":"Yavuz, Gökce"},{"first_name":"Manja","last_name":"Luckner","full_name":"Luckner, Manja"},{"full_name":"Ishikawa Ankerhold, Hellen","last_name":"Ishikawa Ankerhold","first_name":"Hellen"},{"full_name":"Hennel, Roman","last_name":"Hennel","first_name":"Roman"},{"full_name":"Benechet, Alexandre","first_name":"Alexandre","last_name":"Benechet"},{"full_name":"Lorenz, Michael","last_name":"Lorenz","first_name":"Michael"},{"last_name":"Chandraratne","first_name":"Sue","full_name":"Chandraratne, Sue"},{"first_name":"Irene","last_name":"Schubert","full_name":"Schubert, Irene"},{"first_name":"Sebastian","last_name":"Helmer","full_name":"Helmer, Sebastian"},{"last_name":"Striednig","first_name":"Bianca","full_name":"Striednig, Bianca"},{"first_name":"Konstantin","last_name":"Stark","full_name":"Stark, Konstantin"},{"last_name":"Janko","first_name":"Marek","full_name":"Janko, Marek"},{"full_name":"Böttcher, Ralph","last_name":"Böttcher","first_name":"Ralph"},{"full_name":"Verschoor, Admar","first_name":"Admar","last_name":"Verschoor"},{"first_name":"Catherine","last_name":"Leon","full_name":"Leon, Catherine"},{"full_name":"Gachet, Christian","last_name":"Gachet","first_name":"Christian"},{"first_name":"Thomas","last_name":"Gudermann","full_name":"Gudermann, Thomas"},{"last_name":"Mederos Y Schnitzler","first_name":"Michael","full_name":"Mederos Y Schnitzler, Michael"},{"last_name":"Pincus","first_name":"Zachary","full_name":"Pincus, Zachary"},{"last_name":"Iannacone","first_name":"Matteo","full_name":"Iannacone, Matteo"},{"last_name":"Haas","first_name":"Rainer","full_name":"Haas, Rainer"},{"full_name":"Wanner, Gerhard","first_name":"Gerhard","last_name":"Wanner"},{"full_name":"Lauber, Kirsten","first_name":"Kirsten","last_name":"Lauber"},{"full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Massberg, Steffen","first_name":"Steffen","last_name":"Massberg"}],"issue":"6","publication_status":"published","department":[{"_id":"MiSi"}],"date_created":"2018-12-11T11:47:15Z","title":"Migrating platelets are mechano scavengers that collect and bundle bacteria","intvolume":"       171","volume":171,"date_updated":"2021-01-12T08:03:15Z","citation":{"ieee":"F. R. Gärtner <i>et al.</i>, “Migrating platelets are mechano scavengers that collect and bundle bacteria,” <i>Cell Press</i>, vol. 171, no. 6. Cell Press, pp. 1368–1382, 2017.","chicago":"Gärtner, Florian R, Zerkah Ahmad, Gerhild Rosenberger, Shuxia Fan, Leo Nicolai, Benjamin Busch, Gökce Yavuz, et al. “Migrating Platelets Are Mechano Scavengers That Collect and Bundle Bacteria.” <i>Cell Press</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">https://doi.org/10.1016/j.cell.2017.11.001</a>.","ama":"Gärtner FR, Ahmad Z, Rosenberger G, et al. Migrating platelets are mechano scavengers that collect and bundle bacteria. <i>Cell Press</i>. 2017;171(6):1368-1382. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">10.1016/j.cell.2017.11.001</a>","apa":"Gärtner, F. R., Ahmad, Z., Rosenberger, G., Fan, S., Nicolai, L., Busch, B., … Massberg, S. (2017). Migrating platelets are mechano scavengers that collect and bundle bacteria. <i>Cell Press</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">https://doi.org/10.1016/j.cell.2017.11.001</a>","ista":"Gärtner FR, Ahmad Z, Rosenberger G, Fan S, Nicolai L, Busch B, Yavuz G, Luckner M, Ishikawa Ankerhold H, Hennel R, Benechet A, Lorenz M, Chandraratne S, Schubert I, Helmer S, Striednig B, Stark K, Janko M, Böttcher R, Verschoor A, Leon C, Gachet C, Gudermann T, Mederos Y Schnitzler M, Pincus Z, Iannacone M, Haas R, Wanner G, Lauber K, Sixt MK, Massberg S. 2017. Migrating platelets are mechano scavengers that collect and bundle bacteria. Cell Press. 171(6), 1368–1382.","mla":"Gärtner, Florian R., et al. “Migrating Platelets Are Mechano Scavengers That Collect and Bundle Bacteria.” <i>Cell Press</i>, vol. 171, no. 6, Cell Press, 2017, pp. 1368–82, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">10.1016/j.cell.2017.11.001</a>.","short":"F.R. Gärtner, Z. Ahmad, G. Rosenberger, S. Fan, L. Nicolai, B. Busch, G. Yavuz, M. Luckner, H. Ishikawa Ankerhold, R. Hennel, A. Benechet, M. Lorenz, S. Chandraratne, I. Schubert, S. Helmer, B. Striednig, K. Stark, M. Janko, R. Böttcher, A. Verschoor, C. Leon, C. Gachet, T. Gudermann, M. Mederos Y Schnitzler, Z. Pincus, M. Iannacone, R. Haas, G. Wanner, K. Lauber, M.K. Sixt, S. Massberg, Cell Press 171 (2017) 1368–1382."},"year":"2017","doi":"10.1016/j.cell.2017.11.001","day":"30","abstract":[{"text":"Blood platelets are critical for hemostasis and thrombosis and play diverse roles during immune responses. Despite these versatile tasks in mammalian biology, their skills on a cellular level are deemed limited, mainly consisting in rolling, adhesion, and aggregate formation. Here, we identify an unappreciated asset of platelets and show that adherent platelets use adhesion receptors to mechanically probe the adhesive substrate in their local microenvironment. When actomyosin-dependent traction forces overcome substrate resistance, platelets migrate and pile up the adhesive substrate together with any bound particulate material. They use this ability to act as cellular scavengers, scanning the vascular surface for potential invaders and collecting deposited bacteria. Microbe collection by migrating platelets boosts the activity of professional phagocytes, exacerbating inflammatory tissue injury in sepsis. This assigns platelets a central role in innate immune responses and identifies them as potential targets to dampen inflammatory tissue damage in clinical scenarios of severe systemic infection. In addition to their role in thrombosis and hemostasis, platelets can also migrate to sites of infection to help trap bacteria and clear the vascular surface.","lang":"eng"}],"language":[{"iso":"eng"}],"publication":"Cell Press","oa_version":"None","project":[{"grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"}],"month":"11","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","date_published":"2017-11-30T00:00:00Z","type":"journal_article","publication_identifier":{"issn":["00928674"]},"publist_id":"7243"},{"date_updated":"2021-01-12T08:05:36Z","citation":{"apa":"Engel, C., Gubbey, T., Neyer, S., Sainsbury, S., Oberthuer, C., Baejen, C., … Cramer, P. (2017). Structural basis of RNA polymerase I transcription initiation. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">https://doi.org/10.1016/j.cell.2017.03.003</a>","ama":"Engel C, Gubbey T, Neyer S, et al. Structural basis of RNA polymerase I transcription initiation. <i>Cell</i>. 2017;169(1):120-131.e22. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">10.1016/j.cell.2017.03.003</a>","chicago":"Engel, Christoph, Tobias Gubbey, Simon Neyer, Sarah Sainsbury, Christiane Oberthuer, Carlo Baejen, Carrie Bernecky, and Patrick Cramer. “Structural Basis of RNA Polymerase I Transcription Initiation.” <i>Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">https://doi.org/10.1016/j.cell.2017.03.003</a>.","ieee":"C. Engel <i>et al.</i>, “Structural basis of RNA polymerase I transcription initiation,” <i>Cell</i>, vol. 169, no. 1. Cell Press, p. 120–131.e22, 2017.","short":"C. Engel, T. Gubbey, S. Neyer, S. Sainsbury, C. Oberthuer, C. Baejen, C. Bernecky, P. Cramer, Cell 169 (2017) 120–131.e22.","mla":"Engel, Christoph, et al. “Structural Basis of RNA Polymerase I Transcription Initiation.” <i>Cell</i>, vol. 169, no. 1, Cell Press, 2017, p. 120–131.e22, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">10.1016/j.cell.2017.03.003</a>.","ista":"Engel C, Gubbey T, Neyer S, Sainsbury S, Oberthuer C, Baejen C, Bernecky C, Cramer P. 2017. Structural basis of RNA polymerase I transcription initiation. Cell. 169(1), 120–131.e22."},"year":"2017","date_published":"2017-03-23T00:00:00Z","type":"journal_article","doi":"10.1016/j.cell.2017.03.003","publication_identifier":{"issn":["00928674"]},"day":"23","abstract":[{"lang":"eng","text":"Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct “bendability” and “meltability” of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase."}],"publist_id":"7204","volume":169,"extern":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","_id":"600","publication":"Cell","author":[{"first_name":"Christoph","last_name":"Engel","full_name":"Engel, Christoph"},{"full_name":"Gubbey, Tobias","first_name":"Tobias","last_name":"Gubbey"},{"last_name":"Neyer","first_name":"Simon","full_name":"Neyer, Simon"},{"first_name":"Sarah","last_name":"Sainsbury","full_name":"Sainsbury, Sarah"},{"first_name":"Christiane","last_name":"Oberthuer","full_name":"Oberthuer, Christiane"},{"full_name":"Baejen, Carlo","last_name":"Baejen","first_name":"Carlo"},{"id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","full_name":"Bernecky, Carrie A","orcid":"0000-0003-0893-7036","last_name":"Bernecky","first_name":"Carrie A"},{"last_name":"Cramer","first_name":"Patrick","full_name":"Cramer, Patrick"}],"issue":"1","publication_status":"published","oa_version":"None","article_processing_charge":"No","date_created":"2018-12-11T11:47:25Z","title":"Structural basis of RNA polymerase I transcription initiation","month":"03","intvolume":"       169","page":"120 - 131.e22","quality_controlled":"1","language":[{"iso":"eng"}],"publisher":"Cell Press"},{"volume":156,"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","status":"public","date_updated":"2021-01-12T06:56:13Z","year":"2014","citation":{"apa":"Gadeyne, A., Sánchez Rodríguez, C., Vanneste, S., Di Rubbo, S., Zauber, H., Vanneste, K., … Van Damme, D. (2014). The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">https://doi.org/10.1016/j.cell.2014.01.039</a>","ama":"Gadeyne A, Sánchez Rodríguez C, Vanneste S, et al. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. <i>Cell</i>. 2014;156(4):691-704. doi:<a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">10.1016/j.cell.2014.01.039</a>","ieee":"A. Gadeyne <i>et al.</i>, “The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants,” <i>Cell</i>, vol. 156, no. 4. Cell Press, pp. 691–704, 2014.","chicago":"Gadeyne, Astrid, Clara Sánchez Rodríguez, Steffen Vanneste, Simone Di Rubbo, Henrik Zauber, Kevin Vanneste, Jelle Van Leene, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">https://doi.org/10.1016/j.cell.2014.01.039</a>.","mla":"Gadeyne, Astrid, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>, vol. 156, no. 4, Cell Press, 2014, pp. 691–704, doi:<a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">10.1016/j.cell.2014.01.039</a>.","short":"A. Gadeyne, C. Sánchez Rodríguez, S. Vanneste, S. Di Rubbo, H. Zauber, K. Vanneste, J. Van Leene, N. De Winne, D. Eeckhout, G. Persiau, E. Van De Slijke, B. Cannoot, L. Vercruysse, J. Mayers, M. Adamowski, U. Kania, M. Ehrlich, A. Schweighofer, T. Ketelaar, S. Maere, S. Bednarek, J. Friml, K. Gevaert, E. Witters, E. Russinova, S. Persson, G. De Jaeger, D. Van Damme, Cell 156 (2014) 691–704.","ista":"Gadeyne A, Sánchez Rodríguez C, Vanneste S, Di Rubbo S, Zauber H, Vanneste K, Van Leene J, De Winne N, Eeckhout D, Persiau G, Van De Slijke E, Cannoot B, Vercruysse L, Mayers J, Adamowski M, Kania U, Ehrlich M, Schweighofer A, Ketelaar T, Maere S, Bednarek S, Friml J, Gevaert K, Witters E, Russinova E, Persson S, De Jaeger G, Van Damme D. 2014. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. Cell. 156(4), 691–704."},"date_published":"2014-02-13T00:00:00Z","type":"journal_article","doi":"10.1016/j.cell.2014.01.039","day":"13","publication_identifier":{"issn":["00928674"]},"abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis."}],"publist_id":"4721","page":"691 - 704","quality_controlled":"1","language":[{"iso":"eng"}],"publisher":"Cell Press","_id":"2240","publication":"Cell","scopus_import":1,"author":[{"full_name":"Gadeyne, Astrid","last_name":"Gadeyne","first_name":"Astrid"},{"full_name":"Sánchez Rodríguez, Clara","last_name":"Sánchez Rodríguez","first_name":"Clara"},{"full_name":"Vanneste, Steffen","last_name":"Vanneste","first_name":"Steffen"},{"full_name":"Di Rubbo, Simone","last_name":"Di Rubbo","first_name":"Simone"},{"last_name":"Zauber","first_name":"Henrik","full_name":"Zauber, Henrik"},{"first_name":"Kevin","last_name":"Vanneste","full_name":"Vanneste, Kevin"},{"last_name":"Van Leene","first_name":"Jelle","full_name":"Van Leene, Jelle"},{"first_name":"Nancy","last_name":"De Winne","full_name":"De Winne, Nancy"},{"first_name":"Dominique","last_name":"Eeckhout","full_name":"Eeckhout, Dominique"},{"full_name":"Persiau, Geert","last_name":"Persiau","first_name":"Geert"},{"full_name":"Van De Slijke, Eveline","last_name":"Van De Slijke","first_name":"Eveline"},{"first_name":"Bernard","last_name":"Cannoot","full_name":"Cannoot, Bernard"},{"first_name":"Leen","last_name":"Vercruysse","full_name":"Vercruysse, Leen"},{"first_name":"Jonathan","last_name":"Mayers","full_name":"Mayers, Jonathan"},{"id":"45F536D2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6463-5257","full_name":"Adamowski, Maciek","first_name":"Maciek","last_name":"Adamowski"},{"first_name":"Urszula","last_name":"Kania","full_name":"Kania, Urszula","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Ehrlich, Matthias","last_name":"Ehrlich","first_name":"Matthias"},{"full_name":"Schweighofer, Alois","first_name":"Alois","last_name":"Schweighofer"},{"first_name":"Tijs","last_name":"Ketelaar","full_name":"Ketelaar, Tijs"},{"full_name":"Maere, Steven","first_name":"Steven","last_name":"Maere"},{"last_name":"Bednarek","first_name":"Sebastian","full_name":"Bednarek, Sebastian"},{"full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Kris","last_name":"Gevaert","full_name":"Gevaert, Kris"},{"last_name":"Witters","first_name":"Erwin","full_name":"Witters, Erwin"},{"full_name":"Russinova, Eugenia","first_name":"Eugenia","last_name":"Russinova"},{"first_name":"Staffan","last_name":"Persson","full_name":"Persson, Staffan"},{"full_name":"De Jaeger, Geert","first_name":"Geert","last_name":"De Jaeger"},{"full_name":"Van Damme, Daniël","last_name":"Van Damme","first_name":"Daniël"}],"issue":"4","publication_status":"published","oa_version":"None","date_created":"2018-12-11T11:56:31Z","department":[{"_id":"JiFr"}],"month":"02","title":"The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants","intvolume":"       156"}]