---
_id: '4147'
abstract:
- lang: eng
  text: We have developed a protocol to perform a genetic screen for zygotic mutations
    affecting embryogenesis on the protochordate Ciona intestinalis. The choice of
    this taxon, whose phylogenetic position places it at the basis of the chordates
    as one the most primitive vertebrate relatives, could allow to address several
    evolutionary questions. The protochordates share many morphological features with
    the vertebrates, in primis the presence of a notochord. Ciona intestinalis shows
    several ideal features for a mutational analysis, such as external development
    and larvae made of a limited number of cells and cell types. Detailed cell lineage
    studies are available. The haploid genome size is comparable to the size of the
    Drosophila haploid genome. We have optimised conditions for chemical mutagenesis
    studying the efficiency at which different concentration of N-ethyl-N-nitrosourea
    (ENU) can induce mutations. Because the adult Ciona are hermaphrodites, we are
    performing a one-generation screen. The induced mutations are identified by visual
    inspection of developmental stages. We report the preliminary results from our
    screen including examples of the different classes of mutant phenotypes found
    so far.
article_processing_charge: No
article_type: original
author:
- first_name: Paolo
  full_name: Sordino, Paolo
  last_name: Sordino
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Paola
  full_name: Cirino, Paola
  last_name: Cirino
- first_name: Alfonso
  full_name: Toscano, Alfonso
  last_name: Toscano
- first_name: Paola
  full_name: Giuliano, Paola
  last_name: Giuliano
- first_name: Rita
  full_name: Marino, Rita
  last_name: Marino
- first_name: Maria
  full_name: Pinto, Maria
  last_name: Pinto
- first_name: Rosaria
  full_name: De Santis, Rosaria
  last_name: De Santis
citation:
  ama: Sordino P, Heisenberg C-PJ, Cirino P, et al. A mutational approach to the study
    of development of the protochordate Ciona intestinalis (Tunicata, Chordata). <i>Sarsia</i>.
    2000;85(2):173-176. doi:<a href="https://doi.org/10.1080/00364827.2000.10414567">10.1080/00364827.2000.10414567</a>
  apa: Sordino, P., Heisenberg, C.-P. J., Cirino, P., Toscano, A., Giuliano, P., Marino,
    R., … De Santis, R. (2000). A mutational approach to the study of development
    of the protochordate Ciona intestinalis (Tunicata, Chordata). <i>Sarsia</i>. Taylor
    &#38; Francis. <a href="https://doi.org/10.1080/00364827.2000.10414567">https://doi.org/10.1080/00364827.2000.10414567</a>
  chicago: Sordino, Paolo, Carl-Philipp J Heisenberg, Paola Cirino, Alfonso Toscano,
    Paola Giuliano, Rita Marino, Maria Pinto, and Rosaria De Santis. “A Mutational
    Approach to the Study of Development of the Protochordate Ciona Intestinalis (Tunicata,
    Chordata).” <i>Sarsia</i>. Taylor &#38; Francis, 2000. <a href="https://doi.org/10.1080/00364827.2000.10414567">https://doi.org/10.1080/00364827.2000.10414567</a>.
  ieee: P. Sordino <i>et al.</i>, “A mutational approach to the study of development
    of the protochordate Ciona intestinalis (Tunicata, Chordata),” <i>Sarsia</i>,
    vol. 85, no. 2. Taylor &#38; Francis, pp. 173–176, 2000.
  ista: Sordino P, Heisenberg C-PJ, Cirino P, Toscano A, Giuliano P, Marino R, Pinto
    M, De Santis R. 2000. A mutational approach to the study of development of the
    protochordate Ciona intestinalis (Tunicata, Chordata). Sarsia. 85(2), 173–176.
  mla: Sordino, Paolo, et al. “A Mutational Approach to the Study of Development of
    the Protochordate Ciona Intestinalis (Tunicata, Chordata).” <i>Sarsia</i>, vol.
    85, no. 2, Taylor &#38; Francis, 2000, pp. 173–76, doi:<a href="https://doi.org/10.1080/00364827.2000.10414567">10.1080/00364827.2000.10414567</a>.
  short: P. Sordino, C.-P.J. Heisenberg, P. Cirino, A. Toscano, P. Giuliano, R. Marino,
    M. Pinto, R. De Santis, Sarsia 85 (2000) 173–176.
date_created: 2018-12-11T12:07:13Z
date_published: 2000-06-01T00:00:00Z
date_updated: 2023-04-19T14:50:58Z
day: '01'
doi: 10.1080/00364827.2000.10414567
extern: '1'
intvolume: '        85'
issue: '2'
language:
- iso: eng
month: '06'
oa_version: None
page: 173 - 176
publication: Sarsia
publication_identifier:
  issn:
  - 0036-4827
publication_status: published
publisher: Taylor & Francis
publist_id: '1972'
quality_controlled: '1'
status: public
title: A mutational approach to the study of development of the protochordate Ciona
  intestinalis (Tunicata, Chordata)
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 85
year: '2000'
...