---
_id: '669'
abstract:
- lang: eng
  text: 'The exocyst, a eukaryotic tethering complex, coregulates targeted exocytosis
    as an effector of small GTPases in polarized cell growth. In land plants, several
    exocyst subunits are encoded by double or triple paralogs, culminating in tens
    of EXO70 paralogs. Out of 23 Arabidopsis thaliana EXO70 isoforms, we analyzed
    seven isoforms expressed in pollen. Genetic and microscopic analyses of single
    mutants in EXO70A2, EXO70C1, EXO70C2, EXO70F1, EXO70H3, EXO70H5, and EXO70H6 genes
    revealed that only a loss-of-function EXO70C2 allele resulted in a significant
    male-specific transmission defect (segregation 40%:51%:9%) due to aberrant pollen
    tube growth. Mutant pollen tubes grown in vitro exhibited an enhanced growth rate
    and a decreased thickness of the tip cell wall, causing tip bursts. However, exo70C2
    pollen tubes could frequently recover and restart their speedy elongation, resulting
    in a repetitive stop-and-go growth dynamics. A pollenspecific depletion of the
    closest paralog, EXO70C1, using artificial microRNA in the exo70C2 mutant background,
    resulted in a complete pollen-specific transmission defect, suggesting redundant
    functions of EXO70C1 and EXO70C2. Both EXO70C1 and EXO70C2, GFP tagged and expressed
    under the control of their native promoters, localized in the cytoplasm of pollen
    grains, pollen tubes, and also root trichoblast cells. The expression of EXO70C2-GFP
    complemented the aberrant growth of exo70C2 pollen tubes. The absent EXO70C2 interactions
    with core exocyst subunits in the yeast two-hybrid assay, cytoplasmic localization,
    and genetic effect suggest an unconventional EXO70 function possibly as a regulator
    of exocytosis outside the exocyst complex. In conclusion, EXO70C2 is a novel factor
    contributing to the regulation of optimal tip growth of Arabidopsis pollen tubes. '
article_processing_charge: No
article_type: original
author:
- first_name: Lukáš
  full_name: Synek, Lukáš
  last_name: Synek
- first_name: Nemanja
  full_name: Vukašinović, Nemanja
  last_name: Vukašinović
- first_name: Ivan
  full_name: Kulich, Ivan
  last_name: Kulich
- first_name: Michal
  full_name: Hála, Michal
  last_name: Hála
- first_name: Klára
  full_name: Aldorfová, Klára
  last_name: Aldorfová
- first_name: Matyas
  full_name: Fendrych, Matyas
  id: 43905548-F248-11E8-B48F-1D18A9856A87
  last_name: Fendrych
  orcid: 0000-0002-9767-8699
- first_name: Viktor
  full_name: Žárský, Viktor
  last_name: Žárský
citation:
  ama: Synek L, Vukašinović N, Kulich I, et al. EXO70C2 is a key regulatory factor
    for optimal tip growth of pollen. <i>Plant Physiology</i>. 2017;174(1):223-240.
    doi:<a href="https://doi.org/10.1104/pp.16.01282">10.1104/pp.16.01282</a>
  apa: Synek, L., Vukašinović, N., Kulich, I., Hála, M., Aldorfová, K., Fendrych,
    M., &#38; Žárský, V. (2017). EXO70C2 is a key regulatory factor for optimal tip
    growth of pollen. <i>Plant Physiology</i>. American Society of Plant Biologists.
    <a href="https://doi.org/10.1104/pp.16.01282">https://doi.org/10.1104/pp.16.01282</a>
  chicago: Synek, Lukáš, Nemanja Vukašinović, Ivan Kulich, Michal Hála, Klára Aldorfová,
    Matyas Fendrych, and Viktor Žárský. “EXO70C2 Is a Key Regulatory Factor for Optimal
    Tip Growth of Pollen.” <i>Plant Physiology</i>. American Society of Plant Biologists,
    2017. <a href="https://doi.org/10.1104/pp.16.01282">https://doi.org/10.1104/pp.16.01282</a>.
  ieee: L. Synek <i>et al.</i>, “EXO70C2 is a key regulatory factor for optimal tip
    growth of pollen,” <i>Plant Physiology</i>, vol. 174, no. 1. American Society
    of Plant Biologists, pp. 223–240, 2017.
  ista: Synek L, Vukašinović N, Kulich I, Hála M, Aldorfová K, Fendrych M, Žárský
    V. 2017. EXO70C2 is a key regulatory factor for optimal tip growth of pollen.
    Plant Physiology. 174(1), 223–240.
  mla: Synek, Lukáš, et al. “EXO70C2 Is a Key Regulatory Factor for Optimal Tip Growth
    of Pollen.” <i>Plant Physiology</i>, vol. 174, no. 1, American Society of Plant
    Biologists, 2017, pp. 223–40, doi:<a href="https://doi.org/10.1104/pp.16.01282">10.1104/pp.16.01282</a>.
  short: L. Synek, N. Vukašinović, I. Kulich, M. Hála, K. Aldorfová, M. Fendrych,
    V. Žárský, Plant Physiology 174 (2017) 223–240.
date_created: 2018-12-11T11:47:49Z
date_published: 2017-05-01T00:00:00Z
date_updated: 2021-01-12T08:08:35Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1104/pp.16.01282
external_id:
  pmid:
  - '28356503'
file:
- access_level: open_access
  checksum: 97155acc6aa5f0d0a78e0589a932fe02
  content_type: application/pdf
  creator: dernst
  date_created: 2019-11-18T16:16:18Z
  date_updated: 2020-07-14T12:47:37Z
  file_id: '7041'
  file_name: 2017_PlantPhysio_Synek.pdf
  file_size: 2176903
  relation: main_file
file_date_updated: 2020-07-14T12:47:37Z
has_accepted_license: '1'
intvolume: '       174'
issue: '1'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Submitted Version
page: 223 - 240
pmid: 1
publication: Plant Physiology
publication_identifier:
  issn:
  - '00320889'
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7058'
quality_controlled: '1'
scopus_import: 1
status: public
title: EXO70C2 is a key regulatory factor for optimal tip growth of pollen
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 174
year: '2017'
...