---
_id: '799'
abstract:
- lang: eng
text: Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly
distributing various membrane proteins to their destination. Polarly localized
auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported
between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular
trafficking of PIN1 protein is sensitive to a fungal toxin brefeldin A (BFA),
which is known to inhibit guanine-nucleotide exchange factors for ADP ribosylation
factors (ARF GEFs) such as GNOM. However, the molecular details of the BFA-sensitive
trafficking pathway have not been revealed fully. In a previous study, we have
identified an Arabidopsis mutant BFA-visualized endocytic trafficking defective
3 (ben3) which exhibited reduced sensitivity to BFA in terms of BFA-induced intracellular
PIN1 agglomeration. Here, we show that BEN3 encodes a member of BIG family ARF
GEFs, BIG2. Fluorescent proteins tagged BEN3/BIG2 co-localized with markers for
TGN / early endosome (EE). Inspection of conditionally induced de novo synthesized
PIN1 confirmed that its secretion to the PM is BFA-sensitive and established BEN3/BIG2
as a crucial component of this BFA action at the level of TGN/EE. Furthermore,
ben3 mutation alleviated BFA-induced agglomeration of another TGN-localized ARF
GEF BEN1/MIN7. Taken together our results suggest that BEN3/BIG2 is an ARF GEF
component, which confers BFA sensitivity to the TGN/EE in Arabidopsis.
article_number: 1801-1811
article_processing_charge: No
author:
- first_name: Saeko
full_name: Kitakura, Saeko
last_name: Kitakura
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Yuki
full_name: Matsuura, Yuki
last_name: Matsuura
- first_name: Luca
full_name: Santuari, Luca
last_name: Santuari
- first_name: Hirotaka
full_name: Kouno, Hirotaka
last_name: Kouno
- first_name: Kohei
full_name: Arima, Kohei
last_name: Arima
- first_name: Christian
full_name: Hardtke, Christian
last_name: Hardtke
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Tatsuo
full_name: Kakimoto, Tatsuo
last_name: Kakimoto
- first_name: Hirokazu
full_name: Tanaka, Hirokazu
last_name: Tanaka
citation:
ama: Kitakura S, Adamowski M, Matsuura Y, et al. BEN3/BIG2 ARF GEF is involved in
brefeldin a-sensitive trafficking at the trans-Golgi network/early endosome in
Arabidopsis thaliana. Plant and Cell Physiology. 2017;58(10). doi:10.1093/pcp/pcx118
apa: Kitakura, S., Adamowski, M., Matsuura, Y., Santuari, L., Kouno, H., Arima,
K., … Tanaka, H. (2017). BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive
trafficking at the trans-Golgi network/early endosome in Arabidopsis thaliana.
Plant and Cell Physiology. Oxford University Press. https://doi.org/10.1093/pcp/pcx118
chicago: Kitakura, Saeko, Maciek Adamowski, Yuki Matsuura, Luca Santuari, Hirotaka
Kouno, Kohei Arima, Christian Hardtke, Jiří Friml, Tatsuo Kakimoto, and Hirokazu
Tanaka. “BEN3/BIG2 ARF GEF Is Involved in Brefeldin a-Sensitive Trafficking at
the Trans-Golgi Network/Early Endosome in Arabidopsis Thaliana.” Plant and
Cell Physiology. Oxford University Press, 2017. https://doi.org/10.1093/pcp/pcx118.
ieee: S. Kitakura et al., “BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive
trafficking at the trans-Golgi network/early endosome in Arabidopsis thaliana,”
Plant and Cell Physiology, vol. 58, no. 10. Oxford University Press, 2017.
ista: Kitakura S, Adamowski M, Matsuura Y, Santuari L, Kouno H, Arima K, Hardtke
C, Friml J, Kakimoto T, Tanaka H. 2017. BEN3/BIG2 ARF GEF is involved in brefeldin
a-sensitive trafficking at the trans-Golgi network/early endosome in Arabidopsis
thaliana. Plant and Cell Physiology. 58(10), 1801–1811.
mla: Kitakura, Saeko, et al. “BEN3/BIG2 ARF GEF Is Involved in Brefeldin a-Sensitive
Trafficking at the Trans-Golgi Network/Early Endosome in Arabidopsis Thaliana.”
Plant and Cell Physiology, vol. 58, no. 10, 1801–1811, Oxford University
Press, 2017, doi:10.1093/pcp/pcx118.
short: S. Kitakura, M. Adamowski, Y. Matsuura, L. Santuari, H. Kouno, K. Arima,
C. Hardtke, J. Friml, T. Kakimoto, H. Tanaka, Plant and Cell Physiology 58 (2017).
date_created: 2018-12-11T11:48:34Z
date_published: 2017-08-21T00:00:00Z
date_updated: 2023-09-27T11:00:19Z
day: '21'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1093/pcp/pcx118
external_id:
isi:
- '000413220400019'
pmid:
- '29016942'
file:
- access_level: open_access
checksum: bd3e3a94d55416739cbb19624bb977f8
content_type: application/pdf
creator: dernst
date_created: 2019-04-17T07:52:34Z
date_updated: 2020-07-14T12:48:06Z
file_id: '6333'
file_name: 2017_PlantCellPhysio_Kitakura.pdf
file_size: 1352913
relation: main_file
file_date_updated: 2020-07-14T12:48:06Z
has_accepted_license: '1'
intvolume: ' 58'
isi: 1
issue: '10'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Plant and Cell Physiology
publication_identifier:
issn:
- '00320781'
publication_status: published
publisher: Oxford University Press
publist_id: '6854'
pubrep_id: '1009'
quality_controlled: '1'
scopus_import: '1'
status: public
title: BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive trafficking at the trans-Golgi
network/early endosome in Arabidopsis thaliana
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 58
year: '2017'
...
---
_id: '2222'
abstract:
- lang: eng
text: Leaf venation develops complex patterns in angiosperms, but the mechanism
underlying this process is largely unknown. To elucidate the molecular mechanisms
governing vein pattern formation, we previously isolated vascular network defective
(van) mutants that displayed venation discontinuities. Here, we report the phenotypic
analysis of van4 mutants, and we identify and characterize the VAN4 gene. Detailed
phenotypic analysis shows that van4 mutants are defective in procambium cell differentiation
and subsequent vascular cell differentiation. Reduced shoot and root cell growth
is observed in van4 mutants, suggesting that VAN4 function is important for cell
growth and the establishment of venation continuity. Consistent with these phenotypes,
the VAN4 gene is strongly expressed in vascular and meristematic cells. VAN4 encodes
a putative TRS120, which is a known guanine nucleotide exchange factor (GEF) for
Rab GTPase involved in regulating vesicle transport, and a known tethering factor
that determines the specificity of membrane fusion. VAN4 protein localizes at
the trans-Golgi network/early endosome (TGN/EE). Aberrant recycling of the auxin
efflux carrier PIN proteins is observed in van4 mutants. These results suggest
that VAN4-mediated exocytosis at the TGN plays important roles in plant vascular
development and cell growth in shoot and root. Our identification of VAN4 as a
putative TRS120 shows that Rab GTPases are crucial (in addition to ARF GTPases)
for continuous vascular development, and provides further evidence for the importance
of vesicle transport in leaf vascular formation.
author:
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Tomoko
full_name: Dainobu, Tomoko
last_name: Dainobu
- first_name: Hirotomo
full_name: Takatsuka, Hirotomo
last_name: Takatsuka
- first_name: Teruyo
full_name: Okada, Teruyo
last_name: Okada
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Hiroo
full_name: Fukuda, Hiroo
last_name: Fukuda
citation:
ama: Naramoto S, Nodzyński T, Dainobu T, et al. VAN4 encodes a putative TRS120 that
is required for normal cell growth and vein development in arabidopsis. Plant
and Cell Physiology. 2014;55(4):750-763. doi:10.1093/pcp/pcu012
apa: Naramoto, S., Nodzyński, T., Dainobu, T., Takatsuka, H., Okada, T., Friml,
J., & Fukuda, H. (2014). VAN4 encodes a putative TRS120 that is required for
normal cell growth and vein development in arabidopsis. Plant and Cell Physiology.
Oxford University Press. https://doi.org/10.1093/pcp/pcu012
chicago: Naramoto, Satoshi, Tomasz Nodzyński, Tomoko Dainobu, Hirotomo Takatsuka,
Teruyo Okada, Jiří Friml, and Hiroo Fukuda. “VAN4 Encodes a Putative TRS120 That
Is Required for Normal Cell Growth and Vein Development in Arabidopsis.” Plant
and Cell Physiology. Oxford University Press, 2014. https://doi.org/10.1093/pcp/pcu012.
ieee: S. Naramoto et al., “VAN4 encodes a putative TRS120 that is required
for normal cell growth and vein development in arabidopsis,” Plant and Cell
Physiology, vol. 55, no. 4. Oxford University Press, pp. 750–763, 2014.
ista: Naramoto S, Nodzyński T, Dainobu T, Takatsuka H, Okada T, Friml J, Fukuda
H. 2014. VAN4 encodes a putative TRS120 that is required for normal cell growth
and vein development in arabidopsis. Plant and Cell Physiology. 55(4), 750–763.
mla: Naramoto, Satoshi, et al. “VAN4 Encodes a Putative TRS120 That Is Required
for Normal Cell Growth and Vein Development in Arabidopsis.” Plant and Cell
Physiology, vol. 55, no. 4, Oxford University Press, 2014, pp. 750–63, doi:10.1093/pcp/pcu012.
short: S. Naramoto, T. Nodzyński, T. Dainobu, H. Takatsuka, T. Okada, J. Friml,
H. Fukuda, Plant and Cell Physiology 55 (2014) 750–763.
date_created: 2018-12-11T11:56:24Z
date_published: 2014-04-01T00:00:00Z
date_updated: 2021-01-12T06:56:06Z
day: '01'
department:
- _id: JiFr
doi: 10.1093/pcp/pcu012
ec_funded: 1
intvolume: ' 55'
issue: '4'
language:
- iso: eng
month: '04'
oa_version: None
page: 750 - 763
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Plant and Cell Physiology
publication_identifier:
issn:
- '00320781'
publication_status: published
publisher: Oxford University Press
publist_id: '4742'
quality_controlled: '1'
scopus_import: 1
status: public
title: VAN4 encodes a putative TRS120 that is required for normal cell growth and
vein development in arabidopsis
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 55
year: '2014'
...
---
_id: '2223'
abstract:
- lang: eng
text: Correct positioning of membrane proteins is an essential process in eukaryotic
organisms. The plant hormone auxin is distributed through intercellular transport
and triggers various cellular responses. Auxin transporters of the PIN-FORMED
(PIN) family localize asymmetrically at the plasma membrane (PM) and mediate the
directional transport of auxin between cells. A fungal toxin, brefeldin A (BFA),
inhibits a subset of guanine nucleotide exchange factors for ADP-ribosylation
factor small GTPases (ARF GEFs) including GNOM, which plays a major role in localization
of PIN1 predominantly to the basal side of the PM. The Arabidopsis genome encodes
19 ARF-related putative GTPases. However, ARF components involved in PIN1 localization
have been genetically poorly defined. Using a fluorescence imaging-based forward
genetic approach, we identified an Arabidopsis mutant, bfa-visualized exocytic
trafficking defective1 (bex1), in which PM localization of PIN1-green fluorescent
protein (GFP) as well as development is hypersensitive to BFA. We found that in
bex1 a member of the ARF1 gene family, ARF1A1C, was mutated. ARF1A1C localizes
to the trans-Golgi network/early endosome and Golgi apparatus, acts synergistically
to BEN1/MIN7 ARF GEF and is important for PIN recycling to the PM. Consistent
with the developmental importance of PIN proteins, functional interference with
ARF1 resulted in an impaired auxin response gradient and various developmental
defects including embryonic patterning defects and growth arrest. Our results
show that ARF1A1C is essential for recycling of PIN auxin transporters and for
various auxin-dependent developmental processes.
author:
- first_name: Hirokazu
full_name: Tanaka, Hirokazu
last_name: Tanaka
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Saeko
full_name: Kitakura, Saeko
last_name: Kitakura
- first_name: Mugurel
full_name: Feraru, Mugurel
last_name: Feraru
- first_name: Michiko
full_name: Sasabe, Michiko
last_name: Sasabe
- first_name: Tomomi
full_name: Ishikawa, Tomomi
last_name: Ishikawa
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Tatsuo
full_name: Kakimoto, Tatsuo
last_name: Kakimoto
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tanaka H, Nodzyński T, Kitakura S, et al. BEX1/ARF1A1C is required for BFA-sensitive
recycling of PIN auxin transporters and auxin-mediated development in arabidopsis.
Plant and Cell Physiology. 2014;55(4):737-749. doi:10.1093/pcp/pct196
apa: Tanaka, H., Nodzyński, T., Kitakura, S., Feraru, M., Sasabe, M., Ishikawa,
T., … Friml, J. (2014). BEX1/ARF1A1C is required for BFA-sensitive recycling of
PIN auxin transporters and auxin-mediated development in arabidopsis. Plant
and Cell Physiology. Oxford University Press. https://doi.org/10.1093/pcp/pct196
chicago: Tanaka, Hirokazu, Tomasz Nodzyński, Saeko Kitakura, Mugurel Feraru, Michiko
Sasabe, Tomomi Ishikawa, Jürgen Kleine Vehn, Tatsuo Kakimoto, and Jiří Friml.
“BEX1/ARF1A1C Is Required for BFA-Sensitive Recycling of PIN Auxin Transporters
and Auxin-Mediated Development in Arabidopsis.” Plant and Cell Physiology.
Oxford University Press, 2014. https://doi.org/10.1093/pcp/pct196.
ieee: H. Tanaka et al., “BEX1/ARF1A1C is required for BFA-sensitive recycling
of PIN auxin transporters and auxin-mediated development in arabidopsis,” Plant
and Cell Physiology, vol. 55, no. 4. Oxford University Press, pp. 737–749,
2014.
ista: Tanaka H, Nodzyński T, Kitakura S, Feraru M, Sasabe M, Ishikawa T, Kleine
Vehn J, Kakimoto T, Friml J. 2014. BEX1/ARF1A1C is required for BFA-sensitive
recycling of PIN auxin transporters and auxin-mediated development in arabidopsis.
Plant and Cell Physiology. 55(4), 737–749.
mla: Tanaka, Hirokazu, et al. “BEX1/ARF1A1C Is Required for BFA-Sensitive Recycling
of PIN Auxin Transporters and Auxin-Mediated Development in Arabidopsis.” Plant
and Cell Physiology, vol. 55, no. 4, Oxford University Press, 2014, pp. 737–49,
doi:10.1093/pcp/pct196.
short: H. Tanaka, T. Nodzyński, S. Kitakura, M. Feraru, M. Sasabe, T. Ishikawa,
J. Kleine Vehn, T. Kakimoto, J. Friml, Plant and Cell Physiology 55 (2014) 737–749.
date_created: 2018-12-11T11:56:25Z
date_published: 2014-04-01T00:00:00Z
date_updated: 2021-01-12T06:56:07Z
day: '01'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1093/pcp/pct196
ec_funded: 1
file:
- access_level: open_access
checksum: b781a76b32ac35a520256453c3ba9433
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:25Z
date_updated: 2020-07-14T12:45:34Z
file_id: '5076'
file_name: IST-2016-431-v1+1_Plant_Cell_Physiol-2014-Tanaka-737-49.pdf
file_size: 2028111
relation: main_file
file_date_updated: 2020-07-14T12:45:34Z
has_accepted_license: '1'
intvolume: ' 55'
issue: '4'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
main_file_link:
- open_access: '1'
url: http://repository.ist.ac.at/id/eprint/431
month: '04'
oa: 1
oa_version: Published Version
page: 737 - 749
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 256BDAB0-B435-11E9-9278-68D0E5697425
name: Innovationsförderung in der Grenzregion Österreich – Tschechische Republik
durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur
publication: Plant and Cell Physiology
publication_identifier:
issn:
- '00320781'
publication_status: published
publisher: Oxford University Press
publist_id: '4741'
pubrep_id: '431'
quality_controlled: '1'
scopus_import: 1
status: public
title: BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters
and auxin-mediated development in arabidopsis
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 55
year: '2014'
...