---
_id: '10583'
abstract:
- lang: eng
  text: The synthetic strigolactone (SL) analog, rac-GR24, has been instrumental in
    studying the role of SLs as well as karrikins because it activates the receptors
    DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2) of their signaling pathways, respectively.
    Treatment with rac-GR24 modifies the root architecture at different levels, such
    as decreasing the lateral root density (LRD), while promoting root hair elongation
    or flavonol accumulation. Previously, we have shown that the flavonol biosynthesis
    is transcriptionally activated in the root by rac-GR24 treatment, but, thus far,
    the molecular players involved in that response have remained unknown. To get
    an in-depth insight into the changes that occur after the compound is perceived
    by the roots, we compared the root transcriptomes of the wild type and the more
    axillary growth2 (max2) mutant, affected in both SL and karrikin signaling pathways,
    with and without rac-GR24 treatment. Quantitative reverse transcription (qRT)-PCR,
    reporter line analysis and mutant phenotyping indicated that the flavonol response
    and the root hair elongation are controlled by the ELONGATED HYPOCOTYL 5 (HY5)
    and MYB12 transcription factors, but HY5, in contrast to MYB12, affects the LRD
    as well. Furthermore, we identified the transcription factors TARGET OF MONOPTEROS
    5 (TMO5) and TMO5 LIKE1 as negative and the Mediator complex as positive regulators
    of the rac-GR24 effect on LRD. Altogether, hereby, we get closer toward understanding
    the molecular mechanisms that underlay the rac-GR24 responses in the root.
acknowledgement: The authors thank Ralf Stracke (Bielefeld University, Bielefeld,
  Germany) for providing the myb mutants and their colleagues Bert De Rybel for the
  tmo5t;mo5l1 double mutant, Boris Parizot for tips on the RNA-seq analysis, Veronique
  Storme for statistical help on both the RNA-seq and lateral root density, and Martine
  De Cock for help in preparing the manuscript.
article_processing_charge: No
article_type: original
author:
- first_name: Sylwia
  full_name: Struk, Sylwia
  last_name: Struk
- first_name: Lukas
  full_name: Braem, Lukas
  last_name: Braem
- first_name: Cedrick
  full_name: Matthys, Cedrick
  last_name: Matthys
- first_name: Alan
  full_name: Walton, Alan
  last_name: Walton
- first_name: Nick
  full_name: Vangheluwe, Nick
  last_name: Vangheluwe
- first_name: Stan
  full_name: Van Praet, Stan
  last_name: Van Praet
- first_name: Lingxiang
  full_name: Jiang, Lingxiang
  last_name: Jiang
- first_name: Pawel
  full_name: Baster, Pawel
  id: 3028BD74-F248-11E8-B48F-1D18A9856A87
  last_name: Baster
- first_name: Carolien
  full_name: De Cuyper, Carolien
  last_name: De Cuyper
- first_name: Francois-Didier
  full_name: Boyer, Francois-Didier
  last_name: Boyer
- first_name: Elisabeth
  full_name: Stes, Elisabeth
  last_name: Stes
- first_name: Tom
  full_name: Beeckman, Tom
  last_name: Beeckman
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Kris
  full_name: Gevaert, Kris
  last_name: Gevaert
- first_name: Sofie
  full_name: Goormachtig, Sofie
  last_name: Goormachtig
citation:
  ama: Struk S, Braem L, Matthys C, et al. Transcriptional analysis in the Arabidopsis
    roots reveals new regulators that link rac-GR24 treatment with changes in flavonol
    accumulation, root hair elongation and lateral root density. <i>Plant &#38; Cell
    Physiology</i>. 2022;63(1):104-119. doi:<a href="https://doi.org/10.1093/pcp/pcab149">10.1093/pcp/pcab149</a>
  apa: Struk, S., Braem, L., Matthys, C., Walton, A., Vangheluwe, N., Van Praet, S.,
    … Goormachtig, S. (2022). Transcriptional analysis in the Arabidopsis roots reveals
    new regulators that link rac-GR24 treatment with changes in flavonol accumulation,
    root hair elongation and lateral root density. <i>Plant &#38; Cell Physiology</i>.
    Oxford University Press. <a href="https://doi.org/10.1093/pcp/pcab149">https://doi.org/10.1093/pcp/pcab149</a>
  chicago: Struk, Sylwia, Lukas Braem, Cedrick Matthys, Alan Walton, Nick Vangheluwe,
    Stan Van Praet, Lingxiang Jiang, et al. “Transcriptional Analysis in the Arabidopsis
    Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol
    Accumulation, Root Hair Elongation and Lateral Root Density.” <i>Plant &#38; Cell
    Physiology</i>. Oxford University Press, 2022. <a href="https://doi.org/10.1093/pcp/pcab149">https://doi.org/10.1093/pcp/pcab149</a>.
  ieee: S. Struk <i>et al.</i>, “Transcriptional analysis in the Arabidopsis roots
    reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation,
    root hair elongation and lateral root density,” <i>Plant &#38; Cell Physiology</i>,
    vol. 63, no. 1. Oxford University Press, pp. 104–119, 2022.
  ista: Struk S, Braem L, Matthys C, Walton A, Vangheluwe N, Van Praet S, Jiang L,
    Baster P, De Cuyper C, Boyer F-D, Stes E, Beeckman T, Friml J, Gevaert K, Goormachtig
    S. 2022. Transcriptional analysis in the Arabidopsis roots reveals new regulators
    that link rac-GR24 treatment with changes in flavonol accumulation, root hair
    elongation and lateral root density. Plant &#38; Cell Physiology. 63(1), 104–119.
  mla: Struk, Sylwia, et al. “Transcriptional Analysis in the Arabidopsis Roots Reveals
    New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation,
    Root Hair Elongation and Lateral Root Density.” <i>Plant &#38; Cell Physiology</i>,
    vol. 63, no. 1, Oxford University Press, 2022, pp. 104–19, doi:<a href="https://doi.org/10.1093/pcp/pcab149">10.1093/pcp/pcab149</a>.
  short: S. Struk, L. Braem, C. Matthys, A. Walton, N. Vangheluwe, S. Van Praet, L.
    Jiang, P. Baster, C. De Cuyper, F.-D. Boyer, E. Stes, T. Beeckman, J. Friml, K.
    Gevaert, S. Goormachtig, Plant &#38; Cell Physiology 63 (2022) 104–119.
date_created: 2021-12-28T11:44:18Z
date_published: 2022-01-21T00:00:00Z
date_updated: 2023-08-02T13:40:43Z
day: '21'
department:
- _id: JiFr
doi: 10.1093/pcp/pcab149
external_id:
  isi:
  - '000877899400009'
  pmid:
  - '34791413'
intvolume: '        63'
isi: 1
issue: '1'
keyword:
- flavonols
- MAX2
- rac-Gr24
- RNA-seq
- root development
- transcriptional regulation
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1093/pcp/pcab149
month: '01'
oa: 1
oa_version: Published Version
page: 104-119
pmid: 1
publication: Plant & Cell Physiology
publication_identifier:
  eissn:
  - 1471-9053
  issn:
  - 0032-0781
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Transcriptional analysis in the Arabidopsis roots reveals new regulators that
  link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation
  and lateral root density
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 63
year: '2022'
...
---
_id: '6104'
abstract:
- lang: eng
  text: Abiotic stress poses constant challenges for plant survival and is a serious
    problem for global agricultural productivity. On a molecular level, stress conditions
    result in elevation of reactive oxygen species (ROS) production causing oxidative
    stress associated with oxidation of proteins and nucleic acids as well as impairment
    of membrane functions. Adaptation of root growth to ROS accumulation is facilitated
    through modification of auxin and cytokinin hormone homeostasis. Here, we report
    that in Arabidopsis root meristem, ROS-induced changes of auxin levels correspond
    to decreased abundance of PIN auxin efflux carriers at the plasma membrane (PM).
    Specifically, increase in H2O2 levels affects PIN2 endocytic recycling. We show
    that the PIN2 intracellular trafficking during adaptation to oxidative stress
    requires the function of the ADP-ribosylation factor (ARF)-guanine-nucleotide
    exchange factor (GEF) BEN1, an actin-associated regulator of the trafficking from
    the PM to early endosomes and, presumably, indirectly, trafficking to the vacuoles.
    We propose that H2O2 levels affect the actin dynamics thus modulating ARF-GEF-dependent
    trafficking of PIN2. This mechanism provides a way how root growth acclimates
    to stress and adapts to a changing environment.
article_processing_charge: No
author:
- first_name: Marta
  full_name: Zwiewka, Marta
  last_name: Zwiewka
- first_name: Agnieszka
  full_name: Bielach, Agnieszka
  last_name: Bielach
- first_name: Prashanth
  full_name: Tamizhselvan, Prashanth
  last_name: Tamizhselvan
- first_name: Sharmila
  full_name: Madhavan, Sharmila
  last_name: Madhavan
- first_name: Eman Elrefaay
  full_name: Ryad, Eman Elrefaay
  last_name: Ryad
- first_name: Shutang
  full_name: Tan, Shutang
  id: 2DE75584-F248-11E8-B48F-1D18A9856A87
  last_name: Tan
  orcid: 0000-0002-0471-8285
- first_name: Mónika
  full_name: Hrtyan, Mónika
  id: 45A71A74-F248-11E8-B48F-1D18A9856A87
  last_name: Hrtyan
- first_name: Petre
  full_name: Dobrev, Petre
  last_name: Dobrev
- first_name: Radomira
  full_name: Vanková, Radomira
  last_name: Vanková
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Vanesa B.
  full_name: Tognetti, Vanesa B.
  last_name: Tognetti
citation:
  ama: Zwiewka M, Bielach A, Tamizhselvan P, et al. Root adaptation to H2O2-induced
    oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking.
    <i>Plant and Cell Physiology</i>. 2019;60(2):255-273. doi:<a href="https://doi.org/10.1093/pcp/pcz001">10.1093/pcp/pcz001</a>
  apa: Zwiewka, M., Bielach, A., Tamizhselvan, P., Madhavan, S., Ryad, E. E., Tan,
    S., … Tognetti, V. B. (2019). Root adaptation to H2O2-induced oxidative stress
    by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking. <i>Plant and Cell
    Physiology</i>. Oxford University Press. <a href="https://doi.org/10.1093/pcp/pcz001">https://doi.org/10.1093/pcp/pcz001</a>
  chicago: Zwiewka, Marta, Agnieszka Bielach, Prashanth Tamizhselvan, Sharmila Madhavan,
    Eman Elrefaay Ryad, Shutang Tan, Mónika Hrtyan, et al. “Root Adaptation to H2O2-Induced
    Oxidative Stress by ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking.”
    <i>Plant and Cell Physiology</i>. Oxford University Press, 2019. <a href="https://doi.org/10.1093/pcp/pcz001">https://doi.org/10.1093/pcp/pcz001</a>.
  ieee: M. Zwiewka <i>et al.</i>, “Root adaptation to H2O2-induced oxidative stress
    by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking,” <i>Plant and Cell
    Physiology</i>, vol. 60, no. 2. Oxford University Press, pp. 255–273, 2019.
  ista: Zwiewka M, Bielach A, Tamizhselvan P, Madhavan S, Ryad EE, Tan S, Hrtyan M,
    Dobrev P, Vanková R, Friml J, Tognetti VB. 2019. Root adaptation to H2O2-induced
    oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking.
    Plant and Cell Physiology. 60(2), 255–273.
  mla: Zwiewka, Marta, et al. “Root Adaptation to H2O2-Induced Oxidative Stress by
    ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking.” <i>Plant and Cell Physiology</i>,
    vol. 60, no. 2, Oxford University Press, 2019, pp. 255–73, doi:<a href="https://doi.org/10.1093/pcp/pcz001">10.1093/pcp/pcz001</a>.
  short: M. Zwiewka, A. Bielach, P. Tamizhselvan, S. Madhavan, E.E. Ryad, S. Tan,
    M. Hrtyan, P. Dobrev, R. Vanková, J. Friml, V.B. Tognetti, Plant and Cell Physiology
    60 (2019) 255–273.
date_created: 2019-03-17T22:59:14Z
date_published: 2019-02-01T00:00:00Z
date_updated: 2023-08-25T08:05:28Z
day: '01'
department:
- _id: JiFr
doi: 10.1093/pcp/pcz001
external_id:
  isi:
  - '000459634300002'
  pmid:
  - '30668780'
intvolume: '        60'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 255-273
pmid: 1
publication: Plant and Cell Physiology
publication_identifier:
  eissn:
  - 1471-9053
  issn:
  - 0032-0781
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated
  PIN2 trafficking
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 60
year: '2019'
...
---
_id: '12196'
abstract:
- lang: eng
  text: SNC1 (SUPPRESSOR OF NPR1, CONSTITUTIVE 1) is one of a suite of intracellular
    Arabidopsis NOD-like receptor (NLR) proteins which, upon activation, result in
    the induction of defense responses. However, the molecular mechanisms underlying
    NLR activation and the subsequent provocation of immune responses are only partially
    characterized. To identify negative regulators of NLR-mediated immunity, a forward
    genetic screen was undertaken to search for enhancers of the dwarf, autoimmune
    gain-of-function snc1 mutant. To avoid lethality resulting from severe dwarfism,
    the screen was conducted using mos4 (modifier of snc1, 4) snc1 plants, which display
    wild-type-like morphology and resistance. M2 progeny were screened for mutant,
    snc1-enhancing (muse) mutants displaying a reversion to snc1-like phenotypes.
    The muse9 mos4 snc1 triple mutant was found to exhibit dwarf morphology, elevated
    expression of the pPR2-GUS defense marker reporter gene and enhanced resistance
    to the oomycete pathogen Hyaloperonospora arabidopsidis Noco2. Via map-based cloning
    and Illumina sequencing, it was determined that the muse9 mutation is in the gene
    encoding the SWI/SNF chromatin remodeler SYD (SPLAYED), and was thus renamed syd-10.
    The syd-10 single mutant has no observable alteration from wild-type-like resistance,
    although the syd-4 T-DNA insertion allele displays enhanced resistance to the
    bacterial pathogen Pseudomonas syringae pv. maculicola ES4326. Transcription of
    SNC1 is increased in both syd-4 and syd-10. These data suggest that SYD plays
    a subtle, specific role in the regulation of SNC1 expression and SNC1-mediated
    immunity. SYD may work with other proteins at the chromatin level to repress SNC1
    transcription; such regulation is important for fine-tuning the expression of
    NLR-encoding genes to prevent unpropitious autoimmunity.
acknowledgement: "This work was supported by the National Sciences and Engineering
  Research Council of Canada [Canada Graduate\r\nScholarship–Doctoral to K.J.; Discovery
  Grant to X.L.]; the department of Botany at the University of f British Columbia\r\n[the
  Dewar Cooper Memorial Fund to X.L.].The authors would like to thank Dr. Yuelin Zhang
  and Ms. Yan Li for their assistance with next-generation sequencing, and Mr. Charles
  Copeland for critical reading of the manuscript."
article_processing_charge: No
article_type: original
author:
- first_name: Kaeli C.M.
  full_name: Johnson, Kaeli C.M.
  last_name: Johnson
- first_name: Shitou
  full_name: Xia, Shitou
  last_name: Xia
- first_name: Xiaoqi
  full_name: Feng, Xiaoqi
  id: e0164712-22ee-11ed-b12a-d80fcdf35958
  last_name: Feng
  orcid: 0000-0002-4008-1234
- first_name: Xin
  full_name: Li, Xin
  last_name: Li
citation:
  ama: Johnson KCM, Xia S, Feng X, Li X. The chromatin remodeler SPLAYED negatively
    regulates SNC1-mediated immunity. <i>Plant and Cell Physiology</i>. 2015;56(8):1616-1623.
    doi:<a href="https://doi.org/10.1093/pcp/pcv087">10.1093/pcp/pcv087</a>
  apa: Johnson, K. C. M., Xia, S., Feng, X., &#38; Li, X. (2015). The chromatin remodeler
    SPLAYED negatively regulates SNC1-mediated immunity. <i>Plant and Cell Physiology</i>.
    Oxford University Press. <a href="https://doi.org/10.1093/pcp/pcv087">https://doi.org/10.1093/pcp/pcv087</a>
  chicago: Johnson, Kaeli C.M., Shitou Xia, Xiaoqi Feng, and Xin Li. “The Chromatin
    Remodeler SPLAYED Negatively Regulates SNC1-Mediated Immunity.” <i>Plant and Cell
    Physiology</i>. Oxford University Press, 2015. <a href="https://doi.org/10.1093/pcp/pcv087">https://doi.org/10.1093/pcp/pcv087</a>.
  ieee: K. C. M. Johnson, S. Xia, X. Feng, and X. Li, “The chromatin remodeler SPLAYED
    negatively regulates SNC1-mediated immunity,” <i>Plant and Cell Physiology</i>,
    vol. 56, no. 8. Oxford University Press, pp. 1616–1623, 2015.
  ista: Johnson KCM, Xia S, Feng X, Li X. 2015. The chromatin remodeler SPLAYED negatively
    regulates SNC1-mediated immunity. Plant and Cell Physiology. 56(8), 1616–1623.
  mla: Johnson, Kaeli C. M., et al. “The Chromatin Remodeler SPLAYED Negatively Regulates
    SNC1-Mediated Immunity.” <i>Plant and Cell Physiology</i>, vol. 56, no. 8, Oxford
    University Press, 2015, pp. 1616–23, doi:<a href="https://doi.org/10.1093/pcp/pcv087">10.1093/pcp/pcv087</a>.
  short: K.C.M. Johnson, S. Xia, X. Feng, X. Li, Plant and Cell Physiology 56 (2015)
    1616–1623.
date_created: 2023-01-16T09:20:22Z
date_published: 2015-08-01T00:00:00Z
date_updated: 2023-05-08T11:03:23Z
department:
- _id: XiFe
doi: 10.1093/pcp/pcv087
extern: '1'
external_id:
  pmid:
  - '26063389'
intvolume: '        56'
issue: '8'
keyword:
- Cell Biology
- Plant Science
- Physiology
- General Medicine
language:
- iso: eng
month: '08'
oa_version: None
page: 1616-1623
pmid: 1
publication: Plant and Cell Physiology
publication_identifier:
  issn:
  - 0032-0781
  - 1471-9053
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: The chromatin remodeler SPLAYED negatively regulates SNC1-mediated immunity
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 56
year: '2015'
...