---
_id: '3497'
abstract:
- lang: eng
text: The use of advanced patch-clamp recording techniques in brain slices, such
as simultaneous recording from multiple neurons and recording from dendrites or
presynaptic terminals, demands slices of the highest quality. In this context
the mechanics of the tissue slicer are an important factor. Ideally, a tissue
slicer should generate large-amplitude and high-frequency movements of the cutting
blade in a horizontal axis, with minimal vibrations in the vertical axis. We developed
a vibroslicer that fulfils these in part conflicting requirements. The oscillator
is a permanent-magnet-coil-leaf-spring system. Using an auto-resonant mechano-electrical
feedback circuit, large horizontal oscillations (up to 3 mm peak-to-peak) with
high frequency (,90 Hz) are generated. To minimize vertical vibrations, an adjustment
mechanism was employed that allowed alignment of the cutting edge of the blade
with the major axis of the oscillation. A vibroprobe device was used to monitor
vertical vibrations during adjustment. The system is based on the shading of the
light path between a light-emitting diode (LED) and a photodiode. Vibroprobe monitoring
revealed that the vibroslicer, after appropriate adjustment, generated vertical
vibrations of <1 µm, significantly less than many commercial tissue slicers.
Light- and electron-microscopic analysis of surface layers of slices cut with
the vibroslicer showed that cellular elements, dendritic processes and presynaptic
terminals are well preserved under these conditions, as required for patch-clamp
recording from these structures.
acknowledgement: "We thank Dr. M. Frotscher for reading the manuscript, and H. Kressner,
R. Laufersweiler, and A. Bühler for help with the construction of several prototypes
of vibroslicer and vibroprobe. We also thank A. Blomenkamp, K. Winterhalter, B.
Joch, and A. Schneider for technical assistance. This work was supported by grants
of the Deutsche Forschungsgemeinschaft\r\n(SFB 505/C5, C6) and the Human Frontiers
Science Program Organization (RG0017/1998-B)."
article_processing_charge: No
article_type: original
author:
- first_name: Jörg
full_name: Geiger, Jörg
last_name: Geiger
- first_name: Joseph
full_name: Bischofberger, Joseph
last_name: Bischofberger
- first_name: Imre
full_name: Vida, Imre
last_name: Vida
- first_name: Ulrich
full_name: Fröbe, Ulrich
last_name: Fröbe
- first_name: S
full_name: Pfitzinger, S
last_name: Pfitzinger
- first_name: H.
full_name: Weber, H.
last_name: Weber
- first_name: Klaus
full_name: Haverkampf, Klaus
last_name: Haverkampf
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Geiger J, Bischofberger J, Vida I, et al. Patch-clamp recording in brain slices
with improved slicer technology. Pflugers Archiv : European Journal of Physiology.
2002;443(3):491-501. doi:10.1007/s00424-001-0735-3'
apa: 'Geiger, J., Bischofberger, J., Vida, I., Fröbe, U., Pfitzinger, S., Weber,
H., … Jonas, P. M. (2002). Patch-clamp recording in brain slices with improved
slicer technology. Pflugers Archiv : European Journal of Physiology. Springer.
https://doi.org/10.1007/s00424-001-0735-3'
chicago: 'Geiger, Jörg, Joseph Bischofberger, Imre Vida, Ulrich Fröbe, S Pfitzinger,
H. Weber, Klaus Haverkampf, and Peter M Jonas. “Patch-Clamp Recording in Brain
Slices with Improved Slicer Technology.” Pflugers Archiv : European Journal
of Physiology. Springer, 2002. https://doi.org/10.1007/s00424-001-0735-3.'
ieee: 'J. Geiger et al., “Patch-clamp recording in brain slices with improved
slicer technology,” Pflugers Archiv : European Journal of Physiology, vol.
443, no. 3. Springer, pp. 491–501, 2002.'
ista: 'Geiger J, Bischofberger J, Vida I, Fröbe U, Pfitzinger S, Weber H, Haverkampf
K, Jonas PM. 2002. Patch-clamp recording in brain slices with improved slicer
technology. Pflugers Archiv : European Journal of Physiology. 443(3), 491–501.'
mla: 'Geiger, Jörg, et al. “Patch-Clamp Recording in Brain Slices with Improved
Slicer Technology.” Pflugers Archiv : European Journal of Physiology, vol.
443, no. 3, Springer, 2002, pp. 491–501, doi:10.1007/s00424-001-0735-3.'
short: 'J. Geiger, J. Bischofberger, I. Vida, U. Fröbe, S. Pfitzinger, H. Weber,
K. Haverkampf, P.M. Jonas, Pflugers Archiv : European Journal of Physiology 443
(2002) 491–501.'
date_created: 2018-12-11T12:03:38Z
date_published: 2002-01-01T00:00:00Z
date_updated: 2023-07-17T07:36:37Z
day: '01'
doi: 10.1007/s00424-001-0735-3
extern: '1'
external_id:
pmid:
- '11810221'
intvolume: ' 443'
issue: '3'
language:
- iso: eng
month: '01'
oa_version: None
page: 491 - 501
pmid: 1
publication: 'Pflugers Archiv : European Journal of Physiology'
publication_identifier:
issn:
- 0031-6768
publication_status: published
publisher: Springer
publist_id: '2890'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Patch-clamp recording in brain slices with improved slicer technology
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 443
year: '2002'
...
---
_id: '3468'
abstract:
- lang: eng
text: "Two types of metabolically regulated K channels have been identified for
the first time in enzymatically demyelinated fibres of amphibian sciatic nerve
using the patch-clamp technique. A maxi K channel with a single-channel conductance
of 132 pS (105 mM K on both sides of the membrane, 15°C) is activated both by
micromolar concentrations of internal Ca and by depolarization. A second type
of K channel with a conductance of 44 pS is inhibited by intracellular adenosine
5'-triphosphate (ATP) with a half-maximal inhibitory concentration (IC50) of 35
μM. It is blocked by submicromolar concentrations of external glibenclamide. Both
channels are sensitive to external tetraethylammonium chloride (IC50 = 0.2 mM
for the maxi K channel and 4.2 mM for the ATP-sensitive channel). They may be
part of a complex feedback system regulating axonal excitability under various
metabolic conditions.\r\n"
article_processing_charge: No
article_type: original
author:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Duk
full_name: Koh, Duk
last_name: Koh
- first_name: Knut
full_name: Kampe, Knut
last_name: Kampe
- first_name: Markus
full_name: Hermsteiner, Markus
last_name: Hermsteiner
- first_name: Werner
full_name: Vogel, Werner
last_name: Vogel
citation:
ama: 'Jonas PM, Koh D, Kampe K, Hermsteiner M, Vogel W. ATP-sensitive and Ca-activated
K channels in vertebrate axons: novel links between metabolism and excitability.
Pflügers Archiv : European Journal of Physiology. 1991;418(1-2):68-73.
doi:10.1007/BF00370453'
apa: 'Jonas, P. M., Koh, D., Kampe, K., Hermsteiner, M., & Vogel, W. (1991).
ATP-sensitive and Ca-activated K channels in vertebrate axons: novel links between
metabolism and excitability. Pflügers Archiv : European Journal of Physiology.
Springer. https://doi.org/10.1007/BF00370453'
chicago: 'Jonas, Peter M, Duk Koh, Knut Kampe, Markus Hermsteiner, and Werner Vogel.
“ATP-Sensitive and Ca-Activated K Channels in Vertebrate Axons: Novel Links between
Metabolism and Excitability.” Pflügers Archiv : European Journal of Physiology.
Springer, 1991. https://doi.org/10.1007/BF00370453.'
ieee: 'P. M. Jonas, D. Koh, K. Kampe, M. Hermsteiner, and W. Vogel, “ATP-sensitive
and Ca-activated K channels in vertebrate axons: novel links between metabolism
and excitability,” Pflügers Archiv : European Journal of Physiology, vol.
418, no. 1–2. Springer, pp. 68–73, 1991.'
ista: 'Jonas PM, Koh D, Kampe K, Hermsteiner M, Vogel W. 1991. ATP-sensitive and
Ca-activated K channels in vertebrate axons: novel links between metabolism and
excitability. Pflügers Archiv : European Journal of Physiology. 418(1–2), 68–73.'
mla: 'Jonas, Peter M., et al. “ATP-Sensitive and Ca-Activated K Channels in Vertebrate
Axons: Novel Links between Metabolism and Excitability.” Pflügers Archiv :
European Journal of Physiology, vol. 418, no. 1–2, Springer, 1991, pp. 68–73,
doi:10.1007/BF00370453.'
short: 'P.M. Jonas, D. Koh, K. Kampe, M. Hermsteiner, W. Vogel, Pflügers Archiv :
European Journal of Physiology 418 (1991) 68–73.'
date_created: 2018-12-11T12:03:29Z
date_published: 1991-01-01T00:00:00Z
date_updated: 2022-03-03T14:42:06Z
day: '01'
doi: 10.1007/BF00370453
extern: '1'
external_id:
pmid:
- '2041727'
intvolume: ' 418'
issue: 1-2
language:
- iso: eng
main_file_link:
- url: https://link.springer.com/article/10.1007/BF00370453
month: '01'
oa_version: None
page: 68 - 73
pmid: 1
publication: 'Pflügers Archiv : European Journal of Physiology'
publication_identifier:
eissn:
- 1432-2013
issn:
- 0031-6768
publication_status: published
publisher: Springer
publist_id: '2919'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'ATP-sensitive and Ca-activated K channels in vertebrate axons: novel links
between metabolism and excitability'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 418
year: '1991'
...
---
_id: '3464'
abstract:
- lang: eng
text: The effects of the major neurotoxic fraction isolated from scorpion venom
of Tityus serrulatus, TiTx gamma, on peripheral nerve membrane of Xenopus laevis
were studied under current- and voltage-clamp conditions. 700 nmol/l TiTx gamma
depolarized the membrane and induced spontaneous activity (150 s-1, maximum value),
which ceased within a few minutes. It reduced the amplitude of the action potentials
from 109 mV to 52 mV and increased their duration from 1.25 ms to 4.5 ms. 440
nmol/l TiTx gamma induced inward Na current flow at resting potential. The descending
branch of the Na current-voltage curve was flattened and shifted approximately
10 mV to more negative potentials. Maximum Na permeability was reduced to about
20%. Both development of and recovery from inactivation of Na permeability were
slowed. The steepness of the steady-state inactivation curve was decreased, but
the mid-potential changed only insignificantly. No prepulse was necessary to elicit
either a shift of activation or an inward current at resting potential. Expressing
the toxin effect either in terms of the decrease of Na peak current or of the
slowing of inactivation, half-maximum effects were found with 0.3 +/- 0.1 and
3.7 +/- 0.7 mumol/l TiTx gamma, respectively.
article_processing_charge: No
article_type: original
author:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Werner
full_name: Vogel, Werner
last_name: Vogel
- first_name: Eliane
full_name: Arantes, Eliane
last_name: Arantes
- first_name: Jose
full_name: Giglio, Jose
last_name: Giglio
citation:
ama: 'Jonas PM, Vogel W, Arantes E, Giglio J. Toxin γ of the scorpion Tityus serrulatus
modifies both activation and inactivation of sodium permeability of nerve membrane.
Pflugers Archiv : European Journal of Physiology. 1986;407(1):92-99. doi:10.1007/BF00580727'
apa: 'Jonas, P. M., Vogel, W., Arantes, E., & Giglio, J. (1986). Toxin γ of
the scorpion Tityus serrulatus modifies both activation and inactivation of sodium
permeability of nerve membrane. Pflugers Archiv : European Journal of Physiology.
Springer. https://doi.org/10.1007/BF00580727'
chicago: 'Jonas, Peter M, Werner Vogel, Eliane Arantes, and Jose Giglio. “Toxin
γ of the Scorpion Tityus Serrulatus Modifies Both Activation and Inactivation
of Sodium Permeability of Nerve Membrane.” Pflugers Archiv : European Journal
of Physiology. Springer, 1986. https://doi.org/10.1007/BF00580727.'
ieee: 'P. M. Jonas, W. Vogel, E. Arantes, and J. Giglio, “Toxin γ of the scorpion
Tityus serrulatus modifies both activation and inactivation of sodium permeability
of nerve membrane,” Pflugers Archiv : European Journal of Physiology, vol.
407, no. 1. Springer, pp. 92–99, 1986.'
ista: 'Jonas PM, Vogel W, Arantes E, Giglio J. 1986. Toxin γ of the scorpion Tityus
serrulatus modifies both activation and inactivation of sodium permeability of
nerve membrane. Pflugers Archiv : European Journal of Physiology. 407(1), 92–99.'
mla: 'Jonas, Peter M., et al. “Toxin γ of the Scorpion Tityus Serrulatus Modifies
Both Activation and Inactivation of Sodium Permeability of Nerve Membrane.” Pflugers
Archiv : European Journal of Physiology, vol. 407, no. 1, Springer, 1986,
pp. 92–99, doi:10.1007/BF00580727.'
short: 'P.M. Jonas, W. Vogel, E. Arantes, J. Giglio, Pflugers Archiv : European
Journal of Physiology 407 (1986) 92–99.'
date_created: 2018-12-11T12:03:28Z
date_published: 1986-07-01T00:00:00Z
date_updated: 2022-02-03T09:46:42Z
day: '01'
doi: 10.1007/BF00580727
extern: '1'
external_id:
pmid:
- '2426654 '
intvolume: ' 407'
issue: '1'
language:
- iso: eng
month: '07'
oa_version: None
page: 92 - 99
pmid: 1
publication: 'Pflugers Archiv : European Journal of Physiology'
publication_identifier:
eissn:
- 1432-2013
issn:
- 0031-6768
publication_status: published
publisher: Springer
publist_id: '2923'
quality_controlled: '1'
status: public
title: Toxin γ of the scorpion Tityus serrulatus modifies both activation and inactivation
of sodium permeability of nerve membrane
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 407
year: '1986'
...