[{"page":"491 - 501","quality_controlled":"1","publisher":"Springer","article_type":"original","_id":"3497","pmid":1,"scopus_import":"1","author":[{"first_name":"Jörg","last_name":"Geiger","full_name":"Geiger, Jörg"},{"last_name":"Bischofberger","first_name":"Joseph","full_name":"Bischofberger, Joseph"},{"last_name":"Vida","first_name":"Imre","full_name":"Vida, Imre"},{"full_name":"Fröbe, Ulrich","last_name":"Fröbe","first_name":"Ulrich"},{"full_name":"Pfitzinger, S","first_name":"S","last_name":"Pfitzinger"},{"first_name":"H.","last_name":"Weber","full_name":"Weber, H."},{"last_name":"Haverkampf","first_name":"Klaus","full_name":"Haverkampf, Klaus"},{"id":"353C1B58-F248-11E8-B48F-1D18A9856A87","first_name":"Peter M","last_name":"Jonas","orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M"}],"issue":"3","publication_status":"published","date_created":"2018-12-11T12:03:38Z","article_processing_charge":"No","title":"Patch-clamp recording in brain slices with improved slicer technology","intvolume":"       443","acknowledgement":"We thank Dr. M. Frotscher for reading the manuscript, and H. Kressner, R. Laufersweiler, and A. Bühler for help with the construction of several prototypes of vibroslicer and vibroprobe. We also thank A. Blomenkamp, K. Winterhalter, B. Joch, and A. Schneider for technical assistance. This work was supported by grants of the Deutsche Forschungsgemeinschaft\r\n(SFB 505/C5, C6) and the Human Frontiers Science Program Organization (RG0017/1998-B).","volume":443,"extern":"1","date_updated":"2023-07-17T07:36:37Z","year":"2002","citation":{"ista":"Geiger J, Bischofberger J, Vida I, Fröbe U, Pfitzinger S, Weber H, Haverkampf K, Jonas PM. 2002. Patch-clamp recording in brain slices with improved slicer technology. Pflugers Archiv : European Journal of Physiology. 443(3), 491–501.","short":"J. Geiger, J. Bischofberger, I. Vida, U. Fröbe, S. Pfitzinger, H. Weber, K. Haverkampf, P.M. Jonas, Pflugers Archiv : European Journal of Physiology 443 (2002) 491–501.","mla":"Geiger, Jörg, et al. “Patch-Clamp Recording in Brain Slices with Improved Slicer Technology.” <i>Pflugers Archiv : European Journal of Physiology</i>, vol. 443, no. 3, Springer, 2002, pp. 491–501, doi:<a href=\"https://doi.org/10.1007/s00424-001-0735-3\">10.1007/s00424-001-0735-3</a>.","chicago":"Geiger, Jörg, Joseph Bischofberger, Imre Vida, Ulrich Fröbe, S Pfitzinger, H. Weber, Klaus Haverkampf, and Peter M Jonas. “Patch-Clamp Recording in Brain Slices with Improved Slicer Technology.” <i>Pflugers Archiv : European Journal of Physiology</i>. Springer, 2002. <a href=\"https://doi.org/10.1007/s00424-001-0735-3\">https://doi.org/10.1007/s00424-001-0735-3</a>.","ieee":"J. Geiger <i>et al.</i>, “Patch-clamp recording in brain slices with improved slicer technology,” <i>Pflugers Archiv : European Journal of Physiology</i>, vol. 443, no. 3. Springer, pp. 491–501, 2002.","ama":"Geiger J, Bischofberger J, Vida I, et al. Patch-clamp recording in brain slices with improved slicer technology. <i>Pflugers Archiv : European Journal of Physiology</i>. 2002;443(3):491-501. doi:<a href=\"https://doi.org/10.1007/s00424-001-0735-3\">10.1007/s00424-001-0735-3</a>","apa":"Geiger, J., Bischofberger, J., Vida, I., Fröbe, U., Pfitzinger, S., Weber, H., … Jonas, P. M. (2002). Patch-clamp recording in brain slices with improved slicer technology. <i>Pflugers Archiv : European Journal of Physiology</i>. Springer. <a href=\"https://doi.org/10.1007/s00424-001-0735-3\">https://doi.org/10.1007/s00424-001-0735-3</a>"},"external_id":{"pmid":["11810221"]},"doi":"10.1007/s00424-001-0735-3","day":"01","abstract":[{"lang":"eng","text":"The use of advanced patch-clamp recording techniques in brain slices, such as simultaneous recording from multiple neurons and recording from dendrites or presynaptic terminals, demands slices of the highest quality. In this context the mechanics of the tissue slicer are an important factor. Ideally, a tissue slicer should generate large-amplitude and high-frequency movements of the cutting blade in a horizontal axis, with minimal vibrations in the vertical axis. We developed a vibroslicer that fulfils these in part conflicting requirements. The oscillator is a permanent-magnet-coil-leaf-spring system. Using an auto-resonant mechano-electrical feedback circuit, large horizontal oscillations (up to 3 mm peak-to-peak) with high frequency (,90 Hz) are generated. To minimize vertical vibrations, an adjustment mechanism was employed that allowed alignment of the cutting edge of the blade with the major axis of the oscillation. A vibroprobe device was used to monitor vertical vibrations during adjustment. The system is based on the shading of the light path between a light-emitting diode (LED) and a photodiode. Vibroprobe monitoring revealed that the vibroslicer, after appropriate adjustment, generated vertical vibrations of &lt;1 µm, significantly less than many commercial tissue slicers. Light- and electron-microscopic analysis of surface layers of slices cut with the vibroslicer showed that cellular elements, dendritic processes and presynaptic terminals are well preserved under these conditions, as required for patch-clamp recording from these structures."}],"language":[{"iso":"eng"}],"publication":"Pflugers Archiv : European Journal of Physiology","oa_version":"None","month":"01","status":"public","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_published":"2002-01-01T00:00:00Z","type":"journal_article","publication_identifier":{"issn":["0031-6768"]},"publist_id":"2890"},{"main_file_link":[{"url":"https://link.springer.com/article/10.1007/BF00370453"}],"status":"public","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_published":"1991-01-01T00:00:00Z","type":"journal_article","publication_identifier":{"eissn":["1432-2013"],"issn":["0031-6768"]},"publist_id":"2919","language":[{"iso":"eng"}],"publication":"Pflügers Archiv : European Journal of Physiology","oa_version":"None","month":"01","volume":418,"extern":"1","date_updated":"2022-03-03T14:42:06Z","year":"1991","citation":{"chicago":"Jonas, Peter M, Duk Koh, Knut Kampe, Markus Hermsteiner, and Werner Vogel. “ATP-Sensitive and Ca-Activated K Channels in Vertebrate Axons: Novel Links between Metabolism and Excitability.” <i>Pflügers Archiv : European Journal of Physiology</i>. Springer, 1991. <a href=\"https://doi.org/10.1007/BF00370453\">https://doi.org/10.1007/BF00370453</a>.","ieee":"P. M. Jonas, D. Koh, K. Kampe, M. Hermsteiner, and W. Vogel, “ATP-sensitive and Ca-activated K channels in vertebrate axons: novel links between metabolism and excitability,” <i>Pflügers Archiv : European Journal of Physiology</i>, vol. 418, no. 1–2. Springer, pp. 68–73, 1991.","ama":"Jonas PM, Koh D, Kampe K, Hermsteiner M, Vogel W. ATP-sensitive and Ca-activated K channels in vertebrate axons: novel links between metabolism and excitability. <i>Pflügers Archiv : European Journal of Physiology</i>. 1991;418(1-2):68-73. doi:<a href=\"https://doi.org/10.1007/BF00370453\">10.1007/BF00370453</a>","apa":"Jonas, P. M., Koh, D., Kampe, K., Hermsteiner, M., &#38; Vogel, W. (1991). ATP-sensitive and Ca-activated K channels in vertebrate axons: novel links between metabolism and excitability. <i>Pflügers Archiv : European Journal of Physiology</i>. Springer. <a href=\"https://doi.org/10.1007/BF00370453\">https://doi.org/10.1007/BF00370453</a>","ista":"Jonas PM, Koh D, Kampe K, Hermsteiner M, Vogel W. 1991. ATP-sensitive and Ca-activated K channels in vertebrate axons: novel links between metabolism and excitability. Pflügers Archiv : European Journal of Physiology. 418(1–2), 68–73.","mla":"Jonas, Peter M., et al. “ATP-Sensitive and Ca-Activated K Channels in Vertebrate Axons: Novel Links between Metabolism and Excitability.” <i>Pflügers Archiv : European Journal of Physiology</i>, vol. 418, no. 1–2, Springer, 1991, pp. 68–73, doi:<a href=\"https://doi.org/10.1007/BF00370453\">10.1007/BF00370453</a>.","short":"P.M. Jonas, D. Koh, K. Kampe, M. Hermsteiner, W. Vogel, Pflügers Archiv : European Journal of Physiology 418 (1991) 68–73."},"external_id":{"pmid":["2041727"]},"doi":"10.1007/BF00370453","day":"01","abstract":[{"lang":"eng","text":"Two types of metabolically regulated K channels have been identified for the first time in enzymatically demyelinated fibres of amphibian sciatic nerve using the patch-clamp technique. A maxi K channel with a single-channel conductance of 132 pS (105 mM K on both sides of the membrane, 15°C) is activated both by micromolar concentrations of internal Ca and by depolarization. A second type of K channel with a conductance of 44 pS is inhibited by intracellular adenosine 5'-triphosphate (ATP) with a half-maximal inhibitory concentration (IC50) of 35 μM. It is blocked by submicromolar concentrations of external glibenclamide. Both channels are sensitive to external tetraethylammonium chloride (IC50 = 0.2 mM for the maxi K channel and 4.2 mM for the ATP-sensitive channel). They may be part of a complex feedback system regulating axonal excitability under various metabolic conditions.\r\n"}],"page":"68 - 73","quality_controlled":"1","publisher":"Springer","article_type":"original","_id":"3468","pmid":1,"scopus_import":"1","author":[{"id":"353C1B58-F248-11E8-B48F-1D18A9856A87","first_name":"Peter M","last_name":"Jonas","orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M"},{"full_name":"Koh, Duk","last_name":"Koh","first_name":"Duk"},{"last_name":"Kampe","first_name":"Knut","full_name":"Kampe, Knut"},{"full_name":"Hermsteiner, Markus","last_name":"Hermsteiner","first_name":"Markus"},{"last_name":"Vogel","first_name":"Werner","full_name":"Vogel, Werner"}],"issue":"1-2","publication_status":"published","article_processing_charge":"No","date_created":"2018-12-11T12:03:29Z","title":"ATP-sensitive and Ca-activated K channels in vertebrate axons: novel links between metabolism and excitability","intvolume":"       418"},{"language":[{"iso":"eng"}],"oa_version":"None","month":"07","publication":"Pflugers Archiv : European Journal of Physiology","status":"public","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publication_identifier":{"eissn":["1432-2013"],"issn":["0031-6768"]},"publist_id":"2923","type":"journal_article","date_published":"1986-07-01T00:00:00Z","publisher":"Springer","article_type":"original","quality_controlled":"1","page":"92 - 99","date_created":"2018-12-11T12:03:28Z","article_processing_charge":"No","publication_status":"published","intvolume":"       407","title":"Toxin γ of the scorpion Tityus serrulatus modifies both activation and inactivation of sodium permeability of nerve membrane","_id":"3464","pmid":1,"issue":"1","author":[{"id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804","last_name":"Jonas","first_name":"Peter M"},{"full_name":"Vogel, Werner","first_name":"Werner","last_name":"Vogel"},{"last_name":"Arantes","first_name":"Eliane","full_name":"Arantes, Eliane"},{"first_name":"Jose","last_name":"Giglio","full_name":"Giglio, Jose"}],"volume":407,"extern":"1","day":"01","doi":"10.1007/BF00580727","abstract":[{"text":"The effects of the major neurotoxic fraction isolated from scorpion venom of Tityus serrulatus, TiTx gamma, on peripheral nerve membrane of Xenopus laevis were studied under current- and voltage-clamp conditions. 700 nmol/l TiTx gamma depolarized the membrane and induced spontaneous activity (150 s-1, maximum value), which ceased within a few minutes. It reduced the amplitude of the action potentials from 109 mV to 52 mV and increased their duration from 1.25 ms to 4.5 ms. 440 nmol/l TiTx gamma induced inward Na current flow at resting potential. The descending branch of the Na current-voltage curve was flattened and shifted approximately 10 mV to more negative potentials. Maximum Na permeability was reduced to about 20%. Both development of and recovery from inactivation of Na permeability were slowed. The steepness of the steady-state inactivation curve was decreased, but the mid-potential changed only insignificantly. No prepulse was necessary to elicit either a shift of activation or an inward current at resting potential. Expressing the toxin effect either in terms of the decrease of Na peak current or of the slowing of inactivation, half-maximum effects were found with 0.3 +/- 0.1 and 3.7 +/- 0.7 mumol/l TiTx gamma, respectively.","lang":"eng"}],"year":"1986","citation":{"chicago":"Jonas, Peter M, Werner Vogel, Eliane Arantes, and Jose Giglio. “Toxin γ of the Scorpion Tityus Serrulatus Modifies Both Activation and Inactivation of Sodium Permeability of Nerve Membrane.” <i>Pflugers Archiv : European Journal of Physiology</i>. Springer, 1986. <a href=\"https://doi.org/10.1007/BF00580727\">https://doi.org/10.1007/BF00580727</a>.","ieee":"P. M. Jonas, W. Vogel, E. Arantes, and J. Giglio, “Toxin γ of the scorpion Tityus serrulatus modifies both activation and inactivation of sodium permeability of nerve membrane,” <i>Pflugers Archiv : European Journal of Physiology</i>, vol. 407, no. 1. Springer, pp. 92–99, 1986.","apa":"Jonas, P. M., Vogel, W., Arantes, E., &#38; Giglio, J. (1986). Toxin γ of the scorpion Tityus serrulatus modifies both activation and inactivation of sodium permeability of nerve membrane. <i>Pflugers Archiv : European Journal of Physiology</i>. Springer. <a href=\"https://doi.org/10.1007/BF00580727\">https://doi.org/10.1007/BF00580727</a>","ama":"Jonas PM, Vogel W, Arantes E, Giglio J. Toxin γ of the scorpion Tityus serrulatus modifies both activation and inactivation of sodium permeability of nerve membrane. <i>Pflugers Archiv : European Journal of Physiology</i>. 1986;407(1):92-99. doi:<a href=\"https://doi.org/10.1007/BF00580727\">10.1007/BF00580727</a>","ista":"Jonas PM, Vogel W, Arantes E, Giglio J. 1986. Toxin γ of the scorpion Tityus serrulatus modifies both activation and inactivation of sodium permeability of nerve membrane. Pflugers Archiv : European Journal of Physiology. 407(1), 92–99.","mla":"Jonas, Peter M., et al. “Toxin γ of the Scorpion Tityus Serrulatus Modifies Both Activation and Inactivation of Sodium Permeability of Nerve Membrane.” <i>Pflugers Archiv : European Journal of Physiology</i>, vol. 407, no. 1, Springer, 1986, pp. 92–99, doi:<a href=\"https://doi.org/10.1007/BF00580727\">10.1007/BF00580727</a>.","short":"P.M. Jonas, W. Vogel, E. Arantes, J. Giglio, Pflugers Archiv : European Journal of Physiology 407 (1986) 92–99."},"date_updated":"2022-02-03T09:46:42Z","external_id":{"pmid":["2426654 "]}}]