---
_id: '13266'
abstract:
- lang: eng
text: The 3′,5′-cyclic adenosine monophosphate (cAMP) is a versatile second messenger
in many mammalian signaling pathways. However, its role in plants remains not
well-recognized. Recent discovery of adenylate cyclase (AC) activity for transport
inhibitor response 1/auxin-signaling F-box proteins (TIR1/AFB) auxin receptors
and the demonstration of its importance for canonical auxin signaling put plant
cAMP research back into spotlight. This insight briefly summarizes the well-established
cAMP signaling pathways in mammalian cells and describes the turbulent and controversial
history of plant cAMP research highlighting the major progress and the unresolved
points. We also briefly review the current paradigm of auxin signaling to provide
a background for the discussion on the AC activity of TIR1/AFB auxin receptors
and its potential role in transcriptional auxin signaling as well as impact of
these discoveries on plant cAMP research in general.
acknowledgement: 'We gratefully acknowledge our brave colleagues, whose excellent
efforts kept the plant cAMP research going in the last two decades. The authors
were financially supported by the Austrian Science Fund (FWF): I 6123 and P 37051-B.'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Qi L, Friml J. Tale of cAMP as a second messenger in auxin signaling and beyond.
New Phytologist. 2023;240(2):489-495. doi:10.1111/nph.19123
apa: Qi, L., & Friml, J. (2023). Tale of cAMP as a second messenger in auxin
signaling and beyond. New Phytologist. Wiley. https://doi.org/10.1111/nph.19123
chicago: Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin
Signaling and Beyond.” New Phytologist. Wiley, 2023. https://doi.org/10.1111/nph.19123.
ieee: L. Qi and J. Friml, “Tale of cAMP as a second messenger in auxin signaling
and beyond,” New Phytologist, vol. 240, no. 2. Wiley, pp. 489–495, 2023.
ista: Qi L, Friml J. 2023. Tale of cAMP as a second messenger in auxin signaling
and beyond. New Phytologist. 240(2), 489–495.
mla: Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin Signaling
and Beyond.” New Phytologist, vol. 240, no. 2, Wiley, 2023, pp. 489–95,
doi:10.1111/nph.19123.
short: L. Qi, J. Friml, New Phytologist 240 (2023) 489–495.
date_created: 2023-07-23T22:01:13Z
date_published: 2023-10-01T00:00:00Z
date_updated: 2024-01-29T11:21:55Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.19123
external_id:
isi:
- '001026321500001'
pmid:
- '37434303'
file:
- access_level: open_access
checksum: 6d9bbd45b8e7bb3ceee2586d447bacb2
content_type: application/pdf
creator: dernst
date_created: 2024-01-29T11:21:43Z
date_updated: 2024-01-29T11:21:43Z
file_id: '14898'
file_name: 2023_NewPhytologist_Qi.pdf
file_size: 974464
relation: main_file
success: 1
file_date_updated: 2024-01-29T11:21:43Z
has_accepted_license: '1'
intvolume: ' 240'
isi: 1
issue: '2'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 489-495
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptor complexes for auxin canalization and regeneration in Arabidopsis
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tale of cAMP as a second messenger in auxin signaling and beyond
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 240
year: '2023'
...
---
_id: '10282'
abstract:
- lang: eng
text: Advanced transcriptome sequencing has revealed that the majority of eukaryotic
genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated
to investigating the functional relevance of particular splicing events, even
those in the key developmental and hormonal regulators. Combining approaches of
genetics, biochemistry and advanced confocal microscopy, we describe the impact
of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana.
PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces
two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing
in a four amino acid stretch, exhibit almost identical expression patterns and
subcellular localization. We reveal that they are closely associated and mutually
influence each other's mobility within the plasma membrane. Phenotypic complementation
tests indicate that the functional contribution of PIN7b per se is minor, but
it markedly reduces the prominent PIN7a activity, which is required for correct
seedling apical hook formation and auxin-mediated tropic responses. Our results
establish alternative splicing of the PIN family as a conserved, functionally
relevant mechanism, revealing an additional regulatory level of auxin-mediated
plant development.
acknowledgement: We thank Claus Schwechheimer for the pin34 and pin347 seeds, Yuliia
Mironova for technical assistance, Ksenia Timofeyenko and Dmitry Konovalov for help
with the evolutional analysis, Konstantin Kutashev and Siarhei Dabravolski for assistance
with FRET-FLIM, Huibin Han for advice with hypocotyl imaging, Karel Müller for the
initial qRT-PCR on the tobacco cell lines, Stano Pekár for suggestions regarding
the statistical analysis of the morphodynamic measurements, and Jozef Mravec, Dolf
Weijers and Lindy Abas for their comments on the manuscript. This work was supported
by the Czech Science Foundation (projects 16-26428S and 19-23773S to IK, MH and
KRůžička, 19-18917S to JHumpolíčková and 18-26981S to JF), and the Ministry of Education,
Youth and Sports of the Czech Republic (MEYS, CZ.02.1.01/0.0/0.0/16_019/0000738)
to KRůžička and JHejátko. The imaging facilities of the Institute of Experimental
Botany and CEITEC are supported by MEYS (LM2018129 – Czech BioImaging and CZ.02.1.01/0.0/0.0/16_013/0001775).
The authors declare no competing interests.
article_processing_charge: No
article_type: original
author:
- first_name: Ivan
full_name: Kashkan, Ivan
last_name: Kashkan
- first_name: Mónika
full_name: Hrtyan, Mónika
id: 45A71A74-F248-11E8-B48F-1D18A9856A87
last_name: Hrtyan
- first_name: Katarzyna
full_name: Retzer, Katarzyna
last_name: Retzer
- first_name: Jana
full_name: Humpolíčková, Jana
last_name: Humpolíčková
- first_name: Aswathy
full_name: Jayasree, Aswathy
last_name: Jayasree
- first_name: Roberta
full_name: Filepová, Roberta
last_name: Filepová
- first_name: Zuzana
full_name: Vondráková, Zuzana
last_name: Vondráková
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Debbie
full_name: Rombaut, Debbie
last_name: Rombaut
- first_name: Thomas B.
full_name: Jacobs, Thomas B.
last_name: Jacobs
- first_name: Mikko J.
full_name: Frilander, Mikko J.
last_name: Frilander
- first_name: Jan
full_name: Hejátko, Jan
last_name: Hejátko
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Kamil
full_name: Růžička, Kamil
last_name: Růžička
citation:
ama: Kashkan I, Hrtyan M, Retzer K, et al. Mutually opposing activity of PIN7 splicing
isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana.
New Phytologist. 2021;233:329-343. doi:10.1111/nph.17792
apa: Kashkan, I., Hrtyan, M., Retzer, K., Humpolíčková, J., Jayasree, A., Filepová,
R., … Růžička, K. (2021). Mutually opposing activity of PIN7 splicing isoforms
is required for auxin-mediated tropic responses in Arabidopsis thaliana. New
Phytologist. Wiley. https://doi.org/10.1111/nph.17792
chicago: Kashkan, Ivan, Mónika Hrtyan, Katarzyna Retzer, Jana Humpolíčková, Aswathy
Jayasree, Roberta Filepová, Zuzana Vondráková, et al. “Mutually Opposing Activity
of PIN7 Splicing Isoforms Is Required for Auxin-Mediated Tropic Responses in Arabidopsis
Thaliana.” New Phytologist. Wiley, 2021. https://doi.org/10.1111/nph.17792.
ieee: I. Kashkan et al., “Mutually opposing activity of PIN7 splicing isoforms
is required for auxin-mediated tropic responses in Arabidopsis thaliana,” New
Phytologist, vol. 233. Wiley, pp. 329–343, 2021.
ista: Kashkan I, Hrtyan M, Retzer K, Humpolíčková J, Jayasree A, Filepová R, Vondráková
Z, Simon S, Rombaut D, Jacobs TB, Frilander MJ, Hejátko J, Friml J, Petrášek J,
Růžička K. 2021. Mutually opposing activity of PIN7 splicing isoforms is required
for auxin-mediated tropic responses in Arabidopsis thaliana. New Phytologist.
233, 329–343.
mla: Kashkan, Ivan, et al. “Mutually Opposing Activity of PIN7 Splicing Isoforms
Is Required for Auxin-Mediated Tropic Responses in Arabidopsis Thaliana.” New
Phytologist, vol. 233, Wiley, 2021, pp. 329–43, doi:10.1111/nph.17792.
short: I. Kashkan, M. Hrtyan, K. Retzer, J. Humpolíčková, A. Jayasree, R. Filepová,
Z. Vondráková, S. Simon, D. Rombaut, T.B. Jacobs, M.J. Frilander, J. Hejátko,
J. Friml, J. Petrášek, K. Růžička, New Phytologist 233 (2021) 329–343.
date_created: 2021-11-14T23:01:24Z
date_published: 2021-11-05T00:00:00Z
date_updated: 2023-08-14T11:46:43Z
day: '05'
department:
- _id: JiFr
doi: 10.1111/nph.17792
external_id:
isi:
- '000714678100001'
pmid:
- '34637542'
intvolume: ' 233'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.biorxiv.org/content/10.1101/2020.05.02.074070v2
month: '11'
oa: 1
oa_version: Preprint
page: 329-343
pmid: 1
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated
tropic responses in Arabidopsis thaliana
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 233
year: '2021'
...
---
_id: '7697'
abstract:
- lang: eng
text: "* Morphogenesis and adaptive tropic growth in plants depend on gradients
of the phytohormone auxin, mediated by the membrane‐based PIN‐FORMED (PIN) auxin
transporters. PINs localize to a particular side of the plasma membrane (PM) or
to the endoplasmic reticulum (ER) to directionally transport auxin and maintain
intercellular and intracellular auxin homeostasis, respectively. However, the
molecular cues that confer their diverse cellular localizations remain largely
unknown.\r\n* In this study, we systematically swapped the domains between ER‐
and PM‐localized PIN proteins, as well as between apical and basal PM‐localized
PINs from Arabidopsis thaliana , to shed light on why PIN family members with
similar topological structures reside at different membrane compartments within
cells.\r\n* Our results show that not only do the N‐ and C‐terminal transmembrane
domains (TMDs) and central hydrophilic loop contribute to their differential subcellular
localizations and cellular polarity, but that the pairwise‐matched N‐ and C‐terminal
TMDs resulting from intramolecular domain–domain coevolution are also crucial
for their divergent patterns of localization.\r\n* These findings illustrate the
complexity of the evolutionary path of PIN proteins in acquiring their plethora
of developmental functions and adaptive growth in plants."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Corinna
full_name: Hartinger, Corinna
id: AEFB2266-8ABF-11EA-AA39-812C3623CBE4
last_name: Hartinger
orcid: 0000-0003-1618-2737
- first_name: Xiaojuan
full_name: Wang, Xiaojuan
last_name: Wang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Hartinger C, Wang X, Friml J. Directional auxin fluxes in plants by
intramolecular domain‐domain co‐evolution of PIN auxin transporters. New Phytologist.
2020;227(5):1406-1416. doi:10.1111/nph.16629
apa: Zhang, Y., Hartinger, C., Wang, X., & Friml, J. (2020). Directional auxin
fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters.
New Phytologist. Wiley. https://doi.org/10.1111/nph.16629
chicago: Zhang, Yuzhou, Corinna Hartinger, Xiaojuan Wang, and Jiří Friml. “Directional
Auxin Fluxes in Plants by Intramolecular Domain‐domain Co‐evolution of PIN Auxin
Transporters.” New Phytologist. Wiley, 2020. https://doi.org/10.1111/nph.16629.
ieee: Y. Zhang, C. Hartinger, X. Wang, and J. Friml, “Directional auxin fluxes in
plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters,”
New Phytologist, vol. 227, no. 5. Wiley, pp. 1406–1416, 2020.
ista: Zhang Y, Hartinger C, Wang X, Friml J. 2020. Directional auxin fluxes in plants
by intramolecular domain‐domain co‐evolution of PIN auxin transporters. New Phytologist.
227(5), 1406–1416.
mla: Zhang, Yuzhou, et al. “Directional Auxin Fluxes in Plants by Intramolecular
Domain‐domain Co‐evolution of PIN Auxin Transporters.” New Phytologist,
vol. 227, no. 5, Wiley, 2020, pp. 1406–16, doi:10.1111/nph.16629.
short: Y. Zhang, C. Hartinger, X. Wang, J. Friml, New Phytologist 227 (2020) 1406–1416.
date_created: 2020-04-30T08:43:29Z
date_published: 2020-09-01T00:00:00Z
date_updated: 2023-09-05T15:46:04Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.16629
ec_funded: 1
external_id:
isi:
- '000534092400001'
pmid:
- '32350870'
file:
- access_level: open_access
checksum: 8e8150dbbba8cb65b72f81d1f0864b8b
content_type: application/pdf
creator: dernst
date_created: 2020-11-24T12:19:38Z
date_updated: 2020-11-24T12:19:38Z
file_id: '8799'
file_name: 2020_09_NewPhytologist_Zhang.pdf
file_size: 3643395
relation: main_file
success: 1
file_date_updated: 2020-11-24T12:19:38Z
has_accepted_license: '1'
intvolume: ' 227'
isi: 1
issue: '5'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 1406-1416
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution
of PIN auxin transporters
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 227
year: '2020'
...
---
_id: '6856'
abstract:
- lang: eng
text: 'Plant mating systems play a key role in structuring genetic variation both
within and between species. In hybrid zones, the outcomes and dynamics of hybridization
are usually interpreted as the balance between gene flow and selection against
hybrids. Yet, mating systems can introduce selective forces that alter these expectations;
with diverse outcomes for the level and direction of gene flow depending on variation
in outcrossing and whether the mating systems of the species pair are the same
or divergent. We present a survey of hybridization in 133 species pairs from 41
plant families and examine how patterns of hybridization vary with mating system.
We examine if hybrid zone mode, level of gene flow, asymmetries in gene flow and
the frequency of reproductive isolating barriers vary in relation to mating system/s
of the species pair. We combine these results with a simulation model and examples
from the literature to address two general themes: (i) the two‐way interaction
between introgression and the evolution of reproductive systems, and (ii) how
mating system can facilitate or restrict interspecific gene flow. We conclude
that examining mating system with hybridization provides unique opportunities
to understand divergence and the processes underlying reproductive isolation.'
article_processing_charge: No
article_type: original
author:
- first_name: Melinda
full_name: Pickup, Melinda
id: 2C78037E-F248-11E8-B48F-1D18A9856A87
last_name: Pickup
orcid: 0000-0001-6118-0541
- first_name: Nicholas H
full_name: Barton, Nicholas H
id: 4880FE40-F248-11E8-B48F-1D18A9856A87
last_name: Barton
orcid: 0000-0002-8548-5240
- first_name: Yaniv
full_name: Brandvain, Yaniv
last_name: Brandvain
- first_name: Christelle
full_name: Fraisse, Christelle
id: 32DF5794-F248-11E8-B48F-1D18A9856A87
last_name: Fraisse
orcid: 0000-0001-8441-5075
- first_name: Sarah
full_name: Yakimowski, Sarah
last_name: Yakimowski
- first_name: Tanmay
full_name: Dixit, Tanmay
last_name: Dixit
- first_name: Christian
full_name: Lexer, Christian
last_name: Lexer
- first_name: Eva
full_name: Cereghetti, Eva
id: 71AA91B4-05ED-11EA-8BEB-F5833E63BD63
last_name: Cereghetti
- first_name: David
full_name: Field, David
id: 419049E2-F248-11E8-B48F-1D18A9856A87
last_name: Field
orcid: 0000-0002-4014-8478
citation:
ama: 'Pickup M, Barton NH, Brandvain Y, et al. Mating system variation in hybrid
zones: Facilitation, barriers and asymmetries to gene flow. New Phytologist.
2019;224(3):1035-1047. doi:10.1111/nph.16180'
apa: 'Pickup, M., Barton, N. H., Brandvain, Y., Fraisse, C., Yakimowski, S., Dixit,
T., … Field, D. (2019). Mating system variation in hybrid zones: Facilitation,
barriers and asymmetries to gene flow. New Phytologist. Wiley. https://doi.org/10.1111/nph.16180'
chicago: 'Pickup, Melinda, Nicholas H Barton, Yaniv Brandvain, Christelle Fraisse,
Sarah Yakimowski, Tanmay Dixit, Christian Lexer, Eva Cereghetti, and David Field.
“Mating System Variation in Hybrid Zones: Facilitation, Barriers and Asymmetries
to Gene Flow.” New Phytologist. Wiley, 2019. https://doi.org/10.1111/nph.16180.'
ieee: 'M. Pickup et al., “Mating system variation in hybrid zones: Facilitation,
barriers and asymmetries to gene flow,” New Phytologist, vol. 224, no.
3. Wiley, pp. 1035–1047, 2019.'
ista: 'Pickup M, Barton NH, Brandvain Y, Fraisse C, Yakimowski S, Dixit T, Lexer
C, Cereghetti E, Field D. 2019. Mating system variation in hybrid zones: Facilitation,
barriers and asymmetries to gene flow. New Phytologist. 224(3), 1035–1047.'
mla: 'Pickup, Melinda, et al. “Mating System Variation in Hybrid Zones: Facilitation,
Barriers and Asymmetries to Gene Flow.” New Phytologist, vol. 224, no.
3, Wiley, 2019, pp. 1035–47, doi:10.1111/nph.16180.'
short: M. Pickup, N.H. Barton, Y. Brandvain, C. Fraisse, S. Yakimowski, T. Dixit,
C. Lexer, E. Cereghetti, D. Field, New Phytologist 224 (2019) 1035–1047.
date_created: 2019-09-07T14:35:40Z
date_published: 2019-11-01T00:00:00Z
date_updated: 2023-10-18T08:47:08Z
day: '01'
ddc:
- '570'
department:
- _id: NiBa
doi: 10.1111/nph.16180
ec_funded: 1
external_id:
pmid:
- '31505037'
file:
- access_level: open_access
checksum: 21e4c95599bbcaf7c483b89954658672
content_type: application/pdf
creator: dernst
date_created: 2019-11-13T08:15:05Z
date_updated: 2020-07-14T12:47:42Z
file_id: '7011'
file_name: 2019_NewPhytologist_Pickup.pdf
file_size: 1511958
relation: main_file
file_date_updated: 2020-07-14T12:47:42Z
has_accepted_license: '1'
intvolume: ' 224'
issue: '3'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 1035-1047
pmid: 1
project:
- _id: 25B36484-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '329960'
name: Mating system and the evolutionary dynamics of hybrid zones
- _id: 2662AADE-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: M02463
name: Sex chromosomes and species barriers
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Mating system variation in hybrid zones: Facilitation, barriers and asymmetries
to gene flow'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 224
year: '2019'
...