[{"date_published":"1999-10-01T00:00:00Z","publisher":"Elsevier","volume":38,"quality_controlled":"1","extern":"1","page":"1631 - 1640","abstract":[{"lang":"eng","text":"Presynaptic metabotropic glutamate receptors (mGluRs) of group III constitute possible targets for putative neuroprotective drugs acting against glutamate excitotoxic insults. Indeed, in glutamatergic cerebellar granule neurones in culture, high concentrations of L-2-amino-4-phosphonobutyrate (L-AP4, above 0.3 mM, thus activating mGluR7) inhibit NMDA-induced cell death. In contrast, in striatal cultures which are enriched in GABAergic neurones, we show that high concentrations of L-AP4 increased neuronal death in control as well as in NMDA-stimulated cultures. Moreover, similar results were obtained with the GABA(B)R agonist, baclofen. Both the neuroprotective effects in cerebellar granule cells and the neurotoxic effects in striatal neurones were mediated via Gi-Go-coupled mGluRs, suggesting that these effects were probably mediated by mGluR7a or b and GABA(B)R expressed in these neurones. In striatal neurones, we found that L-AP4 and baclofen inhibited both basal and NMDA-stimulated GABA release. These inhibitions of GABA release may be responsible for the increase in basal and NMDA-stimulated neuronal death. Indeed, blockade of GABA(A) receptors with bicuculline increased neuronal death of control and NMDA-treated striatal cultures. Taken together, these results suggest that L-AP4 and baclofen, via mGluR7 and GABA(B)R, reduced the neuroprotective effect of GABA present in striatal cultures acting via GABA(A) receptors. Although caution must be taken when extrapolating from in vitro to in vivo situations, the present experiments and the recent observations that mGluR7 and GABA(B)R are expressed in heterologous synapses, should be taken into consideration when evaluating the neuroprotective action of future mGluR7 specific agonists or GABA(B)R specific antagonists."}],"external_id":{"pmid":["10530824 "]},"publication_status":"published","doi":"10.1016/S0028-3908(99)00124-0","_id":"2595","article_type":"original","year":"1999","acknowledgement":"We thank A. Turner-Madeuf for English revision, M. Passama and L. Charvet for the illustrations, Isabelle Brabet, Cécile Joly and Jaroslav Blahos for helpful technical assistance. This work was supported by the CNRS, Bayer \r\nFrance/Troponwerke (Germany), and CEE-Biomed BMH4-2 CT 960228.","citation":{"apa":"Lafon Cazal, M., Viennois, G., Kühn, R., Malitschek, B., Pin, J., Shigemoto, R., &#38; Bockaërt, J. (1999). mGluR7-like receptor and GABA(B) receptor activation enhance neurotoxic effects of N-methyl-D-aspartate in cultured mouse striatal GABAergic neurones. <i>Neuropharmacology</i>. Elsevier. <a href=\"https://doi.org/10.1016/S0028-3908(99)00124-0\">https://doi.org/10.1016/S0028-3908(99)00124-0</a>","ieee":"M. Lafon Cazal <i>et al.</i>, “mGluR7-like receptor and GABA(B) receptor activation enhance neurotoxic effects of N-methyl-D-aspartate in cultured mouse striatal GABAergic neurones,” <i>Neuropharmacology</i>, vol. 38, no. 10. Elsevier, pp. 1631–1640, 1999.","ama":"Lafon Cazal M, Viennois G, Kühn R, et al. mGluR7-like receptor and GABA(B) receptor activation enhance neurotoxic effects of N-methyl-D-aspartate in cultured mouse striatal GABAergic neurones. <i>Neuropharmacology</i>. 1999;38(10):1631-1640. doi:<a href=\"https://doi.org/10.1016/S0028-3908(99)00124-0\">10.1016/S0028-3908(99)00124-0</a>","short":"M. Lafon Cazal, G. Viennois, R. Kühn, B. Malitschek, J. Pin, R. Shigemoto, J. Bockaërt, Neuropharmacology 38 (1999) 1631–1640.","chicago":"Lafon Cazal, Mireille, Gaëlle Viennois, Rainer Kühn, Barbara Malitschek, Jean Pin, Ryuichi Shigemoto, and Joël Bockaërt. “MGluR7-like Receptor and GABA(B) Receptor Activation Enhance Neurotoxic Effects of N-Methyl-D-Aspartate in Cultured Mouse Striatal GABAergic Neurones.” <i>Neuropharmacology</i>. Elsevier, 1999. <a href=\"https://doi.org/10.1016/S0028-3908(99)00124-0\">https://doi.org/10.1016/S0028-3908(99)00124-0</a>.","ista":"Lafon Cazal M, Viennois G, Kühn R, Malitschek B, Pin J, Shigemoto R, Bockaërt J. 1999. mGluR7-like receptor and GABA(B) receptor activation enhance neurotoxic effects of N-methyl-D-aspartate in cultured mouse striatal GABAergic neurones. Neuropharmacology. 38(10), 1631–1640.","mla":"Lafon Cazal, Mireille, et al. “MGluR7-like Receptor and GABA(B) Receptor Activation Enhance Neurotoxic Effects of N-Methyl-D-Aspartate in Cultured Mouse Striatal GABAergic Neurones.” <i>Neuropharmacology</i>, vol. 38, no. 10, Elsevier, 1999, pp. 1631–40, doi:<a href=\"https://doi.org/10.1016/S0028-3908(99)00124-0\">10.1016/S0028-3908(99)00124-0</a>."},"publist_id":"4302","date_created":"2018-12-11T11:58:34Z","status":"public","type":"journal_article","pmid":1,"month":"10","publication_identifier":{"issn":["0028-3908"]},"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","oa_version":"None","day":"01","author":[{"last_name":"Lafon Cazal","first_name":"Mireille","full_name":"Lafon Cazal, Mireille"},{"last_name":"Viennois","full_name":"Viennois, Gaëlle","first_name":"Gaëlle"},{"full_name":"Kühn, Rainer","first_name":"Rainer","last_name":"Kühn"},{"first_name":"Barbara","full_name":"Malitschek, Barbara","last_name":"Malitschek"},{"last_name":"Pin","full_name":"Pin, Jean","first_name":"Jean"},{"id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","first_name":"Ryuichi"},{"last_name":"Bockaërt","first_name":"Joël","full_name":"Bockaërt, Joël"}],"publication":"Neuropharmacology","language":[{"iso":"eng"}],"scopus_import":"1","intvolume":"        38","issue":"10","article_processing_charge":"No","date_updated":"2022-09-13T08:23:20Z","title":"mGluR7-like receptor and GABA(B) receptor activation enhance neurotoxic effects of N-methyl-D-aspartate in cultured mouse striatal GABAergic neurones"},{"pmid":1,"status":"public","type":"journal_article","author":[{"full_name":"Bushell, Trevor","first_name":"Trevor","last_name":"Bushell"},{"full_name":"Lee, Chong","first_name":"Chong","last_name":"Lee"},{"id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","first_name":"Ryuichi"},{"last_name":"Miller","first_name":"Richard","full_name":"Miller, Richard"}],"oa_version":"None","day":"01","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publication_identifier":{"issn":["0028-3908"]},"month":"10","language":[{"iso":"eng"}],"publication":"Neuropharmacology","title":"Modulation of synaptic transmission and differential localisation of mGlus in cultured hippocampal autapses","date_updated":"2022-09-13T08:15:55Z","issue":"10","article_processing_charge":"No","scopus_import":"1","intvolume":"        38","extern":"1","page":"1553 - 1567","quality_controlled":"1","volume":38,"publisher":"Elsevier","date_published":"1999-10-01T00:00:00Z","publication_status":"published","external_id":{"pmid":["10530817"]},"abstract":[{"lang":"eng","text":"Metabotropic glutamate receptors (mGlus) are known to modulate synaptic transmission in various pathways of the central nervous system, but the exact mechanisms by which this modulation occurs remain unclear. Here we utilise electrophysiological and immunocytochemical techniques on cultured autaptic hippocampal neurones to investigate the mechanism of action and distribution of mGlus. Agonists at all three groups of mGlus depressed glutamatergic transmission, whereas only agonists at group I mGlus depressed GABAergic transmission. Agonists at all mGlus failed to modulate Ca2+ and K+ channels in glutamatergic autapses whereas an agonist at group III mGlus did depress the frequency of miniature excitatory postsynaptic currents (mEPSCs). Agonists failed to modulate Ca2+ or K+ channels and miniature inhibitory postsynaptic currents (mIPSCs) in GABAergic autapses. Distribution studies using selective antibodies revealed punctate staining for group III mGlus that co-localised with the synaptic marker, synaptophysin. Staining for the remaining mGlus was more diffuse throughout the soma and processes with little co-localisation with synaptophysin. The distribution of the group III receptors is consistent with the direct 'downstream' modulation of mEPSCs, although the exact mechanism of action for the remaining receptors remains unclear."}],"article_type":"original","_id":"2597","doi":"10.1016/S0028-3908(99)00103-3","date_created":"2018-12-11T11:58:35Z","publist_id":"4301","citation":{"apa":"Bushell, T., Lee, C., Shigemoto, R., &#38; Miller, R. (1999). Modulation of synaptic transmission and differential localisation of mGlus in cultured hippocampal autapses. <i>Neuropharmacology</i>. Elsevier. <a href=\"https://doi.org/10.1016/S0028-3908(99)00103-3\">https://doi.org/10.1016/S0028-3908(99)00103-3</a>","short":"T. Bushell, C. Lee, R. Shigemoto, R. Miller, Neuropharmacology 38 (1999) 1553–1567.","ieee":"T. Bushell, C. Lee, R. Shigemoto, and R. Miller, “Modulation of synaptic transmission and differential localisation of mGlus in cultured hippocampal autapses,” <i>Neuropharmacology</i>, vol. 38, no. 10. Elsevier, pp. 1553–1567, 1999.","ama":"Bushell T, Lee C, Shigemoto R, Miller R. Modulation of synaptic transmission and differential localisation of mGlus in cultured hippocampal autapses. <i>Neuropharmacology</i>. 1999;38(10):1553-1567. doi:<a href=\"https://doi.org/10.1016/S0028-3908(99)00103-3\">10.1016/S0028-3908(99)00103-3</a>","ista":"Bushell T, Lee C, Shigemoto R, Miller R. 1999. Modulation of synaptic transmission and differential localisation of mGlus in cultured hippocampal autapses. Neuropharmacology. 38(10), 1553–1567.","chicago":"Bushell, Trevor, Chong Lee, Ryuichi Shigemoto, and Richard Miller. “Modulation of Synaptic Transmission and Differential Localisation of MGlus in Cultured Hippocampal Autapses.” <i>Neuropharmacology</i>. Elsevier, 1999. <a href=\"https://doi.org/10.1016/S0028-3908(99)00103-3\">https://doi.org/10.1016/S0028-3908(99)00103-3</a>.","mla":"Bushell, Trevor, et al. “Modulation of Synaptic Transmission and Differential Localisation of MGlus in Cultured Hippocampal Autapses.” <i>Neuropharmacology</i>, vol. 38, no. 10, Elsevier, 1999, pp. 1553–67, doi:<a href=\"https://doi.org/10.1016/S0028-3908(99)00103-3\">10.1016/S0028-3908(99)00103-3</a>."},"acknowledgement":"This work was supported by a Wellcome International Travel Fellowship to TJB and by the Public Service Grants DA02121, MH40165, NS33502 and NS33826 for CCL and RJM. We are grateful to Dr Graeme I. Bell for use of his microscope for the antibody studies.","year":"1999"}]