---
_id: '14341'
abstract:
- lang: eng
text: Flows through pipes and channels are, in practice, almost always turbulent,
and the multiscale eddying motion is responsible for a major part of the encountered
friction losses and pumping costs1. Conversely, for pulsatile flows, in particular
for aortic blood flow, turbulence levels remain low despite relatively large peak
velocities. For aortic blood flow, high turbulence levels are intolerable as they
would damage the shear-sensitive endothelial cell layer2,3,4,5. Here we show that
turbulence in ordinary pipe flow is diminished if the flow is driven in a pulsatile
mode that incorporates all the key features of the cardiac waveform. At Reynolds
numbers comparable to those of aortic blood flow, turbulence is largely inhibited,
whereas at much higher speeds, the turbulent drag is reduced by more than 25%.
This specific operation mode is more efficient when compared with steady driving,
which is the present situation for virtually all fluid transport processes ranging
from heating circuits to water, gas and oil pipelines.
acknowledged_ssus:
- _id: M-Shop
- _id: ScienComp
acknowledgement: We acknowledge the assistance of the Miba machine shop and the team
of the ISTA-HPC cluster. We thank M. Quadrio for the discussions. The work was supported
by the Simons Foundation (grant no. 662960) and by the Austrian Science Fund (grant
no. I4188-N30), within Deutsche Forschungsgemeinschaft research unit FOR 2688.
article_processing_charge: No
article_type: original
author:
- first_name: Davide
full_name: Scarselli, Davide
id: 40315C30-F248-11E8-B48F-1D18A9856A87
last_name: Scarselli
orcid: 0000-0001-5227-4271
- first_name: Jose M
full_name: Lopez Alonso, Jose M
id: 40770848-F248-11E8-B48F-1D18A9856A87
last_name: Lopez Alonso
orcid: 0000-0002-0384-2022
- first_name: Atul
full_name: Varshney, Atul
id: 2A2006B2-F248-11E8-B48F-1D18A9856A87
last_name: Varshney
orcid: 0000-0002-3072-5999
- first_name: Björn
full_name: Hof, Björn
id: 3A374330-F248-11E8-B48F-1D18A9856A87
last_name: Hof
orcid: 0000-0003-2057-2754
citation:
ama: Scarselli D, Lopez Alonso JM, Varshney A, Hof B. Turbulence suppression by
cardiac-cycle-inspired driving of pipe flow. Nature. 2023;621(7977):71-74.
doi:10.1038/s41586-023-06399-5
apa: Scarselli, D., Lopez Alonso, J. M., Varshney, A., & Hof, B. (2023). Turbulence
suppression by cardiac-cycle-inspired driving of pipe flow. Nature. Springer
Nature. https://doi.org/10.1038/s41586-023-06399-5
chicago: Scarselli, Davide, Jose M Lopez Alonso, Atul Varshney, and Björn Hof. “Turbulence
Suppression by Cardiac-Cycle-Inspired Driving of Pipe Flow.” Nature. Springer
Nature, 2023. https://doi.org/10.1038/s41586-023-06399-5.
ieee: D. Scarselli, J. M. Lopez Alonso, A. Varshney, and B. Hof, “Turbulence suppression
by cardiac-cycle-inspired driving of pipe flow,” Nature, vol. 621, no.
7977. Springer Nature, pp. 71–74, 2023.
ista: Scarselli D, Lopez Alonso JM, Varshney A, Hof B. 2023. Turbulence suppression
by cardiac-cycle-inspired driving of pipe flow. Nature. 621(7977), 71–74.
mla: Scarselli, Davide, et al. “Turbulence Suppression by Cardiac-Cycle-Inspired
Driving of Pipe Flow.” Nature, vol. 621, no. 7977, Springer Nature, 2023,
pp. 71–74, doi:10.1038/s41586-023-06399-5.
short: D. Scarselli, J.M. Lopez Alonso, A. Varshney, B. Hof, Nature 621 (2023) 71–74.
date_created: 2023-09-17T22:01:09Z
date_published: 2023-09-07T00:00:00Z
date_updated: 2023-09-20T12:10:22Z
day: '07'
department:
- _id: BjHo
doi: 10.1038/s41586-023-06399-5
external_id:
pmid:
- '37673988'
intvolume: ' 621'
issue: '7977'
language:
- iso: eng
month: '09'
oa_version: None
page: 71-74
pmid: 1
project:
- _id: 238598C6-32DE-11EA-91FC-C7463DDC885E
grant_number: '662960'
name: 'Revisiting the Turbulence Problem Using Statistical Mechanics: Experimental
Studies on Transitional and Turbulent Flows'
- _id: 238B8092-32DE-11EA-91FC-C7463DDC885E
call_identifier: FWF
grant_number: I04188
name: Instabilities in pulsating pipe flow of Newtonian and complex fluids
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://www.ista.ac.at/en/news/pumping-like-the-heart/
scopus_import: '1'
status: public
title: Turbulence suppression by cardiac-cycle-inspired driving of pipe flow
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 621
year: '2023'
...
---
_id: '14610'
abstract:
- lang: eng
text: AbstractEndomembrane damage represents a
form of stress that is detrimental for eukaryotic cells1,2.
To cope with this threat, cells possess mechanisms that repair the damage and
restore cellular homeostasis3–7. Endomembrane damage also
results in organelle instability and the mechanisms by which cells stabilize damaged
endomembranes to enable membrane repair remains unknown. Here, by combining in
vitro and in cellulo studies with computational modelling we uncover a biological
function for stress granules whereby these biomolecular condensates form rapidly
at endomembrane damage sites and act as a plug that stabilizes the ruptured membrane.
Functionally, we demonstrate that stress granule formation and membrane stabilization
enable efficient repair of damaged endolysosomes, through both ESCRT (endosomal
sorting complex required for transport)-dependent and independent mechanisms.
We also show that blocking stress granule formation in human macrophages creates
a permissive environment for Mycobacterium tuberculosis,
a human pathogen that exploits endomembrane damage to survive within the host.
acknowledgement: "We thank the Human Embryonic Stem Cell Unit, Advanced Light Microscopy
and High-throughput Screening facilities at the Crick for their support in various
aspects of the work. We thank the laboratory of P. Anderson for providing the G3BP-DKO
U2OS cells. The authors thank N. Chen for providing the purified glycinin protein;
Z. Zhao for providing the microfluidic chip wafers; and M. Amaral and F. Frey for
helpful discussions and valuable input regarding analysis methods. This work was
supported by the Francis Crick Institute (to M.G.G.), which receives its core funding
from Cancer Research UK (FC001092), the UK Medical Research Council (FC001092) and
the Wellcome Trust (FC001092). This project has received funding from the European
Research Council (ERC) under the European Union’s Horizon 2020 research and innovation
programme (grant agreement no. 772022 to M.G.G.). C.B. has received funding from
the European Respiratory Society and the European Union’s H2020 research and innovation
programme under the Marie Sklodowska-Curie grant agreement no. 713406. A.M. acknowledges
support from Alexander von Humboldt Foundation and C.V.-C. acknowledges funding
by the Royal Society and the European Research Council under the European Union’s
Horizon 2020 Research and Innovation Programme (grant no. 802960 to A.S.). All simulations
were carried out on the high-performance computing cluster at the Institute of Science
and Technology Austria. For the purpose of Open Access, the author has applied a
CC BY public copyright licence to any Author Accepted Manuscript version arising
from this submission.\r\nOpen Access funding provided by The Francis Crick Institute."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Claudio
full_name: Bussi, Claudio
last_name: Bussi
- first_name: Agustín
full_name: Mangiarotti, Agustín
last_name: Mangiarotti
- first_name: Christian Eduardo
full_name: Vanhille-Campos, Christian Eduardo
id: 3adeca52-9313-11ed-b1ac-c170b2505714
last_name: Vanhille-Campos
- first_name: Beren
full_name: Aylan, Beren
last_name: Aylan
- first_name: Enrica
full_name: Pellegrino, Enrica
last_name: Pellegrino
- first_name: Natalia
full_name: Athanasiadi, Natalia
last_name: Athanasiadi
- first_name: Antony
full_name: Fearns, Antony
last_name: Fearns
- first_name: Angela
full_name: Rodgers, Angela
last_name: Rodgers
- first_name: Titus M.
full_name: Franzmann, Titus M.
last_name: Franzmann
- first_name: Anđela
full_name: Šarić, Anđela
id: bf63d406-f056-11eb-b41d-f263a6566d8b
last_name: Šarić
orcid: 0000-0002-7854-2139
- first_name: Rumiana
full_name: Dimova, Rumiana
last_name: Dimova
- first_name: Maximiliano G.
full_name: Gutierrez, Maximiliano G.
last_name: Gutierrez
citation:
ama: Bussi C, Mangiarotti A, Vanhille-Campos CE, et al. Stress granules plug and
stabilize damaged endolysosomal membranes. Nature. 2023. doi:10.1038/s41586-023-06726-w
apa: Bussi, C., Mangiarotti, A., Vanhille-Campos, C. E., Aylan, B., Pellegrino,
E., Athanasiadi, N., … Gutierrez, M. G. (2023). Stress granules plug and stabilize
damaged endolysosomal membranes. Nature. Springer Nature. https://doi.org/10.1038/s41586-023-06726-w
chicago: Bussi, Claudio, Agustín Mangiarotti, Christian Eduardo Vanhille-Campos,
Beren Aylan, Enrica Pellegrino, Natalia Athanasiadi, Antony Fearns, et al. “Stress
Granules Plug and Stabilize Damaged Endolysosomal Membranes.” Nature. Springer
Nature, 2023. https://doi.org/10.1038/s41586-023-06726-w.
ieee: C. Bussi et al., “Stress granules plug and stabilize damaged endolysosomal
membranes,” Nature. Springer Nature, 2023.
ista: Bussi C, Mangiarotti A, Vanhille-Campos CE, Aylan B, Pellegrino E, Athanasiadi
N, Fearns A, Rodgers A, Franzmann TM, Šarić A, Dimova R, Gutierrez MG. 2023. Stress
granules plug and stabilize damaged endolysosomal membranes. Nature.
mla: Bussi, Claudio, et al. “Stress Granules Plug and Stabilize Damaged Endolysosomal
Membranes.” Nature, Springer Nature, 2023, doi:10.1038/s41586-023-06726-w.
short: C. Bussi, A. Mangiarotti, C.E. Vanhille-Campos, B. Aylan, E. Pellegrino,
N. Athanasiadi, A. Fearns, A. Rodgers, T.M. Franzmann, A. Šarić, R. Dimova, M.G.
Gutierrez, Nature (2023).
date_created: 2023-11-27T07:56:37Z
date_published: 2023-11-15T00:00:00Z
date_updated: 2023-11-27T09:05:08Z
day: '15'
department:
- _id: AnSa
doi: 10.1038/s41586-023-06726-w
external_id:
pmid:
- '37968398'
keyword:
- Multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41586-023-06726-w
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: epub_ahead
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1038/s41586-023-06882-z
record:
- id: '14472'
relation: research_data
status: public
status: public
title: Stress granules plug and stabilize damaged endolysosomal membranes
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '13096'
abstract:
- lang: eng
text: Eukaryotic cells can undergo different forms of programmed cell death, many
of which culminate in plasma membrane rupture as the defining terminal event1,2,3,4,5,6,7.
Plasma membrane rupture was long thought to be driven by osmotic pressure, but
it has recently been shown to be in many cases an active process, mediated by
the protein ninjurin-18 (NINJ1). Here we resolve the structure of NINJ1 and the
mechanism by which it ruptures membranes. Super-resolution microscopy reveals
that NINJ1 clusters into structurally diverse assemblies in the membranes of dying
cells, in particular large, filamentous assemblies with branched morphology. A
cryo-electron microscopy structure of NINJ1 filaments shows a tightly packed fence-like
array of transmembrane α-helices. Filament directionality and stability is defined
by two amphipathic α-helices that interlink adjacent filament subunits. The NINJ1
filament features a hydrophilic side and a hydrophobic side, and molecular dynamics
simulations show that it can stably cap membrane edges. The function of the resulting
supramolecular arrangement was validated by site-directed mutagenesis. Our data
thus suggest that, during lytic cell death, the extracellular α-helices of NINJ1
insert into the plasma membrane to polymerize NINJ1 monomers into amphipathic
filaments that rupture the plasma membrane. The membrane protein NINJ1 is therefore
an interactive component of the eukaryotic cell membrane that functions as an
in-built breaking point in response to activation of cell death.
acknowledged_ssus:
- _id: NMR
- _id: LifeSc
acknowledgement: "This work was supported by the Deutsche Forschungsgemeinschaft under
Germany’s Excellence Strategy EXC 2075–390740016 and the Stuttgart Center for Simulation
Science (SC SimTech) to K.P., by ERC-CoG 770988 (InflamCellDeath) and SNF Project
funding (310030B_198005, 310030B_192523) to P.B., by the Swiss Nanoscience Institute
and the Swiss National Science Foundation via the NCCR AntiResist (180541) to S.H.
and the NCCR Molecular Systems Engineering (51NF40-205608) to D.J.M., by the Helmholtz
Young Investigator Program of the Helmholtz Association to C.S., by the SNF Professorship
funding (PP00P3_198903) to C.P., EMBO postdoctoral fellowship ALTF 27-2022 to E.H.
and by the Scientific Service Units of IST Austria through resources provided by
the NMR and Life Science Facilities to P.S. Molecular dynamics simulations were
performed on the HoreKa supercomputer funded by the Ministry of Science, Research
and the Arts Baden-Württemberg and by the Federal Ministry of Education and Research.
The authors thank the BioEM Lab of the Biozentrum, University of Basel for support;
V. Mack, K. Shkarina and J. Fricke for technical support; D. Ricklin and S. Vogt
for peptide synthesis; P. Pelczar for support with animals; S.-J. Marrink and P.
Telles de Souza for supply with Martini3 parameters and scripts; and P. Radler und
M. Loose for help with QCM. Fig. 4g and Extended Data Fig. 1a were in part created
with BioRender.com.\r\nOpen access funding provided by University of Basel."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Morris
full_name: Degen, Morris
last_name: Degen
- first_name: José Carlos
full_name: Santos, José Carlos
last_name: Santos
- first_name: Kristyna
full_name: Pluhackova, Kristyna
last_name: Pluhackova
- first_name: Gonzalo
full_name: Cebrero, Gonzalo
last_name: Cebrero
- first_name: Saray
full_name: Ramos, Saray
last_name: Ramos
- first_name: Gytis
full_name: Jankevicius, Gytis
last_name: Jankevicius
- first_name: Ella
full_name: Hartenian, Ella
last_name: Hartenian
- first_name: Undina
full_name: Guillerm, Undina
id: bb74f472-ae54-11eb-9835-bc9c22fb1183
last_name: Guillerm
- first_name: Stefania A.
full_name: Mari, Stefania A.
last_name: Mari
- first_name: Bastian
full_name: Kohl, Bastian
last_name: Kohl
- first_name: Daniel J.
full_name: Müller, Daniel J.
last_name: Müller
- first_name: Paul
full_name: Schanda, Paul
id: 7B541462-FAF6-11E9-A490-E8DFE5697425
last_name: Schanda
orcid: 0000-0002-9350-7606
- first_name: Timm
full_name: Maier, Timm
last_name: Maier
- first_name: Camilo
full_name: Perez, Camilo
last_name: Perez
- first_name: Christian
full_name: Sieben, Christian
last_name: Sieben
- first_name: Petr
full_name: Broz, Petr
last_name: Broz
- first_name: Sebastian
full_name: Hiller, Sebastian
last_name: Hiller
citation:
ama: Degen M, Santos JC, Pluhackova K, et al. Structural basis of NINJ1-mediated
plasma membrane rupture in cell death. Nature. 2023;618:1065-1071. doi:10.1038/s41586-023-05991-z
apa: Degen, M., Santos, J. C., Pluhackova, K., Cebrero, G., Ramos, S., Jankevicius,
G., … Hiller, S. (2023). Structural basis of NINJ1-mediated plasma membrane rupture
in cell death. Nature. Springer Nature. https://doi.org/10.1038/s41586-023-05991-z
chicago: Degen, Morris, José Carlos Santos, Kristyna Pluhackova, Gonzalo Cebrero,
Saray Ramos, Gytis Jankevicius, Ella Hartenian, et al. “Structural Basis of NINJ1-Mediated
Plasma Membrane Rupture in Cell Death.” Nature. Springer Nature, 2023.
https://doi.org/10.1038/s41586-023-05991-z.
ieee: M. Degen et al., “Structural basis of NINJ1-mediated plasma membrane
rupture in cell death,” Nature, vol. 618. Springer Nature, pp. 1065–1071,
2023.
ista: Degen M, Santos JC, Pluhackova K, Cebrero G, Ramos S, Jankevicius G, Hartenian
E, Guillerm U, Mari SA, Kohl B, Müller DJ, Schanda P, Maier T, Perez C, Sieben
C, Broz P, Hiller S. 2023. Structural basis of NINJ1-mediated plasma membrane
rupture in cell death. Nature. 618, 1065–1071.
mla: Degen, Morris, et al. “Structural Basis of NINJ1-Mediated Plasma Membrane Rupture
in Cell Death.” Nature, vol. 618, Springer Nature, 2023, pp. 1065–71, doi:10.1038/s41586-023-05991-z.
short: M. Degen, J.C. Santos, K. Pluhackova, G. Cebrero, S. Ramos, G. Jankevicius,
E. Hartenian, U. Guillerm, S.A. Mari, B. Kohl, D.J. Müller, P. Schanda, T. Maier,
C. Perez, C. Sieben, P. Broz, S. Hiller, Nature 618 (2023) 1065–1071.
date_created: 2023-05-28T22:01:04Z
date_published: 2023-06-29T00:00:00Z
date_updated: 2023-11-14T11:49:21Z
day: '29'
ddc:
- '570'
department:
- _id: PaSc
doi: 10.1038/s41586-023-05991-z
external_id:
isi:
- '000991386800011'
file:
- access_level: open_access
checksum: 0fab69252453bff1de7f0e2eceb76d34
content_type: application/pdf
creator: dernst
date_created: 2023-11-14T11:48:18Z
date_updated: 2023-11-14T11:48:18Z
file_id: '14533'
file_name: 2023_Nature_Degen.pdf
file_size: 12292188
relation: main_file
success: 1
file_date_updated: 2023-11-14T11:48:18Z
has_accepted_license: '1'
intvolume: ' 618'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1065-1071
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Structural basis of NINJ1-mediated plasma membrane rupture in cell death
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 618
year: '2023'
...
---
_id: '13119'
abstract:
- lang: eng
text: A density wave (DW) is a fundamental type of long-range order in quantum matter
tied to self-organization into a crystalline structure. The interplay of DW order
with superfluidity can lead to complex scenarios that pose a great challenge to
theoretical analysis. In the past decades, tunable quantum Fermi gases have served
as model systems for exploring the physics of strongly interacting fermions, including
most notably magnetic ordering1, pairing and superfluidity2, and the crossover
from a Bardeen–Cooper–Schrieffer superfluid to a Bose–Einstein condensate3. Here,
we realize a Fermi gas featuring both strong, tunable contact interactions and
photon-mediated, spatially structured long-range interactions in a transversely
driven high-finesse optical cavity. Above a critical long-range interaction strength,
DW order is stabilized in the system, which we identify via its superradiant light-scattering
properties. We quantitatively measure the variation of the onset of DW order as
the contact interaction is varied across the Bardeen–Cooper–Schrieffer superfluid
and Bose–Einstein condensate crossover, in qualitative agreement with a mean-field
theory. The atomic DW susceptibility varies over an order of magnitude upon tuning
the strength and the sign of the long-range interactions below the self-ordering
threshold, demonstrating independent and simultaneous control over the contact
and long-range interactions. Therefore, our experimental setup provides a fully
tunable and microscopically controllable platform for the experimental study of
the interplay of superfluidity and DW order.
acknowledgement: Open access funding provided by EPFL Lausanne.We acknowledge discussions
with T. Donner and T. Esslinger. We thank G. del Pace and T. Bühler for their assistance
in the final stages of the experiment. We acknowledge funding from the European
Research Council under the European Union Horizon 2020 Research and Innovation Programme
(Grant no. 714309) and the Swiss National Science Foundation (Grant no. 184654).
F.M. acknowledges financial support from the Austrian Science Fund (Stand-Alone
Project P 35891-N).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Victor
full_name: Helson, Victor
last_name: Helson
- first_name: Timo
full_name: Zwettler, Timo
last_name: Zwettler
- first_name: Farokh
full_name: Mivehvar, Farokh
last_name: Mivehvar
- first_name: Elvia
full_name: Colella, Elvia
last_name: Colella
- first_name: Kevin Etienne Robert
full_name: Roux, Kevin Etienne Robert
id: 53f93ea2-803f-11ed-ab7e-b283135794ef
last_name: Roux
- first_name: Hideki
full_name: Konishi, Hideki
last_name: Konishi
- first_name: Helmut
full_name: Ritsch, Helmut
last_name: Ritsch
- first_name: Jean Philippe
full_name: Brantut, Jean Philippe
last_name: Brantut
citation:
ama: Helson V, Zwettler T, Mivehvar F, et al. Density-wave ordering in a unitary
Fermi gas with photon-mediated interactions. Nature. 2023;618:716-720.
doi:10.1038/s41586-023-06018-3
apa: Helson, V., Zwettler, T., Mivehvar, F., Colella, E., Roux, K. E. R., Konishi,
H., … Brantut, J. P. (2023). Density-wave ordering in a unitary Fermi gas with
photon-mediated interactions. Nature. Springer Nature. https://doi.org/10.1038/s41586-023-06018-3
chicago: Helson, Victor, Timo Zwettler, Farokh Mivehvar, Elvia Colella, Kevin Etienne
Robert Roux, Hideki Konishi, Helmut Ritsch, and Jean Philippe Brantut. “Density-Wave
Ordering in a Unitary Fermi Gas with Photon-Mediated Interactions.” Nature.
Springer Nature, 2023. https://doi.org/10.1038/s41586-023-06018-3.
ieee: V. Helson et al., “Density-wave ordering in a unitary Fermi gas with
photon-mediated interactions,” Nature, vol. 618. Springer Nature, pp. 716–720,
2023.
ista: Helson V, Zwettler T, Mivehvar F, Colella E, Roux KER, Konishi H, Ritsch H,
Brantut JP. 2023. Density-wave ordering in a unitary Fermi gas with photon-mediated
interactions. Nature. 618, 716–720.
mla: Helson, Victor, et al. “Density-Wave Ordering in a Unitary Fermi Gas with Photon-Mediated
Interactions.” Nature, vol. 618, Springer Nature, 2023, pp. 716–20, doi:10.1038/s41586-023-06018-3.
short: V. Helson, T. Zwettler, F. Mivehvar, E. Colella, K.E.R. Roux, H. Konishi,
H. Ritsch, J.P. Brantut, Nature 618 (2023) 716–720.
date_created: 2023-06-04T22:01:03Z
date_published: 2023-06-22T00:00:00Z
date_updated: 2023-11-14T13:02:50Z
day: '22'
ddc:
- '530'
department:
- _id: GeKa
doi: 10.1038/s41586-023-06018-3
external_id:
isi:
- '001001139300008'
file:
- access_level: open_access
checksum: 4887a296e3b6f54e8c0b946cbfd24f49
content_type: application/pdf
creator: dernst
date_created: 2023-11-14T13:00:19Z
date_updated: 2023-11-14T13:00:19Z
file_id: '14534'
file_name: 2023_Nature_Helson.pdf
file_size: 8156497
relation: main_file
success: 1
file_date_updated: 2023-11-14T13:00:19Z
has_accepted_license: '1'
intvolume: ' 618'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 716-720
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Density-wave ordering in a unitary Fermi gas with photon-mediated interactions
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 618
year: '2023'
...
---
_id: '11341'
abstract:
- lang: eng
text: Intragenic regions that are removed during maturation of the RNA transcript—introns—are
universally present in the nuclear genomes of eukaryotes1. The budding yeast,
an otherwise intron-poor species, preserves two sets of ribosomal protein genes
that differ primarily in their introns2,3. Although studies have shed light on
the role of ribosomal protein introns under stress and starvation4,5,6, understanding
the contribution of introns to ribosome regulation remains challenging. Here,
by combining isogrowth profiling7 with single-cell protein measurements8, we show
that introns can mediate inducible phenotypic heterogeneity that confers a clear
fitness advantage. Osmotic stress leads to bimodal expression of the small ribosomal
subunit protein Rps22B, which is mediated by an intron in the 5′ untranslated
region of its transcript. The two resulting yeast subpopulations differ in their
ability to cope with starvation. Low levels of Rps22B protein result in prolonged
survival under sustained starvation, whereas high levels of Rps22B enable cells
to grow faster after transient starvation. Furthermore, yeasts growing at high
concentrations of sugar, similar to those in ripe grapes, exhibit bimodal expression
of Rps22B when approaching the stationary phase. Differential intron-mediated
regulation of ribosomal protein genes thus provides a way to diversify the population
when starvation threatens in natural environments. Our findings reveal a role
for introns in inducing phenotypic heterogeneity in changing environments, and
suggest that duplicated ribosomal protein genes in yeast contribute to resolving
the evolutionary conflict between precise expression control and environmental
responsiveness9.
acknowledged_ssus:
- _id: LifeSc
- _id: M-Shop
- _id: Bio
acknowledgement: We thank the IST Austria Life Science Facility, the Miba Machine
Shop and M. Lukačišinová for support with the liquid handling robot; the Bioimaging
Facility at IST Austria, J. Power and B. Meier at the University of Cologne, and
C. Göttlinger at the FACS Analysis Facility at the Institute for Genetics, University
of Cologne, for support with flow cytometry experiments; L. Horst for the development
of the automated experimental methods in Cologne; J. Parenteau, S. Abou Elela, G.
Stormo, M. Springer and M. Schuldiner for providing us with yeast strains; B. Fernando,
T. Fink, G. Ansmann and G. Chevreau for technical support; H. Köver, G. Tkačik,
N. Barton, A. Angermayr and B. Kavčič for support during laboratory relocation;
D. Siekhaus, M. Springer and all the members of the Bollenbach group for support
and discussions; and K. Mitosch, M. Lukačišinová, G. Liti and A. de Luna for critical
reading of our manuscript. This work was supported in part by an Austrian Science
Fund (FWF) standalone grant P 27201-B22 (to T.B.), HFSP program Grant RGP0042/2013
(to T.B.), EU Marie Curie Career Integration Grant No. 303507, and German Research
Foundation (DFG) Collaborative Research Centre (SFB) 1310 (to T.B.). A.E.-C. was
supported by a Georg Forster fellowship from the Alexander von Humboldt Foundation.
article_processing_charge: No
article_type: original
author:
- first_name: Martin
full_name: Lukacisin, Martin
id: 298FFE8C-F248-11E8-B48F-1D18A9856A87
last_name: Lukacisin
orcid: 0000-0001-6549-4177
- first_name: Adriana
full_name: Espinosa-Cantú, Adriana
last_name: Espinosa-Cantú
- first_name: Mark Tobias
full_name: Bollenbach, Mark Tobias
id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
last_name: Bollenbach
orcid: 0000-0003-4398-476X
citation:
ama: Lukacisin M, Espinosa-Cantú A, Bollenbach MT. Intron-mediated induction of
phenotypic heterogeneity. Nature. 2022;605:113-118. doi:10.1038/s41586-022-04633-0
apa: Lukacisin, M., Espinosa-Cantú, A., & Bollenbach, M. T. (2022). Intron-mediated
induction of phenotypic heterogeneity. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-04633-0
chicago: Lukacisin, Martin, Adriana Espinosa-Cantú, and Mark Tobias Bollenbach.
“Intron-Mediated Induction of Phenotypic Heterogeneity.” Nature. Springer
Nature, 2022. https://doi.org/10.1038/s41586-022-04633-0.
ieee: M. Lukacisin, A. Espinosa-Cantú, and M. T. Bollenbach, “Intron-mediated induction
of phenotypic heterogeneity,” Nature, vol. 605. Springer Nature, pp. 113–118,
2022.
ista: Lukacisin M, Espinosa-Cantú A, Bollenbach MT. 2022. Intron-mediated induction
of phenotypic heterogeneity. Nature. 605, 113–118.
mla: Lukacisin, Martin, et al. “Intron-Mediated Induction of Phenotypic Heterogeneity.”
Nature, vol. 605, Springer Nature, 2022, pp. 113–18, doi:10.1038/s41586-022-04633-0.
short: M. Lukacisin, A. Espinosa-Cantú, M.T. Bollenbach, Nature 605 (2022) 113–118.
date_created: 2022-05-01T22:01:42Z
date_published: 2022-05-05T00:00:00Z
date_updated: 2023-08-03T06:44:50Z
day: '05'
ddc:
- '570'
doi: 10.1038/s41586-022-04633-0
ec_funded: 1
external_id:
isi:
- '000784934100003'
pmid:
- '35444278'
file:
- access_level: open_access
checksum: d68cd1596bb9fd819b750fe47c8a138a
content_type: application/pdf
creator: dernst
date_created: 2022-08-05T06:08:24Z
date_updated: 2022-08-05T06:08:24Z
file_id: '11727'
file_name: 2022_Nature_Lukacisin.pdf
file_size: 25360311
relation: main_file
success: 1
file_date_updated: 2022-08-05T06:08:24Z
has_accepted_license: '1'
intvolume: ' 605'
isi: 1
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 113-118
pmid: 1
project:
- _id: 25E83C2C-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '303507'
name: Optimality principles in responses to antibiotics
- _id: 25E9AF9E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P27201-B22
name: Revealing the mechanisms underlying drug interactions
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Intron-mediated induction of phenotypic heterogeneity
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 605
year: '2022'
...
---
_id: '14437'
abstract:
- lang: eng
text: Future LEDs could be based on lead halide perovskites. A breakthrough in preparing
device-compatible solids composed of nanoscale perovskite crystals overcomes a
long-standing hurdle in making blue perovskite LEDs.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Hendrik
full_name: Utzat, Hendrik
last_name: Utzat
- first_name: Maria
full_name: Ibáñez, Maria
id: 43C61214-F248-11E8-B48F-1D18A9856A87
last_name: Ibáñez
orcid: 0000-0001-5013-2843
citation:
ama: Utzat H, Ibáñez M. Molecular engineering enables bright blue LEDs. Nature.
2022;612(7941):638-639. doi:10.1038/d41586-022-04447-0
apa: Utzat, H., & Ibáñez, M. (2022). Molecular engineering enables bright blue
LEDs. Nature. Springer Nature. https://doi.org/10.1038/d41586-022-04447-0
chicago: Utzat, Hendrik, and Maria Ibáñez. “Molecular Engineering Enables Bright
Blue LEDs.” Nature. Springer Nature, 2022. https://doi.org/10.1038/d41586-022-04447-0.
ieee: H. Utzat and M. Ibáñez, “Molecular engineering enables bright blue LEDs,”
Nature, vol. 612, no. 7941. Springer Nature, pp. 638–639, 2022.
ista: Utzat H, Ibáñez M. 2022. Molecular engineering enables bright blue LEDs. Nature.
612(7941), 638–639.
mla: Utzat, Hendrik, and Maria Ibáñez. “Molecular Engineering Enables Bright Blue
LEDs.” Nature, vol. 612, no. 7941, Springer Nature, 2022, pp. 638–39, doi:10.1038/d41586-022-04447-0.
short: H. Utzat, M. Ibáñez, Nature 612 (2022) 638–639.
date_created: 2023-10-17T11:14:43Z
date_published: 2022-12-21T00:00:00Z
date_updated: 2023-10-18T06:26:30Z
day: '21'
department:
- _id: MaIb
doi: 10.1038/d41586-022-04447-0
external_id:
pmid:
- '36543947'
intvolume: ' 612'
issue: '7941'
keyword:
- Multidisciplinary
language:
- iso: eng
month: '12'
oa_version: None
page: 638-639
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
status: public
title: Molecular engineering enables bright blue LEDs
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 612
year: '2022'
...
---
_id: '12054'
abstract:
- lang: eng
text: 'Polar auxin transport is unique to plants and coordinates their growth and
development1,2. The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical
localizations at the plasma membrane and drive polar auxin transport3,4; however,
their structures and transport mechanisms remain largely unknown. Here, we report
three inward-facing conformation structures of Arabidopsis thaliana PIN1: the
apo state, bound to the natural auxin indole-3-acetic acid (IAA), and in complex
with the polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). The
transmembrane domain of PIN1 shares a conserved NhaA fold5. In the substrate-bound
structure, IAA is coordinated by both hydrophobic stacking and hydrogen bonding.
NPA competes with IAA for the same site at the intracellular pocket, but with
a much higher affinity. These findings inform our understanding of the substrate
recognition and transport mechanisms of PINs and set up a framework for future
research on directional auxin transport, one of the most crucial processes underlying
plant development.'
acknowledgement: We thank the Cryo-EM Center of the University of Science and Technology
of China (USTC) and the Center for Biological Imaging (CBI), Institute of Biophysics,
Chinese Academy of Science, for the EM facility support; we thank B. Zhu, X. Huang
and all the other staff members for their technical support on cryo-EM data collection.
We thank J. Ren for his technical support with the transport assays and M. Seeger
for providing the sybody libraries. This work was supported by the Strategic Priority
Research Program of Chinese Academy of Sciences (XDB 37020204 to D.L. and XDB37020103
to Linfeng Sun), National Natural Science Foundation of China (82151215 and 31870726
to D.L., 31900885 to X.L., and 31870732 to Linfeng Sun), Natural Science Foundation
of Anhui Province (2008085MC90 to X.L. and 2008085J15 to Linfeng Sun), the Fundamental
Research Funds for the Central Universities (WK9100000031 to Linfeng Sun), and the
USTC Research Funds of the Double First-Class Initiative (YD9100002004 to Linfeng
Sun). Linfeng Sun is supported by an Outstanding Young Scholar Award from the Qiu
Shi Science and Technologies Foundation, and a Young Scholar Award from the Cyrus
Tang Foundation.
article_processing_charge: No
article_type: original
author:
- first_name: Z
full_name: Yang, Z
last_name: Yang
- first_name: J
full_name: Xia, J
last_name: Xia
- first_name: J
full_name: Hong, J
last_name: Hong
- first_name: C
full_name: Zhang, C
last_name: Zhang
- first_name: H
full_name: Wei, H
last_name: Wei
- first_name: W
full_name: Ying, W
last_name: Ying
- first_name: C
full_name: Sun, C
last_name: Sun
- first_name: L
full_name: Sun, L
last_name: Sun
- first_name: Y
full_name: Mao, Y
last_name: Mao
- first_name: Y
full_name: Gao, Y
last_name: Gao
- first_name: S
full_name: Tan, S
last_name: Tan
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: D
full_name: Li, D
last_name: Li
- first_name: X
full_name: Liu, X
last_name: Liu
- first_name: L
full_name: Sun, L
last_name: Sun
citation:
ama: Yang Z, Xia J, Hong J, et al. Structural insights into auxin recognition and
efflux by Arabidopsis PIN1. Nature. 2022;609(7927):611-615. doi:10.1038/s41586-022-05143-9
apa: Yang, Z., Xia, J., Hong, J., Zhang, C., Wei, H., Ying, W., … Sun, L. (2022).
Structural insights into auxin recognition and efflux by Arabidopsis PIN1. Nature.
Springer Nature. https://doi.org/10.1038/s41586-022-05143-9
chicago: Yang, Z, J Xia, J Hong, C Zhang, H Wei, W Ying, C Sun, et al. “Structural
Insights into Auxin Recognition and Efflux by Arabidopsis PIN1.” Nature.
Springer Nature, 2022. https://doi.org/10.1038/s41586-022-05143-9.
ieee: Z. Yang et al., “Structural insights into auxin recognition and efflux
by Arabidopsis PIN1,” Nature, vol. 609, no. 7927. Springer Nature, pp.
611–615, 2022.
ista: Yang Z, Xia J, Hong J, Zhang C, Wei H, Ying W, Sun C, Sun L, Mao Y, Gao Y,
Tan S, Friml J, Li D, Liu X, Sun L. 2022. Structural insights into auxin recognition
and efflux by Arabidopsis PIN1. Nature. 609(7927), 611–615.
mla: Yang, Z., et al. “Structural Insights into Auxin Recognition and Efflux by
Arabidopsis PIN1.” Nature, vol. 609, no. 7927, Springer Nature, 2022, pp.
611–15, doi:10.1038/s41586-022-05143-9.
short: Z. Yang, J. Xia, J. Hong, C. Zhang, H. Wei, W. Ying, C. Sun, L. Sun, Y. Mao,
Y. Gao, S. Tan, J. Friml, D. Li, X. Liu, L. Sun, Nature 609 (2022) 611–615.
date_created: 2022-09-07T14:19:52Z
date_published: 2022-08-02T00:00:00Z
date_updated: 2023-08-03T13:41:44Z
day: '02'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41586-022-05143-9
external_id:
isi:
- '000848082900002'
pmid:
- '35917925'
file:
- access_level: open_access
checksum: 3136a585f8e1c7e73b5e1418b3d01898
content_type: application/pdf
creator: dernst
date_created: 2022-09-08T08:02:54Z
date_updated: 2022-09-08T08:02:54Z
file_id: '12064'
file_name: 2022_Nature_Yang.pdf
file_size: 32344580
relation: main_file
success: 1
file_date_updated: 2022-09-08T08:02:54Z
has_accepted_license: '1'
intvolume: ' 609'
isi: 1
issue: '7927'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 611-615
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Structural insights into auxin recognition and efflux by Arabidopsis PIN1
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 609
year: '2022'
...
---
_id: '12118'
abstract:
- lang: eng
text: Hybrid semiconductor–superconductor devices hold great promise for realizing
topological quantum computing with Majorana zero modes1,2,3,4,5. However, multiple
claims of Majorana detection, based on either tunnelling6,7,8,9,10 or Coulomb
blockade (CB) spectroscopy11,12, remain disputed. Here we devise an experimental
protocol that allows us to perform both types of measurement on the same hybrid
island by adjusting its charging energy via tunable junctions to the normal leads.
This method reduces ambiguities of Majorana detections by checking the consistency
between CB spectroscopy and zero-bias peaks in non-blockaded transport. Specifically,
we observe junction-dependent, even–odd modulated, single-electron CB peaks in
InAs/Al hybrid nanowires without concomitant low-bias peaks in tunnelling spectroscopy.
We provide a theoretical interpretation of the experimental observations in terms
of low-energy, longitudinally confined island states rather than overlapping Majorana
modes. Our results highlight the importance of combined measurements on the same
device for the identification of topological Majorana zero modes.
acknowledged_ssus:
- _id: M-Shop
- _id: NanoFab
acknowledgement: We thank P. Krogstrup for providing us with the NW materials. We
thank A. Higginbotham, E. J. H. Lee, C. Marcus and S. Vaitiekėnas for helpful discussions
and G. Steffensen for his input on the diffusive Little-Parks theory. This research
was supported by the Scientific Service Units of ISTA through resources provided
by the MIBA Machine Shop and the nanofabrication facility; the NOMIS Foundation;
the CSIC Interdisciplinary Thematic Platform (PTI+) on Quantum Technologies (PTI-QTEP+).
A.H. acknowledges support from H2020-MSCA-IF-2018/844511. ICN2 also acknowledges
funding from Generalitat de Catalunya 2017 SGR 327. ICN2 is supported by the Severo
Ochoa Program from Spanish MINECO (Grant no. SEV-2017-0706) and is funded by the
CERCA Programme/Generalitat de Catalunya. Part of the present work has been performed
in the framework of Universitat Autònoma de Barcelona Materials Science PhD programme.
Authors acknowledge the use of instrumentation as well as the technical advice provided
by the National Facility ELECMI ICTS, node ‘Laboratorio de Microscopías Avanzadas’
at University of Zaragoza. This project has received funding from the European Union’s
Horizon 2020 research and innovation programme under grant agreement no. 823717-ESTEEM3.
This study was supported by MCIN with funding from European Union NextGenerationEU
(PRTR-C17.I1) and Generalitat de Catalunya. This research is part of the CSIC programme
for the Spanish Recovery, Transformation and Resilience Plan funded by the Recovery
and Resilience Facility of the European Union, established by the Regulation (EU)
2020/2094. We thank support from Grant PGC2018-097018-BI00, project FlagERA TOPOGRAPH
(PCI2018-093026) and project NANOGEN (PID2020-116093RB-C43), funded by MCIN/AEI/10.13039/501100011033/
and by ‘ERDF A way of making Europe’, by the European Union. M. Botifoll acknowledges
support from SUR Generalitat de Catalunya and the EU Social Fund (project ref. 2020
FI 00103).
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Marco
full_name: Valentini, Marco
id: C0BB2FAC-D767-11E9-B658-BC13E6697425
last_name: Valentini
- first_name: Maksim
full_name: Borovkov, Maksim
id: 2ac7a0a2-3562-11eb-9256-fbd18ea55087
last_name: Borovkov
- first_name: Elsa
full_name: Prada, Elsa
last_name: Prada
- first_name: Sara
full_name: Martí-Sánchez, Sara
last_name: Martí-Sánchez
- first_name: Marc
full_name: Botifoll, Marc
last_name: Botifoll
- first_name: Andrea C
full_name: Hofmann, Andrea C
id: 340F461A-F248-11E8-B48F-1D18A9856A87
last_name: Hofmann
- first_name: Jordi
full_name: Arbiol, Jordi
last_name: Arbiol
- first_name: Ramón
full_name: Aguado, Ramón
last_name: Aguado
- first_name: Pablo
full_name: San-Jose, Pablo
last_name: San-Jose
- first_name: Georgios
full_name: Katsaros, Georgios
id: 38DB5788-F248-11E8-B48F-1D18A9856A87
last_name: Katsaros
orcid: 0000-0001-8342-202X
citation:
ama: Valentini M, Borovkov M, Prada E, et al. Majorana-like Coulomb spectroscopy
in the absence of zero-bias peaks. Nature. 2022;612(7940):442-447. doi:10.1038/s41586-022-05382-w
apa: Valentini, M., Borovkov, M., Prada, E., Martí-Sánchez, S., Botifoll, M., Hofmann,
A. C., … Katsaros, G. (2022). Majorana-like Coulomb spectroscopy in the absence
of zero-bias peaks. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05382-w
chicago: Valentini, Marco, Maksim Borovkov, Elsa Prada, Sara Martí-Sánchez, Marc
Botifoll, Andrea C Hofmann, Jordi Arbiol, Ramón Aguado, Pablo San-Jose, and Georgios
Katsaros. “Majorana-like Coulomb Spectroscopy in the Absence of Zero-Bias Peaks.”
Nature. Springer Nature, 2022. https://doi.org/10.1038/s41586-022-05382-w.
ieee: M. Valentini et al., “Majorana-like Coulomb spectroscopy in the absence
of zero-bias peaks,” Nature, vol. 612, no. 7940. Springer Nature, pp. 442–447,
2022.
ista: Valentini M, Borovkov M, Prada E, Martí-Sánchez S, Botifoll M, Hofmann AC,
Arbiol J, Aguado R, San-Jose P, Katsaros G. 2022. Majorana-like Coulomb spectroscopy
in the absence of zero-bias peaks. Nature. 612(7940), 442–447.
mla: Valentini, Marco, et al. “Majorana-like Coulomb Spectroscopy in the Absence
of Zero-Bias Peaks.” Nature, vol. 612, no. 7940, Springer Nature, 2022,
pp. 442–47, doi:10.1038/s41586-022-05382-w.
short: M. Valentini, M. Borovkov, E. Prada, S. Martí-Sánchez, M. Botifoll, A.C.
Hofmann, J. Arbiol, R. Aguado, P. San-Jose, G. Katsaros, Nature 612 (2022) 442–447.
date_created: 2023-01-12T11:56:45Z
date_published: 2022-12-15T00:00:00Z
date_updated: 2024-02-21T12:35:33Z
day: '15'
department:
- _id: GeKa
doi: 10.1038/s41586-022-05382-w
ec_funded: 1
external_id:
arxiv:
- '2203.07829'
isi:
- '000899725400001'
intvolume: ' 612'
isi: 1
issue: '7940'
keyword:
- Multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: ' https://doi.org/10.48550/arXiv.2203.07829'
month: '12'
oa: 1
oa_version: Preprint
page: 442-447
project:
- _id: 26A151DA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '844511'
name: Majorana bound states in Ge/SiGe heterostructures
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA Website
relation: press_release
url: https://ista.ac.at/en/news/imposter-particles-revealed-and-explained/
record:
- id: '13286'
relation: dissertation_contains
status: public
- id: '12522'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Majorana-like Coulomb spectroscopy in the absence of zero-bias peaks
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 612
year: '2022'
...
---
_id: '12138'
abstract:
- lang: eng
text: 'Complex I is the first enzyme in the respiratory chain, which is responsible
for energy production in mitochondria and bacteria1. Complex I couples the transfer
of two electrons from NADH to quinone and the translocation of four protons across
the membrane2, but the coupling mechanism remains contentious. Here we present
cryo-electron microscopy structures of Escherichia coli complex I (EcCI) in different
redox states, including catalytic turnover. EcCI exists mostly in the open state,
in which the quinone cavity is exposed to the cytosol, allowing access for water
molecules, which enable quinone movements. Unlike the mammalian paralogues3, EcCI
can convert to the closed state only during turnover, showing that closed and
open states are genuine turnover intermediates. The open-to-closed transition
results in the tightly engulfed quinone cavity being connected to the central
axis of the membrane arm, a source of substrate protons. Consistently, the proportion
of the closed state increases with increasing pH. We propose a detailed but straightforward
and robust mechanism comprising a ‘domino effect’ series of proton transfers and
electrostatic interactions: the forward wave (‘dominoes stacking’) primes the
pump, and the reverse wave (‘dominoes falling’) results in the ejection of all
pumped protons from the distal subunit NuoL. This mechanism explains why protons
exit exclusively from the NuoL subunit and is supported by our mutagenesis data.
We contend that this is a universal coupling mechanism of complex I and related
enzymes.'
acknowledged_ssus:
- _id: EM-Fac
- _id: LifeSc
- _id: ScienComp
acknowledgement: This research was supported by the Scientific Service Units (SSU)
of IST Austria through resources provided by the Electron Microscopy Facility (EMF),
the Life Science Facility (LSF) and the IST high-performance computing cluster.
We thank V.-V. Hodirnau from IST Austria EMF, M. Babiak from CEITEC for assistance
with collecting cryo-EM data and A. Charnagalov for the assistance with protein
purification. V.K. was a recipient of a DOC Fellowship of the Austrian Academy of
Sciences at the Institute of Science and Technology, Austria. V.K. and O.P. are
funded by the ERC Advanced Grant 101020697 RESPICHAIN to L.S. This work was also
supported by the Medical Research Council (UK).
article_processing_charge: No
article_type: original
author:
- first_name: Vladyslav
full_name: Kravchuk, Vladyslav
id: 4D62F2A6-F248-11E8-B48F-1D18A9856A87
last_name: Kravchuk
- first_name: Olga
full_name: Petrova, Olga
id: 5D8C9660-5D49-11EA-8188-567B3DDC885E
last_name: Petrova
- first_name: Domen
full_name: Kampjut, Domen
id: 37233050-F248-11E8-B48F-1D18A9856A87
last_name: Kampjut
- first_name: Anna
full_name: Wojciechowska-Bason, Anna
last_name: Wojciechowska-Bason
- first_name: Zara
full_name: Breese, Zara
last_name: Breese
- first_name: Leonid A
full_name: Sazanov, Leonid A
id: 338D39FE-F248-11E8-B48F-1D18A9856A87
last_name: Sazanov
orcid: 0000-0002-0977-7989
citation:
ama: Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov
LA. A universal coupling mechanism of respiratory complex I. Nature. 2022;609(7928):808-814.
doi:10.1038/s41586-022-05199-7
apa: Kravchuk, V., Petrova, O., Kampjut, D., Wojciechowska-Bason, A., Breese, Z.,
& Sazanov, L. A. (2022). A universal coupling mechanism of respiratory complex
I. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05199-7
chicago: Kravchuk, Vladyslav, Olga Petrova, Domen Kampjut, Anna Wojciechowska-Bason,
Zara Breese, and Leonid A Sazanov. “A Universal Coupling Mechanism of Respiratory
Complex I.” Nature. Springer Nature, 2022. https://doi.org/10.1038/s41586-022-05199-7.
ieee: V. Kravchuk, O. Petrova, D. Kampjut, A. Wojciechowska-Bason, Z. Breese, and
L. A. Sazanov, “A universal coupling mechanism of respiratory complex I,” Nature,
vol. 609, no. 7928. Springer Nature, pp. 808–814, 2022.
ista: Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov
LA. 2022. A universal coupling mechanism of respiratory complex I. Nature. 609(7928),
808–814.
mla: Kravchuk, Vladyslav, et al. “A Universal Coupling Mechanism of Respiratory
Complex I.” Nature, vol. 609, no. 7928, Springer Nature, 2022, pp. 808–14,
doi:10.1038/s41586-022-05199-7.
short: V. Kravchuk, O. Petrova, D. Kampjut, A. Wojciechowska-Bason, Z. Breese, L.A.
Sazanov, Nature 609 (2022) 808–814.
date_created: 2023-01-12T12:04:33Z
date_published: 2022-09-22T00:00:00Z
date_updated: 2023-08-04T08:54:52Z
day: '22'
ddc:
- '572'
department:
- _id: LeSa
doi: 10.1038/s41586-022-05199-7
ec_funded: 1
external_id:
isi:
- '000854788200001'
pmid:
- '36104567'
file:
- access_level: open_access
checksum: d42a93e24f59e883ef0b5429832391d0
content_type: application/pdf
creator: lsazanov
date_created: 2023-05-30T17:05:31Z
date_updated: 2023-05-30T17:05:31Z
file_id: '13104'
file_name: EcCxI_manuscript_rev3_noSI_updated_withFigs_opt.pdf
file_size: 1425655
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creator: lsazanov
date_created: 2023-05-30T17:07:05Z
date_updated: 2023-05-30T17:07:05Z
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file_name: EcCxI_manuscript_rev3_SI_All_opt_upd.pdf
file_size: 9842513
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success: 1
file_date_updated: 2023-05-30T17:07:05Z
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intvolume: ' 609'
isi: 1
issue: '7928'
keyword:
- Multidisciplinary
language:
- iso: eng
month: '09'
oa: 1
oa_version: Submitted Version
page: 808-814
pmid: 1
project:
- _id: 238A0A5A-32DE-11EA-91FC-C7463DDC885E
grant_number: '25541'
name: 'Structural characterization of E. coli complex I: an important mechanistic
model'
- _id: 627abdeb-2b32-11ec-9570-ec31a97243d3
call_identifier: H2020
grant_number: '101020697'
name: Structure and mechanism of respiratory chain molecular machines
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1038/s41586-022-05457-8
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/proton-dominos-kick-off-life/
record:
- id: '12781'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: A universal coupling mechanism of respiratory complex I
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 609
year: '2022'
...
---
_id: '12144'
abstract:
- lang: eng
text: The phytohormone auxin is the major coordinative signal in plant development1,
mediating transcriptional reprogramming by a well-established canonical signalling
pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin
receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin,
they associate with Aux/IAA transcriptional repressors and target them for degradation
via ubiquitination2,3. Here we identify adenylate cyclase (AC) activity as an
additional function of TIR1/AFB receptors across land plants. Auxin, together
with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC
motif of the TIR1 C-terminal region, all of which abolish the AC activity, each
render TIR1 ineffective in mediating gravitropism and sustained auxin-induced
root growth inhibition, and also affect auxin-induced transcriptional regulation.
These results highlight the importance of TIR1/AFB AC activity in canonical auxin
signalling. They also identify a unique phytohormone receptor cassette combining
F-box and AC motifs, and the role of cAMP as a second messenger in plants.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: This research was supported by the Lab Support Facility (LSF) and
the Imaging and Optics Facility (IOF) of IST Austria. We thank C. Gehring for suggestions
and advice; and K. U. Torii and G. Stacey for seeds and plasmids. This project was
funded by a European Research Council Advanced Grant (ETAP-742985). M.F.K. and R.N.
acknowledge the support of the EU MSCA-IF project CrysPINs (792329). M.K. was supported
by the project POWR.03.05.00-00-Z302/17 Universitas Copernicana Thoruniensis in
Futuro–IDS “Academia Copernicana”. CIDG acknowledges support from UKRI under Future
Leaders Fellowship grant number MR/T020652/1.
article_processing_charge: No
article_type: original
author:
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Mateusz
full_name: Kwiatkowski, Mateusz
last_name: Kwiatkowski
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Scott A
full_name: Sinclair, Scott A
id: 2D99FE6A-F248-11E8-B48F-1D18A9856A87
last_name: Sinclair
orcid: 0000-0002-4566-0593
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Charo I.
full_name: del Genio, Charo I.
last_name: del Genio
- first_name: Martin F.
full_name: Kubeš, Martin F.
last_name: Kubeš
- first_name: Richard
full_name: Napier, Richard
last_name: Napier
- first_name: Krzysztof
full_name: Jaworski, Krzysztof
last_name: Jaworski
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Qi L, Kwiatkowski M, Chen H, et al. Adenylate cyclase activity of TIR1/AFB
auxin receptors in plants. Nature. 2022;611(7934):133-138. doi:10.1038/s41586-022-05369-7
apa: Qi, L., Kwiatkowski, M., Chen, H., Hörmayer, L., Sinclair, S. A., Zou, M.,
… Friml, J. (2022). Adenylate cyclase activity of TIR1/AFB auxin receptors in
plants. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05369-7
chicago: Qi, Linlin, Mateusz Kwiatkowski, Huihuang Chen, Lukas Hörmayer, Scott A
Sinclair, Minxia Zou, Charo I. del Genio, et al. “Adenylate Cyclase Activity of
TIR1/AFB Auxin Receptors in Plants.” Nature. Springer Nature, 2022. https://doi.org/10.1038/s41586-022-05369-7.
ieee: L. Qi et al., “Adenylate cyclase activity of TIR1/AFB auxin receptors
in plants,” Nature, vol. 611, no. 7934. Springer Nature, pp. 133–138, 2022.
ista: Qi L, Kwiatkowski M, Chen H, Hörmayer L, Sinclair SA, Zou M, del Genio CI,
Kubeš MF, Napier R, Jaworski K, Friml J. 2022. Adenylate cyclase activity of TIR1/AFB
auxin receptors in plants. Nature. 611(7934), 133–138.
mla: Qi, Linlin, et al. “Adenylate Cyclase Activity of TIR1/AFB Auxin Receptors
in Plants.” Nature, vol. 611, no. 7934, Springer Nature, 2022, pp. 133–38,
doi:10.1038/s41586-022-05369-7.
short: L. Qi, M. Kwiatkowski, H. Chen, L. Hörmayer, S.A. Sinclair, M. Zou, C.I.
del Genio, M.F. Kubeš, R. Napier, K. Jaworski, J. Friml, Nature 611 (2022) 133–138.
date_created: 2023-01-12T12:06:05Z
date_published: 2022-11-03T00:00:00Z
date_updated: 2023-10-03T11:04:53Z
day: '03'
department:
- _id: JiFr
doi: 10.1038/s41586-022-05369-7
ec_funded: 1
external_id:
isi:
- '000875061600013'
pmid:
- '36289340'
intvolume: ' 611'
isi: 1
issue: '7934'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://wrap.warwick.ac.uk/168325/1/WRAP-denylate-cyclase-activity-TIR1-AFB-auxin-receptors-root-growth-22.pdf
month: '11'
oa: 1
oa_version: Submitted Version
page: 133-138
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adenylate cyclase activity of TIR1/AFB auxin receptors in plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 611
year: '2022'
...
---
_id: '12274'
abstract:
- lang: eng
text: The morphology and functionality of the epithelial lining differ along the
intestinal tract, but tissue renewal at all sites is driven by stem cells at the
base of crypts1,2,3. Whether stem cell numbers and behaviour vary at different
sites is unknown. Here we show using intravital microscopy that, despite similarities
in the number and distribution of proliferative cells with an Lgr5 signature in
mice, small intestinal crypts contain twice as many effective stem cells as large
intestinal crypts. We find that, although passively displaced by a conveyor-belt-like
upward movement, small intestinal cells positioned away from the crypt base can
function as long-term effective stem cells owing to Wnt-dependent retrograde cellular
movement. By contrast, the near absence of retrograde movement in the large intestine
restricts cell repositioning, leading to a reduction in effective stem cell number.
Moreover, after suppression of the retrograde movement in the small intestine,
the number of effective stem cells is reduced, and the rate of monoclonal conversion
of crypts is accelerated. Together, these results show that the number of effective
stem cells is determined by active retrograde movement, revealing a new channel
of stem cell regulation that can be experimentally and pharmacologically manipulated.
acknowledgement: We thank the members of the van Rheenen laboratory for reading the
manuscript, and the members of the bioimaging, FACS and animal facility of the NKI
for experimental support. We acknowledge the staff at the MedH Flow Cytometry core
facility, Karolinska Institutet, and LCI facility/Nikon Center of Excellence, Karolinska
Institutet. This work was financially supported by the Netherlands Organization
of Scientific Research NWO (Veni grant 863.15.011 to S.I.J.E. and Vici grant 09150182110004
to J.v.R.) and the CancerGenomics.nl (Netherlands Organisation for Scientific Research)
program (to J.v.R.) the Doctor Josef Steiner Foundation (to J.v.R). B.D.S. acknowledges
funding from the Royal Society E.P. Abraham Research Professorship (RP\R1\180165)
and the Wellcome Trust (098357/Z/12/Z and 219478/Z/19/Z). B.C.-M. acknowledges the
support of the field of excellence ‘Complexity of life in basic research and innovation’
of the University of Graz. O.J.S. and their laboratory acknowledge CRUK core funding
to the CRUK Beatson Institute (A17196 and A31287) and CRUK core funding to the Sansom
laboratory (A21139). P.K. and their laboratory are supported by grants from the
Swedish Research Council (2018-03078), Cancerfonden (190634), Academy of Finland
Centre of Excellence (266869, 304591 and 320185) and the Jane and Aatos Erkko Foundation.
P.L. has received funding from the European Research Council (ERC) under the European
Union’s Horizon 2020 research and innovation programme (grant agreement no. 758617).
E.H. acknowledges funding from the European Research Council (ERC) under the European
Union’s Horizon 2020 research and innovation programme (grant agreement no. 851288).
article_processing_charge: No
article_type: original
author:
- first_name: Maria
full_name: Azkanaz, Maria
last_name: Azkanaz
- first_name: Bernat
full_name: Corominas-Murtra, Bernat
id: 43BE2298-F248-11E8-B48F-1D18A9856A87
last_name: Corominas-Murtra
orcid: 0000-0001-9806-5643
- first_name: Saskia I. J.
full_name: Ellenbroek, Saskia I. J.
last_name: Ellenbroek
- first_name: Lotte
full_name: Bruens, Lotte
last_name: Bruens
- first_name: Anna T.
full_name: Webb, Anna T.
last_name: Webb
- first_name: Dimitrios
full_name: Laskaris, Dimitrios
last_name: Laskaris
- first_name: Koen C.
full_name: Oost, Koen C.
last_name: Oost
- first_name: Simona J. A.
full_name: Lafirenze, Simona J. A.
last_name: Lafirenze
- first_name: Karl
full_name: Annusver, Karl
last_name: Annusver
- first_name: Hendrik A.
full_name: Messal, Hendrik A.
last_name: Messal
- first_name: Sharif
full_name: Iqbal, Sharif
last_name: Iqbal
- first_name: Dustin J.
full_name: Flanagan, Dustin J.
last_name: Flanagan
- first_name: David J.
full_name: Huels, David J.
last_name: Huels
- first_name: Felipe
full_name: Rojas-Rodríguez, Felipe
last_name: Rojas-Rodríguez
- first_name: Miguel
full_name: Vizoso, Miguel
last_name: Vizoso
- first_name: Maria
full_name: Kasper, Maria
last_name: Kasper
- first_name: Owen J.
full_name: Sansom, Owen J.
last_name: Sansom
- first_name: Hugo J.
full_name: Snippert, Hugo J.
last_name: Snippert
- first_name: Prisca
full_name: Liberali, Prisca
last_name: Liberali
- first_name: Benjamin D.
full_name: Simons, Benjamin D.
last_name: Simons
- first_name: Pekka
full_name: Katajisto, Pekka
last_name: Katajisto
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Jacco
full_name: van Rheenen, Jacco
last_name: van Rheenen
citation:
ama: Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, et al. Retrograde movements
determine effective stem cell numbers in the intestine. Nature. 2022;607(7919):548-554.
doi:10.1038/s41586-022-04962-0
apa: Azkanaz, M., Corominas-Murtra, B., Ellenbroek, S. I. J., Bruens, L., Webb,
A. T., Laskaris, D., … van Rheenen, J. (2022). Retrograde movements determine
effective stem cell numbers in the intestine. Nature. Springer Nature.
https://doi.org/10.1038/s41586-022-04962-0
chicago: Azkanaz, Maria, Bernat Corominas-Murtra, Saskia I. J. Ellenbroek, Lotte
Bruens, Anna T. Webb, Dimitrios Laskaris, Koen C. Oost, et al. “Retrograde Movements
Determine Effective Stem Cell Numbers in the Intestine.” Nature. Springer
Nature, 2022. https://doi.org/10.1038/s41586-022-04962-0.
ieee: M. Azkanaz et al., “Retrograde movements determine effective stem cell
numbers in the intestine,” Nature, vol. 607, no. 7919. Springer Nature,
pp. 548–554, 2022.
ista: Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, Bruens L, Webb AT, Laskaris
D, Oost KC, Lafirenze SJA, Annusver K, Messal HA, Iqbal S, Flanagan DJ, Huels
DJ, Rojas-Rodríguez F, Vizoso M, Kasper M, Sansom OJ, Snippert HJ, Liberali P,
Simons BD, Katajisto P, Hannezo EB, van Rheenen J. 2022. Retrograde movements
determine effective stem cell numbers in the intestine. Nature. 607(7919), 548–554.
mla: Azkanaz, Maria, et al. “Retrograde Movements Determine Effective Stem Cell
Numbers in the Intestine.” Nature, vol. 607, no. 7919, Springer Nature,
2022, pp. 548–54, doi:10.1038/s41586-022-04962-0.
short: M. Azkanaz, B. Corominas-Murtra, S.I.J. Ellenbroek, L. Bruens, A.T. Webb,
D. Laskaris, K.C. Oost, S.J.A. Lafirenze, K. Annusver, H.A. Messal, S. Iqbal,
D.J. Flanagan, D.J. Huels, F. Rojas-Rodríguez, M. Vizoso, M. Kasper, O.J. Sansom,
H.J. Snippert, P. Liberali, B.D. Simons, P. Katajisto, E.B. Hannezo, J. van Rheenen,
Nature 607 (2022) 548–554.
date_created: 2023-01-16T10:01:29Z
date_published: 2022-07-13T00:00:00Z
date_updated: 2023-10-03T11:16:30Z
day: '13'
department:
- _id: EdHa
doi: 10.1038/s41586-022-04962-0
ec_funded: 1
external_id:
isi:
- '000824430000004'
pmid:
- '35831497'
intvolume: ' 607'
isi: 1
issue: '7919'
keyword:
- Multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://helda.helsinki.fi/items/94433455-4854-45c0-9de8-7326caea8780
month: '07'
oa: 1
oa_version: Submitted Version
page: 548-554
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/JaccovanRheenenLab/Retrograde_movement_Azkanaz_Nature_2022
scopus_import: '1'
status: public
title: Retrograde movements determine effective stem cell numbers in the intestine
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 607
year: '2022'
...
---
_id: '12291'
abstract:
- lang: eng
text: The phytohormone auxin triggers transcriptional reprogramming through a well-characterized
perception machinery in the nucleus. By contrast, mechanisms that underlie fast
effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation
of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding
protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4.
Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds
auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its
plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required
for the auxin-induced ultrafast global phospho-response and for downstream processes
that include the activation of H+-ATPase and accelerated cytoplasmic streaming.
abp1 and tmk mutants cannot establish auxin-transporting channels and show defective
auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that
lacks the capacity to bind auxin is unable to complement these defects in abp1
mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface
signalling, which mediates the global phospho-response and auxin canalization.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: We acknowledge K. Kubiasová for excellent technical assistance, J.
Neuhold, A. Lehner and A. Sedivy for technical assistance with protein production
and purification at Vienna Biocenter Core Facilities; Creoptix for performing GCI;
and the Bioimaging, Electron Microscopy and Life Science Facilities at ISTA, the
Plant Sciences Core Facility of CEITEC Masaryk University, the Core Facility CELLIM
(MEYS CR, LM2018129 Czech-BioImaging) and J. Sprakel for their assistance. J.F.
is grateful to R. Napier for many insightful suggestions and support. We thank all
past and present members of the Friml group for their support and for other contributions
to this effort to clarify the controversial role of ABP1 over the past seven years.
The project received funding from the European Research Council (ERC) under the
European Union’s Horizon 2020 research and innovation program (grant agreement no.
742985 to J.F. and 833867 to D.W.); the Austrian Science Fund (FWF; P29988 to J.F.);
the Netherlands Organization for Scientific Research (NWO; VICI grant 865.14.001
to D.W. and VENI grant VI.Veni.212.003 to A.K.); the Ministry of Education, Science
and Technological Development of the Republic of Serbia (contract no. 451-03-68/2022-14/200053
to B.D.Ž.); and the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and
20H05910).
article_processing_charge: No
article_type: original
author:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Mark
full_name: Roosjen, Mark
last_name: Roosjen
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Branka D.
full_name: Živanović, Branka D.
last_name: Živanović
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Peter
full_name: Grones, Peter
last_name: Grones
- first_name: Mónika
full_name: Hrtyan, Mónika
id: 45A71A74-F248-11E8-B48F-1D18A9856A87
last_name: Hrtyan
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Andre
full_name: Kuhn, Andre
last_name: Kuhn
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Marek
full_name: Randuch, Marek
id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae
last_name: Randuch
- first_name: Nikola
full_name: Rýdza, Nikola
last_name: Rýdza
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Anastasiia
full_name: Teplova, Anastasiia
id: e3736151-106c-11ec-b916-c2558e2762c6
last_name: Teplova
- first_name: Toshinori
full_name: Kinoshita, Toshinori
last_name: Kinoshita
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
citation:
ama: Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation
and auxin canalization. Nature. 2022;609(7927):575-581. doi:10.1038/s41586-022-05187-x
apa: Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A.,
… Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and
auxin canalization. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05187-x
chicago: Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa
Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception
for Global Phosphorylation and Auxin Canalization.” Nature. Springer Nature,
2022. https://doi.org/10.1038/s41586-022-05187-x.
ieee: J. Friml et al., “ABP1–TMK auxin perception for global phosphorylation
and auxin canalization,” Nature, vol. 609, no. 7927. Springer Nature, pp.
575–581, 2022.
ista: Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey
L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones
P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K,
Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception
for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581.
mla: Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and
Auxin Canalization.” Nature, vol. 609, no. 7927, Springer Nature, 2022,
pp. 575–81, doi:10.1038/s41586-022-05187-x.
short: J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez
Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini,
P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza,
K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature
609 (2022) 575–581.
date_created: 2023-01-16T10:04:48Z
date_published: 2022-09-15T00:00:00Z
date_updated: 2023-11-07T08:16:09Z
day: '15'
ddc:
- '580'
department:
- _id: JiFr
- _id: GradSch
- _id: EvBe
- _id: EM-Fac
doi: 10.1038/s41586-022-05187-x
ec_funded: 1
external_id:
isi:
- '000851357500002'
pmid:
- '36071161'
file:
- access_level: open_access
checksum: a6055c606aefb900bf62ae3e7d15f921
content_type: application/pdf
creator: amally
date_created: 2023-11-02T17:12:37Z
date_updated: 2023-11-02T17:12:37Z
file_id: '14483'
file_name: Friml Nature 2022_merged.pdf
file_size: 79774945
relation: main_file
success: 1
file_date_updated: 2023-11-02T17:12:37Z
has_accepted_license: '1'
intvolume: ' 609'
isi: 1
issue: '7927'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Submitted Version
page: 575-581
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: ABP1–TMK auxin perception for global phosphorylation and auxin canalization
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 609
year: '2022'
...
---
_id: '12671'
abstract:
- lang: eng
text: Sperm chromatin is typically transformed by protamines into a compact and
transcriptionally inactive state1,2. Sperm cells of flowering plants lack protamines,
yet they have small, transcriptionally active nuclei with chromatin condensed
through an unknown mechanism3,4. Here we show that a histone variant, H2B.8, mediates
sperm chromatin and nuclear condensation in Arabidopsis thaliana. Loss of H2B.8
causes enlarged sperm nuclei with dispersed chromatin, whereas ectopic expression
in somatic cells produces smaller nuclei with aggregated chromatin. This result
demonstrates that H2B.8 is sufficient for chromatin condensation. H2B.8 aggregates
transcriptionally inactive AT-rich chromatin into phase-separated condensates,
which facilitates nuclear compaction without reducing transcription. Reciprocal
crosses show that mutation of h2b.8 reduces male transmission, which suggests
that H2B.8-mediated sperm compaction is important for fertility. Altogether, our
results reveal a new mechanism of nuclear compaction through global aggregation
of unexpressed chromatin. We propose that H2B.8 is an evolutionary innovation
of flowering plants that achieves nuclear condensation compatible with active
transcription.
article_processing_charge: No
article_type: original
author:
- first_name: Toby
full_name: Buttress, Toby
last_name: Buttress
- first_name: Shengbo
full_name: He, Shengbo
last_name: He
- first_name: Liang
full_name: Wang, Liang
last_name: Wang
- first_name: Shaoli
full_name: Zhou, Shaoli
last_name: Zhou
- first_name: Gerhard
full_name: Saalbach, Gerhard
last_name: Saalbach
- first_name: Martin
full_name: Vickers, Martin
last_name: Vickers
- first_name: Guohong
full_name: Li, Guohong
last_name: Li
- first_name: Pilong
full_name: Li, Pilong
last_name: Li
- first_name: Xiaoqi
full_name: Feng, Xiaoqi
id: e0164712-22ee-11ed-b12a-d80fcdf35958
last_name: Feng
orcid: 0000-0002-4008-1234
citation:
ama: Buttress T, He S, Wang L, et al. Histone H2B.8 compacts flowering plant sperm
through chromatin phase separation. Nature. 2022;611(7936):614-622. doi:10.1038/s41586-022-05386-6
apa: Buttress, T., He, S., Wang, L., Zhou, S., Saalbach, G., Vickers, M., … Feng,
X. (2022). Histone H2B.8 compacts flowering plant sperm through chromatin phase
separation. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05386-6
chicago: Buttress, Toby, Shengbo He, Liang Wang, Shaoli Zhou, Gerhard Saalbach,
Martin Vickers, Guohong Li, Pilong Li, and Xiaoqi Feng. “Histone H2B.8 Compacts
Flowering Plant Sperm through Chromatin Phase Separation.” Nature. Springer
Nature, 2022. https://doi.org/10.1038/s41586-022-05386-6.
ieee: T. Buttress et al., “Histone H2B.8 compacts flowering plant sperm through
chromatin phase separation,” Nature, vol. 611, no. 7936. Springer Nature,
pp. 614–622, 2022.
ista: Buttress T, He S, Wang L, Zhou S, Saalbach G, Vickers M, Li G, Li P, Feng
X. 2022. Histone H2B.8 compacts flowering plant sperm through chromatin phase
separation. Nature. 611(7936), 614–622.
mla: Buttress, Toby, et al. “Histone H2B.8 Compacts Flowering Plant Sperm through
Chromatin Phase Separation.” Nature, vol. 611, no. 7936, Springer Nature,
2022, pp. 614–22, doi:10.1038/s41586-022-05386-6.
short: T. Buttress, S. He, L. Wang, S. Zhou, G. Saalbach, M. Vickers, G. Li, P.
Li, X. Feng, Nature 611 (2022) 614–622.
date_created: 2023-02-23T09:17:05Z
date_published: 2022-11-17T00:00:00Z
date_updated: 2023-05-08T10:59:22Z
day: '17'
department:
- _id: XiFe
doi: 10.1038/s41586-022-05386-6
extern: '1'
external_id:
pmid:
- '36323776'
intvolume: ' 611'
issue: '7936'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41586-022-05386-6
month: '11'
oa: 1
oa_version: Published Version
page: 614-622
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Histone H2B.8 compacts flowering plant sperm through chromatin phase separation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 611
year: '2022'
...
---
_id: '9549'
abstract:
- lang: eng
text: 'AMPA receptors (AMPARs) mediate the majority of excitatory transmission in
the brain and enable the synaptic plasticity that underlies learning1. A diverse
array of AMPAR signalling complexes are established by receptor auxiliary subunits,
which associate with the AMPAR in various combinations to modulate trafficking,
gating and synaptic strength2. However, their mechanisms of action are poorly
understood. Here we determine cryo-electron microscopy structures of the heteromeric
GluA1–GluA2 receptor assembled with both TARP-γ8 and CNIH2, the predominant AMPAR
complex in the forebrain, in both resting and active states. Two TARP-γ8 and two
CNIH2 subunits insert at distinct sites beneath the ligand-binding domains of
the receptor, with site-specific lipids shaping each interaction and affecting
the gating regulation of the AMPARs. Activation of the receptor leads to asymmetry
between GluA1 and GluA2 along the ion conduction path and an outward expansion
of the channel triggers counter-rotations of both auxiliary subunit pairs, promoting
the active-state conformation. In addition, both TARP-γ8 and CNIH2 pivot towards
the pore exit upon activation, extending their reach for cytoplasmic receptor
elements. CNIH2 achieves this through its uniquely extended M2 helix, which has
transformed this endoplasmic reticulum-export factor into a powerful AMPAR modulator
that is capable of providing hippocampal pyramidal neurons with their integrative
synaptic properties. '
acknowledgement: We thank members of the Greger laboratory, B. Herguedas, J. Krieger
and J.-N. Dohrke for comments on the manuscript; J. Krieger and J.-N. Dohrke for
discussion, J. Krieger for help with the normal mode analysis, B. Köhegyi for help
with cryo-EM imaging, V. Chang and K. Suzuki for helping to generate the CNIH2-1D4-HA
stable cell line, M. Carvalho for assistance at early stages of this project, the
LMB scientific computing and the cryo-EM facility for support, P. Emsley for help
with model building, T. Nakane for helpful comments with RELION 3.1 and R. Warshamanage
for helping with EMDA cryo-EM-map processing. We acknowledge the Diamond Light Source
for access and support of the Cryo-EM facilities at the UK national electron bio10
imaging centre (eBIC), proposal EM17434, funded by the Wellcome Trust, MRC and BBSRC.
This work was supported by grants from the Medical Research Council, as part of
United Kingdom Research and Innovation (also known as UK Research and Innovation)
(MC_U105174197) and BBSRC (BB/N002113/1) to I.H.G.
article_processing_charge: No
article_type: original
author:
- first_name: Danyang
full_name: Zhang, Danyang
last_name: Zhang
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Peter M.
full_name: Matthews, Peter M.
last_name: Matthews
- first_name: Ondrej
full_name: Cais, Ondrej
last_name: Cais
- first_name: Ingo H.
full_name: Greger, Ingo H.
last_name: Greger
citation:
ama: Zhang D, Watson J, Matthews PM, Cais O, Greger IH. Gating and modulation of
a hetero-octameric AMPA glutamate receptor. Nature. 2021;594:454-458. doi:10.1038/s41586-021-03613-0
apa: Zhang, D., Watson, J., Matthews, P. M., Cais, O., & Greger, I. H. (2021).
Gating and modulation of a hetero-octameric AMPA glutamate receptor. Nature.
Springer Nature. https://doi.org/10.1038/s41586-021-03613-0
chicago: Zhang, Danyang, Jake Watson, Peter M. Matthews, Ondrej Cais, and Ingo H.
Greger. “Gating and Modulation of a Hetero-Octameric AMPA Glutamate Receptor.”
Nature. Springer Nature, 2021. https://doi.org/10.1038/s41586-021-03613-0.
ieee: D. Zhang, J. Watson, P. M. Matthews, O. Cais, and I. H. Greger, “Gating and
modulation of a hetero-octameric AMPA glutamate receptor,” Nature, vol.
594. Springer Nature, pp. 454–458, 2021.
ista: Zhang D, Watson J, Matthews PM, Cais O, Greger IH. 2021. Gating and modulation
of a hetero-octameric AMPA glutamate receptor. Nature. 594, 454–458.
mla: Zhang, Danyang, et al. “Gating and Modulation of a Hetero-Octameric AMPA Glutamate
Receptor.” Nature, vol. 594, Springer Nature, 2021, pp. 454–58, doi:10.1038/s41586-021-03613-0.
short: D. Zhang, J. Watson, P.M. Matthews, O. Cais, I.H. Greger, Nature 594 (2021)
454–458.
date_created: 2021-06-13T22:01:33Z
date_published: 2021-06-02T00:00:00Z
date_updated: 2023-08-08T13:59:51Z
day: '02'
department:
- _id: PeJo
doi: 10.1038/s41586-021-03613-0
external_id:
isi:
- '000657238100003'
pmid:
- '34079129'
intvolume: ' 594'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41586-021-03613-0
month: '06'
oa: 1
oa_version: Published Version
page: 454-458
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Gating and modulation of a hetero-octameric AMPA glutamate receptor
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 594
year: '2021'
...
---
_id: '10025'
abstract:
- lang: eng
text: Ferromagnetism is most common in transition metal compounds but may also arise
in low-density two-dimensional electron systems, with signatures observed in silicon,
III-V semiconductor systems, and graphene moiré heterostructures. Here we show
that gate-tuned van Hove singularities in rhombohedral trilayer graphene drive
the spontaneous ferromagnetic polarization of the electron system into one or
more spin- and valley flavors. Using capacitance measurements on graphite-gated
van der Waals heterostructures, we find a cascade of density- and electronic displacement
field tuned phase transitions marked by negative electronic compressibility. The
transitions define the boundaries between phases where quantum oscillations have
either four-fold, two-fold, or one-fold degeneracy, associated with a spin and
valley degenerate normal metal, spin-polarized `half-metal', and spin and valley
polarized `quarter metal', respectively. For electron doping, the salient features
are well captured by a phenomenological Stoner model with a valley-anisotropic
Hund's coupling, likely arising from interactions at the lattice scale. For hole
filling, we observe a richer phase diagram featuring a delicate interplay of broken
symmetries and transitions in the Fermi surface topology. Finally, by rotational
alignment of a hexagonal boron nitride substrate to induce a moiré superlattice,
we find that the superlattice perturbs the preexisting isospin order only weakly,
leaving the basic phase diagram intact while catalyzing the formation of topologically
nontrivial gapped states whenever itinerant half- or quarter metal states occur
at half- or quarter superlattice band filling. Our results show that rhombohedral
trilayer graphene is an ideal platform for well-controlled tests of many-body
theory and reveal magnetism in moiré materials to be fundamentally itinerant in
nature.
acknowledgement: "The authors acknowledge discussions with A. Macdonald, L. Fu, F.
Wang and M. Zaletel. AFY acknowledges support of the National Science Foundation
under DMR1654186, and the Gordon and Betty Moore Foundation under award GBMF9471.
The authors acknowledge the use of the research facilities within the California
NanoSystems Institute, supported by the University of California, Santa Barbara
and the University of California, Office of the President.\r\nK.W. and T.T. acknowledge
support from the Elemental Strategy Initiative conducted by the MEXT, Japan, Grant
Number JPMXP0112101001 and JSPS KAKENHI, Grant Number JP20H00354. EB and TH were
supported by the European Research Council (ERC) under grant HQMAT (Grant Agreement
No. 817799). A.G. acknowledges support by the European Unions Horizon 2020 research
and innovation program under the Marie Sklodowska-Curie Grant Agreement\r\nNo. 754411.\r\n"
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Haoxin
full_name: Zhou, Haoxin
last_name: Zhou
- first_name: Tian
full_name: Xie, Tian
last_name: Xie
- first_name: Areg
full_name: Ghazaryan, Areg
id: 4AF46FD6-F248-11E8-B48F-1D18A9856A87
last_name: Ghazaryan
orcid: 0000-0001-9666-3543
- first_name: Tobias
full_name: Holder, Tobias
last_name: Holder
- first_name: James R.
full_name: Ehrets, James R.
last_name: Ehrets
- first_name: Eric M.
full_name: Spanton, Eric M.
last_name: Spanton
- first_name: Takashi
full_name: Taniguchi, Takashi
last_name: Taniguchi
- first_name: Kenji
full_name: Watanabe, Kenji
last_name: Watanabe
- first_name: Erez
full_name: Berg, Erez
last_name: Berg
- first_name: Maksym
full_name: Serbyn, Maksym
id: 47809E7E-F248-11E8-B48F-1D18A9856A87
last_name: Serbyn
orcid: 0000-0002-2399-5827
- first_name: Andrea F.
full_name: Young, Andrea F.
last_name: Young
citation:
ama: Zhou H, Xie T, Ghazaryan A, et al. Half and quarter metals in rhombohedral
trilayer graphene. Nature. 2021. doi:10.1038/s41586-021-03938-w
apa: Zhou, H., Xie, T., Ghazaryan, A., Holder, T., Ehrets, J. R., Spanton, E. M.,
… Young, A. F. (2021). Half and quarter metals in rhombohedral trilayer graphene.
Nature. Springer Nature. https://doi.org/10.1038/s41586-021-03938-w
chicago: Zhou, Haoxin, Tian Xie, Areg Ghazaryan, Tobias Holder, James R. Ehrets,
Eric M. Spanton, Takashi Taniguchi, et al. “Half and Quarter Metals in Rhombohedral
Trilayer Graphene.” Nature. Springer Nature, 2021. https://doi.org/10.1038/s41586-021-03938-w.
ieee: H. Zhou et al., “Half and quarter metals in rhombohedral trilayer graphene,”
Nature. Springer Nature, 2021.
ista: Zhou H, Xie T, Ghazaryan A, Holder T, Ehrets JR, Spanton EM, Taniguchi T,
Watanabe K, Berg E, Serbyn M, Young AF. 2021. Half and quarter metals in rhombohedral
trilayer graphene. Nature.
mla: Zhou, Haoxin, et al. “Half and Quarter Metals in Rhombohedral Trilayer Graphene.”
Nature, Springer Nature, 2021, doi:10.1038/s41586-021-03938-w.
short: H. Zhou, T. Xie, A. Ghazaryan, T. Holder, J.R. Ehrets, E.M. Spanton, T. Taniguchi,
K. Watanabe, E. Berg, M. Serbyn, A.F. Young, Nature (2021).
date_created: 2021-09-19T22:01:25Z
date_published: 2021-09-01T00:00:00Z
date_updated: 2023-08-14T07:04:06Z
day: '01'
department:
- _id: MaSe
- _id: MiLe
doi: 10.1038/s41586-021-03938-w
ec_funded: 1
external_id:
arxiv:
- '2104.00653'
isi:
- '000706977400002'
isi: 1
keyword:
- condensed matter - mesoscale and nanoscale physics
- condensed matter - strongly correlated electrons
- multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/2104.00653
month: '09'
oa: 1
oa_version: Preprint
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1038/s41586-021-04181-z
scopus_import: '1'
status: public
title: Half and quarter metals in rhombohedral trilayer graphene
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
year: '2021'
...
---
_id: '10146'
abstract:
- lang: eng
text: The enzymes of the mitochondrial electron transport chain are key players
of cell metabolism. Despite being active when isolated, in vivo they associate
into supercomplexes1, whose precise role is debated. Supercomplexes CIII2CIV1-2
(refs. 2,3), CICIII2 (ref. 4) and CICIII2CIV (respirasome)5,6,7,8,9,10 exist in
mammals, but in contrast to CICIII2 and the respirasome, to date the only known
eukaryotic structures of CIII2CIV1-2 come from Saccharomyces cerevisiae11,12 and
plants13, which have different organization. Here we present the first, to our
knowledge, structures of mammalian (mouse and ovine) CIII2CIV and its assembly
intermediates, in different conformations. We describe the assembly of CIII2CIV
from the CIII2 precursor to the final CIII2CIV conformation, driven by the insertion
of the N terminus of the assembly factor SCAF1 (ref. 14) deep into CIII2, while
its C terminus is integrated into CIV. Our structures (which include CICIII2 and
the respirasome) also confirm that SCAF1 is exclusively required for the assembly
of CIII2CIV and has no role in the assembly of the respirasome. We show that CIII2
is asymmetric due to the presence of only one copy of subunit 9, which straddles
both monomers and prevents the attachment of a second copy of SCAF1 to CIII2,
explaining the presence of one copy of CIV in CIII2CIV in mammals. Finally, we
show that CIII2 and CIV gain catalytic advantage when assembled into the supercomplex
and propose a role for CIII2CIV in fine tuning the efficiency of electron transfer
in the electron transport chain.
acknowledged_ssus:
- _id: PreCl
- _id: EM-Fac
- _id: ScienComp
acknowledgement: We thank the pre-clinical facility of the IST Austria and A. Venturino
for assistance with the animals; and V.-V. Hodirnau for assistance during the Titan
Krios data collection, performed at the IST Austria. The data processing was performed
at the IST high-performance computing cluster. This project has received funding
from the European Union’s Horizon 2020 research and innovation program under the
Marie Skłodowska-Curie grant agreement no. 754411.
article_processing_charge: No
article_type: original
author:
- first_name: Irene
full_name: Vercellino, Irene
id: 3ED6AF16-F248-11E8-B48F-1D18A9856A87
last_name: Vercellino
orcid: 0000-0001-5618-3449
- first_name: Leonid A
full_name: Sazanov, Leonid A
id: 338D39FE-F248-11E8-B48F-1D18A9856A87
last_name: Sazanov
orcid: 0000-0002-0977-7989
citation:
ama: Vercellino I, Sazanov LA. Structure and assembly of the mammalian mitochondrial
supercomplex CIII2CIV. Nature. 2021;598(7880):364-367. doi:10.1038/s41586-021-03927-z
apa: Vercellino, I., & Sazanov, L. A. (2021). Structure and assembly of the
mammalian mitochondrial supercomplex CIII2CIV. Nature. Springer
Nature. https://doi.org/10.1038/s41586-021-03927-z
chicago: Vercellino, Irene, and Leonid A Sazanov. “Structure and Assembly of the
Mammalian Mitochondrial Supercomplex CIII2CIV.” Nature. Springer
Nature, 2021. https://doi.org/10.1038/s41586-021-03927-z.
ieee: I. Vercellino and L. A. Sazanov, “Structure and assembly of the mammalian
mitochondrial supercomplex CIII2CIV,” Nature, vol. 598, no.
7880. Springer Nature, pp. 364–367, 2021.
ista: Vercellino I, Sazanov LA. 2021. Structure and assembly of the mammalian mitochondrial
supercomplex CIII2CIV. Nature. 598(7880), 364–367.
mla: Vercellino, Irene, and Leonid A. Sazanov. “Structure and Assembly of the Mammalian
Mitochondrial Supercomplex CIII2CIV.” Nature, vol. 598, no.
7880, Springer Nature, 2021, pp. 364–67, doi:10.1038/s41586-021-03927-z.
short: I. Vercellino, L.A. Sazanov, Nature 598 (2021) 364–367.
date_created: 2021-10-17T22:01:17Z
date_published: 2021-10-14T00:00:00Z
date_updated: 2023-08-14T08:01:21Z
day: '14'
department:
- _id: LeSa
doi: 10.1038/s41586-021-03927-z
ec_funded: 1
external_id:
isi:
- '000704581600001'
pmid:
- '34616041'
intvolume: ' 598'
isi: 1
issue: '7880'
language:
- iso: eng
month: '10'
oa_version: None
page: 364-367
pmid: 1
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Webpage
relation: press_release
url: https://ist.ac.at/en/news/boosting-the-cells-power-house/
scopus_import: '1'
status: public
title: Structure and assembly of the mammalian mitochondrial supercomplex CIII2CIV
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 598
year: '2021'
...
---
_id: '9059'
abstract:
- lang: eng
text: 'From rock salt to nanoparticle superlattices, complex structure can emerge
from simple building blocks that attract each other through Coulombic forces1-4.
On the micrometre scale, however, colloids in water defy the intuitively simple
idea of forming crystals from oppositely charged partners, instead forming non-equilibrium
structures such as clusters and gels5-7. Although various systems have been engineered
to grow binary crystals8-11, native surface charge in aqueous conditions has not
been used to assemble crystalline materials. Here we form ionic colloidal crystals
in water through an approach that we refer to as polymer-attenuated Coulombic
self-assembly. The key to crystallization is the use of a neutral polymer to keep
particles separated by well defined distances, allowing us to tune the attractive
overlap of electrical double layers, directing particles to disperse, crystallize
or become permanently fixed on demand. The nucleation and growth of macroscopic
single crystals is demonstrated by using the Debye screening length to fine-tune
assembly. Using a variety of colloidal particles and commercial polymers, ionic
colloidal crystals isostructural to caesium chloride, sodium chloride, aluminium
diboride and K4C60 are selected according to particle size ratios. Once fixed
by simply diluting out solution salts, crystals are pulled out of the water for
further manipulation, demonstrating an accurate translation from solution-phase
assembly to dried solid structures. In contrast to other assembly approaches,
in which particles must be carefully engineered to encode binding information12-18,
polymer-attenuated Coulombic self-assembly enables conventional colloids to be
used as model colloidal ions, primed for crystallization. '
article_processing_charge: No
article_type: original
author:
- first_name: Theodore
full_name: Hueckel, Theodore
last_name: Hueckel
- first_name: Glen M.
full_name: Hocky, Glen M.
last_name: Hocky
- first_name: Jérémie A
full_name: Palacci, Jérémie A
id: 8fb92548-2b22-11eb-b7c1-a3f0d08d7c7d
last_name: Palacci
orcid: 0000-0002-7253-9465
- first_name: Stefano
full_name: Sacanna, Stefano
last_name: Sacanna
citation:
ama: Hueckel T, Hocky GM, Palacci JA, Sacanna S. Ionic solids from common colloids.
Nature. 2020;580(7804):487-490. doi:10.1038/s41586-020-2205-0
apa: Hueckel, T., Hocky, G. M., Palacci, J. A., & Sacanna, S. (2020). Ionic
solids from common colloids. Nature. Springer Nature. https://doi.org/10.1038/s41586-020-2205-0
chicago: Hueckel, Theodore, Glen M. Hocky, Jérémie A Palacci, and Stefano Sacanna.
“Ionic Solids from Common Colloids.” Nature. Springer Nature, 2020. https://doi.org/10.1038/s41586-020-2205-0.
ieee: T. Hueckel, G. M. Hocky, J. A. Palacci, and S. Sacanna, “Ionic solids from
common colloids,” Nature, vol. 580, no. 7804. Springer Nature, pp. 487–490,
2020.
ista: Hueckel T, Hocky GM, Palacci JA, Sacanna S. 2020. Ionic solids from common
colloids. Nature. 580(7804), 487–490.
mla: Hueckel, Theodore, et al. “Ionic Solids from Common Colloids.” Nature,
vol. 580, no. 7804, Springer Nature, 2020, pp. 487–90, doi:10.1038/s41586-020-2205-0.
short: T. Hueckel, G.M. Hocky, J.A. Palacci, S. Sacanna, Nature 580 (2020) 487–490.
date_created: 2021-02-02T13:30:50Z
date_published: 2020-04-23T00:00:00Z
date_updated: 2023-02-23T13:47:55Z
day: '23'
doi: 10.1038/s41586-020-2205-0
extern: '1'
external_id:
pmid:
- '32322078'
intvolume: ' 580'
issue: '7804'
keyword:
- Multidisciplinary
language:
- iso: eng
month: '04'
oa_version: None
page: 487-490
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ionic solids from common colloids
type: journal_article
user_id: D865714E-FA4E-11E9-B85B-F5C5E5697425
volume: 580
year: '2020'
...
---
_id: '9685'
abstract:
- lang: eng
text: Hydrogen, the simplest and most abundant element in the Universe, develops
a remarkably complex behaviour upon compression^1. Since Wigner predicted the
dissociation and metallization of solid hydrogen at megabar pressures almost a
century ago^2, several efforts have been made to explain the many unusual properties
of dense hydrogen, including a rich and poorly understood solid polymorphism^1,3-5,
an anomalous melting line6 and the possible transition to a superconducting state^7.
Experiments at such extreme conditions are challenging and often lead to hard-to-interpret
and controversial observations, whereas theoretical investigations are constrained
by the huge computational cost of sufficiently accurate quantum mechanical calculations.
Here we present a theoretical study of the phase diagram of dense hydrogen that
uses machine learning to 'learn' potential-energy surfaces and interatomic forces
from reference calculations and then predict them at low computational cost, overcoming
length- and timescale limitations. We reproduce both the re-entrant melting behaviour
and the polymorphism of the solid phase. Simulations using our machine-learning-based
potentials provide evidence for a continuous molecular-to-atomic transition in
the liquid, with no first-order transition observed above the melting line. This
suggests a smooth transition between insulating and metallic layers in giant gas
planets, and reconciles existing discrepancies between experiments as a manifestation
of supercritical behaviour.
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Bingqing
full_name: Cheng, Bingqing
id: cbe3cda4-d82c-11eb-8dc7-8ff94289fcc9
last_name: Cheng
orcid: 0000-0002-3584-9632
- first_name: Guglielmo
full_name: Mazzola, Guglielmo
last_name: Mazzola
- first_name: Chris J.
full_name: Pickard, Chris J.
last_name: Pickard
- first_name: Michele
full_name: Ceriotti, Michele
last_name: Ceriotti
citation:
ama: Cheng B, Mazzola G, Pickard CJ, Ceriotti M. Evidence for supercritical behaviour
of high-pressure liquid hydrogen. Nature. 2020;585(7824):217-220. doi:10.1038/s41586-020-2677-y
apa: Cheng, B., Mazzola, G., Pickard, C. J., & Ceriotti, M. (2020). Evidence
for supercritical behaviour of high-pressure liquid hydrogen. Nature. Springer
Nature. https://doi.org/10.1038/s41586-020-2677-y
chicago: Cheng, Bingqing, Guglielmo Mazzola, Chris J. Pickard, and Michele Ceriotti.
“Evidence for Supercritical Behaviour of High-Pressure Liquid Hydrogen.” Nature.
Springer Nature, 2020. https://doi.org/10.1038/s41586-020-2677-y.
ieee: B. Cheng, G. Mazzola, C. J. Pickard, and M. Ceriotti, “Evidence for supercritical
behaviour of high-pressure liquid hydrogen,” Nature, vol. 585, no. 7824.
Springer Nature, pp. 217–220, 2020.
ista: Cheng B, Mazzola G, Pickard CJ, Ceriotti M. 2020. Evidence for supercritical
behaviour of high-pressure liquid hydrogen. Nature. 585(7824), 217–220.
mla: Cheng, Bingqing, et al. “Evidence for Supercritical Behaviour of High-Pressure
Liquid Hydrogen.” Nature, vol. 585, no. 7824, Springer Nature, 2020, pp.
217–20, doi:10.1038/s41586-020-2677-y.
short: B. Cheng, G. Mazzola, C.J. Pickard, M. Ceriotti, Nature 585 (2020) 217–220.
date_created: 2021-07-19T09:17:49Z
date_published: 2020-09-10T00:00:00Z
date_updated: 2021-08-09T12:38:01Z
day: '10'
doi: 10.1038/s41586-020-2677-y
extern: '1'
external_id:
arxiv:
- '1906.03341'
pmid:
- '32908269'
intvolume: ' 585'
issue: '7824'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/1906.03341
month: '09'
oa: 1
oa_version: Preprint
page: 217-220
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Evidence for supercritical behaviour of high-pressure liquid hydrogen
type: journal_article
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
volume: 585
year: '2020'
...
---
_id: '10618'
abstract:
- lang: eng
text: Magnetism typically arises from the joint effect of Fermi statistics and repulsive
Coulomb interactions, which favours ground states with non-zero electron spin.
As a result, controlling spin magnetism with electric fields—a longstanding technological
goal in spintronics and multiferroics1,2—can be achieved only indirectly. Here
we experimentally demonstrate direct electric-field control of magnetic states
in an orbital Chern insulator3,4,5,6, a magnetic system in which non-trivial band
topology favours long-range order of orbital angular momentum but the spins are
thought to remain disordered7,8,9,10,11,12,13,14. We use van der Waals heterostructures
consisting of a graphene monolayer rotationally faulted with respect to a Bernal-stacked
bilayer to realize narrow and topologically non-trivial valley-projected moiré
minibands15,16,17. At fillings of one and three electrons per moiré unit cell
within these bands, we observe quantized anomalous Hall effects18 with transverse
resistance approximately equal to h/2e2 (where h is Planck’s constant and e is
the charge on the electron), which is indicative of spontaneous polarization of
the system into a single-valley-projected band with a Chern number equal to two.
At a filling of three electrons per moiré unit cell, we find that the sign of
the quantum anomalous Hall effect can be reversed via field-effect control of
the chemical potential; moreover, this transition is hysteretic, which we use
to demonstrate non-volatile electric-field-induced reversal of the magnetic state.
A theoretical analysis19 indicates that the effect arises from the topological
edge states, which drive a change in sign of the magnetization and thus a reversal
in the favoured magnetic state. Voltage control of magnetic states can be used
to electrically pattern non-volatile magnetic-domain structures hosting chiral
edge states, with applications ranging from reconfigurable microwave circuit elements
to ultralow-power magnetic memories.
acknowledgement: We acknowledge discussions with J. Checkelsky, S. Chen, C. Dean,
M. Yankowitz, D. Reilly, I. Sodemann and M. Zaletel. Work at UCSB was primarily
supported by the ARO under MURI W911NF-16-1-0361. Measurements of twisted bilayer
graphene (Extended Data Fig. 8) and measurements at elevated temperatures (Extended
Data Fig. 3) were supported by a SEED grant and made use of shared facilities of
the UCSB MRSEC (NSF DMR 1720256), a member of the Materials Research Facilities
Network (www.mrfn.org). A.F.Y. acknowledges the support of the David and Lucille
Packard Foundation under award 2016-65145. A.H.M. and J.Z. were supported by the
National Science Foundation through the Center for Dynamics and Control of Materials,
an NSF MRSEC under Cooperative Agreement number DMR-1720595, and by the Welch Foundation
under grant TBF1473. C.L.T. acknowledges support from the Hertz Foundation and from
the National Science Foundation Graduate Research Fellowship Program under grant
1650114. K.W. and T.T. acknowledge support from the Elemental Strategy Initiative
conducted by the MEXT, Japan, Grant Number JPMXP0112101001, JSPS KAKENHI grant numbers
JP20H00354 and the CREST(JPMJCR15F3), JST.
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Hryhoriy
full_name: Polshyn, Hryhoriy
id: edfc7cb1-526e-11ec-b05a-e6ecc27e4e48
last_name: Polshyn
orcid: 0000-0001-8223-8896
- first_name: J.
full_name: Zhu, J.
last_name: Zhu
- first_name: M. A.
full_name: Kumar, M. A.
last_name: Kumar
- first_name: Y.
full_name: Zhang, Y.
last_name: Zhang
- first_name: F.
full_name: Yang, F.
last_name: Yang
- first_name: C. L.
full_name: Tschirhart, C. L.
last_name: Tschirhart
- first_name: M.
full_name: Serlin, M.
last_name: Serlin
- first_name: K.
full_name: Watanabe, K.
last_name: Watanabe
- first_name: T.
full_name: Taniguchi, T.
last_name: Taniguchi
- first_name: A. H.
full_name: MacDonald, A. H.
last_name: MacDonald
- first_name: A. F.
full_name: Young, A. F.
last_name: Young
citation:
ama: Polshyn H, Zhu J, Kumar MA, et al. Electrical switching of magnetic order in
an orbital Chern insulator. Nature. 2020;588(7836):66-70. doi:10.1038/s41586-020-2963-8
apa: Polshyn, H., Zhu, J., Kumar, M. A., Zhang, Y., Yang, F., Tschirhart, C. L.,
… Young, A. F. (2020). Electrical switching of magnetic order in an orbital Chern
insulator. Nature. Springer Nature. https://doi.org/10.1038/s41586-020-2963-8
chicago: Polshyn, Hryhoriy, J. Zhu, M. A. Kumar, Y. Zhang, F. Yang, C. L. Tschirhart,
M. Serlin, et al. “Electrical Switching of Magnetic Order in an Orbital Chern
Insulator.” Nature. Springer Nature, 2020. https://doi.org/10.1038/s41586-020-2963-8.
ieee: H. Polshyn et al., “Electrical switching of magnetic order in an orbital
Chern insulator,” Nature, vol. 588, no. 7836. Springer Nature, pp. 66–70,
2020.
ista: Polshyn H, Zhu J, Kumar MA, Zhang Y, Yang F, Tschirhart CL, Serlin M, Watanabe
K, Taniguchi T, MacDonald AH, Young AF. 2020. Electrical switching of magnetic
order in an orbital Chern insulator. Nature. 588(7836), 66–70.
mla: Polshyn, Hryhoriy, et al. “Electrical Switching of Magnetic Order in an Orbital
Chern Insulator.” Nature, vol. 588, no. 7836, Springer Nature, 2020, pp.
66–70, doi:10.1038/s41586-020-2963-8.
short: H. Polshyn, J. Zhu, M.A. Kumar, Y. Zhang, F. Yang, C.L. Tschirhart, M. Serlin,
K. Watanabe, T. Taniguchi, A.H. MacDonald, A.F. Young, Nature 588 (2020) 66–70.
date_created: 2022-01-13T14:12:17Z
date_published: 2020-11-23T00:00:00Z
date_updated: 2022-01-13T14:21:04Z
day: '23'
doi: 10.1038/s41586-020-2963-8
extern: '1'
external_id:
arxiv:
- '2004.11353'
pmid:
- '33230333'
intvolume: ' 588'
issue: '7836'
keyword:
- multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/2004.11353
month: '11'
oa: 1
oa_version: Preprint
page: 66-70
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Electrical switching of magnetic order in an orbital Chern insulator
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 588
year: '2020'
...
---
_id: '12599'
abstract:
- lang: eng
text: Mountains are the water towers of the world, supplying a substantial part
of both natural and anthropogenic water demands1,2. They are highly sensitive
and prone to climate change3,4, yet their importance and vulnerability have not
been quantified at the global scale. Here we present a global water tower index
(WTI), which ranks all water towers in terms of their water-supplying role and
the downstream dependence of ecosystems and society. For each water tower, we
assess its vulnerability related to water stress, governance, hydropolitical tension
and future climatic and socio-economic changes. We conclude that the most important
(highest WTI) water towers are also among the most vulnerable, and that climatic
and socio-economic changes will affect them profoundly. This could negatively
impact 1.9 billion people living in (0.3 billion) or directly downstream of (1.6
billion) mountainous areas. Immediate action is required to safeguard the future
of the world’s most important and vulnerable water towers.
article_processing_charge: No
article_type: original
author:
- first_name: W. W.
full_name: Immerzeel, W. W.
last_name: Immerzeel
- first_name: A. F.
full_name: Lutz, A. F.
last_name: Lutz
- first_name: M.
full_name: Andrade, M.
last_name: Andrade
- first_name: A.
full_name: Bahl, A.
last_name: Bahl
- first_name: H.
full_name: Biemans, H.
last_name: Biemans
- first_name: T.
full_name: Bolch, T.
last_name: Bolch
- first_name: S.
full_name: Hyde, S.
last_name: Hyde
- first_name: S.
full_name: Brumby, S.
last_name: Brumby
- first_name: B. J.
full_name: Davies, B. J.
last_name: Davies
- first_name: A. C.
full_name: Elmore, A. C.
last_name: Elmore
- first_name: A.
full_name: Emmer, A.
last_name: Emmer
- first_name: M.
full_name: Feng, M.
last_name: Feng
- first_name: A.
full_name: Fernández, A.
last_name: Fernández
- first_name: U.
full_name: Haritashya, U.
last_name: Haritashya
- first_name: J. S.
full_name: Kargel, J. S.
last_name: Kargel
- first_name: M.
full_name: Koppes, M.
last_name: Koppes
- first_name: P. D. A.
full_name: Kraaijenbrink, P. D. A.
last_name: Kraaijenbrink
- first_name: A. V.
full_name: Kulkarni, A. V.
last_name: Kulkarni
- first_name: P. A.
full_name: Mayewski, P. A.
last_name: Mayewski
- first_name: S.
full_name: Nepal, S.
last_name: Nepal
- first_name: P.
full_name: Pacheco, P.
last_name: Pacheco
- first_name: T. H.
full_name: Painter, T. H.
last_name: Painter
- first_name: Francesca
full_name: Pellicciotti, Francesca
id: b28f055a-81ea-11ed-b70c-a9fe7f7b0e70
last_name: Pellicciotti
- first_name: H.
full_name: Rajaram, H.
last_name: Rajaram
- first_name: S.
full_name: Rupper, S.
last_name: Rupper
- first_name: A.
full_name: Sinisalo, A.
last_name: Sinisalo
- first_name: A. B.
full_name: Shrestha, A. B.
last_name: Shrestha
- first_name: D.
full_name: Viviroli, D.
last_name: Viviroli
- first_name: Y.
full_name: Wada, Y.
last_name: Wada
- first_name: C.
full_name: Xiao, C.
last_name: Xiao
- first_name: T.
full_name: Yao, T.
last_name: Yao
- first_name: J. E. M.
full_name: Baillie, J. E. M.
last_name: Baillie
citation:
ama: Immerzeel WW, Lutz AF, Andrade M, et al. Importance and vulnerability of the
world’s water towers. Nature. 2020;577(7790):364-369. doi:10.1038/s41586-019-1822-y
apa: Immerzeel, W. W., Lutz, A. F., Andrade, M., Bahl, A., Biemans, H., Bolch, T.,
… Baillie, J. E. M. (2020). Importance and vulnerability of the world’s water
towers. Nature. Springer Nature. https://doi.org/10.1038/s41586-019-1822-y
chicago: Immerzeel, W. W., A. F. Lutz, M. Andrade, A. Bahl, H. Biemans, T. Bolch,
S. Hyde, et al. “Importance and Vulnerability of the World’s Water Towers.” Nature.
Springer Nature, 2020. https://doi.org/10.1038/s41586-019-1822-y.
ieee: W. W. Immerzeel et al., “Importance and vulnerability of the world’s
water towers,” Nature, vol. 577, no. 7790. Springer Nature, pp. 364–369,
2020.
ista: Immerzeel WW, Lutz AF, Andrade M, Bahl A, Biemans H, Bolch T, Hyde S, Brumby
S, Davies BJ, Elmore AC, Emmer A, Feng M, Fernández A, Haritashya U, Kargel JS,
Koppes M, Kraaijenbrink PDA, Kulkarni AV, Mayewski PA, Nepal S, Pacheco P, Painter
TH, Pellicciotti F, Rajaram H, Rupper S, Sinisalo A, Shrestha AB, Viviroli D,
Wada Y, Xiao C, Yao T, Baillie JEM. 2020. Importance and vulnerability of the
world’s water towers. Nature. 577(7790), 364–369.
mla: Immerzeel, W. W., et al. “Importance and Vulnerability of the World’s Water
Towers.” Nature, vol. 577, no. 7790, Springer Nature, 2020, pp. 364–69,
doi:10.1038/s41586-019-1822-y.
short: W.W. Immerzeel, A.F. Lutz, M. Andrade, A. Bahl, H. Biemans, T. Bolch, S.
Hyde, S. Brumby, B.J. Davies, A.C. Elmore, A. Emmer, M. Feng, A. Fernández, U.
Haritashya, J.S. Kargel, M. Koppes, P.D.A. Kraaijenbrink, A.V. Kulkarni, P.A.
Mayewski, S. Nepal, P. Pacheco, T.H. Painter, F. Pellicciotti, H. Rajaram, S.
Rupper, A. Sinisalo, A.B. Shrestha, D. Viviroli, Y. Wada, C. Xiao, T. Yao, J.E.M.
Baillie, Nature 577 (2020) 364–369.
date_created: 2023-02-20T08:12:53Z
date_published: 2020-01-16T00:00:00Z
date_updated: 2023-02-28T12:17:38Z
day: '16'
doi: 10.1038/s41586-019-1822-y
extern: '1'
intvolume: ' 577'
issue: '7790'
language:
- iso: eng
month: '01'
oa_version: None
page: 364-369
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Importance and vulnerability of the world’s water towers
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 577
year: '2020'
...