[{"doi":"10.1038/s41586-023-06399-5","abstract":[{"text":"Flows through pipes and channels are, in practice, almost always turbulent, and the multiscale eddying motion is responsible for a major part of the encountered friction losses and pumping costs1. Conversely, for pulsatile flows, in particular for aortic blood flow, turbulence levels remain low despite relatively large peak velocities. For aortic blood flow, high turbulence levels are intolerable as they would damage the shear-sensitive endothelial cell layer2,3,4,5. Here we show that turbulence in ordinary pipe flow is diminished if the flow is driven in a pulsatile mode that incorporates all the key features of the cardiac waveform. At Reynolds numbers comparable to those of aortic blood flow, turbulence is largely inhibited, whereas at much higher speeds, the turbulent drag is reduced by more than 25%. This specific operation mode is more efficient when compared with steady driving, which is the present situation for virtually all fluid transport processes ranging from heating circuits to water, gas and oil pipelines.","lang":"eng"}],"issue":"7977","_id":"14341","title":"Turbulence suppression by cardiac-cycle-inspired driving of pipe flow","author":[{"orcid":"0000-0001-5227-4271","id":"40315C30-F248-11E8-B48F-1D18A9856A87","first_name":"Davide","full_name":"Scarselli, Davide","last_name":"Scarselli"},{"first_name":"Jose M","id":"40770848-F248-11E8-B48F-1D18A9856A87","last_name":"Lopez Alonso","full_name":"Lopez Alonso, Jose M","orcid":"0000-0002-0384-2022"},{"orcid":"0000-0002-3072-5999","first_name":"Atul","id":"2A2006B2-F248-11E8-B48F-1D18A9856A87","last_name":"Varshney","full_name":"Varshney, Atul"},{"full_name":"Hof, Björn","last_name":"Hof","id":"3A374330-F248-11E8-B48F-1D18A9856A87","first_name":"Björn","orcid":"0000-0003-2057-2754"}],"publication_status":"published","acknowledgement":"We acknowledge the assistance of the Miba machine shop and the team of the ISTA-HPC cluster. We thank M. Quadrio for the discussions. The work was supported by the Simons Foundation (grant no. 662960) and by the Austrian Science Fund (grant no. I4188-N30), within Deutsche Forschungsgemeinschaft research unit FOR 2688.","status":"public","project":[{"name":"Revisiting the Turbulence Problem Using Statistical Mechanics: Experimental Studies on Transitional and Turbulent Flows","_id":"238598C6-32DE-11EA-91FC-C7463DDC885E","grant_number":"662960"},{"name":"Instabilities in pulsating pipe flow of Newtonian and complex fluids","call_identifier":"FWF","grant_number":"I04188","_id":"238B8092-32DE-11EA-91FC-C7463DDC885E"}],"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"oa_version":"None","pmid":1,"month":"09","citation":{"chicago":"Scarselli, Davide, Jose M Lopez Alonso, Atul Varshney, and Björn Hof. “Turbulence Suppression by Cardiac-Cycle-Inspired Driving of Pipe Flow.” <i>Nature</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41586-023-06399-5\">https://doi.org/10.1038/s41586-023-06399-5</a>.","mla":"Scarselli, Davide, et al. “Turbulence Suppression by Cardiac-Cycle-Inspired Driving of Pipe Flow.” <i>Nature</i>, vol. 621, no. 7977, Springer Nature, 2023, pp. 71–74, doi:<a href=\"https://doi.org/10.1038/s41586-023-06399-5\">10.1038/s41586-023-06399-5</a>.","apa":"Scarselli, D., Lopez Alonso, J. M., Varshney, A., &#38; Hof, B. (2023). Turbulence suppression by cardiac-cycle-inspired driving of pipe flow. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-023-06399-5\">https://doi.org/10.1038/s41586-023-06399-5</a>","ieee":"D. Scarselli, J. M. Lopez Alonso, A. Varshney, and B. Hof, “Turbulence suppression by cardiac-cycle-inspired driving of pipe flow,” <i>Nature</i>, vol. 621, no. 7977. Springer Nature, pp. 71–74, 2023.","ista":"Scarselli D, Lopez Alonso JM, Varshney A, Hof B. 2023. Turbulence suppression by cardiac-cycle-inspired driving of pipe flow. Nature. 621(7977), 71–74.","short":"D. Scarselli, J.M. Lopez Alonso, A. Varshney, B. Hof, Nature 621 (2023) 71–74.","ama":"Scarselli D, Lopez Alonso JM, Varshney A, Hof B. Turbulence suppression by cardiac-cycle-inspired driving of pipe flow. <i>Nature</i>. 2023;621(7977):71-74. doi:<a href=\"https://doi.org/10.1038/s41586-023-06399-5\">10.1038/s41586-023-06399-5</a>"},"day":"07","volume":621,"article_processing_charge":"No","year":"2023","page":"71-74","article_type":"original","related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://www.ista.ac.at/en/news/pumping-like-the-heart/"}]},"date_updated":"2023-09-20T12:10:22Z","publication":"Nature","date_created":"2023-09-17T22:01:09Z","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"ScienComp"}],"scopus_import":"1","date_published":"2023-09-07T00:00:00Z","external_id":{"pmid":["37673988"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"BjHo"}],"quality_controlled":"1","type":"journal_article","language":[{"iso":"eng"}],"publisher":"Springer Nature","intvolume":"       621"},{"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"publication_status":"epub_ahead","acknowledgement":"We thank the Human Embryonic Stem Cell Unit, Advanced Light Microscopy and High-throughput Screening facilities at the Crick for their support in various aspects of the work. We thank the laboratory of P. Anderson for providing the G3BP-DKO U2OS cells. The authors thank N. Chen for providing the purified glycinin protein; Z. Zhao for providing the microfluidic chip wafers; and M. Amaral and F. Frey for helpful discussions and valuable input regarding analysis methods. This work was supported by the Francis Crick Institute (to M.G.G.), which receives its core funding from Cancer Research UK (FC001092), the UK Medical Research Council (FC001092) and the Wellcome Trust (FC001092). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 772022 to M.G.G.). C.B. has received funding from the European Respiratory Society and the European Union’s H2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement no. 713406. A.M. acknowledges support from Alexander von Humboldt Foundation and C.V.-C. acknowledges funding by the Royal Society and the European Research Council under the European Union’s Horizon 2020 Research and Innovation Programme (grant no. 802960 to A.S.). All simulations were carried out on the high-performance computing cluster at the Institute of Science and Technology Austria. For the purpose of Open Access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.\r\nOpen Access funding provided by The Francis Crick Institute.","status":"public","_id":"14610","title":"Stress granules plug and stabilize damaged endolysosomal membranes","author":[{"first_name":"Claudio","full_name":"Bussi, Claudio","last_name":"Bussi"},{"first_name":"Agustín","last_name":"Mangiarotti","full_name":"Mangiarotti, Agustín"},{"first_name":"Christian Eduardo","id":"3adeca52-9313-11ed-b1ac-c170b2505714","last_name":"Vanhille-Campos","full_name":"Vanhille-Campos, Christian Eduardo"},{"first_name":"Beren","full_name":"Aylan, Beren","last_name":"Aylan"},{"last_name":"Pellegrino","full_name":"Pellegrino, Enrica","first_name":"Enrica"},{"first_name":"Natalia","full_name":"Athanasiadi, Natalia","last_name":"Athanasiadi"},{"first_name":"Antony","full_name":"Fearns, Antony","last_name":"Fearns"},{"full_name":"Rodgers, Angela","last_name":"Rodgers","first_name":"Angela"},{"last_name":"Franzmann","full_name":"Franzmann, Titus M.","first_name":"Titus M."},{"orcid":"0000-0002-7854-2139","last_name":"Šarić","full_name":"Šarić, Anđela","first_name":"Anđela","id":"bf63d406-f056-11eb-b41d-f263a6566d8b"},{"last_name":"Dimova","full_name":"Dimova, Rumiana","first_name":"Rumiana"},{"last_name":"Gutierrez","full_name":"Gutierrez, Maximiliano G.","first_name":"Maximiliano G."}],"doi":"10.1038/s41586-023-06726-w","abstract":[{"lang":"eng","text":"<jats:title>Abstract</jats:title><jats:p>Endomembrane damage represents a form of stress that is detrimental for eukaryotic cells<jats:sup>1,2</jats:sup>. To cope with this threat, cells possess mechanisms that repair the damage and restore cellular homeostasis<jats:sup>3–7</jats:sup>. Endomembrane damage also results in organelle instability and the mechanisms by which cells stabilize damaged endomembranes to enable membrane repair remains unknown. Here, by combining in vitro and in cellulo studies with computational modelling we uncover a biological function for stress granules whereby these biomolecular condensates form rapidly at endomembrane damage sites and act as a plug that stabilizes the ruptured membrane. Functionally, we demonstrate that stress granule formation and membrane stabilization enable efficient repair of damaged endolysosomes, through both ESCRT (endosomal sorting complex required for transport)-dependent and independent mechanisms. We also show that blocking stress granule formation in human macrophages creates a permissive environment for <jats:italic>Mycobacterium tuberculosis</jats:italic>, a human pathogen that exploits endomembrane damage to survive within the host.</jats:p>"}],"article_processing_charge":"Yes (via OA deal)","month":"11","pmid":1,"citation":{"apa":"Bussi, C., Mangiarotti, A., Vanhille-Campos, C. E., Aylan, B., Pellegrino, E., Athanasiadi, N., … Gutierrez, M. G. (2023). Stress granules plug and stabilize damaged endolysosomal membranes. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-023-06726-w\">https://doi.org/10.1038/s41586-023-06726-w</a>","ieee":"C. Bussi <i>et al.</i>, “Stress granules plug and stabilize damaged endolysosomal membranes,” <i>Nature</i>. Springer Nature, 2023.","ama":"Bussi C, Mangiarotti A, Vanhille-Campos CE, et al. Stress granules plug and stabilize damaged endolysosomal membranes. <i>Nature</i>. 2023. doi:<a href=\"https://doi.org/10.1038/s41586-023-06726-w\">10.1038/s41586-023-06726-w</a>","short":"C. Bussi, A. Mangiarotti, C.E. Vanhille-Campos, B. Aylan, E. Pellegrino, N. Athanasiadi, A. Fearns, A. Rodgers, T.M. Franzmann, A. Šarić, R. Dimova, M.G. Gutierrez, Nature (2023).","ista":"Bussi C, Mangiarotti A, Vanhille-Campos CE, Aylan B, Pellegrino E, Athanasiadi N, Fearns A, Rodgers A, Franzmann TM, Šarić A, Dimova R, Gutierrez MG. 2023. Stress granules plug and stabilize damaged endolysosomal membranes. Nature.","chicago":"Bussi, Claudio, Agustín Mangiarotti, Christian Eduardo Vanhille-Campos, Beren Aylan, Enrica Pellegrino, Natalia Athanasiadi, Antony Fearns, et al. “Stress Granules Plug and Stabilize Damaged Endolysosomal Membranes.” <i>Nature</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41586-023-06726-w\">https://doi.org/10.1038/s41586-023-06726-w</a>.","mla":"Bussi, Claudio, et al. “Stress Granules Plug and Stabilize Damaged Endolysosomal Membranes.” <i>Nature</i>, Springer Nature, 2023, doi:<a href=\"https://doi.org/10.1038/s41586-023-06726-w\">10.1038/s41586-023-06726-w</a>."},"day":"15","oa_version":"Published Version","date_updated":"2023-11-27T09:05:08Z","publication":"Nature","article_type":"original","related_material":{"link":[{"url":"https://doi.org/10.1038/s41586-023-06882-z","relation":"erratum"}],"record":[{"status":"public","relation":"research_data","id":"14472"}]},"oa":1,"keyword":["Multidisciplinary"],"year":"2023","publisher":"Springer Nature","main_file_link":[{"url":"https://doi.org/10.1038/s41586-023-06726-w","open_access":"1"}],"type":"journal_article","language":[{"iso":"eng"}],"quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"AnSa"}],"date_created":"2023-11-27T07:56:37Z","external_id":{"pmid":["37968398"]},"date_published":"2023-11-15T00:00:00Z"},{"ddc":["570"],"month":"06","file_date_updated":"2023-11-14T11:48:18Z","day":"29","citation":{"mla":"Degen, Morris, et al. “Structural Basis of NINJ1-Mediated Plasma Membrane Rupture in Cell Death.” <i>Nature</i>, vol. 618, Springer Nature, 2023, pp. 1065–71, doi:<a href=\"https://doi.org/10.1038/s41586-023-05991-z\">10.1038/s41586-023-05991-z</a>.","chicago":"Degen, Morris, José Carlos Santos, Kristyna Pluhackova, Gonzalo Cebrero, Saray Ramos, Gytis Jankevicius, Ella Hartenian, et al. “Structural Basis of NINJ1-Mediated Plasma Membrane Rupture in Cell Death.” <i>Nature</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41586-023-05991-z\">https://doi.org/10.1038/s41586-023-05991-z</a>.","short":"M. Degen, J.C. Santos, K. Pluhackova, G. Cebrero, S. Ramos, G. Jankevicius, E. Hartenian, U. Guillerm, S.A. Mari, B. Kohl, D.J. Müller, P. Schanda, T. Maier, C. Perez, C. Sieben, P. Broz, S. Hiller, Nature 618 (2023) 1065–1071.","ista":"Degen M, Santos JC, Pluhackova K, Cebrero G, Ramos S, Jankevicius G, Hartenian E, Guillerm U, Mari SA, Kohl B, Müller DJ, Schanda P, Maier T, Perez C, Sieben C, Broz P, Hiller S. 2023. Structural basis of NINJ1-mediated plasma membrane rupture in cell death. Nature. 618, 1065–1071.","ama":"Degen M, Santos JC, Pluhackova K, et al. Structural basis of NINJ1-mediated plasma membrane rupture in cell death. <i>Nature</i>. 2023;618:1065-1071. doi:<a href=\"https://doi.org/10.1038/s41586-023-05991-z\">10.1038/s41586-023-05991-z</a>","apa":"Degen, M., Santos, J. C., Pluhackova, K., Cebrero, G., Ramos, S., Jankevicius, G., … Hiller, S. (2023). Structural basis of NINJ1-mediated plasma membrane rupture in cell death. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-023-05991-z\">https://doi.org/10.1038/s41586-023-05991-z</a>","ieee":"M. Degen <i>et al.</i>, “Structural basis of NINJ1-mediated plasma membrane rupture in cell death,” <i>Nature</i>, vol. 618. Springer Nature, pp. 1065–1071, 2023."},"oa_version":"Published Version","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"file":[{"creator":"dernst","relation":"main_file","file_size":12292188,"file_name":"2023_Nature_Degen.pdf","date_updated":"2023-11-14T11:48:18Z","success":1,"checksum":"0fab69252453bff1de7f0e2eceb76d34","content_type":"application/pdf","access_level":"open_access","date_created":"2023-11-14T11:48:18Z","file_id":"14533"}],"article_processing_charge":"Yes (via OA deal)","volume":618,"has_accepted_license":"1","_id":"13096","author":[{"first_name":"Morris","last_name":"Degen","full_name":"Degen, Morris"},{"first_name":"José Carlos","last_name":"Santos","full_name":"Santos, José Carlos"},{"first_name":"Kristyna","last_name":"Pluhackova","full_name":"Pluhackova, Kristyna"},{"last_name":"Cebrero","full_name":"Cebrero, Gonzalo","first_name":"Gonzalo"},{"full_name":"Ramos, Saray","last_name":"Ramos","first_name":"Saray"},{"first_name":"Gytis","full_name":"Jankevicius, Gytis","last_name":"Jankevicius"},{"first_name":"Ella","full_name":"Hartenian, Ella","last_name":"Hartenian"},{"last_name":"Guillerm","full_name":"Guillerm, Undina","first_name":"Undina","id":"bb74f472-ae54-11eb-9835-bc9c22fb1183"},{"first_name":"Stefania A.","last_name":"Mari","full_name":"Mari, Stefania A."},{"first_name":"Bastian","full_name":"Kohl, Bastian","last_name":"Kohl"},{"first_name":"Daniel J.","full_name":"Müller, Daniel J.","last_name":"Müller"},{"orcid":"0000-0002-9350-7606","first_name":"Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","last_name":"Schanda","full_name":"Schanda, Paul"},{"full_name":"Maier, Timm","last_name":"Maier","first_name":"Timm"},{"first_name":"Camilo","last_name":"Perez","full_name":"Perez, Camilo"},{"first_name":"Christian","last_name":"Sieben","full_name":"Sieben, Christian"},{"first_name":"Petr","full_name":"Broz, Petr","last_name":"Broz"},{"last_name":"Hiller","full_name":"Hiller, Sebastian","first_name":"Sebastian"}],"title":"Structural basis of NINJ1-mediated plasma membrane rupture in cell death","doi":"10.1038/s41586-023-05991-z","abstract":[{"text":"Eukaryotic cells can undergo different forms of programmed cell death, many of which culminate in plasma membrane rupture as the defining terminal event1,2,3,4,5,6,7. Plasma membrane rupture was long thought to be driven by osmotic pressure, but it has recently been shown to be in many cases an active process, mediated by the protein ninjurin-18 (NINJ1). Here we resolve the structure of NINJ1 and the mechanism by which it ruptures membranes. Super-resolution microscopy reveals that NINJ1 clusters into structurally diverse assemblies in the membranes of dying cells, in particular large, filamentous assemblies with branched morphology. A cryo-electron microscopy structure of NINJ1 filaments shows a tightly packed fence-like array of transmembrane α-helices. Filament directionality and stability is defined by two amphipathic α-helices that interlink adjacent filament subunits. The NINJ1 filament features a hydrophilic side and a hydrophobic side, and molecular dynamics simulations show that it can stably cap membrane edges. The function of the resulting supramolecular arrangement was validated by site-directed mutagenesis. Our data thus suggest that, during lytic cell death, the extracellular α-helices of NINJ1 insert into the plasma membrane to polymerize NINJ1 monomers into amphipathic filaments that rupture the plasma membrane. The membrane protein NINJ1 is therefore an interactive component of the eukaryotic cell membrane that functions as an in-built breaking point in response to activation of cell death.","lang":"eng"}],"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"status":"public","publication_status":"published","acknowledgement":"This work was supported by the Deutsche Forschungsgemeinschaft under Germany’s Excellence Strategy EXC 2075–390740016 and the Stuttgart Center for Simulation Science (SC SimTech) to K.P., by ERC-CoG 770988 (InflamCellDeath) and SNF Project funding (310030B_198005, 310030B_192523) to P.B., by the Swiss Nanoscience Institute and the Swiss National Science Foundation via the NCCR AntiResist (180541) to S.H. and the NCCR Molecular Systems Engineering (51NF40-205608) to D.J.M., by the Helmholtz Young Investigator Program of the Helmholtz Association to C.S., by the SNF Professorship funding (PP00P3_198903) to C.P., EMBO postdoctoral fellowship ALTF 27-2022 to E.H. and by the Scientific Service Units of IST Austria through resources provided by the NMR and Life Science Facilities to P.S. Molecular dynamics simulations were performed on the HoreKa supercomputer funded by the Ministry of Science, Research and the Arts Baden-Württemberg and by the Federal Ministry of Education and Research. The authors thank the BioEM Lab of the Biozentrum, University of Basel for support; V. Mack, K. Shkarina and J. Fricke for technical support; D. Ricklin and S. Vogt for peptide synthesis; P. Pelczar for support with animals; S.-J. Marrink and P. Telles de Souza for supply with Martini3 parameters and scripts; and P. Radler und M. Loose for help with QCM. Fig. 4g and Extended Data Fig. 1a were in part created with BioRender.com.\r\nOpen access funding provided by University of Basel.","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"PaSc"}],"acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}],"scopus_import":"1","date_created":"2023-05-28T22:01:04Z","date_published":"2023-06-29T00:00:00Z","external_id":{"isi":["000991386800011"]},"intvolume":"       618","publisher":"Springer Nature","type":"journal_article","language":[{"iso":"eng"}],"quality_controlled":"1","isi":1,"oa":1,"year":"2023","date_updated":"2023-11-14T11:49:21Z","publication":"Nature","article_type":"original","page":"1065-1071"},{"doi":"10.1038/s41586-023-06018-3","abstract":[{"text":"A density wave (DW) is a fundamental type of long-range order in quantum matter tied to self-organization into a crystalline structure. The interplay of DW order with superfluidity can lead to complex scenarios that pose a great challenge to theoretical analysis. In the past decades, tunable quantum Fermi gases have served as model systems for exploring the physics of strongly interacting fermions, including most notably magnetic ordering1, pairing and superfluidity2, and the crossover from a Bardeen–Cooper–Schrieffer superfluid to a Bose–Einstein condensate3. Here, we realize a Fermi gas featuring both strong, tunable contact interactions and photon-mediated, spatially structured long-range interactions in a transversely driven high-finesse optical cavity. Above a critical long-range interaction strength, DW order is stabilized in the system, which we identify via its superradiant light-scattering properties. We quantitatively measure the variation of the onset of DW order as the contact interaction is varied across the Bardeen–Cooper–Schrieffer superfluid and Bose–Einstein condensate crossover, in qualitative agreement with a mean-field theory. The atomic DW susceptibility varies over an order of magnitude upon tuning the strength and the sign of the long-range interactions below the self-ordering threshold, demonstrating independent and simultaneous control over the contact and long-range interactions. Therefore, our experimental setup provides a fully tunable and microscopically controllable platform for the experimental study of the interplay of superfluidity and DW order.","lang":"eng"}],"_id":"13119","author":[{"full_name":"Helson, Victor","last_name":"Helson","first_name":"Victor"},{"first_name":"Timo","last_name":"Zwettler","full_name":"Zwettler, Timo"},{"last_name":"Mivehvar","full_name":"Mivehvar, Farokh","first_name":"Farokh"},{"first_name":"Elvia","full_name":"Colella, Elvia","last_name":"Colella"},{"id":"53f93ea2-803f-11ed-ab7e-b283135794ef","first_name":"Kevin Etienne Robert","full_name":"Roux, Kevin Etienne Robert","last_name":"Roux"},{"last_name":"Konishi","full_name":"Konishi, Hideki","first_name":"Hideki"},{"full_name":"Ritsch, Helmut","last_name":"Ritsch","first_name":"Helmut"},{"full_name":"Brantut, Jean Philippe","last_name":"Brantut","first_name":"Jean Philippe"}],"title":"Density-wave ordering in a unitary Fermi gas with photon-mediated interactions","acknowledgement":"Open access funding provided by EPFL Lausanne.We acknowledge discussions with T. Donner and T. Esslinger. We thank G. del Pace and T. Bühler for their assistance in the final stages of the experiment. We acknowledge funding from the European Research Council under the European Union Horizon 2020 Research and Innovation Programme (Grant no. 714309) and the Swiss National Science Foundation (Grant no. 184654). F.M. acknowledges financial support from the Austrian Science Fund (Stand-Alone Project P 35891-N).","publication_status":"published","status":"public","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"oa_version":"Published Version","file_date_updated":"2023-11-14T13:00:19Z","ddc":["530"],"month":"06","day":"22","citation":{"ista":"Helson V, Zwettler T, Mivehvar F, Colella E, Roux KER, Konishi H, Ritsch H, Brantut JP. 2023. Density-wave ordering in a unitary Fermi gas with photon-mediated interactions. Nature. 618, 716–720.","short":"V. Helson, T. Zwettler, F. Mivehvar, E. Colella, K.E.R. Roux, H. Konishi, H. Ritsch, J.P. Brantut, Nature 618 (2023) 716–720.","ama":"Helson V, Zwettler T, Mivehvar F, et al. Density-wave ordering in a unitary Fermi gas with photon-mediated interactions. <i>Nature</i>. 2023;618:716-720. doi:<a href=\"https://doi.org/10.1038/s41586-023-06018-3\">10.1038/s41586-023-06018-3</a>","apa":"Helson, V., Zwettler, T., Mivehvar, F., Colella, E., Roux, K. E. R., Konishi, H., … Brantut, J. P. (2023). Density-wave ordering in a unitary Fermi gas with photon-mediated interactions. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-023-06018-3\">https://doi.org/10.1038/s41586-023-06018-3</a>","ieee":"V. Helson <i>et al.</i>, “Density-wave ordering in a unitary Fermi gas with photon-mediated interactions,” <i>Nature</i>, vol. 618. Springer Nature, pp. 716–720, 2023.","chicago":"Helson, Victor, Timo Zwettler, Farokh Mivehvar, Elvia Colella, Kevin Etienne Robert Roux, Hideki Konishi, Helmut Ritsch, and Jean Philippe Brantut. “Density-Wave Ordering in a Unitary Fermi Gas with Photon-Mediated Interactions.” <i>Nature</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41586-023-06018-3\">https://doi.org/10.1038/s41586-023-06018-3</a>.","mla":"Helson, Victor, et al. “Density-Wave Ordering in a Unitary Fermi Gas with Photon-Mediated Interactions.” <i>Nature</i>, vol. 618, Springer Nature, 2023, pp. 716–20, doi:<a href=\"https://doi.org/10.1038/s41586-023-06018-3\">10.1038/s41586-023-06018-3</a>."},"volume":618,"article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","file":[{"success":1,"date_updated":"2023-11-14T13:00:19Z","access_level":"open_access","checksum":"4887a296e3b6f54e8c0b946cbfd24f49","content_type":"application/pdf","file_name":"2023_Nature_Helson.pdf","relation":"main_file","creator":"dernst","file_size":8156497,"file_id":"14534","date_created":"2023-11-14T13:00:19Z"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"year":"2023","oa":1,"article_type":"original","page":"716-720","date_updated":"2023-11-14T13:02:50Z","publication":"Nature","scopus_import":"1","date_created":"2023-06-04T22:01:03Z","date_published":"2023-06-22T00:00:00Z","external_id":{"isi":["001001139300008"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"GeKa"}],"language":[{"iso":"eng"}],"quality_controlled":"1","type":"journal_article","isi":1,"intvolume":"       618","publisher":"Springer Nature"},{"file_date_updated":"2022-08-05T06:08:24Z","ddc":["570"],"pmid":1,"month":"05","citation":{"ama":"Lukacisin M, Espinosa-Cantú A, Bollenbach MT. Intron-mediated induction of phenotypic heterogeneity. <i>Nature</i>. 2022;605:113-118. doi:<a href=\"https://doi.org/10.1038/s41586-022-04633-0\">10.1038/s41586-022-04633-0</a>","ista":"Lukacisin M, Espinosa-Cantú A, Bollenbach MT. 2022. Intron-mediated induction of phenotypic heterogeneity. Nature. 605, 113–118.","short":"M. Lukacisin, A. Espinosa-Cantú, M.T. Bollenbach, Nature 605 (2022) 113–118.","apa":"Lukacisin, M., Espinosa-Cantú, A., &#38; Bollenbach, M. T. (2022). Intron-mediated induction of phenotypic heterogeneity. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-04633-0\">https://doi.org/10.1038/s41586-022-04633-0</a>","ieee":"M. Lukacisin, A. Espinosa-Cantú, and M. T. Bollenbach, “Intron-mediated induction of phenotypic heterogeneity,” <i>Nature</i>, vol. 605. Springer Nature, pp. 113–118, 2022.","chicago":"Lukacisin, Martin, Adriana Espinosa-Cantú, and Mark Tobias Bollenbach. “Intron-Mediated Induction of Phenotypic Heterogeneity.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-04633-0\">https://doi.org/10.1038/s41586-022-04633-0</a>.","mla":"Lukacisin, Martin, et al. “Intron-Mediated Induction of Phenotypic Heterogeneity.” <i>Nature</i>, vol. 605, Springer Nature, 2022, pp. 113–18, doi:<a href=\"https://doi.org/10.1038/s41586-022-04633-0\">10.1038/s41586-022-04633-0</a>."},"day":"05","oa_version":"Published Version","file":[{"file_name":"2022_Nature_Lukacisin.pdf","file_size":25360311,"creator":"dernst","relation":"main_file","access_level":"open_access","checksum":"d68cd1596bb9fd819b750fe47c8a138a","content_type":"application/pdf","success":1,"date_updated":"2022-08-05T06:08:24Z","file_id":"11727","date_created":"2022-08-05T06:08:24Z"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"article_processing_charge":"No","volume":605,"has_accepted_license":"1","_id":"11341","title":"Intron-mediated induction of phenotypic heterogeneity","author":[{"last_name":"Lukacisin","full_name":"Lukacisin, Martin","first_name":"Martin","id":"298FFE8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6549-4177"},{"full_name":"Espinosa-Cantú, Adriana","last_name":"Espinosa-Cantú","first_name":"Adriana"},{"first_name":"Mark Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","last_name":"Bollenbach","full_name":"Bollenbach, Mark Tobias","orcid":"0000-0003-4398-476X"}],"doi":"10.1038/s41586-022-04633-0","abstract":[{"lang":"eng","text":"Intragenic regions that are removed during maturation of the RNA transcript—introns—are universally present in the nuclear genomes of eukaryotes1. The budding yeast, an otherwise intron-poor species, preserves two sets of ribosomal protein genes that differ primarily in their introns2,3. Although studies have shed light on the role of ribosomal protein introns under stress and starvation4,5,6, understanding the contribution of introns to ribosome regulation remains challenging. Here, by combining isogrowth profiling7 with single-cell protein measurements8, we show that introns can mediate inducible phenotypic heterogeneity that confers a clear fitness advantage. Osmotic stress leads to bimodal expression of the small ribosomal subunit protein Rps22B, which is mediated by an intron in the 5′ untranslated region of its transcript. The two resulting yeast subpopulations differ in their ability to cope with starvation. Low levels of Rps22B protein result in prolonged survival under sustained starvation, whereas high levels of Rps22B enable cells to grow faster after transient starvation. Furthermore, yeasts growing at high concentrations of sugar, similar to those in ripe grapes, exhibit bimodal expression of Rps22B when approaching the stationary phase. Differential intron-mediated regulation of ribosomal protein genes thus provides a way to diversify the population when starvation threatens in natural environments. Our findings reveal a role for introns in inducing phenotypic heterogeneity in changing environments, and suggest that duplicated ribosomal protein genes in yeast contribute to resolving the evolutionary conflict between precise expression control and environmental responsiveness9."}],"project":[{"name":"Optimality principles in responses to antibiotics","call_identifier":"FP7","_id":"25E83C2C-B435-11E9-9278-68D0E5697425","grant_number":"303507"},{"call_identifier":"FWF","name":"Revealing the mechanisms underlying drug interactions","_id":"25E9AF9E-B435-11E9-9278-68D0E5697425","grant_number":"P27201-B22"}],"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"ec_funded":1,"publication_status":"published","acknowledgement":"We thank the IST Austria Life Science Facility, the Miba Machine Shop and M. Lukačišinová for support with the liquid handling robot; the Bioimaging Facility at IST Austria, J. Power and B. Meier at the University of Cologne, and C. Göttlinger at the FACS Analysis Facility at the Institute for Genetics, University of Cologne, for support with flow cytometry experiments; L. Horst for the development of the automated experimental methods in Cologne; J. Parenteau, S. Abou Elela, G. Stormo, M. Springer and M. Schuldiner for providing us with yeast strains; B. Fernando, T. Fink, G. Ansmann and G. Chevreau for technical support; H. Köver, G. Tkačik, N. Barton, A. Angermayr and B. Kavčič for support during laboratory relocation; D. Siekhaus, M. Springer and all the members of the Bollenbach group for support and discussions; and K. Mitosch, M. Lukačišinová, G. Liti and A. de Luna for critical reading of our manuscript. This work was supported in part by an Austrian Science Fund (FWF) standalone grant P 27201-B22 (to T.B.), HFSP program Grant RGP0042/2013 (to T.B.), EU Marie Curie Career Integration Grant No. 303507, and German Research Foundation (DFG) Collaborative Research Centre (SFB) 1310 (to T.B.). A.E.-C. was supported by a Georg Forster fellowship from the Alexander von Humboldt Foundation.","status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_created":"2022-05-01T22:01:42Z","scopus_import":"1","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"Bio"}],"external_id":{"isi":["000784934100003"],"pmid":["35444278"]},"date_published":"2022-05-05T00:00:00Z","publisher":"Springer Nature","intvolume":"       605","quality_controlled":"1","language":[{"iso":"eng"}],"type":"journal_article","isi":1,"oa":1,"year":"2022","date_updated":"2023-08-03T06:44:50Z","publication":"Nature","page":"113-118","article_type":"original"},{"year":"2022","keyword":["Multidisciplinary"],"publication":"Nature","date_updated":"2023-10-18T06:26:30Z","page":"638-639","article_type":"letter_note","department":[{"_id":"MaIb"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2022-12-21T00:00:00Z","external_id":{"pmid":["36543947"]},"date_created":"2023-10-17T11:14:43Z","publisher":"Springer Nature","intvolume":"       612","quality_controlled":"1","type":"journal_article","language":[{"iso":"eng"}],"title":"Molecular engineering enables bright blue LEDs","author":[{"first_name":"Hendrik","last_name":"Utzat","full_name":"Utzat, Hendrik"},{"orcid":"0000-0001-5013-2843","id":"43C61214-F248-11E8-B48F-1D18A9856A87","first_name":"Maria","full_name":"Ibáñez, Maria","last_name":"Ibáñez"}],"_id":"14437","abstract":[{"lang":"eng","text":"Future LEDs could be based on lead halide perovskites. A breakthrough in preparing device-compatible solids composed of nanoscale perovskite crystals overcomes a long-standing hurdle in making blue perovskite LEDs."}],"issue":"7941","doi":"10.1038/d41586-022-04447-0","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"publication_status":"published","status":"public","citation":{"chicago":"Utzat, Hendrik, and Maria Ibáñez. “Molecular Engineering Enables Bright Blue LEDs.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/d41586-022-04447-0\">https://doi.org/10.1038/d41586-022-04447-0</a>.","mla":"Utzat, Hendrik, and Maria Ibáñez. “Molecular Engineering Enables Bright Blue LEDs.” <i>Nature</i>, vol. 612, no. 7941, Springer Nature, 2022, pp. 638–39, doi:<a href=\"https://doi.org/10.1038/d41586-022-04447-0\">10.1038/d41586-022-04447-0</a>.","ista":"Utzat H, Ibáñez M. 2022. Molecular engineering enables bright blue LEDs. Nature. 612(7941), 638–639.","short":"H. Utzat, M. Ibáñez, Nature 612 (2022) 638–639.","ama":"Utzat H, Ibáñez M. Molecular engineering enables bright blue LEDs. <i>Nature</i>. 2022;612(7941):638-639. doi:<a href=\"https://doi.org/10.1038/d41586-022-04447-0\">10.1038/d41586-022-04447-0</a>","apa":"Utzat, H., &#38; Ibáñez, M. (2022). Molecular engineering enables bright blue LEDs. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/d41586-022-04447-0\">https://doi.org/10.1038/d41586-022-04447-0</a>","ieee":"H. Utzat and M. Ibáñez, “Molecular engineering enables bright blue LEDs,” <i>Nature</i>, vol. 612, no. 7941. Springer Nature, pp. 638–639, 2022."},"day":"21","pmid":1,"month":"12","oa_version":"None","volume":612,"article_processing_charge":"No"},{"publication":"Nature","date_updated":"2023-08-03T13:41:44Z","page":"611-615","article_type":"original","oa":1,"year":"2022","publisher":"Springer Nature","intvolume":"       609","isi":1,"type":"journal_article","language":[{"iso":"eng"}],"quality_controlled":"1","department":[{"_id":"JiFr"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","external_id":{"isi":["000848082900002"],"pmid":["35917925"]},"date_published":"2022-08-02T00:00:00Z","date_created":"2022-09-07T14:19:52Z","scopus_import":"1","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"publication_status":"published","acknowledgement":"We thank the Cryo-EM Center of the University of Science and Technology of China (USTC) and the Center for Biological Imaging (CBI), Institute of Biophysics, Chinese Academy of Science, for the EM facility support; we thank B. Zhu, X. Huang and all the other staff members for their technical support on cryo-EM data collection. We thank J. Ren for his technical support with the transport assays and M. Seeger for providing the sybody libraries. This work was supported by the Strategic Priority Research Program of Chinese Academy of Sciences (XDB 37020204 to D.L. and XDB37020103 to Linfeng Sun), National Natural Science Foundation of China (82151215 and 31870726 to D.L., 31900885 to X.L., and 31870732 to Linfeng Sun), Natural Science Foundation of Anhui Province (2008085MC90 to X.L. and 2008085J15 to Linfeng Sun), the Fundamental Research Funds for the Central Universities (WK9100000031 to Linfeng Sun), and the USTC Research Funds of the Double First-Class Initiative (YD9100002004 to Linfeng Sun). Linfeng Sun is supported by an Outstanding Young Scholar Award from the Qiu Shi Science and Technologies Foundation, and a Young Scholar Award from the Cyrus Tang Foundation.","status":"public","title":"Structural insights into auxin recognition and efflux by Arabidopsis PIN1","author":[{"first_name":"Z","full_name":"Yang, Z","last_name":"Yang"},{"first_name":"J","last_name":"Xia","full_name":"Xia, J"},{"first_name":"J","last_name":"Hong","full_name":"Hong, J"},{"full_name":"Zhang, C","last_name":"Zhang","first_name":"C"},{"last_name":"Wei","full_name":"Wei, H","first_name":"H"},{"first_name":"W","full_name":"Ying, W","last_name":"Ying"},{"last_name":"Sun","full_name":"Sun, C","first_name":"C"},{"last_name":"Sun","full_name":"Sun, L","first_name":"L"},{"full_name":"Mao, Y","last_name":"Mao","first_name":"Y"},{"first_name":"Y","full_name":"Gao, Y","last_name":"Gao"},{"full_name":"Tan, S","last_name":"Tan","first_name":"S"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Li, D","last_name":"Li","first_name":"D"},{"last_name":"Liu","full_name":"Liu, X","first_name":"X"},{"first_name":"L","last_name":"Sun","full_name":"Sun, L"}],"_id":"12054","abstract":[{"lang":"eng","text":"Polar auxin transport is unique to plants and coordinates their growth and development1,2. The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical localizations at the plasma membrane and drive polar auxin transport3,4; however, their structures and transport mechanisms remain largely unknown. Here, we report three inward-facing conformation structures of Arabidopsis thaliana PIN1: the apo state, bound to the natural auxin indole-3-acetic acid (IAA), and in complex with the polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). The transmembrane domain of PIN1 shares a conserved NhaA fold5. In the substrate-bound structure, IAA is coordinated by both hydrophobic stacking and hydrogen bonding. NPA competes with IAA for the same site at the intracellular pocket, but with a much higher affinity. These findings inform our understanding of the substrate recognition and transport mechanisms of PINs and set up a framework for future research on directional auxin transport, one of the most crucial processes underlying plant development."}],"issue":"7927","doi":"10.1038/s41586-022-05143-9","file":[{"success":1,"date_updated":"2022-09-08T08:02:54Z","access_level":"open_access","content_type":"application/pdf","checksum":"3136a585f8e1c7e73b5e1418b3d01898","file_name":"2022_Nature_Yang.pdf","file_size":32344580,"relation":"main_file","creator":"dernst","file_id":"12064","date_created":"2022-09-08T08:02:54Z"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","article_processing_charge":"No","volume":609,"citation":{"chicago":"Yang, Z, J Xia, J Hong, C Zhang, H Wei, W Ying, C Sun, et al. “Structural Insights into Auxin Recognition and Efflux by Arabidopsis PIN1.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-05143-9\">https://doi.org/10.1038/s41586-022-05143-9</a>.","mla":"Yang, Z., et al. “Structural Insights into Auxin Recognition and Efflux by Arabidopsis PIN1.” <i>Nature</i>, vol. 609, no. 7927, Springer Nature, 2022, pp. 611–15, doi:<a href=\"https://doi.org/10.1038/s41586-022-05143-9\">10.1038/s41586-022-05143-9</a>.","short":"Z. Yang, J. Xia, J. Hong, C. Zhang, H. Wei, W. Ying, C. Sun, L. Sun, Y. Mao, Y. Gao, S. Tan, J. Friml, D. Li, X. Liu, L. Sun, Nature 609 (2022) 611–615.","ama":"Yang Z, Xia J, Hong J, et al. Structural insights into auxin recognition and efflux by Arabidopsis PIN1. <i>Nature</i>. 2022;609(7927):611-615. doi:<a href=\"https://doi.org/10.1038/s41586-022-05143-9\">10.1038/s41586-022-05143-9</a>","ista":"Yang Z, Xia J, Hong J, Zhang C, Wei H, Ying W, Sun C, Sun L, Mao Y, Gao Y, Tan S, Friml J, Li D, Liu X, Sun L. 2022. Structural insights into auxin recognition and efflux by Arabidopsis PIN1. Nature. 609(7927), 611–615.","ieee":"Z. Yang <i>et al.</i>, “Structural insights into auxin recognition and efflux by Arabidopsis PIN1,” <i>Nature</i>, vol. 609, no. 7927. Springer Nature, pp. 611–615, 2022.","apa":"Yang, Z., Xia, J., Hong, J., Zhang, C., Wei, H., Ying, W., … Sun, L. (2022). Structural insights into auxin recognition and efflux by Arabidopsis PIN1. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-05143-9\">https://doi.org/10.1038/s41586-022-05143-9</a>"},"day":"02","month":"08","pmid":1,"ddc":["580"],"file_date_updated":"2022-09-08T08:02:54Z","oa_version":"Published Version"},{"month":"12","citation":{"chicago":"Valentini, Marco, Maksim Borovkov, Elsa Prada, Sara Martí-Sánchez, Marc Botifoll, Andrea C Hofmann, Jordi Arbiol, Ramón Aguado, Pablo San-Jose, and Georgios Katsaros. “Majorana-like Coulomb Spectroscopy in the Absence of Zero-Bias Peaks.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-05382-w\">https://doi.org/10.1038/s41586-022-05382-w</a>.","mla":"Valentini, Marco, et al. “Majorana-like Coulomb Spectroscopy in the Absence of Zero-Bias Peaks.” <i>Nature</i>, vol. 612, no. 7940, Springer Nature, 2022, pp. 442–47, doi:<a href=\"https://doi.org/10.1038/s41586-022-05382-w\">10.1038/s41586-022-05382-w</a>.","ama":"Valentini M, Borovkov M, Prada E, et al. Majorana-like Coulomb spectroscopy in the absence of zero-bias peaks. <i>Nature</i>. 2022;612(7940):442-447. doi:<a href=\"https://doi.org/10.1038/s41586-022-05382-w\">10.1038/s41586-022-05382-w</a>","short":"M. Valentini, M. Borovkov, E. Prada, S. Martí-Sánchez, M. Botifoll, A.C. Hofmann, J. Arbiol, R. Aguado, P. San-Jose, G. Katsaros, Nature 612 (2022) 442–447.","ista":"Valentini M, Borovkov M, Prada E, Martí-Sánchez S, Botifoll M, Hofmann AC, Arbiol J, Aguado R, San-Jose P, Katsaros G. 2022. Majorana-like Coulomb spectroscopy in the absence of zero-bias peaks. Nature. 612(7940), 442–447.","ieee":"M. Valentini <i>et al.</i>, “Majorana-like Coulomb spectroscopy in the absence of zero-bias peaks,” <i>Nature</i>, vol. 612, no. 7940. Springer Nature, pp. 442–447, 2022.","apa":"Valentini, M., Borovkov, M., Prada, E., Martí-Sánchez, S., Botifoll, M., Hofmann, A. C., … Katsaros, G. (2022). Majorana-like Coulomb spectroscopy in the absence of zero-bias peaks. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-05382-w\">https://doi.org/10.1038/s41586-022-05382-w</a>"},"day":"15","oa_version":"Preprint","arxiv":1,"volume":612,"article_processing_charge":"No","_id":"12118","title":"Majorana-like Coulomb spectroscopy in the absence of zero-bias peaks","author":[{"first_name":"Marco","id":"C0BB2FAC-D767-11E9-B658-BC13E6697425","last_name":"Valentini","full_name":"Valentini, Marco"},{"last_name":"Borovkov","full_name":"Borovkov, Maksim","first_name":"Maksim","id":"2ac7a0a2-3562-11eb-9256-fbd18ea55087"},{"first_name":"Elsa","full_name":"Prada, Elsa","last_name":"Prada"},{"first_name":"Sara","full_name":"Martí-Sánchez, Sara","last_name":"Martí-Sánchez"},{"full_name":"Botifoll, Marc","last_name":"Botifoll","first_name":"Marc"},{"last_name":"Hofmann","full_name":"Hofmann, Andrea C","first_name":"Andrea C","id":"340F461A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jordi","full_name":"Arbiol, Jordi","last_name":"Arbiol"},{"last_name":"Aguado","full_name":"Aguado, Ramón","first_name":"Ramón"},{"full_name":"San-Jose, Pablo","last_name":"San-Jose","first_name":"Pablo"},{"orcid":"0000-0001-8342-202X","full_name":"Katsaros, Georgios","last_name":"Katsaros","id":"38DB5788-F248-11E8-B48F-1D18A9856A87","first_name":"Georgios"}],"doi":"10.1038/s41586-022-05382-w","abstract":[{"text":"Hybrid semiconductor–superconductor devices hold great promise for realizing topological quantum computing with Majorana zero modes1,2,3,4,5. However, multiple claims of Majorana detection, based on either tunnelling6,7,8,9,10 or Coulomb blockade (CB) spectroscopy11,12, remain disputed. Here we devise an experimental protocol that allows us to perform both types of measurement on the same hybrid island by adjusting its charging energy via tunable junctions to the normal leads. This method reduces ambiguities of Majorana detections by checking the consistency between CB spectroscopy and zero-bias peaks in non-blockaded transport. Specifically, we observe junction-dependent, even–odd modulated, single-electron CB peaks in InAs/Al hybrid nanowires without concomitant low-bias peaks in tunnelling spectroscopy. We provide a theoretical interpretation of the experimental observations in terms of low-energy, longitudinally confined island states rather than overlapping Majorana modes. Our results highlight the importance of combined measurements on the same device for the identification of topological Majorana zero modes.","lang":"eng"}],"issue":"7940","project":[{"call_identifier":"H2020","name":"Majorana bound states in Ge/SiGe heterostructures","_id":"26A151DA-B435-11E9-9278-68D0E5697425","grant_number":"844511"}],"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"ec_funded":1,"status":"public","publication_status":"published","acknowledgement":"We thank P. Krogstrup for providing us with the NW materials. We thank A. Higginbotham, E. J. H. Lee, C. Marcus and S. Vaitiekėnas for helpful discussions and G. Steffensen for his input on the diffusive Little-Parks theory. This research was supported by the Scientific Service Units of ISTA through resources provided by the MIBA Machine Shop and the nanofabrication facility; the NOMIS Foundation; the CSIC Interdisciplinary Thematic Platform (PTI+) on Quantum Technologies (PTI-QTEP+). A.H. acknowledges support from H2020-MSCA-IF-2018/844511. ICN2 also acknowledges funding from Generalitat de Catalunya 2017 SGR 327. ICN2 is supported by the Severo Ochoa Program from Spanish MINECO (Grant no. SEV-2017-0706) and is funded by the CERCA Programme/Generalitat de Catalunya. Part of the present work has been performed in the framework of Universitat Autònoma de Barcelona Materials Science PhD programme. Authors acknowledge the use of instrumentation as well as the technical advice provided by the National Facility ELECMI ICTS, node ‘Laboratorio de Microscopías Avanzadas’ at University of Zaragoza. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 823717-ESTEEM3. This study was supported by MCIN with funding from European Union NextGenerationEU (PRTR-C17.I1) and Generalitat de Catalunya. This research is part of the CSIC programme for the Spanish Recovery, Transformation and Resilience Plan funded by the Recovery and Resilience Facility of the European Union, established by the Regulation (EU) 2020/2094. We thank support from Grant PGC2018-097018-BI00, project FlagERA TOPOGRAPH (PCI2018-093026) and project NANOGEN (PID2020-116093RB-C43), funded by MCIN/AEI/10.13039/501100011033/ and by ‘ERDF A way of making Europe’, by the European Union. M. Botifoll acknowledges support from SUR Generalitat de Catalunya and the EU Social Fund (project ref. 2020 FI 00103).","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","department":[{"_id":"GeKa"}],"date_created":"2023-01-12T11:56:45Z","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"}],"scopus_import":"1","external_id":{"arxiv":["2203.07829"],"isi":["000899725400001"]},"date_published":"2022-12-15T00:00:00Z","publisher":"Springer Nature","intvolume":"       612","main_file_link":[{"url":" https://doi.org/10.48550/arXiv.2203.07829","open_access":"1"}],"quality_controlled":"1","type":"journal_article","language":[{"iso":"eng"}],"isi":1,"oa":1,"keyword":["Multidisciplinary"],"year":"2022","date_updated":"2024-02-21T12:35:33Z","publication":"Nature","page":"442-447","article_type":"original","related_material":{"link":[{"relation":"press_release","description":"News on ISTA Website","url":"https://ista.ac.at/en/news/imposter-particles-revealed-and-explained/"}],"record":[{"status":"public","relation":"dissertation_contains","id":"13286"},{"relation":"research_data","status":"public","id":"12522"}]}},{"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"project":[{"name":"Structural characterization of E. coli complex I: an important mechanistic model","grant_number":"25541","_id":"238A0A5A-32DE-11EA-91FC-C7463DDC885E"},{"name":"Structure and mechanism of respiratory chain molecular machines","call_identifier":"H2020","_id":"627abdeb-2b32-11ec-9570-ec31a97243d3","grant_number":"101020697"}],"status":"public","publication_status":"published","acknowledgement":"This research was supported by the Scientific Service Units (SSU) of IST Austria through resources provided by the Electron Microscopy Facility (EMF), the Life Science Facility (LSF) and the IST high-performance computing cluster. We thank V.-V. Hodirnau from IST Austria EMF, M. Babiak from CEITEC for assistance with collecting cryo-EM data and A. Charnagalov for the assistance with protein purification. V.K. was a recipient of a DOC Fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology, Austria. V.K. and O.P. are funded by the ERC Advanced Grant 101020697 RESPICHAIN to L.S. This work was also supported by the Medical Research Council (UK).","ec_funded":1,"title":"A universal coupling mechanism of respiratory complex I","author":[{"id":"4D62F2A6-F248-11E8-B48F-1D18A9856A87","first_name":"Vladyslav","full_name":"Kravchuk, Vladyslav","last_name":"Kravchuk"},{"id":"5D8C9660-5D49-11EA-8188-567B3DDC885E","first_name":"Olga","full_name":"Petrova, Olga","last_name":"Petrova"},{"id":"37233050-F248-11E8-B48F-1D18A9856A87","first_name":"Domen","full_name":"Kampjut, Domen","last_name":"Kampjut"},{"first_name":"Anna","last_name":"Wojciechowska-Bason","full_name":"Wojciechowska-Bason, Anna"},{"last_name":"Breese","full_name":"Breese, Zara","first_name":"Zara"},{"full_name":"Sazanov, Leonid A","last_name":"Sazanov","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","first_name":"Leonid A","orcid":"0000-0002-0977-7989"}],"_id":"12138","abstract":[{"text":"Complex I is the first enzyme in the respiratory chain, which is responsible for energy production in mitochondria and bacteria1. Complex I couples the transfer of two electrons from NADH to quinone and the translocation of four protons across the membrane2, but the coupling mechanism remains contentious. Here we present cryo-electron microscopy structures of Escherichia coli complex I (EcCI) in different redox states, including catalytic turnover. EcCI exists mostly in the open state, in which the quinone cavity is exposed to the cytosol, allowing access for water molecules, which enable quinone movements. Unlike the mammalian paralogues3, EcCI can convert to the closed state only during turnover, showing that closed and open states are genuine turnover intermediates. The open-to-closed transition results in the tightly engulfed quinone cavity being connected to the central axis of the membrane arm, a source of substrate protons. Consistently, the proportion of the closed state increases with increasing pH. We propose a detailed but straightforward and robust mechanism comprising a ‘domino effect’ series of proton transfers and electrostatic interactions: the forward wave (‘dominoes stacking’) primes the pump, and the reverse wave (‘dominoes falling’) results in the ejection of all pumped protons from the distal subunit NuoL. This mechanism explains why protons exit exclusively from the NuoL subunit and is supported by our mutagenesis data. We contend that this is a universal coupling mechanism of complex I and related enzymes.","lang":"eng"}],"issue":"7928","doi":"10.1038/s41586-022-05199-7","file":[{"date_updated":"2023-05-30T17:05:31Z","success":1,"content_type":"application/pdf","checksum":"d42a93e24f59e883ef0b5429832391d0","access_level":"open_access","relation":"main_file","creator":"lsazanov","file_size":1425655,"file_name":"EcCxI_manuscript_rev3_noSI_updated_withFigs_opt.pdf","date_created":"2023-05-30T17:05:31Z","file_id":"13104"},{"file_name":"EcCxI_manuscript_rev3_SI_All_opt_upd.pdf","creator":"lsazanov","file_size":9842513,"relation":"main_file","success":1,"date_updated":"2023-05-30T17:07:05Z","access_level":"open_access","content_type":"application/pdf","checksum":"5422bc0a73b3daadafa262c7ea6deae3","file_id":"13105","date_created":"2023-05-30T17:07:05Z"}],"has_accepted_license":"1","article_processing_charge":"No","volume":609,"citation":{"short":"V. Kravchuk, O. Petrova, D. Kampjut, A. Wojciechowska-Bason, Z. Breese, L.A. Sazanov, Nature 609 (2022) 808–814.","ama":"Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov LA. A universal coupling mechanism of respiratory complex I. <i>Nature</i>. 2022;609(7928):808-814. doi:<a href=\"https://doi.org/10.1038/s41586-022-05199-7\">10.1038/s41586-022-05199-7</a>","ista":"Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov LA. 2022. A universal coupling mechanism of respiratory complex I. Nature. 609(7928), 808–814.","apa":"Kravchuk, V., Petrova, O., Kampjut, D., Wojciechowska-Bason, A., Breese, Z., &#38; Sazanov, L. A. (2022). A universal coupling mechanism of respiratory complex I. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-05199-7\">https://doi.org/10.1038/s41586-022-05199-7</a>","ieee":"V. Kravchuk, O. Petrova, D. Kampjut, A. Wojciechowska-Bason, Z. Breese, and L. A. Sazanov, “A universal coupling mechanism of respiratory complex I,” <i>Nature</i>, vol. 609, no. 7928. Springer Nature, pp. 808–814, 2022.","chicago":"Kravchuk, Vladyslav, Olga Petrova, Domen Kampjut, Anna Wojciechowska-Bason, Zara Breese, and Leonid A Sazanov. “A Universal Coupling Mechanism of Respiratory Complex I.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-05199-7\">https://doi.org/10.1038/s41586-022-05199-7</a>.","mla":"Kravchuk, Vladyslav, et al. “A Universal Coupling Mechanism of Respiratory Complex I.” <i>Nature</i>, vol. 609, no. 7928, Springer Nature, 2022, pp. 808–14, doi:<a href=\"https://doi.org/10.1038/s41586-022-05199-7\">10.1038/s41586-022-05199-7</a>."},"day":"22","file_date_updated":"2023-05-30T17:07:05Z","pmid":1,"ddc":["572"],"month":"09","oa_version":"Submitted Version","publication":"Nature","date_updated":"2023-08-04T08:54:52Z","related_material":{"link":[{"relation":"erratum","url":"https://doi.org/10.1038/s41586-022-05457-8"},{"relation":"press_release","url":"https://ista.ac.at/en/news/proton-dominos-kick-off-life/","description":"News on ISTA website"}],"record":[{"id":"12781","status":"public","relation":"dissertation_contains"}]},"page":"808-814","article_type":"original","oa":1,"year":"2022","keyword":["Multidisciplinary"],"publisher":"Springer Nature","intvolume":"       609","isi":1,"language":[{"iso":"eng"}],"type":"journal_article","quality_controlled":"1","department":[{"_id":"LeSa"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","external_id":{"isi":["000854788200001"],"pmid":["36104567"]},"date_published":"2022-09-22T00:00:00Z","date_created":"2023-01-12T12:04:33Z","scopus_import":"1","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"ScienComp"}]},{"page":"133-138","article_type":"original","publication":"Nature","date_updated":"2023-10-03T11:04:53Z","year":"2022","oa":1,"isi":1,"type":"journal_article","language":[{"iso":"eng"}],"quality_controlled":"1","publisher":"Springer Nature","intvolume":"       611","main_file_link":[{"open_access":"1","url":"http://wrap.warwick.ac.uk/168325/1/WRAP-denylate-cyclase-activity-TIR1-AFB-auxin-receptors-root-growth-22.pdf"}],"external_id":{"isi":["000875061600013"],"pmid":["36289340"]},"date_published":"2022-11-03T00:00:00Z","date_created":"2023-01-12T12:06:05Z","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"scopus_import":"1","department":[{"_id":"JiFr"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"This research was supported by the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) of IST Austria. We thank C. Gehring for suggestions and advice; and K. U. Torii and G. Stacey for seeds and plasmids. This project was funded by a European Research Council Advanced Grant (ETAP-742985). M.F.K. and R.N. acknowledge the support of the EU MSCA-IF project CrysPINs (792329). M.K. was supported by the project POWR.03.05.00-00-Z302/17 Universitas Copernicana Thoruniensis in Futuro–IDS “Academia Copernicana”. CIDG acknowledges support from UKRI under Future Leaders Fellowship grant number MR/T020652/1.","publication_status":"published","status":"public","ec_funded":1,"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"abstract":[{"lang":"eng","text":"The phytohormone auxin is the major coordinative signal in plant development1, mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin, they associate with Aux/IAA transcriptional repressors and target them for degradation via ubiquitination2,3. Here we identify adenylate cyclase (AC) activity as an additional function of TIR1/AFB receptors across land plants. Auxin, together with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC motif of the TIR1 C-terminal region, all of which abolish the AC activity, each render TIR1 ineffective in mediating gravitropism and sustained auxin-induced root growth inhibition, and also affect auxin-induced transcriptional regulation. These results highlight the importance of TIR1/AFB AC activity in canonical auxin signalling. They also identify a unique phytohormone receptor cassette combining F-box and AC motifs, and the role of cAMP as a second messenger in plants."}],"issue":"7934","doi":"10.1038/s41586-022-05369-7","title":"Adenylate cyclase activity of TIR1/AFB auxin receptors in plants","author":[{"full_name":"Qi, Linlin","last_name":"Qi","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","first_name":"Linlin","orcid":"0000-0001-5187-8401"},{"first_name":"Mateusz","full_name":"Kwiatkowski, Mateusz","last_name":"Kwiatkowski"},{"last_name":"Chen","full_name":"Chen, Huihuang","first_name":"Huihuang","id":"83c96512-15b2-11ec-abd3-b7eede36184f"},{"orcid":"0000-0001-8295-2926","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","first_name":"Lukas","full_name":"Hörmayer, Lukas","last_name":"Hörmayer"},{"first_name":"Scott A","id":"2D99FE6A-F248-11E8-B48F-1D18A9856A87","last_name":"Sinclair","full_name":"Sinclair, Scott A","orcid":"0000-0002-4566-0593"},{"id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","first_name":"Minxia","full_name":"Zou, Minxia","last_name":"Zou"},{"last_name":"del Genio","full_name":"del Genio, Charo I.","first_name":"Charo I."},{"first_name":"Martin F.","full_name":"Kubeš, Martin F.","last_name":"Kubeš"},{"full_name":"Napier, Richard","last_name":"Napier","first_name":"Richard"},{"full_name":"Jaworski, Krzysztof","last_name":"Jaworski","first_name":"Krzysztof"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"_id":"12144","article_processing_charge":"No","volume":611,"oa_version":"Submitted Version","citation":{"mla":"Qi, Linlin, et al. “Adenylate Cyclase Activity of TIR1/AFB Auxin Receptors in Plants.” <i>Nature</i>, vol. 611, no. 7934, Springer Nature, 2022, pp. 133–38, doi:<a href=\"https://doi.org/10.1038/s41586-022-05369-7\">10.1038/s41586-022-05369-7</a>.","chicago":"Qi, Linlin, Mateusz Kwiatkowski, Huihuang Chen, Lukas Hörmayer, Scott A Sinclair, Minxia Zou, Charo I. del Genio, et al. “Adenylate Cyclase Activity of TIR1/AFB Auxin Receptors in Plants.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-05369-7\">https://doi.org/10.1038/s41586-022-05369-7</a>.","ieee":"L. Qi <i>et al.</i>, “Adenylate cyclase activity of TIR1/AFB auxin receptors in plants,” <i>Nature</i>, vol. 611, no. 7934. Springer Nature, pp. 133–138, 2022.","apa":"Qi, L., Kwiatkowski, M., Chen, H., Hörmayer, L., Sinclair, S. A., Zou, M., … Friml, J. (2022). Adenylate cyclase activity of TIR1/AFB auxin receptors in plants. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-05369-7\">https://doi.org/10.1038/s41586-022-05369-7</a>","short":"L. Qi, M. Kwiatkowski, H. Chen, L. Hörmayer, S.A. Sinclair, M. Zou, C.I. del Genio, M.F. Kubeš, R. Napier, K. Jaworski, J. Friml, Nature 611 (2022) 133–138.","ista":"Qi L, Kwiatkowski M, Chen H, Hörmayer L, Sinclair SA, Zou M, del Genio CI, Kubeš MF, Napier R, Jaworski K, Friml J. 2022. Adenylate cyclase activity of TIR1/AFB auxin receptors in plants. Nature. 611(7934), 133–138.","ama":"Qi L, Kwiatkowski M, Chen H, et al. Adenylate cyclase activity of TIR1/AFB auxin receptors in plants. <i>Nature</i>. 2022;611(7934):133-138. doi:<a href=\"https://doi.org/10.1038/s41586-022-05369-7\">10.1038/s41586-022-05369-7</a>"},"day":"03","pmid":1,"month":"11"},{"pmid":1,"month":"07","day":"13","citation":{"chicago":"Azkanaz, Maria, Bernat Corominas-Murtra, Saskia I. J. Ellenbroek, Lotte Bruens, Anna T. Webb, Dimitrios Laskaris, Koen C. Oost, et al. “Retrograde Movements Determine Effective Stem Cell Numbers in the Intestine.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-04962-0\">https://doi.org/10.1038/s41586-022-04962-0</a>.","mla":"Azkanaz, Maria, et al. “Retrograde Movements Determine Effective Stem Cell Numbers in the Intestine.” <i>Nature</i>, vol. 607, no. 7919, Springer Nature, 2022, pp. 548–54, doi:<a href=\"https://doi.org/10.1038/s41586-022-04962-0\">10.1038/s41586-022-04962-0</a>.","ieee":"M. Azkanaz <i>et al.</i>, “Retrograde movements determine effective stem cell numbers in the intestine,” <i>Nature</i>, vol. 607, no. 7919. Springer Nature, pp. 548–554, 2022.","apa":"Azkanaz, M., Corominas-Murtra, B., Ellenbroek, S. I. J., Bruens, L., Webb, A. T., Laskaris, D., … van Rheenen, J. (2022). Retrograde movements determine effective stem cell numbers in the intestine. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-04962-0\">https://doi.org/10.1038/s41586-022-04962-0</a>","ama":"Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, et al. Retrograde movements determine effective stem cell numbers in the intestine. <i>Nature</i>. 2022;607(7919):548-554. doi:<a href=\"https://doi.org/10.1038/s41586-022-04962-0\">10.1038/s41586-022-04962-0</a>","ista":"Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, Bruens L, Webb AT, Laskaris D, Oost KC, Lafirenze SJA, Annusver K, Messal HA, Iqbal S, Flanagan DJ, Huels DJ, Rojas-Rodríguez F, Vizoso M, Kasper M, Sansom OJ, Snippert HJ, Liberali P, Simons BD, Katajisto P, Hannezo EB, van Rheenen J. 2022. Retrograde movements determine effective stem cell numbers in the intestine. Nature. 607(7919), 548–554.","short":"M. Azkanaz, B. Corominas-Murtra, S.I.J. Ellenbroek, L. Bruens, A.T. Webb, D. Laskaris, K.C. Oost, S.J.A. Lafirenze, K. Annusver, H.A. Messal, S. Iqbal, D.J. Flanagan, D.J. Huels, F. Rojas-Rodríguez, M. Vizoso, M. Kasper, O.J. Sansom, H.J. Snippert, P. Liberali, B.D. Simons, P. Katajisto, E.B. Hannezo, J. van Rheenen, Nature 607 (2022) 548–554."},"oa_version":"Submitted Version","volume":607,"article_processing_charge":"No","_id":"12274","author":[{"first_name":"Maria","full_name":"Azkanaz, Maria","last_name":"Azkanaz"},{"full_name":"Corominas-Murtra, Bernat","last_name":"Corominas-Murtra","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","first_name":"Bernat","orcid":"0000-0001-9806-5643"},{"last_name":"Ellenbroek","full_name":"Ellenbroek, Saskia I. J.","first_name":"Saskia I. J."},{"full_name":"Bruens, Lotte","last_name":"Bruens","first_name":"Lotte"},{"last_name":"Webb","full_name":"Webb, Anna T.","first_name":"Anna T."},{"full_name":"Laskaris, Dimitrios","last_name":"Laskaris","first_name":"Dimitrios"},{"first_name":"Koen C.","last_name":"Oost","full_name":"Oost, Koen C."},{"first_name":"Simona J. A.","last_name":"Lafirenze","full_name":"Lafirenze, Simona J. A."},{"first_name":"Karl","last_name":"Annusver","full_name":"Annusver, Karl"},{"last_name":"Messal","full_name":"Messal, Hendrik A.","first_name":"Hendrik A."},{"first_name":"Sharif","last_name":"Iqbal","full_name":"Iqbal, Sharif"},{"last_name":"Flanagan","full_name":"Flanagan, Dustin J.","first_name":"Dustin J."},{"first_name":"David J.","full_name":"Huels, David J.","last_name":"Huels"},{"first_name":"Felipe","last_name":"Rojas-Rodríguez","full_name":"Rojas-Rodríguez, Felipe"},{"first_name":"Miguel","full_name":"Vizoso, Miguel","last_name":"Vizoso"},{"first_name":"Maria","last_name":"Kasper","full_name":"Kasper, Maria"},{"full_name":"Sansom, Owen J.","last_name":"Sansom","first_name":"Owen J."},{"first_name":"Hugo J.","last_name":"Snippert","full_name":"Snippert, Hugo J."},{"first_name":"Prisca","last_name":"Liberali","full_name":"Liberali, Prisca"},{"last_name":"Simons","full_name":"Simons, Benjamin D.","first_name":"Benjamin D."},{"last_name":"Katajisto","full_name":"Katajisto, Pekka","first_name":"Pekka"},{"orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B"},{"first_name":"Jacco","last_name":"van Rheenen","full_name":"van Rheenen, Jacco"}],"title":"Retrograde movements determine effective stem cell numbers in the intestine","doi":"10.1038/s41586-022-04962-0","issue":"7919","abstract":[{"text":"The morphology and functionality of the epithelial lining differ along the intestinal tract, but tissue renewal at all sites is driven by stem cells at the base of crypts1,2,3. Whether stem cell numbers and behaviour vary at different sites is unknown. Here we show using intravital microscopy that, despite similarities in the number and distribution of proliferative cells with an Lgr5 signature in mice, small intestinal crypts contain twice as many effective stem cells as large intestinal crypts. We find that, although passively displaced by a conveyor-belt-like upward movement, small intestinal cells positioned away from the crypt base can function as long-term effective stem cells owing to Wnt-dependent retrograde cellular movement. By contrast, the near absence of retrograde movement in the large intestine restricts cell repositioning, leading to a reduction in effective stem cell number. Moreover, after suppression of the retrograde movement in the small intestine, the number of effective stem cells is reduced, and the rate of monoclonal conversion of crypts is accelerated. Together, these results show that the number of effective stem cells is determined by active retrograde movement, revealing a new channel of stem cell regulation that can be experimentally and pharmacologically manipulated.","lang":"eng"}],"project":[{"call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288"}],"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"ec_funded":1,"status":"public","acknowledgement":"We thank the members of the van Rheenen laboratory for reading the manuscript, and the members of the bioimaging, FACS and animal facility of the NKI for experimental support. We acknowledge the staff at the MedH Flow Cytometry core facility, Karolinska Institutet, and LCI facility/Nikon Center of Excellence, Karolinska Institutet. This work was financially supported by the Netherlands Organization of Scientific Research NWO (Veni grant 863.15.011 to S.I.J.E. and Vici grant 09150182110004 to J.v.R.) and the CancerGenomics.nl (Netherlands Organisation for Scientific Research) program (to J.v.R.) the Doctor Josef Steiner Foundation (to J.v.R). B.D.S. acknowledges funding from the Royal Society E.P. Abraham Research Professorship (RP\\R1\\180165) and the Wellcome Trust (098357/Z/12/Z and 219478/Z/19/Z). B.C.-M. acknowledges the support of the field of excellence ‘Complexity of life in basic research and innovation’ of the University of Graz. O.J.S. and their laboratory acknowledge CRUK core funding to the CRUK Beatson Institute (A17196 and A31287) and CRUK core funding to the Sansom laboratory (A21139). P.K. and their laboratory are supported by grants from the Swedish Research Council (2018-03078), Cancerfonden (190634), Academy of Finland Centre of Excellence (266869, 304591 and 320185) and the Jane and Aatos Erkko Foundation. P.L. has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 758617). E.H. acknowledges funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 851288).","publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"EdHa"}],"scopus_import":"1","date_created":"2023-01-16T10:01:29Z","date_published":"2022-07-13T00:00:00Z","external_id":{"isi":["000824430000004"],"pmid":["35831497"]},"main_file_link":[{"url":"https://helda.helsinki.fi/items/94433455-4854-45c0-9de8-7326caea8780","open_access":"1"}],"intvolume":"       607","publisher":"Springer Nature","language":[{"iso":"eng"}],"quality_controlled":"1","type":"journal_article","isi":1,"oa":1,"keyword":["Multidisciplinary"],"year":"2022","date_updated":"2023-10-03T11:16:30Z","publication":"Nature","article_type":"original","page":"548-554","related_material":{"link":[{"relation":"software","url":"https://github.com/JaccovanRheenenLab/Retrograde_movement_Azkanaz_Nature_2022"}]}},{"date_updated":"2023-11-07T08:16:09Z","publication":"Nature","page":"575-581","article_type":"original","oa":1,"year":"2022","publisher":"Springer Nature","intvolume":"       609","type":"journal_article","language":[{"iso":"eng"}],"quality_controlled":"1","isi":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"EvBe"},{"_id":"EM-Fac"}],"date_created":"2023-01-16T10:04:48Z","scopus_import":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"date_published":"2022-09-15T00:00:00Z","external_id":{"pmid":["36071161"],"isi":["000851357500002"]},"project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"call_identifier":"FWF","name":"RNA-directed DNA methylation in plant development","_id":"262EF96E-B435-11E9-9278-68D0E5697425","grant_number":"P29988"}],"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"ec_funded":1,"acknowledgement":"We acknowledge K. Kubiasová for excellent technical assistance, J. Neuhold, A. Lehner and A. Sedivy for technical assistance with protein production and purification at Vienna Biocenter Core Facilities; Creoptix for performing GCI; and the Bioimaging, Electron Microscopy and Life Science Facilities at ISTA, the Plant Sciences Core Facility of CEITEC Masaryk University, the Core Facility CELLIM (MEYS CR, LM2018129 Czech-BioImaging) and J. Sprakel for their assistance. J.F. is grateful to R. Napier for many insightful suggestions and support. We thank all past and present members of the Friml group for their support and for other contributions to this effort to clarify the controversial role of ABP1 over the past seven years. The project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 742985 to J.F. and 833867 to D.W.); the Austrian Science Fund (FWF; P29988 to J.F.); the Netherlands Organization for Scientific Research (NWO; VICI grant 865.14.001 to D.W. and VENI grant VI.Veni.212.003 to A.K.); the Ministry of Education, Science and Technological Development of the Republic of Serbia (contract no. 451-03-68/2022-14/200053 to B.D.Ž.); and the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and 20H05910).","publication_status":"published","status":"public","_id":"12291","title":"ABP1–TMK auxin perception for global phosphorylation and auxin canalization","author":[{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml"},{"orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","first_name":"Michelle C"},{"orcid":"0000-0003-4783-1752","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","first_name":"Zuzana","full_name":"Gelová, Zuzana","last_name":"Gelová"},{"full_name":"Johnson, Alexander J","last_name":"Johnson","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J","orcid":"0000-0002-2739-8843"},{"first_name":"Ewa","last_name":"Mazur","full_name":"Mazur, Ewa"},{"last_name":"Monzer","full_name":"Monzer, Aline","first_name":"Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425"},{"last_name":"Rodriguez Solovey","full_name":"Rodriguez Solovey, Lesia","first_name":"Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7244-7237"},{"full_name":"Roosjen, Mark","last_name":"Roosjen","first_name":"Mark"},{"first_name":"Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten","full_name":"Verstraeten, Inge","orcid":"0000-0001-7241-2328"},{"full_name":"Živanović, Branka D.","last_name":"Živanović","first_name":"Branka D."},{"last_name":"Zou","full_name":"Zou, Minxia","first_name":"Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9"},{"last_name":"Fiedler","full_name":"Fiedler, Lukas","first_name":"Lukas","id":"7c417475-8972-11ed-ae7b-8b674ca26986"},{"first_name":"Caterina","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","last_name":"Giannini","full_name":"Giannini, Caterina"},{"first_name":"Peter","last_name":"Grones","full_name":"Grones, Peter"},{"id":"45A71A74-F248-11E8-B48F-1D18A9856A87","first_name":"Mónika","full_name":"Hrtyan, Mónika","last_name":"Hrtyan"},{"orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter"},{"first_name":"Andre","full_name":"Kuhn, Andre","last_name":"Kuhn"},{"last_name":"Narasimhan","full_name":"Narasimhan, Madhumitha","first_name":"Madhumitha","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8600-0671"},{"full_name":"Randuch, Marek","last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","first_name":"Marek"},{"first_name":"Nikola","full_name":"Rýdza, Nikola","last_name":"Rýdza"},{"first_name":"Koji","last_name":"Takahashi","full_name":"Takahashi, Koji"},{"orcid":"0000-0002-0471-8285","first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan","full_name":"Tan, Shutang"},{"first_name":"Anastasiia","id":"e3736151-106c-11ec-b916-c2558e2762c6","last_name":"Teplova","full_name":"Teplova, Anastasiia"},{"full_name":"Kinoshita, Toshinori","last_name":"Kinoshita","first_name":"Toshinori"},{"full_name":"Weijers, Dolf","last_name":"Weijers","first_name":"Dolf"},{"first_name":"Hana","full_name":"Rakusová, Hana","last_name":"Rakusová"}],"doi":"10.1038/s41586-022-05187-x","abstract":[{"lang":"eng","text":"The phytohormone auxin triggers transcriptional reprogramming through a well-characterized perception machinery in the nucleus. By contrast, mechanisms that underlie fast effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required for the auxin-induced ultrafast global phospho-response and for downstream processes that include the activation of H+-ATPase and accelerated cytoplasmic streaming. abp1 and tmk mutants cannot establish auxin-transporting channels and show defective auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that lacks the capacity to bind auxin is unable to complement these defects in abp1 mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface signalling, which mediates the global phospho-response and auxin canalization."}],"issue":"7927","file":[{"success":1,"date_updated":"2023-11-02T17:12:37Z","access_level":"open_access","checksum":"a6055c606aefb900bf62ae3e7d15f921","content_type":"application/pdf","file_name":"Friml Nature 2022_merged.pdf","creator":"amally","relation":"main_file","file_size":79774945,"file_id":"14483","date_created":"2023-11-02T17:12:37Z"}],"article_processing_charge":"No","volume":609,"has_accepted_license":"1","month":"09","pmid":1,"file_date_updated":"2023-11-02T17:12:37Z","ddc":["580"],"citation":{"mla":"Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>, vol. 609, no. 7927, Springer Nature, 2022, pp. 575–81, doi:<a href=\"https://doi.org/10.1038/s41586-022-05187-x\">10.1038/s41586-022-05187-x</a>.","chicago":"Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-05187-x\">https://doi.org/10.1038/s41586-022-05187-x</a>.","ieee":"J. Friml <i>et al.</i>, “ABP1–TMK auxin perception for global phosphorylation and auxin canalization,” <i>Nature</i>, vol. 609, no. 7927. Springer Nature, pp. 575–581, 2022.","apa":"Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A., … Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-05187-x\">https://doi.org/10.1038/s41586-022-05187-x</a>","ama":"Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. 2022;609(7927):575-581. doi:<a href=\"https://doi.org/10.1038/s41586-022-05187-x\">10.1038/s41586-022-05187-x</a>","ista":"Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K, Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581.","short":"J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini, P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza, K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature 609 (2022) 575–581."},"day":"15","oa_version":"Submitted Version"},{"doi":"10.1038/s41586-022-05386-6","issue":"7936","abstract":[{"text":"Sperm chromatin is typically transformed by protamines into a compact and transcriptionally inactive state1,2. Sperm cells of flowering plants lack protamines, yet they have small, transcriptionally active nuclei with chromatin condensed through an unknown mechanism3,4. Here we show that a histone variant, H2B.8, mediates sperm chromatin and nuclear condensation in Arabidopsis thaliana. Loss of H2B.8 causes enlarged sperm nuclei with dispersed chromatin, whereas ectopic expression in somatic cells produces smaller nuclei with aggregated chromatin. This result demonstrates that H2B.8 is sufficient for chromatin condensation. H2B.8 aggregates transcriptionally inactive AT-rich chromatin into phase-separated condensates, which facilitates nuclear compaction without reducing transcription. Reciprocal crosses show that mutation of h2b.8 reduces male transmission, which suggests that H2B.8-mediated sperm compaction is important for fertility. Altogether, our results reveal a new mechanism of nuclear compaction through global aggregation of unexpressed chromatin. We propose that H2B.8 is an evolutionary innovation of flowering plants that achieves nuclear condensation compatible with active transcription.","lang":"eng"}],"_id":"12671","author":[{"last_name":"Buttress","full_name":"Buttress, Toby","first_name":"Toby"},{"last_name":"He","full_name":"He, Shengbo","first_name":"Shengbo"},{"full_name":"Wang, Liang","last_name":"Wang","first_name":"Liang"},{"last_name":"Zhou","full_name":"Zhou, Shaoli","first_name":"Shaoli"},{"last_name":"Saalbach","full_name":"Saalbach, Gerhard","first_name":"Gerhard"},{"first_name":"Martin","full_name":"Vickers, Martin","last_name":"Vickers"},{"first_name":"Guohong","last_name":"Li","full_name":"Li, Guohong"},{"first_name":"Pilong","last_name":"Li","full_name":"Li, Pilong"},{"orcid":"0000-0002-4008-1234","first_name":"Xiaoqi","id":"e0164712-22ee-11ed-b12a-d80fcdf35958","last_name":"Feng","full_name":"Feng, Xiaoqi"}],"title":"Histone H2B.8 compacts flowering plant sperm through chromatin phase separation","publication_status":"published","status":"public","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"oa_version":"Published Version","month":"11","pmid":1,"day":"17","citation":{"chicago":"Buttress, Toby, Shengbo He, Liang Wang, Shaoli Zhou, Gerhard Saalbach, Martin Vickers, Guohong Li, Pilong Li, and Xiaoqi Feng. “Histone H2B.8 Compacts Flowering Plant Sperm through Chromatin Phase Separation.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-05386-6\">https://doi.org/10.1038/s41586-022-05386-6</a>.","mla":"Buttress, Toby, et al. “Histone H2B.8 Compacts Flowering Plant Sperm through Chromatin Phase Separation.” <i>Nature</i>, vol. 611, no. 7936, Springer Nature, 2022, pp. 614–22, doi:<a href=\"https://doi.org/10.1038/s41586-022-05386-6\">10.1038/s41586-022-05386-6</a>.","ista":"Buttress T, He S, Wang L, Zhou S, Saalbach G, Vickers M, Li G, Li P, Feng X. 2022. Histone H2B.8 compacts flowering plant sperm through chromatin phase separation. Nature. 611(7936), 614–622.","short":"T. Buttress, S. He, L. Wang, S. Zhou, G. Saalbach, M. Vickers, G. Li, P. Li, X. Feng, Nature 611 (2022) 614–622.","ama":"Buttress T, He S, Wang L, et al. Histone H2B.8 compacts flowering plant sperm through chromatin phase separation. <i>Nature</i>. 2022;611(7936):614-622. doi:<a href=\"https://doi.org/10.1038/s41586-022-05386-6\">10.1038/s41586-022-05386-6</a>","ieee":"T. Buttress <i>et al.</i>, “Histone H2B.8 compacts flowering plant sperm through chromatin phase separation,” <i>Nature</i>, vol. 611, no. 7936. Springer Nature, pp. 614–622, 2022.","apa":"Buttress, T., He, S., Wang, L., Zhou, S., Saalbach, G., Vickers, M., … Feng, X. (2022). Histone H2B.8 compacts flowering plant sperm through chromatin phase separation. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-05386-6\">https://doi.org/10.1038/s41586-022-05386-6</a>"},"article_processing_charge":"No","volume":611,"year":"2022","oa":1,"article_type":"original","page":"614-622","date_updated":"2023-05-08T10:59:22Z","publication":"Nature","scopus_import":"1","date_created":"2023-02-23T09:17:05Z","extern":"1","external_id":{"pmid":["36323776"]},"date_published":"2022-11-17T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"XiFe"}],"quality_controlled":"1","language":[{"iso":"eng"}],"type":"journal_article","intvolume":"       611","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41586-022-05386-6"}],"publisher":"Springer Nature"},{"date_published":"2021-06-02T00:00:00Z","external_id":{"isi":["000657238100003"],"pmid":["34079129"]},"scopus_import":"1","date_created":"2021-06-13T22:01:33Z","department":[{"_id":"PeJo"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","isi":1,"language":[{"iso":"eng"}],"quality_controlled":"1","type":"journal_article","intvolume":"       594","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41586-021-03613-0"}],"publisher":"Springer Nature","year":"2021","oa":1,"article_type":"original","page":"454-458","publication":"Nature","date_updated":"2023-08-08T13:59:51Z","oa_version":"Published Version","day":"02","citation":{"apa":"Zhang, D., Watson, J., Matthews, P. M., Cais, O., &#38; Greger, I. H. (2021). Gating and modulation of a hetero-octameric AMPA glutamate receptor. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-021-03613-0\">https://doi.org/10.1038/s41586-021-03613-0</a>","ieee":"D. Zhang, J. Watson, P. M. Matthews, O. Cais, and I. H. Greger, “Gating and modulation of a hetero-octameric AMPA glutamate receptor,” <i>Nature</i>, vol. 594. Springer Nature, pp. 454–458, 2021.","ama":"Zhang D, Watson J, Matthews PM, Cais O, Greger IH. Gating and modulation of a hetero-octameric AMPA glutamate receptor. <i>Nature</i>. 2021;594:454-458. doi:<a href=\"https://doi.org/10.1038/s41586-021-03613-0\">10.1038/s41586-021-03613-0</a>","ista":"Zhang D, Watson J, Matthews PM, Cais O, Greger IH. 2021. Gating and modulation of a hetero-octameric AMPA glutamate receptor. Nature. 594, 454–458.","short":"D. Zhang, J. Watson, P.M. Matthews, O. Cais, I.H. Greger, Nature 594 (2021) 454–458.","chicago":"Zhang, Danyang, Jake Watson, Peter M. Matthews, Ondrej Cais, and Ingo H. Greger. “Gating and Modulation of a Hetero-Octameric AMPA Glutamate Receptor.” <i>Nature</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41586-021-03613-0\">https://doi.org/10.1038/s41586-021-03613-0</a>.","mla":"Zhang, Danyang, et al. “Gating and Modulation of a Hetero-Octameric AMPA Glutamate Receptor.” <i>Nature</i>, vol. 594, Springer Nature, 2021, pp. 454–58, doi:<a href=\"https://doi.org/10.1038/s41586-021-03613-0\">10.1038/s41586-021-03613-0</a>."},"month":"06","pmid":1,"article_processing_charge":"No","volume":594,"abstract":[{"lang":"eng","text":"AMPA receptors (AMPARs) mediate the majority of excitatory transmission in the brain and enable the synaptic plasticity that underlies learning1. A diverse array of AMPAR signalling complexes are established by receptor auxiliary subunits, which associate with the AMPAR in various combinations to modulate trafficking, gating and synaptic strength2. However, their mechanisms of action are poorly understood. Here we determine cryo-electron microscopy structures of the heteromeric GluA1–GluA2 receptor assembled with both TARP-γ8 and CNIH2, the predominant AMPAR complex in the forebrain, in both resting and active states. Two TARP-γ8 and two CNIH2 subunits insert at distinct sites beneath the ligand-binding domains of the receptor, with site-specific lipids shaping each interaction and affecting the gating regulation of the AMPARs. Activation of the receptor leads to asymmetry between GluA1 and GluA2 along the ion conduction path and an outward expansion of the channel triggers counter-rotations of both auxiliary subunit pairs, promoting the active-state conformation. In addition, both TARP-γ8 and CNIH2 pivot towards the pore exit upon activation, extending their reach for cytoplasmic receptor elements. CNIH2 achieves this through its uniquely extended M2 helix, which has transformed this endoplasmic reticulum-export factor into a powerful AMPAR modulator that is capable of providing hippocampal pyramidal neurons with their integrative synaptic properties. "}],"doi":"10.1038/s41586-021-03613-0","author":[{"first_name":"Danyang","full_name":"Zhang, Danyang","last_name":"Zhang"},{"last_name":"Watson","full_name":"Watson, Jake","first_name":"Jake","id":"63836096-4690-11EA-BD4E-32803DDC885E","orcid":"0000-0002-8698-3823"},{"first_name":"Peter M.","full_name":"Matthews, Peter M.","last_name":"Matthews"},{"first_name":"Ondrej","last_name":"Cais","full_name":"Cais, Ondrej"},{"first_name":"Ingo H.","full_name":"Greger, Ingo H.","last_name":"Greger"}],"title":"Gating and modulation of a hetero-octameric AMPA glutamate receptor","_id":"9549","status":"public","acknowledgement":"We thank members of the Greger laboratory, B. Herguedas, J. Krieger and J.-N. Dohrke for comments on the manuscript; J. Krieger and J.-N. Dohrke for discussion, J. Krieger for help with the normal mode analysis, B. Köhegyi for help with cryo-EM imaging, V. Chang and K. Suzuki for helping to generate the CNIH2-1D4-HA stable cell line, M. Carvalho for assistance at early stages of this project, the LMB scientific computing and the cryo-EM facility for support, P. Emsley for help with model building, T. Nakane for helpful comments with RELION 3.1 and R. Warshamanage for helping with EMDA cryo-EM-map processing. We acknowledge the Diamond Light Source for access and support of the Cryo-EM facilities at the UK national electron bio10 imaging centre (eBIC), proposal EM17434, funded by the Wellcome Trust, MRC and BBSRC. This work was supported by grants from the Medical Research Council, as part of United Kingdom Research and Innovation (also known as UK Research and Innovation) (MC_U105174197) and BBSRC (BB/N002113/1) to I.H.G.","publication_status":"published","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]}},{"day":"01","citation":{"ama":"Zhou H, Xie T, Ghazaryan A, et al. Half and quarter metals in rhombohedral trilayer graphene. <i>Nature</i>. 2021. doi:<a href=\"https://doi.org/10.1038/s41586-021-03938-w\">10.1038/s41586-021-03938-w</a>","ista":"Zhou H, Xie T, Ghazaryan A, Holder T, Ehrets JR, Spanton EM, Taniguchi T, Watanabe K, Berg E, Serbyn M, Young AF. 2021. Half and quarter metals in rhombohedral trilayer graphene. Nature.","short":"H. Zhou, T. Xie, A. Ghazaryan, T. Holder, J.R. Ehrets, E.M. Spanton, T. Taniguchi, K. Watanabe, E. Berg, M. Serbyn, A.F. Young, Nature (2021).","apa":"Zhou, H., Xie, T., Ghazaryan, A., Holder, T., Ehrets, J. R., Spanton, E. M., … Young, A. F. (2021). Half and quarter metals in rhombohedral trilayer graphene. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-021-03938-w\">https://doi.org/10.1038/s41586-021-03938-w</a>","ieee":"H. Zhou <i>et al.</i>, “Half and quarter metals in rhombohedral trilayer graphene,” <i>Nature</i>. Springer Nature, 2021.","chicago":"Zhou, Haoxin, Tian Xie, Areg Ghazaryan, Tobias Holder, James R. Ehrets, Eric M. Spanton, Takashi Taniguchi, et al. “Half and Quarter Metals in Rhombohedral Trilayer Graphene.” <i>Nature</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41586-021-03938-w\">https://doi.org/10.1038/s41586-021-03938-w</a>.","mla":"Zhou, Haoxin, et al. “Half and Quarter Metals in Rhombohedral Trilayer Graphene.” <i>Nature</i>, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41586-021-03938-w\">10.1038/s41586-021-03938-w</a>."},"month":"09","oa_version":"Preprint","arxiv":1,"article_processing_charge":"No","author":[{"last_name":"Zhou","full_name":"Zhou, Haoxin","first_name":"Haoxin"},{"last_name":"Xie","full_name":"Xie, Tian","first_name":"Tian"},{"orcid":"0000-0001-9666-3543","id":"4AF46FD6-F248-11E8-B48F-1D18A9856A87","first_name":"Areg","full_name":"Ghazaryan, Areg","last_name":"Ghazaryan"},{"full_name":"Holder, Tobias","last_name":"Holder","first_name":"Tobias"},{"full_name":"Ehrets, James R.","last_name":"Ehrets","first_name":"James R."},{"first_name":"Eric M.","full_name":"Spanton, Eric M.","last_name":"Spanton"},{"last_name":"Taniguchi","full_name":"Taniguchi, Takashi","first_name":"Takashi"},{"first_name":"Kenji","last_name":"Watanabe","full_name":"Watanabe, Kenji"},{"first_name":"Erez","full_name":"Berg, Erez","last_name":"Berg"},{"orcid":"0000-0002-2399-5827","id":"47809E7E-F248-11E8-B48F-1D18A9856A87","first_name":"Maksym","full_name":"Serbyn, Maksym","last_name":"Serbyn"},{"full_name":"Young, Andrea F.","last_name":"Young","first_name":"Andrea F."}],"title":"Half and quarter metals in rhombohedral trilayer graphene","_id":"10025","abstract":[{"lang":"eng","text":"Ferromagnetism is most common in transition metal compounds but may also arise in low-density two-dimensional electron systems, with signatures observed in silicon, III-V semiconductor systems, and graphene moiré heterostructures. Here we show that gate-tuned van Hove singularities in rhombohedral trilayer graphene drive the spontaneous ferromagnetic polarization of the electron system into one or more spin- and valley flavors. Using capacitance measurements on graphite-gated van der Waals heterostructures, we find a cascade of density- and electronic displacement field tuned phase transitions marked by negative electronic compressibility. The transitions define the boundaries between phases where quantum oscillations have either four-fold, two-fold, or one-fold degeneracy, associated with a spin and valley degenerate normal metal, spin-polarized `half-metal', and spin and valley polarized `quarter metal', respectively. For electron doping, the salient features are well captured by a phenomenological Stoner model with a valley-anisotropic Hund's coupling, likely arising from interactions at the lattice scale. For hole filling, we observe a richer phase diagram featuring a delicate interplay of broken symmetries and transitions in the Fermi surface topology. Finally, by rotational alignment of a hexagonal boron nitride substrate to induce a moiré superlattice, we find that the superlattice perturbs the preexisting isospin order only weakly, leaving the basic phase diagram intact while catalyzing the formation of topologically nontrivial gapped states whenever itinerant half- or quarter metal states occur at half- or quarter superlattice band filling. Our results show that rhombohedral trilayer graphene is an ideal platform for well-controlled tests of many-body theory and reveal magnetism in moiré materials to be fundamentally itinerant in nature."}],"doi":"10.1038/s41586-021-03938-w","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"project":[{"call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411"}],"status":"public","publication_status":"published","acknowledgement":"The authors acknowledge discussions with A. Macdonald, L. Fu, F. Wang and M. Zaletel. AFY acknowledges support of the National Science Foundation under DMR1654186, and the Gordon and Betty Moore Foundation under award GBMF9471. The authors acknowledge the use of the research facilities within the California NanoSystems Institute, supported by the University of California, Santa Barbara and the University of California, Office of the President.\r\nK.W. and T.T. acknowledge support from the Elemental Strategy Initiative conducted by the MEXT, Japan, Grant Number JPMXP0112101001 and JSPS KAKENHI, Grant Number JP20H00354. EB and TH were supported by the European Research Council (ERC) under grant HQMAT (Grant Agreement No. 817799). A.G. acknowledges support by the European Unions Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement\r\nNo. 754411.\r\n","ec_funded":1,"department":[{"_id":"MaSe"},{"_id":"MiLe"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_published":"2021-09-01T00:00:00Z","external_id":{"isi":["000706977400002"],"arxiv":["2104.00653"]},"scopus_import":"1","date_created":"2021-09-19T22:01:25Z","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/2104.00653"}],"publisher":"Springer Nature","isi":1,"type":"journal_article","language":[{"iso":"eng"}],"quality_controlled":"1","oa":1,"year":"2021","keyword":["condensed matter - mesoscale and nanoscale physics","condensed matter - strongly correlated electrons","multidisciplinary"],"publication":"Nature","date_updated":"2023-08-14T07:04:06Z","related_material":{"link":[{"url":"https://doi.org/10.1038/s41586-021-04181-z","relation":"erratum"}]},"article_type":"original"},{"year":"2021","publication":"Nature","date_updated":"2023-08-14T08:01:21Z","related_material":{"link":[{"url":"https://ist.ac.at/en/news/boosting-the-cells-power-house/","description":"News on IST Webpage","relation":"press_release"}]},"page":"364-367","article_type":"original","department":[{"_id":"LeSa"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","external_id":{"pmid":["34616041"],"isi":["000704581600001"]},"date_published":"2021-10-14T00:00:00Z","date_created":"2021-10-17T22:01:17Z","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"EM-Fac"},{"_id":"ScienComp"}],"scopus_import":"1","publisher":"Springer Nature","intvolume":"       598","isi":1,"quality_controlled":"1","type":"journal_article","language":[{"iso":"eng"}],"title":"Structure and assembly of the mammalian mitochondrial supercomplex CIII<sub>2</sub>CIV","author":[{"orcid":"0000-0001-5618-3449","full_name":"Vercellino, Irene","last_name":"Vercellino","id":"3ED6AF16-F248-11E8-B48F-1D18A9856A87","first_name":"Irene"},{"orcid":"0000-0002-0977-7989","full_name":"Sazanov, Leonid A","last_name":"Sazanov","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","first_name":"Leonid A"}],"_id":"10146","abstract":[{"lang":"eng","text":"The enzymes of the mitochondrial electron transport chain are key players of cell metabolism. Despite being active when isolated, in vivo they associate into supercomplexes1, whose precise role is debated. Supercomplexes CIII2CIV1-2 (refs. 2,3), CICIII2 (ref. 4) and CICIII2CIV (respirasome)5,6,7,8,9,10 exist in mammals, but in contrast to CICIII2 and the respirasome, to date the only known eukaryotic structures of CIII2CIV1-2 come from Saccharomyces cerevisiae11,12 and plants13, which have different organization. Here we present the first, to our knowledge, structures of mammalian (mouse and ovine) CIII2CIV and its assembly intermediates, in different conformations. We describe the assembly of CIII2CIV from the CIII2 precursor to the final CIII2CIV conformation, driven by the insertion of the N terminus of the assembly factor SCAF1 (ref. 14) deep into CIII2, while its C terminus is integrated into CIV. Our structures (which include CICIII2 and the respirasome) also confirm that SCAF1 is exclusively required for the assembly of CIII2CIV and has no role in the assembly of the respirasome. We show that CIII2 is asymmetric due to the presence of only one copy of subunit 9, which straddles both monomers and prevents the attachment of a second copy of SCAF1 to CIII2, explaining the presence of one copy of CIV in CIII2CIV in mammals. Finally, we show that CIII2 and CIV gain catalytic advantage when assembled into the supercomplex and propose a role for CIII2CIV in fine tuning the efficiency of electron transfer in the electron transport chain."}],"issue":"7880","doi":"10.1038/s41586-021-03927-z","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"project":[{"call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411"}],"publication_status":"published","acknowledgement":"We thank the pre-clinical facility of the IST Austria and A. Venturino for assistance with the animals; and V.-V. Hodirnau for assistance during the Titan Krios data collection, performed at the IST Austria. The data processing was performed at the IST high-performance computing cluster. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 754411.","status":"public","ec_funded":1,"citation":{"apa":"Vercellino, I., &#38; Sazanov, L. A. (2021). Structure and assembly of the mammalian mitochondrial supercomplex CIII<sub>2</sub>CIV. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-021-03927-z\">https://doi.org/10.1038/s41586-021-03927-z</a>","ieee":"I. Vercellino and L. A. Sazanov, “Structure and assembly of the mammalian mitochondrial supercomplex CIII<sub>2</sub>CIV,” <i>Nature</i>, vol. 598, no. 7880. Springer Nature, pp. 364–367, 2021.","ama":"Vercellino I, Sazanov LA. Structure and assembly of the mammalian mitochondrial supercomplex CIII<sub>2</sub>CIV. <i>Nature</i>. 2021;598(7880):364-367. doi:<a href=\"https://doi.org/10.1038/s41586-021-03927-z\">10.1038/s41586-021-03927-z</a>","short":"I. Vercellino, L.A. Sazanov, Nature 598 (2021) 364–367.","ista":"Vercellino I, Sazanov LA. 2021. Structure and assembly of the mammalian mitochondrial supercomplex CIII<sub>2</sub>CIV. Nature. 598(7880), 364–367.","mla":"Vercellino, Irene, and Leonid A. Sazanov. “Structure and Assembly of the Mammalian Mitochondrial Supercomplex CIII<sub>2</sub>CIV.” <i>Nature</i>, vol. 598, no. 7880, Springer Nature, 2021, pp. 364–67, doi:<a href=\"https://doi.org/10.1038/s41586-021-03927-z\">10.1038/s41586-021-03927-z</a>.","chicago":"Vercellino, Irene, and Leonid A Sazanov. “Structure and Assembly of the Mammalian Mitochondrial Supercomplex CIII<sub>2</sub>CIV.” <i>Nature</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41586-021-03927-z\">https://doi.org/10.1038/s41586-021-03927-z</a>."},"day":"14","pmid":1,"month":"10","oa_version":"None","article_processing_charge":"No","volume":598},{"status":"public","publication_status":"published","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"abstract":[{"text":"From rock salt to nanoparticle superlattices, complex structure can emerge from simple building blocks that attract each other through Coulombic forces1-4. On the micrometre scale, however, colloids in water defy the intuitively simple idea of forming crystals from oppositely charged partners, instead forming non-equilibrium structures such as clusters and gels5-7. Although various systems have been engineered to grow binary crystals8-11, native surface charge in aqueous conditions has not been used to assemble crystalline materials. Here we form ionic colloidal crystals in water through an approach that we refer to as polymer-attenuated Coulombic self-assembly. The key to crystallization is the use of a neutral polymer to keep particles separated by well defined distances, allowing us to tune the attractive overlap of electrical double layers, directing particles to disperse, crystallize or become permanently fixed on demand. The nucleation and growth of macroscopic single crystals is demonstrated by using the Debye screening length to fine-tune assembly. Using a variety of colloidal particles and commercial polymers, ionic colloidal crystals isostructural to caesium chloride, sodium chloride, aluminium diboride and K4C60 are selected according to particle size ratios. Once fixed by simply diluting out solution salts, crystals are pulled out of the water for further manipulation, demonstrating an accurate translation from solution-phase assembly to dried solid structures. In contrast to other assembly approaches, in which particles must be carefully engineered to encode binding information12-18, polymer-attenuated Coulombic self-assembly enables conventional colloids to be used as model colloidal ions, primed for crystallization. ","lang":"eng"}],"issue":"7804","doi":"10.1038/s41586-020-2205-0","title":"Ionic solids from common colloids","author":[{"full_name":"Hueckel, Theodore","last_name":"Hueckel","first_name":"Theodore"},{"first_name":"Glen M.","last_name":"Hocky","full_name":"Hocky, Glen M."},{"orcid":"0000-0002-7253-9465","full_name":"Palacci, Jérémie A","last_name":"Palacci","id":"8fb92548-2b22-11eb-b7c1-a3f0d08d7c7d","first_name":"Jérémie A"},{"first_name":"Stefano","full_name":"Sacanna, Stefano","last_name":"Sacanna"}],"_id":"9059","volume":580,"article_processing_charge":"No","oa_version":"None","citation":{"chicago":"Hueckel, Theodore, Glen M. Hocky, Jérémie A Palacci, and Stefano Sacanna. “Ionic Solids from Common Colloids.” <i>Nature</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41586-020-2205-0\">https://doi.org/10.1038/s41586-020-2205-0</a>.","mla":"Hueckel, Theodore, et al. “Ionic Solids from Common Colloids.” <i>Nature</i>, vol. 580, no. 7804, Springer Nature, 2020, pp. 487–90, doi:<a href=\"https://doi.org/10.1038/s41586-020-2205-0\">10.1038/s41586-020-2205-0</a>.","apa":"Hueckel, T., Hocky, G. M., Palacci, J. A., &#38; Sacanna, S. (2020). Ionic solids from common colloids. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-020-2205-0\">https://doi.org/10.1038/s41586-020-2205-0</a>","ieee":"T. Hueckel, G. M. Hocky, J. A. Palacci, and S. Sacanna, “Ionic solids from common colloids,” <i>Nature</i>, vol. 580, no. 7804. Springer Nature, pp. 487–490, 2020.","ista":"Hueckel T, Hocky GM, Palacci JA, Sacanna S. 2020. Ionic solids from common colloids. Nature. 580(7804), 487–490.","ama":"Hueckel T, Hocky GM, Palacci JA, Sacanna S. Ionic solids from common colloids. <i>Nature</i>. 2020;580(7804):487-490. doi:<a href=\"https://doi.org/10.1038/s41586-020-2205-0\">10.1038/s41586-020-2205-0</a>","short":"T. Hueckel, G.M. Hocky, J.A. Palacci, S. Sacanna, Nature 580 (2020) 487–490."},"day":"23","month":"04","pmid":1,"page":"487-490","article_type":"original","publication":"Nature","date_updated":"2023-02-23T13:47:55Z","year":"2020","keyword":["Multidisciplinary"],"type":"journal_article","language":[{"iso":"eng"}],"quality_controlled":"1","publisher":"Springer Nature","intvolume":"       580","extern":"1","external_id":{"pmid":["32322078"]},"date_published":"2020-04-23T00:00:00Z","date_created":"2021-02-02T13:30:50Z","scopus_import":"1","user_id":"D865714E-FA4E-11E9-B85B-F5C5E5697425"},{"citation":{"short":"B. Cheng, G. Mazzola, C.J. Pickard, M. Ceriotti, Nature 585 (2020) 217–220.","ista":"Cheng B, Mazzola G, Pickard CJ, Ceriotti M. 2020. Evidence for supercritical behaviour of high-pressure liquid hydrogen. Nature. 585(7824), 217–220.","ama":"Cheng B, Mazzola G, Pickard CJ, Ceriotti M. Evidence for supercritical behaviour of high-pressure liquid hydrogen. <i>Nature</i>. 2020;585(7824):217-220. doi:<a href=\"https://doi.org/10.1038/s41586-020-2677-y\">10.1038/s41586-020-2677-y</a>","ieee":"B. Cheng, G. Mazzola, C. J. Pickard, and M. Ceriotti, “Evidence for supercritical behaviour of high-pressure liquid hydrogen,” <i>Nature</i>, vol. 585, no. 7824. Springer Nature, pp. 217–220, 2020.","apa":"Cheng, B., Mazzola, G., Pickard, C. J., &#38; Ceriotti, M. (2020). Evidence for supercritical behaviour of high-pressure liquid hydrogen. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-020-2677-y\">https://doi.org/10.1038/s41586-020-2677-y</a>","chicago":"Cheng, Bingqing, Guglielmo Mazzola, Chris J. Pickard, and Michele Ceriotti. “Evidence for Supercritical Behaviour of High-Pressure Liquid Hydrogen.” <i>Nature</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41586-020-2677-y\">https://doi.org/10.1038/s41586-020-2677-y</a>.","mla":"Cheng, Bingqing, et al. “Evidence for Supercritical Behaviour of High-Pressure Liquid Hydrogen.” <i>Nature</i>, vol. 585, no. 7824, Springer Nature, 2020, pp. 217–20, doi:<a href=\"https://doi.org/10.1038/s41586-020-2677-y\">10.1038/s41586-020-2677-y</a>."},"day":"10","month":"09","pmid":1,"oa_version":"Preprint","arxiv":1,"volume":585,"article_processing_charge":"No","title":"Evidence for supercritical behaviour of high-pressure liquid hydrogen","author":[{"orcid":"0000-0002-3584-9632","first_name":"Bingqing","id":"cbe3cda4-d82c-11eb-8dc7-8ff94289fcc9","last_name":"Cheng","full_name":"Cheng, Bingqing"},{"first_name":"Guglielmo","last_name":"Mazzola","full_name":"Mazzola, Guglielmo"},{"full_name":"Pickard, Chris J.","last_name":"Pickard","first_name":"Chris J."},{"first_name":"Michele","last_name":"Ceriotti","full_name":"Ceriotti, Michele"}],"_id":"9685","abstract":[{"text":"Hydrogen, the simplest and most abundant element in the Universe, develops a remarkably complex behaviour upon compression^1. Since Wigner predicted the dissociation and metallization of solid hydrogen at megabar pressures almost a century ago^2, several efforts have been made to explain the many unusual properties of dense hydrogen, including a rich and poorly understood solid polymorphism^1,3-5, an anomalous melting line6 and the possible transition to a superconducting state^7. Experiments at such extreme conditions are challenging and often lead to hard-to-interpret and controversial observations, whereas theoretical investigations are constrained by the huge computational cost of sufficiently accurate quantum mechanical calculations. Here we present a theoretical study of the phase diagram of dense hydrogen that uses machine learning to 'learn' potential-energy surfaces and interatomic forces from reference calculations and then predict them at low computational cost, overcoming length- and timescale limitations. We reproduce both the re-entrant melting behaviour and the polymorphism of the solid phase. Simulations using our machine-learning-based potentials provide evidence for a continuous molecular-to-atomic transition in the liquid, with no first-order transition observed above the melting line. This suggests a smooth transition between insulating and metallic layers in giant gas planets, and reconciles existing discrepancies between experiments as a manifestation of supercritical behaviour.","lang":"eng"}],"issue":"7824","doi":"10.1038/s41586-020-2677-y","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"status":"public","publication_status":"published","user_id":"6785fbc1-c503-11eb-8a32-93094b40e1cf","date_published":"2020-09-10T00:00:00Z","extern":"1","external_id":{"arxiv":["1906.03341"],"pmid":["32908269"]},"date_created":"2021-07-19T09:17:49Z","scopus_import":"1","publisher":"Springer Nature","intvolume":"       585","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1906.03341"}],"language":[{"iso":"eng"}],"quality_controlled":"1","type":"journal_article","oa":1,"year":"2020","publication":"Nature","date_updated":"2021-08-09T12:38:01Z","page":"217-220","article_type":"original"},{"issue":"7836","abstract":[{"lang":"eng","text":"Magnetism typically arises from the joint effect of Fermi statistics and repulsive Coulomb interactions, which favours ground states with non-zero electron spin. As a result, controlling spin magnetism with electric fields—a longstanding technological goal in spintronics and multiferroics1,2—can be achieved only indirectly. Here we experimentally demonstrate direct electric-field control of magnetic states in an orbital Chern insulator3,4,5,6, a magnetic system in which non-trivial band topology favours long-range order of orbital angular momentum but the spins are thought to remain disordered7,8,9,10,11,12,13,14. We use van der Waals heterostructures consisting of a graphene monolayer rotationally faulted with respect to a Bernal-stacked bilayer to realize narrow and topologically non-trivial valley-projected moiré minibands15,16,17. At fillings of one and three electrons per moiré unit cell within these bands, we observe quantized anomalous Hall effects18 with transverse resistance approximately equal to h/2e2 (where h is Planck’s constant and e is the charge on the electron), which is indicative of spontaneous polarization of the system into a single-valley-projected band with a Chern number equal to two. At a filling of three electrons per moiré unit cell, we find that the sign of the quantum anomalous Hall effect can be reversed via field-effect control of the chemical potential; moreover, this transition is hysteretic, which we use to demonstrate non-volatile electric-field-induced reversal of the magnetic state. A theoretical analysis19 indicates that the effect arises from the topological edge states, which drive a change in sign of the magnetization and thus a reversal in the favoured magnetic state. Voltage control of magnetic states can be used to electrically pattern non-volatile magnetic-domain structures hosting chiral edge states, with applications ranging from reconfigurable microwave circuit elements to ultralow-power magnetic memories."}],"doi":"10.1038/s41586-020-2963-8","author":[{"last_name":"Polshyn","full_name":"Polshyn, Hryhoriy","first_name":"Hryhoriy","id":"edfc7cb1-526e-11ec-b05a-e6ecc27e4e48","orcid":"0000-0001-8223-8896"},{"first_name":"J.","last_name":"Zhu","full_name":"Zhu, J."},{"first_name":"M. A.","last_name":"Kumar","full_name":"Kumar, M. A."},{"first_name":"Y.","last_name":"Zhang","full_name":"Zhang, Y."},{"last_name":"Yang","full_name":"Yang, F.","first_name":"F."},{"first_name":"C. L.","full_name":"Tschirhart, C. L.","last_name":"Tschirhart"},{"first_name":"M.","last_name":"Serlin","full_name":"Serlin, M."},{"first_name":"K.","last_name":"Watanabe","full_name":"Watanabe, K."},{"first_name":"T.","full_name":"Taniguchi, T.","last_name":"Taniguchi"},{"first_name":"A. H.","last_name":"MacDonald","full_name":"MacDonald, A. H."},{"first_name":"A. F.","last_name":"Young","full_name":"Young, A. F."}],"title":"Electrical switching of magnetic order in an orbital Chern insulator","_id":"10618","publication_status":"published","status":"public","acknowledgement":"We acknowledge discussions with J. Checkelsky, S. Chen, C. Dean, M. Yankowitz, D. Reilly, I. Sodemann and M. Zaletel. Work at UCSB was primarily supported by the ARO under MURI W911NF-16-1-0361. Measurements of twisted bilayer graphene (Extended Data Fig. 8) and measurements at elevated temperatures (Extended Data Fig. 3) were supported by a SEED grant and made use of shared facilities of the UCSB MRSEC (NSF DMR 1720256), a member of the Materials Research Facilities Network (www.mrfn.org). A.F.Y. acknowledges the support of the David and Lucille Packard Foundation under award 2016-65145. A.H.M. and J.Z. were supported by the National Science Foundation through the Center for Dynamics and Control of Materials, an NSF MRSEC under Cooperative Agreement number DMR-1720595, and by the Welch Foundation under grant TBF1473. C.L.T. acknowledges support from the Hertz Foundation and from the National Science Foundation Graduate Research Fellowship Program under grant 1650114. K.W. and T.T. acknowledge support from the Elemental Strategy Initiative conducted by the MEXT, Japan, Grant Number JPMXP0112101001, JSPS KAKENHI grant numbers JP20H00354 and the CREST(JPMJCR15F3), JST.","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"oa_version":"Preprint","day":"23","citation":{"mla":"Polshyn, Hryhoriy, et al. “Electrical Switching of Magnetic Order in an Orbital Chern Insulator.” <i>Nature</i>, vol. 588, no. 7836, Springer Nature, 2020, pp. 66–70, doi:<a href=\"https://doi.org/10.1038/s41586-020-2963-8\">10.1038/s41586-020-2963-8</a>.","chicago":"Polshyn, Hryhoriy, J. Zhu, M. A. Kumar, Y. Zhang, F. Yang, C. L. Tschirhart, M. Serlin, et al. “Electrical Switching of Magnetic Order in an Orbital Chern Insulator.” <i>Nature</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41586-020-2963-8\">https://doi.org/10.1038/s41586-020-2963-8</a>.","apa":"Polshyn, H., Zhu, J., Kumar, M. A., Zhang, Y., Yang, F., Tschirhart, C. L., … Young, A. F. (2020). Electrical switching of magnetic order in an orbital Chern insulator. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-020-2963-8\">https://doi.org/10.1038/s41586-020-2963-8</a>","ieee":"H. Polshyn <i>et al.</i>, “Electrical switching of magnetic order in an orbital Chern insulator,” <i>Nature</i>, vol. 588, no. 7836. Springer Nature, pp. 66–70, 2020.","ista":"Polshyn H, Zhu J, Kumar MA, Zhang Y, Yang F, Tschirhart CL, Serlin M, Watanabe K, Taniguchi T, MacDonald AH, Young AF. 2020. Electrical switching of magnetic order in an orbital Chern insulator. Nature. 588(7836), 66–70.","short":"H. Polshyn, J. Zhu, M.A. Kumar, Y. Zhang, F. Yang, C.L. Tschirhart, M. Serlin, K. Watanabe, T. Taniguchi, A.H. MacDonald, A.F. Young, Nature 588 (2020) 66–70.","ama":"Polshyn H, Zhu J, Kumar MA, et al. Electrical switching of magnetic order in an orbital Chern insulator. <i>Nature</i>. 2020;588(7836):66-70. doi:<a href=\"https://doi.org/10.1038/s41586-020-2963-8\">10.1038/s41586-020-2963-8</a>"},"month":"11","pmid":1,"volume":588,"article_processing_charge":"No","arxiv":1,"year":"2020","keyword":["multidisciplinary"],"oa":1,"article_type":"original","page":"66-70","publication":"Nature","date_updated":"2022-01-13T14:21:04Z","date_published":"2020-11-23T00:00:00Z","external_id":{"arxiv":["2004.11353"],"pmid":["33230333"]},"extern":"1","scopus_import":"1","date_created":"2022-01-13T14:12:17Z","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","type":"journal_article","language":[{"iso":"eng"}],"quality_controlled":"1","intvolume":"       588","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/2004.11353"}],"publisher":"Springer Nature"},{"publisher":"Springer Nature","intvolume":"       577","volume":577,"article_processing_charge":"No","quality_controlled":"1","language":[{"iso":"eng"}],"type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"01","citation":{"mla":"Immerzeel, W. W., et al. “Importance and Vulnerability of the World’s Water Towers.” <i>Nature</i>, vol. 577, no. 7790, Springer Nature, 2020, pp. 364–69, doi:<a href=\"https://doi.org/10.1038/s41586-019-1822-y\">10.1038/s41586-019-1822-y</a>.","chicago":"Immerzeel, W. W., A. F. Lutz, M. Andrade, A. Bahl, H. Biemans, T. Bolch, S. Hyde, et al. “Importance and Vulnerability of the World’s Water Towers.” <i>Nature</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41586-019-1822-y\">https://doi.org/10.1038/s41586-019-1822-y</a>.","apa":"Immerzeel, W. W., Lutz, A. F., Andrade, M., Bahl, A., Biemans, H., Bolch, T., … Baillie, J. E. M. (2020). Importance and vulnerability of the world’s water towers. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-019-1822-y\">https://doi.org/10.1038/s41586-019-1822-y</a>","ieee":"W. W. Immerzeel <i>et al.</i>, “Importance and vulnerability of the world’s water towers,” <i>Nature</i>, vol. 577, no. 7790. Springer Nature, pp. 364–369, 2020.","ista":"Immerzeel WW, Lutz AF, Andrade M, Bahl A, Biemans H, Bolch T, Hyde S, Brumby S, Davies BJ, Elmore AC, Emmer A, Feng M, Fernández A, Haritashya U, Kargel JS, Koppes M, Kraaijenbrink PDA, Kulkarni AV, Mayewski PA, Nepal S, Pacheco P, Painter TH, Pellicciotti F, Rajaram H, Rupper S, Sinisalo A, Shrestha AB, Viviroli D, Wada Y, Xiao C, Yao T, Baillie JEM. 2020. Importance and vulnerability of the world’s water towers. Nature. 577(7790), 364–369.","short":"W.W. Immerzeel, A.F. Lutz, M. Andrade, A. Bahl, H. Biemans, T. Bolch, S. Hyde, S. Brumby, B.J. Davies, A.C. Elmore, A. Emmer, M. Feng, A. Fernández, U. Haritashya, J.S. Kargel, M. Koppes, P.D.A. Kraaijenbrink, A.V. Kulkarni, P.A. Mayewski, S. Nepal, P. Pacheco, T.H. Painter, F. Pellicciotti, H. Rajaram, S. Rupper, A. Sinisalo, A.B. Shrestha, D. Viviroli, Y. Wada, C. Xiao, T. Yao, J.E.M. Baillie, Nature 577 (2020) 364–369.","ama":"Immerzeel WW, Lutz AF, Andrade M, et al. Importance and vulnerability of the world’s water towers. <i>Nature</i>. 2020;577(7790):364-369. doi:<a href=\"https://doi.org/10.1038/s41586-019-1822-y\">10.1038/s41586-019-1822-y</a>"},"day":"16","oa_version":"None","date_created":"2023-02-20T08:12:53Z","scopus_import":"1","extern":"1","date_published":"2020-01-16T00:00:00Z","date_updated":"2023-02-28T12:17:38Z","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"publication":"Nature","page":"364-369","article_type":"original","status":"public","publication_status":"published","_id":"12599","title":"Importance and vulnerability of the world’s water towers","author":[{"first_name":"W. W.","full_name":"Immerzeel, W. W.","last_name":"Immerzeel"},{"full_name":"Lutz, A. F.","last_name":"Lutz","first_name":"A. F."},{"first_name":"M.","full_name":"Andrade, M.","last_name":"Andrade"},{"last_name":"Bahl","full_name":"Bahl, A.","first_name":"A."},{"full_name":"Biemans, H.","last_name":"Biemans","first_name":"H."},{"last_name":"Bolch","full_name":"Bolch, T.","first_name":"T."},{"last_name":"Hyde","full_name":"Hyde, S.","first_name":"S."},{"last_name":"Brumby","full_name":"Brumby, S.","first_name":"S."},{"first_name":"B. J.","last_name":"Davies","full_name":"Davies, B. J."},{"full_name":"Elmore, A. C.","last_name":"Elmore","first_name":"A. C."},{"first_name":"A.","full_name":"Emmer, A.","last_name":"Emmer"},{"last_name":"Feng","full_name":"Feng, M.","first_name":"M."},{"first_name":"A.","full_name":"Fernández, A.","last_name":"Fernández"},{"last_name":"Haritashya","full_name":"Haritashya, U.","first_name":"U."},{"last_name":"Kargel","full_name":"Kargel, J. S.","first_name":"J. S."},{"first_name":"M.","full_name":"Koppes, M.","last_name":"Koppes"},{"first_name":"P. D. A.","last_name":"Kraaijenbrink","full_name":"Kraaijenbrink, P. D. A."},{"last_name":"Kulkarni","full_name":"Kulkarni, A. V.","first_name":"A. V."},{"full_name":"Mayewski, P. A.","last_name":"Mayewski","first_name":"P. A."},{"first_name":"S.","last_name":"Nepal","full_name":"Nepal, S."},{"full_name":"Pacheco, P.","last_name":"Pacheco","first_name":"P."},{"full_name":"Painter, T. H.","last_name":"Painter","first_name":"T. H."},{"last_name":"Pellicciotti","full_name":"Pellicciotti, Francesca","first_name":"Francesca","id":"b28f055a-81ea-11ed-b70c-a9fe7f7b0e70"},{"first_name":"H.","last_name":"Rajaram","full_name":"Rajaram, H."},{"last_name":"Rupper","full_name":"Rupper, S.","first_name":"S."},{"first_name":"A.","full_name":"Sinisalo, A.","last_name":"Sinisalo"},{"last_name":"Shrestha","full_name":"Shrestha, A. B.","first_name":"A. B."},{"full_name":"Viviroli, D.","last_name":"Viviroli","first_name":"D."},{"first_name":"Y.","full_name":"Wada, Y.","last_name":"Wada"},{"first_name":"C.","full_name":"Xiao, C.","last_name":"Xiao"},{"first_name":"T.","full_name":"Yao, T.","last_name":"Yao"},{"full_name":"Baillie, J. E. M.","last_name":"Baillie","first_name":"J. E. M."}],"doi":"10.1038/s41586-019-1822-y","abstract":[{"text":"Mountains are the water towers of the world, supplying a substantial part of both natural and anthropogenic water demands1,2. They are highly sensitive and prone to climate change3,4, yet their importance and vulnerability have not been quantified at the global scale. Here we present a global water tower index (WTI), which ranks all water towers in terms of their water-supplying role and the downstream dependence of ecosystems and society. For each water tower, we assess its vulnerability related to water stress, governance, hydropolitical tension and future climatic and socio-economic changes. We conclude that the most important (highest WTI) water towers are also among the most vulnerable, and that climatic and socio-economic changes will affect them profoundly. This could negatively impact 1.9 billion people living in (0.3 billion) or directly downstream of (1.6 billion) mountainous areas. Immediate action is required to safeguard the future of the world’s most important and vulnerable water towers.","lang":"eng"}],"issue":"7790","year":"2020"}]