---
_id: '6470'
abstract:
- lang: eng
  text: 'Investigating neuronal activity using genetically encoded Ca2+ indicators
    in behaving animals is hampered by inaccuracies in spike inference from fluorescent
    tracers. Here we combine two‐photon [Ca2+] imaging with cell‐attached recordings,
    followed by post hoc determination of the expression level of GCaMP6f, to explore
    how it affects the amplitude, kinetics and temporal summation of somatic [Ca2+]
    transients in mouse hippocampal pyramidal cells (PCs). The amplitude of unitary
    [Ca2+] transients (evoked by a single action potential) negatively correlates
    with GCaMP6f expression, but displays large variability even among PCs with similarly
    low expression levels. The summation of fluorescence signals is frequency‐dependent,
    supralinear and also shows remarkable cell‐to‐cell variability. We performed experimental
    data‐based simulations and found that spike inference error rates using MLspike
    depend strongly on unitary peak amplitudes and GCaMP6f expression levels. We provide
    simple methods for estimating the unitary [Ca2+] transients in individual weakly
    GCaMP6f‐expressing PCs, with which we achieve spike inference error rates of ∼5%. '
article_processing_charge: No
article_type: original
author:
- first_name: Tímea
  full_name: Éltes, Tímea
  last_name: Éltes
- first_name: Miklos
  full_name: Szoboszlay, Miklos
  last_name: Szoboszlay
- first_name: Margit Katalin
  full_name: Szigeti, Margit Katalin
  id: 44F4BDC0-F248-11E8-B48F-1D18A9856A87
  last_name: Szigeti
  orcid: 0000-0001-9500-8758
- first_name: Zoltan
  full_name: Nusser, Zoltan
  last_name: Nusser
citation:
  ama: Éltes T, Szoboszlay M, Szigeti MK, Nusser Z. Improved spike inference accuracy
    by estimating the peak amplitude of unitary [Ca2+] transients in weakly GCaMP6f-expressing
    hippocampal pyramidal cells. <i>Journal of Physiology</i>. 2019;597(11):2925–2947.
    doi:<a href="https://doi.org/10.1113/JP277681">10.1113/JP277681</a>
  apa: Éltes, T., Szoboszlay, M., Szigeti, M. K., &#38; Nusser, Z. (2019). Improved
    spike inference accuracy by estimating the peak amplitude of unitary [Ca2+] transients
    in weakly GCaMP6f-expressing hippocampal pyramidal cells. <i>Journal of Physiology</i>.
    Wiley. <a href="https://doi.org/10.1113/JP277681">https://doi.org/10.1113/JP277681</a>
  chicago: Éltes, Tímea, Miklos Szoboszlay, Margit Katalin Szigeti, and Zoltan Nusser.
    “Improved Spike Inference Accuracy by Estimating the Peak Amplitude of Unitary
    [Ca2+] Transients in Weakly GCaMP6f-Expressing Hippocampal Pyramidal Cells.” <i>Journal
    of Physiology</i>. Wiley, 2019. <a href="https://doi.org/10.1113/JP277681">https://doi.org/10.1113/JP277681</a>.
  ieee: T. Éltes, M. Szoboszlay, M. K. Szigeti, and Z. Nusser, “Improved spike inference
    accuracy by estimating the peak amplitude of unitary [Ca2+] transients in weakly
    GCaMP6f-expressing hippocampal pyramidal cells,” <i>Journal of Physiology</i>,
    vol. 597, no. 11. Wiley, pp. 2925–2947, 2019.
  ista: Éltes T, Szoboszlay M, Szigeti MK, Nusser Z. 2019. Improved spike inference
    accuracy by estimating the peak amplitude of unitary [Ca2+] transients in weakly
    GCaMP6f-expressing hippocampal pyramidal cells. Journal of Physiology. 597(11),
    2925–2947.
  mla: Éltes, Tímea, et al. “Improved Spike Inference Accuracy by Estimating the Peak
    Amplitude of Unitary [Ca2+] Transients in Weakly GCaMP6f-Expressing Hippocampal
    Pyramidal Cells.” <i>Journal of Physiology</i>, vol. 597, no. 11, Wiley, 2019,
    pp. 2925–2947, doi:<a href="https://doi.org/10.1113/JP277681">10.1113/JP277681</a>.
  short: T. Éltes, M. Szoboszlay, M.K. Szigeti, Z. Nusser, Journal of Physiology 597
    (2019) 2925–2947.
date_created: 2019-05-19T21:59:17Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2023-08-25T10:34:15Z
day: '01'
department:
- _id: GaNo
doi: 10.1113/JP277681
external_id:
  isi:
  - '000470780400013'
  pmid:
  - '31006863'
intvolume: '       597'
isi: 1
issue: '11'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1113/JP277681
month: '06'
oa: 1
oa_version: Published Version
page: 2925–2947
pmid: 1
publication: Journal of Physiology
publication_identifier:
  eissn:
  - '14697793'
  issn:
  - '00223751'
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Improved spike inference accuracy by estimating the peak amplitude of unitary
  [Ca2+] transients in weakly GCaMP6f-expressing hippocampal pyramidal cells
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 597
year: '2019'
...