[{"title":"In vivo analysis of uropod function during physiological T cell trafficking","citation":{"short":"S. Soriano, M. Hons, K. Schumann, V. Kumar, T. Dennier, R. Lyck, M.K. Sixt, J. Stein, Journal of Immunology 187 (2011) 2356–2364.","apa":"Soriano, S., Hons, M., Schumann, K., Kumar, V., Dennier, T., Lyck, R., … Stein, J. (2011). In vivo analysis of uropod function during physiological T cell trafficking. <i>Journal of Immunology</i>. American Association of Immunologists. <a href=\"https://doi.org/10.4049/jimmunol.1100935\">https://doi.org/10.4049/jimmunol.1100935</a>","ama":"Soriano S, Hons M, Schumann K, et al. In vivo analysis of uropod function during physiological T cell trafficking. <i>Journal of Immunology</i>. 2011;187(5):2356-2364. doi:<a href=\"https://doi.org/10.4049/jimmunol.1100935\">10.4049/jimmunol.1100935</a>","ieee":"S. Soriano <i>et al.</i>, “In vivo analysis of uropod function during physiological T cell trafficking,” <i>Journal of Immunology</i>, vol. 187, no. 5. American Association of Immunologists, pp. 2356–2364, 2011.","ista":"Soriano S, Hons M, Schumann K, Kumar V, Dennier T, Lyck R, Sixt MK, Stein J. 2011. In vivo analysis of uropod function during physiological T cell trafficking. Journal of Immunology. 187(5), 2356–2364.","chicago":"Soriano, Silvia, Miroslav Hons, Kathrin Schumann, Varsha Kumar, Timo Dennier, Ruth Lyck, Michael K Sixt, and Jens Stein. “In Vivo Analysis of Uropod Function during Physiological T Cell Trafficking.” <i>Journal of Immunology</i>. American Association of Immunologists, 2011. <a href=\"https://doi.org/10.4049/jimmunol.1100935\">https://doi.org/10.4049/jimmunol.1100935</a>.","mla":"Soriano, Silvia, et al. “In Vivo Analysis of Uropod Function during Physiological T Cell Trafficking.” <i>Journal of Immunology</i>, vol. 187, no. 5, American Association of Immunologists, 2011, pp. 2356–64, doi:<a href=\"https://doi.org/10.4049/jimmunol.1100935\">10.4049/jimmunol.1100935</a>."},"type":"journal_article","author":[{"last_name":"Soriano","full_name":"Soriano, Silvia","first_name":"Silvia"},{"orcid":"0000-0002-6625-3348","last_name":"Hons","first_name":"Miroslav","full_name":"Hons, Miroslav"},{"full_name":"Schumann, Kathrin","first_name":"Kathrin","last_name":"Schumann"},{"full_name":"Kumar, Varsha","first_name":"Varsha","last_name":"Kumar"},{"full_name":"Dennier, Timo","first_name":"Timo","last_name":"Dennier"},{"first_name":"Ruth","full_name":"Lyck, Ruth","last_name":"Lyck"},{"last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Stein, Jens","first_name":"Jens","last_name":"Stein"}],"day":"01","doi":"10.4049/jimmunol.1100935","language":[{"iso":"eng"}],"page":"2356 - 2364","date_created":"2018-12-11T12:03:04Z","month":"09","publisher":"American Association of Immunologists","status":"public","intvolume":"       187","department":[{"_id":"MiSi"}],"quality_controlled":"1","publication":"Journal of Immunology","oa_version":"None","year":"2011","publist_id":"3215","article_type":"original","publication_identifier":{"eissn":["1550-6606"],"issn":["0022-1767"]},"date_updated":"2023-10-10T13:14:59Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","issue":"5","article_processing_charge":"No","abstract":[{"lang":"eng","text":"Migrating lymphocytes acquire a polarized phenotype with a leading and a trailing edge, or uropod. Although in vitro experiments in cell lines or activated primary cell cultures have established that Rho-p160 coiled-coil kinase (ROCK)-myosin II-mediated uropod contractility is required for integrin de-adhesion on two-dimensional surfaces and nuclear propulsion through narrow pores in three-dimensional matrices, less is known about the role of these two events during the recirculation of primary, nonactivated lymphocytes. Using pharmacological antagonists of ROCK and myosin II, we report that inhibition of uropod contractility blocked integrin-independent mouse T cell migration through narrow, but not large, pores in vitro. T cell crawling on chemokine-coated endothelial cells under shear was severely impaired by ROCK inhibition, whereas transendothelial migration was only reduced through endothelial cells with high, but not low, barrier properties. Using three-dimensional thick-tissue imaging and dynamic two-photon microscopy of T cell motility in lymphoid tissue, we demonstrated a significant role for uropod contractility in intraluminal crawling and transendothelial migration through lymph node, but not bone marrow, endothelial cells. Finally, we demonstrated that ICAM-1, but not anatomical constraints or integrin-independent interactions, reduced parenchymal motility of inhibitor-treated T cells within the dense lymphoid microenvironment, thus assigning context-dependent roles for uropod contraction during lymphocyte recirculation."}],"_id":"3392","date_published":"2011-09-01T00:00:00Z","publication_status":"published","volume":187},{"date_updated":"2023-05-11T13:37:29Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","external_id":{"pmid":["11145713"]},"publication_identifier":{"issn":["0022-1767"]},"publist_id":"2200","article_type":"original","oa_version":"None","year":"2001","publication_status":"published","oa":1,"main_file_link":[{"open_access":"1","url":"http://www.jimmunol.org/content/166/2/1300.long"}],"volume":166,"issue":"2","article_processing_charge":"No","_id":"3927","date_published":"2001-01-15T00:00:00Z","abstract":[{"text":"TNF-alpha has been clearly identified as central mediator of T cell activation-induced acute hepatic injury in mice, e.g., Con A hepatitis. In this model, liver injury depends on both TNFRs, i.e., the 55-kDa TNFR1 as well as the 75-kDa TNFR2. We show in this report that the hepatic TNFRs are not transcriptionally regulated, but are regulated by receptor shedding. TNF directly mediates hepatocellular death by activation of TNFR1 but also induces the expression of inflammatory proteins, such as cytokines and adhesion molecules. Here we provide evidence that resistance of TNFR1(-/-) and TNFR2(-/-) mice against Con A hepatitis is not due to an impaired production of the central mediators TNF and IFN-gamma. Con A injection results in a massive induction of ICAM-1, VCAM-1, and E-selectin in the liver. Lack of either one of both TNFRs did not change adhesion molecule expression in the livers of Con A-treated mice, presumably reflecting the fact that other endothelial cell-activating cytokines up-regulated adhesion molecule expression. However, treatment of TNFR1(-/-) and TNFR2(-/-) mice with murine rTNF revealed a predominant role for TNFR1 for the induction of hepatic adhesion molecule expression. Pretreatment with blocking Abs against E- and P-selectin or of ICAM(-/-) mice with anti-VCAM-1 Abs failed to prevent Con A hepatitis, although accumulation of the critical cell population, i.e., CD4(+) T cells was significantly inhibited. Hence, up-regulation of adhesion molecules during acute hepatitis unlikely contributes to organ injury but rather represents a defense mechanism.","lang":"eng"}],"acknowledgement":"We thank Dr. H. Bluethmann (F. Hoffmann-LaRoche AG, Basle, Switzerland) for kindly providing us TNFR knockout mice. We are indebted to Dr. G. R. Adolf (Bender & Co Vienna, Austria) for providing recombinant murine TNF. We are also indebted to Dr. D. Vestweber for providing anti-P-selectin mAb (23). We thank Dr. W. Neuhuber (Institute of  Anatomy, University of Erlangen-NÜrnberg, Erlangen, Germany) for experimental support regarding confocal laser scanning microscopy. The perfect technical assistance of Andrea Agli is gratefully acknowledged.","pmid":1,"language":[{"iso":"eng"}],"doi":"10.4049/jimmunol.166.2.1300","citation":{"mla":"Wolf, Dominik, et al. “TNF-α-Induced Expression of Adhesion Molecules in the Liver Is under the Control of TNFR1--Relevance for Concanavalin A-Induced Hepatitis.” <i>Journal of Immunology</i>, vol. 166, no. 2, American Association of Immunologists, 2001, pp. 1300–07, doi:<a href=\"https://doi.org/10.4049/jimmunol.166.2.1300\">10.4049/jimmunol.166.2.1300</a>.","ieee":"D. Wolf <i>et al.</i>, “TNF-α-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis,” <i>Journal of Immunology</i>, vol. 166, no. 2. American Association of Immunologists, pp. 1300–1307, 2001.","ista":"Wolf D, Hallmann R, Sass G, Sixt MK, Küsters S, Fregien B, Trautwein C, Tiegs G. 2001. TNF-α-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis. Journal of Immunology. 166(2), 1300–1307.","chicago":"Wolf, Dominik, Rupert Hallmann, Gabriele Sass, Michael K Sixt, Sabine Küsters, Bastian Fregien, Christian Trautwein, and Gisa Tiegs. “TNF-α-Induced Expression of Adhesion Molecules in the Liver Is under the Control of TNFR1--Relevance for Concanavalin A-Induced Hepatitis.” <i>Journal of Immunology</i>. American Association of Immunologists, 2001. <a href=\"https://doi.org/10.4049/jimmunol.166.2.1300\">https://doi.org/10.4049/jimmunol.166.2.1300</a>.","apa":"Wolf, D., Hallmann, R., Sass, G., Sixt, M. K., Küsters, S., Fregien, B., … Tiegs, G. (2001). TNF-α-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis. <i>Journal of Immunology</i>. American Association of Immunologists. <a href=\"https://doi.org/10.4049/jimmunol.166.2.1300\">https://doi.org/10.4049/jimmunol.166.2.1300</a>","ama":"Wolf D, Hallmann R, Sass G, et al. TNF-α-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis. <i>Journal of Immunology</i>. 2001;166(2):1300-1307. doi:<a href=\"https://doi.org/10.4049/jimmunol.166.2.1300\">10.4049/jimmunol.166.2.1300</a>","short":"D. Wolf, R. Hallmann, G. Sass, M.K. Sixt, S. Küsters, B. Fregien, C. Trautwein, G. Tiegs, Journal of Immunology 166 (2001) 1300–1307."},"title":"TNF-α-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis","day":"15","author":[{"full_name":"Wolf, Dominik","first_name":"Dominik","last_name":"Wolf"},{"full_name":"Hallmann, Rupert","first_name":"Rupert","last_name":"Hallmann"},{"last_name":"Sass","first_name":"Gabriele","full_name":"Sass, Gabriele"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Küsters, Sabine","first_name":"Sabine","last_name":"Küsters"},{"full_name":"Fregien, Bastian","first_name":"Bastian","last_name":"Fregien"},{"first_name":"Christian","full_name":"Trautwein, Christian","last_name":"Trautwein"},{"first_name":"Gisa","full_name":"Tiegs, Gisa","last_name":"Tiegs"}],"type":"journal_article","publisher":"American Association of Immunologists","quality_controlled":"1","publication":"Journal of Immunology","status":"public","intvolume":"       166","page":"1300 - 1307","extern":"1","month":"01","date_created":"2018-12-11T12:05:56Z"}]