---
_id: '5676'
abstract:
- lang: eng
  text: 'In epithelial tissues, cells tightly connect to each other through cell–cell
    junctions, but they also present the remarkable capacity of reorganizing themselves
    without compromising tissue integrity. Upon injury, simple epithelia efficiently
    resolve small lesions through the action of actin cytoskeleton contractile structures
    at the wound edge and cellular rearrangements. However, the underlying mechanisms
    and how they cooperate are still poorly understood. In this study, we combine
    live imaging and theoretical modeling to reveal a novel and indispensable role
    for occluding junctions (OJs) in this process. We demonstrate that OJ loss of
    function leads to defects in wound-closure dynamics: instead of contracting, wounds
    dramatically increase their area. OJ mutants exhibit phenotypes in cell shape,
    cellular rearrangements, and mechanical properties as well as in actin cytoskeleton
    dynamics at the wound edge. We propose that OJs are essential for wound closure
    by impacting on epithelial mechanics at the tissue level, which in turn is crucial
    for correct regulation of the cellular events occurring at the wound edge.'
article_processing_charge: No
author:
- first_name: Lara
  full_name: Carvalho, Lara
  last_name: Carvalho
- first_name: Pedro
  full_name: Patricio, Pedro
  last_name: Patricio
- first_name: Susana
  full_name: Ponte, Susana
  last_name: Ponte
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Luis
  full_name: Almeida, Luis
  last_name: Almeida
- first_name: André S.
  full_name: Nunes, André S.
  last_name: Nunes
- first_name: Nuno A.M.
  full_name: Araújo, Nuno A.M.
  last_name: Araújo
- first_name: Antonio
  full_name: Jacinto, Antonio
  last_name: Jacinto
citation:
  ama: Carvalho L, Patricio P, Ponte S, et al. Occluding junctions as novel regulators
    of tissue mechanics during wound repair. <i>Journal of Cell Biology</i>. 2018;217(12):4267-4283.
    doi:<a href="https://doi.org/10.1083/jcb.201804048">10.1083/jcb.201804048</a>
  apa: Carvalho, L., Patricio, P., Ponte, S., Heisenberg, C.-P. J., Almeida, L., Nunes,
    A. S., … Jacinto, A. (2018). Occluding junctions as novel regulators of tissue
    mechanics during wound repair. <i>Journal of Cell Biology</i>. Rockefeller University
    Press. <a href="https://doi.org/10.1083/jcb.201804048">https://doi.org/10.1083/jcb.201804048</a>
  chicago: Carvalho, Lara, Pedro Patricio, Susana Ponte, Carl-Philipp J Heisenberg,
    Luis Almeida, André S. Nunes, Nuno A.M. Araújo, and Antonio Jacinto. “Occluding
    Junctions as Novel Regulators of Tissue Mechanics during Wound Repair.” <i>Journal
    of Cell Biology</i>. Rockefeller University Press, 2018. <a href="https://doi.org/10.1083/jcb.201804048">https://doi.org/10.1083/jcb.201804048</a>.
  ieee: L. Carvalho <i>et al.</i>, “Occluding junctions as novel regulators of tissue
    mechanics during wound repair,” <i>Journal of Cell Biology</i>, vol. 217, no.
    12. Rockefeller University Press, pp. 4267–4283, 2018.
  ista: Carvalho L, Patricio P, Ponte S, Heisenberg C-PJ, Almeida L, Nunes AS, Araújo
    NAM, Jacinto A. 2018. Occluding junctions as novel regulators of tissue mechanics
    during wound repair. Journal of Cell Biology. 217(12), 4267–4283.
  mla: Carvalho, Lara, et al. “Occluding Junctions as Novel Regulators of Tissue Mechanics
    during Wound Repair.” <i>Journal of Cell Biology</i>, vol. 217, no. 12, Rockefeller
    University Press, 2018, pp. 4267–83, doi:<a href="https://doi.org/10.1083/jcb.201804048">10.1083/jcb.201804048</a>.
  short: L. Carvalho, P. Patricio, S. Ponte, C.-P.J. Heisenberg, L. Almeida, A.S.
    Nunes, N.A.M. Araújo, A. Jacinto, Journal of Cell Biology 217 (2018) 4267–4283.
date_created: 2018-12-16T22:59:19Z
date_published: 2018-12-01T00:00:00Z
date_updated: 2023-09-13T09:11:17Z
day: '01'
department:
- _id: CaHe
doi: 10.1083/jcb.201804048
ec_funded: 1
external_id:
  isi:
  - '000451960800018'
  pmid:
  - '30228162 '
intvolume: '       217'
isi: 1
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pubmed/30228162
month: '12'
oa: 1
oa_version: Submitted Version
page: 4267-4283
pmid: 1
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
publication: Journal of Cell Biology
publication_identifier:
  issn:
  - '00219525'
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Occluding junctions as novel regulators of tissue mechanics during wound repair
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 217
year: '2018'
...