---
_id: '9955'
abstract:
- lang: eng
text: Neurons can change their classical neurotransmitters during ontogeny, sometimes
going through stages of dual release. Here, we explored the development of the
neurotransmitter identity of neurons of the avian nucleus isthmi parvocellularis
(Ipc), whose axon terminals are retinotopically arranged in the optic tectum (TeO)
and exert a focal gating effect upon the ascending transmission of retinal inputs.
Although cholinergic and glutamatergic markers are both found in Ipc neurons and
terminals of adult pigeons and chicks, the mRNA expression of the vesicular acetylcholine
transporter, VAChT, is weak or absent. To explore how the Ipc neurotransmitter
identity is established during ontogeny, we analyzed the expression of mRNAs coding
for cholinergic (ChAT, VAChT, and CHT) and glutamatergic (VGluT2 and VGluT3) markers
in chick embryos at different developmental stages. We found that between E12
and E18, Ipc neurons expressed all cholinergic mRNAs and also VGluT2 mRNA; however,
from E16 through posthatch stages, VAChT mRNA expression was specifically diminished.
Our ex vivo deposits of tracer crystals and intracellular filling experiments
revealed that Ipc axons exhibit a mature paintbrush morphology late in development,
experiencing marked morphological transformations during the period of presumptive
dual vesicular transmitter release. Additionally, although ChAT protein immunoassays
increasingly label the growing Ipc axon, this labeling was consistently restricted
to sparse portions of the terminal branches. Combined, these results suggest that
the synthesis of glutamate and acetylcholine, and their vesicular release, is
complexly linked to the developmental processes of branching, growing and remodeling
of these unique axons.
acknowledgement: 'This work was supported by FONDECYT grants 1151432 and 1210169 to
Gonzalo J. Marín. FONDECYT grant 1210069 to Jorge Mpodozis. Spanish Ministry of
Science, Innovation and Universities (MCIU), State Research Agency (AEI) and European
Regional Development Fund (FEDER), PGC2018-098229-B-100 to José L Ferrán. Spanish
Ministry of Economy and Competitiveness Excellency Grant BFU2014-57516P (with European
Community FEDER support), and a Seneca Foundation (Autonomous Community of Murcia)
Excellency Research contract, ref: 19904/ GERM/15; project name: Genoarchitectonic
Brain Development and Applications to Neurodegenerative Diseases and Cancer (5672
Fundación Séneca) to Luis Puelles. The authors gratefully acknowledge the valuable
editorial help provided by Sara Fernández-Collemann. The authors also thank Elisa
Sentis and Solano Henríquez for expert technical help.'
article_processing_charge: No
article_type: original
author:
- first_name: Rosana
full_name: Reyes‐Pinto, Rosana
last_name: Reyes‐Pinto
- first_name: José L.
full_name: Ferrán, José L.
last_name: Ferrán
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Cristian
full_name: González‐Cabrera, Cristian
last_name: González‐Cabrera
- first_name: Harald
full_name: Luksch, Harald
last_name: Luksch
- first_name: Jorge
full_name: Mpodozis, Jorge
last_name: Mpodozis
- first_name: Luis
full_name: Puelles, Luis
last_name: Puelles
- first_name: Gonzalo J.
full_name: Marín, Gonzalo J.
last_name: Marín
citation:
ama: Reyes‐Pinto R, Ferrán JL, Vega Zuniga TA, et al. Change in the neurochemical
signature and morphological development of the parvocellular isthmic projection
to the avian tectum. Journal of Comparative Neurology. 2022;530(2):553-573.
doi:10.1002/cne.25229
apa: Reyes‐Pinto, R., Ferrán, J. L., Vega Zuniga, T. A., González‐Cabrera, C., Luksch,
H., Mpodozis, J., … Marín, G. J. (2022). Change in the neurochemical signature
and morphological development of the parvocellular isthmic projection to the avian
tectum. Journal of Comparative Neurology. Wiley. https://doi.org/10.1002/cne.25229
chicago: Reyes‐Pinto, Rosana, José L. Ferrán, Tomas A Vega Zuniga, Cristian González‐Cabrera,
Harald Luksch, Jorge Mpodozis, Luis Puelles, and Gonzalo J. Marín. “Change in
the Neurochemical Signature and Morphological Development of the Parvocellular
Isthmic Projection to the Avian Tectum.” Journal of Comparative Neurology.
Wiley, 2022. https://doi.org/10.1002/cne.25229.
ieee: R. Reyes‐Pinto et al., “Change in the neurochemical signature and morphological
development of the parvocellular isthmic projection to the avian tectum,” Journal
of Comparative Neurology, vol. 530, no. 2. Wiley, pp. 553–573, 2022.
ista: Reyes‐Pinto R, Ferrán JL, Vega Zuniga TA, González‐Cabrera C, Luksch H, Mpodozis
J, Puelles L, Marín GJ. 2022. Change in the neurochemical signature and morphological
development of the parvocellular isthmic projection to the avian tectum. Journal
of Comparative Neurology. 530(2), 553–573.
mla: Reyes‐Pinto, Rosana, et al. “Change in the Neurochemical Signature and Morphological
Development of the Parvocellular Isthmic Projection to the Avian Tectum.” Journal
of Comparative Neurology, vol. 530, no. 2, Wiley, 2022, pp. 553–73, doi:10.1002/cne.25229.
short: R. Reyes‐Pinto, J.L. Ferrán, T.A. Vega Zuniga, C. González‐Cabrera, H. Luksch,
J. Mpodozis, L. Puelles, G.J. Marín, Journal of Comparative Neurology 530 (2022)
553–573.
date_created: 2021-08-23T08:40:59Z
date_published: 2022-02-01T00:00:00Z
date_updated: 2023-08-11T10:58:17Z
day: '01'
department:
- _id: MaJö
doi: 10.1002/cne.25229
external_id:
isi:
- '000686420000001'
pmid:
- '34363623'
intvolume: ' 530'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 553-573
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
eissn:
- 1096-9861
issn:
- 0021-9967
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Change in the neurochemical signature and morphological development of the
parvocellular isthmic projection to the avian tectum
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 530
year: '2022'
...
---
_id: '7148'
abstract:
- lang: eng
text: In the cerebellum, GluD2 is exclusively expressed in Purkinje cells, where
it regulates synapse formation and regeneration, synaptic plasticity, and motor
learning. Delayed cognitive development in humans with GluD2 gene mutations suggests
extracerebellar functions of GluD2. However, extracerebellar expression of GluD2
and its relationship with that of GluD1 are poorly understood. GluD2 mRNA and
protein were widely detected, with relatively high levels observed in the olfactory
glomerular layer, medial prefrontal cortex, cingulate cortex, retrosplenial granular
cortex, olfactory tubercle, subiculum, striatum, lateral septum, anterodorsal
thalamic nucleus, and arcuate hypothalamic nucleus. These regions were also enriched
for GluD1, and many individual neurons coexpressed the two GluDs. In the retrosplenial
granular cortex, GluD1 and GluD2 were selectively expressed at PSD‐95‐expressing
glutamatergic synapses, and their coexpression on the same synapses was shown
by SDS‐digested freeze‐fracture replica labeling. Biochemically, GluD1 and GluD2
formed coimmunoprecipitable complex formation in HEK293T cells and in the cerebral
cortex and hippocampus. We further estimated the relative protein amount by quantitative
immunoblotting using GluA2/GluD2 and GluA2/GluD1 chimeric proteins as standards
for titration of GluD1 and GluD2 antibodies. Intriguingly, the relative amount
of GluD2 was almost comparable to that of GluD1 in the postsynaptic density fraction
prepared from the cerebral cortex and hippocampus. In contrast, GluD2 was overwhelmingly
predominant in the cerebellum. Thus, we have determined the relative extracerebellar
expression of GluD1 and GluD2 at regional, neuronal, and synaptic levels. These
data provide a molecular–anatomical basis for possible competitive and cooperative
interactions of GluD family members at synapses in various brain regions.
acknowledgement: This study was supported by Grants-in-Aid for Scientific Research
to K.K. (18K06813), Y.M. (17K08503, 17H0631319), and K.S. (16H04650) and a grant
for Scientific Research on Innovative Areas to K.S (16H06276) from the Ministry
of Education, Culture, Sports, Science and Technology of Japan (MEXT). We thank
K. Akashi, I. Watanabe-Iida, Y. Suzuki, and H. Azechi for technical assistance and
advice, and H. Uchida for valuable discussions. We thank E. Kushiya,I. Yabe, C.
Ohori, Y. Mochizuki, Y. Ishikawa, and N. Ishimoto for technical assistance in generating
GluD1-KO mice.
article_processing_charge: No
article_type: original
author:
- first_name: Chihiro
full_name: Nakamoto, Chihiro
last_name: Nakamoto
- first_name: Kohtarou
full_name: Konno, Kohtarou
last_name: Konno
- first_name: Taisuke
full_name: Miyazaki, Taisuke
last_name: Miyazaki
- first_name: Ena
full_name: Nakatsukasa, Ena
last_name: Nakatsukasa
- first_name: Rie
full_name: Natsume, Rie
last_name: Natsume
- first_name: Manabu
full_name: Abe, Manabu
last_name: Abe
- first_name: Meiko
full_name: Kawamura, Meiko
last_name: Kawamura
- first_name: Yugo
full_name: Fukazawa, Yugo
last_name: Fukazawa
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Miwako
full_name: Yamasaki, Miwako
last_name: Yamasaki
- first_name: Kenji
full_name: Sakimura, Kenji
last_name: Sakimura
- first_name: Masahiko
full_name: Watanabe, Masahiko
last_name: Watanabe
citation:
ama: Nakamoto C, Konno K, Miyazaki T, et al. Expression mapping, quantification,
and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain.
Journal of Comparative Neurology. 2020;528(6):1003-1027. doi:10.1002/cne.24792
apa: Nakamoto, C., Konno, K., Miyazaki, T., Nakatsukasa, E., Natsume, R., Abe, M.,
… Watanabe, M. (2020). Expression mapping, quantification, and complex formation
of GluD1 and GluD2 glutamate receptors in adult mouse brain. Journal of Comparative
Neurology. Wiley. https://doi.org/10.1002/cne.24792
chicago: Nakamoto, Chihiro, Kohtarou Konno, Taisuke Miyazaki, Ena Nakatsukasa, Rie
Natsume, Manabu Abe, Meiko Kawamura, et al. “Expression Mapping, Quantification,
and Complex Formation of GluD1 and GluD2 Glutamate Receptors in Adult Mouse Brain.”
Journal of Comparative Neurology. Wiley, 2020. https://doi.org/10.1002/cne.24792.
ieee: C. Nakamoto et al., “Expression mapping, quantification, and complex
formation of GluD1 and GluD2 glutamate receptors in adult mouse brain,” Journal
of Comparative Neurology, vol. 528, no. 6. Wiley, pp. 1003–1027, 2020.
ista: Nakamoto C, Konno K, Miyazaki T, Nakatsukasa E, Natsume R, Abe M, Kawamura
M, Fukazawa Y, Shigemoto R, Yamasaki M, Sakimura K, Watanabe M. 2020. Expression
mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors
in adult mouse brain. Journal of Comparative Neurology. 528(6), 1003–1027.
mla: Nakamoto, Chihiro, et al. “Expression Mapping, Quantification, and Complex
Formation of GluD1 and GluD2 Glutamate Receptors in Adult Mouse Brain.” Journal
of Comparative Neurology, vol. 528, no. 6, Wiley, 2020, pp. 1003–27, doi:10.1002/cne.24792.
short: C. Nakamoto, K. Konno, T. Miyazaki, E. Nakatsukasa, R. Natsume, M. Abe, M.
Kawamura, Y. Fukazawa, R. Shigemoto, M. Yamasaki, K. Sakimura, M. Watanabe, Journal
of Comparative Neurology 528 (2020) 1003–1027.
date_created: 2019-12-04T16:09:29Z
date_published: 2020-04-01T00:00:00Z
date_updated: 2023-08-17T14:06:50Z
day: '01'
ddc:
- '571'
- '599'
department:
- _id: RySh
doi: 10.1002/cne.24792
external_id:
isi:
- '000496410200001'
pmid:
- '31625608'
has_accepted_license: '1'
intvolume: ' 528'
isi: 1
issue: '6'
language:
- iso: eng
month: '04'
oa_version: None
page: 1003-1027
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
eissn:
- 1096-9861
issn:
- 0021-9967
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Expression mapping, quantification, and complex formation of GluD1 and GluD2
glutamate receptors in adult mouse brain
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 528
year: '2020'
...
---
_id: '2618'
abstract:
- lang: eng
text: The unipolar brush cell (UBC) is a type of glutamatergic interneuron in the
granular layer of the cerebellum. The UBC brush and a single mossy fiber (MF)
terminal contact each other within a cerebellar glomerulus, forming a giant synapse.
Many UBCs receive input from extrinsic MFs, whereas others are innervated by intrinsic
mossy terminals formed by the axons of other UBCs. In all mammalian species so
far examined, the vestibulocerebellum is enriched of UBCs that are strongly immunoreactive
for the calcium binding protein calretinin (CR) in both the somatodendritic and
axonal compartment. UBCs have postsynaptic ionotropic glutamate receptors and
extrasynaptic metabotropic glutamate receptors that immunocytochemically highlight
their somatodendritic compartment and brush, respectively. In this study on the
mouse cerebellum, we present evidence that immunoreactivities to CR and mGluR1α
define two distinct UBC subsets with partly overlapping distributions in lobule
X (the nodulus). In sections double-labeled for CR and mGluR1α, the patterns of
distributions of CR+/mGluR1α- UBCs and CR-/mGluR1α+ UBCs differed along the mediolateral
and dorsoventral axes of the folium. Moreover, mGluR1α+ UBCs outnumbered CR+ UBCs.
Both UBC subsets were mGluR2/3, GluR2/3, and NMDAR1 immunoreactive. The different
distribution patterns of the two UBC subsets within lobule X suggest that expression
of CR or mGluR1α by UBCs may be afferent-specific and related to the terminal
fields of different vestibular MF afferents.
article_processing_charge: No
article_type: original
author:
- first_name: Maria
full_name: Nunzi, Maria
last_name: Nunzi
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Enrico
full_name: Mugnaini, Enrico
last_name: Mugnaini
citation:
ama: Nunzi M, Shigemoto R, Mugnaini E. Differential expression of calretinin and
metabotropic glutamate receptor mGluR1α defines subsets of unipolar brush cells
in mouse cerebellum. Journal of Comparative Neurology. 2002;451(2):189-199.
doi:10.1002/cne.10344
apa: Nunzi, M., Shigemoto, R., & Mugnaini, E. (2002). Differential expression
of calretinin and metabotropic glutamate receptor mGluR1α defines subsets of unipolar
brush cells in mouse cerebellum. Journal of Comparative Neurology. Wiley-Blackwell.
https://doi.org/10.1002/cne.10344
chicago: Nunzi, Maria, Ryuichi Shigemoto, and Enrico Mugnaini. “Differential Expression
of Calretinin and Metabotropic Glutamate Receptor MGluR1α Defines Subsets of Unipolar
Brush Cells in Mouse Cerebellum.” Journal of Comparative Neurology. Wiley-Blackwell,
2002. https://doi.org/10.1002/cne.10344.
ieee: M. Nunzi, R. Shigemoto, and E. Mugnaini, “Differential expression of calretinin
and metabotropic glutamate receptor mGluR1α defines subsets of unipolar brush
cells in mouse cerebellum,” Journal of Comparative Neurology, vol. 451,
no. 2. Wiley-Blackwell, pp. 189–199, 2002.
ista: Nunzi M, Shigemoto R, Mugnaini E. 2002. Differential expression of calretinin
and metabotropic glutamate receptor mGluR1α defines subsets of unipolar brush
cells in mouse cerebellum. Journal of Comparative Neurology. 451(2), 189–199.
mla: Nunzi, Maria, et al. “Differential Expression of Calretinin and Metabotropic
Glutamate Receptor MGluR1α Defines Subsets of Unipolar Brush Cells in Mouse Cerebellum.”
Journal of Comparative Neurology, vol. 451, no. 2, Wiley-Blackwell, 2002,
pp. 189–99, doi:10.1002/cne.10344.
short: M. Nunzi, R. Shigemoto, E. Mugnaini, Journal of Comparative Neurology 451
(2002) 189–199.
date_created: 2018-12-11T11:58:42Z
date_published: 2002-09-16T00:00:00Z
date_updated: 2023-07-25T09:09:48Z
day: '16'
doi: 10.1002/cne.10344
extern: '1'
external_id:
pmid:
- '12209836'
intvolume: ' 451'
issue: '2'
language:
- iso: eng
month: '09'
oa_version: None
page: 189 - 199
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4279'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Differential expression of calretinin and metabotropic glutamate receptor mGluR1α
defines subsets of unipolar brush cells in mouse cerebellum
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 451
year: '2002'
...
---
_id: '2604'
abstract:
- lang: eng
text: Cutaneous antidromic vasodilatation and plasma extravasation, two phenomena
that occur in neurogenic inflammation, are partially blocked by substance P (SP)
receptor antagonists and are known to be mediated in part by mast cell-released
substances, such as histamine, serotonin, and nitric oxide. In an attempt to provide
a morphological substrate for the above phenomena, we applied light and electron
microscopic immunocytochemistry to investigate the pattern of SP innervation of
blood vessels and its relationship to mast cells in the skin of the rat lower
lip. Furthermore, we examined the distribution of SP (neurokinin-1) receptors
and their relationship to SP-immunoreactive (IR) fibers. Our results confirmed
that SP-IR fibers are found in cutaneous nerves and that terminal branches are
observed around blood vessels and penetrating the epidermis. SP-IR fibers also
innervated hair follicles and sebaceous glands. At the ultrastructural level,
SP-IR varicosities were observed adjacent to arterioles, capillaries, venules,
and mast cells. The varicosities possessed both dense core vesicles and agranular
synaptic vesicles. We quantified the distance between SP-IR varicosities and blood
vessel endothelial cells. SP-IR terminals were located within 0.23-5.99 μm from
the endothelial cell layer in 82.7% of arterioles, in 90.2% of capillaries, and
in 86.9% of venules. Although there was a trend for SP-IR fibers to be located
closer to the endothelium of venules, this difference was not significant. Neurokinin-1
receptor (NK-1r) immunoreactivity was most abundant in the upper dermis and was
associated with the wall of blood vessels. NK-1r were located in equal amounts
on the walls of arterioles, capillaries, and venules that were innervated by SP-IR
fibers. The present results favor the concept of a participation of SP in cutaneous
neurogenic vasodilatation and plasma extravasation both by an action on blood
vessels after binding to the NK-1r and by causing the release of substances from
mast cells after diffusion through the connective tissue.
acknowledgement: This work was sponsored by grant MT-12170 from the Canadian Medical
Research Council. The authors thank Marie Ballak for electron microscopy assistance,
Alan Forster for photographic expertise, and Sid Parkinson for editorial
assistance.
article_processing_charge: No
article_type: original
author:
- first_name: Isabella
full_name: Ruocco, Isabella
last_name: Ruocco
- first_name: Augusto
full_name: Cuello, Augusto
last_name: Cuello
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Alfredo
full_name: Ribeiro Da Silva, Alfredo
last_name: Ribeiro Da Silva
citation:
ama: Ruocco I, Cuello A, Shigemoto R, Ribeiro Da Silva A. Light and electron microscopic
study of the distribution of substance P-immunoreactive fibers and neurokinin-1
receptors in the skin of the rat lower lip. Journal of Comparative Neurology.
2001;432(4):466-480. doi:10.1002/cne.1114
apa: Ruocco, I., Cuello, A., Shigemoto, R., & Ribeiro Da Silva, A. (2001). Light
and electron microscopic study of the distribution of substance P-immunoreactive
fibers and neurokinin-1 receptors in the skin of the rat lower lip. Journal
of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/cne.1114
chicago: Ruocco, Isabella, Augusto Cuello, Ryuichi Shigemoto, and Alfredo Ribeiro
Da Silva. “Light and Electron Microscopic Study of the Distribution of Substance
P-Immunoreactive Fibers and Neurokinin-1 Receptors in the Skin of the Rat Lower
Lip.” Journal of Comparative Neurology. Wiley-Blackwell, 2001. https://doi.org/10.1002/cne.1114.
ieee: I. Ruocco, A. Cuello, R. Shigemoto, and A. Ribeiro Da Silva, “Light and electron
microscopic study of the distribution of substance P-immunoreactive fibers and
neurokinin-1 receptors in the skin of the rat lower lip,” Journal of Comparative
Neurology, vol. 432, no. 4. Wiley-Blackwell, pp. 466–480, 2001.
ista: Ruocco I, Cuello A, Shigemoto R, Ribeiro Da Silva A. 2001. Light and electron
microscopic study of the distribution of substance P-immunoreactive fibers and
neurokinin-1 receptors in the skin of the rat lower lip. Journal of Comparative
Neurology. 432(4), 466–480.
mla: Ruocco, Isabella, et al. “Light and Electron Microscopic Study of the Distribution
of Substance P-Immunoreactive Fibers and Neurokinin-1 Receptors in the Skin of
the Rat Lower Lip.” Journal of Comparative Neurology, vol. 432, no. 4,
Wiley-Blackwell, 2001, pp. 466–80, doi:10.1002/cne.1114.
short: I. Ruocco, A. Cuello, R. Shigemoto, A. Ribeiro Da Silva, Journal of Comparative
Neurology 432 (2001) 466–480.
date_created: 2018-12-11T11:58:37Z
date_published: 2001-04-16T00:00:00Z
date_updated: 2023-05-24T13:03:51Z
day: '16'
doi: 10.1002/cne.1114
extern: '1'
external_id:
pmid:
- '11268009'
intvolume: ' 432'
issue: '4'
language:
- iso: eng
month: '04'
oa_version: None
page: 466 - 480
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4294'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Light and electron microscopic study of the distribution of substance P-immunoreactive
fibers and neurokinin-1 receptors in the skin of the rat lower lip
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 432
year: '2001'
...
---
_id: '2599'
abstract:
- lang: eng
text: 'The synaptic relationship between substance P (SP) and its receptor, i.e.,
neurokinin-1 receptor (NK1R), was examined in the striatum of the rat by confocal
laser-scanning microscopy and electron microscopy. For confocal laser-scanning
microscopy, triple-immunofluorescence histochemistry was performed to label NK1R,
SP, and vesicular acetylcholine transporter (a specific marker for cholinergic
neurons). In electron microscopic double- immunolabeling study, immunoreactivity
for NK1R was detected with the silver- intensified gold method, while immunoreactivity
for SP was detected with peroxidase immunohistochemistry. Simultaneous immunolabeling
of NK1R and SP revealed significant mismatch at the synaptic level: although some
SP- immunopositive axon terminals were in synaptic contact with NK1R- immunopositive
sites of plasma membrane, NK1R-immunoreactivity was observed at both synaptic
and non-synaptic sites of plasma membrane. Thus, SP released from the sites remote
from NK1Rs might diffuse in the extracellular fluid to act, as a paracrine neurotransmitter,
on NK1Rs distant from its releasing site. SP neurotransmission in the striatum
might occur not only synaptically but also extrasynaptically. The SP-NK1R system
might constitute an association system within the striatum.'
acknowledgement: The authors are grateful for the photographic help of Ms. Yue-Ping Yuan, Mr. Akira Uesugi, Ms. Keiko Oka-moto,
Mr. Nobuyuki Kobayashi, and Mr. Hideki Itabashi. The authors also express their
gratitudes for the support of Dr. Kajitaro Morita in Morita Clinic of Internal Medicine
and Pediatrics, Kadoma, Osaka, Japan
article_processing_charge: No
article_type: original
author:
- first_name: Jin
full_name: Li, Jin
last_name: Li
- first_name: Dan
full_name: Wang, Dan
last_name: Wang
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Sakashi
full_name: Nomura, Sakashi
last_name: Nomura
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. Relationship between
neurokinin-1 receptor and substance P in the striatum: Light and electron microscopic
immunohistochemical study in the rat. Journal of Comparative Neurology.
2000;418(2):156-163. doi:10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z'
apa: 'Li, J., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., & Mizuno, N.
(2000). Relationship between neurokinin-1 receptor and substance P in the striatum:
Light and electron microscopic immunohistochemical study in the rat. Journal
of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z'
chicago: 'Li, Jin, Dan Wang, Takeshi Kaneko, Ryuichi Shigemoto, Sakashi Nomura,
and Noboru Mizuno. “Relationship between Neurokinin-1 Receptor and Substance P
in the Striatum: Light and Electron Microscopic Immunohistochemical Study in the
Rat.” Journal of Comparative Neurology. Wiley-Blackwell, 2000. https://doi.org/10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z.'
ieee: 'J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, and N. Mizuno, “Relationship
between neurokinin-1 receptor and substance P in the striatum: Light and electron
microscopic immunohistochemical study in the rat,” Journal of Comparative Neurology,
vol. 418, no. 2. Wiley-Blackwell, pp. 156–163, 2000.'
ista: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. 2000. Relationship
between neurokinin-1 receptor and substance P in the striatum: Light and electron
microscopic immunohistochemical study in the rat. Journal of Comparative Neurology.
418(2), 156–163.'
mla: 'Li, Jin, et al. “Relationship between Neurokinin-1 Receptor and Substance
P in the Striatum: Light and Electron Microscopic Immunohistochemical Study in
the Rat.” Journal of Comparative Neurology, vol. 418, no. 2, Wiley-Blackwell,
2000, pp. 156–63, doi:10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z.'
short: J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, N. Mizuno, Journal of
Comparative Neurology 418 (2000) 156–163.
date_created: 2018-12-11T11:58:36Z
date_published: 2000-03-06T00:00:00Z
date_updated: 2023-05-03T10:56:44Z
day: '06'
doi: 10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z
extern: '1'
external_id:
pmid:
- '10701441'
intvolume: ' 418'
issue: '2'
language:
- iso: eng
month: '03'
oa_version: None
page: 156 - 163
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4299'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Relationship between neurokinin-1 receptor and substance P in the striatum:
Light and electron microscopic immunohistochemical study in the rat'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 418
year: '2000'
...
---
_id: '2596'
abstract:
- lang: eng
text: "A γ-aminobutyric acid (GABA)(B) receptor (named GABA(B)R1) has been recently
cloned in the rat and human brain and two variants generated by alternative RNA
splicing were identified. In the present study, we addressed the question as to
whether these variants contribute to the diversity of GABA(B) receptor-mediated
physiological responses and constitute real receptor subtypes with distinct functions.
To this aim, we have mapped the GABA(B)R1 (R1a) and GABA(B)R1b (R1b) transcript
distribution in the rat brain using in situ hybridization. We have compared the
mRNA distribution with the distribution of [ 3H]CGP54626-labeled binding GABA(B)R1
receptor sites as assessed in adjacent cryosections by quantitative autoradiography.
We found that GABA(B) receptor transcripts and binding sites are expressed in
the brain in almost all neuronal cell populations. Expression in glial cells,
if any, is marginal. We observed a good parallelism between GABA(B)R1 mRNA transcripts
and binding sites in broad neuroanatomical entities with highest densities in
hippocampus, thalamic nuclei, and cerebellum. By contrast, R1a and R1b transcripts
exhibit marked differences in their regional and cellular distribution pattern.
A typical example is the cerebellum with an almost exclusive expression of R1b
in the Purkinje cells and of R1a in the granule, stellate, and basket cells. Data
pointing at a pre- versus postsynaptic localization for R1a and R1b, respectively,
at some neuronal sites are presented.\r\n"
acknowledgement: We thank W. Froestl and S.J. Mickel for synthesis of GABAB ligands,
and H. van der Putten for helpful discussions. Many thanks to S. Pfister (former
graduate student in our group), and P. Dreja, M. Kohler, P. Schwarb, P.Kaindl (Carl
Zeiss A.G., Zurich, Switzerland) for their help in the design of computer macros
for the quantitative analysis of in situ hybridization sections. The critical review
of the manuscript by J. Mosbacher (NovartisPharma, TA Nervous System) and J. Koenig
(Maryland Psychiatric Research Center, Baltimore, MD) are gratefully acknowledged.
article_processing_charge: No
article_type: original
author:
- first_name: Serge
full_name: Bischoff, Serge
last_name: Bischoff
- first_name: Sabine
full_name: Leonhard, Sabine
last_name: Leonhard
- first_name: Nicole
full_name: Reymann, Nicole
last_name: Reymann
- first_name: Valérie
full_name: Schuler, Valérie
last_name: Schuler
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Klemens
full_name: Kaupmann, Klemens
last_name: Kaupmann
- first_name: Bernhard
full_name: Bettler, Bernhard
last_name: Bettler
citation:
ama: Bischoff S, Leonhard S, Reymann N, et al. Spatial distribution of GABA(B)R1
receptor mRNA and binding sites in the rat brain^. Journal of Comparative Neurology.
1999;412(1):1-16. doi:10.1002/(SICI)1096-9861(19990913)412:1<1::AID-CNE1>3.0.CO;2-D
apa: Bischoff, S., Leonhard, S., Reymann, N., Schuler, V., Shigemoto, R., Kaupmann,
K., & Bettler, B. (1999). Spatial distribution of GABA(B)R1 receptor mRNA
and binding sites in the rat brain^. Journal of Comparative Neurology.
Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19990913)412:1<1::AID-CNE1>3.0.CO;2-D
chicago: Bischoff, Serge, Sabine Leonhard, Nicole Reymann, Valérie Schuler, Ryuichi
Shigemoto, Klemens Kaupmann, and Bernhard Bettler. “Spatial Distribution of GABA(B)R1
Receptor MRNA and Binding Sites in the Rat Brain^.” Journal of Comparative
Neurology. Wiley-Blackwell, 1999. https://doi.org/10.1002/(SICI)1096-9861(19990913)412:1<1::AID-CNE1>3.0.CO;2-D.
ieee: S. Bischoff et al., “Spatial distribution of GABA(B)R1 receptor mRNA
and binding sites in the rat brain^,” Journal of Comparative Neurology,
vol. 412, no. 1. Wiley-Blackwell, pp. 1–16, 1999.
ista: Bischoff S, Leonhard S, Reymann N, Schuler V, Shigemoto R, Kaupmann K, Bettler
B. 1999. Spatial distribution of GABA(B)R1 receptor mRNA and binding sites in
the rat brain^. Journal of Comparative Neurology. 412(1), 1–16.
mla: Bischoff, Serge, et al. “Spatial Distribution of GABA(B)R1 Receptor MRNA and
Binding Sites in the Rat Brain^.” Journal of Comparative Neurology, vol.
412, no. 1, Wiley-Blackwell, 1999, pp. 1–16, doi:10.1002/(SICI)1096-9861(19990913)412:1<1::AID-CNE1>3.0.CO;2-D.
short: S. Bischoff, S. Leonhard, N. Reymann, V. Schuler, R. Shigemoto, K. Kaupmann,
B. Bettler, Journal of Comparative Neurology 412 (1999) 1–16.
date_created: 2018-12-11T11:58:35Z
date_published: 1999-09-13T00:00:00Z
date_updated: 2022-09-13T08:30:25Z
day: '13'
doi: 10.1002/(SICI)1096-9861(19990913)412:1<1::AID-CNE1>3.0.CO;2-D
extern: '1'
external_id:
pmid:
- '10440706 '
intvolume: ' 412'
issue: '1'
language:
- iso: eng
month: '09'
oa_version: None
page: 1 - 16
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4303'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Spatial distribution of GABA(B)R1 receptor mRNA and binding sites in the rat
brain^
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 412
year: '1999'
...
---
_id: '2584'
abstract:
- lang: eng
text: The distributions of two alternative splicing variants of metabotropic glutamate
receptor mGluR7, mGluR7a and mGluR7b, were examined immunohistochemically in the
rat and mouse by using variant-specific antibodies raised against C-terminal portions
of rat mGluR7a and human mGluR7b. Many regions throughout the central nervous
system (CNS) showed mGluR7-like immunoreactivities (LI). The distribution patterns
of mGluR7-LI in the rat were substantially the same as those in the mouse, although
some species differences were observed in a few regions. Intense mGluR7a-LI was
seen in the main and accessory olfactory bulbs, anterior olfactory nucleus, islands
of Calleja, superficial layers of the olfactory tubercle, piriform cortex and
entorhinal cortex, periamygdaloid cortex, amygdalohippocampal area, hippocampus,
layer I of the neocortical regions, globus pallidus, superficial layers of the
superior colliculus, locus coeruleus, and superficial layers of the medullary
and spinal dorsal horns. The distribution of mGluR7b was more restricted. It was
intense in the islands of Calleja, substantia innominata, hippocampus, ventral
pallidum, and globus pallidus. The medial habenular nucleus also showed intense
mGluR7a-LI in the rat but not in the mouse. For both mGluR7a- and mGluR7b-LI,
localization in the active zones of presynaptic axon terminals was confirmed electron
microscopically at synapses of both the asymmetrical and symmetrical types. It
is noteworthy that mGluR7a-LI is seen preferentially in relay nuclei of the sensory
pathways and that both mGluR7a- and mGluR7b-LI are observed not only in presumed
glutamatergic axon terminals, but also in non-glutamatergic axon terminals including
presumed inhibitory ones. Thus, mGluR7 may play roles not only as an autoreceptor
in glutamatergic axon terminals, but also as a presynaptic heteroreceptor in non-glutamatergic
axon terminals in various CNS regions.
acknowledgement: The authors are grateful for photographic help of Mr.Akira
Uesugi, and the support of Dr. Kajitaro Morita inMorita Clinic of Internal Medicine
and Pediatrics, Kadoma,Osaka, Japan. The authors also express gratitude for thesupport of Dr. Satoru Fukuchi, Dr. Ritsu Hayashi, Dr.Sohzaburo Hayashi, Dr. Mizuho Katsurada, Dr. HitoshiKawai,
Dr. Yutaka Kitani, Dr. Toshihiko Kuroda, Dr. KeikoKumagai, Dr. Hiroshi Matsubara, Dr. Hiroshi Mat-sushima, Dr. Chisato Minakuchi, Dr. Gonpei Niwa, Dr.Hajime
Oda, Dr. Mashiko Ohbayashi, Dr. Sei-ichi Ohbaya-shi, Dr. Hiroyasu Ohtsuka, Dr. Shigeo
Tamaki, Dr. EizoWatanabe, Dr. Kazuo Yoshino, and Dr. Toshiaki Yoshino.
article_processing_charge: No
article_type: original
author:
- first_name: Ayae
full_name: Kinoshita, Ayae
last_name: Kinoshita
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Herman
full_name: Van Der Putten, Herman
last_name: Van Der Putten
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Kinoshita A, Shigemoto R, Ohishi H, Van Der Putten H, Mizuno N. Immunohistochemical
localization of metabotropic glutamate receptors, mGluR7a and mGluR7b, in the
central nervous system of the adult rat and mouse: A light and electron microscopic
study. Journal of Comparative Neurology. 1998;393(3):332-352. doi:10.1002/(SICI)1096-9861(19980413)393:3<332::AID-CNE6>3.0.CO;2-2'
apa: 'Kinoshita, A., Shigemoto, R., Ohishi, H., Van Der Putten, H., & Mizuno,
N. (1998). Immunohistochemical localization of metabotropic glutamate receptors,
mGluR7a and mGluR7b, in the central nervous system of the adult rat and mouse:
A light and electron microscopic study. Journal of Comparative Neurology.
Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19980413)393:3<332::AID-CNE6>3.0.CO;2-2'
chicago: 'Kinoshita, Ayae, Ryuichi Shigemoto, Hitoshi Ohishi, Herman Van Der Putten,
and Noboru Mizuno. “Immunohistochemical Localization of Metabotropic Glutamate
Receptors, MGluR7a and MGluR7b, in the Central Nervous System of the Adult Rat
and Mouse: A Light and Electron Microscopic Study.” Journal of Comparative
Neurology. Wiley-Blackwell, 1998. https://doi.org/10.1002/(SICI)1096-9861(19980413)393:3<332::AID-CNE6>3.0.CO;2-2.'
ieee: 'A. Kinoshita, R. Shigemoto, H. Ohishi, H. Van Der Putten, and N. Mizuno,
“Immunohistochemical localization of metabotropic glutamate receptors, mGluR7a
and mGluR7b, in the central nervous system of the adult rat and mouse: A light
and electron microscopic study,” Journal of Comparative Neurology, vol.
393, no. 3. Wiley-Blackwell, pp. 332–352, 1998.'
ista: 'Kinoshita A, Shigemoto R, Ohishi H, Van Der Putten H, Mizuno N. 1998. Immunohistochemical
localization of metabotropic glutamate receptors, mGluR7a and mGluR7b, in the
central nervous system of the adult rat and mouse: A light and electron microscopic
study. Journal of Comparative Neurology. 393(3), 332–352.'
mla: 'Kinoshita, Ayae, et al. “Immunohistochemical Localization of Metabotropic
Glutamate Receptors, MGluR7a and MGluR7b, in the Central Nervous System of the
Adult Rat and Mouse: A Light and Electron Microscopic Study.” Journal of Comparative
Neurology, vol. 393, no. 3, Wiley-Blackwell, 1998, pp. 332–52, doi:10.1002/(SICI)1096-9861(19980413)393:3<332::AID-CNE6>3.0.CO;2-2.'
short: A. Kinoshita, R. Shigemoto, H. Ohishi, H. Van Der Putten, N. Mizuno, Journal
of Comparative Neurology 393 (1998) 332–352.
date_created: 2018-12-11T11:58:31Z
date_published: 1998-03-13T00:00:00Z
date_updated: 2022-09-01T12:11:04Z
day: '13'
doi: 10.1002/(SICI)1096-9861(19980413)393:3<332::AID-CNE6>3.0.CO;2-2
extern: '1'
external_id:
pmid:
- '9548554'
intvolume: ' 393'
issue: '3'
language:
- iso: eng
month: '03'
oa_version: None
page: 332 - 352
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4314'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Immunohistochemical localization of metabotropic glutamate receptors, mGluR7a
and mGluR7b, in the central nervous system of the adult rat and mouse: A light and
electron microscopic study'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 393
year: '1998'
...
---
_id: '2585'
abstract:
- lang: eng
text: Localization of metabetropic glutamate receptor subtypes, mGluR1, mGluRlu,
mGluR2/3, mGluR4a, mGluR5, mGluR7a, mGluR7b, and mGluR8, was examined in some
of the target areas of projection fibers from the main and accessory olfactory
bulbs (MOB and AOB) by using subtype-specific antibodies. The superficial layer
of the olfactory tubercle and layer Ia of the pitiform cortex, the target areas
of MOB, showed marked mGluR1-, mGluR5-, mGluR7a-, and mGluR8-like immunoreactivities
(-LI), and rather weak mGluR2/3-LI. The periamygdaloid cortical region including
the target areas of both MOB and AOB showed intense mGluR2/3-LI as well as marked
mGluRl-, mGluR5-, mGluR7a-, and mGluRS-LI. No significant mGluR1a-, mGluR4a-,
or mGluR7b-LI was seen in these regions. After transection of the lateral olfactory
tract, mGluR2/3-, mGluR7a-, and mGluR8-LI were reduced markedly in the target
regions on the side ipsilateral to the transection; no significant changes were
detected in mGluR1- or mGIuR5-LI. Double labeling experiments indicated light
and electron microscopically colocalization of mGluR7a- and mGluRS-LI in axon
terminals on dendritic shafts of presumed interneurons in the superficial layer
of the olfactory tubercle and layer Ia of the piriform cortex. Electron microscopically
mGluR2/3-LI was seen in preterminal and terminal portions of axons, whereas mGluR7a-
and mGluRS-LI were associated with presynaptic membrane specialization. Immunolabeled
axon terminals were filled with round synaptic vesicles and constituted asymmetric
synapses with dendritic profiles. The results suggest that glutamate release from
axon terminals of projection fibers from MOB and AOB is regulated presynaptically
and differentially through mGluR2/3, mGluR7a, and/or mGluRS.
acknowledgement: The authors are grateful for photographic help of Mr.Akira
Uesugi. The authors also express their gratitudesfor the support of Dr. Satoru Fukuchi,
Dr. Ritsu Hayashi,Dr. Sohzaburo Hayashi, Dr. Mizuho Katsurada, Dr. Hitoshi Kawai,
Dr. Yutaka Kitani, Dr. Toshihiko Kuroda, Dr.Keiko Kumagai, Dr. Hiroshi Matsubara,
Dr. Hiroshi Matsushima, Dr. Chisato Minakuchi, Dr. Gonpei Niwa, Dr.Hajime Oda,
Dr. Mashiko Ohbayashi, Dr. Seiichi Ohbayashi, Dr. Hiroyasu Ohtsuka, Dr. Shigeo Tamaki,
Dr. EizoWatanabe, Dr. Kazuo Yoshino, and Dr. Toshiaki Yoshino.
article_processing_charge: No
article_type: original
author:
- first_name: Eiki
full_name: Wada, Eiki
last_name: Wada
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Ayae
full_name: Kinoshita, Ayae
last_name: Kinoshita
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Wada E, Shigemoto R, Kinoshita A, Ohishi H, Mizuno N. Metabotropic glutamate
receptor subtypes in axon terminals of projection fibers from the main and accessory
olfactory bulbs: A light and electron microscopic immunohistochemical study in
the rat. Journal of Comparative Neurology. 1998;393(4):493-504. doi:10.1002/(SICI)1096-9861(19980420)393:4<493::AID-CNE8>3.0.CO;2-W'
apa: 'Wada, E., Shigemoto, R., Kinoshita, A., Ohishi, H., & Mizuno, N. (1998).
Metabotropic glutamate receptor subtypes in axon terminals of projection fibers
from the main and accessory olfactory bulbs: A light and electron microscopic
immunohistochemical study in the rat. Journal of Comparative Neurology.
Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19980420)393:4<493::AID-CNE8>3.0.CO;2-W'
chicago: 'Wada, Eiki, Ryuichi Shigemoto, Ayae Kinoshita, Hitoshi Ohishi, and Noboru
Mizuno. “Metabotropic Glutamate Receptor Subtypes in Axon Terminals of Projection
Fibers from the Main and Accessory Olfactory Bulbs: A Light and Electron Microscopic
Immunohistochemical Study in the Rat.” Journal of Comparative Neurology.
Wiley-Blackwell, 1998. https://doi.org/10.1002/(SICI)1096-9861(19980420)393:4<493::AID-CNE8>3.0.CO;2-W.'
ieee: 'E. Wada, R. Shigemoto, A. Kinoshita, H. Ohishi, and N. Mizuno, “Metabotropic
glutamate receptor subtypes in axon terminals of projection fibers from the main
and accessory olfactory bulbs: A light and electron microscopic immunohistochemical
study in the rat,” Journal of Comparative Neurology, vol. 393, no. 4. Wiley-Blackwell,
pp. 493–504, 1998.'
ista: 'Wada E, Shigemoto R, Kinoshita A, Ohishi H, Mizuno N. 1998. Metabotropic
glutamate receptor subtypes in axon terminals of projection fibers from the main
and accessory olfactory bulbs: A light and electron microscopic immunohistochemical
study in the rat. Journal of Comparative Neurology. 393(4), 493–504.'
mla: 'Wada, Eiki, et al. “Metabotropic Glutamate Receptor Subtypes in Axon Terminals
of Projection Fibers from the Main and Accessory Olfactory Bulbs: A Light and
Electron Microscopic Immunohistochemical Study in the Rat.” Journal of Comparative
Neurology, vol. 393, no. 4, Wiley-Blackwell, 1998, pp. 493–504, doi:10.1002/(SICI)1096-9861(19980420)393:4<493::AID-CNE8>3.0.CO;2-W.'
short: E. Wada, R. Shigemoto, A. Kinoshita, H. Ohishi, N. Mizuno, Journal of Comparative
Neurology 393 (1998) 493–504.
date_created: 2018-12-11T11:58:31Z
date_published: 1998-04-20T00:00:00Z
date_updated: 2022-08-31T14:53:58Z
day: '20'
doi: 10.1002/(SICI)1096-9861(19980420)393:4<493::AID-CNE8>3.0.CO;2-W
extern: '1'
external_id:
pmid:
- '9550154'
intvolume: ' 393'
issue: '4'
language:
- iso: eng
month: '04'
oa_version: None
page: 493 - 504
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4313'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Metabotropic glutamate receptor subtypes in axon terminals of projection fibers
from the main and accessory olfactory bulbs: A light and electron microscopic immunohistochemical
study in the rat'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 393
year: '1998'
...
---
_id: '2589'
abstract:
- lang: eng
text: Immunoreactivity for the substance P receptor (NK1 receptor) has been investigated
by light and electron microscopy in the dorsal vagal complexes of adult rats and
cats. The general pattern of NK1 immunoreactivity was similar for both rat and
cat. Numerous NK1-immunoreactive neurons were present in the area postrema, the
nucleus of the solitary tract, and the dorsal motor nucleus of the vagus nerve.
The density of labelled neurons differed between the subnuclei of the nucleus
of the solitary tract. Overall, the efferent neurons of the dorsal motor nucleus
of the vagus nerve highly expressed NK1 when compared to neurons in the nucleus
of the solitary tract. The results are discussed with reference to the viscerotopic
organisation of the dorsal vagal complex. Ultrastructural analysis demonstrated
that NK1 immunoreactivity was present only at the membrane surface of somatic
and dendritic profiles of neurons. No labelling was found in axon terminals, axons,
or glial processes. NK1 immunoreactivity, as revealed by a preembedding immunogold
technique in serial ultrathin sections; was preferentially located at nonsynaptic
sites. A semiquantitative study suggested that the density of NK1 receptors is
statistically higher at membrane sites free of any contact (synaptic or not) with
axon terminals. The subcellular localisation of NK1 immunoreactivity was similar
for neurons of both rat and cat. These results suggest that in the dorsal vagal
complex, substance P might act on NK1 receptors through a process of volume transmission.
acknowledgement: The authors thank Dr. Wolfgang A.A. Kunze for his helpin the English
reviewing of the manuscript. The authorsthank Drs. Nadine Clerc, Jean-Pierre Kessler,
WolfgangA.A. Kunze, Jean-Jacques Puizillout, and Fabien Tell fortheir constructive
discussions and critiques of the manu-script. This study was supported by CNRS (FR45/UPR9024).
article_processing_charge: No
article_type: original
author:
- first_name: Agnès
full_name: Baude, Agnès
last_name: Baude
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: 'Baude A, Shigemoto R. Cellular and subcellular distribution of substance P
receptor immunoreactivity in the dorsal vagal complex of the rat and cat: A light
and electron microscope study. Journal of Comparative Neurology. 1998;402(2):181-196.
doi:10.1002/(SICI)1096-9861(19981214)402:2<181::AID-CNE4>3.0.CO;2-B'
apa: 'Baude, A., & Shigemoto, R. (1998). Cellular and subcellular distribution
of substance P receptor immunoreactivity in the dorsal vagal complex of the rat
and cat: A light and electron microscope study. Journal of Comparative Neurology.
Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19981214)402:2<181::AID-CNE4>3.0.CO;2-B'
chicago: 'Baude, Agnès, and Ryuichi Shigemoto. “Cellular and Subcellular Distribution
of Substance P Receptor Immunoreactivity in the Dorsal Vagal Complex of the Rat
and Cat: A Light and Electron Microscope Study.” Journal of Comparative Neurology.
Wiley-Blackwell, 1998. https://doi.org/10.1002/(SICI)1096-9861(19981214)402:2<181::AID-CNE4>3.0.CO;2-B.'
ieee: 'A. Baude and R. Shigemoto, “Cellular and subcellular distribution of substance
P receptor immunoreactivity in the dorsal vagal complex of the rat and cat: A
light and electron microscope study,” Journal of Comparative Neurology,
vol. 402, no. 2. Wiley-Blackwell, pp. 181–196, 1998.'
ista: 'Baude A, Shigemoto R. 1998. Cellular and subcellular distribution of substance
P receptor immunoreactivity in the dorsal vagal complex of the rat and cat: A
light and electron microscope study. Journal of Comparative Neurology. 402(2),
181–196.'
mla: 'Baude, Agnès, and Ryuichi Shigemoto. “Cellular and Subcellular Distribution
of Substance P Receptor Immunoreactivity in the Dorsal Vagal Complex of the Rat
and Cat: A Light and Electron Microscope Study.” Journal of Comparative Neurology,
vol. 402, no. 2, Wiley-Blackwell, 1998, pp. 181–96, doi:10.1002/(SICI)1096-9861(19981214)402:2<181::AID-CNE4>3.0.CO;2-B.'
short: A. Baude, R. Shigemoto, Journal of Comparative Neurology 402 (1998) 181–196.
date_created: 2018-12-11T11:58:32Z
date_published: 1998-12-14T00:00:00Z
date_updated: 2022-08-31T12:57:30Z
day: '14'
doi: 10.1002/(SICI)1096-9861(19981214)402:2<181::AID-CNE4>3.0.CO;2-B
extern: '1'
external_id:
pmid:
- '9845242 '
intvolume: ' 402'
issue: '2'
language:
- iso: eng
month: '12'
oa_version: None
page: 181 - 196
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4310'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Cellular and subcellular distribution of substance P receptor immunoreactivity
in the dorsal vagal complex of the rat and cat: A light and electron microscope
study'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 402
year: '1998'
...
---
_id: '2493'
abstract:
- lang: eng
text: 'A specific antiserum against substance P receptor (SPR) labels nonprincipal
neurons in the cerebral cortex of the rat (T. Kaneko et al. [1994], Neuroscience
60:199-211; Y. Nakaya et al. [1994], J. Comp. Neurol. 347:249-274). In the present
study, we aimed to identify the types of SPR- immunoreactive neurons in the hippocampus
according to their content of neurochemical markers, which label interneuron populations
with distinct termination patterns. Markers for perisomatic inhibitory cells,
parvalbumin and cholecystokinin (CCK), colocalized with SPR in pyramidallike basket
cells in the dentate gyrus and in large multipolar or bitufted cells within all
hippocampal subfields respectively. A dense meshwork of SPR-immunoreactive spiny
dendrites in the hilus and stratum lucidum of the CA3 region belonged largely
to inhibitory cells terminating in the distal dendritic region of granule cells,
as indicated by the somatostatin and neuropeptide Y (NPY) content. In addition,
SPR and NPY were colocalized in numerous multipolar interneurons with dendrites
branching close to the soma. Twenty-five percent of the SPR-immunoreactive cells
overlapped with calretinin-positive neurons in all hippocampal subfields, showing
that interneurons specialized to contact other gamma-aminobutyric acid-ergic cells
may also contain SPR. On the basis of the known termination pattern of the colocalized
markers, we conclude that SPR-positive interneurons are functionally heterogeneous
and participate in different inhibitory processes: (1) perisomatic inhibition
of principal cells (CCK-containing cells, and parvalbumin-positive cells in the
dentate gyrus), (2) feedback dendritic inhibition in the entorhinal termination
zone (somatostatin and NPY-containing cells), and (3) innervation of other interneurons
(calretinin-containing cells).'
acknowledgement: This sudy was supported by grants from the Human Frontier Science
Program Organisation, the Howard Hughes Medical Institute, and OTKA (T 16942) Hungary.We
are grateful to Dr. K.G. Baimbridge and to Dr. M.R.Celio (calbindin and parvalbumin),
Dr. T. Go ̈rcs (CCK, VIP,NPY, and somatostatin), Dr. J.H. Rogers (calretinin), andDr.
C.G. Beaulieau (GABA) for kind gifts of antisera. The excellent technical assistance
of Mrs. E. Borok, Mrs. A.Z.Szabo, and Mr. G. Terstyanszky is also acknowledged
article_processing_charge: No
article_type: original
author:
- first_name: László
full_name: Acsády, László
last_name: Acsády
- first_name: István
full_name: Katona, István
last_name: Katona
- first_name: Attila
full_name: Gulyás, Attila
last_name: Gulyás
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Tamás
full_name: Freund, Tamás
last_name: Freund
citation:
ama: Acsády L, Katona I, Gulyás A, Shigemoto R, Freund T. Immunostaining for substance
P receptor labels GABAergic cells with distinct termination patterns in the hippocampus.
Journal of Comparative Neurology. 1997;378(3):320-336. doi:10.1002/(SICI)1096-9861(19970217)378:3<320::AID-CNE2>3.0.CO;2-5
apa: Acsády, L., Katona, I., Gulyás, A., Shigemoto, R., & Freund, T. (1997).
Immunostaining for substance P receptor labels GABAergic cells with distinct termination
patterns in the hippocampus. Journal of Comparative Neurology. Wiley-Blackwell.
https://doi.org/10.1002/(SICI)1096-9861(19970217)378:3<320::AID-CNE2>3.0.CO;2-5
chicago: Acsády, László, István Katona, Attila Gulyás, Ryuichi Shigemoto, and Tamás
Freund. “Immunostaining for Substance P Receptor Labels GABAergic Cells with Distinct
Termination Patterns in the Hippocampus.” Journal of Comparative Neurology.
Wiley-Blackwell, 1997. https://doi.org/10.1002/(SICI)1096-9861(19970217)378:3<320::AID-CNE2>3.0.CO;2-5.
ieee: L. Acsády, I. Katona, A. Gulyás, R. Shigemoto, and T. Freund, “Immunostaining
for substance P receptor labels GABAergic cells with distinct termination patterns
in the hippocampus,” Journal of Comparative Neurology, vol. 378, no. 3.
Wiley-Blackwell, pp. 320–336, 1997.
ista: Acsády L, Katona I, Gulyás A, Shigemoto R, Freund T. 1997. Immunostaining
for substance P receptor labels GABAergic cells with distinct termination patterns
in the hippocampus. Journal of Comparative Neurology. 378(3), 320–336.
mla: Acsády, László, et al. “Immunostaining for Substance P Receptor Labels GABAergic
Cells with Distinct Termination Patterns in the Hippocampus.” Journal of Comparative
Neurology, vol. 378, no. 3, Wiley-Blackwell, 1997, pp. 320–36, doi:10.1002/(SICI)1096-9861(19970217)378:3<320::AID-CNE2>3.0.CO;2-5.
short: L. Acsády, I. Katona, A. Gulyás, R. Shigemoto, T. Freund, Journal of Comparative
Neurology 378 (1997) 320–336.
date_created: 2018-12-11T11:57:59Z
date_published: 1997-02-17T00:00:00Z
date_updated: 2022-08-22T13:43:18Z
day: '17'
doi: 10.1002/(SICI)1096-9861(19970217)378:3<320::AID-CNE2>3.0.CO;2-5
extern: '1'
external_id:
pmid:
- '9034894'
intvolume: ' 378'
issue: '3'
language:
- iso: eng
month: '02'
oa_version: None
page: 320 - 336
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4408'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Immunostaining for substance P receptor labels GABAergic cells with distinct
termination patterns in the hippocampus
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 378
year: '1997'
...
---
_id: '2576'
abstract:
- lang: eng
text: Primary afferent neurons containing substance P (SP) are apparently implicated
in the transmission of noxious information from the periphery to the central nervous
system, and SP released from primary afferent neurons acts on second-order neurons
with the SP receptor (SPR). In the rat, nociceptive information reached the hypothalamus
not only through indirect pathways but also directly through trigeminohypothalamic
and spinohypothalamic pathways. Thus, in the present study, the distribution pattern
of trigeminohypothalamic and spinohypothalamic tract neurons showing SPR-like
immunoreactivity (SPR-LI) was examined in the rat by a retrograde tract-tracing
method combined with immunofluorescence histochemistry for SPR. A substantial
number of trigeminal and spinal neurons with SPR-LI were retrogradely labeled
with Fluore-Gold (FG) injected into the hypothalamic regions. These neurons were
distributed mainly in lamina I of the medullary and spinal dorsal horns, lateral
spinal nucleus, regions around the central canal of the spinal cord, and the lateral
aspect of the deep part of the spinal dorsal horn. A number of SPR-LI neurons
in the spinal parasympathetic nucleus were labeled with FG injected into the area
around the paraventricular hypothalamic nucleus. Some SPR-LI neurons in the lateral
spinal nucleus and the lateral aspect of the deep part of the spinal dorsal horn
were also labeled with FG injected into the septal region. On the basis of the
distribution areas of SPR-LI trigeminal and spinal neurons projecting to the hypothalamic
and septal regions, it is likely that these neurons are involved in the transmission
of somatic and/or visceral noxious information.
acknowledgement: This study was supported by grants 08279106 and 08458245 from the
Ministry of Education, Science, Sportsand Culture of Japan. We are grateful for
the photographic help of Mr. Akira Uesugi and the support of Dr. Kajitaro Morita
in Morita Clinic of Internal Medicine and Pediatrics, Kadoma, Osaka, Japan. We also
express our gratitude for the support of Drs. Satoru Fukuchi, Ritsu Hayashi,Sohzaburo
Hayashi, Mizuho Katsurada, Hitoski Kawai,Yutaka Kitani, Toshihiko Kuroda, Keiko
Kumagai, Hiroshi Matsubara, Hiroshi Matsushima, Chisato Minakuchi,Gonpei Niwa, Hajime
Oda, Mashiko Ohbayashi, Seiichi Ohbayashi, Hiroyasu Ohtsuka, Shigeo Tamaki, EizoWatanabe,
Kazuo Yoshino, and Toshiaki Yoshino.
article_processing_charge: No
article_type: original
author:
- first_name: Jin
full_name: Li, Jin
last_name: Li
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Li J, Kaneko T, Shigemoto R, Mizuno N. Distribution of trigeminohypothalamic
and spinohypothalamic tract neurons displaying substance P receptor-like immunoreactivity
in the rat. Journal of Comparative Neurology. 1997;378(4):508-521. doi:10.1002/(SICI)1096-9861(19970224)378:4<508::AID-CNE6>3.0.CO;2-6
apa: Li, J., Kaneko, T., Shigemoto, R., & Mizuno, N. (1997). Distribution of
trigeminohypothalamic and spinohypothalamic tract neurons displaying substance
P receptor-like immunoreactivity in the rat. Journal of Comparative Neurology.
Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19970224)378:4<508::AID-CNE6>3.0.CO;2-6
chicago: Li, Jin, Takeshi Kaneko, Ryuichi Shigemoto, and Noboru Mizuno. “Distribution
of Trigeminohypothalamic and Spinohypothalamic Tract Neurons Displaying Substance
P Receptor-like Immunoreactivity in the Rat.” Journal of Comparative Neurology.
Wiley-Blackwell, 1997. https://doi.org/10.1002/(SICI)1096-9861(19970224)378:4<508::AID-CNE6>3.0.CO;2-6.
ieee: J. Li, T. Kaneko, R. Shigemoto, and N. Mizuno, “Distribution of trigeminohypothalamic
and spinohypothalamic tract neurons displaying substance P receptor-like immunoreactivity
in the rat,” Journal of Comparative Neurology, vol. 378, no. 4. Wiley-Blackwell,
pp. 508–521, 1997.
ista: Li J, Kaneko T, Shigemoto R, Mizuno N. 1997. Distribution of trigeminohypothalamic
and spinohypothalamic tract neurons displaying substance P receptor-like immunoreactivity
in the rat. Journal of Comparative Neurology. 378(4), 508–521.
mla: Li, Jin, et al. “Distribution of Trigeminohypothalamic and Spinohypothalamic
Tract Neurons Displaying Substance P Receptor-like Immunoreactivity in the Rat.”
Journal of Comparative Neurology, vol. 378, no. 4, Wiley-Blackwell, 1997,
pp. 508–21, doi:10.1002/(SICI)1096-9861(19970224)378:4<508::AID-CNE6>3.0.CO;2-6.
short: J. Li, T. Kaneko, R. Shigemoto, N. Mizuno, Journal of Comparative Neurology
378 (1997) 508–521.
date_created: 2018-12-11T11:58:28Z
date_published: 1997-02-24T00:00:00Z
date_updated: 2022-08-22T13:34:53Z
day: '24'
doi: 10.1002/(SICI)1096-9861(19970224)378:4<508::AID-CNE6>3.0.CO;2-6
extern: '1'
external_id:
pmid:
- '9034907'
intvolume: ' 378'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 508 - 521
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4323'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distribution of trigeminohypothalamic and spinohypothalamic tract neurons displaying
substance P receptor-like immunoreactivity in the rat
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 378
year: '1997'
...
---
_id: '2578'
abstract:
- lang: eng
text: The distribution of immunoreactivity to the neurokinin3 receptor (NK3R) was
examined in segments C7, T11-12, L1-2, and L4-6 of the rat spinal cord. NK3R immunoreactivity
was visualized by using two antisera generated against sequences of amino acids
contained in the C-terminal region of the NK3R. NK3R-immunoreactive cells were
numerous in the substantia gelatinosa of all spinal segments examined as well
as the dorsal commissural nucleus of spinal segments L1-2. Isolated, immunoreactive
cells were scattered throughout other regions of the spinal cord. The relationship
of NK3R-immunoreactivity with neurons was demonstrated by colocalization with
microtubule associated protein 2-immunoreactivity in individual cells. Within
neurons, NK3R- immunoreactivity was associated predominately with the plasma membrane
of cell bodies and dendrites. Within the substantia gelatinosa, 86% of nitric
oxide synthase (NOS)-immunoreactive neurons were also NK3R-immunoreactive. Although
NOS-immunoreactive neurons were found throughout all other regions of the spinal
cord in the segments examined, these were not NK3R- immunoreactive. When preganglionic
sympathetic neurons in spinal segments T11-12 and L1-2 were visualized by intraperitoneal
injection of Fluorogold, less than 1% of the Fluorogold-labeled neurons were also
immunoreactive for NK3R. The large number of NK3R-immunoreactive neurons in the
substantia gelatinosa suggests that some effects of tachykinins an somatosensation
may be mediated by NK3R.
acknowledgement: The authors are grateful to Dr. Colin Anderson fordiscussions of
the organization of spinal autonomic nuclei.V.S.S. was a visiting research fellow
in the Department ofAnatomy and Cell Biology, University of Melbourne.
article_processing_charge: No
article_type: original
author:
- first_name: Virginia
full_name: Seybold, Virginia
last_name: Seybold
- first_name: Ivica
full_name: Grković, Ivica
last_name: Grković
- first_name: Andrea
full_name: Portbury, Andrea
last_name: Portbury
- first_name: Yu
full_name: Ding, Yu
last_name: Ding
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
- first_name: John
full_name: Furness, John
last_name: Furness
- first_name: Bridget
full_name: Southwell, Bridget
last_name: Southwell
citation:
ama: Seybold V, Grković I, Portbury A, et al. Relationship of NK3 receptor-immunoreactivity
to subpopulations of neurons in rat spinal cord. Journal of Comparative Neurology.
1997;381(4):439-448. doi:10.1002/(SICI)1096-9861(19970519)381:4<439::AID-CNE4>3.0.CO;2-3
apa: Seybold, V., Grković, I., Portbury, A., Ding, Y., Shigemoto, R., Mizuno, N.,
… Southwell, B. (1997). Relationship of NK3 receptor-immunoreactivity to subpopulations
of neurons in rat spinal cord. Journal of Comparative Neurology. Wiley-Blackwell.
https://doi.org/10.1002/(SICI)1096-9861(19970519)381:4<439::AID-CNE4>3.0.CO;2-3
chicago: Seybold, Virginia, Ivica Grković, Andrea Portbury, Yu Ding, Ryuichi Shigemoto,
Noboru Mizuno, John Furness, and Bridget Southwell. “Relationship of NK3 Receptor-Immunoreactivity
to Subpopulations of Neurons in Rat Spinal Cord.” Journal of Comparative Neurology.
Wiley-Blackwell, 1997. https://doi.org/10.1002/(SICI)1096-9861(19970519)381:4<439::AID-CNE4>3.0.CO;2-3.
ieee: V. Seybold et al., “Relationship of NK3 receptor-immunoreactivity to
subpopulations of neurons in rat spinal cord,” Journal of Comparative Neurology,
vol. 381, no. 4. Wiley-Blackwell, pp. 439–448, 1997.
ista: Seybold V, Grković I, Portbury A, Ding Y, Shigemoto R, Mizuno N, Furness J,
Southwell B. 1997. Relationship of NK3 receptor-immunoreactivity to subpopulations
of neurons in rat spinal cord. Journal of Comparative Neurology. 381(4), 439–448.
mla: Seybold, Virginia, et al. “Relationship of NK3 Receptor-Immunoreactivity to
Subpopulations of Neurons in Rat Spinal Cord.” Journal of Comparative Neurology,
vol. 381, no. 4, Wiley-Blackwell, 1997, pp. 439–48, doi:10.1002/(SICI)1096-9861(19970519)381:4<439::AID-CNE4>3.0.CO;2-3.
short: V. Seybold, I. Grković, A. Portbury, Y. Ding, R. Shigemoto, N. Mizuno, J.
Furness, B. Southwell, Journal of Comparative Neurology 381 (1997) 439–448.
date_created: 2018-12-11T11:58:29Z
date_published: 1997-05-19T00:00:00Z
date_updated: 2022-08-22T12:27:14Z
day: '19'
doi: 10.1002/(SICI)1096-9861(19970519)381:4<439::AID-CNE4>3.0.CO;2-3
extern: '1'
external_id:
pmid:
- '9136801 '
intvolume: ' 381'
issue: '4'
language:
- iso: eng
month: '05'
oa_version: None
page: 439 - 448
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4320'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Relationship of NK3 receptor-immunoreactivity to subpopulations of neurons
in rat spinal cord
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 381
year: '1997'
...
---
_id: '2581'
abstract:
- lang: eng
text: It is well known that striatonigral neurons produce substance P (SP); however,
no SP receptor (SPR) has so far been found in the substantia nigra. On the other
hand, a previous study in the rat striatum indicated that SPR was expressed only
in cholinergic or somatostatinergic intrinsic neurons (Kaneko et al. [1993] Brain
Res. 631:297-303). Thus, it was assumed that SP produced by striatenigral neurons
might be released through their intrastriatal axon collaterals to act upon intrinsic
neurons in the striatum. To confirm this assumption, the distribution of axon
collaterals of striatonigral neurons was examined in the striatum of the rat.
The experiments were performed on brain slices by combining retrograde labeling
with tetramethylrhodamine-dextran amine, electrophysiological recording, intracellular
staining with biocytin, and immunocytochemistry for SPR. The distribution of axons
of cholinergic striatal neurons (a group of SP-negative intrinsic striatal neurons)
was also examined. It was observed that 16% of varicosities of intrastriatal axon
collaterals of striatonigral neurons, as well as 6% of axonal varicosities of
cholinergic neurons, were in close apposition to dendrites and cell bodies of
SPB-immunoreactive striatal neurons. Since SPR-immunoreactive striatal neurons
constituted only 2.7% of the total population of striatal neurons (Kaneko et al.
[1993] Brain Res. 631:297-303), it appeared that axonal varicosities of striatonigral
neurons were preferentially apposed to SPR-immunoreactive striatal neurons and
that the varicosities in close apposition to SPR-immunoreactive neurons were derived
more frequently from striatonigral neurons than from cholinergic interneurons.
Confocal laser scanning microscopy indicated that axonal varicosities in close
apposition to SPR-immunoreactive cells showed synaptophysin immunoreactivity,
a marker of synaptic vesicles. In intrastriatal axons of striatonigral neurons,
it was further revealed from electron microscopy that axonal varicosities in close
apposition to SPR- immunoreactive dendrites, at least a part of them, made synapses
of the symmetric type. Striatonigral neurons might release SP preferentially around
cholinergic or somatostatinergic intrinsic neurons to regulate them through SP-SPR
interactions.
acknowledgement: The authors are grateful for the photographic help of Mr.A. Uesugi.
We also express our gratitude for the support ofDrs. Satoru Fukuchi, Ritsu Hayashi,
Sohzaburo Hayashi,Mizuho Katsurada, Hitoshi Kawai, Yutaka Kitani, Toshi-hiko Kuroda,
Keiko Kumagai, Hiroshi Matsubara, HiroshiMatsushima, Chisato Minakuchi, Gonpei Niwa, HajimeOda,
Masahiko Ohbayashi, Sei-ichi Ohbayashi, Hiroyasu Ohtsuka, Shigeo Tamaki, Eizo Watanabe,
Kazuo Yoshino,and Toshiaki Yoshino.
article_processing_charge: No
article_type: original
author:
- first_name: Teffy
full_name: Lee, Teffy
last_name: Lee
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Sakashi
full_name: Nomura, Sakashi
last_name: Nomura
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Lee T, Kaneko T, Shigemoto R, Nomura S, Mizuno N. Collateral projections from
striatonigral neurons to substance P receptor-expressing intrinsic neurons in
the striatum of the rat. Journal of Comparative Neurology. 1997;388(2):250-264.
doi:10.1002/(SICI)1096-9861(19971117)388:2<250::AID-CNE5>3.0.CO;2-0
apa: Lee, T., Kaneko, T., Shigemoto, R., Nomura, S., & Mizuno, N. (1997). Collateral
projections from striatonigral neurons to substance P receptor-expressing intrinsic
neurons in the striatum of the rat. Journal of Comparative Neurology. Wiley-Blackwell.
https://doi.org/10.1002/(SICI)1096-9861(19971117)388:2<250::AID-CNE5>3.0.CO;2-0
chicago: Lee, Teffy, Takeshi Kaneko, Ryuichi Shigemoto, Sakashi Nomura, and Noboru
Mizuno. “Collateral Projections from Striatonigral Neurons to Substance P Receptor-Expressing
Intrinsic Neurons in the Striatum of the Rat.” Journal of Comparative Neurology.
Wiley-Blackwell, 1997. https://doi.org/10.1002/(SICI)1096-9861(19971117)388:2<250::AID-CNE5>3.0.CO;2-0.
ieee: T. Lee, T. Kaneko, R. Shigemoto, S. Nomura, and N. Mizuno, “Collateral projections
from striatonigral neurons to substance P receptor-expressing intrinsic neurons
in the striatum of the rat,” Journal of Comparative Neurology, vol. 388,
no. 2. Wiley-Blackwell, pp. 250–264, 1997.
ista: Lee T, Kaneko T, Shigemoto R, Nomura S, Mizuno N. 1997. Collateral projections
from striatonigral neurons to substance P receptor-expressing intrinsic neurons
in the striatum of the rat. Journal of Comparative Neurology. 388(2), 250–264.
mla: Lee, Teffy, et al. “Collateral Projections from Striatonigral Neurons to Substance
P Receptor-Expressing Intrinsic Neurons in the Striatum of the Rat.” Journal
of Comparative Neurology, vol. 388, no. 2, Wiley-Blackwell, 1997, pp. 250–64,
doi:10.1002/(SICI)1096-9861(19971117)388:2<250::AID-CNE5>3.0.CO;2-0.
short: T. Lee, T. Kaneko, R. Shigemoto, S. Nomura, N. Mizuno, Journal of Comparative
Neurology 388 (1997) 250–264.
date_created: 2018-12-11T11:58:30Z
date_published: 1997-11-17T00:00:00Z
date_updated: 2022-08-22T10:03:38Z
day: '17'
doi: 10.1002/(SICI)1096-9861(19971117)388:2<250::AID-CNE5>3.0.CO;2-0
extern: '1'
external_id:
pmid:
- '9368840'
intvolume: ' 388'
issue: '2'
language:
- iso: eng
month: '11'
oa_version: None
page: 250 - 264
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4316'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Collateral projections from striatonigral neurons to substance P receptor-expressing
intrinsic neurons in the striatum of the rat
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 388
year: '1997'
...
---
_id: '2564'
abstract:
- lang: eng
text: The distribution of the neuromedin K receptor (NK3; NKR) in the central nervous
system was investigated in the adult rat by using in situ hybridization and immunohistochemical
techniques. The rabbit anti-NKR antibody was raised against a bacterial fusion
protein containing a C- terminal portion of NKR and affinity purified with a Sepharose
4B column conjugated to the fusion protein. Immunoblot analysis was performed
to test the reactivity and specificity of the antibody. Crude membrane was prepared
from cDNA-transfected Chinese hamster ovary (CHO) cells expressing each of the
rat NKR, substance P receptor (NK1; SPR), and substance K receptor (NK2; SKR)
and from the hypothalamus, cerebral cortex, and cerebellum. Immunoreactive bands
were observed specifically in the NKR-CHO cells, hypothalamus, and cerebral cortex
but not in the SPR- or SKR-CHO cells, nor in the cerebellum. Molecular weights
of the immunoreactive bands ranged from 73 to 89 kDa and from 59 to 83 kDa in
the NKR-CHO cells and tissues, respectively. The distribution of NKR-like immunoreactivity
coincided with that of NKR mRNA. The expression of NKR was indicated on neuronal
cell bodies and dendrites. NKR was found to be expressed intensely or moderately
in neurons in the glomerular and granule cell layers of the main olfactory bulb;
glomerular and mitral cell layers of the accessory olfactory bulb; layers IV and
V of the cerebral neocortex; medial septal nucleus; nucleus of the diagonal band;
bed nucleus of the stria terminalis; globus pallidus; ventral pallidum; paraventricular
nucleus; supraoptic nucleus; zona incerta; dorsal, lateral, and posterior hypothalamic
areas; amygdaloid nuclei; medial habenular nucleus; ventral tegmental area; midbrain
periaqueductal gray; interpeduncular nuclei; substantia nigra pars compacta; linear,
median, dorsal, and pontine raphe nuclei; posteromedial tegmental nucleus; sphenoid
nucleus; nucleus of the solitary tract; intermediate and rostroventrolateral reticular
nuclei; and lamina II of the caudal spinal trigeminal nucleus and spinal dorsal
horn. These findings are discussed in relation to the physiological functions
associated with neuromedin K.
acknowledgement: 'We are grateful for the photographic help of Mr. A. Uesugi and
the support of Drs. Satoru Fukuchi, Ritsu Hayashi, Sozaburo Hayashi, Mizuho Katsurada,
Yutaka Kitani, Keiko Kumagai, Toshihiko Kuroda, Hiroshi Matsu- bara, Hiroshi Matsushima, Chisato
Minakuchi, Gonpei Niwa, Hajime Oda, Masahiko Ohbayashi, Sei-ichi Ohbayashi,
Hiroyasu Ohtsuka, Shigeo Tamaki, Eizo Watanabe, Kazuo Yoshino, and Toshiaki Yoshino.
This work was supported in part by Grants-in-Aid for Special Research on Priority
areas 05267104, Scientific Research (B) 05454658, and Scientific Research (C) 06680735
from the Ministry of Education, Science and Culture of Japan. '
article_processing_charge: No
article_type: original
author:
- first_name: Yu
full_name: Ding, Yu
last_name: Ding
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Masahiko
full_name: Takada, Masahiko
last_name: Takada
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Ding Y, Shigemoto R, Takada M, Ohishi H, Nakanishi S, Mizuno N. Localization
of the neuromedin K receptor (NK3) in the central nervous system of the rat. Journal
of Comparative Neurology. 1996;364(2):290-310. doi:10.1002/(SICI)1096-9861(19960108)364:2<290::AID-CNE8>3.0.CO;2-0
apa: Ding, Y., Shigemoto, R., Takada, M., Ohishi, H., Nakanishi, S., & Mizuno,
N. (1996). Localization of the neuromedin K receptor (NK3) in the central nervous
system of the rat. Journal of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19960108)364:2<290::AID-CNE8>3.0.CO;2-0
chicago: Ding, Yu, Ryuichi Shigemoto, Masahiko Takada, Hitoshi Ohishi, Shigetada
Nakanishi, and Noboru Mizuno. “Localization of the Neuromedin K Receptor (NK3)
in the Central Nervous System of the Rat.” Journal of Comparative Neurology.
Wiley-Blackwell, 1996. https://doi.org/10.1002/(SICI)1096-9861(19960108)364:2<290::AID-CNE8>3.0.CO;2-0.
ieee: Y. Ding, R. Shigemoto, M. Takada, H. Ohishi, S. Nakanishi, and N. Mizuno,
“Localization of the neuromedin K receptor (NK3) in the central nervous system
of the rat,” Journal of Comparative Neurology, vol. 364, no. 2. Wiley-Blackwell,
pp. 290–310, 1996.
ista: Ding Y, Shigemoto R, Takada M, Ohishi H, Nakanishi S, Mizuno N. 1996. Localization
of the neuromedin K receptor (NK3) in the central nervous system of the rat. Journal
of Comparative Neurology. 364(2), 290–310.
mla: Ding, Yu, et al. “Localization of the Neuromedin K Receptor (NK3) in the Central
Nervous System of the Rat.” Journal of Comparative Neurology, vol. 364,
no. 2, Wiley-Blackwell, 1996, pp. 290–310, doi:10.1002/(SICI)1096-9861(19960108)364:2<290::AID-CNE8>3.0.CO;2-0.
short: Y. Ding, R. Shigemoto, M. Takada, H. Ohishi, S. Nakanishi, N. Mizuno, Journal
of Comparative Neurology 364 (1996) 290–310.
date_created: 2018-12-11T11:58:25Z
date_published: 1996-01-08T00:00:00Z
date_updated: 2022-08-12T09:48:24Z
day: '08'
doi: 10.1002/(SICI)1096-9861(19960108)364:2<290::AID-CNE8>3.0.CO;2-0
extern: '1'
external_id:
pmid:
- '8788251 '
intvolume: ' 364'
issue: '2'
language:
- iso: eng
month: '01'
oa_version: None
page: 290 - 310
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4334'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Localization of the neuromedin K receptor (NK3) in the central nervous system
of the rat
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 364
year: '1996'
...
---
_id: '2572'
abstract:
- lang: eng
text: The distribution of the mRNA for a pituitary adenylate cyclase- activating
polypeptide (PACAP) receptor (PACAP-R) was examined in the rat brain, and also
in the hypophysis and pineal gland, by in situ hybridization with a specific 35S-labeled
riboprobe which was generated from a rat PACAP-R cDNA clone. In the brain, expression
of PACAP-R mRNA was most prominent in the periglomerular and granule cells of
the olfactory bulb, granule cells of the dentate gyrus, supraoptic nucleus, and
area postrema. The expression was also intense in the piriform, cingulate, and
retrosplenial cortices, pyramidal cells in CA2, non-pyramidal cells in CA1- CA3,
neuronal cells in the hilus of the dentate gyrus, lateral septal nucleus, intercalated
amygdaloid nucleus, anterodorsal thalamic nucleus, most of the midline and intralaminar
thalamic nuclei, many regions of the hypothalamus, dorsal motor nucleus of the
vagus nerve, hypoglossal nucleus, and lateral reticular nucleus. No significant
expression was detected in the mitral and tufted cells in the olfactory bulb,
pyramidal cells in CA1 and CA3, posterior nuclear group of the thalamus, dorsal
lateral geniculate nucleus, and Purkinje, Golgi, and granule cells in the cerebellar
cortex. Moderate-to-weak expression was further observed in many other regions
of the brain. In the cerebellar cortex, presumed Bergmann gila cells showed moderate
expression. In the hypophysis, the expression was moderate in the anterior lobe,
and weak to moderate in the posterior lobe; no significant expression was observed
in the intermediate lobe. In the pineal gland, the expression was very weak, if
any. Thus, the expression of PACAP-R was detected not only on neuronal cells but
also on some particular glial cells. The present study has shown, for the first
time, the exact site of PACAP-R expression in the brain and hypophysis. Although
the functional significance of PACAP and PACAP-R in the brain still remains to
be clarified, the present results are considered to provide some direction for
future functional studies.
acknowledgement: We are grateful for the photographic help of Mr. Akira Uesugi and
helpful discussions and support of Drs. Shige- tada Nakanishi, Yukihiko Sugimoto,
Atsushi Ichikawa, Masabumi Minami, Takeshi Ishihara, Jun Ogasawara, Daisuke Watanabe,
Akiko Tani, Yoshihiro Yoshihara, Miwa Kawasaki, Hiroshi Aino, Nobuya Ogawa, Akiko
Nishino, and Rie Hosoi. We also thank Ms. Yukiko Sakagami for secretarial assistance.
This work was supported in part by a Grant-in-Aid for Scientific Research from the
Ministry of We are grateful for the photographic help of Mr. Akira Uesugi and helpful discussions
and support of Drs. Shige- tada Nakanishi, Yukihiko Sugimoto, Atsushi Ichikawa,
Masabumi Minami, Takeshi Ishihara, Jun Ogasawara, Daisuke Watanabe, Akiko Tani,
Yoshihiro Yoshihara, Miwa Kawasaki, Hiroshi Aino, Nobuya Ogawa, Akiko Nishino,
and Rie Hosoi. We also thank Ms. Yukiko Sakagami for secretarial assistance. This
work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry
of We are grateful for the photographic help of Mr. Akira Uesugi and helpful discussions
and support of Drs. Shige- tada Nakanishi, Yukihiko Sugimoto, Atsushi Ichikawa,
Masabumi Minami, Takeshi Ishihara, Jun Ogasawara, Daisuke Watanabe, Akiko Tani,
Yoshihiro Yoshihara, Miwa Kawasaki, Hiroshi Aino, Nobuya Ogawa, Akiko Nishino,
and Rie Hosoi. We also thank Ms. Yukiko Sakagami for secretarial assistance. This
work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry
of Education, Science and Culture of Japan and by grants from Uehara Memorial Foundation
and Ono Pharmaceutical Co., Ltd
article_processing_charge: No
article_type: original
author:
- first_name: Hitoshi
full_name: Hashimoto, Hitoshi
last_name: Hashimoto
- first_name: Hiroyuki
full_name: Nogi, Hiroyuki
last_name: Nogi
- first_name: Kensaku
full_name: Mori, Kensaku
last_name: Mori
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Kyohei
full_name: Yamamoto, Kyohei
last_name: Yamamoto
- first_name: Toshio
full_name: Matsuda, Toshio
last_name: Matsuda
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
- first_name: Shigekazu
full_name: Nagata, Shigekazu
last_name: Nagata
- first_name: Akemichi
full_name: Baba, Akemichi
last_name: Baba
citation:
ama: 'Hashimoto H, Nogi H, Mori K, et al. Distribution of the mRNA for a pituitary
adenylate cyclase-activating polypeptide receptor in the rat brain: An in situ
hybridization study. Journal of Comparative Neurology. 1996;371(4):567-577.
doi:10.1002/(SICI)1096-9861(19960805)371:4<567::AID-CNE6>3.3.CO;2-M'
apa: 'Hashimoto, H., Nogi, H., Mori, K., Ohishi, H., Shigemoto, R., Yamamoto, K.,
… Baba, A. (1996). Distribution of the mRNA for a pituitary adenylate cyclase-activating
polypeptide receptor in the rat brain: An in situ hybridization study. Journal
of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19960805)371:4<567::AID-CNE6>3.3.CO;2-M'
chicago: 'Hashimoto, Hitoshi, Hiroyuki Nogi, Kensaku Mori, Hitoshi Ohishi, Ryuichi
Shigemoto, Kyohei Yamamoto, Toshio Matsuda, Noboru Mizuno, Shigekazu Nagata, and
Akemichi Baba. “Distribution of the MRNA for a Pituitary Adenylate Cyclase-Activating
Polypeptide Receptor in the Rat Brain: An in Situ Hybridization Study.” Journal
of Comparative Neurology. Wiley-Blackwell, 1996. https://doi.org/10.1002/(SICI)1096-9861(19960805)371:4<567::AID-CNE6>3.3.CO;2-M.'
ieee: 'H. Hashimoto et al., “Distribution of the mRNA for a pituitary adenylate
cyclase-activating polypeptide receptor in the rat brain: An in situ hybridization
study,” Journal of Comparative Neurology, vol. 371, no. 4. Wiley-Blackwell,
pp. 567–577, 1996.'
ista: 'Hashimoto H, Nogi H, Mori K, Ohishi H, Shigemoto R, Yamamoto K, Matsuda T,
Mizuno N, Nagata S, Baba A. 1996. Distribution of the mRNA for a pituitary adenylate
cyclase-activating polypeptide receptor in the rat brain: An in situ hybridization
study. Journal of Comparative Neurology. 371(4), 567–577.'
mla: 'Hashimoto, Hitoshi, et al. “Distribution of the MRNA for a Pituitary Adenylate
Cyclase-Activating Polypeptide Receptor in the Rat Brain: An in Situ Hybridization
Study.” Journal of Comparative Neurology, vol. 371, no. 4, Wiley-Blackwell,
1996, pp. 567–77, doi:10.1002/(SICI)1096-9861(19960805)371:4<567::AID-CNE6>3.3.CO;2-M.'
short: H. Hashimoto, H. Nogi, K. Mori, H. Ohishi, R. Shigemoto, K. Yamamoto, T.
Matsuda, N. Mizuno, S. Nagata, A. Baba, Journal of Comparative Neurology 371 (1996)
567–577.
date_created: 2018-12-11T11:58:27Z
date_published: 1996-08-05T00:00:00Z
date_updated: 2022-08-11T13:20:31Z
day: '05'
doi: 10.1002/(SICI)1096-9861(19960805)371:4<567::AID-CNE6>3.3.CO;2-M
extern: '1'
external_id:
pmid:
- '8841910'
intvolume: ' 371'
issue: '4'
language:
- iso: eng
month: '08'
oa_version: None
page: 567 - 577
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4326'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Distribution of the mRNA for a pituitary adenylate cyclase-activating polypeptide
receptor in the rat brain: An in situ hybridization study'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 371
year: '1996'
...
---
_id: '2491'
abstract:
- lang: eng
text: The distribution of mRNAs for metabotropic glutamate receptors, mGluR4 and
mGluR7, which are highly sensitive for L-2-amino-4-phosphonobutyrate (L- AP4),
was examined in the central nervous system of the rat by in situ hybridization.
In general, the hybridization signals of mGluR7 mRNA were more widely distributed
than those of mGluR4 mRNA, and differential expression of mGluR4 mRNA and mGluR7
mRNA was clearly indicated in some brain regions. Intense or moderate expression
of mGluR4 mRNA was detected in the granule cells of the olfactory bulb and cerebellum,
whereas no significant expression of mGluR7 mRNA was found in these cells. In
other neurons or regions where mGluR7 mRNA was intensely or moderately expressed,
no significant expression of mGluR4 mRNA was observed. Such were the mitral and
tufted cells of the olfactory bulb; anterior olfactory nucleus; neocortical regions;
cingulate cortex; retrosplenial cortex; piriform cortex; perirhinal cortex; CA1;
CA3; granule cells of the dentate gyrus; superficial layers of the subicular cortex;
deep layers of the entorhinal, parasubicular, and presubicular cortices; ventral
part of the lateral septal nucleus; septohippocampal nucleus; triangular septal
nucleus; nuclei of the diagonal band; bed nucleus of the stria terminalis; ventral
pallidum; claustrum; amygdaloid nuclei other than the intercalated nuclei; preoptic
region; hypothalamic nuclei other than the medial mammillary nucleus; ventral
lateral geniculate nucleus; locus coeruleus; Purkinje cells; many nuclei of the
lower brainstem other than the superior colliculus, periaqueductal gray, interpeduncular
nucleus, pontine nuclei, and dorsal cochlear nucleus; and dorsal horn of the spinal
cord. Both mGluR4 mRNA and mGluR7 mRNA were moderately or intensely expressed
in the olfactory tubercle, superficial layers of the entorhinal cortex, CA4, septofimbrial
nucleus, intercalated nuclei of the amygdala, medial mammillary nucleus, many
thalamic nuclei, and pontine nuclei. Intense expression of both mGluR4 mRNA and
mGluR7 mRNA was further detected in the trigeminal ganglion and dorsal root ganglia,
whereas no significant expression of them was found in the pterygopalatine ganglion
and superior cervical ganglion. The results indicate differential roles of the
L-AP4-sensitive metabotropic glutamate receptors in the glutamatergic nervous
system.
acknowledgement: We are grateful to Akira Uesugi for photographic help. This work
has been supported in part by research grants from the Ministry of Education, Science
and Culture of Japan.
article_processing_charge: No
article_type: original
author:
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Chihiro
full_name: Akazawa, Chihiro
last_name: Akazawa
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Ohishi H, Akazawa C, Shigemoto R, Nakanishi S, Mizuno N. Distributions of the
mRNAs for L-2-amino-4-phosphonobutyrate-sensitive metabotropic glutamate receptors,
mGluR4 and mGluR7, in the rat brain. Journal of Comparative Neurology.
1995;360(4):555-570. doi:10.1002/cne.903600402
apa: Ohishi, H., Akazawa, C., Shigemoto, R., Nakanishi, S., & Mizuno, N. (1995).
Distributions of the mRNAs for L-2-amino-4-phosphonobutyrate-sensitive metabotropic
glutamate receptors, mGluR4 and mGluR7, in the rat brain. Journal of Comparative
Neurology. Wiley-Blackwell. https://doi.org/10.1002/cne.903600402
chicago: Ohishi, Hitoshi, Chihiro Akazawa, Ryuichi Shigemoto, Shigetada Nakanishi,
and Noboru Mizuno. “Distributions of the MRNAs for L-2-Amino-4-Phosphonobutyrate-Sensitive
Metabotropic Glutamate Receptors, MGluR4 and MGluR7, in the Rat Brain.” Journal
of Comparative Neurology. Wiley-Blackwell, 1995. https://doi.org/10.1002/cne.903600402.
ieee: H. Ohishi, C. Akazawa, R. Shigemoto, S. Nakanishi, and N. Mizuno, “Distributions
of the mRNAs for L-2-amino-4-phosphonobutyrate-sensitive metabotropic glutamate
receptors, mGluR4 and mGluR7, in the rat brain,” Journal of Comparative Neurology,
vol. 360, no. 4. Wiley-Blackwell, pp. 555–570, 1995.
ista: Ohishi H, Akazawa C, Shigemoto R, Nakanishi S, Mizuno N. 1995. Distributions
of the mRNAs for L-2-amino-4-phosphonobutyrate-sensitive metabotropic glutamate
receptors, mGluR4 and mGluR7, in the rat brain. Journal of Comparative Neurology.
360(4), 555–570.
mla: Ohishi, Hitoshi, et al. “Distributions of the MRNAs for L-2-Amino-4-Phosphonobutyrate-Sensitive
Metabotropic Glutamate Receptors, MGluR4 and MGluR7, in the Rat Brain.” Journal
of Comparative Neurology, vol. 360, no. 4, Wiley-Blackwell, 1995, pp. 555–70,
doi:10.1002/cne.903600402.
short: H. Ohishi, C. Akazawa, R. Shigemoto, S. Nakanishi, N. Mizuno, Journal of
Comparative Neurology 360 (1995) 555–570.
date_created: 2018-12-11T11:57:59Z
date_published: 1995-10-02T00:00:00Z
date_updated: 2022-06-29T13:08:19Z
day: '02'
doi: 10.1002/cne.903600402
extern: '1'
external_id:
pmid:
- '8801249'
intvolume: ' 360'
issue: '4'
language:
- iso: eng
main_file_link:
- url: https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.903600402
month: '10'
oa_version: None
page: 555 - 570
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4410'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distributions of the mRNAs for L-2-amino-4-phosphonobutyrate-sensitive metabotropic
glutamate receptors, mGluR4 and mGluR7, in the rat brain
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 360
year: '1995'
...
---
_id: '2489'
abstract:
- lang: eng
text: 'Five N-methyl-D-aspartate (NMDA) receptor subunits have been identified thus
far: NR1, NR2A, NR2B, NR2C, and NR2D. Here, we have analyzed the expression patterns
of mRNAs for the NMDA receptor subunits in the developing and adult rats by in
situ hybridization. The developmental changes of the expression patterns were
most salient in the cerebellum. In the external granular layer, hybridization
signals of mRNAs for NR1, NR2A, NR2B, and NR2C appeared by postnatal day 3, but
no NR2D mRNA was expressed at any developmental stage examined. The NR1 mRNA was
expressed in all cerebellar neurons at all developmental stages examined. The
signals for the NR2A mRNA appeared in Purkinje cells and granule cells during
the second postnatal week. The signals for the NR2B mRNA in granule cells were
seen transiently during the first 2 weeks after birth. The signals for NR2C mRNA
appeared in granule cells and glial cells during the second postnatal week. The
signals for NR2D mRNA appeared transiently in Purkinje cells during the first
8 postnatal days; in adult rats, these were seen in stellate and Golgi cells.
In the cerebellar nuclei, mRNAs for NR1, NR2A, NR2B, and NR2D were more or less
expressed on postnatal day 0, while expression signals for the NR2C mRNA were
first detected in postnatal day 14. Thus, the most conspicuous changes of expression
patterns were observed in the cerebellar cortex during the first 2 weeks after
birth, when development and maturation of the cerebellum proceed most rapidly.'
acknowledgement: We are grateful to Mr. Akira Uesugi for photographic help and
for the support of Drs. Satoru Fukuchi, Toshio Fukuda, Ritsu Hayashi, Mizuho Katsurada,
Yutaka Kitani, Keiko Kumagai, Toshihiko Kuroda, Hiroshi Matsubara, Hiroshi Matsushima,
Chisato Minakuchi, Masatoshi Nishio, Gonpei Niwa Hajime Oda, Masahiko Ohbayashi,
Sei-ichi Ohbayashi, Hiroyasu Ohtsuka, Shigeo Tamaki, Eizo Watan- abe, Kazuo Yoshino,
and Toshiaki Yoshino. This work was supported in part by research grants from the
Ministry of Education, Science and Culture of Japan.
article_processing_charge: No
article_type: original
author:
- first_name: Chihiro
full_name: Akazawa, Chihiro
last_name: Akazawa
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Yasumasa
full_name: Bessho, Yasumasa
last_name: Bessho
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Akazawa C, Shigemoto R, Bessho Y, Nakanishi S, Mizuno N. Differential expression
of five N-methyl-D-aspartate receptor subunit mRNAs in the cerebellum of developing
and adult rats. Journal of Comparative Neurology. 1994;347(1):150-160.
doi:10.1002/cne.903470112
apa: Akazawa, C., Shigemoto, R., Bessho, Y., Nakanishi, S., & Mizuno, N. (1994).
Differential expression of five N-methyl-D-aspartate receptor subunit mRNAs in
the cerebellum of developing and adult rats. Journal of Comparative Neurology.
Wiley-Blackwell. https://doi.org/10.1002/cne.903470112
chicago: Akazawa, Chihiro, Ryuichi Shigemoto, Yasumasa Bessho, Shigetada Nakanishi,
and Noboru Mizuno. “Differential Expression of Five N-Methyl-D-Aspartate Receptor
Subunit MRNAs in the Cerebellum of Developing and Adult Rats.” Journal of Comparative
Neurology. Wiley-Blackwell, 1994. https://doi.org/10.1002/cne.903470112.
ieee: C. Akazawa, R. Shigemoto, Y. Bessho, S. Nakanishi, and N. Mizuno, “Differential
expression of five N-methyl-D-aspartate receptor subunit mRNAs in the cerebellum
of developing and adult rats,” Journal of Comparative Neurology, vol. 347,
no. 1. Wiley-Blackwell, pp. 150–160, 1994.
ista: Akazawa C, Shigemoto R, Bessho Y, Nakanishi S, Mizuno N. 1994. Differential
expression of five N-methyl-D-aspartate receptor subunit mRNAs in the cerebellum
of developing and adult rats. Journal of Comparative Neurology. 347(1), 150–160.
mla: Akazawa, Chihiro, et al. “Differential Expression of Five N-Methyl-D-Aspartate
Receptor Subunit MRNAs in the Cerebellum of Developing and Adult Rats.” Journal
of Comparative Neurology, vol. 347, no. 1, Wiley-Blackwell, 1994, pp. 150–60,
doi:10.1002/cne.903470112.
short: C. Akazawa, R. Shigemoto, Y. Bessho, S. Nakanishi, N. Mizuno, Journal of
Comparative Neurology 347 (1994) 150–160.
date_created: 2018-12-11T11:57:58Z
date_published: 1994-09-01T00:00:00Z
date_updated: 2022-06-09T12:11:20Z
day: '01'
doi: 10.1002/cne.903470112
extern: '1'
external_id:
pmid:
- '7798379'
intvolume: ' 347'
issue: '1'
language:
- iso: eng
main_file_link:
- url: https://onlinelibrary.wiley.com/doi/10.1002/cne.903470112
month: '09'
oa_version: None
page: 150 - 160
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4412'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Differential expression of five N-methyl-D-aspartate receptor subunit mRNAs
in the cerebellum of developing and adult rats
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 347
year: '1994'
...
---
_id: '2549'
abstract:
- lang: eng
text: In an attempt to reveal the function sites of substance P (SP) in the central
nervous system (CNS), the distribution of SP receptor (SPR) was immunocytochemically
investigated in adult rat and compared with that of SP- positive fibers. SPR-like
immunoreactivity (LI) was mostly localized to neuronal cell bodies and dendrites.
Neurons with intense SPR-LI were distributed densely in the cortical amygdaloid
nucleus, hilus of the dentate gyrus, locus ceruleus, rostral half of the ambiguus
nucleus, and intermediolateral nucleus of the thoracic cord; moderately in the
caudatoputamen, nucleus accumbens, olfactory tubercle, median, pontine, and magnus
raphe nuclei, laminae I and III of the caudal subnucleus of the spinal trigeminal
nucleus, and lamina I of the spinal cord; and sparsely in the cerebral cortex,
basal nucleus of Meynert, claustrum, gigantocellular reticular nucleus, and lobules
IX and X of the cerebellar vermis. Neurons with weak to moderate SPR-LI were distributed
more widely throughout the CNS. The regional patterns of distribution of SPR-LI
were not necessarily the same as those of SP-positive fibers. The entopeduncular
nucleus, substantia nigra, and lateral part of the interpeduncular nucleus showed
intense SP-LI but displayed almost no SPR-LI. Conversely, the hilus of the dentate
gyrus, anterodorsal thalamic nucleus, central nucleus of the inferior colliculus,
and dorsal tegmental nucleus showed intense to moderate SPR-LI but contained few
axons with SP-LI. These findings confirmed the presence of the 'mismatch' problem
between SP and SPR localizations. However, the distribution of SPR- LI was quite
consistent with that of the SP-binding activity, which has been studied via autoradiography.
This indicates that the sites of SPR-LI revealed in the present study represent
most, if not all, sites of SP-binding activity.
acknowledgement: 'We are grateful for photographic help of Mr. A. Uesugi and the support
of Drs. M. Arakawa, S. Fukuchi, T., Fukuda, R. Hayashi, S. Hayashi, S. Imai, M.
Katsurada, Y. Kitani, K. Kumagai, H. Kuroda, T. Kuroda, H. Matsubara, J. Matsuoka,
H. Matsushima, M. Nishio, G. Niwa, H. Oda, M. Ohbayashi, S. Ohbayashi, H. Ohtsuka,
S. Tamaki, E. Watanabe, and Y. Yoshino. This work was supported in part by Grants-in-Aid
for Scientific Research on Priority Areas 05248207 and 05267104 and Scientific Research
(B) 05454658 and (C) 05680658 from the Ministry of Education, Science and Culture
of Japan. '
article_processing_charge: No
article_type: original
author:
- first_name: Yoshifumi
full_name: Nakaya, Yoshifumi
last_name: Nakaya
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Nakaya Y, Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. Immunohistochemical
localization of substance P receptor in the central nervous system of the adult
rat. Journal of Comparative Neurology. 1994;347(2):249-274. doi:10.1002/cne.903470208
apa: Nakaya, Y., Kaneko, T., Shigemoto, R., Nakanishi, S., & Mizuno, N. (1994).
Immunohistochemical localization of substance P receptor in the central nervous
system of the adult rat. Journal of Comparative Neurology. Wiley-Blackwell.
https://doi.org/10.1002/cne.903470208
chicago: Nakaya, Yoshifumi, Takeshi Kaneko, Ryuichi Shigemoto, Shigetada Nakanishi,
and Noboru Mizuno. “Immunohistochemical Localization of Substance P Receptor in
the Central Nervous System of the Adult Rat.” Journal of Comparative Neurology.
Wiley-Blackwell, 1994. https://doi.org/10.1002/cne.903470208.
ieee: Y. Nakaya, T. Kaneko, R. Shigemoto, S. Nakanishi, and N. Mizuno, “Immunohistochemical
localization of substance P receptor in the central nervous system of the adult
rat,” Journal of Comparative Neurology, vol. 347, no. 2. Wiley-Blackwell,
pp. 249–274, 1994.
ista: Nakaya Y, Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. 1994. Immunohistochemical
localization of substance P receptor in the central nervous system of the adult
rat. Journal of Comparative Neurology. 347(2), 249–274.
mla: Nakaya, Yoshifumi, et al. “Immunohistochemical Localization of Substance P
Receptor in the Central Nervous System of the Adult Rat.” Journal of Comparative
Neurology, vol. 347, no. 2, Wiley-Blackwell, 1994, pp. 249–74, doi:10.1002/cne.903470208.
short: Y. Nakaya, T. Kaneko, R. Shigemoto, S. Nakanishi, N. Mizuno, Journal of Comparative
Neurology 347 (1994) 249–274.
date_created: 2018-12-11T11:58:20Z
date_published: 1994-09-08T00:00:00Z
date_updated: 2022-06-09T09:25:30Z
day: '08'
doi: 10.1002/cne.903470208
extern: '1'
external_id:
pmid:
- '7814667'
intvolume: ' 347'
issue: '2'
language:
- iso: eng
main_file_link:
- url: https://onlinelibrary.wiley.com/doi/10.1002/cne.903470208
month: '09'
oa_version: None
page: 249 - 274
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4349'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Immunohistochemical localization of substance P receptor in the central nervous
system of the adult rat
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 347
year: '1994'
...
---
_id: '2487'
abstract:
- lang: eng
text: Distribution of the mRNA for a metabotropic glutamate receptor, mGluR3, which
is coupled to the inhibitory cAMP cascade, was examined in the central nervous
system of the adult albino rat by in situ hybridization. The hybridization signals
of mGluR3 were detected not only on neuronal cells but also on many glial cells
throughout the brain and spinal cord. In the neuronal cells, prominent expression
of mGluR3 mRNA was seen in the thalamic reticular nucleus. Moderately labeled
neurons were seen in the anterior olfactory nucleus, cerebral neo- and mesocortical
regions, lateral amygdaloid nucleus, ventral part of the basolateral amygdaloid
nucleus, dorsal endopiriform nucleus, supraoptic nucleus, superficial layers of
the superior colliculus, inferior colliculus, interpeduncular nucleus, superior
olivary nuclei, and Golgi cells in the cerebellar cortex. Weakly labeled neurons
were observed in the striatum, nucleus accumbens, ventral pallidum, globus pallidus,
entopeduncular nucleus, lateral hypothalamic area, hypothalamic paraventricular
nucleus, medial habenular nucleus, anterior pretectal nucleus, Barrington's nucleus,
Nucleus O, paragenual nucleus, trigeminal sensory complex, cochlear nuclei, dorsal
motor nucleus of the trigeminal nerve, dorsal cap of the inferior olive, spinal
dorsal horn, and lamina X of the spinal cord. The stellate cells in the cerebellar
cortex, and neurons in the deep cerebellar nuclei were also labeled weakly. The
granule cell layer of the dentate gyrus, as a whole, appeared to be labeled intensely,
but each of the granule cells was labeled only weakly. No significant labeling
was detected in the mitral and tufted cells in the olfactory bulb, hippocampal
pyramidal cells, Purkinje and granule cells in the cerebellar cortex, or somatic
motoneurons. The distribution of mGluR3 mRNA in particular neurons and glial cells
indicates specific roles of mGluR3 in the glutamatergic system of the central
nervous system.
acknowledgement: We are grateful for the photographic help of Mr. Akira Uesugi and
the support of Drs. Satoru Fukuchi, Toshio Fukuda, Ritsu Hayashi, Mizuho Katsurada,
Yutaka Kitani, Keiko Kumagai, Toshihiko Kuroda, Hiroshi Matsubara, Hiroshi Matsushima,
Chisato Minakuchi, Masatoshi Nishio, Gonpei Niwa, Hajime Oda, Masahiko Ohbayashi,
Sei-ichi Ohbayashi, Hiroyasu Ohtsuka, Shigeo Tamaki, Eizo Watan- abe, Kazuo Yoshino,
and Toshiaki Yoshino. This work was supported in part by grants-in-aid from the
Ministry of Education, Science, and Culture of Japan.
article_processing_charge: No
article_type: original
author:
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Ohishi H, Shigemoto R, Nakanishi S, Mizuno N. Distribution of the mRNA for
a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ hybridization
study. Journal of Comparative Neurology. 1993;335(2):252-266. doi:10.1002/cne.903350209'
apa: 'Ohishi, H., Shigemoto, R., Nakanishi, S., & Mizuno, N. (1993). Distribution
of the mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An
in situ hybridization study. Journal of Comparative Neurology. Wiley-Blackwell.
https://doi.org/10.1002/cne.903350209'
chicago: 'Ohishi, Hitoshi, Ryuichi Shigemoto, Shigetada Nakanishi, and Noboru Mizuno.
“ Distribution of the MRNA for a Metabotropic Glutamate Receptor (MGluR3) in the
Rat Brain: An in Situ Hybridization Study.” Journal of Comparative Neurology.
Wiley-Blackwell, 1993. https://doi.org/10.1002/cne.903350209.'
ieee: 'H. Ohishi, R. Shigemoto, S. Nakanishi, and N. Mizuno, “ Distribution of the
mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ
hybridization study,” Journal of Comparative Neurology, vol. 335, no. 2.
Wiley-Blackwell, pp. 252–266, 1993.'
ista: 'Ohishi H, Shigemoto R, Nakanishi S, Mizuno N. 1993. Distribution of the
mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ
hybridization study. Journal of Comparative Neurology. 335(2), 252–266.'
mla: 'Ohishi, Hitoshi, et al. “ Distribution of the MRNA for a Metabotropic Glutamate
Receptor (MGluR3) in the Rat Brain: An in Situ Hybridization Study.” Journal
of Comparative Neurology, vol. 335, no. 2, Wiley-Blackwell, 1993, pp. 252–66,
doi:10.1002/cne.903350209.'
short: H. Ohishi, R. Shigemoto, S. Nakanishi, N. Mizuno, Journal of Comparative
Neurology 335 (1993) 252–266.
date_created: 2018-12-11T11:57:57Z
date_published: 1993-09-08T00:00:00Z
date_updated: 2022-06-01T11:58:11Z
day: '08'
doi: 10.1002/cne.903350209
extern: '1'
external_id:
pmid:
- '8227517'
intvolume: ' 335'
issue: '2'
language:
- iso: eng
main_file_link:
- url: https://onlinelibrary.wiley.com/doi/10.1002/cne.903350209
month: '09'
oa_version: None
page: 252 - 266
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4414'
quality_controlled: '1'
status: public
title: ' Distribution of the mRNA for a metabotropic glutamate receptor (mGluR3) in
the rat brain: An in situ hybridization study'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 335
year: '1993'
...
---
_id: '2486'
abstract:
- lang: eng
text: Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1), which
is linked to phosphoinositide (PI) hydrolysis, was investigated in adult and developing
rat central nervous system (CNS) by in situ hybridization. Transcripts of mGluR1
were specifically localized to neurons and widely distributed throughout the adult
rat brain. Most intensely labeled neurons were Purkinje cells of the cerebellum,
mitral and tufted cells of the olfactory bulb, and neurons in the hippocampus,
lateral septum, thalamus, globus pallidus, entopeduncular nucleus, ventral pallidum,
magnocellular preoptic nucleus, substantia nigra, and dorsal cochlear nucleus.
Moderately labeled neurons were seen in high density in the dentate gyrus, striatum,
islands of Calleja, superficial layers of the retrosplenial, cingulate and entorhinal
cortices, mammillary nuclei, red nucleus, and superior colliculus. In the developing
rat brain, the level of mGluR1 expression gradually increased during early postnatal
days in accordance with the maturation of neuronal elements. These results show
prominent expression of mGluR1 in the major targets of putative glutamatergic
pathways and unique distribution pattern of mGluR1 distinct from those reported
for ionotropic subtypes of glutamate receptors, suggesting specific roles of mGluR1
in the glutamatergic system.
acknowledgement: We are grateful to Mr. Akira Uesugi for photographic help. This
work was supported in part by research grants from Senri Life Science Foundation
and the Ministry of Education, Science and Culture of Japan.
article_processing_charge: No
article_type: original
author:
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Shigemoto R, Nakanishi S, Mizuno N. Distribution of the mRNA for a metabotropic
glutamate receptor (mGluR1) in the central nervous system: An in situ hybridization
study in adult and developing rat. Journal of Comparative Neurology. 1992;322(1):121-135.
doi:10.1002/cne.903220110'
apa: 'Shigemoto, R., Nakanishi, S., & Mizuno, N. (1992). Distribution of the
mRNA for a metabotropic glutamate receptor (mGluR1) in the central nervous system:
An in situ hybridization study in adult and developing rat. Journal of Comparative
Neurology. Wiley-Blackwell. https://doi.org/10.1002/cne.903220110'
chicago: 'Shigemoto, Ryuichi, Shigetada Nakanishi, and Noboru Mizuno. “Distribution
of the MRNA for a Metabotropic Glutamate Receptor (MGluR1) in the Central Nervous
System: An in Situ Hybridization Study in Adult and Developing Rat.” Journal
of Comparative Neurology. Wiley-Blackwell, 1992. https://doi.org/10.1002/cne.903220110.'
ieee: 'R. Shigemoto, S. Nakanishi, and N. Mizuno, “Distribution of the mRNA for
a metabotropic glutamate receptor (mGluR1) in the central nervous system: An in
situ hybridization study in adult and developing rat,” Journal of Comparative
Neurology, vol. 322, no. 1. Wiley-Blackwell, pp. 121–135, 1992.'
ista: 'Shigemoto R, Nakanishi S, Mizuno N. 1992. Distribution of the mRNA for a
metabotropic glutamate receptor (mGluR1) in the central nervous system: An in
situ hybridization study in adult and developing rat. Journal of Comparative Neurology.
322(1), 121–135.'
mla: 'Shigemoto, Ryuichi, et al. “Distribution of the MRNA for a Metabotropic Glutamate
Receptor (MGluR1) in the Central Nervous System: An in Situ Hybridization Study
in Adult and Developing Rat.” Journal of Comparative Neurology, vol. 322,
no. 1, Wiley-Blackwell, 1992, pp. 121–35, doi:10.1002/cne.903220110.'
short: R. Shigemoto, S. Nakanishi, N. Mizuno, Journal of Comparative Neurology 322
(1992) 121–135.
date_created: 2018-12-11T11:57:57Z
date_published: 1992-08-01T00:00:00Z
date_updated: 2022-03-21T09:41:37Z
day: '01'
doi: 10.1002/cne.903220110
extern: '1'
external_id:
pmid:
- '1430307'
intvolume: ' 322'
issue: '1'
language:
- iso: eng
main_file_link:
- url: https://onlinelibrary.wiley.com/doi/10.1002/cne.903220110
month: '08'
oa_version: Published Version
page: 121 - 135
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4415'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1) in
the central nervous system: An in situ hybridization study in adult and developing
rat'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 322
year: '1992'
...