---
_id: '11975'
abstract:
- lang: eng
  text: A new method was developed for the quantitative analysis of steryl glycosides
    in biodiesel (fatty acid methyl esters). This method is much more sensitive than
    existing methods and has minimum limits of quantification of 50 μg/kg, compared
    to previously published minimum limits of quantification of about 15 mg/kg. The
    analysis is based on gas chromatography–mass spectroscopy determination of simple
    pre-treated and silylated samples via single ion monitoring at 147, 204, 217 m/z,
    which are specific ions for the silylated sugar moiety. Quantification was carried
    out using cholesteryl β-d-glucopyranoside as internal standard. The modified synthesis
    and purification of the internal standard is also presented as well as the characterization
    by NMR and mass spectroscopy. The advantage of the method compared with other
    approaches is the simplified sample preparation avoiding extra pre-treatment steps
    coupled with complete derivatization of the sugar hydroxyl groups by using N,O-bis(trimethylsilyl)acetamide
    with 5% trimethylchlorosilane as derivatization reagent. On the given conditions
    high recovery rates ≥89% can be obtained. Evaluation of lab specific variance
    and intermediate precision underline the robustness of the method which will be
    further assessed by Round robin tests.
article_processing_charge: No
article_type: original
author:
- first_name: Bartholomäus
  full_name: Pieber, Bartholomäus
  id: 93e5e5b2-0da6-11ed-8a41-af589a024726
  last_name: Pieber
  orcid: 0000-0001-8689-388X
- first_name: Sigurd
  full_name: Schober, Sigurd
  last_name: Schober
- first_name: Christoph
  full_name: Goebl, Christoph
  last_name: Goebl
- first_name: Martin
  full_name: Mittelbach, Martin
  last_name: Mittelbach
citation:
  ama: Pieber B, Schober S, Goebl C, Mittelbach M. Novel sensitive determination of
    steryl glycosides in biodiesel by gas chromatography-mass spectroscopy. <i>Journal
    of Chromatography A</i>. 2010;1217(42):6555-6561. doi:<a href="https://doi.org/10.1016/j.chroma.2010.08.006">10.1016/j.chroma.2010.08.006</a>
  apa: Pieber, B., Schober, S., Goebl, C., &#38; Mittelbach, M. (2010). Novel sensitive
    determination of steryl glycosides in biodiesel by gas chromatography-mass spectroscopy.
    <i>Journal of Chromatography A</i>. Elsevier. <a href="https://doi.org/10.1016/j.chroma.2010.08.006">https://doi.org/10.1016/j.chroma.2010.08.006</a>
  chicago: Pieber, Bartholomäus, Sigurd Schober, Christoph Goebl, and Martin Mittelbach.
    “Novel Sensitive Determination of Steryl Glycosides in Biodiesel by Gas Chromatography-Mass
    Spectroscopy.” <i>Journal of Chromatography A</i>. Elsevier, 2010. <a href="https://doi.org/10.1016/j.chroma.2010.08.006">https://doi.org/10.1016/j.chroma.2010.08.006</a>.
  ieee: B. Pieber, S. Schober, C. Goebl, and M. Mittelbach, “Novel sensitive determination
    of steryl glycosides in biodiesel by gas chromatography-mass spectroscopy,” <i>Journal
    of Chromatography A</i>, vol. 1217, no. 42. Elsevier, pp. 6555–6561, 2010.
  ista: Pieber B, Schober S, Goebl C, Mittelbach M. 2010. Novel sensitive determination
    of steryl glycosides in biodiesel by gas chromatography-mass spectroscopy. Journal
    of Chromatography A. 1217(42), 6555–6561.
  mla: Pieber, Bartholomäus, et al. “Novel Sensitive Determination of Steryl Glycosides
    in Biodiesel by Gas Chromatography-Mass Spectroscopy.” <i>Journal of Chromatography
    A</i>, vol. 1217, no. 42, Elsevier, 2010, pp. 6555–61, doi:<a href="https://doi.org/10.1016/j.chroma.2010.08.006">10.1016/j.chroma.2010.08.006</a>.
  short: B. Pieber, S. Schober, C. Goebl, M. Mittelbach, Journal of Chromatography
    A 1217 (2010) 6555–6561.
date_created: 2022-08-25T10:43:43Z
date_published: 2010-10-15T00:00:00Z
date_updated: 2023-02-21T10:09:59Z
day: '15'
doi: 10.1016/j.chroma.2010.08.006
extern: '1'
external_id:
  pmid:
  - '20846658'
intvolume: '      1217'
issue: '42'
language:
- iso: eng
month: '10'
oa_version: None
page: 6555-6561
pmid: 1
publication: Journal of Chromatography A
publication_identifier:
  issn:
  - 0021-9673
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Novel sensitive determination of steryl glycosides in biodiesel by gas chromatography-mass
  spectroscopy
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1217
year: '2010'
...