[{"type":"journal_article","author":[{"last_name":"Loose","full_name":"Loose, Martin","first_name":"Martin","orcid":"0000-0001-7309-9724","id":"462D4284-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-3580-2906","id":"3018E8C2-F248-11E8-B48F-1D18A9856A87","full_name":"Auer, Albert","first_name":"Albert","last_name":"Auer"},{"last_name":"Brognara","first_name":"Gabriel","full_name":"Brognara, Gabriel","id":"D96FFDA0-A884-11E9-9968-DC26E6697425"},{"last_name":"Budiman","first_name":"Hanifatul R","full_name":"Budiman, Hanifatul R","id":"55380f95-15b2-11ec-abd3-aff8e230696b"},{"id":"e3a512e2-4bbe-11eb-a68a-e3857a7844c2","last_name":"Kowalski","first_name":"Lukasz M","full_name":"Kowalski, Lukasz M"},{"id":"83c17ce3-15b2-11ec-abd3-f486545870bd","first_name":"Ivana","full_name":"Matijevic, Ivana","last_name":"Matijevic"}],"day":"01","title":"In vitro reconstitution of small GTPase regulation","citation":{"chicago":"Loose, Martin, Albert Auer, Gabriel Brognara, Hanifatul R Budiman, Lukasz M Kowalski, and Ivana Matijevic. “In Vitro Reconstitution of Small GTPase Regulation.” <i>FEBS Letters</i>. Wiley, 2023. <a href=\"https://doi.org/10.1002/1873-3468.14540\">https://doi.org/10.1002/1873-3468.14540</a>.","ieee":"M. Loose, A. Auer, G. Brognara, H. R. Budiman, L. M. Kowalski, and I. Matijevic, “In vitro reconstitution of small GTPase regulation,” <i>FEBS Letters</i>, vol. 597, no. 6. Wiley, pp. 762–777, 2023.","ista":"Loose M, Auer A, Brognara G, Budiman HR, Kowalski LM, Matijevic I. 2023. In vitro reconstitution of small GTPase regulation. FEBS Letters. 597(6), 762–777.","mla":"Loose, Martin, et al. “In Vitro Reconstitution of Small GTPase Regulation.” <i>FEBS Letters</i>, vol. 597, no. 6, Wiley, 2023, pp. 762–77, doi:<a href=\"https://doi.org/10.1002/1873-3468.14540\">10.1002/1873-3468.14540</a>.","short":"M. Loose, A. Auer, G. Brognara, H.R. Budiman, L.M. Kowalski, I. Matijevic, FEBS Letters 597 (2023) 762–777.","ama":"Loose M, Auer A, Brognara G, Budiman HR, Kowalski LM, Matijevic I. In vitro reconstitution of small GTPase regulation. <i>FEBS Letters</i>. 2023;597(6):762-777. doi:<a href=\"https://doi.org/10.1002/1873-3468.14540\">10.1002/1873-3468.14540</a>","apa":"Loose, M., Auer, A., Brognara, G., Budiman, H. R., Kowalski, L. M., &#38; Matijevic, I. (2023). In vitro reconstitution of small GTPase regulation. <i>FEBS Letters</i>. Wiley. <a href=\"https://doi.org/10.1002/1873-3468.14540\">https://doi.org/10.1002/1873-3468.14540</a>"},"ddc":["570"],"doi":"10.1002/1873-3468.14540","language":[{"iso":"eng"}],"keyword":["Cell Biology","Genetics","Molecular Biology","Biochemistry","Structural Biology","Biophysics"],"acknowledgement":"The authors acknowledge support from IST Austria and helpful comments from the anonymous reviewers that helped to improve this manuscript. We apologize to the authors of primary literature and outstanding research not cited here due to space restraints.","pmid":1,"date_created":"2023-01-12T12:09:58Z","month":"03","page":"762-777","intvolume":"       597","status":"public","quality_controlled":"1","department":[{"_id":"MaLo"}],"publication":"FEBS Letters","isi":1,"publisher":"Wiley","has_accepted_license":"1","oa_version":"Published Version","year":"2023","article_type":"review","publication_identifier":{"eissn":["1873-3468"],"issn":["0014-5793"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2023-08-16T08:32:29Z","external_id":{"pmid":["36448231"],"isi":["000891573000001"]},"scopus_import":"1","_id":"12163","date_published":"2023-03-01T00:00:00Z","abstract":[{"text":"Small GTPases play essential roles in the organization of eukaryotic cells. In recent years, it has become clear that their intracellular functions result from intricate biochemical networks of the GTPase and their regulators that dynamically bind to a membrane surface. Due to the inherent complexities of their interactions, however, revealing the underlying mechanisms of action is often difficult to achieve from in vivo studies. This review summarizes in vitro reconstitution approaches developed to obtain a better mechanistic understanding of how small GTPase activities are regulated in space and time.","lang":"eng"}],"file":[{"file_name":"2023_FEBSLetters_Loose.pdf","file_size":3148143,"creator":"dernst","date_updated":"2023-08-16T08:31:04Z","access_level":"open_access","checksum":"7492244d3f9c5faa1347ef03f6e5bc84","content_type":"application/pdf","relation":"main_file","file_id":"14063","success":1,"date_created":"2023-08-16T08:31:04Z"}],"issue":"6","article_processing_charge":"Yes (via OA deal)","volume":597,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"file_date_updated":"2023-08-16T08:31:04Z","oa":1,"publication_status":"published","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/"},{"volume":429,"publication_status":"published","date_published":"1998-06-05T00:00:00Z","_id":"1954","abstract":[{"lang":"eng","text":"\r\nWe have examined the effects of heat stress on electron transfer in the thylakoid membrane of an engineered plastid ndh deletion mutant, Δ1, incapable of performing the Ndh-mediated reduction of the plastoquinone pool in the chloroplast. Upon heat stress in the dark, the rate of PSII- independent reduction of PSI after subsequent illumination by far-red light is dramatically enhanced in both Δ1 and a wild-type control plant (WT). In contrast, in the dark, only the WT shows an increase in the reduction state of the plastoquinone pool. We conclude that the heat stress-induced reduction of the intersystem electron transport chain can be mediated by Ndh- independent pathways in the light but that in the dark the dominant pathway for reduction of the plastoquinone pool is catalysed by the Ndh complex. Our results therefore demonstrate a functional role for the Ndh complex in the dark.\r\n"}],"article_processing_charge":"No","issue":"1","external_id":{"pmid":["9657394 "]},"date_updated":"2022-09-01T13:12:15Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publication_identifier":{"issn":["0014-5793"]},"article_type":"original","publist_id":"5128","oa_version":"None","year":"1998","publication":"FEBS Letters","quality_controlled":"1","status":"public","intvolume":"       429","publisher":"Elsevier","month":"06","extern":"1","date_created":"2018-12-11T11:54:54Z","page":"115 - 118","language":[{"iso":"eng"}],"doi":"10.1016/S0014-5793(98)00573-0","pmid":1,"acknowledgement":"This work was funded by the BBSRC. We would like to thank Professor Peter Horton (University of Sheffield) for the loan of the ED 800 T unit.","day":"05","author":[{"first_name":"Leonid A","full_name":"Sazanov, Leonid A","last_name":"Sazanov","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0977-7989"},{"last_name":"Burrows","full_name":"Burrows, Paul","first_name":"Paul"},{"full_name":"Nixon, Peter","first_name":"Peter","last_name":"Nixon"}],"type":"journal_article","citation":{"ama":"Sazanov LA, Burrows P, Nixon P. The chloroplast Ndh complex mediates the dark reduction of the plastoquinone pool in response to heat stress in tobacco leaves. <i>FEBS Letters</i>. 1998;429(1):115-118. doi:<a href=\"https://doi.org/10.1016/S0014-5793(98)00573-0\">10.1016/S0014-5793(98)00573-0</a>","apa":"Sazanov, L. A., Burrows, P., &#38; Nixon, P. (1998). The chloroplast Ndh complex mediates the dark reduction of the plastoquinone pool in response to heat stress in tobacco leaves. <i>FEBS Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/S0014-5793(98)00573-0\">https://doi.org/10.1016/S0014-5793(98)00573-0</a>","short":"L.A. Sazanov, P. Burrows, P. Nixon, FEBS Letters 429 (1998) 115–118.","mla":"Sazanov, Leonid A., et al. “The Chloroplast Ndh Complex Mediates the Dark Reduction of the Plastoquinone Pool in Response to Heat Stress in Tobacco Leaves.” <i>FEBS Letters</i>, vol. 429, no. 1, Elsevier, 1998, pp. 115–18, doi:<a href=\"https://doi.org/10.1016/S0014-5793(98)00573-0\">10.1016/S0014-5793(98)00573-0</a>.","chicago":"Sazanov, Leonid A, Paul Burrows, and Peter Nixon. “The Chloroplast Ndh Complex Mediates the Dark Reduction of the Plastoquinone Pool in Response to Heat Stress in Tobacco Leaves.” <i>FEBS Letters</i>. Elsevier, 1998. <a href=\"https://doi.org/10.1016/S0014-5793(98)00573-0\">https://doi.org/10.1016/S0014-5793(98)00573-0</a>.","ieee":"L. A. Sazanov, P. Burrows, and P. Nixon, “The chloroplast Ndh complex mediates the dark reduction of the plastoquinone pool in response to heat stress in tobacco leaves,” <i>FEBS Letters</i>, vol. 429, no. 1. Elsevier, pp. 115–118, 1998.","ista":"Sazanov LA, Burrows P, Nixon P. 1998. The chloroplast Ndh complex mediates the dark reduction of the plastoquinone pool in response to heat stress in tobacco leaves. FEBS Letters. 429(1), 115–118."},"title":"The chloroplast Ndh complex mediates the dark reduction of the plastoquinone pool in response to heat stress in tobacco leaves"},{"doi":"10.1016/0014-5793(94)00370-X","language":[{"iso":"eng"}],"acknowledgement":"LAS is grateful to the Wellcome Trust for a fellowship. We should like to thank Prof. R.M. Denton for discussion.","pmid":1,"type":"journal_article","author":[{"first_name":"Leonid A","full_name":"Sazanov, Leonid A","last_name":"Sazanov","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0977-7989"},{"full_name":"Jackson, Julie","first_name":"Julie","last_name":"Jackson"}],"day":"16","title":"Proton translocating transhydrogenase and NAD- and NADP-linked isocitrate dehydrogenases operate in a substrate cycle which contributes to fine regulation of the tricarboxylic acid cycle activity in mitochondria","citation":{"mla":"Sazanov, Leonid A., and Julie Jackson. “Proton Translocating Transhydrogenase and NAD- and NADP-Linked Isocitrate Dehydrogenases Operate in a Substrate Cycle Which Contributes to Fine Regulation of the Tricarboxylic Acid Cycle Activity in Mitochondria.” <i>FEBS Letters</i>, vol. 344, no. 2–3, Elsevier, 1994, pp. 109–16, doi:<a href=\"https://doi.org/10.1016/0014-5793(94)00370-X\">10.1016/0014-5793(94)00370-X</a>.","chicago":"Sazanov, Leonid A, and Julie Jackson. “Proton Translocating Transhydrogenase and NAD- and NADP-Linked Isocitrate Dehydrogenases Operate in a Substrate Cycle Which Contributes to Fine Regulation of the Tricarboxylic Acid Cycle Activity in Mitochondria.” <i>FEBS Letters</i>. Elsevier, 1994. <a href=\"https://doi.org/10.1016/0014-5793(94)00370-X\">https://doi.org/10.1016/0014-5793(94)00370-X</a>.","ista":"Sazanov LA, Jackson J. 1994. Proton translocating transhydrogenase and NAD- and NADP-linked isocitrate dehydrogenases operate in a substrate cycle which contributes to fine regulation of the tricarboxylic acid cycle activity in mitochondria. FEBS Letters. 344(2–3), 109–116.","ieee":"L. A. Sazanov and J. Jackson, “Proton translocating transhydrogenase and NAD- and NADP-linked isocitrate dehydrogenases operate in a substrate cycle which contributes to fine regulation of the tricarboxylic acid cycle activity in mitochondria,” <i>FEBS Letters</i>, vol. 344, no. 2–3. Elsevier, pp. 109–116, 1994.","ama":"Sazanov LA, Jackson J. Proton translocating transhydrogenase and NAD- and NADP-linked isocitrate dehydrogenases operate in a substrate cycle which contributes to fine regulation of the tricarboxylic acid cycle activity in mitochondria. <i>FEBS Letters</i>. 1994;344(2-3):109-116. doi:<a href=\"https://doi.org/10.1016/0014-5793(94)00370-X\">10.1016/0014-5793(94)00370-X</a>","apa":"Sazanov, L. A., &#38; Jackson, J. (1994). Proton translocating transhydrogenase and NAD- and NADP-linked isocitrate dehydrogenases operate in a substrate cycle which contributes to fine regulation of the tricarboxylic acid cycle activity in mitochondria. <i>FEBS Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/0014-5793(94)00370-X\">https://doi.org/10.1016/0014-5793(94)00370-X</a>","short":"L.A. Sazanov, J. Jackson, FEBS Letters 344 (1994) 109–116."},"status":"public","intvolume":"       344","quality_controlled":"1","publication":"FEBS Letters","publisher":"Elsevier","date_created":"2018-12-11T11:54:52Z","extern":"1","month":"05","page":"109 - 116","publication_identifier":{"issn":["0014-5793"]},"date_updated":"2022-06-09T13:21:50Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","external_id":{"pmid":["8187868"]},"oa_version":"Published Version","year":"1994","publist_id":"5134","article_type":"original","volume":344,"main_file_link":[{"url":"https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2894%2900370-X","open_access":"1"}],"oa":1,"publication_status":"published","date_published":"1994-05-16T00:00:00Z","_id":"1949","abstract":[{"lang":"eng","text":"H+-transhydrogenase (H+-Thase) and NADP-linked isocitrate dehydrogenase (NADP-ICDH) are very active in animal mitochondria but their physiological function is only poorly understood. This is especially so in the case of the heart and muscle, where there are no major consumers of NADPH. We propose here that H+-Thase and NADP-ICDH have a combined function in the fine regulation of the activity of the tricarboxylic acid (TCA) cycle, providing enhanced sensitivy to changes in energy demand. This is achieved through cycling of substrates by NAD-linked ICDH, NADP-linked ICDH and H+-Thase. It is proposed that NAD-ICDH operates in the forward direction of the TCA cycle, but NADP-ICDH is driven in reverse by elevated levels of NADPH resulting from the action of the transmembrane proton electrochemical potential gradient (Δp) on H+-Thase. This has the effect of increasing the sensitivity to allosteric modifiers of NAD-ICDH (NADH, ADP, ATP, Ca2+ etc), potentially giving rise to large changes in the net flux from iso-citrate to α-ketoglutarate. Furthermore, changes in the level of Δp resulting from changes in the demand for ATP would, via H+-Thase, shift the redox state of the NADP pool and this, in turn, would lead to a change in the rate of the reaction catalysed by NADP-ICDH and hence to an additional and complementary effect on the net metabolic flux from isocitrate to α-ketoglutarate. Other consequences of this substrate cycle are, (i) the production of heat at the expense of Δp, which may contribute to thermoregulation in the animal, and (ii) an increased rate of dissipation of Δp (leak)."}],"issue":"2-3","article_processing_charge":"No"},{"volume":355,"publication_status":"published","oa":1,"main_file_link":[{"open_access":"1","url":"https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2894%2901109-5"}],"_id":"1953","abstract":[{"lang":"eng","text":"The respiratory burst induced by phorbol myristate acetate in mouse macrophages was inhibited by ultra-low doses (10-15 -10-13 M) of an opioid peptide [d-Ala2] methionine enkephalinamide. The effect disappeared at concentrations above and below this range. The inhibition approached 50% and was statistically significant (P &lt; 0.001). Increasing the time of the opioid incubation with cells brought about a shift in the maximal effect to lower concentrations of the opioid (from 10-13 to 5 · 10-15 M) and led to a decrease in the value of the effect, fully in accord with the previously proposed adaptation mechanism of the action of ultra-low doses."}],"date_published":"1994-11-28T00:00:00Z","article_processing_charge":"No","issue":"2","external_id":{"pmid":["7982481"]},"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_updated":"2022-06-09T12:58:57Z","publication_identifier":{"issn":["0014-5793"]},"article_type":"original","publist_id":"5133","oa_version":"Published Version","year":"1994","publication":"FEBS Letters","quality_controlled":"1","status":"public","intvolume":"       355","publisher":"Elsevier","month":"11","extern":"1","date_created":"2018-12-11T11:54:53Z","page":"114 - 116","language":[{"iso":"eng"}],"doi":"10.1016/0014-5793(94)01109-5","pmid":1,"day":"28","type":"journal_article","author":[{"last_name":"Efanov","first_name":"Alexander","full_name":"Efanov, Alexander"},{"last_name":"Koshkin","full_name":"Koshkin, Aleksei","first_name":"Aleksei"},{"id":"338D39FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0977-7989","last_name":"Sazanov","full_name":"Sazanov, Leonid A","first_name":"Leonid A"},{"last_name":"Borodulina","first_name":"O I","full_name":"Borodulina, O I"},{"last_name":"Varfolomeev","first_name":"Sergei","full_name":"Varfolomeev, Sergei"},{"first_name":"Sergei","full_name":"Zaǐtsev, Sergei","last_name":"Zaǐtsev"}],"citation":{"chicago":"Efanov, Alexander, Aleksei Koshkin, Leonid A Sazanov, O I Borodulina, Sergei Varfolomeev, and Sergei Zaǐtsev. “Inhibition of the Respiratory Burst in Mouse Macrophages by Ultra-Low Doses of an Opioid Peptide Is Consistent with a Possible Adaptation Mechanism.” <i>FEBS Letters</i>. Elsevier, 1994. <a href=\"https://doi.org/10.1016/0014-5793(94)01109-5\">https://doi.org/10.1016/0014-5793(94)01109-5</a>.","ista":"Efanov A, Koshkin A, Sazanov LA, Borodulina OI, Varfolomeev S, Zaǐtsev S. 1994. Inhibition of the respiratory burst in mouse macrophages by ultra-low doses of an opioid peptide is consistent with a possible adaptation mechanism. FEBS Letters. 355(2), 114–116.","ieee":"A. Efanov, A. Koshkin, L. A. Sazanov, O. I. Borodulina, S. Varfolomeev, and S. Zaǐtsev, “Inhibition of the respiratory burst in mouse macrophages by ultra-low doses of an opioid peptide is consistent with a possible adaptation mechanism,” <i>FEBS Letters</i>, vol. 355, no. 2. Elsevier, pp. 114–116, 1994.","mla":"Efanov, Alexander, et al. “Inhibition of the Respiratory Burst in Mouse Macrophages by Ultra-Low Doses of an Opioid Peptide Is Consistent with a Possible Adaptation Mechanism.” <i>FEBS Letters</i>, vol. 355, no. 2, Elsevier, 1994, pp. 114–16, doi:<a href=\"https://doi.org/10.1016/0014-5793(94)01109-5\">10.1016/0014-5793(94)01109-5</a>.","short":"A. Efanov, A. Koshkin, L.A. Sazanov, O.I. Borodulina, S. Varfolomeev, S. Zaǐtsev, FEBS Letters 355 (1994) 114–116.","ama":"Efanov A, Koshkin A, Sazanov LA, Borodulina OI, Varfolomeev S, Zaǐtsev S. Inhibition of the respiratory burst in mouse macrophages by ultra-low doses of an opioid peptide is consistent with a possible adaptation mechanism. <i>FEBS Letters</i>. 1994;355(2):114-116. doi:<a href=\"https://doi.org/10.1016/0014-5793(94)01109-5\">10.1016/0014-5793(94)01109-5</a>","apa":"Efanov, A., Koshkin, A., Sazanov, L. A., Borodulina, O. I., Varfolomeev, S., &#38; Zaǐtsev, S. (1994). Inhibition of the respiratory burst in mouse macrophages by ultra-low doses of an opioid peptide is consistent with a possible adaptation mechanism. <i>FEBS Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/0014-5793(94)01109-5\">https://doi.org/10.1016/0014-5793(94)01109-5</a>"},"title":"Inhibition of the respiratory burst in mouse macrophages by ultra-low doses of an opioid peptide is consistent with a possible adaptation mechanism"}]