---
_id: '1946'
abstract:
- lang: eng
text: An ultra-low dose (10-14 M) of opioid peptide [D-Ala2]methionine enkephalinamide
(DAMEA) is found to exert an inhibitory effect on the production of reactive oxygen
species (respiratory burst) in human neutrophils. The validity of this phenomenon
has been verified in a series of studies that comprised 30 experiments. The inhibition
has proved to be statistically significant (P<0.001). The dose-response dependence
of the effect (10-15-10-9 M) followed a characteristic biphasic pattern (with
the maximum effect at ultra-low doses). An opioid antagonist, naloxone partially
blocks the inhibitory effect, which indicates that the DAMEA action is at least
partially mediated by opioid receptors.
article_processing_charge: No
author:
- first_name: Sergei
full_name: Zaǐtsev, Sergei
last_name: Zaǐtsev
- first_name: Leonid A
full_name: Sazanov, Leonid A
id: 338D39FE-F248-11E8-B48F-1D18A9856A87
last_name: Sazanov
orcid: 0000-0002-0977-7989
- first_name: Aleksei
full_name: Koshkin, Aleksei
last_name: Koshkin
- first_name: Galina
full_name: Sud'Ina, Galina
last_name: Sud'Ina
- first_name: Sergei
full_name: Varfolomeev, Sergei
last_name: Varfolomeev
citation:
ama: Zaǐtsev S, Sazanov LA, Koshkin A, Sud’Ina G, Varfolomeev S. Respiratory burst
inhibition in human neutrophils by ultra-low doses of [D-Ala2] methionine enkephalinamide.
FEBS Letters. 1991;291(1):84-86. doi:10.1016/0014-5793(91)81109-L
apa: Zaǐtsev, S., Sazanov, L. A., Koshkin, A., Sud’Ina, G., & Varfolomeev, S.
(1991). Respiratory burst inhibition in human neutrophils by ultra-low doses of
[D-Ala2] methionine enkephalinamide. FEBS Letters. Elsevier. https://doi.org/10.1016/0014-5793(91)81109-L
chicago: Zaǐtsev, Sergei, Leonid A Sazanov, Aleksei Koshkin, Galina Sud’Ina, and
Sergei Varfolomeev. “Respiratory Burst Inhibition in Human Neutrophils by Ultra-Low
Doses of [D-Ala2] Methionine Enkephalinamide.” FEBS Letters. Elsevier,
1991. https://doi.org/10.1016/0014-5793(91)81109-L.
ieee: S. Zaǐtsev, L. A. Sazanov, A. Koshkin, G. Sud’Ina, and S. Varfolomeev, “Respiratory
burst inhibition in human neutrophils by ultra-low doses of [D-Ala2] methionine
enkephalinamide,” FEBS Letters, vol. 291, no. 1. Elsevier, pp. 84–86, 1991.
ista: Zaǐtsev S, Sazanov LA, Koshkin A, Sud’Ina G, Varfolomeev S. 1991. Respiratory
burst inhibition in human neutrophils by ultra-low doses of [D-Ala2] methionine
enkephalinamide. FEBS Letters. 291(1), 84–86.
mla: Zaǐtsev, Sergei, et al. “Respiratory Burst Inhibition in Human Neutrophils
by Ultra-Low Doses of [D-Ala2] Methionine Enkephalinamide.” FEBS Letters,
vol. 291, no. 1, Elsevier, 1991, pp. 84–86, doi:10.1016/0014-5793(91)81109-L.
short: S. Zaǐtsev, L.A. Sazanov, A. Koshkin, G. Sud’Ina, S. Varfolomeev, FEBS Letters
291 (1991) 84–86.
date_created: 2018-12-11T11:54:51Z
date_published: 1991-10-07T00:00:00Z
date_updated: 2022-03-07T09:49:06Z
day: '07'
doi: 10.1016/0014-5793(91)81109-L
extern: '1'
external_id:
pmid:
- '1936255 '
intvolume: ' 291'
issue: '1'
language:
- iso: eng
main_file_link:
- url: https://www.sciencedirect.com/science/article/pii/001457939181109L
month: '10'
oa_version: None
page: 84 - 86
pmid: 1
publication: FEBS Letters
publication_identifier:
issn:
- 0014-2956
publication_status: published
publisher: Elsevier
publist_id: '5139'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Respiratory burst inhibition in human neutrophils by ultra-low doses of [D-Ala2]
methionine enkephalinamide
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 291
year: '1991'
...
---
_id: '2481'
abstract:
- lang: eng
text: The family of mammalian tachykinin receptors consists of substance P receptor
(SPR), neuromedin K receptor (NKR) and substance K receptor (SKR). In this investigation,
tissue and regional distributions of the mRNAs for the three rat tachykinin receptors
were investigated by blot-hybridization and RNase-protection analyses using the
previously cloned receptor cDNAs. SPR mRNA is widely distributed in both the nervous
system and peripheral tissues and is expressed abundantly in the hypothalamus
and olfactory buld, as well as in the urinary bladder, salivary glands and small
and large intestines. In contrast, NKR mRNA is predominantly expressed in the
nervous system, particularly in the cortex, hypothalamus and cerebellum, whereas
SKR mRNA expression is restricted to the peripheral tissues, being abundant in
the urinary bladder, large intestine, stomach and adenal glands. Thus, the mRNAs
for the three tachykinin receptors show distinct patterns of expression between
the nervous system and peripheral tissues. Blot-hybridization analysis in combination
with S1 nuclease protection and primer-extension analyses revealed that there
are two large forms of SKR mRNA expressed commonly in the peripheral tissues,
and two additional small forms of the mRNA expressed specifically in the adrenal
gland and eye. These analyses also showed that the multiple forms of SKR mRNA
differ in the lengths of the 5' mRNA portions, and that the two small forms of
the mRNA, if translated, encode a truncated SKR polypeptide lacking the first
two transmembrane domains. This investigation thus provides the comprehensive
analysis of the distribution and mode of expression of the mRNAs for the multiple
peptide receptors and offers a new basis on which to interpret the diverse functions
of multiple tachykinin peptides in the CNS and peripheral tissues.
acknowledgement: This work was supported in part by research grants from the Ministry
of Education, Science and Culture of Japan, the Ministry of Health and Welfare
of Japan, and the Yamanouchi Foundation for Research on Metabolic Disorders
article_processing_charge: No
article_type: original
author:
- first_name: Kunihiro
full_name: Tsuchida, Kunihiro
last_name: Tsuchida
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Yoshifumi
full_name: Yokota, Yoshifumi
last_name: Yokota
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
citation:
ama: Tsuchida K, Shigemoto R, Yokota Y, Nakanishi S. Tissue distribution and quantitation
of the mRNAs for three rat tachykinin receptors. European Journal of Biochemistry.
1990;193(3):751-757. doi:10.1111/j.1432-1033.1990.tb19396.x
apa: Tsuchida, K., Shigemoto, R., Yokota, Y., & Nakanishi, S. (1990). Tissue
distribution and quantitation of the mRNAs for three rat tachykinin receptors.
European Journal of Biochemistry. Wiley-Blackwell. https://doi.org/10.1111/j.1432-1033.1990.tb19396.x
chicago: Tsuchida, Kunihiro, Ryuichi Shigemoto, Yoshifumi Yokota, and Shigetada
Nakanishi. “Tissue Distribution and Quantitation of the MRNAs for Three Rat Tachykinin
Receptors.” European Journal of Biochemistry. Wiley-Blackwell, 1990. https://doi.org/10.1111/j.1432-1033.1990.tb19396.x.
ieee: K. Tsuchida, R. Shigemoto, Y. Yokota, and S. Nakanishi, “Tissue distribution
and quantitation of the mRNAs for three rat tachykinin receptors,” European
Journal of Biochemistry, vol. 193, no. 3. Wiley-Blackwell, pp. 751–757, 1990.
ista: Tsuchida K, Shigemoto R, Yokota Y, Nakanishi S. 1990. Tissue distribution
and quantitation of the mRNAs for three rat tachykinin receptors. European Journal
of Biochemistry. 193(3), 751–757.
mla: Tsuchida, Kunihiro, et al. “Tissue Distribution and Quantitation of the MRNAs
for Three Rat Tachykinin Receptors.” European Journal of Biochemistry,
vol. 193, no. 3, Wiley-Blackwell, 1990, pp. 751–57, doi:10.1111/j.1432-1033.1990.tb19396.x.
short: K. Tsuchida, R. Shigemoto, Y. Yokota, S. Nakanishi, European Journal of Biochemistry
193 (1990) 751–757.
date_created: 2018-12-11T11:57:55Z
date_published: 1990-03-03T00:00:00Z
date_updated: 2022-02-24T10:20:14Z
day: '03'
doi: 10.1111/j.1432-1033.1990.tb19396.x
extern: '1'
external_id:
pmid:
- '1701145'
intvolume: ' 193'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1990.tb19396.x
month: '03'
oa: 1
oa_version: Published Version
page: 751 - 757
pmid: 1
publication: European Journal of Biochemistry
publication_identifier:
eissn:
- 1432-1033
issn:
- 0014-2956
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4420'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tissue distribution and quantitation of the mRNAs for three rat tachykinin
receptors
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 193
year: '1990'
...