---
_id: '6507'
abstract:
- lang: eng
text: The osteoclast-associated receptor (OSCAR) is a collagen-binding immune receptor
with important roles in dendritic cell maturation and activation of inflammatory
monocytes as well as in osteoclastogenesis. The crystal structure of the OSCAR
ectodomain is presented, both free and in complex with a consensus triple-helical
peptide (THP). The structures revealed a collagen-binding site in each immunoglobulin-like
domain (D1 and D2). The THP binds near a predicted collagen-binding groove in
D1, but a more extensive interaction with D2 is facilitated by the unusually wide
D1-D2 interdomain angle in OSCAR. Direct binding assays, combined with site-directed
mutagenesis, confirm that the primary collagen-binding site in OSCAR resides in
D2, in marked contrast to the related collagen receptors, glycoprotein VI (GPVI)
and leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1). Monomeric OSCAR
D1D2 binds to the consensus THP with a KD of 28 µM measured in solution, but shows
a higher affinity (KD 1.5 μM) when binding to a solid-phase THP, most likely due
to an avidity effect. These data suggest a 2-stage model for the interaction of
OSCAR with a collagen fibril, with transient, low-affinity interactions initiated
by the membrane-distal D1, followed by firm adhesion to the primary binding site
in D2.
author:
- first_name: Long
full_name: Zhou, Long
id: 3E751364-F248-11E8-B48F-1D18A9856A87
last_name: Zhou
orcid: 0000-0002-1864-8951
- first_name: J. M.
full_name: Hinerman, J. M.
last_name: Hinerman
- first_name: M.
full_name: Blaszczyk, M.
last_name: Blaszczyk
- first_name: J. L. C.
full_name: Miller, J. L. C.
last_name: Miller
- first_name: D. G.
full_name: Conrady, D. G.
last_name: Conrady
- first_name: A. D.
full_name: Barrow, A. D.
last_name: Barrow
- first_name: D. Y.
full_name: Chirgadze, D. Y.
last_name: Chirgadze
- first_name: D.
full_name: Bihan, D.
last_name: Bihan
- first_name: R. W.
full_name: Farndale, R. W.
last_name: Farndale
- first_name: A. B.
full_name: Herr, A. B.
last_name: Herr
citation:
ama: Zhou L, Hinerman JM, Blaszczyk M, et al. Structural basis for collagen recognition
by the immune receptor OSCAR. Blood. 2015;127(5):529-537. doi:10.1182/blood-2015-08-667055
apa: Zhou, L., Hinerman, J. M., Blaszczyk, M., Miller, J. L. C., Conrady, D. G.,
Barrow, A. D., … Herr, A. B. (2015). Structural basis for collagen recognition
by the immune receptor OSCAR. Blood. American Society of Hematology. https://doi.org/10.1182/blood-2015-08-667055
chicago: Zhou, Long, J. M. Hinerman, M. Blaszczyk, J. L. C. Miller, D. G. Conrady,
A. D. Barrow, D. Y. Chirgadze, D. Bihan, R. W. Farndale, and A. B. Herr. “Structural
Basis for Collagen Recognition by the Immune Receptor OSCAR.” Blood. American
Society of Hematology, 2015. https://doi.org/10.1182/blood-2015-08-667055.
ieee: L. Zhou et al., “Structural basis for collagen recognition by the immune
receptor OSCAR,” Blood, vol. 127, no. 5. American Society of Hematology,
pp. 529–537, 2015.
ista: Zhou L, Hinerman JM, Blaszczyk M, Miller JLC, Conrady DG, Barrow AD, Chirgadze
DY, Bihan D, Farndale RW, Herr AB. 2015. Structural basis for collagen recognition
by the immune receptor OSCAR. Blood. 127(5), 529–537.
mla: Zhou, Long, et al. “Structural Basis for Collagen Recognition by the Immune
Receptor OSCAR.” Blood, vol. 127, no. 5, American Society of Hematology,
2015, pp. 529–37, doi:10.1182/blood-2015-08-667055.
short: L. Zhou, J.M. Hinerman, M. Blaszczyk, J.L.C. Miller, D.G. Conrady, A.D. Barrow,
D.Y. Chirgadze, D. Bihan, R.W. Farndale, A.B. Herr, Blood 127 (2015) 529–537.
date_created: 2019-05-31T09:38:50Z
date_published: 2015-11-02T00:00:00Z
date_updated: 2021-01-12T08:07:47Z
day: '02'
doi: 10.1182/blood-2015-08-667055
extern: '1'
external_id:
pmid:
- '26552697'
intvolume: ' 127'
issue: '5'
language:
- iso: eng
month: '11'
oa_version: None
page: 529-537
pmid: 1
publication: Blood
publication_identifier:
issn:
- 0006-4971
- 1528-0020
publication_status: published
publisher: American Society of Hematology
quality_controlled: '1'
status: public
title: Structural basis for collagen recognition by the immune receptor OSCAR
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 127
year: '2015'
...
---
_id: '4153'
article_processing_charge: No
article_type: original
author:
- first_name: D.
full_name: Ransom, D.
last_name: Ransom
- first_name: Alison
full_name: Brownlie, Alison
last_name: Brownlie
- first_name: Pascal
full_name: Haffter, Pascal
last_name: Haffter
- first_name: Jörg
full_name: Odenthal, Jörg
last_name: Odenthal
- first_name: Robert
full_name: Kelsh, Robert
last_name: Kelsh
- first_name: Michael
full_name: Brand, Michael
last_name: Brand
- first_name: Makoto
full_name: Furutani Seiki, Makoto
last_name: Furutani Seiki
- first_name: Michael
full_name: Granato, Michael
last_name: Granato
- first_name: Matthias
full_name: Hammerschmidt, Matthias
last_name: Hammerschmidt
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Yunjin
full_name: Jiang, Yunjin
last_name: Jiang
- first_name: David
full_name: Kane, David
last_name: Kane
- first_name: Mary
full_name: Mullins, Mary
last_name: Mullins
- first_name: Fredericus
full_name: Van Eden, Fredericus
last_name: Van Eden
- first_name: Rachel
full_name: Warga, Rachel
last_name: Warga
- first_name: Christiane
full_name: Nüsslein Volhard, Christiane
last_name: Nüsslein Volhard
- first_name: L.
full_name: Zon, L.
last_name: Zon
citation:
ama: Ransom D, Brownlie A, Haffter P, et al. Hematopoietic mutants identified in
a saturation screen of the zebrafish genome. Blood. 1995;86(10):1912-1912.
apa: Ransom, D., Brownlie, A., Haffter, P., Odenthal, J., Kelsh, R., Brand, M.,
… Zon, L. (1995). Hematopoietic mutants identified in a saturation screen of the
zebrafish genome. Blood. American Society of Hematology.
chicago: Ransom, D., Alison Brownlie, Pascal Haffter, Jörg Odenthal, Robert Kelsh,
Michael Brand, Makoto Furutani Seiki, et al. “Hematopoietic Mutants Identified
in a Saturation Screen of the Zebrafish Genome.” Blood. American Society
of Hematology, 1995.
ieee: D. Ransom et al., “Hematopoietic mutants identified in a saturation
screen of the zebrafish genome,” Blood, vol. 86, no. 10. American Society
of Hematology, pp. 1912–1912, 1995.
ista: Ransom D, Brownlie A, Haffter P, Odenthal J, Kelsh R, Brand M, Furutani Seiki
M, Granato M, Hammerschmidt M, Heisenberg C-PJ, Jiang Y, Kane D, Mullins M, Van
Eden F, Warga R, Nüsslein Volhard C, Zon L. 1995. Hematopoietic mutants identified
in a saturation screen of the zebrafish genome. Blood. 86(10), 1912–1912.
mla: Ransom, D., et al. “Hematopoietic Mutants Identified in a Saturation Screen
of the Zebrafish Genome.” Blood, vol. 86, no. 10, American Society of Hematology,
1995, pp. 1912–1912.
short: D. Ransom, A. Brownlie, P. Haffter, J. Odenthal, R. Kelsh, M. Brand, M. Furutani
Seiki, M. Granato, M. Hammerschmidt, C.-P.J. Heisenberg, Y. Jiang, D. Kane, M.
Mullins, F. Van Eden, R. Warga, C. Nüsslein Volhard, L. Zon, Blood 86 (1995) 1912–1912.
date_created: 2018-12-11T12:07:16Z
date_published: 1995-01-01T00:00:00Z
date_updated: 2022-06-13T12:02:52Z
day: '01'
extern: '1'
intvolume: ' 86'
issue: '10'
language:
- iso: eng
month: '01'
oa_version: None
page: 1912 - 1912
publication: Blood
publication_identifier:
issn:
- 0006-4971
publication_status: published
publisher: American Society of Hematology
publist_id: '1965'
quality_controlled: '1'
status: public
title: Hematopoietic mutants identified in a saturation screen of the zebrafish genome
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 86
year: '1995'
...