---
_id: '1958'
abstract:
- lang: eng
  text: "\r\nComplex I (NADH:ubiquinone oxidoreductase) purified from bovine heart
    mitochondria was treated with the detergent N,N-dimethyldodecylamine N-oxide (LDAO).
    The enzyme dissociated into two known subcomplexes, Iα and Iβ, containing mostly
    hydrophilic and hydrophobic subunits, and a previously undetected fragment referred
    to as Iγ. Subcomplex Iγ contains the hydrophobic subunits ND1, ND2, ND3, and ND4L
    which are encoded in the mitochondrial genome, and the nuclear-encoded subunit
    KFYL. During size- exclusion chromatography in the presence of LDAO, subcomplex
    Iα lost several subunits and formed another characterized subcomplex known as
    Iλ. Similarly, subcomplex Iβ dissociated into two smaller subcomplexes, one of
    which contains the hydrophobic subunits ND4 and ND5; subcomplex Iγ released a
    fragment containing ND1 and ND2. These results suggest that in the intact complex
    subunits ND1 and ND2 are likely to be in a different region of the membrane domain
    than subunits ND4 and ND5. The compositions of the various subcomplexes and fragments
    of complex I provide an organization of the subunits of the enzyme in the framework
    of the known low resolution structure of the enzyme."
article_processing_charge: No
article_type: original
author:
- first_name: Leonid A
  full_name: Sazanov, Leonid A
  id: 338D39FE-F248-11E8-B48F-1D18A9856A87
  last_name: Sazanov
  orcid: 0000-0002-0977-7989
- first_name: Sew
  full_name: Peak Chew, Sew
  last_name: Peak Chew
- first_name: Ian
  full_name: Fearnley, Ian
  last_name: Fearnley
- first_name: John
  full_name: Walker, John
  last_name: Walker
citation:
  ama: 'Sazanov LA, Peak Chew S, Fearnley I, Walker J. Resolution of the membrane
    domain of bovine complex I into subcomplexes: implications for the structural
    organization of the enzyme. <i>Biochemistry</i>. 2000;39(24):7229-7235. doi:<a
    href="https://doi.org/10.1021/bi000335t">10.1021/bi000335t</a>'
  apa: 'Sazanov, L. A., Peak Chew, S., Fearnley, I., &#38; Walker, J. (2000). Resolution
    of the membrane domain of bovine complex I into subcomplexes: implications for
    the structural organization of the enzyme. <i>Biochemistry</i>. ACS. <a href="https://doi.org/10.1021/bi000335t">https://doi.org/10.1021/bi000335t</a>'
  chicago: 'Sazanov, Leonid A, Sew Peak Chew, Ian Fearnley, and John Walker. “Resolution
    of the Membrane Domain of Bovine Complex I into Subcomplexes: Implications for
    the Structural Organization of the Enzyme.” <i>Biochemistry</i>. ACS, 2000. <a
    href="https://doi.org/10.1021/bi000335t">https://doi.org/10.1021/bi000335t</a>.'
  ieee: 'L. A. Sazanov, S. Peak Chew, I. Fearnley, and J. Walker, “Resolution of the
    membrane domain of bovine complex I into subcomplexes: implications for the structural
    organization of the enzyme,” <i>Biochemistry</i>, vol. 39, no. 24. ACS, pp. 7229–7235,
    2000.'
  ista: 'Sazanov LA, Peak Chew S, Fearnley I, Walker J. 2000. Resolution of the membrane
    domain of bovine complex I into subcomplexes: implications for the structural
    organization of the enzyme. Biochemistry. 39(24), 7229–7235.'
  mla: 'Sazanov, Leonid A., et al. “Resolution of the Membrane Domain of Bovine Complex
    I into Subcomplexes: Implications for the Structural Organization of the Enzyme.”
    <i>Biochemistry</i>, vol. 39, no. 24, ACS, 2000, pp. 7229–35, doi:<a href="https://doi.org/10.1021/bi000335t">10.1021/bi000335t</a>.'
  short: L.A. Sazanov, S. Peak Chew, I. Fearnley, J. Walker, Biochemistry 39 (2000)
    7229–7235.
date_created: 2018-12-11T11:54:55Z
date_published: 2000-05-24T00:00:00Z
date_updated: 2023-05-04T14:14:53Z
day: '24'
doi: 10.1021/bi000335t
extern: '1'
external_id:
  pmid:
  - '10852722'
intvolume: '        39'
issue: '24'
language:
- iso: eng
month: '05'
oa_version: None
page: 7229 - 7235
pmid: 1
publication: Biochemistry
publication_identifier:
  issn:
  - 0006-2960
publication_status: published
publisher: ACS
publist_id: '5127'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Resolution of the membrane domain of bovine complex I into subcomplexes: implications
  for the structural organization of the enzyme'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 39
year: '2000'
...