---
_id: '10900'
abstract:
- lang: eng
  text: Leukocyte migration through the interstitial space is crucial for the maintenance
    of tolerance and immunity. The main cues for leukocyte trafficking are chemokines
    thought to directionally guide these cells towards their targets. However, model
    systems that facilitate quantification of chemokine-guided leukocyte migration
    in vivo are uncommon. Here we describe an ex vivo crawl-in assay using explanted
    mouse ears that allows the visualization of chemokine-dependent dendritic cell
    (DC) motility in the dermal interstitium in real time. We present methods for
    the preparation of mouse ear sheets and their use in multidimensional confocal
    imaging experiments to monitor and analyze the directional migration of fluorescently
    labelled DCs through the dermis and into afferent lymphatic vessels. The assay
    provides a more physiological approach to study leukocyte migration than in vitro
    three-dimensional (3D) or 2-dimensional (2D) migration assays such as collagen
    gels and transwell assays.
acknowledgement: We would like to thank Alexander Eichner and Ingrid de Vries for
  discussion and critical reading of the manuscript, and Mary Frank for assistance
  with the recording of videos and images in Fig. 1. M.S. is supported through funding
  from the German Research Foundation (DFG). M.W. acknowledges the Alexander von Humboldt
  Foundation for funding.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Michele
  full_name: Weber, Michele
  id: 3A3FC708-F248-11E8-B48F-1D18A9856A87
  last_name: Weber
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: 'Weber M, Sixt MK. Live Cell Imaging of Chemotactic Dendritic Cell Migration
    in Explanted Mouse Ear Preparations. In: Cardona A, Ubogu E, eds. <i>Chemokines</i>.
    Vol 1013. MIMB. Totowa, NJ: Humana Press; 2013:215-226. doi:<a href="https://doi.org/10.1007/978-1-62703-426-5_14">10.1007/978-1-62703-426-5_14</a>'
  apa: 'Weber, M., &#38; Sixt, M. K. (2013). Live Cell Imaging of Chemotactic Dendritic
    Cell Migration in Explanted Mouse Ear Preparations. In A. Cardona &#38; E. Ubogu
    (Eds.), <i>Chemokines</i> (Vol. 1013, pp. 215–226). Totowa, NJ: Humana Press.
    <a href="https://doi.org/10.1007/978-1-62703-426-5_14">https://doi.org/10.1007/978-1-62703-426-5_14</a>'
  chicago: 'Weber, Michele, and Michael K Sixt. “Live Cell Imaging of Chemotactic
    Dendritic Cell Migration in Explanted Mouse Ear Preparations.” In <i>Chemokines</i>,
    edited by Astrid Cardona and Eroboghene Ubogu, 1013:215–26. MIMB. Totowa, NJ:
    Humana Press, 2013. <a href="https://doi.org/10.1007/978-1-62703-426-5_14">https://doi.org/10.1007/978-1-62703-426-5_14</a>.'
  ieee: 'M. Weber and M. K. Sixt, “Live Cell Imaging of Chemotactic Dendritic Cell
    Migration in Explanted Mouse Ear Preparations,” in <i>Chemokines</i>, vol. 1013,
    A. Cardona and E. Ubogu, Eds. Totowa, NJ: Humana Press, 2013, pp. 215–226.'
  ista: 'Weber M, Sixt MK. 2013.Live Cell Imaging of Chemotactic Dendritic Cell Migration
    in Explanted Mouse Ear Preparations. In: Chemokines. Methods in Molecular Biology,
    vol. 1013, 215–226.'
  mla: Weber, Michele, and Michael K. Sixt. “Live Cell Imaging of Chemotactic Dendritic
    Cell Migration in Explanted Mouse Ear Preparations.” <i>Chemokines</i>, edited
    by Astrid Cardona and Eroboghene Ubogu, vol. 1013, Humana Press, 2013, pp. 215–26,
    doi:<a href="https://doi.org/10.1007/978-1-62703-426-5_14">10.1007/978-1-62703-426-5_14</a>.
  short: M. Weber, M.K. Sixt, in:, A. Cardona, E. Ubogu (Eds.), Chemokines, Humana
    Press, Totowa, NJ, 2013, pp. 215–226.
date_created: 2022-03-21T07:47:41Z
date_published: 2013-04-03T00:00:00Z
date_updated: 2023-09-05T13:15:33Z
day: '03'
department:
- _id: MiSi
doi: 10.1007/978-1-62703-426-5_14
editor:
- first_name: Astrid
  full_name: Cardona, Astrid
  last_name: Cardona
- first_name: Eroboghene
  full_name: Ubogu, Eroboghene
  last_name: Ubogu
external_id:
  pmid:
  - '23625502'
intvolume: '      1013'
language:
- iso: eng
month: '04'
oa_version: None
page: 215-226
place: Totowa, NJ
pmid: 1
publication: Chemokines
publication_identifier:
  eisbn:
  - '9781627034265'
  eissn:
  - 1940-6029
  isbn:
  - '9781627034258'
  issn:
  - 1064-3745
publication_status: published
publisher: Humana Press
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse
  Ear Preparations
type: book_chapter
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 1013
year: '2013'
...