---
_id: '7010'
abstract:
- lang: eng
  text: Numerous biophysical questions require the quantification of short-range interactions
    between (functionalized) surfaces and synthetic or biological objects such as
    cells. Here, we present an original, custom built setup for reflection interference
    contrast microscopy that can assess distances between a substrate and a flowing
    object at high speed with nanometric accuracy. We demonstrate its use to decipher
    the complex biochemical and mechanical interplay regulating blood cell homing
    at the vessel wall in the microcirculation using an in vitro approach. We show
    that in the absence of specific biochemical interactions, flowing cells are repelled
    from the soft layer lining the vessel wall, contributing to red blood cell repulsion
    in vivo. In contrast, this so-called glycocalyx stabilizes rolling of cells under
    flow in the presence of a specific receptor naturally present on activated leucocytes
    and a number of cancer cell lines.
article_number: 110760V
article_processing_charge: No
author:
- first_name: Heather S.
  full_name: Davies, Heather S.
  last_name: Davies
- first_name: Natalia S.
  full_name: Baranova, Natalia S.
  id: 38661662-F248-11E8-B48F-1D18A9856A87
  last_name: Baranova
  orcid: 0000-0002-3086-9124
- first_name: Nouha
  full_name: El Amri, Nouha
  last_name: El Amri
- first_name: Liliane
  full_name: Coche-Guérente, Liliane
  last_name: Coche-Guérente
- first_name: Claude
  full_name: Verdier, Claude
  last_name: Verdier
- first_name: Lionel
  full_name: Bureau, Lionel
  last_name: Bureau
- first_name: Ralf P.
  full_name: Richter, Ralf P.
  last_name: Richter
- first_name: Delphine
  full_name: Débarre, Delphine
  last_name: Débarre
citation:
  ama: 'Davies HS, Baranova NS, El Amri N, et al. Blood cell-vessel wall interactions
    probed by reflection interference contrast microscopy. In: <i>Advances in Microscopic
    Imaging II</i>. Vol 11076. SPIE; 2019. doi:<a href="https://doi.org/10.1117/12.2527058">10.1117/12.2527058</a>'
  apa: 'Davies, H. S., Baranova, N. S., El Amri, N., Coche-Guérente, L., Verdier,
    C., Bureau, L., … Débarre, D. (2019). Blood cell-vessel wall interactions probed
    by reflection interference contrast microscopy. In <i>Advances in Microscopic
    Imaging II</i> (Vol. 11076). Munich, Germany: SPIE. <a href="https://doi.org/10.1117/12.2527058">https://doi.org/10.1117/12.2527058</a>'
  chicago: Davies, Heather S., Natalia S. Baranova, Nouha El Amri, Liliane Coche-Guérente,
    Claude Verdier, Lionel Bureau, Ralf P. Richter, and Delphine Débarre. “Blood Cell-Vessel
    Wall Interactions Probed by Reflection Interference Contrast Microscopy.” In <i>Advances
    in Microscopic Imaging II</i>, Vol. 11076. SPIE, 2019. <a href="https://doi.org/10.1117/12.2527058">https://doi.org/10.1117/12.2527058</a>.
  ieee: H. S. Davies <i>et al.</i>, “Blood cell-vessel wall interactions probed by
    reflection interference contrast microscopy,” in <i>Advances in Microscopic Imaging
    II</i>, Munich, Germany, 2019, vol. 11076.
  ista: Davies HS, Baranova NS, El Amri N, Coche-Guérente L, Verdier C, Bureau L,
    Richter RP, Débarre D. 2019. Blood cell-vessel wall interactions probed by reflection
    interference contrast microscopy. Advances in Microscopic Imaging II. European
    Conferences on Biomedical Optics vol. 11076, 110760V.
  mla: Davies, Heather S., et al. “Blood Cell-Vessel Wall Interactions Probed by Reflection
    Interference Contrast Microscopy.” <i>Advances in Microscopic Imaging II</i>,
    vol. 11076, 110760V, SPIE, 2019, doi:<a href="https://doi.org/10.1117/12.2527058">10.1117/12.2527058</a>.
  short: H.S. Davies, N.S. Baranova, N. El Amri, L. Coche-Guérente, C. Verdier, L.
    Bureau, R.P. Richter, D. Débarre, in:, Advances in Microscopic Imaging II, SPIE,
    2019.
conference:
  end_date: 2019-06-27
  location: Munich, Germany
  name: European Conferences on Biomedical Optics
  start_date: 2019-06-26
date_created: 2019-11-12T15:10:18Z
date_published: 2019-07-22T00:00:00Z
date_updated: 2023-08-29T06:54:38Z
day: '22'
department:
- _id: MaLo
doi: 10.1117/12.2527058
external_id:
  isi:
  - '000535353000023'
intvolume: '     11076'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://hal.archives-ouvertes.fr/hal-02368135/file/110760V.pdf
month: '07'
oa: 1
oa_version: Published Version
publication: Advances in Microscopic Imaging II
publication_identifier:
  isbn:
  - '9781510628458'
  issn:
  - 1605-7422
publication_status: published
publisher: SPIE
quality_controlled: '1'
scopus_import: '1'
status: public
title: Blood cell-vessel wall interactions probed by reflection interference contrast
  microscopy
type: conference
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11076
year: '2019'
...