---
_id: '6178'
abstract:
- lang: eng
  text: Mechanically coupled cells can generate forces driving cell and tissue morphogenesis
    during development. Visualization and measuring of these forces is of major importance
    to better understand the complexity of the biomechanic processes that shape cells
    and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively
    assess mechanical tension in different tissues of the developing zebrafish and
    in cultures of primary germ layer progenitor cells ex vivo.
article_processing_charge: No
author:
- first_name: Michael
  full_name: Smutny, Michael
  id: 3FE6E4E8-F248-11E8-B48F-1D18A9856A87
  last_name: Smutny
  orcid: 0000-0002-5920-9090
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Pedro
  full_name: Campinho, Pedro
  id: 3AFBBC42-F248-11E8-B48F-1D18A9856A87
  last_name: Campinho
  orcid: 0000-0002-8526-5416
- first_name: Verena
  full_name: Ruprecht, Verena
  id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
  last_name: Ruprecht
  orcid: 0000-0003-4088-8633
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: 'Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. UV laser ablation
    to measure cell and tissue-generated forces in the zebrafish embryo in vivo and
    ex vivo. In: Nelson C, ed. <i>Tissue Morphogenesis</i>. Vol 1189. Methods in Molecular
    Biology. New York, NY: Springer; 2014:219-235. doi:<a href="https://doi.org/10.1007/978-1-4939-1164-6_15">10.1007/978-1-4939-1164-6_15</a>'
  apa: 'Smutny, M., Behrndt, M., Campinho, P., Ruprecht, V., &#38; Heisenberg, C.-P.
    J. (2014). UV laser ablation to measure cell and tissue-generated forces in the
    zebrafish embryo in vivo and ex vivo. In C. Nelson (Ed.), <i>Tissue Morphogenesis</i>
    (Vol. 1189, pp. 219–235). New York, NY: Springer. <a href="https://doi.org/10.1007/978-1-4939-1164-6_15">https://doi.org/10.1007/978-1-4939-1164-6_15</a>'
  chicago: 'Smutny, Michael, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and
    Carl-Philipp J Heisenberg. “UV Laser Ablation to Measure Cell and Tissue-Generated
    Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” In <i>Tissue Morphogenesis</i>,
    edited by Celeste Nelson, 1189:219–35. Methods in Molecular Biology. New York,
    NY: Springer, 2014. <a href="https://doi.org/10.1007/978-1-4939-1164-6_15">https://doi.org/10.1007/978-1-4939-1164-6_15</a>.'
  ieee: 'M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, and C.-P. J. Heisenberg,
    “UV laser ablation to measure cell and tissue-generated forces in the zebrafish
    embryo in vivo and ex vivo,” in <i>Tissue Morphogenesis</i>, vol. 1189, C. Nelson,
    Ed. New York, NY: Springer, 2014, pp. 219–235.'
  ista: 'Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. 2014.UV laser
    ablation to measure cell and tissue-generated forces in the zebrafish embryo in
    vivo and ex vivo. In: Tissue Morphogenesis. vol. 1189, 219–235.'
  mla: Smutny, Michael, et al. “UV Laser Ablation to Measure Cell and Tissue-Generated
    Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” <i>Tissue Morphogenesis</i>,
    edited by Celeste Nelson, vol. 1189, Springer, 2014, pp. 219–35, doi:<a href="https://doi.org/10.1007/978-1-4939-1164-6_15">10.1007/978-1-4939-1164-6_15</a>.
  short: M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, C.-P.J. Heisenberg, in:,
    C. Nelson (Ed.), Tissue Morphogenesis, Springer, New York, NY, 2014, pp. 219–235.
date_created: 2019-03-26T08:55:59Z
date_published: 2014-08-22T00:00:00Z
date_updated: 2023-09-05T14:12:00Z
day: '22'
department:
- _id: CaHe
doi: 10.1007/978-1-4939-1164-6_15
editor:
- first_name: Celeste
  full_name: Nelson, Celeste
  last_name: Nelson
external_id:
  pmid:
  - '25245697'
intvolume: '      1189'
language:
- iso: eng
month: '08'
oa_version: None
page: 219-235
place: New York, NY
pmid: 1
publication: Tissue Morphogenesis
publication_identifier:
  eissn:
  - 1940-6029
  isbn:
  - '9781493911639'
  - '9781493911646'
  issn:
  - 1064-3745
publication_status: published
publisher: Springer
quality_controlled: '1'
series_title: Methods in Molecular Biology
status: public
title: UV laser ablation to measure cell and tissue-generated forces in the zebrafish
  embryo in vivo and ex vivo
type: book_chapter
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 1189
year: '2014'
...